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I Made Kardena
Faculty Of Veterinary Medicine Udayana University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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A Survey on Cases of Canine Transmissible Venereal Tumor (TVT) in Kintamani Dog Claudia Murti Andari; I Made Kardena; I Ketut Puja
Veterinary Science and Medicine Journal Vol 4 No 1 (2016)
Publisher : Udayana University

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Abstract

The cases of canine transmissible venereal tumor (TVT) in Kintamani Bali Dog were studied by evaluating the cases in clinical records from some veterinary clinics in the two regencies of Badung, Gianyar and Denpasar City. A total of 486 clinical records on Kintamani Bali Dogs gathered from 2010 to 2014 was used in this study. Among those, the specific cases on TVT diagnostic were observed. The study showed that the prevalence of TVT was catagorised low. Three of Kintamani Bali Dogs were diagnosed  to have TVT (0.62%). Canine transmissible venereal tumor affected both male and female dogs. The tumor cases were recorded in varied ages,  from 2 to 5 years. This result indicates that TVT in Kintamani Bali Dogs is likely to be associated with the dog’s sexual maturity.
Coconut Water Based Extender Effects on Motility,Viability, and DNA Integrity of Chilled Kintamani Dog Semen I Wayan Nico Fajar Gunawan; I Made Kardena; I Ketut Suatha; I Ketut Puja
Veterinary Science and Medicine Journal Vol 4 No 1 (2016)
Publisher : Udayana University

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Abstract

This research was conducted to investigate coconut water based extenders as an alternative extender for chilled Kintamani dog semen. Semen were collected from five dogs using manual stimulation and evaluated microscopically. Sperm were stored at 50C and divided into two aliquots which were extended with either coconut water based extender and sodium citrate extender in the ratio 1:2, 1:3 and 1:4. Sperm motility, viability and DNA integrity immediately and periodically (0,3 and 6 hour) were evaluated. Result showed that the effect of diluent on sperm motility and integrity of the Kintamani dog exerted a highly significant (p<0.01), whereas it had no effect on the percentage of dead sperm (p> 0.05). These results indicate that coconut water extender give good results in maintaining the motility, viability and DNA integrityof Kintamani dog sperm. It is concluded that coconut water suitable for extender of Kintamani dog.
Prevalensi Dan Gambaran Histopatologi Tumor Kulit Pada Anjing Di Kota Denpasar Edward Emanuel Mango; I Made Kardena; I Ketut Eli Supartika
Buletin Veteriner Udayana Vol. 8 No. 1 Pebruari 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Skin tumors grow uncontrollably, invades the skin or soft tissue under the skin, can be benign or malignant. This research was conducted by analyzing risk factors : age and type associated with histopathological changes of 113 samples of dogs with skin tumors in Denpasar in 2008-2012 are diagnosed from the Laboratory Pathologic of Balai Besar Veteriner in Denpasar Bali. From this study, it’s identified 15 types of skin tumors in dogs in Denpasar, with respective prevalence were lipoma 28.32%, histiocytoma 10.62%, squamous cell carcinoma 8.85%,lymphosarcoma 6.19%, melanocytoma 6.19%, sebaceous gland tumors 6.19%, fibroma 5.31%,fibrosarcoma 5.31%, hemangioma 4.42%, hemangiosarcoma 4.42%, trichoepthelioma 4.42%,perianal gland adenoma 3.54%, liposarcoma 2.65%, mastocytoma 2.65%, and papilloma 0.88%.Prevalence of skin tumor in dogs in Denpasar based on the breeds and age’s wasn’t significantly different, it means skin tumor can spread either local and non local breed dog’s and can infect allages in dog’s. The histopathological changes from each kinds of skin tumors was different depended from the type of cells that grew uncontrollably, however generally they had unregulated structure with differential cell in various levels in chromatin, nucleus, and cytoplasmic.
Pengaruh Pemberian Pegagan (Centella asiatica) Terhadap Gambaran Mikroskopis Ginjal Mencit yang Diinfeksi Salmonella typhi Komang Ariya Hendrayana; Ni Ketut Suwiti; I Made Kardena; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 5 No.1 Pebruari 2013
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Telah dilakukan penelitian mengenai pengaruh pemberian pegagan (Centella asiatica) terhadap gambaran mikroskopis ginjal mencit yang telah diinfeksi  Salmonella typhi. Penelitian menggunakan 24 ekor  mencit jantan berumur 8 – 12 minggu, dengan berat badan 20 – 35 gram, dibagi menjadi empat perlakuan dengan enam kali ulangan, yaitu kelompok P0 dengan pemberian aquades steril, kelompok P1 dengan pemberian pegagan 125 mg/kg bb, kelompok P2 dengan pemberian pegagan 250 mg/kg bb, dan kelompok P3 dengan pemberian 500 mg/kg bb. Setelah 14 hari seluruh mencit diinfeksi dengan S. typhi. Pada hari ke 15 dilakukan nekropsi untuk pengambilan sampel berupa ginjal dan dibuat preparat histologi. Selanjutnya dilakukan pengamatan terhadap gambaran mikroskopis yang meliputi perubahan : infiltrasi sel radang, perdarahan, degenerasi vakuola dan nekrosis. Metode pewarnaan menggunakan Haematoxylin dan Eosin (HE). Hasil penelitian menunjukkan perlakuan P0 berbeda nyata dengan P1, P2, dan P3. Demikian juga dengan derajat kerusakan ginjal tersebut. Semakin tinggi dosis pegagan yang diberikan semakin berpengaruh terhadap perbaikan struktur mikroskopis ginjal.
Kesembuhan Fraktur Tulang Femur Kelinci Pasca Implantasi Bahan Cangkok Demineralisasi Serbuk Tulang Sapi Bali Ni Putu Trisna Asih; I Wayan Wirata; Luh Made Sudimartini; Ida Bagus Oka Winaya; I Made Kardena; I Wayan Gorda
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (663.053 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p13

