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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Gadjah Mada International Journal of Business Journal of Tropical Biodiversity and Biotechnology Science and Technology Indonesia Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Riset Akuakultur JSFK (Jurnal Sains Farmasi & Klinis) Jambura Journal of Chemistry Majalah Farmasetika JURNAL KAJIAN VETERINER Narra J Makara Journal of Science Gunung Djati Conference Series Jurnal Kedokteran Hewan Current Biomedicine Policy Brief Pertanian, Kelautan, dan Biosains Tropika Borneo Journal of Pharmacy Berita Biologi

Huda Shalahudin Darusman

IPB University

Author-ID : 2938356

Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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MONYET EKOR PANJANG (Macaca fascicularis) SEBAGAI HEWAN MODEL DAN ETIKA PENGGUNAANNYA Ratni Ernita; Tatang Mitra Setia; Fitriya Nur Annisa Dewi; Huda Shalahudin Darusman
Gunung Djati Conference Series Vol. 6 (2021): Seminar Nasional Biologi (SEMABIO) 6 Tahun 2021
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (376.278 KB)

The use of nonhuman primates as animal models in biomedical research has an important role in the development of human health. A close phylogenetic relationship with humans as well as similarities in genetics, behavior and biochemical activities are some of the things that underlie the selection of nonhuman primates as animal models. Cynomolgus macaques (Macaca fascicularis) is one of Indonesia's endemic primates which is widely used in research. Its current status as Appendix II in Convention International Trade in Endangered Species of Wild Fauna and Flora (CITES) defines that the population remains quite large in nature, which allows this species to be used in research, as long as they are sourced from the captive-bred population. Although the use of nonhuman primates in research is unavoidable, it is warranted that potential harm such as distress, discomfort, stress, pain and even death have to be avoided or minimized. Implementation of ethics is therefore required for the care and use of cynomolgus macaques in research, such as by assuring that the 3R principles (replacement, reduction, refinement) are met. Refinement can be achieved by applying the principles of animal welfare known as the Five Freedoms of Animal Welfare (5F). The active implementation of these ethical principles continues to develop along with the development of science and technology, and receives more spotlight in the world of biomedical research globally. Ensuring adequate implementation of ethical principles in research with nonhuman primates in each research institution must be under the oversight of an IACUC (Institutional Animal Care and Use Committee), which will weigh on the harm to animals compared to the benefits to humans. Active implementation of the principles of ethics and animal welfare will result in increasing the quality of research, serves as an assurance to the society, and in compliance to the requirements for good scientific publication.

Analyses of Vegetation Used by Long-tailed Macaque (Macaca fascicularis Raffles 1821) in Tinjil Island Dyah Perwitasari-Farajallah; Hana Intishar Sawitri; Silvy Thiyana; Tommy Langgeng Abimanyu; Entang Iskandar; Huda Shalahudin Darusman
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 1 (2023): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.70739

Tinjil Island is a semi-natural breeding facility for long-tailed macaques (Macaca fascicularis) managed by Primate Research Center, IPB University, located at the southern of Java Island and surrounded by the Indian Ocean. Long-tailed macaques are considered frugivorous even though they are well-known for their flexible diet. This study aims to analyse the vegetation supporting the population of long-tailed macaques. Data were collected from six tracks using square sampling plots with the size of 20 m x 20 m for trees as the main plot, inside the main plot were square subplots consisting of 10 m x 10 m for poles, 5 m x 5 m for saplings, and 2 m x 2 m for seedlings. The Important Value Index (IVI) was calculated for each level of vegetation. Hanjuang (Dracaena elliptica) dominated the seedlings with 29.35%, followed by Kampis (Hernandia peltata) with 18.73%, and Kalapari (Pongamia pinnata) with 13.73%. Hanjuang (Dracaena elliptica) also dominated the saplings with 26.83%, followed by Pancal (Syzygium antisepticum) with 19.19%, and Laban (Vitex pubescens) with 12.30%. The poles were dominated by Ki Cau (Dolichandrone spathacea) as high as 59.28%, while Waru (Thespesia populnea) and Ki Ciat (Ficus septica) dominated at 40.47% and 36.15%, respectively. Kampis (Hernandia peltata) dominated the trees with 39.28%, followed by Ki Ara (Ficus glomerata) with 35.56%, and Ki Langir (Dysoxylum amooroides) with 28.70%. Species found on Tinjil Island are mostly Moraceae (9.84%) and Fabaceae (9.84%), followed by Malvaceae (8.20%), Euphorbiaceae (4.92%), Myrtaceae (4.92%), and Anacardiaceae (4.92%). The vegetation in Tinjil Island supports the livelihood of long-tailed macaques on the island because they have an abundance of food and staple food such as figs to help them fulfil the energy needed to survive and reproduce.

CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM WHITE ADIPOSE TISSUE OF Macaca fascicularis Harrold Halilintar MR Subu Taopan; Fitriya Nur Annisa Dewi; Silmi Mariya; Huda Darusman; Permanawati Permanawati; Dondin Sajuthi; Irma Herawati Suparto
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Mesenchymal stem cells (MSC) derived from white adipose tissue are considered to be an appropriate therapeutic candidate for various human diseases due to their natural ability to maintain homeostasis. They have also been considered for use as a therapeutic alternative due to their sufficient availability and ease of harvesting. MSC play an important role in the process of adipogenesis and energy metabolism that is directly related to the pathogenesis of various degenerative diseases and metabolic syndromes. The purpose of this study was to obtain MSC cultures from white adipose tissue biopsied from three adult males Macaca fascicularis and to evaluate these cultures qualitatively and quantitatively regarding their characteristics and ability to differentiate. Polymerase chain reaction (PCR) was used to confirm the expression of mesenchymal stem cell-specific gene markers, and a specific growth medium was used to differentiate these cells into adipocytes, chondrocytes, and osteocytes. The results obtained showed that the MSC culture from M. fascicularis WAT had a fibroblast-like morphology with a spindle shape. The MSC markers of CD73, CD90 and CD105, were also expressed. The MSC culture was also able to differentiate into adipocytes, chondrocytes, and osteocytes. Based on these results, white adipose tissue from Macaca fascicularis was found to have potential as a source of MSC.

Expression of APP, CDK5, and AKT1 Gene Related to Alzheimer Disease in Brain of Long-tailed Macaques Lis Rosmanah; Uus Saepuloh; Sela Septima Mariya; Irma Herawati Suparto; Wasmen Manalu; Adi Winarto; Huda Shalahudin Darusman
HAYATI Journal of Biosciences Vol. 31 No. 1 (2024): January 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.1.145-152

Amyloid plaques and Neurofibrillary Tangles (NFTs) are known to be key pathological features of Alzheimer disease. To gain a better understanding of this disease, studies were carried out on the Indonesian primates, the long-tailed macaques, using a spontaneous Alzheimer's disease model. Examining and identifying genetic markers involved in plaque formation and NFTs in long-tailed macaques is necessary to reveal their physiological processes. In this study, the expression of genes involved in the development of amyloid plaque (Amyloid Precursor Protein (APP)) and those that control the phosphorylation of tau protein (CDK5 and AKT1) was examined in the long-tailed macaque brain. This study showed that APP, CDK5, and AKT1 may potentially be developed as genetic markers of Alzheimer's disease. Long-tailed macaques exhibited the development of amyloid plaque in the aging brain based on the analysis of the gene expression profile of its biomarker. Furthermore, long-tailed macaques can be optimized for neurodegenerative models.

Development and Optimization of an Immunoassay for the Detection of Antibodies Against SARS-CoV-2 with In-house Recombinant RBD Protein Ratu, Safira Pinaka Pramestika; Mariya, Silmi; Noviana, Rachmitasari; Saepuloh, Uus; Darusman, Huda Salahudin
Makara Journal of Science Vol. 26, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

COVID-19 caused by SARS-CoV-2 poses a major threat to the global community, particularly in Indonesia. Countermeasures to prevent the spread of this disease have also been implemented, including the implementation of a vaccination program. An immunoassay technique that can be used to analyze antibodies that might develop following vaccination is the indirect enzyme-linked immunosorbent assay (ELISA). We produced the recombinant spike protein used in this study. The optimization comprised adjusted concentrations of spike recombinant protein (5 and 10 ng/mL), blocking agent (2.5% and 5%), and conjugate (1:1000 and 1:5000). The optimal conditions in this study included a spiked concentration of 10 ng/mL, a blocking agent concentration of 5%, sample dilution of 1:33, and a conjugate concentration of 1:1000. The intra-assay value of this optimized indirect ELISA was 7.3, and the inter-assay value was 5.3. The commercial MyBioSource kit and immunodiagnostic were utilized as a reference in the T-test, with P-values of 0 and 0.313, indicating that the recombinant protein in-house ELISA kit in this study demonstrated the same ability as the commercial immunodiagnostic kit in detecting SARS-CoV-2 antibodies, allowing it to be used for post-vaccination efficacy evaluation.

