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All Journal HAYATI Journal of Biosciences Agrotrop : Journal on Agriculture Science METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Bumi Lestari BIOMA : Jurnal Biologi Makassar Journal of Health Sciences and Medicine Indonesian Biodiversity Journal Indonesian Biodiversity Journal Metamorfosa: Journal of Biological Science Open Access DRIVERset
Retno Kawuri
Program Studi Biologi Fakultas Matematika Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Veterinary

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ISOLASI STREPTOMYCES SPP. PADA KAWASAN HUTAN PROVINSI BALI SERTA UJI DAYA HAMBATNYA TERHADAP LIMA STRAIN DIARRHEAGENIC ESCHERICHIA COLI I WAYAN EKA DHARMAWAN; MADE SUSUN PARWANAYONI; RETNO KAWURI
Jurnal Biologi Udayana Vol 13 No 1 (2009): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

An exploration study of natural resources soil bacteria antibiotic-producer, Streptomyces spp. was done in two steps. The first step was isolation of Streptomyces and the second involved testing their inhibition activities against five strains diarrheagenic Escherichia coli. Soil samples were collected from ten forest areas in Bali. As many as 55 isolates were collected with various macroscopic dan microscopic characters. Most isolates (eight Streptomyces isolates) were collected from forest area in Penulisan, Kintamani (RTK. 20). The diversities of isolates are influenced by environment condition. All Streptomyces isolated were tested against five strains diarrheagenic Escherichia coli to check antibiotic activity for inhibit growth of E. coli. Streptomycine was used as a control. The result showed that the largest inhibition zones of Streptomyces against E. coli strains EHEC, ETEC, EIEC, EPEC and DAEC were produced by Streptomyces PK5 (48,67 ± 0,58 mm), Streptomyces GAA4 (29,00 ± 2,00 mm), Streptomyces GBK3 (42,67 ± 2,08 mm), Streptomyces SkBB5 (29,00 ± 2,65 mm) and Streptomyces GM3 (33,67 ± 3,21 mm) respectively.
IDENTIFICATION OF VIBRIO BACTERIA CAUSED VIBRIOSIS ON FRESHWATER PRAWN LARVAE (Macrobrachium rosenbergii (de Man)) Ni Wayan Desi Bintari; Retno Kawuri; A. A. Gde Raka Dalem
Jurnal Biologi Udayana Vol 20 No 2 (2016): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Freshwater prawn (Macrobrachium rosenbergii (de Man)) farming in Bali needs to be supported by the provisionof healthy prawn larvae. Vibriosis infection can be a limiting factor on larval production system which cause highmass mortality in larvae. Therefore a bacteriological study is very important to identify of Vibrio species whichcan cause vibriosis in freshwater prawn larvae. Screening of Vibrio bacteria carried on larval rearing water atUPT Pembenihan Dinas Kelautan dan Perikanan Provinsi Bali which located in Pesinggahan Village, Klungkung.During study, 5 Vibrio isolates can be characterized (VSP01, VSP02, VSP04, VSP05 and VSP06) which wereisolated as dominant Vibrio bacteria in larval rearing water. Koch postulate test results showed that VSP06 cancause vibriosis in freshwater prawn larvae. The result of identification by BBL Cystal™ Identification Systems andidentification book Bergeys’s Manual of Determinative Bacteriology Ninth Edition (Holt et al., 1994), BL6 wasidentified as Vibrio anguillarum.
TOTAL BAKTERI PADA JAMU TRADISIONAL DI PASAR KEDONGANAN KELURAHAN JIMBARAN KABUPATEN BADUNG PROVINSI BALI Putu Ayu Sukmawati; Meitini W. Proborini; Retno Kawuri
Jurnal Biologi Udayana Vol 16 No 2 (2012): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk mengetahui jenis-jenis cendawan, total koloni bakteri, dan mengetahui keberadaan bakteri pencemar Escherichia coli yang terdapat pada jamu tradisional di Pasar Kedonganan. Sampel diambil dari 4 pedagang jamu, setiap pedagang diambil 4 sampel jamu yaitu jamu beras kencur, sirih, kunyit dan sambiloto. Perhitungan Total koloni cendawan dan total koloni bakteri pada jamu dilakukan dengan menggunakan metode pengenceran dan total koloni bakteri secara statistik dianalisa dengan menggunakan Rancangan Acak Lengkap (RAL). Hasil penelitian menunjukkan bahwa 6 jenis cendawan yaitu: Aspergillus niger, A. flavus, Penicillium citrinum, P. digitatum, P. brevicompactum, dan Acremonium sp ditemukan pada jamu. Total jumlah koloni cendawan tertinggi ditemukan pada jamu beras kencur (107x105 CFU/ml) sedangkan terendah ditemukan pada jamu kunyit (20,5 x 105 CFU/ml). Batas standar kandungan jamur pada makanan yang direkomendasikan oleh Departemen Kesehatan RI adalah sebesar < 104 CFU/ml. Total bakteri tertinggi ditemukan pada jamu beras kencur (267,6x108 CFU/ml), sedangkan terendah ditemukan pada jamu kunyit (39x108CFU/ml). Kandungan bakteri juga melampaui standar dari Departemen Kesehatan RI Keseluruhan jamu telah diuji telah melampaui ambang batas Departemen Kesehatan RI yaitu sebesar < 106 CFU/ml. E.coli di temukan jamu sambiloto, jamu kunyit, jamu beras kencur.  Oleh karena itu kehati-hatian perlu dilakukan jika meminum jamu.
AKTIVITAS ANTIBAKTERI FILTRAT Streptomyces sp. KCM2 TERHADAP MULTIDRUG RESISTANT Acinetobacter baumannii SECARA IN VITRO NI KADEK LOSIANI; RETNO KAWURI; A. A. KETUT DARMADI
Jurnal Biologi Udayana Vol 21 No 1 (2017): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.281 KB) | DOI: 10.24843/JBIOUNUD.2017.vol21.i01.p05

