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All Journal Buletin Peternakan JURNAL KIMIA SAINS DAN APLIKASI Jurnal Pendidikan Biologi Undiksha Jurnal Biologi dan Pembelajarannya (JBP) JURNAL PENGABDIAN KEPADA MASYARAKAT Pro-Life BERITA BIOLOGI Biodidaktika : Jurnal Biologi dan Pembelajarannya Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat BIOEDUSCIENCE Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA JMM (Jurnal Masyarakat Mandiri) MATAPPA: Jurnal Pengabdian Kepada Masyarakat Jurnal Saintek Lahan Kering JPBIO (Jurnal Pendidikan Biologi) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Community Development Journal: Jurnal Pengabdian Masyarakat BERNAS: Jurnal Pengabdian Kepada Masyarakat Bakti Cendana: Jurnal Pengabdian Masyarakat Journal of Tropical Animal Science and Technology Jurnal Pengabdian UNDIKMA Jurnal Biosense Bioscientist : Jurnal Ilmiah Biologi Prosiding Konferensi Nasional PKM-CSR Society: Jurnal Pengabdian Masyarakat Dharma Pengabdian Perguruan Tinggi (DEPATI) JURNAL BIOLOGI PAPUA Jurnal Abdimas Maduma BIO-CONS: Jurnal Biologi dan Konservasi Quagga: Jurnal Pendidikan dan Biologi Journal Science of Biodiversity Journal Science of Biodiversity BIOGENIC: Jurnal Ilmiah Biologi Journal of Chemical Science and Application Berita Biologi
Lukas Pardosi
Prodi Biologi, Universitas Timor, Indonesia
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Journal : Journal of Tropical Animal Science and Technology

 1 
Isolation and Identification of Bacteria from Cattle Rumen Fluid and Their Application on In Vitro Digestibility of Rice Straw Bira, Gerson Frans; Sio, Stefanus; Batu, Matius S; Pardosi, Lukas; Kia, Kristoforus W; kolo, Maria Magdalena; Nubatonis, Alfred
Journal of Tropical Animal Science and Technology Vol. 7 No. 3 (2025): Journal of Tropical Animal Science and Technology
Publisher : Animal Husbandry Study Program, Faculty of Agriculture, Timor University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aimed to isolate and identify bacteria from the rumen fluid of Bali cattle and to evaluate their effect as microbial inoculants on the in vitro digestibility of rice straw. The experiment was conducted using a Completely Randomized Design (CRD) consisting of four treatments and four replications. The treatments included rice straw silage with the addition of 0%, 5%, 10%, and 15% of local microorganisms (MOL) derived from cattle rumen fluid. The observed variables included the isolation and identification of bacteria from the rumen fluid, and the assessment of in vitro digestibility, including Dry Matter Digestibility (DMD), Organic Matter Digestibility (OMD), Volatile Fatty Acid (VFA) concentration, and ammonia (NH₃) production. The results showed that the addition of rumen fluid MOL significantly (P<0.05) improved the digestibility parameters. The highest DMD (52.44%) and OMD (47.11%) were obtained from the treatment with 15% MOL. VFA and NH₃ concentrations also increased, indicating enhanced microbial activity and fermentation efficiency. The study concludes that MOL derived from cattle rumen fluid, particularly those containing Bacillus sp., is effective in improving the nutritional quality and digestibility of rice straw
Isolation and Identification of Bacteria from Cattle Rumen Fluid and Their Application on In Vitro Digestibility of Rice Straw Bira, Gerson Frans; Sio, Stefanus; Batu, Matius S; Pardosi, Lukas; Kia, Kristoforus W; kolo, Maria Magdalena; Nubatonis, Alfred
Journal of Tropical Animal Science and Technology Vol. 7 No. 3 (2025): Journal of Tropical Animal Science and Technology
Publisher : Animal Husbandry Study Program, Faculty of Agriculture, Timor University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v7i3.10038

Abstract

This study aimed to isolate and identify bacteria from the rumen fluid of Bali cattle and to evaluate their effect as microbial inoculants on the in vitro digestibility of rice straw. The experiment was conducted using a Completely Randomized Design (CRD) consisting of four treatments and four replications. The treatments included rice straw silage with the addition of 0%, 5%, 10%, and 15% of local microorganisms (MOL) derived from cattle rumen fluid. The observed variables included the isolation and identification of bacteria from the rumen fluid, and the assessment of in vitro digestibility, including Dry Matter Digestibility (DMD), Organic Matter Digestibility (OMD), Volatile Fatty Acid (VFA) concentration, and ammonia (NH₃) production. The results showed that the addition of rumen fluid MOL significantly (P<0.05) improved the digestibility parameters. The highest DMD (52.44%) and OMD (47.11%) were obtained from the treatment with 15% MOL. VFA and NH₃ concentrations also increased, indicating enhanced microbial activity and fermentation efficiency. The study concludes that MOL derived from cattle rumen fluid, particularly those containing Bacillus sp., is effective in improving the nutritional quality and digestibility of rice straw
Giblet Organs and Digestive Performance of Native Chickens in the Pullet Phase Fed Diets Supplemented with Propionic Acid Tae, Agustina Viktoria; Naisali, Yuliana; Lisnahan, Charles V.; Pardosi, Lukas
Journal of Tropical Animal Science and Technology Vol. 8 No. 1 (2026): Journal of Tropical Animal Science and Technology
Publisher : Animal Husbandry Study Program, Faculty of Agriculture, Timor University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v8i1.10601

