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All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Indonesian Journal of Biotechnology Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner ARSHI Veterinary Letters Wahana Peternakan Jurnal Pusat Inovasi Masyarakat Jurnal Kedokteran Hewan Policy Brief Pertanian, Kelautan, dan Biosains Tropika Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia

Ni Wayan Kurniani Karja

Departemen Klinik Reproduksi Dan Kebidanan, Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Jln. Agatis, Dramaga, Bogor 16680

Author-ID : 295831

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary

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Kompetensi Maturasi Oosit in vitro dan Kajian Histologi Folikel dari Ovarium Domba Pascapenyimpanan pada Suhu 4°C Masturi Muhajir; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi; I Ketut Mudite Adnyane
Acta VETERINARIA Indonesiana Vol. 6 No. 2 (2018): Juli 2018
Publisher : IPB University

Penelitian ini bertujuan untuk mengkaji kompetensi maturasi oosit secara in vitro dan gambaran histologi ovarium pascapenyimpanan ovarium pada suhu 4°C selama empat hari. Ovarium dari rumah potong hewan dibagi menjadi 4 kelompok dan disimpan pada suhu 4°C selama 0 jam (kelompok H-0/kontrol), 24 jam (Kelompok H-1), 48 jam (Kelompok H-2), 72 jam (Kelompok H-3) dan 96 jam (Kelompok H4). Pada setiap akhir periode penyimpanan, oosit dikoleksi dan diseleksi berdasarkan keadaaan kekompakan sel-sel kumulus, kehomogenan dari sitoplasma (Grade A sampai C). Oosit dengan grade A dan B dimaturasi secara in vitro selama 24 jam. Gambaran folikel dalam ovarium pascapenyimpanan dikaji dengan pewarnaan Hematoksilin-Eosin. Terjadi penurunan yang signifikan (P<0.05) pada jumlah oosit dengan grade A setelah hari kedua penyimpanan. Kemampuan oosit untuk mencapai MII menurun setelah penyimpanan hari kedua (P<0.05). Seiring dengan penurunan jumlah oosit yang mencapai MII, terjadi peningkatan jumlah oosit yang mengalami degenerasi pada hari ketiga dan keempat pascapenyimpanan ovarium (P<0.05). Dari gambaran histologi, ditemukan adanya folikel yang mengalami piknotik setelah penyimpanan 24 jam. Berdasarkan hasil penelitian dapat disimpulkan bahwa kualitas oosit persentase oosit mencapai MII menurun setelah penyimpanan 24 jam. Terjadi perubahan struktur sel dan degenerasi dari oosit pada gambaran histologi folikel dalam ovarium.

Karakteristik Semen dan Korelasi antara Konsentrasi Testosteron dengan Libido Pejantan Sapi Simental Abdullah Baharun; Syahruddin Said; R. Iis Arifiantini; Ni Wayan Kurniani Karja
Acta VETERINARIA Indonesiana Vol. 10 No. 1 (2022): Maret 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.1.1-7

Penelitian ini bertujuan untuk mempelajari karakteristik semen dan mengevaluasi hubungan antara konsentrasi testosteron dengan libido pejantan sapi Simental. Sebanyak 12 ekor sapi Simental, 9 ekor dengan motilitas spermatozoa ≥70% (A) dan 3 ekor dengan motilitas spermatozoa <70% (B) berdasarkan data sekunder dari Balai Inseminasi Buatan (BIB) tahun 2018-2019. Semen ditampung menggunakan vagina buatan pada pagi hari. Evaluasi kualitas semen segar hanya dilakukan dengan penilaian morfologi spermatozoa. Sampel darah disentrifugasi (3000 rpm, 10 menit), supernatan dimasukkan ke dalam microtube dan disimpan pada suhu -20 °C. Analisis testosteron menggunakan metode ELISA. Data kualitas semen segar sapi pejantan Simental dianalisis secara deskriptif. Korelasi antara karakteristik semen, libido dan konsentrasi hormon testosteron menggunakan korelasi Pearson’s. Hasil analisis menunjukkan terdapat perbedaan signifikan (P<0,05) antara kualitas semen segar kelompok pejantan A dengan pejantan B dalam parameter motilitas, konsentrasi, dan morfologi spermatozoa normal. Volume semen dan skor libido pada semua pejantan tidak menunjukkan perbedaan signifikan. Konsentrasi testosteron pada kelompok pejantan A lebih tinggi (42,57 ng/mL) dibandingkan dengan kelompok pejantan B (33,26 ng/mL). Konsentrasi testosteron menunjukkan korelasi positif (P<0,01) dengan karakteristik semen seperti motilitas spermatozoa (0,813), morfologi spermatozoa normal (0,639), dan libido (0,952). Hasil penelitian ini dapat digunakan sebagai pertimbangan untuk dilakukannya pengukuran konsentrasi testosteron dalam proses seleksi sapi jantan menjadi pejantan.

