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I Made Kardena
Faculty Of Veterinary Medicine Udayana University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Efektivitas Plester Luka Pada Aplikasi Penutup Luka Insisi Pasca Operasi (EFFECTIVENESS OF WOUND PLASTER FOR COVERING POST-SURGERY INCISION WOUND) Deska Gilang Mustika; I Made Kardena; I Gusti Agung Gde Putra Pemayun
Buletin Veteriner Udayana Vol. 7 No. 2 Agustus 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

A study on the difference of wound incision healing in mice (Ratus novergicus) between the wound covered with a plaster compared to the wound that was closed using simple interruptedsutures in terms of macroscopic and microscopic observation has been done. A total of 16 malewhite rats with a weight range of 200 gram randomly divided into two groups. Incision was madeinto linea alba to both group. Incision at linea alba was closed with simple interupted suture, and subcutan was closed with subcutaneous suture using vicril 4/0. The first group of the skin incisionclosed with wound plaster, while the second group the incision is closed with simple interruptedsutures using non-absorbable thread. Results showed that no difference was observed between thewound that covered with plaster compared to the wound that closed with simple interruptedsutures. However, there was a significant difference on the density of collagen in microscopicexamination.
Kadar Logam Berat Pb dan Histopatologi Limpa Sapi Bali yang Dipelihara di Tempat Pembuangan Akhir Suwung Denpasar Wahyu Semadi Putra; I Ketut Berata; I Made Kardena
Buletin Veteriner Udayana Vol. 10 No. 1 Pebruari 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1215.95 KB) | DOI: 10.24843/bulvet.2018.v10.i01.p10

Abstract

Bali cattle eats organic and inorganic waste in Suwung Denpasar landfill as source of feed. The waste was suspected contaminated by heavy metals Pb. The aim of this study is to determine levels of heavy metals Pb and spleen histopathologic changes of bali cattle reared in Suwung Denpasar landfill. Spleen samples were collected from 5 cattle for examination of heavy metals Pb by Atomic Absorption Spectrofotometri method and histopathology preparations made by Hematoxilin-Eosin staining. The results showed the presence of heavy metal Pb contamination which varied of 1.5024 ppm, 1.5002 ppm, 2.0267 ppm, 2.0268 ppm and 0.6818 ppm. Microscopically, showed histopathological changes the form of depletion and proliferation of lymphoid cells.
Pengaruh Pemberian Ekstrak Etanol Daun Ashitaba Terhadap Histopatologi Lambung Mencit Jantan (THE EFFECT OF ETHANOL EXTRACT OF ASHITABA LEAVES TOWARD THE GASTRIC HISTOPATHOLOGY OF MALE MICE) I Putu Wiralaga; I Wayan Sudira; I Made Kardena; Anak Agung Gde Oka Darmayudha
Buletin Veteriner Udayana Vol. 7 No. 1 Pebruari 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

