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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Pustakawan Indonesia Agrikultura BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Hortikultura JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Informatika Pertanian Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Pustakawan Indonesia JURNAL ECONOMINA

Kikin H Mutaqin

Institut Pertanian Bogor

Author-ID : 298800

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Immunology & microbiology Library & Information Science Medicine & Pharmacology Social Sciences Veterinary

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Lama Penyimpanan, Karakterisasi Fisiologi, dan Viabilitas Bakteri Endofit Bacillus sp. dalam Formula Tepung Diana putri; Abdul Munif; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 12 No 1 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Endophytic bacteria can be formulated to retain its ability as disease control agents. Three of endophytic bacteria which had the capability to suppress infection of Meloidogyne sp, and to enhance pepper growth were gained from the previous study. This research was aimed to evaluate the influence of storage time on the viability of endophytic bacteria, Bacillus sp. AA2, Bacillus sp. MER and MSJ, and to study its physiological charaterization during storage. The formulation evaluated in this study was : formulation 1 (50 g talc, 1 g pepton, 0.5 g CMC, and brown sugar 1.5 g), formulation 2 (50 g talc, 1 g pepton, 0.5 g CMC, and 1.5 g white sugar), formulation 3 (50 g talc, 1 g pepton, 0.5 g CMC, 1 g yeast extract, and 1.5 gwhite sugar) and formulation 4 (50 g talc, 1 g pepton, 0.5 g CMC, 1 g yeast extract, 3 mL molasses, 1 gbentonite, 0.75 g calcium carbonate, and 1 g dextrose). The results of the bacterial characterization showed that Bacillus sp AA2 and Bacillus sp MER belongs to Gram positive, produced lipase and protease enzyme, as well as IAA hormone. N2 fixation is only existed in Bacillussp. AA2 and MSJ isolate. The highest viability was shown on MSJ isolate with 2.5×106 cfu mL-1. in the fourth formulation, whereas Bacillus sp. AA2 and Bacillus sp. MER viability was 1.9×106 cfu mL-1. and 1.2×106 cfu mL-1. , respectively.

Komparasi Metode Isolasi DNA Patogen Antraknosa dan Bulai untuk Deteksi PCR Ade Syahputra; Kikin Hamzah Mutaqin; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol 12 No 4 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Polymerase chain reaction (PCR) is an important tool for detection, identification and monitoring of quarantine pests in Indonesia. DNA isolation method from target organism is an important step to provide adequate DNA template for performing PCR. Objective of the research was to compare conventional, commercial kit, FTA-card and its modification methods of DNA isolation to be used in PCR detection for Colletotrichum acutatum and Peronosclerospora sorghi from chili and maize, respectively. DNA obtained from various isolation methods were measured using UV-vis nanodrop-spectrophotometry. DNA amplification was performed using DNA concentration of 15 ng µL-1 from each isolation method with gradual primer concentrations of 0.4; 0.6; 0.8; and 1.0 mM. The highest concentration of DNA was achieved with conventional methods for C. acutatum from pure culture and P. sorghi from maize leaf. Best DNA purity was obtained from isolation method using commercial kit for C. acutatum from infected fruit (1.94) and from conventional method for C. acutatum from pure culture (1.91). The highest total yield of isolated DNA was achieved by modified FTA-card for C. acutatum from pure culture. In general DNA amplification using various primer concentration gave positive results although DNA bands intensity was varied from faint to very bright. Furthermore PCR optimization using the best primer concentration from previous reaction showed that all DNA templates resulted in thick and bright DNA bands.

Cendawan Endofit yang Potensial Meningkatkan Ketahanan Cabai Merah terhadap Penyakit Layu Bakteri Ana Feronika Cindra Irawati; Yudi Sastro; Sulastri Sulastri; Maggy Tenawidjaja Suhartono; Kikin Hamzah Mutaqin; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 12 No 4 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Ralstonia solanacearum has been known to cause bacterial wilt disease on chili pepper. Despite many reports on the potential use of endophytic fungi to induce plant resistance, its utilization to suppress bacterial wilt disease of chili has not been widely reported. The aims of this research was to screen potential endophytic fungi that may increase chili resistance against bacterial wilt disease. Selection of endophytic fungi was done using in vivo and antibiosis test. Strains of fungi were considered the most potent in suppressing the development of bacterial wilt in chilli were identified. Ten out of 62 isolates of endophytic fungi gave the highest suppression on chilli’s bacterial wilt disease. Most strains of endophytic fungi were able to suppress the development of bacterial wilt disease, but not always positively correlated to the vegetative and generative growth of chilli. Based on the level of disease intensity and the growth of plants were obtained three strains of endophytic fungi that considered potentially suppress the incidence of bacterial wilt disease. The three isolates was identified as Fusarium solani f.sp. phaseoli (AC-2.13 and AC-4.4) and Trichoderma asperellum (AC-3.18) using morphology and molecular characters. Although all three selected isolates were able to suppress bacterial wilt disease in this study, but application of F. solani f.sp. phaseoli should be considered in practical use since it is generally known as the causal agent of root rot disease of beans