Abstract

The purpose of this study to determine the microscopic changes in rabbit femur fracture healing post-implantation of the graft material demineralized powder bone of bali cattle. Twelve local male rabbits were divided into two groups randomly. Group A consisted of three rabbits used as a control, in which the diaphysis rabbit femur bones were drilled with a diameter of 5mm and depth of drill to reach the medulla, without giving graft material. Group B nine rabbits that were drilled the same as group A and given a powder bone graft. Monitoring progress of bone healing was done in a row against one rabbits in group A and group B three rabbits at week 2nd, 4th, and 6th post-surgery with biopsy for sampling bone subsequently prepared to see microscopic changes the bone with using hematoxylin-eosin (HE) staining. The results showed that the group B bone healing observ by reducing inflammation, proliferation of fibrolast, osteoblasts, osteoclasts, osteocytes, woven bone, trabecular bone, and neovascularization. In conclusion, the origin of the bone graft material bali cattle was able to induce the healing process of bone fractures in rabbits.
Pengaruh Konsumsi Urin Sapi Bali Terhadap Kadar Blood Urea Nitrogen, Kreatinin Serta Gambaran Histopatologi Ginjal Tikus Desi Elrini Sarah Alunat; I Made Kardena; I Nyoman Suarsana
Buletin Veteriner Udayana Vol. 6 No. 2 Agustus 2014
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

This study was conducted to determine the effect of bali cattle urine consumption to blood urea nitrogen and creatinine levels and  histopathological observation on rat kidney. A total of 20 male white rats were used in this study. They were divided into 4 groups : K1 was given distilled water and feeded libitium, while K2,K3,K4 were given bali cattle urine at dose 0,5; 1; and 2 cc/head/day for 30 days respectively. At the end of the treatment period, the all rats were euthanized with cetamine and Blood was collected to obtain plasma. Plasma was analyzed on the content of blood urea nitrogen, and creatinine. Kidneys was taken for histopathological ??with hematoxylin-eosin staining. The results showed that administration of bali cattle urine of a dose 2cc/head/day can lead to increased levels of blood urea nitrogen and creatinine. On the observation of kidne histopathology showed hemorrhage intertubular and fatty degeneration.
Prevalensi Dermatitis Ulseratif pada Tukik Lekang yang Dipelihara di Turtle Conservation and Education Centre Serangan Annabella Ruth Wijaya; Ida Bagus Windia Adnyana; I Made Kardena
Buletin Veteriner Udayana Vol. 10 No. 1 Pebruari 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (776.953 KB) | DOI: 10.24843/bulvet.2018.v10.i01.p09

