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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Indonesian Journal of Biotechnology Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner ARSHI Veterinary Letters Wahana Peternakan Jurnal Pusat Inovasi Masyarakat Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Ni Wayan Kurniani Karja
Departemen Klinik Reproduksi Dan Kebidanan, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jln. Agatis, Dramaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary

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Quality of Chilled Ram Semen in Tris Egg Yolk Extender Added with Different Concentrations of Glutamine: Quality of Chilled Ram semen in extender added with glutamine Aisyah Fidela Siregar; Mokhamad Fahrudin; Ni Wayan Kurniani Karja; Wahono Esthi Prasetyaningtyas
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 7, No 2, JULY 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v7i2.22034

Abstract

Glutamine is a non-essential amino acid that can reduce free radicals and act as an antioxidant. This study aims to determine the effect of adding glutamine in egg yolk tris diluent at concentrations of 0 mM (K), 2,5 mM (G2,5), 5 mM (G5), and 7,5 mM (G7,5) on the quality of liquid semen and kept in a refrigerator at 4 °C. Microscopic evaluation was performed immediately after dilution (H0) and every 24 hours until day 5 (H5). The results showed that the G 7,5 mM group could maintain better motility and viability (P < 0,05) than the K, G 2,5, and G 5 groups. The addition of glutamine did not have a significant effect (P> 0.05) in maintaining the MPU of spermatozoa. Glutamine with a concentration of 7.5 mM is the best concentration to maintain the quality of sheep's liquid semen.
Influence of Melatonin as an Antioxidant on the Preservation of Liquid Ram Semen in Tris-Fructose Egg Yolk Diluent Pattikawa, Vapriel Andhika; Karja, Ni Wayan Kurniani; Fahrudin, Mokhamad; Prasetyaningtyas, Wahono Esthi
Acta VETERINARIA Indonesiana Vol. 13 No. 1 (2025): Maret 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.1.52-59

Abstract

Excessive free radicals can induce oxidative stress, leading to the inhibition of phosphorylation processes that supply energy for sperm capacitation and the acrosome reaction in spermatozoa during fertilization. This study investigated the impact of incorporating the antioxidant melatonin into the tris-fructose egg yolk diluent on the quality of ram liquid semen preserved at 4°C. Fresh semen with ≥70% motility was diluted in the tris-fructose egg yolk group (control), tris-fructose egg yolk with 0.5 mM melatonin (TFEY + M0.5), tris-fructose egg yolk with 1 mM melatonin (TFEY + M1), tris-fructose egg yolk with 2 mM melatonin (TFEY + M2). Semen was stored in the refrigerator at 4°C for 5 days (D0−D5). The motility, viability, abnormality, and plasma membrane integrity of the semen were evaluated every 24 hours. The results indicated no significant difference (P>0.05) between the control group and the liquid semen treatment at D0. Liquid semen quality was significantly improved with melatonin treatment compared to the control group. The results of this study indicate that TKTF + M0.5 had the highest motility and viability values ​​and can maintain abnormality values. The TKTF + M2 group had a higher plasma membrane integrity value than the other treatment groups.
Methods for assessing DNA fragmentation in bovine spermatozoa: A Mini-review of Indonesian research Satrio , Faisal Amri; Magfira; Karja , Ni Wayan Kurniani
ARSHI Veterinary Letters Vol. 9 No. 1 (2025): ARSHI Veterinary Letters - February 2025
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.9.1.29-30

Abstract

Artikel ini mengulas metode penelitian dan analisis fragmentasi DNA sperma sapi yang dilakukan di Indonesia. Tinjauan pustaka menggunakan Google Scholar mengidentifikasi 37 artikel oleh penulis Indonesia yang diterbitkan sejak 2018, dengan puncak delapan artikel pada 2023. Teknik utama yang digunakan meliputi pewarnaan Acridine Orange (17 artikel), kit Sperm-Bos-Halomax (12 artikel), pewarnaan Toluidine Blue (7 artikel), dan uji TUNEL (1 artikel), yang masing-masing menunjukkan variasi metodologis. Tinjauan ini memberikan referensi berharga bagi para pemangku kepentingan yang ingin mengembangkan peraturan standar untuk evaluasi sperma pada sapi.
EFEK SUPLEMENTASI MELATONIN PADA MEDIA PENGENCER TERHADAP PARAMETER KUALITAS SPERMA DOMBA PASCA-EKULIBRASI: Effects of Melatonin Supplementation in Dilution Media on Sperm Quality Parameters in Sheep Post-Equilibration Frilianty Putri; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi; Ekayanti Mulyawati Kaiin; Rizky Amrullah Chaniago
Wahana Peternakan Vol. 9 No. 2 (2025): Wahana Peternakan
Publisher : Faculty of Animal Science, University of Tulang Bawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jwputb.v9i2.2687