Abstract

Acinetobacter baumannii is one of the pathogen which leads to multidrug resistant (MDR-A baumannii). This pathogen is often causing outbreaks of infections to the patients which are receiving treatment in the intensive care unit in hospital. This study aims to find out the antibacterial activity and the Minimum Inhibitory Concentration (MIC) of Streptomyces sp. KCM2 filtrate in inhibiting the growth of MDR-A baumannii. Streptomyces sp.KCM2 isolates was isolated in rhizosphere of white turmeric (Curcuma mango Val.) (Losiani et al., 2016). MDRA. baumannii isolates were obtained from stock culture in Clinical Microbiology Laboratory Sanglah Hospital in Denpasar. The antibacterial and the MIC test of Streptomyces sp. KCM2 filtrate was using the wells diffusion method. The research design was used Completely Randomized Design (CRD) and the inhibitory zone diameterdata of MIC test were analyzed by Analysis of Varian (ANOVA), and then continued by Duncan Multiple Range Test in significance level 5%. The results of this study showed that the Streptomyces sp. KCM2 filtrate was able inhi biting with diameter zone of 23,44 mm and MIC of filtrate was 4% (v/v) with inhibition zone diameter of 8.77 mm.
PENGARUH pH MEDIA PERTUMBUHAN TERHADAP KETAHANAN DARI Rhizobium sp. PADA TANAH YANG BERSIFAT ASAM Ni Made Widyasari; Retno Kawuri; I Ketut Muksin
Jurnal Biologi Udayana Vol 17 No 2 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Telah dilakukan penelitian yang bertujuan mengetahui ketahanan Rhizobium sp. yang ditumbuhkan pada pH media pertumbuhan 7,0 dan 5,8 serta mengetahui kemampuan hidup Rhizobium sp. pada media tanah asam dengan pH 5,0 dan respon inokulum Rhizobium sp. pada tanaman kedelai secara in vivo. ATR (Acid Tolerance Responce) didapatkan dengan cara menumbuhkan Rhizobium sp. dengan pH media pertumbuhan 7,0 dan ditambahkan dengan media YMB (Yeast Extract Mannitol Broth) dengan pH 5,8 dan pH 7,0, diinkubasi pada suhu 280C selama 24 jam pada shaker. Penghitungan total mikroba dilakukan setiap 2 jam sekali dengan menggunakan platting method. Uji ketahanan Rhizobium sp. pada tanah asam dengan pH 5,0 secara in vitro dengan cara menghitung total bakteri dengan menggunakan platting method setiap hari selama 28 hari. Uji in vivo dilakukan dirumah kaca dengan menggunakan metoda MPN (Most Probable Number). Hasil penelitian menunjukkan Rhizobium sp. yang dikondisikan pertumbuhannya dengan pH 5,8 lebih resisten dan dapat membentuk ATR dibandingkan dengan Rhizobium sp. yang ditumbuhkan pada pH media 7,0. Pada pH 5,8 setelah 10 jam total bakteri 285 CFU/g sedangkan pada pH 7,0 total bakteri 148 CFU/g. Rhizobium sp. mampu hidup pada tanah asam dengan pH 5,0 dan membentuk ATR pada hari ke 6 dengan total bakteri 137 x 104 CFU/g, tetapi respon inokulum Rhizobium sp. pada tanah dengan pH 5,0 tidak terjadi pembentukan nodul pada tanaman kedelai dikarenakan tanaman kedelai mengalami defisiensi unsur hara.
JENIS, STATUS DAN PEMANFAATAN TUMBUHAN JENIS POHON DI DESA ADAT BATURNING, KECAMATAN ABIANSEMAL, KABUPATEN BADUNG, BALI N. P. Adriani Astiti; Retno Kawuri; I K. Ginantra
Bumi Lestari Journal of Environment Vol 8 No 2 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

