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Dyah Iswantini
Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Kampus IPB Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Veterinary

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Journal : Biology, Medicine,

 1 
Superoxide Anion Radicals Biosensor Based on Protein Extract from Deinococcus Radiodurans Immobilization by Glutaraldehyde Cross-Linked Afifi, Muhammad Ridho; Iswantini, Dyah; Nurhidayat, Novik; Saprudin, Deden
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.153-160

Abstract

A common enzyme for superoxide anion radicals biosensors is superoxide dismutase (SOD). Deinococcus radiodurans protein extract can replace the use of pure SOD enzymes for superoxide anion radicals biosensors. The purpose of this research is to determine the analytical performance of the superoxide anion radicals biosensor with protein extract of Deinococcus radiodurans immobilized on carboxymethylcellulose-gelatin-zeolit (CMC-G-Z) that is crosslinked by glutaraldehyde. The response surface method showed that the optimum condition for the biosensor was pH 7, protein extract concentration of 1075 µg/ml, zeolite concentration of 5 mg/ml, and glutaraldehyde concentration of 0.0042 M. Stability of the biosensor retained 67% of its sensitivity after use for 24 hours. The biosensor exhibits good analytical performance with a linear range from 0.1 – 0.8 mM, a detection limit of 77.84 µM, and a limit of quantification of 259.5 µM with a correlation coefficient of 0.9905.
Co-Authors , Triadiati . TRIVADILA Adayani Nurjayanti Adelia, Nesha Adi Riyadhi Adiarso Afifi, Muhammad Ridho Agus Kartono Agus Sudiman Tjokrowardojo, Agus Sudiman Ahmad Sjahriza Ahmad, Shahrul Nizam Ai Susanti Akhiruddin Maddu Akmalina, Rifkah Alfiona, Della Ali Aulia Ghozali Ali Aulia Ghozali Alif, Alfiah Amalyah Febryanti Amalyah Febryanti Ana Fitriyani Anas Miftah Fauzi Anggia Murni Aninda, Yunita Anja Meryandini Bagaskoro Tuwalaid Budi Riza Putra Charlena Deden Saprudin Dewi Sondari Diah Ratnadewi Dian Siska RF Dinda Iryawati Dita Ariyanti DJUMALI MANGUNWIDJAJA Djumali Mangunwidjaja Dwiastuti, Rahma Eka Mardiah ELIZABETH MARTATILOFA Eti Rohaeti Euis Filaila Evul Winoto Evul Winoto Fadillah, Wendi Nurul Firdaus, Ridwan Fitriani Indahsari Franco, Christopher MM Fretty Yurike Fyrda Shellia Ghozali, Ali Aulia Hafshah, Nurul Haki, Horiana hanifah hanifah Harry Noviardi Hasim - Hefni Effendi Henny Purwaningsih Ida Ayu Putu Sri Widnyani Irmanida Batubara Ismet, Rhahmasari Johnson N Naat Joni Prasetyo, Joni Jovianto, Andrian Karnadi Komar Sutriah Kurniawan, Yehezkiel Steven Latifah K Darusman latifah K Darusman Latifah K. Darusman LATIFAH KOSIM DARUSMAN LATIFAH KOSIM DARUSMAN Lawa, Yosep Lenni Fitri Lenni Fitri Lestarani, Dewi M. Rafi Mahat, Mohd Muzamir Mardiana, Eni Mashuri Waite Maulidiyah Utami Muammar Yulian Nampiah Sukarno Neolaka, Yantus A B Nisa, Ulfiatun NONI NIRWANA Noor Roufiq Ahmadi Novia Rahma NOVIK NOVIK Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Novriyandi Hanif Nurdiani Nurdiani Ono Suparno Pius P. KETAREN Purbowatiningrum Ria Sarjono Purwantiningsih Sugita Putra, Sukma Triperdana Rahminiwati, Min Rani, Ismarani RESSY RIANDCI RHOITO FROISTA SILITONGA Rosihan Rosman Rosihan Rosman Rudi Heryanto Rut Novalia Rahmawati Sianipar Sagala, Zuraida Sandra Arifin Aziz Selvitia Ulandari Setyanto Tri Wahyudi Sianipar, Rut Novalia Rahmawati Sri Mulijani Subaryanti Subaryanti Subaryanti Subaryanti Subaryanti Sulitijorini, Sulitijorini SUMINAR SETIATI ACHMADI Taopik Ridwan Tatik Khusniati TRESNAWATI PURWADARIA Tuti Hayati Ramdhani Umi Cahyaningsih Upik Kesumawati Hadi UTUT WIDYASTUTI Waite, Mashuri Wasmen Manalu Wei Zhang Wendi Nurul Fadillah Wiwin Winarsih Wulan Tri Wahyuni YANTYATI WIDYASTUTI Yohana C. Sulistyaningsih Yohana Caecilia Sulistyaningsih Yulia Yellita YULIN LESTARI Zulti, Fifia