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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Pustakawan Indonesia Agrikultura BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Hortikultura JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Informatika Pertanian Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Pustakawan Indonesia JURNAL ECONOMINA

Kikin H Mutaqin

Institut Pertanian Bogor

Author-ID : 298800

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Immunology & microbiology Library & Information Science Medicine & Pharmacology Social Sciences Veterinary

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Mekanisme Pengendalian Penyakit Busuk Batang Jeruk oleh Khamir, Kitosan, Cendawan Mikoriza Arbuskular, dan Bakteri Simbiotiknya Khairani, Hagia Sophia; Sinaga, Meity Suradji; Mutaqin, Kikin Hamzah
Jurnal Fitopatologi Indonesia Vol. 13 No. 1 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Plant diseases become an important constraint on citrus production. Stem rot disease caused by Botryodiplodia theobromae is a major disease on citrus in Indonesia. This study was aimed to evaluate the mechanism of yeast, arbuscular mychorrhiza fungi (AMF), symbiotic bacteria of AMF and chitosan in controlling stem rot disease. In vitro study was performed to evaluate the mechanism of antibiosis, hyperparasitism, production of volatile compounds, and production of chitinase enzyme. The experiment was continued by in planta assays using yeast, AMF, symbiotic bacteria of AMF, and chitosan either singly or in combination. The experiments were performed using completely randomized design with 3 replications. Disease progress were observed based on its latent period, AUDPC, infection rate, AMF association rate, and total phenol content. In vitro studies indicated that the yeasts showed hyperparasitism to B. theobromae with affinity of 26 cells per hyphae, produced volatile compounds with relative resistance level (RRL) 29.1%, and produced chitinase. Selected symbiotic bacteria from AMF showed antibiosis with RRL 42.9%, production of volatile compounds with RRL 26.7%, and has 98% homology with Bacillus subtilis from Vietnam. Application of yeast + AMF + chitosan delayed disease latent period and suppressed disease incidence. Single AMF and combination of yeast + AMF suppressed disease severity, infection rate, and AUDPC. Application of yeast + AMF showed highest association level of AMF and total phenol content. Therefore, the application of AMF and yeast + AMF is recommended in controlling citrus stem rot disease.

Cara Preservasi Fitoplasma dari Jaringan Kacang Tanah Bergejala Sapu untuk Deteksi DNA dengan Teknik PCR Pulogu, Siska Irhamnawati; Mutaqin, Kikin Hamzah; Giyanto, Giyanto
Jurnal Fitopatologi Indonesia Vol. 13 No. 2 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Witches‘ broom of peanut caused by phytoplasma is a common disease found in Indonesia. Phytoplasma is able to be detected using polymerase chain reaction (PCR) technique. One of important factor which determine the successful of phytoplasma amplification is the DNA availability from fresh tissues. The research was aimed to evaluate some preservation methods of phytoplasma from infected plant samples. The aspects to be evaluated consisted of time (1, 2, 3, and 4 weeks), temperature (-20 °C, 4 °C, and 25 °C), and preservation medium (1X PGB buffer, 3 M NaCl, CTAB buffer, 70% ethanol, non medium, and FTA-card) for storing the fresh phytoplasma infected samples. Good preservation method will optimize the phytoplasma DNA amplification using PCR standard technique followed by nested-PCR. The results showed that preservation of samples at -20 °C, 4 °C, and 25 °C in CTAB buffer was able to maintain the tissue freshness for 4 weeks and was able to provide the DNA of either quality or quantity sufficiently for PCR detection. PCR standard using a primer pair P1/P7 showed that not all of the preserved DNA of phytoplasma were amplified positively. However, standard PCR followed by nested-PCR using primer pair fU5/rU3 was able to increase the DNA detectability. Preserved samples derived from various medium and stored for 4 weeks gave positive results. This results were in contrary with previous same samples which were detected negatively by standard PCR technique.

