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Huda Shalahudin Darusman
IPB University
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THE GENE EXPRESSION OF ADAM17 AS A GENETIC MARKER OF ALZHEIMER DISEASES IN THE BRAIN OF LONG-TAILED MACAQUES (Macaca fascicularis) Rosmanah, Lis; Hasya, Karin katina; Saepuloh, Uus; Manalu, Wasmen; Winarto, Adi; Sudarnika, Etih; Darusman, Huda Shalahudin
Jurnal Kedokteran Hewan Vol 17, No 3 (2023): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i3.31014

Abstract

This study aims to identify the expression of the a disintegrin and metalloproteinase 17 (ADAM17) gene as a marker of Alzheimer's disease in long-tailed macaques (Macaca fascicularis). This study used six brain samples (hippocampus and cortex regions) of long-tailed macaques which was divided into two groups consisting of aged long-tailed macaques and adult long-tailed macaques. The expression of ADAM17 gene was determined by comparing the relative quantification values between the two age groups, and brain regions consisting of the hippocampus and cortex regions. The results of data analysis showed no significant difference in the expression of ADAM17 gene between brain regions and between age groups of long-tailed macaques. However, numerically the results showed a lower expression of ADAM17 gene in the hippocampus region of aged macaques. Lower expression of ADAM17 gene could be a marker of old animals indicating the stages of Alzheimer's disease.
CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM WHITE ADIPOSE TISSUE OF Macaca fascicularis Subu Taopan, Harrold Halilintar MR; Annisa Dewi, Fitriya Nur; Mariya, Silmi; Darusman, Huda; Permanawati, Permanawati; Sajuthi, Dondin; Suparto, Irma Herawati
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i4.18203

Abstract

Mesenchymal stem cells (MSC) derived from white adipose tissue are considered to be an appropriate therapeutic candidate for various human diseases due to their natural ability to maintain homeostasis. They have also been considered for use as a therapeutic alternative due to their sufficient availability and ease of harvesting. MSC play an important role in the process of adipogenesis and energy metabolism that is directly related to the pathogenesis of various degenerative diseases and metabolic syndromes. The purpose of this study was to obtain MSC cultures from white adipose tissue biopsied from three adult males Macaca fascicularis and to evaluate these cultures qualitatively and quantitatively regarding their characteristics and ability to differentiate. Polymerase chain reaction (PCR) was used to confirm the expression of mesenchymal stem cell-specific gene markers, and a specific growth medium was used to differentiate these cells into adipocytes, chondrocytes, and osteocytes. The results obtained showed that the MSC culture from M. fascicularis WAT had a fibroblast-like morphology with a spindle shape. The MSC markers of CD73, CD90 and CD105, were also expressed. The MSC culture was also able to differentiate into adipocytes, chondrocytes, and osteocytes. Based on these results, white adipose tissue from Macaca fascicularis was found to have potential as a source of MSC.
PENAPISAN MODEL HEWAN PRIMATA Macaca fascicularis UNTUK UJI VAKSIN Human Papilloma Virus Sari, Isti Kartika; Mariya, Silmi; Setyawaty, Dyah; Suparto, Irma H.; Mustopa, Apon Z.; Darusman, Huda Salahudin
Berita Biologi Vol 23 No 2 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.1052