This study was intended to find out the effect of ethanol extract of Ashitaba leaves (Angelicakeiskei) toward the gastric histopathology of male mice. After being adapted for one week, 25 malemice (Mus musculus) were divided randomly to be five treatments. The first treatment was not given ethanol extract of Ashitaba leaves as control. The second till fourth treatment was given eachdose of 125; 250; 500; dan 1,000 mg/kg bb during 21 days orally. Necropsy for gastric organharvesting was conducted on the day of 22. The result of giving ethanol extract of Ashitaba leaves(Angelica keiskei) with a dose of 125 mg/kg bb showed that three mice undergo degeneration andinfiltration of inflammatory cells and one mice undergo necrosis. The dosing of 250 mg/kg bbshowed that three mice undergo degeneration and two mice undergo infiltration of inflammatorycells and necrosis as well. The dosing of 500 mg/kg bb showed that three mice undergodegeneration, infiltration of inflammatory cells, and necrosis. The dosing of 1,000 mg/kg bbshowed that three mice undergo degeneration and necrosis, and four mice undergo infiltration ofinflammatory cells. Based on the results of the study, It was concluded that the ethanol extract ofleaves Ashitaba dose of 125 mg / kg bw up to 1,000 mg / kg bw for 21 days did not cause structural disorders of the stomach organ histopathology of mice (Mus musculus) male. 
THE ADVANTAGES AND DISADVANTAGES OF SYNDROMIC SURVEILLANCE METHODS I Made Sukada; I Made Kardena
Buletin Veteriner Udayana Vol. 3 No.2 Agustus 2011
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Syndromic surveillance is surveillance methods which has the potential to detect diseasesoutbreak in the early stages. The systems used existing data of disease syndromes whichgenerally appear at the beginning of infection to provide immediate analysis. Additionally,the method may identify the disease outbreak earlier than the conventional surveillancewhich commonly requires a longer time to determine the cause of the outbreaks. Althoughmany experts believe that syndromic surveillance is a good method for early detection ofdisease outbreak, some of them also believe that this system has several drawbacks
Gambaran Histopatologi Hepar Mencit Yang Diberikan Ekstrak Etanol Sarang Semut Yoga Eka Prasetyo; I Made Merdana; I Made Kardena; I Wayan Sudira
Buletin Veteriner Udayana Vol. 11 No. 1 Pebruari 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.817 KB) | DOI: 10.24843/bulvet.2019.v11.i01.p08

Abstract

Anthill plants attracted many people as an alternative medicine because proven efficacious for treating tumor diseases, cancer, tuberculosis, stroke, coronary heart disease, diabetes mellitus, nosedleeds, ulcer, hemorrhoids, facilitate breastfeeding, increase stamina and sexual arousal. This study aimed to determine the effect of ethanol extract of the anthill plant to the mice liver histopathology. This study is an experimental laboratory. Twenty fourth male mice 10-12 weeks of age with a body weight of 25-35 grams were divided into 4 groups, each group consisting of 6 mice were given treatment that Control Group, Group I (doses 100mg/kg BW), Group II (doses 200mg/kg BW) and Group III (doses 300mg/kg BW) for 21 days. Hepatic histopathological changes were observed and assessed by histological damage in the form of infiltration of inflammatory cells, lipid degeneration and necrosis. Data were analyzed using statistical test of Kruskal-Wallis followed with Mann-Whitney Test (p<0.05). Kruskal-Wallis test result showed ethanol extract anthill very significant (p<0.01) effect on the incident of lipid degeneration, necrosis and infiltration of inflammatory cell of liver cells. Based on these result it can be concluded that the ethanol extract of anthill can cause histological changes in the liver of mice.
PENINGKATAN PENDAPATAN PETERNAK MELALUI PERBAIKAN MANAJEMEN PETERNAKAN BABI Kadek Karang Agustina; I Wayan Wirata; Anak Agung Gde Oka Dharmayudha; I Made Kardena; Nyoman Sadra Dharmawan
Buletin Veteriner Udayana Vol. 8 No. 2 Agustus 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

This research aims were to increase house hold income of pig farmer by reconstructing of the pig management systems. The methods used in this study were the application of the problem solving techniques through IPTEKDALIPI programs. The captured data were all expenses incurred and income received in one pig production period before and after intervention. The results showed that there was a significant increase in the income of the farmer from 31.4% to 38.77%. It could be concluded that the pig farmer’s income can be increased through improved management of pig farming systems.
SYNDROMIC SURVEILLANCE: A POTENTIAL METHOD FOR ALTERNATIVE ANIMAL HEALTH SURVEILLANCE SYSTEM I Made Kardena
Buletin Veteriner Udayana Vol 2 No. 1 Pebruari 2010
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Syndromic surveillance is an alternative surveillance system which can potentially detectanimal disease outbreaks in the early stages. Although syndromic surveillance haslimitations for the detection of sub-clinical and specific diseases, the method has the abilityto detect disease outbreaks rapidly including clinical emerging issues. In addition,syndromic surveillance is a suitable surveillance tool that can be applied in developingcountries since it is a low-cost surveillance method and is an option for poorly-resourcedveterinary services.
HISTOPATOLOGI HEPAR TIKUS PUTIH (Rattus novergicus) SETELAH PEMBERIAN EKSTRAK SARANG SEMUT (Myrmecodia pendans)YANG DIINDUKSI PARACETAMOL DOSIS TOKSIK I Made Merdana; I Made Kardena; Ketut Budiasa; I Made Dodi Gunawan
Buletin Veteriner Udayana Vol. 11 No. 1 Pebruari 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.244 KB) | DOI: 10.24843/bulvet.2019.v11.i01.p03