Abstract

Penelitian ini bertujuan untuk mengevaluasi pengaruh suplementasi melatonin dalam media pengencer terhadap kualitas spermatozoa domba pasca-ekuibrasi. Melatonin dikenal sebagai antioksidan kuat yang mampu menangkal stres oksidatif, terutama yang terjadi selama proses kriopreservasi dan pencairan semen. Parameter yang diamati dalam penelitian ini meliputi motilitas, integritas membran plasma, keutuhan tudung akrosom, dan viabilitas spermatozoa, baik sebelum maupun setelah proses ekuibrasi. Hasil penelitian menunjukkan bahwa penambahan melatonin pada konsentrasi 1 mM memberikan peningkatan yang signifikan terhadap motilitas spermatozoa (83,33 ± 2,8%) dan integritas membran plasma (81,63 ± 0,62%) dibandingkan dengan kelompok kontrol. Selain itu, viabilitas spermatozoa pasca-ekuibrasi juga meningkat secara signifikan pada kelompok perlakuan (76,41 ± 1,4%) dibandingkan dengan kelompok kontrol (71,67 ± 2,2%). Penambahan melatonin dalam media pengencer terbukti mampu mempertahankan kualitas spermatozoa selama proses kriopreservasi. Temuan ini menunjukkan bahwa melatonin memiliki potensi besar sebagai aditif dalam pengencer semen guna meningkatkan efektivitas dan keberhasilan program inseminasi buatan pada ternak, khususnya pada domba.   Kata kunci: Ekuiblirasi, Melatonin, Spermatozoa
-TOCOPHEROL IMPROVED THE QUALITY OF BEAGLE DOG BREED CHILLED SEMEN STORAGE AT 4 C IN TRIS-SKIM MILK EXTENDER Makhroja, Lalu Faris Naufal; Juniantito, Vetnizah; Fahrudin, Mokhamad; Prasetyaningtyas, Wahono Esthi; Karja, Ni Wayan Kurniani
Jurnal Kedokteran Hewan Vol 19, No 3 (2025): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i3.44561

Abstract

Improvement of canine reproduction has been developed by artificial insemination (AI) for effective breeding. -tocopherol widely recognized as an antioxidant that has been used to improve the quality of the spermatozoa. This study evaluated the effect of -tocopherol supplementation in dog chilled semen quality. Beagle dog breed semen samples were obtained from male Beagles using massage technique. The semen was then diluted with tris-skim milk extender supplemented with -tocopherol of various concentration, 0% as control group (T-0), 0.1% (T-0.1), 0.2% (T-0.2), and 0.3% (T-0.3), then were stored at 4 C and evaluated daily for four days, particularly for variables namely sperm motility, sperm viability, and plasma membrane integrity. Results showed that the quality of spermatozoa was higher when spermatozoa preserved in extender containing of 0.1% or 0.2% -tocopherol compared to those of control and 0.3% -tocopherol group (P0.05). The highest quality found in group with 0.2% -tocopherol. In conclusion, supplementation -tocopherol 0.2% in dog chilled semen extender is effective for improving and maintaining chilled dog sperm quality for 4 days of storage.
Fresh Semen Characteristics, Biochemical and Mineral Composition of Seminal Plasma from Different Age Groups of Balinese Cattle Witri, Brilla Widya; Arifiantini, Iis; Prasetyaningtyas, Wahono Esthi; Karja, Ni Wayan Kurniani
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.273-285