PCS-4 In Vivo Embryo Production at Cipelang Livestock Embryo Centre Faisal Amri Satrio; Ni Wayan Kurniani Karja; M Imron; Yuni Siswani; Oloan Parlindungan; Bambang Purwantara
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (514.888 KB)

Livestock Embryo Center/Balai Embrio Ternak (BET) Cipelang is one of the government institution under the supervision of Directorate General of Livestock and Animal Health Services, Ministry of Agriculture of the Republic of Indonesia. BET Cipelang has the main task of carrying out the production, development, and distribution of livestock embryo for all regions of Indonesia. BET Cipelang produced embryos using two methods, there are in vivo and in vitro. The produced embryos will be transferred to the recipient cow using embryo transfer technique. Embryo transfer biotechnology can be used to enhance genetic improvement and to increase marketing opportunities with purebred cattle. Because of their relatively low reproductive rate and long generation interval, embryo transfer is especially useful with cattle. The success of embryo transfer depends on factors associated with the embryo, the recipient or an interaction among the factors of the embryo and recipient [1]. Embryo quality is one of the main factors for successful embryo production. In vivo embryo production has better quality than in vitro such as cell number, morphology, growth and development ability and after freezing. The frequency of occurrence of chromosomal abnormalities in in vivo-derived embryos are lower than in vitro and the consequence is that only 30-40% of oocytes resulting from in vitro maturation develop into blastocysts after in vitro fertilization and the in vitro yield rate of embryos are lower than from in vivo [2]. Recently, there are no studies about the performance of in vivo embryo production at BET Cipelang. The aim of this study is to explore the quality of in vivo embryos produced by BET Cipelang.

PF-17 The Development of Crude Testicular Cells in In Vitro Culture Wahono Esthi Prasetyaningtyas; Ni Wayan Kurniani Karja; Srihadi Agungpriyono; Mokhamad Fahrudin
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.568 KB)

Spermatogenesis is a continuous process in which spermatogonial stem cells (SSC) develop into specific germ cells before terminally differentiating to form spermatozoa. The process is supported by Sertoli cells, which are in close contact with germ cells in the seminiferous tubules. Sertoli cells provide essential hormonal signals, nutrients, and physical support to germ cells for successful spermatogenesis.The crude testicular cells (CTC) contains many cell types, like Sertoli cell, Leydig cell, spermatogonial stem cell (SSC), spermatocyte and other testicular somatic cells (Shah et all. 2016). Testicular cells are believed to secrete various growth factors that induced the spermatogenesis process. The spermatogonial stem cells are unique population of cells in the male testis, which dual function. First self-renewing their population to maintain the number of stem cells, secondary function is differentiating into spermatids in testis (Wang et al. 2015).Spermatogenic cells differentiation needed the similar microenvironment in vivo spermatogenesis. The essential nutrients was collected from healty culture and the culture contained mixed population of cells both the somatic cells and spermatogenic cells. To identification the spermatogenic cells using Periodic Acid Schifft (PAS) staining (Chang et al. 2011). The present study examined the development of crude testicular cells using PAS staining.

