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Huda Shalahudin Darusman
IPB University
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Perilaku Sepasang Cephalopachus bancanus di Kandang Konservasi Pusat Studi Satwa Primata, Bogor Wira, Dwi Wahyuda; Fadla, Ilma Nadya; Hiroyuki, Andi; Mayasari, Novi; Iskandar, Entang; Darusman, Huda Salahudin; Farajallah, Dyah Perwitasari
Acta VETERINARIA Indonesiana Vol. 12 No. 1 (2024): Maret 2024
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.12.1.55-64

Abstract

Cephalopachus bancanus saat ini dikategorikan sebagai hewan rentan (vulnerable) oleh International Union for Conservation of Nature (IUCN). Penurunan populasi ini terjadi karena hilangnya habitat dan tingkat eksploitasi yang tinggi. Pemahaman perilaku Cephalopachus bancanus dalam konservasi dapat digunakan untuk menunjang perkembangbiakan satwa dalam kaitan mendukung upaya konservasi agar satwa endemik ini tidak menurun populasinya. Penelitian ini bertujuan untuk mengukur frekuensi perilaku sepasang Cephalopachus bancanus guna mendapatkan data dan informasi mengenai perilaku sepasang dalam kandang konservasi Pusat Studi Satwa Primata, Bogor. Penelitian perilaku pasangan yang teramati diantara lain: perilaku berkelompok, perilaku saling membersihkan diri, perilaku bertengkar, berselisih dan menghindar, perilaku bermain, perilaku seksual. Metode pengamatan yang digunakan adalah focal animal sampling dan instantaneous sampling dengan penilaian one-zerotime sampling. Hasil pengamatan selama 5 periode pengulangan waktu menunjukkan bahwa perilaku berkelompok mempunyai frekuensi paling tinggi yaitu 40% dengan durasi 134 menit, perilaku saling membersihkan diri menunjukkan frekuensi 37% dengan durasi 121 menit, perilaku bertengkar, berselisih dan menghindar menunjukkan frekuensi 18% dengan durasi 61 menit, perilaku bermain menunjukkan frekuensi 4% dengan durasi 12 menit, dan perilaku seksual menunjukkan hasil frekuensi paling rendah, yaitu 1% dengan durasi 4 menit. Kesimpulan dari penelitian ini yaitu perilaku sosial sepasang Cephalopachus bancanus paling banyak dilakukan yaitu perilaku berkelompok dan paling sedikit dilakukan yaitu perilaku seksual.
Myristicin Inhibit Invasion and Migration of Melanoma Cells through Suppression of MMP2 and MMP9 Gene Expression Krisnayanti, Ni Putu Eka; Mariya, Silmi; Indriawati, Iin; Dayana, Hepy; Zaelani, Bella Fatima Dora; Tjandrawinata, Raymond R.; Darusman, Huda Shalahudin
Science and Technology Indonesia Vol. 9 No. 4 (2024): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.4.884-892

Abstract

Melanoma is the deadliest type of skin cancer, having a high mortality rate. This cancer has an aggressive nature, is highly invasive, and has the tendency to metastasize. Matrix metalloproteinases (MMPs) are essential in that process, especially MMP2 and MMP9. Their expression is upregulated during metastasis progression. Myristicin is one example of a compound that can be utilized to target MMP 2 and MMP 9 in melanoma. This research concerns the activity of myristicin to inhibit melanoma cell invasion and migration by suppressing MMP2 and MMP9 gene expression. The MTT assay in this study demonstrated that myristicin exhibited strong cytotoxic activity against melanoma cells. This compound works in a dose-dependent manner by inhibiting cell migration and invasion. The invasion test was performed using the transwell assay, whereas the migration test was performed using the wound healing assay. The invasion assay results were consistent with MMP2 and MMP9 gene expression. These two genes were analyzed using the RT-qPCR technique. It has been demonstrated that low gene expression in melanoma cells inhibits cell invasion. In contrast, higher MMP2 and MMP9 gene expression was associated with an increase in the number of invasive cells on average. However, MMP2 and MMP9 in excessive expression and uncontrolled activity impair the ability of melanoma cells to form a monolayer sheet to cover wound gaps. This condition significantly reduced the migration rate and percentage of wound closure.
3D Culture Cells Technique for Exosomes Isolation of HEK293 and its Application on WiDr Cells Audina, Mia; Mariya, Silmi; Zaelani, Bella Fatima Dora; Yuliana; Darusman, Huda Shalahudin
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1173-1180