Abstract

Pemanfaatan hewan primata dalam hal ini monyet ekor panjang (MEP) atau Macaca fascicularis sebagai hewan model untuk uji vaksin Human Papilloma Virus (HPV) harus melalui penapisan untuk mendapatkan hewan yang tepat. Tujuan dari penelitian ini untuk menapis dan memvalidasi kecocokan MEP sebagai hewan model vaksin papillomavirus. Jenis vaksin yang akan di uji menentukan jenis penapisan yang dilakukan. Metode penapisan dilakukan dengan uji serologis, molecular dan patologi serta validasi dengan histopatologi. Vaksin pencegahan atau profilaksis memerlukan hewan yang bebas dari virus-virus penekan kekebalan tubuh (immunosuppressive virus) dan HPV nya sendiri agar antibodi yang terbentuk benar-benar berasal dari vaksin yang diujikan, vaksin terapeutik memilih hewan dengan positif MfPV khususnya MfPV3. Hasil penapisan dari 136 ekor hewan diperoleh 42 (30%) ekor hewan dengan negatif antibodi serta  diperoleh  39 (28%) ekor MEP positif MfPV diantaranya yaitu sebanyak 24 (17%) ekor positif MfPV-3. Hewan dengan positif MfPV3 selanjutnya akan digunakan untuk uji vaksin terapeutik HPV. Adanya perubahan abnormal sel-sel epitel serviks pada hewan dengan positif MfPV yang menuju pada pembentukkan sel kanker (Carcinoma Intra epithelial Neoplasia/CIN) juga semakin menguatkan kemiripan model MEP dengan manusia.
Characterisation of E. coli Virulence Factors Causing Diarrhea in Macaca Fascicularis at IPB University Captivity, Bogor, Jawa Barat, Indonesia Rizal, Muhammad Farid; Wibawan, I Wayan Teguh; Pamungkas, Joko; Balia, Roostita L.; Darusman, Huda Shalahudin; Surya, Maryati; Achmad, Yusuf Farid; Sundari, Betty; Soedarso, Yovi Alwi; Sibit, Denniswara; Loe, Fhady Risckhy
JURNAL KAJIAN VETERINER Vol 13 No 2 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i2.20778

Abstract

Diare pada Macaca fascicularis sangat mengganggu proses penangkaran, diare mengurangi kualitas hidup kera, E. coli sering dilaporkan sebagai penyebab utama diare pada beberapa primata non-manusia. Tujuan dari penelitian ini adalah untuk mendeteksi faktor virulensi seperti hemolisin, pola hemaglutinin, dan hidrofobisitas permukaan sel. Penelitian ini menggunakan 30 isolat E. coli dari koleksi pusat penelitian primata IPB University yang diisolasi dari kera ekor panjang yang menderita diare. Faktor virulensi seperti hemolisin, hemaglutinasi, dan agregasi garam dideteksi sesuai protokol standar. Sebanyak 30 isolat M. fascicularis yang menderita diare diuji kemampuannya menyebabkan lisis pada eritrosit, di antara 24 (80%) sampel E. coli menunjukkan α -hemolisin pada agar darah dan 2 (6,67%) sampel menunjukkan γ-hemolisis. Sebanyak 21 (70%) sampel E. coli mampu menghemaglutinasi eritrosit sapi dan 27 (90%) sampel mampu menghemaglutinasi eritrosit kelinci. Sebanyak 30 sampel E. coli tidak mampu menghemaglutinasi eritrosit ayam, kucing, anjing, dan manusia. Sebanyak 2 (6,67%) sampel menunjukkan hasil positif pada uji agregasi garam di antara 30 sampel E. coli dari M. fascicularis dan sisanya 28 (93,33%) sampel tidak menunjukkan hasil positif pada uji agregasi garam. Kesimpulan penelitian adalah Sebanyak 30 isolat E. coli menunjukkan variasi faktor virulensi yang berperan dalam mekanisme diare pada Macaca fascicularis. Terdapat 24 isolat (80%) yang menunjukkan α-hemolisin, 2 isolat (6,67%) menunjukkan γ-hemolisin, dan 4 isolat lainnya (13,33%) tidak menunjukkan aktivitas hemolisis. Selain itu, 29 isolat (96,7%) menunjukkan hemaglutinasi terhadap eritrosit sapi dan/atau kelinci, sedangkan 2 isolat (6,7%) bersifat hidrofobik berdasarkan uji salt aggregation.
PREVALENCE OF mcr-1 COLISTIN-RESISTANT GENE IN Escherichia coli ALONG THE BROILER MEAT SUPPLY CHAIN IN INDONESIA Palupi, Maria Fatima; Wibawan, I Wayan Teguh; Sudarnika, Etih; Maheshwari, Hera; Darusman, Huda Shalahudin
BIOTROPIA Vol. 26 No. 2 (2019): BIOTROPIA Vol. 26 No. 2 August 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2019.26.2.1054