Adiponectin: Potential Protein Hormone as a Candidate Biomarker for Male Fertility H Hafizuddin; Ni Wayan Kurniani Karja; Lisa Praharani; Mohamad Agus Setiadi
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 29, No 4 (2019): December 2019
Publisher : Indonesian Center for Animal Research and Development

Reproduction is an important factor that requires attention to increase livestock production. The application of artificial insemination (AI) technology has been developed rapidly in the world, so that availability of fertile superior male is a determinant factor of successful AI. Several recent studies have been aimed to discover male fertility biomarker, by intense research on fertility-associated proteins contained in seminal plasma. This paper describes the role of adiponectin as a biomarker candidate of male fertility. Adiponectin has a positive effect on spermatozoa functionality and steroidogenesis. This has been proven by several studies that found its expression in Sertoli cells and Leydig cells in the reproductive tract. Based on the specific role on spermatozoa functionality and steroidogenesis in supporting male fertility parameter, it is strongly suggested that adiponectin is an excellent candidate biomarker for male fertility.

Relationship between Nucleus Swelling and Development Competence of Bovine Cloned Embryos Reconstructed by Enucleated Oocytes with Serum-starved or Serum-fed Fetal Somatic Cells Mokhamad Fahrudin; Ni Wayan Kurniani Karja; Tatsuyuki Suzuki
Indonesian Journal of Biotechnology Vol 10, No 2 (2005)
Publisher : Universitas Gadjah Mada

This study was conducted to examine the occurrence of nuclear remodeling (nucleus swelling) and its effects on the subsequent in vitro development of bovine embryos reconstructed by serum-starved and serum-fed somatic cells. Results from this study demonstrated that all of the reconstructed embryos that received serum-starved and serum-fed somatic cells exhibited condensed-nuclei. More than 90% of the transferred nuclei exhibited nuclear envelope breakdown and premature chromatin condensation which clearly distinct from an intact nucleus. There was no significant difference on the degree of nucleus swelling in SS-NT embryos or SF-NT embryos, indicating that either serum-starved or confluent somatic cell lines could be reprogrammed by the recipient cytoplasm environments in similar pattern. Although the fusion rate was not significantly different among the groups, the proportion of SS-NT embryos which developed to the 2- to 4-cell stage (89.7%) and to the 8- to 16-cell stage (74.7%) was significantly higher than that of SF-NT embryos. Whereas, the proportion of reconstructed embryos that developed to the morula and blastocyst stages were not significantly different among the groups. Results of these studies demonstrate that reconstructed embryos, which received either serum-starved or serum-fed confluent somatic cells, showed similar developmental competence to the blastocyst stage.

Nuclear Maturation of Porcine Oocytes in vitro: Effect of the Cumulus-Oocyte Complexes Quality Ni Wayan Kurniani Karja
Indonesian Journal of Biotechnology Vol 13, No 2 (2008)
Publisher : Universitas Gadjah Mada

The objective of this study was to examine the effect of the cumulus-oocyte complexes (COCs) quality on the ability of porcine oocytes to mature in vitro. Porcine COCs were collected from 2-6 mm follicles of slaughterhouse ovaries. The oocytes used for IVM were classified into three categories based on the compactness and transparency of the cumulus investment and homogeneity and transparency of the ooplasm. The oocytes were then matured in vitro for 44 h. At 22 of maturation culture, most of the oocytes in all</div><div>groups were identified still at germinal vesicle (GV) stage and metaphase I (M-I) stage. After 44 h of culture, a greater proportion of Category I and II oocytes completed in vitro maturation through the second meiotic as compared with that of Category III oocytes (P<0.05). The proportion of oocytes remaining at M-I stage and the degenerative oocytes in Category III oocytes were significantly higher than those of oocytes in other groups (P<0.05). These data indicate that porcine oocytes with high quality cytoplasm and a cumulus cell complement have a much greater chance of maturing in vitro than that lower quality oocytes. The morphological grading of immature oocytes is an appropriate selection criterion for their developmental ability.