Galur Isogenik Xanthomonas oryzae pv. oryzae Hasil Penyinaran Ultraviolet dan Potensinya Sebagai Penginduksi Resistensi Padi terhadap Penyakit Hawar Daun Bakteri Yuliani, Fitria; Giyanto, Giyanto; Mutaqin, Kikin Hamzah
Jurnal Fitopatologi Indonesia Vol. 13 No. 2 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is an important rice disease and may cause 50% plant damage. One approach to manage this disease is using isogenic non-pathogenic strain that can induce plant resistance. This study was conducted to obtain isogenic strains of X. oryzae pv. oryzae through ultraviolet irradiation and evaluate ability of the isolates in inducing plant resistance. Bacteria X. oryzae pv. oryzae were exposed to UV for 3, 5, 10 and 15 min. A total of 99 colonies obtained were selected through pathogenicity tests, followed by confirmation using XOR-F and XOR-R2 primers. As much as 16 isogenic strain isolates were obtained with the ability to produce exopolysaccharides (EPS) ranges between 1.4–9.8 mg mL-1 compared to wild-type 6.0 mg mL-1. Five isogenic strains isolates (M313, M57, M101, M1513 and M1515) caused virulence reduction of 92.8–98.6%. Application of those five isogenic isolates prolonged incubation period of X. oryzae pv. Oryzae, i.e. 6.6–5.4 days compared to the wild-type i.e. 4.8 days after inoculation. Suppression of disease progression was also observed with AUDPC value 615.0-827.5, which was lower than those caused by wild-type and control, i.e. 863.3 and 682.5, respectively. Application of isogenic strain isolates did not affect plant growth but it increased plant productivity. Isogenic strain M101 was the best isolate with an incubation period of 5.7 days after inoculation, having the lowest AUDPC value (615.0), and yielded dry weight of 100 grains higher (2.8 g) than those of the wild-type (2.0 g).

Identifikasi Patotipe Xanthomonas oryzae pv. oryzae dari Tanaman Padi di Sulawesi Selatan Asysyuura, Asysyuura; Nawangsih, Abdjad Asih; Mutaqin, Kikin Hamzah; Sudir, Sudir
Jurnal Fitopatologi Indonesia Vol. 13 No. 3 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Xanthomonas oryzae pv. oryzae is the causal agent of bacterial leaf blight (BLB), one of important constraint in rice production. The pathogen is known to have many pathotypes which caused difficulties in disease control. This research was conducted to determine the distribution of X. oryzae pv. oryzae’s pathotypes in seven districts in South Sulawesi. Grouping of pathotypes was performed according to Kozaka method, i.e. based on the response of differential rice varieties. Infection of X. oryzae pv. oryzae was confirmed by PCR using specific primers XOR-R2/XOR-F. Out of 36 isolates, 29 isolates were identified as X. oryzae pv. oryzae which belong to pathotype III (6 isolates), pathotype IV (21 isolates), and pathotype XII (2 isolates). This result indicated that X. oryzae pv. oryzae pathotype IV was distributed widely in South Sulawesi.

Deteksi dan Identifikasi Fitoplasma yang Berasosiasi dengan Penyakit Layu Kelapa Di Pulau Derawan, Kalimantan Timur Prasetyo, Agus Eko; Mutaqin, Kikin Hamzah; Giyanto, Giyanto
Jurnal Fitopatologi Indonesia Vol. 13 No. 3 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Coconut is a major commodity in Derawan island as source of additional income for the farmers. Research was conducted to detect and identify phytoplasmas associated with coconut wilt disease in Derawan island. Coconut wilt disease was indicated by the typical symptoms, i.e. leaf yellowing, shorten of coconut sheaths and leaves, necrosis and collapse of old leaves, and also nut fall. The presence of phytoplasmas in phloem tissues of coconut stem was observed using fluorescence and electron microscope. Identification of phytoplasmas was carried out by nested-PCR and sequencing of the 16S rRNA gene. DNA fragment of phytoplasma with the size of 1.25 kbp was successfully amplified using primer pairs P1/P7, followed by primer pairs R16F2n/R16R2. Sequence analysis of the amplified fragments showed that phytoplasma associated with coconut wilt disease in Derawan island belongs to 16SrII (witches broom phytoplasma) and 16SrXI (ca. Phytoplasma oryzae) groups.