There were 65 species of trees have been identified in Baturning village, District of Abiansemal, Regency of Badung, Bali, which were spread out around the river bank, cemetery (around Dalem temple), around the settlement, non-irrigated farm (plantation or “tegalan”) and rice field. Thirty eight (38) % out of them were observed to be available around the settlement. Most of trees in Baturning (61 species or 94%) have been used by the local community. The rest were uncultivated. From the use, trees in Baturning most were used for human consumption (24%). On the village has not been found protected species, even though in the village were found 22 species of trees which considered to be rare in Bali, namely: Bayur, Bentenung, Beringin, Cempaka, Ee, Jaka, Jeruk Bali, Juwet, Kelor, Kendal, Klampuak, Lempeni, Lenggung, Mahoni, Mengkudu, Palem Raja, Poh-Pohan, Pule, Sandat, Sentul, Udu, and Wani.
Streptomyces sp. Sebagai Biofungisida Patogen Fusarium oxysporum (Schlecht.) f.sp. lycopersici (Sacc.) Snyd. et Hans. Penyebab Penyakit Layu Pada Tanaman Tomat (Solanum lycopersicum L.) NURI MANDAN SARI; RETNO KAWURI; KHAMDAN KHALIMI
Agrotrop : Journal on Agriculture Science Vol 2 No 2 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

A research was conducted to isolate Streptomyces sp. of soil Udayana University campus in theBukit-Jimbaran, to obtain the most effective Streptomyces sp. which is effective in inhibit the growth ofFusarium oxysporum f.sp. lycopersici, and to test response of tomato plants with Streptomyces sp.culture against Fusarium wilt desease. Implementation phases of the research consisted of isolation andidentification of Streptomyces sp, test the inhibition against F. oxysporum f.sp. lycopersici, and in vivotest used by dyeing the roots of the tomato plant (Solanum lycopersicum) with Fusarium spores andafter 30 seconds the roots were dyeing Streptomyces culture. Furthermore, sterile soil in polybagwatered by Fusarium spores and Streptomyces culture at the same time. Based on morphologicalcharacteristic it found five isolates of Streptomyces sp.. The antagonist test showed Streptomyces sp.1 had ability (75%) against Fusarium, Streptomyces sp 2 (68,3%), Streptomyces sp. 3 (71,6%),Streptomyces sp. 4 (63,3%), and Streptomyces sp. 5 (21,6%). All Streptomyces suppressed thegrowth of Fusarium on tomato plants in glass house (p<0,05). Streptomyces sp.3 suppressed Fusariumwilt disease in tomato from 88% in control to 20%.
Penggunaan Streptomyces sp. Sebagai Biokontrol Penyakit Layu Pada Tanaman Cabai Merah (Capsicum annuum L.) yang Disebabkan Oleh Fusarium oxysporum f.sp. capsici ANINDA OKTAVIA RAHARINI; RETNO KAWURI; KHAMDAN KHALIMI
Agrotrop : Journal on Agriculture Science Vol 2 No 2 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

