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Karakteristik Frozen-thawed Spermatozoa Domba Garut yang Dikriopreservasi dalam Pengencer yang Mendapat Imbuhan Orvus ES Paste (THE CHARACTERISTIC OF FROZEN-THAWED GARUT RAM SPERMATOZOA CRYOPRESERVED IN EXTENDER SUPPLEMENTED WITH ORVUS ES PASTE) Andhani Widya Hartanti; Ni Wayan Kurniani Karja
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the present study was to investigate the characteristics of frozen-thawed Garut ramsemen when cryopreserved in extender supplemented with Orvus Es Paste (OEP) as surfactant. Ramsemen were collected and evaluated, then cryopreserved in extender supplemented with OEP at differentconcentrations: OEP-0 (without OEP); OEP-0.25%; OEP-0.50%; OEP-0.75%; and OEP-1.00%. Thecharacteristics observed were motility, viability, and Intact Plasma Membrane (IPM). The results showedthat semen which were cryopreserved and supplemented with OEP had a higher motility, viability, andIPM, respectively compared to those without OEP. No significant differences in the characteristics wereobserved within the different OEP concentrations. It can be concluded that supplementation of OEP in theextender could protect the quality of cryopreserved ram spermatozoa.
Kompetensi Maturasi dan Fertilisasi Oosit Domba Prapubertas Secara In Vitro (DEVELOPMENTAL COMPETENCE OF MATURATION AND FERTILIZATION PREPUBERTAL SHEEP OOCYTES IN VITRO) Anita Hafid; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.613 KB) | DOI: 10.19087/jveteriner.2017.18.1.51

Abstract

The objective of this study was to evaluate the maturation and fertilization ability of oocytes frompre-pubertal sheep ovary in vitro. Prepubertal ovary were collected based on the absence of corpus luteum or absence of corpus albicans in both of the ovaries. Oocytes were collected by slicing methode. Only the oocytes which categories of homogeneous cytoplasm and compact cumulus cells were used and it was matured for 24 hours in CO2 incubator with temperature 39oC. Oocytes fertilized in vitro used post thawed spermatozoa with concentration 5x106 and incubated for 12-14 hours. Oocytes were evaluated on number of oocytes reached MII and number of PN formation. Result of the experiment revealed that there was no significant difference in the percentage of MII oocytes after in vitro maturation (89% vs 90,7%, P>0,05) between prepubertal sheep and pubertal sheep. Meanwhile, the fertilization rate was significantly lower (P>0,05) in prepubertal sheep oocytes compared to pubertal sheep oocytes (60% vs 77,7%). The incidence of polispermic fertilization was higher in prepubertal sheep oocytes than pubertal sheep oocytes (21,8% vs7,4%, P>0,05). In conclusion, prepubertal sheep oocytes and pubertal sheep oocytes have similar in vitromaturation ability, even though the ability to be fertilized of the prepubertal sheep oocytes is lower than the pubertal sheep. ABSTRAK Penelitian ini bertujuan untuk mengetahui kemampuan maturasi dan fertilisasi oosit dari ovarium domba prapubertas secara in vitro. Ovarium domba prapubertas dikoleksi berdasarkan ketidakhadiran korpus luteum atau ketidakhadiran korpus albicans pada kedua ovarium. Oosit dikoleksi dengan metode slicing. Hanya oosit dengan sitoplasma yang homogen dan sel kumulus yang kompak yang dipakai dan dimaturasi selama 24 jam dalam inkubator CO2 dengan suhu 39oC. Oosit difertilisasi secara in vitro menggunakan semen beku dengan konsentrasi 5x106 spermatozoa/mL dan diinkubasi selama 12-14 jam. Pengamatan dilakukan terhadap kemampuan oosit mencapai tahap metafase II (MII) dan pembentukan pronukleus (PN). Hasil penelitian menunjukkan status inti oosit pada tahap MII tidak berbeda antara oosit domba prapubertas dan oosit domba pubertas (89% vs 90,7%, P>0,05) setelah dimaturasi secara in vitro. Sementara tingkat fertilisasi oosit domba prapubertas lebih rendah (P>0,05) dibandingkan dengan oosit domba pubertas (60,0% vs 77,7%). Kejadian polispermi pada oosit domba prapubertas cenderung lebih tinggi dibandingkan dengan oosit domba pubertas (21,8% vs 7,4%, P>0,05). Dapat disimpulkan bahwa oosit domba prapubertas memiliki kemampuan maturasi yang sama dengan oosit domba pubertas namun memiliki kemampuan fertilisasi yang lebih rendah.
POLA GERAKAN SPERMA SAPI SETELAH DIINKUBASI SECARA IN VITRO DALAM MEDIA FERTILISASI DENGAN IMBUHAN HEPARIN DAN/ATAU KAFEIN Achmad Setiyono; Ni Wayan Kurniani Karja; Mohammad Agus Setiadi; Ekayanti Mulyawati Kaiin
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Penelitian ini bertujuan untuk mengevaluasi penambahan heparin dan kafein secara tunggal maupun kombinasi terhadap status pola gerakan sperma selama proses kapasitasi in vitro. Semen beku yang sudah di thawing diinkubasi di dalam media fertilisasi saja atau ditambahkan dengan kafein 2 mM, heparin 10 µg/mL, dan kombinasi kafein 2 mM dan heparin 10 µg/mL selama 60 menit. Total motilitas, motilitas progresif dan pola gerakan sperma (VCL; LIN dan ALH) dievaluasi menggunakan CASA. Evaluasi dilakukan sebelum inkubasi atau 0, 15, 30 dan 60 menit setelah inkubasi di suhu 38.5 oC dan 5% CO2. Hasil penelitian menunjukkan VCL sperma tidak berbeda pada setiap kelompok dengan periode waktu yang berbeda (P>0.05), kecuali pada MF-Caf-2. persentase LIN pada MF-Caf-5 ditemukan lebih rendah dibandingkan kelompok lainnya sampai pada menit ke-30 (P<0.05), kemudian pada menit ke-30 nilainya sama dengan MF-Caf-2 (P>0.05). Segera sebelum inkubasi, ALH sperma pada MF-Caf-5 lebih tinggi daripada MF (P<0.05), tetapi tidak berbeda dengan MF-Caf-2 dan MF-Hep-10 (P>0.05). Sperma mengalami hiperaktif motilitas terjadi selama periode inkubasi dengan menambahkan kafein. Total motilitas sperma mulai menurun pada menit ke-30 pada MF dan dan MF-Hep-10 dan pada menit ke-60 pada MF-Caf-2 (P<0.05). Total motilitas sperma antar kelompok perlakuan pada periode waktu yang sama juga ditemukan tidak berbeda (P>0.05), tetapi motilitas progresif pada menit ke-60 lebih tinggi pada MF-Caf-5-Hep-10 jika dibandingkan dengan MF-Caf-2-Hep-10 (P<0.05). Dapat disimpulkan bahwa penambahan kafein secara tunggal atau dikombinasikan dengan heparin dapat menginduksi terjadinya hiperaktivasi dengan tidak terjadinya penurunan motilitas secara signifikan selama 60 menit selama periode inkubasi.
Nanokitosan Efektif Menekan Jumlah Bakteri Gram Negatif dan Gram Positif Penyebab Endometritis pada Sapi Friesian Holstein secara In Vitro Edelina Sinaga; Ni Wayan Kurniani Karja; Andriani Andriani; Amrozi Amrozi
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.062 KB) | DOI: 10.19087/jveteriner.2021.22.2.198