Genetic structure analysis of several peroral infectivity factor gene in Spodoptera litura Nucleopolyhedrovirus Christian, Michael; Kusumah, Yayi Munara; Mutaqin, Kikin Hamzah
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.125128-140

Spodoptera litura Nucleopolyhedrovirus (SpltNPV) is an entomopathogenic virus from the Baculoviridae family, currently under development as a biological control agent for cutworm Spodoptera litura. The NPV’s ability to infect its pest host can be determined by expression of pif protein complex by pif gene. The research aims to acquire genetic character information of the pif gene of SpltNPV from Bogor. Amplification of the NPV gene was carried out using the specific primers to amplifies two types of pif gene. The PCR products were sequenced then the DNA sequences were analyzed with the BioEdit and BLAST programs. The PCR amplification results showed that the size of the sample DNA fragment was 900 bp, 1300 bp and 710 bp. Based on the sequence analysis results, SpltNPV isolates from Bogor are closely related to SpltNPV and SpliNPV isolates from China. The highest nucleotide homology values of Pif-1, Pif-2, and Pif-3 gene were 99.56%, 99.37% and 100%, respectively. Based on the results of phylogenetic analysis, HearNPV isolates from Bogor belong to the same group as the NPVs that infect the species Spodoptera litura and closely related to NPV that infect Spodoptera littoralis. The amino acid sequence analysis showed the number of mutated sites in pif-1, pif-2, and pif-3 in this study are 1, 3, and 0, respectively, which indicates that protein mutation in SpltNPV Bogor did not significantly alter the viral infection process.

Teori Pemikiran Ekonomi Mikro Islam Perspektif Abu Ubaid dan Imam Al-Syaibani Mutaqin, Kikin; Sulaeman, Sulaeman; Janwari, Yadi; Jubaedah, Dedah
JURNAL ECONOMINA Vol. 3 No. 6 (2024): JURNAL ECONOMINA, Juni 2024
Publisher : LPPM Sekolah Tinggi Ilmu Ekonomi 45 Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/economina.v3i6.1350

Microeconomics is an economic science study that reviews individual behavior in conducting economic transactions. In the Islamic concept, microeconomics is characterized by the existence of norms that are applied within the limits of the Qur'an and Sunnah. The scholars of the classical period discussed many of these microeconomic issues, including Imam Abu Ubaid in his book al-Amwal and Imam al-Syaibani in his book al-kasb. This article explores the perspectives of these two influential men when examining microeconomic research through the use of descriptive qualitative methods and a review of relevant book and journal references. A Muslim scholar who specializes in Islamic economics is Imam Abu Ubaid whose ideas are contained in Kitab al-Amwal, one of the most influential scholarly references, containing many principles and concepts of public financial management. In addition to him, Iman As-Syaibani with his book Al-Kasb provides a view that work is human productivity that can make a person feel part of the world and has the value of worship for the pleasure of Allah SWT.

Pengembangan Sistem Pakar Identifikasi Awal Penyakit Kedelai Dengan Pendekatan Naïve Bayes Berbasis Android Astuti, Indah Puji; Hermadi, Irman; Buono, Agus; Mutaqin, Kikin H.
Jurnal Pustakawan Indonesia Vol. 14 No. 2 (2015): Jurnal Pustakawan Indonesia
Publisher : Perpustakaan IPB

Pengidentifikasian penyakit kedelai secara dini menjadi salah satu cara untuk meningkatan angka produktifitas kedelai. Jumlah pakar penyakit kedelai yang masih relatif sedikit apalagi di daerah pedesaan membuat ketergantungan atas keberadaan seorang pakar penyakit kedelai sangatlah tinggi terutama bagi para pemula di bidang pertanian. Suatu sistem pakar menjadi salah satu solusi yang dapat dijadikan sarana untuk berkonsultasi tentang penyakit kedelai layaknya seorang pakar. Sistem yang diimplementasikan dalam basis Android akan lebih mudah digunakan di manapun dan kapanpun tanpa harus bertemu dengan pakar karena kesempatan dan waktu pakar yang tidak mudah untuk ditemui setiap saat. Tujuan penelitian ini adalah untuk mengembangkan sistem pakar identifikasi awal penyakit kedelai dengan mengadopsi metode Expert System Development Life Cycle (ESDLC) untuk tahapan pengembangan sistem dan pendekatan Naïve Bayes sebagai metode inferensinya. Hasil penelitian ini berupa prototype sistem pakar XSIDS yang terdiri dari enam modul utama yaitu modul pengetahuan tentang kedelai, kebijakan pemerintah, konsultasi, tentang kami, tentang XSIDS dan note.Kata Kunci : Android, ESDLC, Naïve Bayes, Sistem Pakar, Penyakit Tanaman Kedelai