Deteksi dan Evaluasi Keragaman Genetika Candidatus Liberibacter asiaticus sebagai Penyebab Penyakit Huanglongbing di Indonesia Berdasarkan Gen β-operon Muhammad Rizal; Kikin Hamzah Mutaqin; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 12 No 5 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Huanglongbing also known in Indonesia as citrus vein phloem degeneration (CVPD) is a devastating disease in citrus plantation worldwide, especially in Asia, Africa, and America. In Asian countries including Indonesia, Candidatus Liberibacter asiaticus (CLas) has been confirmed as the causal agent of huanglongbing disease on citrus. Distribution of CLas in Indonesia has been reported in West Borneo, East Nusa Tenggara, Bali, Yogyakarta, Central Java and East Java. The purpose of this study was to detect CLas in several Indonesia’s citrus plantations that has not and has been reported previously and to study its genetic diversity and their relationship. DNA of plant samples, i.e. citrus leaves, was extracted using CTAB method and CLas was amplified using PCR with the A2/J5 primer pair. DNA amplification results showed that infection of CLas was positively detected from samples from Bogor and Cibodas (West Java), Tuban and Jember (East Java), as well as Katung, Bayung Gede, Kerta, and Pancasari (Bali). Alignment of nucleotide sequences from positive samples showed that their ribosomal protein β-operon has high similiarity to that of CLas OK901 originated from Okinawa (Japan). Isolates of CLas originated from Bogor, Cibodas, Tuban, Jember, and Katung have been known to be identical to other CLas of Indonesian origins reported earlier. Isolates of CLas originated from Bayung Gede, Pancasari, and Kerta have single nucleotide polymorphisms at 6 points of bases of the 539 total bases compared in their conservative regions, although only 3 of the 6 bases could affect their amino acid sequences.

Penggunaan Pelacak DNA untuk Deteksi Papaya ringspot virus dengan Metode Hibridisasi Asam Nukleat Irsan Nuhantoro; Sri Hendrastuti Hidayat; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol. 14 No. 3 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Use of DNA Probe for Detection of Papaya ringspot virus Using Nucleic Acid Hybridization MethodPapaya ringspot caused by Papaya ringspot virus (PRSV) is one of the most destructive diseases of papaya. The disease had not been found in Indonesia, until disease outbreak in Nangroe Aceh Darussalam was reported in 2012. Since then, the disease spread rapidly in most papaya growing areas in Sumatera, Java and Bali. Papaya ringspot virus (PRSV) is generally detected using serological or polymerase chain reaction methods. Improvement in detection method is necessary to facilitate a more reliable tool for controlling the spread of PRSV. The aim of the research was to construct DNA probe for development of detection method based on nucleic acid hybridization. Molecular characterization based on HCPro gene sequence indicated high homology (97.88 to 99.05%) among PRSV isolates from Boyolali (Central Java), Medan (North Sumatera), Sleman (Yogyakarta) and Tabanan (Bali). Two DNA clones of HCPro gene were selected for probe construction and the probes were then labeled using PCR DIG-dioxigenin. Optimization of nucleic acid dot blot hybridization method to achieve strongest positive reaction was developed, i.e. using stringency washes at 1×SSC, 0.1% SDS, incubation at 60 oC for 15’. The DNA probe for PRSV has a high specificity and sensitifity; it could detect PRSV at the lowest concentration of nucleic acid (0.062 µg µL-1).

Identifikasi Molekuler Fitoplasma yang Berasosiasi dengan Tanaman Kaktus Hias Opuntia sp. Ariny Prasetya; Kikin Hamzah Mutaqin; Meity Suradji Sinaga; Giyanto Giyanto
Jurnal Fitopatologi Indonesia Vol 13 No 4 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Cactus species (Opuntia sp.) is a popular ornamental succulent plant. Some ornamental cactus species in Indonesia showing proliferation and green mosaic pattern symptoms have been reported to be associated with phytoplasma infection. However, further molecular identification for accurate classification of the causal phytoplasma has not been done. This study aimed to diagnose phytoplasma associated with Opuntia sp. based on molecular methods involving PCR standard combined with nested-PCR, cloning and DNA sequencing. Standard PCR was carried out using P1/P7 primers followed by nested-PCR using R16F2n/R16R2 or fU5/rU3 primer pairs which amplify the 16S rRNA gene targets of 1.2 kb and 880 bp, respectively. Amplified fragment of nested-PCR using R16F2n/R16R2 primers was chosen to be cloned and sequenced for further identification and classification of phytoplasma. BLASTn analysis showed that the phytoplasma from Opuntia sp. was closely related to 16SrII group. Phylogenetic analysis and in silico RFLP indicated that phytoplasma strain infecting Opuntia sp. was a member of subgroup 16SrII-C (cactus witches’ broom phytoplasma). This is a newly report of cactus witches’ broom phytoplasma on Opuntia sp. in Indonesia.