Abstract

This study was conducted to isolate and identify the caused bacteria of endometritis in Frisian Holstein (FH) and assessed the effectiveness of nanochitosan against bacterial endometritis in vitro. Sample was collected from six FH cows with subclinical endometritis was examined by ultrasonography, then was calculated the number of colonies, isolation and identification of bacteria. Nanochitosan was calculated the protein content and made into neutral pH with concentrations of 0,5%, 1% and 2%. The results showed that the highest total plate count in the endometrial fluid was founded at number 626 with 4,7 ± 0,6 Log cells/mL and the lowest was found at number 532 with 3,3 ± 0,8 Log cells/mL. Bacteria in the Endometritis fluid was founded Gram-negative bacteria were Escherichia coli, Pseudomonas aeruginosa, and Klebsiella sp. Furthermore, Gram-positive bacteria were founded Staphylococcus aureus, Bacillus sp. and S. pyogenes. The challenge test of nanochitosan 1% and 2% with E. coli showed insignificant results when compared with positive controls (P>0,05). Nanochitosan with all concentrations can reduced number colonies of P. aeruginosa and Bacillus sp. significantly compared with negative controls (P<0,05). The challenge test of nano chitosan 0,5% and 2% were able to reduced the number of S. aureus and Klebsiella sp. colonies but could not reduce of S. pyogenes. These findings showed treatment of nanochitosan as an antibacterial is able to reduce the number of E. coli, Klebsiella sp. and P. aeruginosa and Bacillus sp. and S. aureus
Karakteristik dan Respons Estrus Domba Setelah Pemberian Progesteron-Controlled Internal Drug Release Selama 12 dan 13 Hari (CHARACTERISTIC AND ESTRUS RESPONSE OF EWE TREATED WITH PROGESTERONE-CIDR 12 AND 13 DAYS INTERVAL) Neta Fitria Yasa; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (444.25 KB) | DOI: 10.19087/jveteriner.2018.19.4.502