Kejadian nematodosis gastrointestinal pada monyet ekor panjang (Macaca fascicularis) di Pusat Studi Satwa Primata LPPM IPB Rosyid, Bahaudien; Darusman, Huda Shalahudin; Retnani, Elok Budi
Current Biomedicine Vol. 1 No. 1 (2023): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.1.1.33-45

Background: The long-tailed macaque (Macaca fascicularis) is often used as a model animal in biomedical research. Nematodosis is common among non-human primates. Gastrointestinal nematodosis may affect study results or be a potential source of transmission to humans (zoonosis). Objective: The study aimed to identify the type, calculate the degree of infection, and the prevalence of gastrointestinal nematode worms in long-tailed macaques in captivity at the Primate Research Center, Institute for Research and Community Service (PSSP LPPM) IPB University. Methods: Faeces samples from 24 long-tailed macaques were examined using the McMaster and Kato-Katz simple flotation methods. Results: Nine (37.5%) of the 24 samples were infected by trichurid (20.8%), ascarid (12.5%), and strongylid (4.2%), each with an average degree of infection 833.3 ± 923.8, 32 ± 27.7, and 12 ± 17 eggs per gram of faeces (EPG), respectively. The prevalence of nematodosis in long-tailed macaques based on age group and sex was in infants 50%, children 50%, adolescents 16.7%, adults 33.3%, males 33.3%, and females 41.7%. Conclusion: The incidence and degree of gastrointestinal nematodosis infection in long-tailed macaques showed a moderate to low level. These results can be considered in maintenance management, biomedical research, and mitigation of potential transmission risk to humans.

Perilaku Sepasang Cephalopachus bancanus di Kandang Konservasi Pusat Studi Satwa Primata, Bogor Wira, Dwi Wahyuda; Fadla, Ilma Nadya; Hiroyuki, Andi; Mayasari, Novi; Iskandar, Entang; Darusman, Huda Salahudin; Farajallah, Dyah Perwitasari
Acta VETERINARIA Indonesiana Vol. 12 No. 1 (2024): Maret 2024
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.12.1.55-64

Cephalopachus bancanus saat ini dikategorikan sebagai hewan rentan (vulnerable) oleh International Union for Conservation of Nature (IUCN). Penurunan populasi ini terjadi karena hilangnya habitat dan tingkat eksploitasi yang tinggi. Pemahaman perilaku Cephalopachus bancanus dalam konservasi dapat digunakan untuk menunjang perkembangbiakan satwa dalam kaitan mendukung upaya konservasi agar satwa endemik ini tidak menurun populasinya. Penelitian ini bertujuan untuk mengukur frekuensi perilaku sepasang Cephalopachus bancanus guna mendapatkan data dan informasi mengenai perilaku sepasang dalam kandang konservasi Pusat Studi Satwa Primata, Bogor. Penelitian perilaku pasangan yang teramati diantara lain: perilaku berkelompok, perilaku saling membersihkan diri, perilaku bertengkar, berselisih dan menghindar, perilaku bermain, perilaku seksual. Metode pengamatan yang digunakan adalah focal animal sampling dan instantaneous sampling dengan penilaian one-zerotime sampling. Hasil pengamatan selama 5 periode pengulangan waktu menunjukkan bahwa perilaku berkelompok mempunyai frekuensi paling tinggi yaitu 40% dengan durasi 134 menit, perilaku saling membersihkan diri menunjukkan frekuensi 37% dengan durasi 121 menit, perilaku bertengkar, berselisih dan menghindar menunjukkan frekuensi 18% dengan durasi 61 menit, perilaku bermain menunjukkan frekuensi 4% dengan durasi 12 menit, dan perilaku seksual menunjukkan hasil frekuensi paling rendah, yaitu 1% dengan durasi 4 menit. Kesimpulan dari penelitian ini yaitu perilaku sosial sepasang Cephalopachus bancanus paling banyak dilakukan yaitu perilaku berkelompok dan paling sedikit dilakukan yaitu perilaku seksual.

