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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Gadjah Mada International Journal of Business Journal of Tropical Biodiversity and Biotechnology Science and Technology Indonesia Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Riset Akuakultur JSFK (Jurnal Sains Farmasi & Klinis) Jambura Journal of Chemistry Majalah Farmasetika Narra J Makara Journal of Science Gunung Djati Conference Series Jurnal Kedokteran Hewan Current Biomedicine Policy Brief Pertanian, Kelautan, dan Biosains Tropika Borneo Journal of Pharmacy Berita Biologi
Huda Shalahudin Darusman
IPB University
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CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM WHITE ADIPOSE TISSUE OF Macaca fascicularis Subu Taopan, Harrold Halilintar MR; Annisa Dewi, Fitriya Nur; Mariya, Silmi; Darusman, Huda; Permanawati, Permanawati; Sajuthi, Dondin; Suparto, Irma Herawati
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i4.18203

Abstract

Mesenchymal stem cells (MSC) derived from white adipose tissue are considered to be an appropriate therapeutic candidate for various human diseases due to their natural ability to maintain homeostasis. They have also been considered for use as a therapeutic alternative due to their sufficient availability and ease of harvesting. MSC play an important role in the process of adipogenesis and energy metabolism that is directly related to the pathogenesis of various degenerative diseases and metabolic syndromes. The purpose of this study was to obtain MSC cultures from white adipose tissue biopsied from three adult males Macaca fascicularis and to evaluate these cultures qualitatively and quantitatively regarding their characteristics and ability to differentiate. Polymerase chain reaction (PCR) was used to confirm the expression of mesenchymal stem cell-specific gene markers, and a specific growth medium was used to differentiate these cells into adipocytes, chondrocytes, and osteocytes. The results obtained showed that the MSC culture from M. fascicularis WAT had a fibroblast-like morphology with a spindle shape. The MSC markers of CD73, CD90 and CD105, were also expressed. The MSC culture was also able to differentiate into adipocytes, chondrocytes, and osteocytes. Based on these results, white adipose tissue from Macaca fascicularis was found to have potential as a source of MSC.
PENAPISAN MODEL HEWAN PRIMATA Macaca fascicularis UNTUK UJI VAKSIN Human Papilloma Virus Sari, Isti Kartika; Mariya, Silmi; Setyawaty, Dyah; Suparto, Irma H.; Mustopa, Apon Z.; Darusman, Huda Salahudin
Berita Biologi Vol 23 No 2 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.1052

Abstract

Pemanfaatan hewan primata dalam hal ini monyet ekor panjang (MEP) atau Macaca fascicularis sebagai hewan model untuk uji vaksin Human Papilloma Virus (HPV) harus melalui penapisan untuk mendapatkan hewan yang tepat. Tujuan dari penelitian ini untuk menapis dan memvalidasi kecocokan MEP sebagai hewan model vaksin papillomavirus. Jenis vaksin yang akan di uji menentukan jenis penapisan yang dilakukan. Metode penapisan dilakukan dengan uji serologis, molecular dan patologi serta validasi dengan histopatologi. Vaksin pencegahan atau profilaksis memerlukan hewan yang bebas dari virus-virus penekan kekebalan tubuh (immunosuppressive virus) dan HPV nya sendiri agar antibodi yang terbentuk benar-benar berasal dari vaksin yang diujikan, vaksin terapeutik memilih hewan dengan positif MfPV khususnya MfPV3. Hasil penapisan dari 136 ekor hewan diperoleh 42 (30%) ekor hewan dengan negatif antibodi serta  diperoleh  39 (28%) ekor MEP positif MfPV diantaranya yaitu sebanyak 24 (17%) ekor positif MfPV-3. Hewan dengan positif MfPV3 selanjutnya akan digunakan untuk uji vaksin terapeutik HPV. Adanya perubahan abnormal sel-sel epitel serviks pada hewan dengan positif MfPV yang menuju pada pembentukkan sel kanker (Carcinoma Intra epithelial Neoplasia/CIN) juga semakin menguatkan kemiripan model MEP dengan manusia.
Hematological Profile of Macaca nemestrina Across Different Age Groups Sri Wahyuni; Fitriya Nur Annisa Dewi; Anita Esfandiari; Suryo Saputro; Huda Shalahudin Darusman
Acta VETERINARIA Indonesiana Vol. 13 No. 3 (2025): November 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.3.217-223