Abstract

Colistin is the last drug of choice for dealing with carbapenem-resistant Enterobacteriaceae bacteria; hence, this drug is very crucial to human health. The discovery of a plasmid-mediated colistin-resistant gene, the mobilized colistin resistance-1 (mcr-1), signals a significant global health threat. Colistin sulfate is an antimicrobial agent which has been approved for use in broilers in Indonesia. Thus, this study aimed to measure the prevalence of colistin-resistant Escherichia coli and to detect the mcr-1 colistin-resistant gene in E. coli and E. coli O157:H7 in the entire supply chain of broilers in Bogor Regency, West Java Province, Indonesia. Samples were taken from 47 flocks that used colistin sulfate (47 pools of cloacal swabs, 47 pools of drinking water, and 47 pools of litter), seventy fresh meat samples and seven plucker swab samples from seven small-scale poultry slaughterhouses, seventy fresh meat samples from seven traditional markets, and seventy cooked meat samples from seven small restaurants. The isolation of E. coli was done on each of the 358 samples, and 493 isolates were obtained. All the E. coli isolates were then tested for their susceptibility to colistin sulfate by using the agar dilution method. The detection of the mcr-1 gene from the colistin-resistant isolates (minimum inhibitory concentration > 2 µg/mL) was conducted using the polymerase chain reaction (PCR). The prevalence value of colistin-resistant E. coli in all the isolates was 11.76% (CI 95%; CL 9.21–14.91), and the prevalence of the mcr-1 gene was 10.55% (CI 95%; CL 8.13–13.57%). A very good agreement correlation existed between the colistin-resistant phenotype and the mcr-1 gene (κ = 0.939). The mcr-1 gene was found in 89.66% of colistin-resistant E. coli isolates. Two colistin-resistant and mcr-1 carrying isolates were identified as E. coli O157:H7 serotype. This research was the first study attempt on the mcr-1 gene in Indonesia, covering the entire supply chain of broiler meat from farms to consumers. The results indicated the necessity to reduce the use of colistin sulfate in broiler management and to improve biosecurity measures, not only in farms but also in the entire supply chain of broiler meat production.
Hematological Profile of Macaca nemestrina Across Different Age Groups Sri Wahyuni; Fitriya Nur Annisa Dewi; Anita Esfandiari; Suryo Saputro; Huda Shalahudin Darusman
Acta VETERINARIA Indonesiana Vol. 13 No. 3 (2025): November 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.3.217-223

Abstract

Macaca nemestrina, also known as the pigtail macaque, plays a crucial role in drug development and serves as a highly valuable animal model for studying various human diseases due to its phenotypic similarities to humans, including those related to the aging process. Hematology is a critical component of clinical assessments for this spesies, serving both as a diagnostic tool to determine their health status and to characterize disease models, including degenerative and geriatric diseases. As anemia is a common phenomenon in the elderly, it is also important to determine whether M. nemestrina exhibit a similar condition with age. The aim of this study was to determine the hematological profiles of clinically healthy female M. nemestrina across young, adult, and elderly age groups, particularly related to anemia parameters. This study was conducted at the Primate Research Center, IPB University, Bogor, Indonesia. The animals were part of a breeding colony and were divided into three groups based on age: young (5–7 years), adult (12–14 years), and elderly (18–21 years). Following anesthesia with ketamine, a physical examination was performed to determine clinical status, and blood samples were collected for a complete blood count (CBC) evaluation using an automated analyzer. The results showed that total erythrocytes, hemoglobin concentration, hematocrit value, and mean corpuscular hemoglobin (MCH) were significantly different (P<0.05) in the elderly group compared to the other groups. Additionally, the red cell distribution width (RDW) percentage and leukocyte count differed significantly between the adult and elderly groups. The results showed that the erythrogram differs with age in clinically healthy M. nemestrina, suggesting the importance of determining appropriate baseline reference values in macaques of different ages.
Preclinical Evaluation of HPV Type 52 L1L2 Chimeric Protein as a Cervical Cancer Vaccine Candidate Sari, Isti Kartika; Pamungkas, Joko; Mustopa, Apon Zaenal; Wibawan, I Wayan Teguh; Mamangkey, Jendri; Chairunnisa, Sheila; Irawan, Herman; Hertati, Ai; Ekawati, Nurlaili; Umami, Rifqiyah Nur; Novianti, Ela; Nurfatwa, Maritsa; Darusman, Huda Shalahudin
HAYATI Journal of Biosciences Vol. 33 No. 3 (2026): May 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.3.556-565