Abstract

Ulcerative dermatitis is a disease that most commonly affects turtles and/or hatchlings that are kept in ponds, including in Turtle Conservation and Education Center (TCEC), Serangan. A study was conducted to determine the prevalence, pathology, and lesion location of ulcerative dermatitis in olive ridley hatchlings reared in TCEC Serangan, also the differences of the body condition index of olive ridley hatchlings with ulcerative dermatitis and healthy ones. Prevalence was determined by counting sick hatchlings compared with the total number of hatchlings at TCEC. Morphometry (straight carapace length, straight carapace width, curved carapace length, curved carapace width) and body weights were measured to determine the body condition index, and then body index differences were compared with unpaired T-Test. Skin tissue samples were processed into pathology slides and routinely staining of hematoxylin eosin (HE). The prevalence of ulcerative dermatitis in olive ridley hatchlings at TCEC was 16.2%. Crusty-yellow wound lesions of 2 mm to 2 cm in diameter were found and microscopically there were heterophils and mononuclear cells infiltration in skin dermis accompanied by erosion, parakeratosis, and necrotic material containing cell debris. Lesions were found mostly on neck area (63.04%), followed by flippers, head, skin near carapace, eyelids, and neck area with flippers combined. There was a very significant difference in body condition index between suffered hatchlings with healthy ones, where the average body condition index of suffered hatchlings were greater than healthy ones. Rearing management should be fixed.
Efek Toksisitas Ekstrak Daun Sirih Merah Terhadap Gambaran Mikroskopis Ginjal Tikus Putih Diabetik yang Diinduksi Aloksan Ni Made Rina Yulinta; Ketut Tono Pasek Gelgel; I Made Kardena
Buletin Veteriner Udayana Vol. 5 No. 2 Agustus 2013
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Penelitian ini bertujuan untuk mengetahui efek toksik dari ekstrak daun sirih merah terhadap gambaran mikroskopis ginjal tikus putih diabetes mellitus yang diinduksi aloksan. Sebanyak 20 ekor tikus putih jantan galur Sprague-dawley umur ± 3 bulan digunakan dalam penelitian ini. Seluruh sampel tersebut dibagi secara acak menjadi lima kelompok perlakuan yaitu: (P0) tikus sehat yang hanya diberikan aquades; (P1) tikus yang diberikan aloksan 120 mg/kg bb/intraperitoneal; (P2) tikus yang diberikan aloksan 120 mg/kg bb/intraperitoneal dan ekstrak daun sirih merah 50 mg/kg bb/peroral; (P3) tikus yang diberikan aloksan 120 mg/kg bb/intraperitoneal dan ekstrak daun sirih merah 100mg/kg bb/peroral; (P4) tikus yang diberikan aloksan 120mg/kg bb/intraperitoneal dan suspensi glibenklamid 1 mg/kgbb/peroral. Perlakuan diberikan selama 30 hari. Pada hari ke-31 semua tikus dieuthanasi dan dinekropsi untuk melihat gambaran mikroskopis ginjal. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun sirih merah (Piper crocatum) dosis 50 mg/kg bb dan 100 mg/kg bb tidak menunjukkan perubahan patologi terhadap gambaran mikroskopis ginjal. Hal ini menunjukkan ekstrak daun sirih merah dosis 50 mg/kgbb dan dosis 100 mg/kgbb tidak toksik terhadap jaringan ginjal tikus putih diabetes mellitus.
Studi Histopatologis Lambung Tikus Putih yang diberi Parasetamol dan Suplementasi Propolis Noviriolla Maria; I Ketut Berata; I Made Kardena; Samsuri Samsuri