Abstract

This study aim was to determine the influence ant nest plant extract (Myrmecodia pendans) on histopathological changeof white rat liver (Rattus novergicus) due to induced with paracetamol toxic dose. This study used 24 male white rats, divided into four groups, negative control group (P0) given placebo, positive control group (P1) given paracetamol dose 250 mg / kg bw for 10 days, P2 given ant nest extract 250 mg / kg bw and paracetamol dose 250 mg / kg bw for 10 days, P3 given ants nest extract 250 mg / kg bw for seven days, then continued by giving paracetamol and ants nest extract with dose 250 mg / kg bw for ten days. After the treatment done, all the rats were dinecropsed. Liver organs were taken and processed for making histopathology preparations. Parameters examined included hemorrhage, congestion, degeneration and necrosis. The data obtained were analyzed statistically by using Kruskal Wallis test followed by Mann Whitney test. Mann Whitney test results for all categories of histopathologic changes in hemorrhagic, congestion, degeneration, and necrosis between negative control group (P0) and positive control group (P1) were significantly different (P <0.05), between negative control (P0) with P2 and P3 there was no significant difference (P> 0,05). Afterward, between the positive control (P1) and P2 with P3 there was a significant difference (P <0.05). I can be concludedthat the administration of paracetamol dose 250 mg/kg bw for 10 days affects the histopathologic changes of white rat liver. The administration of ant nest plant extracts can reduce the side effects of toxic doses of paracetamol.
Studi Histopatologi Limpa Anjing Penderita Distemper Dikaitkan Dengan Sebaran Sel-Sel Radang Pada Otak Dan Paru (HISTOPHATOLOGICAL STUDY OF SPLEEN ON DOGS INFECTED WITH DISTEMPER ASSOCIATED TO INFLAMATION IN THE BRAIN AND LUNGS) Muhamad Furkam Fadilah; I Ketut Berata; I Made Kardena
Buletin Veteriner Udayana Vol. 7 No. 2 Agustus 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

This study aim was to determine the distribution of inflammatory cells in canine distemper in terms of the level of inflammation in the spleen, brain and lungs. The sample used 20 infected dogs wihich from the spleens, brains, and lungs of the dogs were collected. These organs were processed for histophatological observation using harris hematoxilyn-eosyn stain. The inflammation of the organs examined by using binocular microscope with 200X magnification. the results showed that inflammation was observed in the spleens: 9 samples (45%) showed the presence of lymphoidcells that experienced a mild inflammation 7 (35%) moderate inflammation, and 4 (20%) severe inflammation in brain, 3 samples (15%) did not show observe inflammation, 12 (60%) mild, 5(25%) moderate inflammation, and not observed any severe inflammation in brain, pulmonary: 6(30%) mild inflammation, 11 (55%) moderate inflammation, and 3 (15%) severe inflammation. It can be concluded that the inflammation was observed microscopically in the spleen, brain and lungin the dog that infected with canine distemper virus
Kajian Toksisitas Sub Akut Ekstrak Etanol Anggur Bali pada Tikus Wistar Luh Made Sudimartini; I Wayan Nico Fajar Gunawan; I Wayan Wirata; I Made Kardena; Anak Agung Gde Oka Darmayudha; Amelia Avianti Saritjang
Buletin Veteriner Udayana Vol. 13 No. 1 Pebruari 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.498 KB) | DOI: 10.24843/bulvet.2021.v13.i01.p02

