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All Journal HAYATI Journal of Biosciences JURNAL ILMIAH PLATAX Jurnal Bios Logos JSME MIPA UNIMA Abdimas Ngayah: Majalah Aplikasi IPTEKS Animal Production : Indonesian Journal of Animal Production Jurnal Biologi Tropis JURNAL PENGABDI Dinamisia: Jurnal Pengabdian Kepada Masyarakat MATAPPA: Jurnal Pengabdian Kepada Masyarakat JURNAL PENDIDIKAN TAMBUSAI Jurnal Pembelajaran dan Biologi Nukleus SOSCIED Jurnal Aplikasi dan Inovasi Iptek (JASINTEK) Bakti Cendana: Jurnal Pengabdian Masyarakat Jurnal Dedikasi Bioscientist : Jurnal Ilmiah Biologi Abdimas : Jurnal Pengabdian Kepada Masyarakat Seminar Nasional Pengabdian Kepada Masyarakat Indonesian Biodiversity Journal Jurnal Pengabdian Masyarakat dan Riset Pendidikan Indonesian Biodiversity Journal Servitium Smart Journal Advances in Tropical Biodiversity and Environmental Sciences
Revolson Mege
Biology Department, Faculty Of Mathematics And Natural Sciences, Manado State University
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : JURNAL ILMIAH PLATAX

 1 
A New and Practical Method for Measuring Sponge Spicules Rieuwpassa, Frets J.; Tomasoa, Aprelia M.; Palawe, Jaka F. P.; Rieuwpassa, Fredrik; Mege, Revolson Alexius; Balansa, Walter
Jurnal Ilmiah Platax Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.47882

Abstract

Binocular light microscopy (BLM) is an excellent match for a scanning electron microscope (SEM) and a trinocular light microscope equipped with a micrometer (TLM). The practicality, user-friendliness, and short-time analysis of BLM make this method a good choice for spicule analysis. However, its effectiveness and accuracy are yet to be confirmed. This study aimed to validate the effectiveness of BLM by comparing its usefulness to both TLM and the gold standard methods. BLM was first subjected to measuring megascleres and microscleres of 2 sponges. Then, by using the If function built-in Excell and t-test in SPSS 16.0, the compatibility of BLM was evaluated against SEM by measuring the length of spicules from 4 Sangihe sponges and their counterpart species from different locations. Furthermore, the t-test analysis was used to validate the compatibility and effectiveness of our method to the TLM by measuring the spicules of four sponges. Both the F-function and the t-test analysis proved BLM was compatible with SEM with both measurements showing a perfect match for megascleres typed spicules of 4 compared sponges. This new technique also showed a perfect match with SEM (p = 0.367, t-test) and with TLM (p = 0.963, t-test). Keywords: Spicules, sponges, SEM, Wallacea, biomaterial, sponge taxonomy
Antifungal Potential Of The Sponge Styllisa Flabelliformis Against The Pathogenic And Resistant Aspergillus Fungi Tomasoa, Aprelia Martina; Rieuwpassa, Frets Jonas; Azhari, Deidy; Ijong, Frans Gruber; Mege, Revolson Alexius; Tanod, Wendy Alexander; Balansa, Walter
Jurnal Ilmiah Platax Vol. 11 No. 1 (2023): ISSUE JANUARY-JUNE 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i1.48603