Abstract

Macaca nemestrina, also known as the pigtail macaque, plays a crucial role in drug development and serves as a highly valuable animal model for studying various human diseases due to its phenotypic similarities to humans, including those related to the aging process. Hematology is a critical component of clinical assessments for this spesies, serving both as a diagnostic tool to determine their health status and to characterize disease models, including degenerative and geriatric diseases. As anemia is a common phenomenon in the elderly, it is also important to determine whether M. nemestrina exhibit a similar condition with age. The aim of this study was to determine the hematological profiles of clinically healthy female M. nemestrina across young, adult, and elderly age groups, particularly related to anemia parameters. This study was conducted at the Primate Research Center, IPB University, Bogor, Indonesia. The animals were part of a breeding colony and were divided into three groups based on age: young (5–7 years), adult (12–14 years), and elderly (18–21 years). Following anesthesia with ketamine, a physical examination was performed to determine clinical status, and blood samples were collected for a complete blood count (CBC) evaluation using an automated analyzer. The results showed that total erythrocytes, hemoglobin concentration, hematocrit value, and mean corpuscular hemoglobin (MCH) were significantly different (P<0.05) in the elderly group compared to the other groups. Additionally, the red cell distribution width (RDW) percentage and leukocyte count differed significantly between the adult and elderly groups. The results showed that the erythrogram differs with age in clinically healthy M. nemestrina, suggesting the importance of determining appropriate baseline reference values in macaques of different ages.
Preclinical Evaluation of HPV Type 52 L1L2 Chimeric Protein as a Cervical Cancer Vaccine Candidate Sari, Isti Kartika; Pamungkas, Joko; Mustopa, Apon Zaenal; Wibawan, I Wayan Teguh; Mamangkey, Jendri; Chairunnisa, Sheila; Irawan, Herman; Hertati, Ai; Ekawati, Nurlaili; Umami, Rifqiyah Nur; Novianti, Ela; Nurfatwa, Maritsa; Darusman, Huda Shalahudin
HAYATI Journal of Biosciences Vol. 33 No. 3 (2026): May 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.3.556-565

Abstract

High-risk human papillomaviruses (HPVs) are the primary etiological agents of cervical cancer, accounting for more than 300,000 deaths annually worldwide. Current prophylactic vaccines based on recombinant L1 major capsid virus-like particles (VLPs) have demonstrated strong efficacy but are restricted to a limited spectrum of HPV types. To address this limitation, the present study evaluated a recombinant L1L2 chimeric protein derived from HPV type 52 as a potential candidate for a broad-spectrum vaccine. The chimeric protein was expressed in Escherichia coli strain BL21 (DE3) and purified for immunization studies. Female BALB/c mice (Mus musculus, n = 5 groups) were immunized, and immune responses were analyzed by enzyme-linked immunosorbent assay (ELISA) and pseudovirion-based neutralization assays (PBNA). The recombinant L1L2 vaccine candidate induced detectable antibody responses against HPV antigens; however, neutralizing activity remained modest. Histopathological analysis of liver and kidney tissues showed no evidence of toxicity, supporting the safety profile of the candidate. In summary, these results suggest that the HPV type 52 L1L2 chimeric protein represents a promising platform for the development of cervical cancer vaccines, although further optimization is required to achieve robust cross-neutralizing efficacy.
Standarisasi Penjaminan Kesejahteraan Hewan Untuk Kemajuan Riset Biomedis Fitriya Nur Annisa Dewi; Huda Shalahudin Darusman; Joko Pamungkas
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol. 4 No. 4 (2022): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0404.375-380