Abstract

Bali grapes are a source of antioxidants with high flavonoids and anthocyanins, which have many health benefits such as lowering cholesterol and anti-cancer, inhibiting aging, and protecting the heart. Many people consume Balinese grapes by eating directly, drink in the form of juice or extract form because of its benefits. The purpose of this study was to determine the sub-acute toxicity of the ethanol extract of bali grapes (Vitis vinera cv. Alphonso lavelle) to changes in SGOT and SGPT of male Wistar rat liver. This test includes a sub-acute toxicity test and a pharmacological effect test. This research is an experimental study with a completely randomized design using a post-test-only control group design. The number of white rats used in this study were 24 rats, which were divided into four groups of control groups, groups of mice given ethanol extract of bali grapes 0.3 g / ml/kg BW (P1), groups of mice given ethanol extract of grapes bali 3 g / ml/kg BW (P2), and the group of rats that were given 30 g / ml kg BW (P3) of ethanol extract of bali grapes. Each group consisted of 6 white male rats. In this study, the grape ethanol extract was presented orally once per day for 28 days. After 28 days of treatment, Wistar rats were euthanized, and blood samples were taken through Chantus Medial Orbitalis and then put into non-EDTA tubes. The blood sample that has been centrifuged and becomes serum is then inserted into the Eppendorf tube, which is used to test the levels of SGOT and SGPT. The results of the One-Way ANOVA analysis on SGPT levels in the liver of rats showed a significant difference (P <0.05), so that further analysis was necessary using the Duncan-test. Based on the results of the analysis using the Duncan Test, changes occurred in the P3 treatment group of rats given the ethanol extract of Bali grapes at a dose of 30 g / ml/kg BW, which was significantly different from the control treatment group (P0) so that the mixture of these materials was included in the class of materials that were practically non-toxic.
Co-Authors Abdul Azis Nasution Abdul Azis Nasution Abriansyah, Mohammad Ghaiz Adiluhur, I.M.P. Agastia, I.P.A. Agastia, P. A. A. Aida Lousie Tenden Rompis Amelia Avianti Saritjang Anak Agung Ayu Mirah Adi Anak Agung Gde Oka Darmayudha Anak Agung Gde Oka Darmayudha Anak Agung Gde Oka Dharmayudha Anak Agung Gede Sugianthara Anggreni, Ni Kadek Wiwik Annabella Ruth Wijaya Annas Farhani Arhiono, Haru Nira Putra Arini Nurhandayani Bambang Pontjo Priosoeryanto Bayu Setiabudi Berata , I Ketut Boedi P. Yenniastuti, Boedi P. Calvin Iffandi Calvin Iffandi Chanda Preanger, Chanda Claudia Murti Andari Desak Nyoman Dewi Indira Laksmi Desi Elrini Sarah Alunat Deska Gilang Mustika Dewa Ayu Swastini Dewa Ayu Widia Kusuma Ningrat, Dewa Ayu Widia Kusuma Dewi, I. A. M. Dewi, I.A.M.K. DWI SURYANTO Edward Emanuel Mango Eldarya Envisari Depari Elyda . Endris Arif Wicaksono, Endris Arif Farhan Abdul Hasan, Farhan Abdul Fathurohman Nasrudin Ferbian