Abstract

Together with bacterial and viral infection, fungal infection represents the world's top ten killer diseases, desperately requiring new antifungal drugs. This research aimed to evaluate the antifungal activity of the sponge Styllisa flabelliformis against the pathogenic fungi Aspergillus flavus and Aspergillus parasiticus by the standard agar diffusion technique. Three concentrations (1, 10, and 10 mg/mL) for the extract and 0.5 mg/mL for ketoconazole and fluconazole were prepared and evaluated in triplicate against the tested fungi. Whereas ketoconazole poorly inhibited A. parasiticus and fluconazole weakly inhibited A. flavus, the extract of S. flabelliformis exerted antifungal activity against A. parasiticus (6.8 ± 1.8; 8.3 ± 3.2; and 9.5 ± 2.1) mm and A. flavus (6.8 ± 1.1; 11.5 ± 1.4; and 14.3 ± 1.1) mm 1, 10 and 100 mg/mL respectively. PASS analysis showed jasplakinolide as a promising antifungal agent with potential activity (Pa) of 0.736. STITCH analysis further confirmed that jasplakinolide worked by inhibiting the expression of cytoskeleton genes that prevented fungi from synthesizing chitin and inhibiting the formation of the fungi’s cell walls and hyphae, different from the ergosterol synthesis inhibition in ketoconazole and fluconazole, implying the potential of jasplakinolide as an antifungal agent.
Co-Authors A. Nurfitriani Alberto Y T Allo Alfonds Andrew Maramis Alfrina Mewengkang Anita Costanci Christine Tengker Aprelia Martina Tomasoa, Aprelia Martina Arifin Arifin Armstrong F. Sompotan Arrijani - Aser Yalindua Azhari, Deidy Balansa, Hendra Balansa, Walter Banea, Agnes Buyang, Dalle Christny Ferdina Evie Rompas Danny Ch Posumah Dany Posumah Debby Jacqualine Jochebed Rayer Dino Rahardiyan Diswal Takasaheng Edwin Mangore Emma M Moko Emma Mauren Moko Fadly Steven Jefry Rumondor Fanny Nella Nanlohy Femmy Nurul Akbar Femmy Roosje Kawuwung Ferencia Esananda Rattu Fitriani, A. Nur Frans Gruber Ijong Frets Jonas Rieuwpassa Friska Mery Montolalu Grace Jenny Soputan Grichelle Tampi Gua, Siskarli Helen Joan Lawalata Herry Maurits Sumampouw Ika Okhtora Angelia IRIANI SETYAWATI Iriani Setyawati Iriani Setyawati Jabes Wolter Kanter Janne Ticoh Jantje Ngangi Jantje Pelealu Jaqueline E.M Tangkau Jeane Mantiri Jeane Tuilan Jennifer Pangulimang Johanna Zusye Wantania Jonathan Polandos julduz ruland paus Julduz Ruland Paus Juninho Siwu Karuntu, Rilya Patricia Esther Kezia Andries Laksono Trisnantoro Lawalata, Helen Lensehe, Febby Anggini Livana Dethris Rawung Maharani Angelika Elshadai Rori Maidangkay, Vonny Maiko Polandos Manampiring, Noni Manampiring, Nonny Mansyur, Djailani Maria Helena Mariane Pangandaheng Mario Tulenan Parinsi Max Tulung Megawati Meylin Weken Megawati Weken Meity Nelltje Tanor Mellinia Tawas Mervin Tooy, Stephanie Meyer Worang Matey Misgter Gidion Maru Mokosuli Yermia Samuel Mukuan, Randi Mundaeng, Camel Gantonio N Kusumorini, N NASTITI KUSUMORINI Nerni Potolangi Nonny Manampiring Nova L.I.M Ogi Palawe, Jaka F. P. Patricia S. M Mantiri Paulus Boli Pinaria, Yeanly Wuena Pomalingo, Moh Fikri Pratasik, Stralen Pratasik, Veronika Pricilia Joice Pesak Purba, Gandi YS Quido Conferti Kainde Rahel Kimbal Rampengan, Mercy Maggy Franky Recky H.E. Sendouw Rieuwpassa, Fredrik Rieuwpassa, Frets J. Rolef Rumondor Rolef Rumondor Rompas, Christny Ferdina Evie Roring, Verawaty Ida Yani Rymond Jusuf Rumampuk Sakul, Ernest Hanny Sambeka, Delvino Sasauw, Dien A. Semuel Lasut SH Nasution, SH Sigit Prafiadi Sintia Djafar Sukmarayu Gedoan Sukmarayu P. Gedoan Syahira, Syahira SYAHRUN HAMDANI NASUTION Toheni, Fredrik Tomasoa, Aprelia M. Umboh, Aprillia V. Utari Satiman Vanessa Dagasina Verawati I Y Roring Verawati Ida Yani Roring Veronika Waroka Verra E. J. Mawitjere W Manalu Walter Balansa Wasmen Manalu Wendy Alexander Tanod Wisda Nurjannah Abdullah Wiska Baharuddin Cahdeso Wulandari Wulandari