Study of Repeat Breeder of Dairy Cows in Farmer Level Surya Agus Prihatno; Asmarani Kusumawati; Ni Wayan Kurniani Karja; Bambang Sumiarto
Jurnal Sain Veteriner Vol 30, No 2 (2012): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

One of the important reproductive disorders in dairy cows was repeat breeding. Economic losses was quite high, due to the low reproductive efficiency and productivity. Breeder is one of the risk faktors on the incidence of repeat breeding. The aims of this research were to determine the incidence of repeat breeding cases in dairy cattle at Special Regency of Yogyakarta (DIY) and many factors from the farmers and dairy cattle aspects that caused the cases. In this study, 401 traditional farmers at DIY were used. The method of data collection was done by interviewing the farmers and direct observation to the dairy cattle. The data consist of farmer education level, length period of farming experience, stall condition, length distance from inseminator, estrous detection ability,knowledge of estrous, estrous observation by farmer, stall cleanliness, cattle cleanlines, and squeezing time.Data were collected on a descriptive analysis of each variable studied. Analysis used logistic regression models to analyze the faktors that influence the incidence of repeat breeding. The sensitivity and specificity of the models were analyzed by Lemeshow goodness of fit test.The result of this study showed that the prevalence of repeat breeding in dairy cattle from farmer aspect was 29.4%. The factor from farmer aspect which affected repeat breeding cases was estrus detection once per day(coefficient regression 4.09563, OR=60,1), detection of estrus twice daily (+ 2.92553, OR=18.6) , disordersof pregnancy (+2.22630, OR= 9.3), dirty environment (+0.91889, OR=2,5), worst drainage (+2.58072, OR=13,2), and education (+1.31295, OR=3,7). It was concluded that the prevalence of repeat breeding at DIY as big as 29.4%. Risk factors affecting the repeat breeding were estrus detection once per day, detection of estrus twice a day, dirty environment, worst drainage, and education. Keywords : prevalency, repeat breeding, dairy cattle, logistic regression, odds ratio

Kemampuan Fertilisasi Spermatozoa Sexing dan Perkembangan Awal Embrio Secara In Vitro pada Sapi Alvien Nur Aini; Mohamad Agus Setiadi; Ni Wayan Kurniani Karja
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The aim of the present study was to investigate the fertilization ability of bovine oocytes and early bovine embryonic development in vitro, fertilized by frozen X and Y sperm separated by bovine serum albumin (BSA) gradient column. Oocytes were collected from slaughter house ovarian by flushing and slicing technique. Oocytes were than maturated in tissue culture medium (TCM) 199 supplemented with 10 IU/ml pregnant mare’s serum gonadotropin (PMSG), 10 IU/ml human chorionic gonadotropin (hCG) and 10% fetal bovine serum (FBS) for 24 h in 5% CO2 incubator 39oC. Oocytes then fertilized with three kind of different frozen spermatozoa (X,Y and unsexing spermatozoa as control) for 14 h with final concentration 2x106 spermatozoa/mL. Embryos were cultured insynthetic oviductal fluid (SOF) supplemented with essential and non essential amino acid and 0.3% bovine serum albumin (BSA) for 96 h. Results of the experiments revealed that there was no significant difference (P>0.05) in thefertilization ability (49.17%; 51.40%; 53.42%) for X, Y and control group, respectively. No significant difference (P>0.05) in the number of embryos development (47.77%; 48.25%; 54.43%) for X, Y and control group, respectively. Furthermore, only small number of embryos could pass development blockade (23.80%; 26.08%; 23.61%) for X, Y and control spermatozoa with statistically no significant difference (P>0.05). It is concluded that sexed spermatozoa separated by BSA gradient column had comparable fertilization ability with unsexing spermatozoa and had ability to supported early embryonic development.