Myristicin Inhibit Invasion and Migration of Melanoma Cells through Suppression of MMP2 and MMP9 Gene Expression Krisnayanti, Ni Putu Eka; Mariya, Silmi; Indriawati, Iin; Dayana, Hepy; Zaelani, Bella Fatima Dora; Tjandrawinata, Raymond R.; Darusman, Huda Shalahudin
Science and Technology Indonesia Vol. 9 No. 4 (2024): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.4.884-892

Melanoma is the deadliest type of skin cancer, having a high mortality rate. This cancer has an aggressive nature, is highly invasive, and has the tendency to metastasize. Matrix metalloproteinases (MMPs) are essential in that process, especially MMP2 and MMP9. Their expression is upregulated during metastasis progression. Myristicin is one example of a compound that can be utilized to target MMP 2 and MMP 9 in melanoma. This research concerns the activity of myristicin to inhibit melanoma cell invasion and migration by suppressing MMP2 and MMP9 gene expression. The MTT assay in this study demonstrated that myristicin exhibited strong cytotoxic activity against melanoma cells. This compound works in a dose-dependent manner by inhibiting cell migration and invasion. The invasion test was performed using the transwell assay, whereas the migration test was performed using the wound healing assay. The invasion assay results were consistent with MMP2 and MMP9 gene expression. These two genes were analyzed using the RT-qPCR technique. It has been demonstrated that low gene expression in melanoma cells inhibits cell invasion. In contrast, higher MMP2 and MMP9 gene expression was associated with an increase in the number of invasive cells on average. However, MMP2 and MMP9 in excessive expression and uncontrolled activity impair the ability of melanoma cells to form a monolayer sheet to cover wound gaps. This condition significantly reduced the migration rate and percentage of wound closure.

3D Culture Cells Technique for Exosomes Isolation of HEK293 and its Application on WiDr Cells Audina, Mia; Mariya, Silmi; Zaelani, Bella Fatima Dora; Yuliana; Darusman, Huda Shalahudin
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1173-1180

Three-dimensional (3D) culture is a technique commonly utilized in bioprocessing and biomedical research. Exosomes have been investigated as carriers for medications in numerous studies employing 3D culture methodologies. The objective of this research is to employ 3D cell culture for the isolation and treatment of exosomes targeting colon cancer cells. The isolation of exosomes obtained from HEK293 cells was conducted through the ultracentrifugation technique. Subsequently, exosome treatment was administered to WiDr cells at concentrations of 3.5 µg/ml, 7 µg/ml, and 14 µg/ml.The validation of molecular markers of exosomes (CD9 and CD81), along with BAX, BCL-2, and CD133, was performed using qRT-PCR. The findings revealed the successful isolation of exosomes derived from HEK293 cells, which exhibited the expression of markers CD9 and CD81. Furthermore, the expression of BAX and BCL-2 indicated the potential of exosomes to induce apoptosis, while the expression level of CD133 decreased with treatment at varying concentrations. These results suggest that exosome treatment has the capability to impede the proliferation of WiDr cells and reduce the expression of CD133, thereby signifying the potential application of exosomes as an in-vitro model for investigating cancer therapy in the future.

Cloning, Expression, and Bioinformatics Modeling of Human Papillomavirus Type 52 L1/L2 Chimeric Protein in Escherichia coli BL21 (DE3) Ikramullah, Muh. Chaeril; Mustopa, Apon Zaenal; Wibawa, Tri; Hertati, Ai; Umami, Rifqiyah Nur; Ratna, Lita Tri; Irawan, Shasmita; Firdaus, Moh Egy Rahman; Darusman, Huda Salahudin
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.891-902

Human papillomavirus (HPV) L1 major capsid protein generates a highly immunogenic virus like particles (VLPs), which have been used as the main component of its prophylactic vaccine. However, the neutralizing antibodies against L1 VLPs are mostly type specific and may not be effective to prevent infection from different strains of HPV. On the other hand, HPV L2 minor capsid protein has low antigenic variation, thus can induce cross-neutralization. This study aims to obtain HPV 52 L1/L2 chimeric protein, which is designed based on HPV type 52 as one of the most circulated high-risk types in Indonesia, to develop a broad-spectrum HPV vaccine. Substitution of HPV 52 H4 helix L1 region with an HPV 52 L2 epitope was carried out using overlap extension PCR. HPV 52 L1/L2 chimeric gene was constructed into pET-SUMO expression vector and expressed in Escherichia coli BL21 (DE3). Bioinformatics modeling suggested that L2 epitope was located inside of the loop region in monomer form, and on the contrary, it was located outside of the pentamer surface. Furthermore, B cell and T cell epitopes predictions were conducted using Immune Epitope Database (IEDB) analysis. The B cell epitopes prediction revealed eleven potential epitopes, whereas the T cell epitopes prediction showed seven potential epitopes for each MHC class I and MHC class II. This study showed that HPV 52 L1/L2 chimeric protein has the potential to induce cross-neutralizing antibodies and can be developed as a promising candidate for a new HPV vaccine.

