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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Industri Pertanian VALENSI Jurnal Veteriner JURNAL KIMIA SAINS DAN APLIKASI Jurnal Natur Indonesia JURNAL SAINS DAN TEKNOLOGI INDONESIA Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat AGRIVITA, Journal of Agricultural Science SEMIRATA 2015 Biology, Medicine, & Natural Product Chemistry Jurnal Sumberdaya Hayati Jurnal Kimia Mulawarman Indonesia Chimica Acta Science and Technology Indonesia Al-Kimia Indonesian Journal of Chemistry Biosaintifika: Journal of Biology & Biology Education Journal of Tropical Crop Science Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat Jurnal Agrotropika Makara Journal of Science Molekul: Jurnal Ilmiah Kimia Jurna Beta Kimia Jurnal Tumbuhan Obat Indonesia Borneo Journal of Pharmacy LIMNOTEK Journal Serambi Engineering (JSE) CEFARS : JURNAL AGRIBISNIS DAN PENGEMBANGAN WILAYAH Jurnal Agribisnis dan Pengembangan Wilayah
Dyah Iswantini
Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Veterinary

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Journal : HAYATI Journal of Biosciences

 1 
Zingiber cassumunar Roxb. (Bangle), Guazuma ulmifolia Lamk. (Jati belanda), and Murraya paniculata (Kemuning) have been used as slimming agents in jamu. A few researches have performed studies on their potency as antiobesity. The aim of this research was to investigate the potency of Z. cassumunar rhizome, G. ulmifolia, and M. paniculata leaf extracts as antiobesity agent based on in vitro inhibition activity of the extracts on pancreatic lipase activity. In this research, water content determin DYAH ISWANTINI; RHOITO FROISTA SILITONGA; ELIZABETH MARTATILOFA; LATIFAH KOSIM DARUSMAN
HAYATI Journal of Biosciences Vol. 18 No. 1 (2011): March 2011
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.1.6

Abstract

Zingiber cassumunar Roxb. (Bangle), Guazuma ulmifolia Lamk. (Jati belanda), and Murraya paniculata (Kemuning) have been used as slimming agents in jamu. A few researches have performed studies on their potency as antiobesity. The aim of this research was to investigate the potency of Z. cassumunar rhizome, G. ulmifolia, and M. paniculata leaf extracts as antiobesity agent based on in vitro inhibition activity of the extracts on pancreatic lipase activity. In this research, water content determination, phytochemical assay, toxicity assay and in vitro assay of inhibition activity on pancreatic lipase were performed toward single and mixture extracts of Z. cassumunar, G. ulmifolia, and M. paniculata resulted by water, ethanol, and saponin extractions. The results indicated that 100 ppm of ethanol extraction of Z. cassumunar had highest inhibition effect on the activity of pancreatic lipase (29.17%), followed by 100 ppm of water extraction of M. paniculata  (25.66%), 60 ppm of ethanol extraction of G. ulmifolia leaves (25.13%) and ethanol extraction mixture of Z. cassumunar, G. ulmifolia, and M. paniculata  leaves with ratio of 25:25:25 (21.58%). These inhibition effects were higher than inhibitory effect of 100 ppm of Xenical®/orlistat as the positive control, with the inhibition value of 17.53%. Saponin crude extracts had lower inhibitory effect than the other extractions. It was suggested that ethanol extraction of Z. cassumunar, and G. ulmifolia and water extraction of M. paniculata had potency as antiobesity agent
Activity and Stability of the Alcohol Biosensor Using Acetobacter aceti Biofilm on Screen-Printed Carbon Electrode Dyah Iswantini; Fitriani Indahsari; Akhiruddin Maddu; Novik Nurhidayat; Henny Purwaningsih; Sri Sugiarti
HAYATI Journal of Biosciences Vol. 27 No. 1 (2020): January 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (865.056 KB) | DOI: 10.4308/hjb.27.1.24

Abstract

Most of the alcohol analytical methods are robust and instrumentally expensive. An alternative of ethanol biosensor based on selected biofilm forming Acetobacter aceti bacteria producing alcohol oxidase was constructed on a screen-printed carbon electrode. The enzyme specifically oxidizes the ethanol and generate electrical current that then electrochemically detected and measured by cyclic voltammetry method. A scanning electron microscopic analysis indicated that the biofilm was formed firmly in the electrode. This constructed biosensor reached its optimum at biofilm formed by bacteria of 1.33 × 1010 cells/ml, temperature of 27°C, and pH 7. The enzyme kinetic had KM and Vmax AOX values of 3.5 mm and 125 μA respectively. The biosensor had detection and quantization limit of 0.003 and 0.009%, respectively, and a sensitivity of 57.29 μA (%)-1. A linearity and relative deviation value were revealed at 0.993 and 1.95% respectively. The biosensor was relatively specific and had no interferences with methanol, sodium chloride and citric acid as the common interferences of ethanol compounds. Furthermore, the biosensor had been stably for at least 55 days. Therefore, this constructed biosensor should be developed into a prototype for a practical effective analysis.
Evaluation of Bacterial Biofilm as Biosensor for Detecting Phenol, Catechol, and 1,2-dihydroxynaphthalene Dyah Iswantini; Ali Aulia Ghozali; Cecep Kusmana; Novik Nurhidayat
HAYATI Journal of Biosciences Vol. 28 No. 4 (2021): October 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.4.262-270