Abstract

CIDR is a device to stimulate and controled estrous cycle in livestock. This study aim to investigate the estrus characteristics and responses of ewes CIDR treated for 12 and 13 days interval. CIDR were treated into 16 ewes for 12 days or 13 days interval. The evaluation of clinical sign of estrus, ferning image, vaginal smear sample, and measurement of vaginal mucus resistance with estrus detector, were performed before treatment of CIDR, at 0, 12, 24, 36, 48, and 54 hour after CIDR removal. Ferning image for both group started from 24 hour after CIDR removal with few ferning pattern. 36 hour after CIDR removal, ferning image already clear. 48 hour after CIDR removal, ferning showed the optimum image, and cover all view. 54 hour after removal, ferning started to decrease. The composition of vaginal epithel dominated by superficial cell started at 24 hours after CIDR removal followed by appearance of estrus sign. The mean of estrus mucus resistance in both groups showed high values before estrus then decreased during estrus. The means of electrical resistance value for both group approximately 310Ù to 700Ù before estrus and approximately 210Ù to 290Ù during estrus. The number of ewes shown the sign of estrus were 75% in group of 12 days interval and 100% in group 13 days. The mean of onset estrus for 12 and 13 days interval groups are 28 and 30 hours after CIDR removal respectively. In conclution, the results of this study showed that CIDR treatment for 13 days show better intensity of estrus sign than 12 days interval group.
Penambahan á-tocopherol sebagai Antioksidan pada Pengencer Tris Kuning Telur Spermatozoa Kucing pada Suhu 4°C Titis Prastiwi; Wahono Esthi Prasetyaningtyas; Ni Wayan Kurniani Karja
Jurnal Veteriner Vol 22 No 4 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.128 KB) | DOI: 10.19087/jveteriner.2021.22.4.456