Abstract

Age is among the factors affecting reproductive organs, particularly semen characteristics. Semen characteristics are also influenced by composition of seminal plasma that is ejaculated with the sperm. Therefore, this study aimed to investigate characteristics of fresh semen and seminal plasma composition from Balinese cattle aged 3, 8, and 13 years. Semen characteristics observed were concentration, progressive motility, viability, plasma membrane integrity, acrosome integrity, and sperm abnormalities. Biochemical composition of seminal plasma analyzed was fructose, malondialdehyde (MDA), total antioxidant capacity (TAC), glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), total protein (TP), albumin, cholesterol, and triglycerides. Mineral compositions observed were Na, K, Ca, Mg, Zn, Cu, Fe, and Se. The results showed that there were no significant differences (p > 0.05) in semen characteristics across age groups, meaning that semen of Balinese cattle can still be processed into frozen semen even at an older age, provided it meets the SNI 4869-1:2024 standards. Furthermore, the concentrations of each seminal plasma biochemical component were as follows: fructose (377.5–615.4 mg/dL), MDA (9.7–10.36 nmol/mL), TAC (66.22–67.22%), GOT (203–265 U/L), GPT (19–35 U/L), TP (4.3–5.5 g/dL), albumin (1.7–1.9 g/dL), cholesterol (11–35 mg/dL), and triglycerides (822–1.078 mg/dL). Surprisingly, the concentration of triglycerides in semen plasma in this study was very high compared to those reported in previous studies on several cattle breeds. Seminal plasma mineral concentrations included Na (1.500–1.900 mg/kg), K (600–700 mg/kg), Ca (600–800 mg/kg), Mg (38.85–45.16 mg/kg), Zn (5.33–9.64 mg/kg), Cu (0.06–0.12 mg/kg), Fe (11.85–15.88 mg/kg), and Se (0.92–1.48 mg/kg). These findings indicate that age did not affect semen characteristics, biochemical, and mineral composition of seminal plasma in Balinese cattle.
THE QUALITY OF CHILLED RAM SEMEN AFTER STORAGE IN EXTENDER WITH ADDITION OF MELATONIN AT 4 C Triyaningrum, Triyaningrum; Fahrudin, Mokhamad; Karja, Ni Wayan Kurniani; Prasetyaningtyas, Wahono Esthi
Jurnal Kedokteran Hewan Vol 18, No 1 (2024): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i1.34649

Abstract

Semen was collected using an artificial vagina, and then examined macroscopically and microscopically. Semen with motility of 70% was diluted into different extenders: tris egg yolk-glucose (TEYG), tris egg yolk-glucose extender with 0.5 mM melatonin (TEYG + M0.5), tris egg yolk-glucose extender with 1 mM melatonin (TEYG + M1), tris egg yolk-glucose extender with 2 mM melatonin (TEYG + M2). All semen samples were stored at 4 C for 5 days (H0-H5) and examined every 24 hours. Semen was examined for spermatozoa motility, viability, abnormality, and membrane plasma integrity. The results showed that there were no significant changes of semen quality on H0 (P0.05). In general, the melatonin supplemented groups had a higher semen quality value than the control. Melatonin supplementation can protect and maintain the quality of chilled semen. In addition, the result showed that TEYG + M1 extender was the best extender treatment, which was indicated by higher values than other treatments.
PRODUCTIVITY AND FRESH SEMEN CHARACTERISTICS OF SIMMENTAL BULL DIFFERENT AGES Satrio, Faisal Amri; Karja, Ni Wayan Kurniani; Setiadi, Mohamad Agus; Kaiin, Ekayanti Mulyawati; Kurnia, Asep; Purwantara, Bambang
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i1.23487

Abstract

This study aimed to determine the effect of age on bulls productivity and fresh semen characteristics of Simmental bull in Indonesia. A total of 1071 data of semen collection and production from four age groups (two years old (yo), four yo, 10 yo with high semen rejection (10 HR), and 10 yo with low semen rejection (10 LR) were used in this study to evaluate the productivity and characteristics of fresh semen. The results showed that the pre-freezing and post-freezing semen rejection rate of 10 HR group was higher (P0.05) than the other groups. The four yo group had the percentage of second semen ejaculation each collection was higher (P0.05) than the other groups. Furthermore, semen volume, sperm concentration, and total sperm concentration significantly increased (P0.05) until four yo and then decreased (P0.05) in 10 yo groups. The 10 HR group had the volume and total sperm concentration significantly different (P0.05) with a group of 10 LR. Total sperm motility, individual motility, and mass movement were lower (P0.05) in 10 HR than the other groups. In conclusion, age differences of bulls can affect the productivity and characteristics of fresh semen.
THE EFFECTIVITY OF CYSTEAMINE SUPPLEMENTATION ON IMPROVING THE IN VITRO FERTILIZATION RATE OF SHEEP OOCYTES Nurkarimah, Dona Astari; Kaiin, Ekayanti Mulyawati; Karja, Ni Wayan Kurniani; Setiadi, Mohamad Agus
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i4.17688

Abstract

The purpose of this study was to evaluate the effectiveness of cysteamine supplementation on the maturation medium and/or in vitro fertilization medium with regards to improving the normal fertilization rate of sheep oocytes, which are characterized by the formation of two pronuclei. Grade A and B oocytes were matured in medium-199 with 0.3% bovine serum albumin (BSA), 10 IU/mL follicle stimulating hormone (FSH), 10 IU/mL human chorionic gonadotrophin (hCG), and 50 g/mL gentamicin added for 24 hours in a 5% CO2 incubator at 39 C. The treatment group was divided into the following groups: a control group with no cysteamine (P1), a group with 100 M cysteamine in the maturation medium (P2), a group with 100 M cysteamine in the fertilization medium (P3), and a group with 100 M cysteamine both in the maturation and fertilization medium (P4). The fertilization was carried out by incubating sperm-oocytes for 12 hours and then staining them with 1% aceto-orcein to observe the formation of a pronucleus. Normal fertilization rates obtained by each treatment group were 56.5% (P1), 57.1% (P2), 57.8% (P3), and 59.9% (P4) with no significant difference (P0.05) between groups. It was concluded that 100 M cysteamine supplementation in both the maturation medium and fertilization medium was not able to increase the normal fertilization rate of sheep oocytes.
QUALITY OF CHILLED CANINE SEMEN IN TRIS-EGG YOLK EXTENDER SUPPLEMENTED WITH SERICIN Khye, Kim Chwin; Yusuf, Tuty Laswardi; Satrio, Faisal Amri; Karja, Ni Wayan Kurniani
Jurnal Kedokteran Hewan Vol 15, No 1 (2021): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i1.17641