Buletin Veteriner Udayana Vol. 9 No. 1 Pebruari 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Propolis is a substance produced by bees that has some benefits, such as: antioxidants and antiulkus. Paracetamol is a relatively safe drug for gastric, but in some studies claimed that high doses of paracetamol increase the risk of gastric mucosal disorders, such as: ulceration, desquamation, and erosion. The purpose of this study is to determine the effects of propolis in histopathological gastric rats that were given high doses of paracetamol. This study used a completely randomized design. A sample of 25 male rats were divided into 5 groups i.e.: a negative control without treatment paracetamol (P0), a positive control (P) that was given paracetamol 250 mg/kgbw, and three other groups given treatment with paracetamol 250 mg/kgbw with a variation of propolis, P1 (0.05 ml/rat), P2 (0.10 ml/rat), and P3 (0.15 ml/rat). The treatment was given in 10 days, then necropsy was done and gastric organs were taken. Histologic gastric specimen for histopathological examination was made by using the Kiernan method (1990) and stained with hematoxylin eosin (HE). Variables examined include histopathological lesions of erosion, desquamation, and ulceration of the gastric epithelium. Results indicate that in treatment P severe damage to the gastric (desquamation) has occurred, while the entire treatment P1, P2, and P3 show the repair from the damage caused by the paracetamol. Treatment P3 gives the best results in reducing the effects of the paracetamol. This study concluded that the provision of propolis may reduce the histopathological side effects on white rat’s gastric givenhigh dose of paracetamol.
GAMBARAN PATOLOGI PARU-PARU PADA ANJING LOKAL BALI YANG TERINFEKSI PENYAKIT DISTEMPER I Made Kardena; IB Oka Winaya; I Ketut Berata
Buletin Veteriner Udayana Vol. 3 No. 1 Pebruari 2011
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Canine distemper is a contagious disease, which can infect any dog species includingdomestic dog in Bali. This research used ten domestic dogs in Bali, which were naturallyinfected with canine distemper virus and have been positively confirmed by laboratory testusing RT-PCR method. Lung tissue changes have been identified on gross pathologicalobservation, i.e.: on average the lungs were changed in color and size. The lungs mainlychanged into pale or darker; in addition, the lungs tended to become bigger. Mean while,the septa alveoli of the lungs observed thicker and were infiltrated by inflammatory cells.This tissue changes indicated that canine distemper virus which infected domestic dog inBali caused inflammatory reaction in lung, which is known as interstitial pneumoniainterstitial. These changes implied to be related to the disturbance of the respiration tract,which was accompanied by mucus-purulent exudates
Co-Authors Abdul Azis Nasution Abdul Azis Nasution Abriansyah, Mohammad Ghaiz Adiluhur, I.M.P. Agastia, I.P.A. Agastia, P. A. A. Aida Lousie Tenden Rompis Amelia Avianti Saritjang Anak Agung Ayu Mirah Adi Anak Agung Gde Oka Darmayudha Anak Agung Gde Oka Darmayudha Anak Agung Gde Oka Dharmayudha Anak Agung Gede Sugianthara Anggreni, Ni Kadek Wiwik Annabella Ruth Wijaya Annas Farhani Arhiono, Haru Nira Putra Arini Nurhandayani Bambang Pontjo Priosoeryanto Bayu Setiabudi Berata , I Ketut Boedi P. Yenniastuti, Boedi P. Calvin Iffandi Calvin