M. Siswanto, Ferbian M. Ferbian Milas Siswanto Findri Andriani, Findri Fitri Irawan Rahmawandani Gunawan, I Wayan Nico Fajar Gusti Ayu Yunianti Kencana Gusti Ayu Yuniati Kencana Hendrina Konda M Meha, Hendrina Konda M Herbert . Herbert . Hidayatullah, Lalu Syarif I Dewa Gde Tara Damayanthi, I Dewa Gde Tara I Dewa Made Adhiwitana I Gede Hendra Prasetya Wicaksana I Gusti Agung Arta Putra I Gusti Agung Ayu Suartini I Gusti Agung Gede Putra Pemayun I Gusti Ketut Suarjana I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Ketur Berata I Ketut Berata I Ketut Eli Supartika I Ketut Gunata I Ketut Puja I Ketut Suatha I Made Damriyasa I Made Dodi Gunawan I Made Dwinata I Made Merdana I Made Sukada I N. T. ARIANA I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Putu Cahyadi Putra, I Putu Cahyadi I Putu Gede Yudhi Arjentinia I Putu Wiralaga I Wayan Batan I Wayan Gorda I Wayan Suardana I Wayan Sudiarta I Wayan Sudira I Wayan Wirata I. H. U Utama I.K. Berata Ida Bagus Kade Suardana Ida Bagus Made Oka Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Imam Sobari imam sobari Indahyani, Ni Made Dwi Indra, Rusmin Indrawan, Hieronimus Irhas, Rajiman Iwan Harjono Utama Janardani, Ni Made Kunti Jayawardhita, Anak Agung Gde Jihadulhaq, Jihadulhaq Kadek Karang Agustina Kartika, Erena Hajar Ketut Budiasa Ketut ELI Supartika Ketut Santhia Adhy Putra Ketut Tono Pasek Gelgel Komang Andika Purnama Komang Ariya Hendrayana Kristi Agusti Putri, Kristi Agusti Lagho, Engelbertus Efraim Luh Gde Sri Surya Heryani Luh Made Sudimartini Mahadewi, S. A. Mahadewi, S.A. Mahyuzar Mahyuzar Muhamad Furkam Fadilah Nallakrishna, I P. A. Ni Ketut Suwiti Ni Luh Eka Setiasih Ni Luh Watiniasih Ni Made Rina Yulinta Ni Nyoman Werdi Susari Ni Putu Ariantari Ni Putu Trisna Asih Ni Wayan Apsari Shantika Pratistha Ni Wayan Listyawati Palgunadi Ni Wayan Listyawati Palgunadi Novianti, Arindita Niatazya Noviriolla Maria Nugraha, Elisabeth Yulia Nur Hanifah Septiani, Nur Hanifah Nurrohman, Fahmi Galuh Nurul Faiziah Nurul Faiziah P. A. C. Kusuma P. A. C. Kusuma, P. A. C. P. Sampurna Paranitha, Dewa Ayu Patrisius Yanuaris Lada Salasa, Patrisius Yanuaris Prabandari, Anak Agung Istri Vera Pranatha, Wahid Danang PRANSIKA EKSY YONITA Purnama, Komang Andika Purnata, I Dewa Nyoman Alit Purwani, S. T. D Purwani, S. T. D. Putri Udayani Putu Agus Trisna Kusuma Antara Putu Dede Yudi Utama, Putu Dede Yudi Putu Mira Puspitayani Rasdiyanah . Rasdiyanah . Ratih, Dwi Kusuma Komala Rui Daniel de Carvalho S. T. D Purwani, Sakura, Yoakim Wilson Samiantara, I. K. Samsuri Samsuri Samuel Leonardo Silitonga Sari, Ni Komang Wahyu Centika Septianingsih, Rayni Silaban, Jesiaman Sri Kayati Widyastuti Sri Milfa Sri Milfa STEVEN YOHANES BOGIA TJOKORDA ISTRI AGUNG Cintya Dalem Tri A. Putra, Tri A. Tri Adi Putra, Tri Adi Wahyu Semadi Putra Widiarta, Lutfi Widyasanti, Ni Wayan Helpina Yanti, I G. A. A. D. Yasunobu Matsumoto Yesi Veronica Sitepu Yoga Eka Prasetyo Yoshihiro Hayashi Yuliantari, Ida Ayu Made Yunita Lestyorini Yunita Lestyorini