Abstract

Stres oksidatif memiliki efek merugikan pada kualitas semen selama pengolahan semen untuk inseminasi buatan. Antioksidan á-Tocopherol merupakan salah satu antioksidan yang termasuk ke dalam kelompok antioksigen. Antioksidan ini dapat mengurangi aksi radikal bebas dan menurunkan stress oksidatif pada sperma. Penelitian ini bertujuan menguji efektivitas penambahan á-tocopherol pada pengencer tris-kuning telur terhadap kualitas semen cair yang berasal dari kauda epididimis kucing. Kauda epipidimis kucing didapatkan dengan metode kebiri. Kauda epididimis kemudian dipisah dan dicacah untuk mendapatkan spermatozoa kucing. Semen dengan motilitas >70% diencerkan pada kelompok pengencer tris kuning telur (TKT), tris kuning telur dengan á-tocopherol 0,1% (TKT+T-0,1%), tris kuning telur dengan á-tocopherol 0,2% (TKT+T-0,2%), dan tris kuning telur dengan á-tocopherol 0,3% (TKT+T-0,3%). Semen cair disimpan selama 4 hari (H0-H4) pada suhu 4 °C, kemudian dilakukan evaluasi kualitasnya (motilitas, viabilitas, abnormalitas dan membran plasma utuh sperma) setiap 24 jam. Hasil penelitian menunjukkan kualitas semen cair tidak berbeda signifikan pada H0 (P>0,05), tetapi penambahan á-tocopherol secara umum meningkatkan kualitas semen pada H4 pascapreservasi. Berdasarkan hasil penelitian, kelompok pengencer TKT+T-0,1% adalah perlakuan terbaik.
Kemampuan Maturasi dan Fertilisasi Oosit Sapi yang Diseleksi Menggunakan Teknik Pewarnaan Brilliant Cresyl Blue (SELECTING MATURATION AND FERTILIZATION ABILITY OF BOVINE OOCYTES USING BRILLIANT CRESYL BLUE) Zultinur Muttaqin; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to evaluate the use of brilliantcresyl blue (BCB) in selecting potentialbovine oocytes for maturation and fertilization in vitro. Brilliant cresyl blue (BCB) is a dye that can assessintracellular activity of glucose-6-phosphate dehydrogenase (G6PD), a synthesized enzyme in maturedoocytes. Oocytes were exposed to 26 ?M BCB diluted in modified Phosphate Buffer Saline (mPBS, PBS +10% Fetal Bovine Serum) for 90 minutes is 5% CO2 incubator at 390C. The oocytes were classified accordingto their cytoplasm coloration: oocytes with blue cytoplasm (BCB+) and unstained oocytes (BCB-). Oocytesof the control group were incubated shortly after morphological selection without being exposed to BCB.Afterwards, all groups of oocytes (BCB+, BCB-, and control) were matured and fertilized in vitro. Maturedoocytes were those oocytes that reach metaphase II after 24 hours culturing; whereas oocytes showing twoor more pronuclei at 14 hours post incubation were classified as fertilized oocytes. The nuclear maturationrate was significantly (P<0.05) higher in BCB+ group (78.7% ± 0.41) than the BCB- group (33.3% ± 0.13).However, there was no significance difference (P>0.05) between the BCB+ and the control group (77.1% ±0.32). The fertilization rate was significantly higher (P<0.05) in the BCB+ group (30.5% ± 0.04) comparedto the BCB- (13.6%±0.03) and control group (23.6% ± 0.05). In conclusion, BCB staining of bovine oocytesprior in vitro maturation could be used in selecting potential developing oocytes.
Prevalensi dan Faktor Resiko Kawin Berulang pada Sapi Perah pada Tingkat Peternak (PREVALENCE AND RISK FACTORS OF REPEAT BREEDING IN DAIRY COWS AT THE FARMER LEVEL) Surya Agus Prihatno; Asmarani Kusumawati; Ni Wayan Kurniani Karja; Bambang Sumiarto
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Repeat breeding is a syndrome which affected the reproduction and production efficacy of dairy cattle.The cause of this syndrome may be a herd problem or a variety of individual cow problems. This studyaimed to determine the prevalence and factors which contributed in the repeat breeding syndrome in dairycattle farms in Daerah Istimewa Yogyakarta (DIY). A total of 922 dairy cows which owned by 401 farmerswere used in this study. The cows had a normal estrus cycle, have had at least once calved, age 2.5-8 yearsold, in good health condition, raised traditionally by a farmer or communal system. Multistage and clustersampling method were used in this study. The total numbers of sample at each stage was determined by proportional, whereas the total numbers of farmer was determined using “sampel rambang” Data werecollected by interviewing farmers and direct observation at the farm. Data collected were farmer’s education,length of having farm, farm condition, distance from inseminator, farmer’s ability to detect estrus andestrus cycle, the farm and cow’s hygiene. Data were analyzed by descriptive statistics then followed by ChiSquare and Odds Ratio. The results showed that the prevalence of repeat breeder at the dairy farms was29.4%. Risk factors at the herd level were the most common i.e.: estrus detection once per-day (OR = 17.8);estrus detection twice per-day (OR = 7.9); unsightly sewer (OR = 10.0); soil floor of enclosure (OR = 2.6); andthe use of wells or rivers as source of water (OR = 2.0 and OR = 1.8, respectively.
Identifikasi Bakteri dan Efektivitas Antibiotik dalam Pengencer untuk Mengontrol Pertumbuhan Bakteri pada Semen Sapi Friesian Holstein Muttaqinullah Rabusin; Andriani Andriani; Raden Iis Arifiantini; Ni Wayan Kurniani Karja
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.835 KB) | DOI: 10.19087/jveteriner.2019.20.1.140

Abstract

This study was designed to investigate the presence of bacterial species in Friesian Holstein (FH) bovine semen at the time of collection, processing and to assess the efficacy of two types of antibiotics combinations; penicillin and streptomycin (PS) and gentamycin, tylosin, lincomycin and spectinomycin (GTLS) in semen extender on bacterial control and quality of semen. For this purpose, three experiments were conducted. In experiment 1, identification of bacterial content in fresh semen which collected from 5 bovine ejaculates. In experiment 2, identification of bacterial content in skimmilk-eggyolk extender which were prepared in artificial insemination center, Lembang, Bandung. In experiment 3, identification of bacterial content in frozen thawed semen. In the result, some of bacterial species were isolated from the bovine semen. The GTLS combination of antibiotics may be incorporated into a freezing extender or protocol without compromising the post-thawed semen quality of FH bull spermatozoa. Three types of bacteria were found in fresh semen; Klebsiella sp., Micrococcus sp., and Pediococcus sp.. Three types of bacteria were found in semen extender; Enterobacter cloacae, Pseudomonas diminuta and Serratia plymutica. Two types of bacteria were found in frozen semen; Enterobacter cloacae and Serratia plymutica. In conclusion, antibiotics PS and GTLS were effective for controlling the growth of bacteria in frozen semen.
Perkembangan Sel Endometrium Domba setelah Inkubasi dalam Kolagenase dan Dikultur In Vitro dengan Estradiol dan Progesteron Ananda Ananda; Ekayanti Mulyawati Kaiin; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.755 KB) | DOI: 10.19087/jveteriner.2019.20.3.298