Iffandi Chanda Preanger, Chanda Claudia Murti Andari Desak Nyoman Dewi Indira Laksmi Desi Elrini Sarah Alunat Deska Gilang Mustika Dewa Ayu Swastini Dewa Ayu Widia Kusuma Ningrat, Dewa Ayu Widia Kusuma Dewi, I. A. M. Dewi, I.A.M.K. DWI SURYANTO Edward Emanuel Mango Eldarya Envisari Depari Elyda . Endris Arif Wicaksono, Endris Arif Farhan Abdul Hasan, Farhan Abdul Fathurohman Nasrudin Ferbian M. Siswanto, Ferbian M. Ferbian Milas Siswanto Findri Andriani, Findri Fitri Irawan Rahmawandani Gunawan, I Wayan Nico Fajar Gusti Ayu Yunianti Kencana Gusti Ayu Yuniati Kencana Hendrina Konda M Meha, Hendrina Konda M Herbert . Herbert . Hidayatullah, Lalu Syarif I Dewa Gde Tara Damayanthi, I Dewa Gde Tara I Dewa Made Adhiwitana I Gede Hendra Prasetya Wicaksana I Gusti Agung Arta Putra I Gusti Agung Ayu Suartini I Gusti Agung Gede Putra Pemayun I Gusti Ketut Suarjana I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Ketur Berata I Ketut Berata I Ketut Eli Supartika I Ketut Gunata I Ketut Puja I Ketut Suatha I Made Damriyasa I Made Dodi Gunawan I Made Dwinata I Made Merdana I Made Sukada I N. T. ARIANA I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Putu Cahyadi Putra, I Putu Cahyadi I Putu Gede Yudhi Arjentinia I Putu Wiralaga I Wayan Batan I Wayan Gorda I Wayan Suardana I Wayan Sudiarta I Wayan Sudira I Wayan Wirata I. H. U Utama I.K. Berata Ida Bagus Kade Suardana Ida Bagus Made Oka Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Imam Sobari imam sobari Indahyani, Ni Made Dwi Indrawan, Hieronimus Irhas, Rajiman Iwan Harjono Utama Janardani, Ni Made Kunti Jayawardhita, Anak Agung Gde Jihadulhaq, Jihadulhaq Kadek Karang Agustina Kartika, Erena Hajar Ketut Budiasa Ketut ELI Supartika Ketut Santhia Adhy Putra Ketut Tono Pasek Gelgel Komang Andika Purnama Komang Ariya Hendrayana Kristi Agusti Putri, Kristi Agusti Lagho, Engelbertus Efraim Luh Gde Sri Surya Heryani Luh Made Sudimartini Mahadewi, S. A. Mahadewi, S.A. Mahyuzar Mahyuzar Muhamad Furkam Fadilah Nallakrishna, I P. A. Ni Ketut Suwiti Ni Luh Eka Setiasih Ni Luh Watiniasih Ni Made Rina Yulinta Ni Nyoman Werdi Susari Ni Putu Ariantari Ni Putu Trisna Asih Ni Wayan Apsari Shantika Pratistha Ni Wayan Listyawati Palgunadi Ni Wayan Listyawati Palgunadi Novianti, Arindita Niatazya Noviriolla Maria Nugraha, Elisabeth Yulia Nur Hanifah Septiani, Nur Hanifah Nurrohman, Fahmi Galuh Nurul Faiziah Nurul Faiziah P. A. C. Kusuma P. A. C. Kusuma, P. A. C. P. Sampurna Paranitha, Dewa Ayu Patrisius Yanuaris Lada Salasa, Patrisius Yanuaris Prabandari, Anak Agung Istri Vera Pranatha, Wahid Danang PRANSIKA EKSY YONITA Purnama, Komang Andika Purnata, I Dewa Nyoman Alit Purwani, S. T. D Purwani, S. T. D. Putri Udayani Putu Agus Trisna Kusuma Antara Putu Dede Yudi Utama, Putu Dede Yudi Putu Mira Puspitayani Rasdiyanah . Rasdiyanah . Ratih, Dwi Kusuma Komala Rui Daniel de Carvalho Rusmin Indra S. T. D Purwani, Sakura, Yoakim Wilson Samiantara, I. K. Samsuri Samsuri Samuel Leonardo Silitonga Sari, Ni Komang Wahyu Centika Septianingsih, Rayni Silaban, Jesiaman Sri Kayati Widyastuti Sri Milfa Sri Milfa STEVEN YOHANES BOGIA TJOKORDA ISTRI AGUNG Cintya Dalem Tri A. Putra, Tri A. Tri Adi Putra, Tri Adi Wahyu Semadi Putra Widiarta, Lutfi Widyasanti, Ni Wayan Helpina Yanti, I G. A. A. D. Yasunobu Matsumoto Yesi Veronica Sitepu Yoga Eka Prasetyo Yoshihiro Hayashi Yuliantari, Ida Ayu Made Yunita Lestyorini Yunita Lestyorini