Molecular Characterization of Begomovirus Infecting Yard Long Bean (Vigna unguiculata subsp. sesquipedalis L.) in Java, Indonesia Nurulita, Sari; Hidayat, Sri Hendrastuti; Mutaqin, Kikin Hamzah; Thomas, John
BIOTROPIA Vol. 22 No. 1 (2015): BIOTROPIA Vol. 22 No. 1 June 2015
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2015.22.1.401

Begomovirus has been identified as one of the causal agents associated with yellow mosaic disease in yardlong bean (Vigna unguiculata subsp. sesquipedalis (L.)) in Java. Previous studies reported that Begomovirus has infected several Leguminosae species in South Asia, and several Begomoviruses have also been reported to infect important crops in Indonesia based on nucleotide sequence characterization. This study was conducted to identify and characterize Begomovirus isolated from yardlong bean samples in Java based on specific genomic features of the Common Region. Samples were collected from yardlong bean fields in Central Java (Tegal, Magelang, and Klaten), Yogyakarta (Sleman), and West Java (Bogor and Subang); viral detection was performed using I-ELISA, PCR, and sequencing; and molecular characterization was conducted using BioEdit v.7.0.5 and MEGA 6.06 software. Yellow mosaic disease was observed in nearly all surveyed fields, and infection of Potyvirus and Begomovirus was detected either as single or mixed infection. PCR results confirmed Begomovirus infection in samples from Tegal, Klaten, Magelang, Subang, and Bogor. Sequence analysis revealed that the virus infecting yardlong bean was Mungbean Yellow Mosaic India Virus (MYMIV) and shared close genetic similarity with isolates from Bangladesh, India, Pakistan, and Nepal. Further analysis revealed conserved regions surrounding the Common Region, including the “TATA box” sequence, hairpin-loop structure, repetitive sequences, and the conserved nonanucleotide TAATATTAC. This study provides the first report of MYMIV infection in Indonesia.

Co-Authors . Giyanto Abdjad Asih Nawangsih Abdul Munif Ade Syahputra Af’idzatuttama Agus Buono Agus Eko Prasetyo Agus Purwito Ali Husni ALI NURMANSYAH Ana Feronika Cindra Irawati Ani Widarti Anna Feronika Cindra Irawati Ariny Prasetya Asmar Hasan Astuti, Indah Puji Asysyuura, Asysyuura Auliya Selamet Bonny Poernomo Wahyu Soekarno Budi Tjahjono Budiyarto . Christian, Michael Dede Maryana Diana Putri Dono Wahyuno Efi Toding Tondok Eny Widajati Fitri Kemala Sandra Fitrianingrum Kurniawati, Fitrianingrum Fransina Latumahina Gede Suastika Giyanto Giyanto . Giyanto Giyanto Giyanto Giyanto Giyanto Giyanto Giyanto, Giyanto Hagia Sophia Khairani Hamidi, Ilmi Hidayat, Sri Indah Puji Astuti Indriati Husain Irman Hermadi Irsan Nuhantoro Isti Wulandari John Thomas, John Jubaedah, Dedah Kresnamurti Kurniasih Kristi, Agusti Kusumah, Yayi Munara Laksono Trisnantoro Leiwakabessy, Christoffol Maggy Tenawidjaja Suhartono Maggy Tenawidjaja Suhartono MEITY SURADJI SINAGA Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Miftakhurohmah Muhammad Firdaus Oktafiyanto Muhammad Rizal nFN Sulastri nFN Widodo Nurfadillah PURNAMA HIDAYAT Rita Kurnia Apindiati Rizky G S Purnama RUSMILAH SUSENO Sahetapy, Betty Sari Nurulita Siska Irhamnawati Pulogu Slamet Susanto Sofranita Syifa Fitriyati SRI HENDRASTUTI HIDAYAT Sri Kurniawati Sudir Sudir, Sudir Sulaeman Sulaeman Sulastri Sulastri Supramana Supriadi Suryo Wiyono Syafrida Manuwoto SYAFRIDA MANUWOTO Tatit Sastrini Tita Widjayanti TRI ASMIRA DAMAYANTI Trikoesoemaningtyas Wahyudin, Denih Wartono, Wartono Widi Amaria Widodo Widodo Widodo Widodo Yadi Janwari Yayu Siti Nurhasanah Yudi Sastro Yudi Sastro Yuliana Susanti Yuliani, Fitria

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