Abstract

Three-dimensional (3D) culture is a technique commonly utilized in bioprocessing and biomedical research. Exosomes have been investigated as carriers for medications in numerous studies employing 3D culture methodologies. The objective of this research is to employ 3D cell culture for the isolation and treatment of exosomes targeting colon cancer cells. The isolation of exosomes obtained from HEK293 cells was conducted through the ultracentrifugation technique. Subsequently, exosome treatment was administered to WiDr cells at concentrations of 3.5 µg/ml, 7 µg/ml, and 14 µg/ml.The validation of molecular markers of exosomes (CD9 and CD81), along with BAX, BCL-2, and CD133, was performed using qRT-PCR. The findings revealed the successful isolation of exosomes derived from HEK293 cells, which exhibited the expression of markers CD9 and CD81. Furthermore, the expression of BAX and BCL-2 indicated the potential of exosomes to induce apoptosis, while the expression level of CD133 decreased with treatment at varying concentrations. These results suggest that exosome treatment has the capability to impede the proliferation of WiDr cells and reduce the expression of CD133, thereby signifying the potential application of exosomes as an in-vitro model for investigating cancer therapy in the future.
Cloning, Expression, and Bioinformatics Modeling of Human Papillomavirus Type 52 L1/L2 Chimeric Protein in Escherichia coli BL21 (DE3) Ikramullah, Muh. Chaeril; Mustopa, Apon Zaenal; Wibawa, Tri; Hertati, Ai; Umami, Rifqiyah Nur; Ratna, Lita Tri; Irawan, Shasmita; Firdaus, Moh Egy Rahman; Darusman, Huda Salahudin
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.891-902

Abstract

Human papillomavirus (HPV) L1 major capsid protein generates a highly immunogenic virus like particles (VLPs), which have been used as the main component of its prophylactic vaccine. However, the neutralizing antibodies against L1 VLPs are mostly type specific and may not be effective to prevent infection from different strains of HPV. On the other hand, HPV L2 minor capsid protein has low antigenic variation, thus can induce cross-neutralization. This study aims to obtain HPV 52 L1/L2 chimeric protein, which is designed based on HPV type 52 as one of the most circulated high-risk types in Indonesia, to develop a broad-spectrum HPV vaccine. Substitution of HPV 52 H4 helix L1 region with an HPV 52 L2 epitope was carried out using overlap extension PCR. HPV 52 L1/L2 chimeric gene was constructed into pET-SUMO expression vector and expressed in Escherichia coli BL21 (DE3). Bioinformatics modeling suggested that L2 epitope was located inside of the loop region in monomer form, and on the contrary, it was located outside of the pentamer surface. Furthermore, B cell and T cell epitopes predictions were conducted using Immune Epitope Database (IEDB) analysis. The B cell epitopes prediction revealed eleven potential epitopes, whereas the T cell epitopes prediction showed seven potential epitopes for each MHC class I and MHC class II. This study showed that HPV 52 L1/L2 chimeric protein has the potential to induce cross-neutralizing antibodies and can be developed as a promising candidate for a new HPV vaccine.
RELIABILITY OF AMYLOID DETECTION IMMUNOASSAY KITS FOR ALZHEIMERS DISEASE SCREENING: A PRELIMINARY STUDY Putri, Indah Aprianti; Ningrum, Emilna Mega; Noviana, Rachmitasari; Mariya, Silmi; Saepuloh, Uus; Retnani, Elok Budi; Hamdan, Muhammad; Nugraha, Jusak; Darusman, Huda Shalahudin
Jurnal Kedokteran Hewan Vol 18, No 3 (2024): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i3.31109