Keefektifan Asap Cair dan Elektroterapi untuk Mengeliminasi Infeksi Xanthomonas oryzae pv. oryzae pada Benih Padi Rizky G S Purnama; Kikin Hamzah Mutaqin; Efi Toding Tondok
Jurnal Fitopatologi Indonesia Vol. 14 No. 2 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

The Effectiveness of Liquid Smoke and Electrotherapy to Eliminate Infection of Xanthomonas oryzae pv. oryzae in Rice SeedBacterial leaf blight caused by Xanthomonas oryzae pv. oryzae is a seed-borne disease that can reduce the productivity of rice. Alternative treatments that have not been widely developed is application of liquid smoke and electrotherapy. The aim of this research was to study the effectiveness of liquid smoke and electrotherapy and their combinations to eliminate X. oryzae pv. oryzae without damaging seed quality. The study consisted of three experimental stages: (1) Liquid smoke treatment on the viability of the bacteria and rice seeds; (2) Electrotherapy treatment on the viability of bacteria and rice seeds; (3) Combination of liquid smoke and electrotherapy treatment in bacteria-infected rice seed. The results showed that liquid smoke concentration (5% for 30 minutes) was able to reduce 83.33% population of X. oryzae pv.oryzae on rice seeds with 94.33% seed vigor, 98.00% germination, and time required to achieve 50% of total seed emergence (T50) of 3.13 days. Electrotherapy treatment (400 mA for 20 minutes) was not only eliminated X. oryzae pv. oryzae on rice seeds up to 100% but also maintained seed vigor 81.67%, germination 89.33%, and T50 3.47 days. Electrotherapy treatment (400 mA for 20 minutes) followed by immersion of infected rice seeds in liquid smoke concentration (1% for 30 minutes) could reduce 94.59% bacteria population while maintaining 86.00% seed germination. Treatment of electrotherapy (400 mA for 20 minutes) followed by immersion seeds into liquid smoke concentration (5% for 30 minutes) could reduce bacterial population up to 100%, but caused phytotoxicity effect to the seedling.

Asam Salisilat sebagai Penginduksi Ketahanan Tanaman Padi terhadap Penyakit Hawar Daun Bakteri Christoffol Leiwakabessy; Meity Suradji Sinaga; Kikin Hamzah Mutaqin; Trikoesoemaningtyas Trikoesoemaningtyas; Giyanto Giyanto
Jurnal Fitopatologi Indonesia Vol. 13 No. 6 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

The use of salicylic acid as resistance inducers agents in several plants species was well known. Salicylic acid has been believed to play an important role in inducing resistance against some pathogen. This research was aimed to study the role and effectiveness of salicylic acid as inducers for the resistance of some rice variety against bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae. The experiment was arranged in completely randomized factorial design with three factors i.e. rice variety, salicylic acid, and pathotypes, each treatment was replicated three times. The results showed that salicylic acid could prolong the latent period of X. oryzae pv. oryzae pathotypes IV and VIII in paddy rice varieties Conde and Ciherang, induce the resistance of rice varieties as well as reducing the rate of infection of the two mentioned pathotypes. Salicylic acid was also able to induce the resistance of Conde and Ciherang varieties in order to suppress area under disease progress curve (AUDPC) of X. oryzae pv. oryzae pathotypes IV and VIII.

Aktivitas Antagonis Bakteri Endofit Asal Mangrove terhadap Ralstonia solanacearum dan Meloidogyne spp. Muhammad Firdaus Oktafiyanto; Abdul Munif; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 14 No 1 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

The main diseases of tomato caused by wilt bacteria (Ralstonia solanacearum) and root knot nematodes (Meloidogyne spp.) may cause significant yield losses and need to be managed. Recently, biocontrol approach especially using endophytic bacteria has been developed to control plant pathogens. This research was aimed to obtain endophytic bacteria from 2 species of mangrove, which effectively controlled root knot nematode and wilt bacteria. A total of 843 endophytic bacteria were successfully isolated from 2 species of mangroves. The bacteria isolates were further subjected for biosafety assay. The results of the test showed that 403 endophytic bacteria gave negative reaction in hypersensitive and hemolytic tests. Furthermore, 19 isolates effectively suppressed the growth R solanacearum and killed Meloidogyne in in vitro test. Physiological test showed that 14 and 11 isolates of the bacteria were able to produce protease and chitinase, respectively. This research provides a new information that endophytic bacteria from mangrove has a potency as a biocontrol agents.

