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Lukas Pardosi
Prodi Biologi, Universitas Timor, Indonesia
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Journal : BIOEDUSCIENCE

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Isolasi Dan Uji Aktivitas Bakteri Proteolitik Yang Berasosiasi Dengan Spons Stylotella Sp. Dan Potensinya Sebagai Antibakteri Seuk, Mariana Septiani; Pardosi, Lukas; Fallo, Gergonius; Manalu, Adelya Irawan
BIOEDUSCIENCE Vol 8 No 1 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12980

Abstract

Background: The province of East Nusa Tenggara (NTT), Indonesia, boasts a significant expanse of marine territory, making its waters a potential treasure trove of aquatic resources. One such marine resource is the Stylotella SP sponge, which is found in this region. Sponges like Stylotella SP often have intricate relationships with microorganisms, including bacteria. Method: This research was to characterize and identify proteolytic bacteria and test bioactive compounds from isolates of proteolytic bacteria from the sponge Stylotella sp. Proteolytic bacterial strains were rejuvenated first in 2% TSA + Nacl medium and then characterized microscopically and biochemically using catalase and oxidase tests. The diffusion method used the antibacterial activity test against E.coli and S. aureus. Result: The results showed that proteolytic bacterial isolates had irregular and rounded colony shapes, wavy and intact colony edges, hilly and convex colony elevation, and white color, while biochemically, it was declared positive for the catalase and oxidase tests. Antibacterial activity tests on proteolytic bacterial strains showed the ability to inhibit the growth of Staphylococcus aureus with an inhibitory capacity of 9.5 mm and Escherichia coli with an inhibitory capacity of 7 mm.
Co-Authors Agustina Yovita Boluk Alfred Nubatonis Anastasia Buak Anggelina Mokos Anjelina M. da Cruz Arkianti, Maria Maxmilla Yoche Azor Yulianus Tefa Banusu, Maria Sarliana Batu, Matius S Benu, Didi Prasetyo Bira, Gerson Frans Bria, Deseriana Charles Venirius Lisnahan Dira Asri Pramita Eduardus Yosef Neonbeni Elmalinda Moensaku Emanuel M. Y. Hano’e Emanuel M. Y. Hano’e Emilia Juliyanti Bria Faesal, Andi Fallo, Gergonius Fitra Ramadhan Florian Mayesti Prima Remba Makin Fobia, Kaprisius G. Fransiskus Yulius Dhewa Kadju Hanas, Dicky Frengky Hano’e, Emanuel M. Y. Hermina Manlea Hermina Manlea I Gede Arya Wiguna Jefry Presson Jefry Presson jefry Presson Jendri Mamangkey Jonris Tampubolon Josua Sahala Jusanty Antoneta Pakh Kamaluddin Kamaluddin Kamaluddin Kamaluddin Kia, Kristoforus W Kia, Kristoforus Wilson Kia, Kristoforus. W. Kolo, Maria Magdalena Korbafo, Elisabeth Kristoforus Wilson Kia Lidya, Christie Liem, Noviana Animra Lisnahan, Charles V. Lusiana Yeni Akoit Mada, Grandianus Seda Manalu, Adelya I Mardit N Nalle Maria Ernalinda Seran Matius Stefanus Batu Meta Permata Putri Metriana Leon Moensaku, Elmalinda Nahak, Oktovianus Rafael Naimuni, Fransiskus Xaverius Natalia Desy Djata Ndua Natalia Hoar Neonbeni, Eduardus Yosef Nugraha K. F. Dethan Pakh, Jusanty Antoneta Pramita, Dira Asri Pramusita, Santa Maya Presson, Jefry Respati Tri Swasono Riska Septia Wahyuningtyas Risna Erni Yati Adu Sabirin Matsjeh Sefrinus Maria Dolfi Kolo Seuk, Mariana Septiani Siahaan, Desta Gloria Sine, Yuni Sio Stefanus, Sio Sipayung, Boanerges Putra SITUMORANG, KOMILIE Tae, Lidwina Felisima Tahulending, Peggy Tea, Marselina Theresia Djue Tefa, Anna tnunay, ite morina yostianti Tode, Sonya Yuniarsi Welsiliana Welsiliana Widhya Faustina Wiguna, Gede Arya Wilda Lumban Tobing Yakobus Pffeferius Edvend Saba Agu Yohana Fatmawati Usfinit Yos Bobi A. Olla Yuliana Kolo Yuni Sine Yuni Sine Zulfah Az Zahra