Abstract

Alzheimers Disease is a progressive neurodegenerative disease that damages the brain and is part of dementia. Alzheimers dementia is caused by various factors that until now the proper therapy is uncertain. Kit production as a diagnostic tool to support the development of screening for Alzheimers disease is a strategic effort. Screening based on detection with Enzyme-Linked Immunosorbent Assay (ELISA) can work effectively and accurately to optimize a specific antibody material or monoclonal antibody to the A42 peptide. This study aimed to research the reliability of the amyloid detection kit which was developed as an alternative screening test for Alzheimers disease compared to commercially available kits. Based on the results and interpretation of inter and intra-assay coefficients, the in-house ELISA kit has comparable A42 detection to the commercially available ELISA kit
Edible Bird’s Nest Extract Reduced Expression of Senescence Markers in Bone Marrow Mesenchymal Stem Cells Elfita, Lina; Wientarsih, Ietje; Sajuthi, Dondin; Bachtiar, Indra; Darusman, Huda Shalahudin
JSFK (Jurnal Sains Farmasi & Klinis) Vol 8 No 1 (2021): J Sains Farm Klin 8(1), April 2021
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.8.1.19-26.2021

Abstract

Edible bird’s nest (EBN) is often consumed as a health food due to its suggested health benefits, including anti-aging effects, however the mechanism is still unknown. This study investigated the effect of EBN extract using long term expansion bone marrow-derived mesenchymal stem cells (BMMSCs) as an aging model. Passage 5 (P5) and passage 8 (P8) BMMSCs were treated with EBN extract, and their proliferation, senescence-associated β-galactosidase (SA-β-Gal) activity, and expression of p16INK4a were analyzed. Treatment of BMMSCs with EBN extract decreased population doubling time (PDT) in P5 but not in P8 BMMSCs. In P5 BMMSCs, 200 ppm EBN extract increased BMMSCs proliferation, with PDT reduced by 27.6%. However, 200 ppm EBN extracts did not affect P8 BMMSCs proliferation, although it increased BMMSCs viability. Treatment of P5 and P8 BMMSCs with 200 ppm EBN extract decreased SA-β-Gal activity by 54.8% and 47.1% of the control, respectively (P<0.05). Levels of p16INK4a expression were 5.4-fold lower in P5 BMMSCs treated with 200 ppm EBN extract compared to control (P<0.05). Similarly, treatment of P8 BMMSCs with 200 ppm EBN extract reduced p16INK4a mRNA level by 7.9-fold compared to the control (P<0.05). In order to investigate the pathway of EBN extract inhibition, we further analyzed IL-6 and NF-κB1 expression. Treatment of P5 and P8 BMMSCs with 200 ppm EBN extract reduced IL-6 mRNA levels by 7.9-fold and 2.1-fold of control, respectively (P<0.05). We found that 200 ppm EBN extract reduced NF-κB1 mRNA level approximately 2.4-fold both in P5 and P8 BMMSCs (P<0.05). Thus, EBN extract reduces markers of senescence, indicated by decreased SA-β-Gal activity and p16INK4a mRNA level, and this correlated with reduced messenger RNA levels of the pro-inflammatory factor IL-6 and the transcription factor NF-κB1.
Impact of Dietary Variation on Gut Microbiota Composition in Macaca fascicularis at the Primate Research Center, IPB University Tambunan, Anggitha; Rianti, Puji; Darusman, Huda Shalahudin; Kyes, Randall C.
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.670-682

Abstract

Long-tailed macaques (Macaca fascicularis: LTMs) are widely distributed, adaptable primates commonly used as animal models in biomedical research. At primate facilities such as the Dramaga Captive Breeding Facility and the Tinjil Island Natural Habitat Breeding Facility at the Primate Research Center, IPB University, LTMs experience varying diets depending on their captive or semi-wild environments. At Dramaga Captive Breeding Facility, LTMs housed in individual and group cages are provided with different feeding regimens, whereas the semi-wild LTMs on Tinjil Island feed primarily on natural food sources with occasional, very modest provisioning of bananas. Our study's objective is to evaluate these diets' influence on the gut microbiota and overall health of LTMs. Through comparative analysis of fecal microbiomes using 16S rRNA sequencing, we identified distinct gut microbiota profiles between captive and semi-wild LTMs. Semi-wild LTMs displayed a predominance of Proteobacteria, notably Succinivibrio, associated with a high-fiber diet derived from wild plant consumption. In contrast, captive LTMs housed in individual and group cages exhibited dominance of Firmicutes and Bacteroidota, respectively. These findings suggest that diet is pivotal in shaping gut bacterial profiles, potentially impacting metabolic functions. Our study provides insight into the relationship between certain diets and gut bacterial composition in LTMs across different breeding facilities at the Primate Research Center, IPB University, thereby offering valuable implications for LTMs' welfare and management strategies.
Analysis of Amino Acid Profiles in Bone, Oil, and Muscle Tissue and Wound Healing Activity of Pangasius hypophthalmus Oil in Rattus norvegicus Hidayaturrahmah, Hidayaturrahmah; Suprayogi, Agik; Hanif, Novriyandi; Roosita, Katrin; Darusman, Huda Shalahudin; Aprilyana, Lia
Borneo Journal of Pharmacy Vol. 8 No. 1 (2025): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v8i1.8182