Morfologi dan Morfometri Nematoda Sista Kentang (Globodera spp.) Asal Dataran Tinggi Dieng, Jawa Tengah Auliya Selamet; Supramana Supramana; Meity Suradji Sinaga; Ali Nurmansyah; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 15 No 2 (2019)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

The Morphology and Morphometry of Potato Cyst Nematodes (Globodera spp.) from Dataran Tinggi Dieng, Central JavaIn Indonesia, the potato cyst nematode (PCN) was first reported in 2003, at potato plantation in Batu, East Java. Golden cyst (Globodera rostochiensis) was detected in Bandung, West Java; Banjarnegara, Central Java; Batu, East Java; and Gowa, South Sulawesi, whereas the pale cyst (G. pallida) has been reported at limited distribution in Dataran Tinggi Dieng (Central Java). The aim of this research was to identify the Globodera species of Dataran Tinggi Dieng based on morphological and morphometrical methods. Soil samples were collected from Dieng plateau at 30 selected potato plantations ranging from 1 100 – 2 100 m above sea level. The identification of PCN was done by observing the morphological and morphometrical key characters of cysts and second juveniles (J2). Two species of Globodera, that are G. rostochiensis and G. pallida, were identified from soil samples. The key characters of G. pallida including distinct forward projection of J2 stylet knob, granek’s ratio value less than 3, and number of cuticular ridges between vulval basin and anus are less than 12 were identified. There were 25 specimens that having granek’s ratio less than 3, and 10 specimens having cuticular ridges number less than 12. G. rostochiensis which has the shape of a stylet knob protrudes posteriorly and the value of the granek’s ratio greater than 3.5 was identified in 30 specimens.

Co-Authors . Giyanto Abdjad Asih Nawangsih Abdul Munif Ade Syahputra Af’idzatuttama Agus Buono Agus Eko Prasetyo Agus Purwito Ali Husni ALI NURMANSYAH Ana Feronika Cindra Irawati Ani Widarti Anna Feronika Cindra Irawati Ariny Prasetya Asmar Hasan Astuti, Indah Puji Asysyuura, Asysyuura Auliya Selamet Bonny Poernomo Wahyu Soekarno Budi Tjahjono BUDI TJAHJONO Budiyarto . Christian, Michael Dede Maryana Diana Putri Dono Wahyuno Efi Toding Tondok Eny Widajati Fitri Kemala Sandra Fitrianingrum Kurniawati, Fitrianingrum Fransina Latumahina Gede Suastika Giyanto Giyanto . Giyanto Giyanto Giyanto Giyanto Giyanto Giyanto Giyanto, Giyanto Hagia Sophia Khairani Hamidi, Ilmi Hidayat, Sri Indah Puji Astuti Indah Puji Astuti Indriati Husain Irman Hermadi Irsan Nuhantoro Isti Wulandari John Thomas Jubaedah, Dedah Kresnamurti Kurniasih Kristi, Agusti Kusumah, Yayi Munara Laksono Trisnantoro Leiwakabessy, Christoffol Maggy Tenawidjaja Suhartono Maggy Tenawidjaja Suhartono MEITY SURADJI SINAGA Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Miftakhurohmah Muhammad Firdaus Oktafiyanto Muhammad Rizal nFN Sulastri nFN Widodo Nurfadillah PURNAMA HIDAYAT Rita Kurnia Apindiati Rizky G S Purnama RUSMILAH SUSENO Sahetapy, Betty Sari Nurulita Siska Irhamnawati Pulogu Slamet Susanto Sofranita Syifa Fitriyati SRI HENDRASTUTI HIDAYAT Sri Kurniawati Sudir Sudir, Sudir Sulaeman Sulaeman Sulastri Sulastri Supramana Supriadi Suryo Wiyono Syafrida Manuwoto SYAFRIDA MANUWOTO Tatit Sastrini Tita Widjayanti TRI ASMIRA DAMAYANTI Trikoesoemaningtyas Wahyudin, Denih Wartono, Wartono Widi Amaria Widodo Widodo Widodo Widodo Yadi Janwari Yayu Siti Nurhasanah Yudi Sastro Yudi Sastro Yuliana Susanti Yuliani, Fitria

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