Abstract

The aim of the present study was to determine the optimal incubation time of collagenase on concentration, viability, quality of endometrial cell culture, and the role of estradiol and progesterone on ovine endometrial cells proliferation. Endometrium minced was incubated into collagenase with different duration of time: 1 hour, 3 hours, and 6 hours repectively. Cell concentration and viability were calculated after incubation. The quality of cell culture was observed on 1st, 3rd, and 5th day after seeding. The best incubation result was then continued with the addition of the E2 (100 pg/ mL), P4 (100 ng/mL), E2:P4 (100 pg/mL : 10 ng/mL), and E2:P4 (10 pg/mL : 100 ng/mL) into the culture medium. After nine days, cell culture was harvested by trypsinization. Concentration and cell viability were analyzed using analysis of variance, followed by Duncan test. Quality of endometrial cell culture was analyzed descriptively. Results showed that there was a significant decreased in the concentration and cell viability obtained in each treatment of collagenase incubation time and optimum treatment of endometrial cell culture was found at 3 hours. Experiment on culture of endometrial cell revealed that proliferation rate treated by E2 and P4 was better then control (P<0.05). Futhermore, additional of E2 into the culture medium even E2 alone (100 pg/mL or higher E2 combination with P4 (100 pg/mL : 10 ng/mL) showed better proliferation rate than P4 alone (100 ng/mL) or higher P4 combination (10 pg/mL : 100 ng/mL). In conclusion, suplementation of 100 pg/mL of estradiol (E2) could support better proliferation of ovine endometrial cells in vitro.
Co-Authors . Hasbi Abdullah Baharun Achmad Setiyono Adi Winarto Aisyah Fidela Siregar Alvien Nur Aini Amrozi Ananda Ananda Andhani Widya Hartanti Andriani Andriani Andriani Andriani Anita Hafid Arie Febretrisiana Arie Febretrisiana, Arie Asep Kurnia Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Asmarani Kusumawati Bambang Purwantara Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bayu Sulistyo Bq Hayyul Hidayati Cece Sumantri Damanik, Asman Ramadhan Dhia Mardhia Engcong Diana Andrianita Kusumaningrum Dwitya Citraesti Edelina Sinaga Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Mulyawati Kaiin Ekayanti Muyawati Kaiin Elmanaviean, Muhammad Faisal Amri Satrio Feni Dwi Kartika Gulo Frilianty Putri Frilianty Putri Gusdinar, Rizal Gusman, Khalis Talitha Gustina, Sri Hafizuddin Hafizuddin Hariono Hasbi Hasbi Ian Kurniawan Iman Supriatna Indriastuti, Rhesti Kaiin, Ekayanti Mulyawati Kazuhiro Kikuchi Kazuhiro Kikuchi KAZUHIRO KIKUCHI Ketut Adnyane Mudite Khye, Kim Chwin Kim Chwin Khye Kurnia, Asep Kusdiantoro Mohamad Latifah Humairoh Lisa Dwi Fannessia Lisa Praharani M Agus Setiadi M Imron M. Elmanaviean Magfira Magfira Magfira Maghfira Maghfira Makhroja, Lalu Faris Naufal Masir, Ummul Masturi Muhajir Memili, Erdogan Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad MUHAMMAD AGIL Muttaqinullah Rabusin Neta Fitria Yasa Nugraha, Arifin Budiman Nurkarimah, Dona Astari Nur’aisyah Amrah Safitri Okky Adi Bintara Oloan Parlindungan Pardede , Berlin Pandapotan Pardede, Berlin Pandapotan Pattikawa, Vapriel Andhika Praharani, Lisa Puwantara, Bambang R. Iis Arifiantini Reski Adelia Rizal Gusdinar Rizky Amrullah Chaniago Setiadi , Mohamad Agus Silvia Anggraini Siska Adelya Ramadhani Sri Estuningsih Sri Gustari Srihadi Agungpriyono Surya Agus Prihatno Surya Agus Prihatno Syafri Nanda Syahruddin Said Tatsuyuki Suzuki Tatsuyuki Suzuki, Tatsuyuki Tike Sartika Tike Sartika Titis Prastiwi Triyaningrum, Triyaningrum TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Veronika Gilang Pravitasari Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Widyasanti, Ni Wayan Helpina Winny Plumeria Aqshani Witri, Brilla Widya Yesi Pratiwi Kusumawati Yuke Rizky Amelia Yulida Nofa Yuni Siswani Zultinur Muttaqin