Abstract

High-risk human papillomaviruses (HPVs) are the primary etiological agents of cervical cancer, accounting for more than 300,000 deaths annually worldwide. Current prophylactic vaccines based on recombinant L1 major capsid virus-like particles (VLPs) have demonstrated strong efficacy but are restricted to a limited spectrum of HPV types. To address this limitation, the present study evaluated a recombinant L1L2 chimeric protein derived from HPV type 52 as a potential candidate for a broad-spectrum vaccine. The chimeric protein was expressed in Escherichia coli strain BL21 (DE3) and purified for immunization studies. Female BALB/c mice (Mus musculus, n = 5 groups) were immunized, and immune responses were analyzed by enzyme-linked immunosorbent assay (ELISA) and pseudovirion-based neutralization assays (PBNA). The recombinant L1L2 vaccine candidate induced detectable antibody responses against HPV antigens; however, neutralizing activity remained modest. Histopathological analysis of liver and kidney tissues showed no evidence of toxicity, supporting the safety profile of the candidate. In summary, these results suggest that the HPV type 52 L1L2 chimeric protein represents a promising platform for the development of cervical cancer vaccines, although further optimization is required to achieve robust cross-neutralizing efficacy.
Anticancer Activity of Phanera semibifida Extract Evaluated by Brine Shrimp Lethality Test (BSLT) and Molecular Docking Studies Suhendra, Meyla; Juliandi, Berry; Darusman, Huda Shalahudin; Sadiah, Siti; Fitmawati, Fitmawati; Rianti, Puji
Journal of Health and Nutrition Research Vol. 5 No. 1 (2026)
Publisher : Media Publikasi Cendekia Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56303/jhnresearch.v5i1.850