Abstract

Patin (Pangasius hypophthalmus), a commercially important freshwater fish, is a rich source of protein containing both essential and non-essential amino acids, crucial for various biological functions. This study aimed to determine the amino acid composition of muscle tissue, oil, and bones of P. hypophthalmus and evaluate the wound healing potential of its oil extract in Rattus norvegicus. High-Performance Liquid Chromatography was employed to quantify the levels of essential and non-essential amino acids in each tissue. Results revealed that P. hypophthalmus oil exhibited the highest total amino acid content, particularly rich in histidine, arginine, and lysine. The wound healing activity of P. hypophthalmus oil extract was assessed in a rodent model, demonstrating a significant reduction in wound area (0.62-0.84 mm) and an impressive wound healing percentage (79-84.45%). These findings suggest that different parts of P. hypophthalmus possess valuable nutritional and therapeutic properties. Notably, the high amino acid content of P. hypophthalmus oil, particularly essential amino acids, highlights its potential as a promising source for developing pharmaceutical products, including amino acid supplements, wound healing agents, and formulations for metabolic support. Further research is warranted to fully explore the therapeutic potential of P. hypophthalmus oil and its bioactive components.
Prevalence of Gastrointestinal Endoparasites from Confiscated Long Tailed Macaque (Macaca fascicularis) Formerly Used as Dancing Monkey, Pet and Human Wildlife Conflict in Java Areas of Indonesia Ferdinandez, Mariana; Entang Iskandar; Huda Salahudin Darusman; Loes (ML) Schure; Habib Ilham Maulana Al Adhim; Benvika; Femke Den Haas
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 7, No 1, JANUARY 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v7i1.21753

Abstract

The purpose of this study is to identify the type, intensity, and prevalence of intestinal helminth infection in confiscated Long-tailed macaque (Macaca fascicularis). Thirty samples of feces from long-tailed monkeys (18 females and 12 males), with a total of 7 Dancing Monkeys, 5 Human Wildlife Conflict groups, and 18 Pet macaque groups. West Java's Cikole Animal Hospital collected samples aseptically, labeled them, and then analyzed them in its clinical laboratory. Using the floating method, samples were evaluated to determine the intensity of infection and the identification of parasites. The incidence of endoparasites in confiscated macaques is 20 %. The prevalence of endoparasites was highest in the individual group of pet macaques, at 27.78 %, and lowest in the Dancing Monkey group, at 14.29%. The prevalence of endoparasite infection was highest in the pet macaques, at 83.33 percent, compared to 16.67% among the dancing monkeys. In the human-infection conflict group, no endoparasite infections were detected. The prevalence of endoparasites in positive infected infant samples was as high as 75%, but the prevalence in sub-adult and adult age groups was 11% and 14%, respectively. From the number of positive samples, the male individual group had the highest percentage, 66.66 %, while the female individual group had the lowest proportion, 33.33 %. In general, the pattern of infection is the same, consisting of 50% double infection types and 50% single infection types. The average intensity of helminth infection in Strongyle spp. was 45.50±25.10 eggs per gram of feces, while in Trichuris spp., it was 120.00±0.00 eggs per gram of feces. Strongyle spp. infects 100% of the positive samples, while Trichuris spp. infects 50% of the samples.   Keywords: Macaca fascicularis, Confiscated macaque, Endoparasite, Prevalence  
Method Modification of Developing Ischemic Stroke Animal Models Using Intravenous Catheters in Rats Perdhana, Ika Satya; Handharyani, Ekowati; Darusman, Huda Shalahuddin; Nugroho, Setyo Widi; Maheshwari, Hera; Fitri, Arni Diana
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.67-76