Abstract

Monitoring of water quality from the presence of polyaromatic hydrocarbon (PAHs) compounds and its derivates are important for keeping the healthy aquatic environment. Some of those derivates are phenol and several related compounds sharing simmilar structures. This reseach aimed for the detection of those phenol and several similar compounds monitoring due to PAHs degradation. Three identified bacterial isolates of Pseudomonas aeruginosa, Staphylococcus sciuri, and Bacillus amyloliquifaciens were selected based on their phenol degradation characters. On physiological properties all three isolates were observed to degrade several hydrophobic substances such as for naphthalene and anthracene. Yet, genetic analysis indicated that the phenolic degradating oxygenase gene was detected only in the P. aeruginosa and S. sciuri. Applying those isolates for biofilm as biosensor showed a sufficient analytical performance such as their limit of detection between 0.1-0.5 μM.
In Vitro Pancreatic Lipase Inhibition by Marine Fungi Purpureocillium lilacinum Associated with Stylissa sp. Sponge as Anti-obesity Agent Wendi Nurul Fadillah; Nampiah Sukarno; Dyah Iswantini; Min Rahminiwati; Novriyandi Hanif; Mashuri Waite
HAYATI Journal of Biosciences Vol. 29 No. 1 (2022): January 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.1.76-86

Abstract

This study aimed to evaluate the potential of marine fungus Purpureocillium lilacinum isolated from an Indonesian marine sponge Stylissa sp. as an anti-obesity agent through pancreatic lipase inhibition assay. The fungus was identified as P. lilacinum through morphological and molecular characteristics. The fungal extract’s inhibition activity and kinetics were evaluated using spectrophotometry and Lineweaver-Burk plots. Ethyl acetate and butanol were used for extraction. Both extracts showed pancreatic lipase inhibition in a concentration-dependent manner. Both crude extracts were then fractionated once. All fractionated extracts showed inhibitory activity above 50%, with the highest activity found in fraction 5 of ethyl acetate at 93.41% inhibition. The best fractionated extract had an IC50value of 220.60 µg.mL-1. The most active fraction of P. lilacinum had a competitive-type inhibitor behavior as shown by the value of Vmax not significantly changing from 388.80 to 382.62 mM pNP.min-1, and the Michaelis-Menten constant (KM) increased from 2.02 to 5.47 mM in the presence of 500 µg.mL-1 fractionated extract. Metabolite identification with LC-MS/MS QTOF suggested that galangin, kaempferol, and quercetin were responsible for the observed lipase inhibition.
Antigout Activity of The Spatholobus littoralis Hassk. Extract Fractions Against Xanthine Oxidase: Its Metabolite Profile and Inhibition Kinetics Rut Novalia Rahmawati Sianipar; Komar Sutriah; Dyah Iswantini; Trivadila; Suminar Setiati Achmadi
HAYATI Journal of Biosciences Vol. 31 No. 1 (2024): January 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.1.1-20

Abstract

The Spatholobus littoralis Hassk. (S. littoralis Hassk.) is a native plant in Indonesia and has been widely used in traditional Dayaknese medicine for non-communicable degenerative diseases. One of these illnesses, known as gout, is caused by excessive uric acid in the blood, which is the catalytic byproduct of a xanthine oxidase (XO) enzyme. In this work, we investigated the inhibition kinetics of XO and identified bioactive compounds from the stem extract fractions of S. littoralis Hassk. using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Fractionation was carried out to obtain n-hexane, dichloromethane, and 1-butanol fractions from the water and 70% ethanol extracts. Fraction of 1-butanol from 70% ethanol and water extract displayed potent inhibitors of XO with IC50 value 116.91±3.51 and 137.15±5.00 mg/L, respectively. Lineweaver-Burk plot analysis showed that the 1-butanol fraction from the two extracts inhibited XO competitively. The 1-butanol fraction from the two extracts has been further identified as a bioactive fraction. The majority of the compounds in the two active fractions were phenolics. These findings revealed that the 1-butanol fraction from the two extracts is promising as an antigout treatment in the future.
Exploring Indonesian Sponge-Associated Marine Aspergillus hortai: Characterization of Bioactive Compounds with Potential Anti-Escherichia coli Properties Fadillah, Wendi Nurul; Sukarno, Nampiah; Iswantini, Dyah; Rahminiwati, Min; Franco, Christopher MM; Zhang, Wei; Hanif, Novriyandi; Waite, Mashuri
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.712-724