A research has been conducted to find out Streptomyces bacteria at Bukit Jimbaran, to inhibitionpotency of Streptomyces sp. to pathogenic fungi Fusarium oxysporum f.sp. capsici, and to find outantifungal activity of Streptomyces filtrate to F.oxysporum f.sp. capsici in chili (Capsicum annuumL.) plants. Streptomyces sp. isolation was done by platting method with selective media YMA (ISP4).Identification of Streptomyces sp. used Bergey’s book entitled Manual Determinative Bacteriology.Test inhibition against F.oxysporum f.sp. capsici and in vivo test used by dying the roots of the chili(C.annuum L.) plant with F.oxysporum f.sp. capsici and after 30 seconds the roots were dying withStreptomyces sp. culture, furthermore sterile soil on polybag watered by F.oxysporum f.sp. capsicispore and Streptomyces sp. culture at the same time. The result found five isolates Streptomyces sp.with different morphological. The antagonis test showed Streptomyces sp. 4 had ability (82%) againstFusarium, Streptomyces sp.1 (72%), Streptomyces sp.2 (64%), Streptomyces sp.3 (76%), andStreptomyces sp. 5 (32%). All Streptomyces suppressed the growth of Fusarium on chili plants inglass house (p<0,05). Streptomyces sp.4 suppressed Fusarium wilt disease in chili from 80% in controlto 8%.
THE ESSENTIAL OIL CONTENTS OF JERINGAU (Acorus calamus L.) RHIZOMES AND THEIR ANTIFUNGAL ACTIVITY AGAINST Candida albicans Wiwik Susanah Rita; Retno Kawuri; I Made Dira Swantara
Journal of Health Sciences and Medicine Vol 1 No 1 (2017): JHSM (Febuary 2017)
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463.011 KB) | DOI: 10.24843/JHSM.2017.v01.i01.p09

Abstract

Acorus calamus L. rhizome was trusted having antibacterial activity. This study aimed to identify the compounds in the Acorus Calamus L. rhizomes essential oils and to recognize the antifungal activity of the oils against Candida albicans. The extraction of essential oils from rhizome was carried out by steam distillation technique. Identification of compounds in the oils was conducted by Gas Chromatography- Mass Spectroscopy (GCMS), while the antifungal test against Candida albicans was done by well diffusion method. Extraction of 10 kg of rhizomes produced 16.53 mL essential oil with a yield of 0.1653% (? = 1.066), the oil was brownish yellow and very flavorful. GC-MS analysis showed that the essential oil contained 11 compounds, they are (E)-3,7 dimethyl-1,3,6-Octatriene (trans-?-Ocimene) (3,73%), linalool (1,07%), ?-elemene (1,15%), trans methyl isoeugenol (7,68%), shyobunon (15,74%), bicyclogermakren (0,93%), dehidroxy-isocalamendiol (2,61%), ?-calacorene (3,34%), euasarone (26,84), cis-asarone (18,62%); dan trans- asarone (18,29%). Antifungal activity test showed that the growth and biomass inhibition of C. albicans increased with the increase of the oil concentration. Minimum Inhibitory Concentration (MIC) of essential oil toward C. albicans was 1% with the inhibition of 7.83 mm.
Potensi Bakteri Sebagai Biodegradasi Lemak Dan Minyak Pada Lingkungan Yang Tercemar Limbah Domestik Retno Kawuri; Ida Bagus Gede Darmayasa
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p18