Co-Authors A.S. Satyaningtijas Achmad, Yusuf Farid Adi Winarto Adi Winarto Agik Suprayogi Ahmad Kamal Nasution Ahmad Subhan Ai Hertati, Ai Akhiruddin Maddu Andhika Yudha Prawira Andi Hiroyuki Angela Mariana Lusiastuti Anita Esfandiari Annisa Dewi, Fitriya Nur Apon Zaenal Mustopa Aprilyana, Lia Ardiansyah Nurdin Benvika Damiana Rita Ekastuti Dayana, Hepy Dewi Ratih Agungpriyono Dewi, Fitriya Nur Annisa Dondin Sajuthi DYAH PERWITASARI -FARAJALLAH Ekowati Handharyani Elok Budi Retnani Entang Iskandar Entang Iskandar Entang Iskandar Entang Iskandar Ernes Andhesfa Etih Sudarnika Fadla, Ilma Nadya Fauzi Abdurrahman Munggaran Femke Den Haas Ferdinandez, Mariana Fiero, Nur Rizky Firdaus, Moh Egy Rahman Fitri, Arni Diana Habib Ilham Maulana Al Adhim Hana Intishar Sawitri Hardo Basuki Harrold Halilintar MR Subu Taopan Hasya, Karin katina HERA MAHESHWARI Hidayaturrahmah Hidayaturrahmah I wayan Teguh Wibawan Ice Lusia Marta IETJE WIENTARSIH Ikramullah, Muh. Chaeril Indra Bachtiar Indra Bachtiar Indriawati, Iin Iqbal Ngabdusani Irawan, Shasmita Irma Herawati Suparto Joko Pamungkas Jusak Nugraha Kanti Rahmi Fauziyah Katrin Roosita koekoeh santoso Krisnayanti, Ni Putu Eka Lina Elfita Lina Elfita Linda Tjhin Lis Rosmanah Loe, Fhady Risckhy Loes (ML) Schure Maria Fatima Palupi Maria Fatima Palupi Maria Fatima Palupi Megawati Iskandar Mia Audina Muhammad Hamdan Mustopa, Apon Z. Ningrum, Emilna Mega Novi Mayasari Noviana, Rachmitasari Novriyandi Hanif Nugroho, Setyo Widi Perdhana, Ika Satya Permanawati Permanawati PERMANAWATI, PERMANAWATI Puji Rianti Putri, Indah Aprianti Rachmitasari Noviana Rahmania Hanim Rahminiwati, Min Randall C. Kyes Rasyida Ulfa Ratna, Lita Tri Ratni Ernita Ratu, Safira Pinaka Pramestika Raymond R. Tjandrawinata Ridzki M.F. Binol Rifqiyah Nur Umami, Rifqiyah Nur Rika Sudranto Rizal, Muhammad Farid Rizki Aulia Ansari Roostita L Balia Rosmanah, Lis Rosyid, Bahaudien Sari, Isti Kartika Savitri Novelina Sela Septima Mariya Setyawaty, Dyah Setyo Widi Nugroho Sibit, Denniswara Silmi Mariya SILMI MARIYA Silmi Mariya Silvy Thiyana Soedarso, Yovi Alwi Sony Hartono Wijaya Sri Wahyuni Srihadi Agungpriyono Subu Taopan, Harrold Halilintar MR Suduri, Adam Sukenda Sukenda Sumitro - Sundari, Betty Suparto, Irma H. SURYA, MARYATI Suryo Saputro Tambunan, Anggitha Tatang Mitra Setia Tatik Mufidah Tommy Langgeng Abimanyu Tri Wibawa Uus Saepuloh UUS SAEPULOH Uus Saepuloh Vinka Aftinata Kusumaputri Wartika Rosa Farida Wasmen Manalu WIDANARNI WIDANARNI Wira, Dwi Wahyuda Yuliana Yuliana Yuliana Zaelani, Bella Fatima Dora

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