Abstract

In various parts of the world, including Indonesia, the incidence of ischemic stroke remains high, and its impact poses a significant burden. This condition is influenced by numerous limitations in ischemic stroke therapy. Research on ischemic stroke is therefore crucial to develop, making the creation of appropriate animal models a vital necessity. One of the most commonly used methods by researchers to create animal models is the Middle Cerebral Artery Occlusion (MCAO) method. However, in Indonesia, its application remains limited due to the lack of available facilities. Method modifications can be implemented to enable the development of animal models in Indonesia. This study employed modifications to the MCAO method on eight Sprague Dawley rats. The modification involved using an intravenous catheter to facilitate the insertion of the filament as an occluder in the middle cerebral artery of the rats. Based on clinical and pathological observations, it can be concluded that the modified MCAO method used in this study is suitable for creating ischemic stroke animal models. With this modification, the creation of ischemic stroke animal models can be carried out in a simpler and more cost-effective manner.
Co-Authors A.S. Satyaningtijas Adi Winarto Adi Winarto Agik Suprayogi Ahmad Kamal Nasution Ahmad Subhan Ai Hertati, Ai Akhiruddin Maddu Andhika Yudha Prawira Andi Hiroyuki Angela Mariana Lusiastuti Apon Zaenal Mustopa Aprilyana, Lia Ardiansyah Nurdin Benvika Damiana Rita Ekastuti Dayana, Hepy Dewi Ratih Agungpriyono Dewi, Fitriya Nur Annisa Dondin Sajuthi DYAH PERWITASARI -FARAJALLAH Ekowati Handharyani Elok Budi Retnani Entang Iskandar Entang Iskandar Entang Iskandar Entang Iskandar Ernes Andhesfa Etih Sudarnika Fadla, Ilma Nadya Fauzi Abdurrahman Munggaran Femke Den Haas Ferdinandez, Mariana Firdaus, Moh Egy Rahman Fitri, Arni Diana Habib Ilham Maulana Al Adhim Hana Intishar Sawitri Hardo Basuki Harrold Halilintar MR Subu Taopan HERA MAHESHWARI Hidayaturrahmah Hidayaturrahmah I wayan Teguh Wibawan Ice Lusia Marta IETJE WIENTARSIH Ikramullah, Muh. Chaeril Indra Bachtiar Indra Bachtiar Indriawati, Iin Iqbal Ngabdusani Irawan, Shasmita Irma Herawati Suparto Jusak Nugraha Kanti Rahmi Fauziyah Karin katina Hasya Katrin Roosita koekoeh santoso Krisnayanti, Ni Putu Eka Lina Elfita Lina Elfita Linda Tjhin Lis Rosmanah Lis Rosmanah Loes (ML) Schure Maria Fatima Palupi Maria Fatima Palupi Maria Fatima Palupi Megawati Iskandar Mia Audina, Mia Muhammad Hamdan Ningrum, Emilna Mega Novi Mayasari Noviana, Rachmitasari Novriyandi Hanif Nugroho, Setyo Widi Pamungkas, Joko Perdhana, Ika Satya Permanawati Permanawati Puji Rianti Putri, Indah Aprianti Rachmitasari Noviana Rahmania Hanim Rahminiwati, Min Randall C. Kyes Rasyida Ulfa Ratna, Lita Tri Ratni Ernita Ratu, Safira Pinaka Pramestika Raymond R. Tjandrawinata Ridzki M.F. Binol Rifqiyah Nur Umami, Rifqiyah Nur Rika Sudranto Rizki Aulia Ansari Rosyid, Bahaudien Savitri Novelina Sela Septima Mariya Setyo Widi Nugroho SILMI MARIYA Silmi Mariya Silmi Mariya Silvy Thiyana Sony Hartono Wijaya Srihadi Agungpriyono Suduri, Adam Sukenda Sukenda Sumitro - Tambunan, Anggitha Tatang Mitra Setia Tatik Mufidah Tommy Langgeng Abimanyu Tri Wibawa UUS SAEPULOH Uus Saepuloh Uus Saepuloh Vinka Aftinata Kusumaputri Wartika Rosa Farida Wasmen Manalu WIDANARNI WIDANARNI Wira, Dwi Wahyuda Yuliana Yuliana Yuliana Zaelani, Bella Fatima Dora