Pengendalian Folikulogenesis Ovarium dengan Pemberian Ekstrak Biji Kapas Siska Adelya Ramadhani; Iman Supriatna; Ni Wayan Kurniani Karja; Adi Winarto
Jurnal Sain Veteriner Vol 35, No 1 (2017): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Gosipol is a substances contained in extracted cotton seed which is thought to have the antifertility ability therefore it is often used as a herbal contraceptive. The aim of this study were to assess the folliculogenesis in mice after administrated with cottonseed extract. 60 female mice strain DDY which was 14-15 weeks old and 30-35 g body weight were divided into five groups and given cottonseed extract each 0; 1,5; 2,1 and 2,7 g/kg BW for 5, 10, 15, 24, and 24 + 10 days (without cottonseed treatment). At the end of the treatment period, mice was euthanasia to observe follicular development histomorphology (each three mice of each treatment). Mice estrous status were evaluated based on the description of the vaginal smear cells with Giemsa staining. The results showedthat the number of developing follicles was low (P < 0.05) compared with control after 5 days cottonseed extract administration at dose 2,7 g/kg BW that were 23 ± 3,6. At dose 1,5 and 2,1 g/kg BW the number of follicles was low after 24 days that were 25 ± 10,4 and 27 ± 3,5. Recovery effects of follicle number after cottonseed extract administration for 24 days was the best at a dose of 1,5 g/kg BW. Prolonge of estrous cycle occured in mice which were administrated the cottonseed extract of at all dose treatment. In conclusion, although the decrease in the number of developing follicles and prolonge of estrous cycles occurred after cottonseed extract administration, but these effects are reversible after the administration ended.

Co-Authors . Hasbi Abdullah Baharun Achmad Setiyono Adi Winarto Aisyah Fidela Siregar Alvien Nur Aini Amrozi Ananda Ananda Andhani Widya Hartanti Andriani Andriani Andriani Andriani Anita Hafid Arie Febretrisiana Arie Febretrisiana, Arie Asep Kurnia Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Bambang Purwantara Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bayu Sulistyo Bq Hayyul Hidayati Cece Sumantri Damanik, Asman Ramadhan Dhia Mardhia Engcong Diana Andrianita Kusumaningrum Dwitya Citraesti Edelina Sinaga Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Muyawati Kaiin Elmanaviean, Muhammad Faisal Amri Satrio Feni Dwi Kartika Gulo Frilianty Putri Frilianty Putri Gusdinar, Rizal Gusman, Khalis Talitha Gustina, Sri Hafizuddin Hafizuddin Hariono Hasbi Hasbi Ian Kurniawan Iman Supriatna Indriastuti, Rhesti Kaiin, Ekayanti Mulyawati Kazuhiro Kikuchi Kazuhiro Kikuchi KAZUHIRO KIKUCHI Ketut Adnyane Mudite Khye, Kim Chwin Kim Chwin Khye Kurnia, Asep Kusdiantoro Mohamad Latifah Humairoh Lisa Dwi Fannessia Lisa Praharani M Agus Setiadi M Imron M. Elmanaviean Magfira Magfira Magfira Maghfira Maghfira Makhroja, Lalu Faris Naufal Masir, Ummul Masturi Muhajir Memili, Erdogan Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad MUHAMMAD AGIL Muttaqinullah Rabusin Neta Fitria Yasa Nugraha, Arifin Budiman Nurkarimah, Dona Astari Nur’aisyah Amrah Safitri Okky Adi Bintara Oloan Parlindungan Pardede , Berlin Pandapotan Pardede, Berlin Pandapotan Pattikawa, Vapriel Andhika Praharani, Lisa Puwantara, Bambang R. Iis Arifiantini Reski Adelia Rizal Gusdinar Rizky Amrullah Chaniago Setiadi , Mohamad Agus Silvia Anggraini Siska Adelya Ramadhani Sri Estuningsih Sri Gustari Srihadi Agungpriyono Surya Agus Prihatno Surya Agus Prihatno Syafri Nanda Syahruddin Said Tatsuyuki Suzuki Tatsuyuki Suzuki, Tatsuyuki Tike Sartika Tike Sartika Titis Prastiwi Triyaningrum, Triyaningrum Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Veronika Gilang Pravitasari Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Widyasanti, Ni Wayan Helpina Winny Plumeria Aqshani Witri, Brilla Widya Yesi Pratiwi Kusumawati Yuke Rizky Amelia Yulida Nofa Yuni Siswani Zultinur Muttaqin

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