Abstract

Indonesian people use plants as medicine because they are considered to have fewer side effects. The Lingga island community used Phanera semibifida (Roxb.) traditionally to make therapeutic drugs. P. semibifida has the potential to be a medicinal plant. We extracted stems and leaves with three different solvents and tested their cytotoxic activity. The ethanolic stem extract showed the highest value of LC₅₀ in the moderate toxicity category. The ethanolic steam extract continued to be subjected to in silico analysis, using molecular docking. We selected four compounds for molecular docking studies against five cancer-related proteins. The proteins are Estrogen receptor α, CDK2, Human Cytochrome P450 CYP17A1, Cyclin A, and BCL-2. The result showed that Ellagic acid bound strongly to Estrogen receptor α, CDK2, CYP17A1, and Cyclin A protein. Cyanidanol showed the highest affinity to BCL-2. These results suggest that P. semibifida contains compounds that can target multiple cancer-related proteins. Our findings highlight its potential as a source of anticancer agents. We recommend isolating these compounds and testing their efficacy through in vitro and in vivo studies to confirm their therapeutic potential in future studies.
Co-Authors A.S. Satyaningtijas Achmad, Yusuf Farid Adi Winarto Adi Winarto Agik Suprayogi Ahmad Kamal Nasution Ahmad Subhan Ai Hertati, Ai Akhiruddin Maddu Andhika Yudha Prawira Andi Hiroyuki Angela Mariana Lusiastuti Anita Esfandiari Annisa Dewi, Fitriya Nur Apon Zaenal Mustopa Aprilyana, Lia Ardiansyah Nurdin Benvika Berry Juliandi Chairunnisa, Sheila Damiana Rita Ekastuti Dayana, Hepy Dewi Ratih Agungpriyono Dewi, Fitriya Nur Annisa Dondin Sajuthi DYAH PERWITASARI -FARAJALLAH Ekawati, Nurlaili Ekowati Handharyani Elok Budi Retnani Entang Iskandar Entang Iskandar Entang Iskandar Entang Iskandar Ernes Andhesfa Etih Sudarnika Fadla, Ilma Nadya Fauzi Abdurrahman Munggaran Femke Den Haas Ferdinandez, Mariana Firdaus, Moh Egy Rahman Fitmawati Fitmawati Fitri, Arni Diana Habib Ilham Maulana Al Adhim Hana Intishar Sawitri Hardo Basuki Harrold Halilintar MR Subu Taopan Hasya, Karin katina HERA MAHESHWARI Hidayaturrahmah Hidayaturrahmah I wayan Teguh Wibawan Ice Lusia Marta IETJE WIENTARSIH Ikramullah, Muh. Chaeril Indra Bachtiar Indra Bachtiar Indriawati, Iin Iqbal Ngabdusani Irawan, Herman Irawan, Shasmita Irma Herawati Suparto Jendri Mamangkey Joko Pamungkas Jusak Nugraha Kanti Rahmi Fauziyah Katrin Roosita koekoeh santoso Krisnayanti, Ni Putu Eka Lina Elfita Lina Elfita Linda Tjhin Lis Rosmanah Loe, Fhady Risckhy Loes (ML) Schure Maria Fatima Palupi Maria Fatima Palupi Maria Fatima Palupi Megawati Iskandar Mia Audina Muhammad Hamdan Mustopa, Apon Z. Ningrum, Emilna Mega Novi Mayasari Noviana, Rachmitasari Novianti, Ela Novriyandi Hanif Nugroho, Setyo Widi Nurfatwa, Maritsa Perdhana, Ika Satya Permanawati Permanawati PERMANAWATI, PERMANAWATI Puji Rianti Putri, Indah Aprianti Rachmitasari Noviana Rahmania Hanim Rahminiwati, Min Randall C. Kyes Rasyida Ulfa Ratna, Lita Tri Ratni Ernita Ratu, Safira Pinaka Pramestika Raymond R. Tjandrawinata Ridzki M.F. Binol Rifqiyah Nur Umami, Rifqiyah Nur Rika Sudranto Rizal, Muhammad Farid Rizki Aulia Ansari Roostita L Balia Rosmanah, Lis Rosyid, Bahaudien Sadiah, Siti Sari, Isti Kartika Savitri Novelina Sela Septima Mariya Setyawaty, Dyah Setyo Widi Nugroho Sibit, Denniswara SILMI MARIYA Silmi Mariya Silmi Mariya Silvy Thiyana Soedarso, Yovi Alwi Sony Hartono Wijaya Sri Wahyuni Srihadi Agungpriyono Subu Taopan, Harrold Halilintar MR Suduri, Adam Suhendra, Meyla Sukenda Sukenda Sumitro - Sundari, Betty Suparto, Irma H. SURYA, MARYATI Suryo Saputro Tambunan, Anggitha Tatang Mitra Setia Tatik Mufidah Tommy Langgeng Abimanyu Tri Wibawa UUS SAEPULOH Uus Saepuloh Uus Saepuloh Vinka Aftinata Kusumaputri Wartika Rosa Farida Wasmen Manalu WIDANARNI WIDANARNI Wira, Dwi Wahyuda Yuliana Yuliana Yuliana Zaelani, Bella Fatima Dora