Abstract

ABSTRACTThe objective of this research was to evaluate the quality of chilled canine semen in Tris-egg yolk (TEY) extenders containing different concentrations of sericin. Semen were collected from four dogs by massage method. Canine semen was collected using sterile urine pots and evaluated. Sperm-rich fractions were pooled and divided into four equal aliquots, which were then diluted with TEY extenders supplemented with different concentrations of sericin (0%, 0.1%, 0.25%, 0.5%). The diluted semen aliquots were preserved at 4 in sterile centrifuge tubes and were then evaluated for spermatozoa motility, viability, plasma membrane integrity and acrosome integrity every 12 hours up to 72 h. The TEY extenders supplemented with 0.25% and 0.5% sericin resulted in higher spermatozoa motility and viability at 72 h compared to other TEY extenders (P0.05). The integrity of plasma membrane and acrosome of spermatozoa showed no significant differences among the groups extenders at 72 h. In conclusion, sericin in concentration of 0.25% and 0.5% were able to prevent the motility and viability of canine spermatozoa after storage for 72 h.
Co-Authors . Hasbi Abdullah Baharun Achmad Setiyono Adi Winarto Aisyah Fidela Siregar Alvien Nur Aini Amrozi Ananda Ananda Andhani Widya Hartanti Andriani Andriani Andriani Andriani Anita Hafid Arie Febretrisiana Arie Febretrisiana, Arie Asep Kurnia Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Bambang Purwantara Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bayu Sulistyo Bq Hayyul Hidayati Cece Sumantri Damanik, Asman Ramadhan Dhia Mardhia Engcong Diana Andrianita Kusumaningrum Dwitya Citraesti Edelina Sinaga Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Muyawati Kaiin Elmanaviean, Muhammad Faisal Amri Satrio Feni Dwi Kartika Gulo Frilianty Putri Frilianty Putri Gusdinar, Rizal Gusman, Khalis Talitha Gustina, Sri Hafizuddin Hafizuddin Hariono Hasbi Hasbi Ian Kurniawan Iman Supriatna Indriastuti, Rhesti Kaiin, Ekayanti Mulyawati Kazuhiro Kikuchi Kazuhiro Kikuchi KAZUHIRO KIKUCHI Ketut Adnyane Mudite Khye, Kim Chwin Kim Chwin Khye Kurnia, Asep Kusdiantoro Mohamad Latifah Humairoh Lisa Dwi Fannessia Lisa Praharani M Agus Setiadi M Imron M. Elmanaviean Magfira Magfira Magfira Maghfira Maghfira Makhroja, Lalu Faris Naufal Masir, Ummul Masturi Muhajir Memili, Erdogan Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad MUHAMMAD AGIL Muttaqinullah Rabusin Neta Fitria Yasa Nugraha, Arifin Budiman Nurkarimah, Dona Astari Nur’aisyah Amrah Safitri Okky Adi Bintara Oloan Parlindungan Pardede , Berlin Pandapotan Pardede, Berlin Pandapotan Pattikawa, Vapriel Andhika Praharani, Lisa Puwantara, Bambang R. Iis Arifiantini Reski Adelia Rizal Gusdinar Rizky Amrullah Chaniago Setiadi , Mohamad Agus Silvia Anggraini Siska Adelya Ramadhani Sri Estuningsih Sri Gustari Srihadi Agungpriyono Surya Agus Prihatno Surya Agus Prihatno Syafri Nanda Syahruddin Said Tatsuyuki Suzuki Tatsuyuki Suzuki, Tatsuyuki Tike Sartika Tike Sartika Titis Prastiwi Triyaningrum, Triyaningrum Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Veronika Gilang Pravitasari Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Widyasanti, Ni Wayan Helpina Winny Plumeria Aqshani Witri, Brilla Widya Yesi Pratiwi Kusumawati Yuke Rizky Amelia Yulida Nofa Yuni Siswani Zultinur Muttaqin