Abstract

Pengembangan berbagai obat, vaksin dan produk kesehatan memerlukan tahap penelitian dan pengujian pada hewan laboratorium/hewan coba. Kesejahteraan hewan selama pemeliharan dan penggunaannya dalam studi merupakan salah satu faktor penting yang menentukan kesahihan hasil, dan pemenuhannya semakin menjadi sorotan publik dan tuntutan global. Diperlukan aturan hukum, kebijakan dan pedoman nasional untuk menjamin implementasi prinsip kesejahteraan hewan yang terstandar di berbagai institusi di Indonesia, dan mengacu pada standar internasional. Kajian ini bertujuan menelaah aspek legal di Indonesia yang mengatur implementasi prinsip etik dan kesejahteraan hewan di bidang penelitian biomedis, dan mengidentifikasi kebijakan dan pedoman yang diperlukan untuk mencapai standarisasi penjaminan tersebut.
Co-Authors A.S. Satyaningtijas Adi Winarto Adi Winarto Agik Suprayogi Ahmad Kamal Nasution Ahmad Subhan Ai Hertati, Ai Akhiruddin Maddu Andhika Yudha Prawira Andi Hiroyuki Angela Mariana Lusiastuti Anita Esfandiari Annisa Dewi, Fitriya Nur Apon Zaenal Mustopa Aprilyana, Lia Ardiansyah Nurdin Benvika Chairunnisa, Sheila Damiana Rita Ekastuti Dayana, Hepy Dewi Ratih Agungpriyono Dewi, Fitriya Nur Annisa Dondin Sajuthi DYAH PERWITASARI -FARAJALLAH Ekawati, Nurlaili Ekowati Handharyani Elok Budi Retnani Entang Iskandar Entang Iskandar Entang Iskandar Entang Iskandar Ernes Andhesfa Etih Sudarnika Fadla, Ilma Nadya Fauzi Abdurrahman Munggaran Femke Den Haas Ferdinandez, Mariana Firdaus, Moh Egy Rahman Fitri, Arni Diana Habib Ilham Maulana Al Adhim Hana Intishar Sawitri Hardo Basuki Harrold Halilintar MR Subu Taopan Hasya, Karin katina HERA MAHESHWARI Hidayaturrahmah Hidayaturrahmah I wayan Teguh Wibawan Ice Lusia Marta IETJE WIENTARSIH Ikramullah, Muh. Chaeril Indra Bachtiar Indra Bachtiar Indriawati, Iin Iqbal Ngabdusani Irawan, Herman Irawan, Shasmita Irma Herawati Suparto Jendri Mamangkey Joko Pamungkas Jusak Nugraha Kanti Rahmi Fauziyah Katrin Roosita koekoeh santoso Krisnayanti, Ni Putu Eka Lina Elfita Lina Elfita Linda Tjhin Lis Rosmanah Loes (ML) Schure Maria Fatima Palupi Maria Fatima Palupi Megawati Iskandar Mia Audina Muhammad Hamdan Mustopa, Apon Z. Ningrum, Emilna Mega Noviana, Rachmitasari Novianti, Ela Novriyandi Hanif Nugroho, Setyo Widi Nurfatwa, Maritsa Pamungkas, Joko Perdhana, Ika Satya Permanawati Permanawati PERMANAWATI, PERMANAWATI Puji Rianti Putri, Indah Aprianti Rachmitasari Noviana Rahmania Hanim Rahminiwati, Min Randall C. Kyes Rasyida Ulfa Ratna, Lita Tri Ratni Ernita Ratu, Safira Pinaka Pramestika Raymond R. Tjandrawinata Ridzki M.F. Binol Rifqiyah Nur Umami, Rifqiyah Nur Rika Sudranto Rizki Aulia Ansari Rosmanah, Lis Rosyid, Bahaudien Sari, Isti Kartika Savitri Novelina Sela Septima Mariya Setyawaty, Dyah Setyo Widi Nugroho Silmi Mariya SILMI MARIYA Silmi Mariya Silvy Thiyana Sony Hartono Wijaya Sri Wahyuni Srihadi Agungpriyono Subu Taopan, Harrold Halilintar MR Suduri, Adam Sukenda Sukenda Sumitro - Suparto, Irma H. Suryo Saputro Tambunan, Anggitha Tatang Mitra Setia Tatik Mufidah Tommy Langgeng Abimanyu Tri Wibawa Uus Saepuloh UUS SAEPULOH Uus Saepuloh Vinka Aftinata Kusumaputri Wartika Rosa Farida Wasmen Manalu WIDANARNI WIDANARNI Wira, Dwi Wahyuda Yuliana Yuliana Yuliana Zaelani, Bella Fatima Dora