Abstract

The contamination of waste oil and grease to the aquatic environment from industrial food processing, restaurants and kitchens or by accidental oil spills is increasingly being found. Biodegradation using a consortium of bacteria can be an effective way to overcome this. The aim of this research was to isolate and identify bacterial strains from waste oil and grease that are capable of biodegradation and the potential of degrading bacteria to degrade oil and grease in vitro. Samples were taken from waste oils and fats. Enrichment techniques were carried out for all samples followed by isolation of bacterial strains to determine which strains could degrade oil and fat in vitro. Identification of bacteria using the identification kit BBL Crystal System. The total bacteria count was carried out using the dilution method. The results showed that there were 3 strains of oil and fat degrading bacteria, namely Bacillus licheneformis, Bacillus coagulant and Psedomonas diminuta. The consortium of the three types of bacteria was able to degrade waste oil and fat with a total of 624x105 CFU / ml to 816x105 CFU / ml bacteria with a control without the addition of a bacterial consortium which was 56x101 CFU / ml. The total bacteria in various treatment wastes with a consortium of bacteria based on molasses in the invitro test showed the ability to live and develop from a fairly high bacterial consortium, namely 292x104 CFU / ml to 904x104 CFU / ml. The bacteria found had the ability to degrade working oils and fats. synergistically. The benefit of this research is that bacteria that have been found can be used as a starter for processing waste fats and oils in environments contaminated with domestic waste fats and oils.
Co-Authors Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Andreas Putro Ragil Santoso, Andreas Putro Ragil ANINDA OKTAVIA RAHARINI Aryadi Millenia Saputra Bayu Putri Handayani Chandrika, Carmen Gisela Darmadi, A.A. Ketut Diah Kharismawati Djereng Dwi Ariani Yulihastuti Felisitas Meli Podhi Gusti Ngurah Alit Susanta Wirya Gusti Ngurah Wisnanda Putra H. Yuswanti I Gede Agus Pradana Putra I Gusti Agung Ayu Ratnawati I Ketut Ginantra I KETUT MUKSIN I Komang Adi Widyastama I Made Dira Swantara I Made Sara Wijana I Putu Gede Ardhana I WAYAN EKA DHARMAWAN I Wayan Yogi Widyastana I.G.A. Gunadi I.G.A.S. Wahyuni Ida Ayu Putri Darmawati Ida Bagus Gede Darmayasa Ilham . Inna Narayani IRIANI SETYAWATI Ivani Dayanara Job Nico Subagio K.A. Yuliadhi K.B. Susrusa Kadek Aryndha Sukma Prabaswari Khamdan Khalimi Khotima Dwi Cahya Luh Made Ary Somia Vagestini LUH Puru ESWARYANTI KUSUMA YUNI Made Mega Yuliasari Made Mira Pratiwi MADE PHARMAWATI Made Ria Defiani MADE SUSUN PARWANAYONI Meitini Proborini Wahyuni Muhammad Inas Riandi N.N.A. Mayadewi NI KADEK LOSIANI Ni Ketut Febri Antini Ni Ketut Yuliana Sari Ni LUH ARPIWI Ni Luh Cipta Ayumi Nellawati Ni Luh Suriani Ni Luh Watiniasih Ni Made Suartini Ni Made Widyasari Ni Nyoman Wirasiti Ni Putu Adriani Astiti NI Putu Diah Septiani Putri Ni Putu Niti Rahayu Ni Wayan Desi Bintari Ni Wayan Purni wirathi Ni Wayan Sudatri NURI MANDAN SARI Putri Permata Putu Anjarina Putu Ayu Parwati Putu Ayu Sukmawati Putu Cindy Arista Revi Juniar Nutrihidayah Rindang Dwiyani Risha Masfufah Risky Vidika Apriyanthi Rochmalia Juniarti Putri Rochmalia Juniarti Putri SANG KETUT SUDIRGA SARMILA TASNIM Suta Arta TRISNA AGUNG PHABIOLA WIWIK SUSANAH RITA Y. Fitriani Yan Ramona