Abstract

Sponge-associated marine fungi are potential source for secondary metabolite compounds. The aim of this research was to investigate sponge-associated marine fungus as secondary metabolite producers against Escherichia coli. The fungus was isolated from Indonesian marine sponge Stylissa sp. and identified as Aspergillus hortai through a combination of morphological and molecular characteristics of ITS DNA and β-tubulin genes. The fungus was tested against E. coli using fungal broth and mycelial extracts. The optimized condition was achieved by fungal broth grown in corn meal broth at 6-days of shaking incubation. Fungal extract was produced using three liters of filtered fungal broth and extracted in ethyl acetate. The antibiotic activity of the extract is vulnerable to 45°C heat and basic or acidic conditions. Therefore, the extraction was done at pH 7 with evaporation at 40°C. The extract shows 7 major bands on TLC with 1 band shows activity against E. coli (Rf 0.81) on bioautogram. The band was observed as a yellow color and turned black in short-wave UV and did not show any fluorescence in long-wave UV. This research shows that sponge-associated marine fungi obtained from Indonesia has the potential as anti E. coli worth to be explored for searching new antibiotics.
Co-Authors , Triadiati . TRIVADILA Adayani Nurjayanti Adelia, Nesha Adi Riyadhi Adiarso Afifi, Muhammad Ridho Agus Kartono Agus Sudiman Tjokrowardojo, Agus Sudiman Ahmad Sjahriza Ahmad, Shahrul Nizam Ai Susanti Akhiruddin Maddu Akmalina, Rifkah Alfiona, Della Ali Aulia Ghozali Ali Aulia Ghozali Alif, Alfiah Amalyah Febryanti Amalyah Febryanti Ana Fitriyani Anas Miftah Fauzi Anggia Murni Aninda, Yunita Anja Meryandini Bagaskoro Tuwalaid Budi Riza Putra Charlena Deden Saprudin Dewi Sondari Diah Ratnadewi Dian Siska RF Dinda Iryawati Dita Ariyanti DJUMALI MANGUNWIDJAJA Djumali Mangunwidjaja Dwiastuti, Rahma Eka Mardiah ELIZABETH MARTATILOFA Eti Rohaeti Euis Filaila Evul Winoto Evul Winoto Fadillah, Wendi Nurul Firdaus, Ridwan Fitriani Indahsari Franco, Christopher MM Fretty Yurike Fyrda Shellia Ghozali, Ali Aulia Hafshah, Nurul Haki, Horiana hanifah hanifah Harry Noviardi Hasim - Hefni Effendi Henny Purwaningsih Ida Ayu Putu Sri Widnyani Irmanida Batubara Ismet, Rhahmasari Johnson N Naat Joni Prasetyo, Joni Jovianto, Andrian Karnadi Komar Sutriah Kurniawan, Yehezkiel Steven latifah K Darusman Latifah K Darusman Latifah K. Darusman LATIFAH KOSIM DARUSMAN LATIFAH KOSIM DARUSMAN Lawa, Yosep Lenni Fitri Lenni Fitri Lestarani, Dewi M. Rafi Mahat, Mohd Muzamir Mardiana, Eni Mashuri Waite Maulidiyah Utami Muammar Yulian Nampiah Sukarno Neolaka, Yantus A B Nisa, Ulfiatun NONI NIRWANA Noor Roufiq Ahmadi Novia Rahma NOVIK NOVIK Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novriyandi Hanif Nurdiani Nurdiani Ono Suparno Pius P. KETAREN Purbowatiningrum Ria Sarjono Purwantiningsih Sugita Putra, Sukma Triperdana Rahminiwati, Min Rani, Ismarani RESSY RIANDCI RHOITO FROISTA SILITONGA Rosihan Rosman Rosihan Rosman Rudi Heryanto Rut Novalia Rahmawati Sianipar Sagala, Zuraida Sandra Arifin Aziz Selvitia Ulandari Setyanto Tri Wahyudi Sianipar, Rut Novalia Rahmawati Sri Mulijani Subaryanti Subaryanti Subaryanti Subaryanti Subaryanti Sulitijorini, Sulitijorini SUMINAR SETIATI ACHMADI Taopik Ridwan Tatik Khusniati TRESNAWATI PURWADARIA Tuti Hayati Ramdhani Umi Cahyaningsih Upik Kesumawati Hadi UTUT WIDYASTUTI Waite, Mashuri Wasmen Manalu Wei Zhang Wendi Nurul Fadillah Wiwin Winarsih Wulan Tri Wahyuni YANTYATI WIDYASTUTI Yohana C. Sulistyaningsih Yohana Caecilia Sulistyaningsih Yulia Yellita YULIN LESTARI Zulti, Fifia