Abstract

The purpose of this study was to examine the effect of propionic acid levels in feed on the performance of giblet organs and digestive tracts of pullet-phase Native chickens. A total of 80 Native chickens aged 12 weeks with an average weight of 400 g were used in this study. The research design applied was a Completely Randomized Design (CRD). Chickens were randomly divided into four treatments with four replications, where each replication consisted of four Native chickens. The treatments given were T0: control feed without propionic acid; T1: control feed + 0.25% propionic acid; T2: control feed + 0.50% propionic acid; T3: control feed + 0.75% propionic acid. Data analysis was conducted using ANOVA, followed by the Duncan Test to determine differences between treatments. The results showed that live weight, giblet weight, pancreas weight, intestine weight, intestine length, proventriculus weight, were highest in the T3 control feed + 0.75% propionic acid treatment, respectively, at 1622.36±38.24 g/head; 59.23±0.39 g/head; 3.54±0.04 g/head; 27.24±0.57 g/head; 27.24±0.57 g/head; 129.33±0.68 cm/head; 6.04±0.18 g/head. The highest giblet percentage and proventriculus percentage were in the T0 control feed treatment without propionic acid at 3.74±0.12%/head and 0.38±0.01%/head, respectively. The highest percentage of pancreas weight was in the T2 treatment of control feed + 0.50% propionic acid and the T3 treatment of control feed + 0.75% propionic acid, which was 0.22 ± 0.01%/head. The highest percentage of intestine weight was obtained in the T2 treatment (control feed + 0.50% propionic acid) with a value of 1.71 ± 0.03%/head. Analysis of variance showed that the treatment significantly affected the live weight, giblet organ weight, pancreas weight, percentage of pancreas weight, small intestine weight, intestine length, and proventriculus weight of pullet phase Native chickens (P <0.05). It can be concluded that the addition of 0.75% propionic acid to the control feed had a positive effect on the performance of the giblet organ and digestive tract of Native chickens.
Co-Authors Agustina Yovita Boluk Alfred Nubatonis Anastasia Buak Anggelina Mokos Anjelina M. da Cruz Arkianti, Maria Maxmilla Yoche Azor Yulianus Tefa Banusu, Maria Sarliana Batu, Matius S Benu, Didi Prasetyo Bira, Gerson Frans Bria, Deseriana Charles Venirius Lisnahan Dira Asri Pramita Eduardus Yosef Neonbeni Elmalinda Moensaku Emanuel M. Y. Hano’e Emanuel M. Y. Hano’e Emilia Juliyanti Bria Faesal, Andi Fallo, Gergonius Fitra Ramadhan Florian Mayesti Prima Remba Makin Fobia, Kaprisius G. Fransiskus Yulius Dhewa Kadju Hanas, Dicky Frengky Hano’e, Emanuel M. Y. Hermina Manlea Hermina Manlea I Gede Arya Wiguna Jefry Presson Jefry Presson jefry Presson Jendri Mamangkey Jonris Tampubolon Josua Sahala Jusanty Antoneta Pakh Kamaluddin Kamaluddin Kamaluddin Kamaluddin Kia, Kristoforus W Kia, Kristoforus Wilson Kia, Kristoforus. W. Kolo, Maria Magdalena Korbafo, Elisabeth Kristoforus Wilson Kia Lidya, Christie Liem, Noviana Animra Lisnahan, Charles V. Lusiana Yeni Akoit Mada, Grandianus Seda Manalu, Adelya I Mardit N Nalle Maria Ernalinda Seran Matius Stefanus Batu Meta Permata Putri Metriana Leon Moensaku, Elmalinda Nahak, Oktovianus Rafael Naimuni, Fransiskus Xaverius Naisali, Yuliana Natalia Desy Djata Ndua Natalia Hoar Neonbeni, Eduardus Yosef Nugraha K. F. Dethan Pakh, Jusanty Antoneta Pramita, Dira Asri Pramusita, Santa Maya Presson, Jefry Respati Tri Swasono Riska Septia Wahyuningtyas Risna Erni Yati Adu Sabirin Matsjeh Sefrinus Maria Dolfi Kolo Seuk, Mariana Septiani Siahaan, Desta Gloria Sine, Yuni Sio Stefanus, Sio Sipayung, Boanerges Putra SITUMORANG, KOMILIE Tae, Agustina Viktoria Tae, Lidwina Felisima Tahulending, Peggy Tea, Marselina Theresia Djue Tefa, Anna tnunay, ite morina yostianti Tode, Sonya Yuniarsi Welsiliana Welsiliana Widhya Faustina Wiguna, Gede Arya Wilda Lumban Tobing Yakobus Pffeferius Edvend Saba Agu Yohana Fatmawati Usfinit Yos Bobi A. Olla Yuliana Kolo Yuni Sine Yuni Sine Zulfah Az Zahra