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All Journal Buletin Peternakan JURNAL KIMIA SAINS DAN APLIKASI Jurnal Pendidikan Biologi Undiksha Jurnal Biologi dan Pembelajarannya (JBP) JURNAL PENGABDIAN KEPADA MASYARAKAT Pro-Life BERITA BIOLOGI Biodidaktika : Jurnal Biologi dan Pembelajarannya Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat BIOEDUSCIENCE Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA JMM (Jurnal Masyarakat Mandiri) MATAPPA: Jurnal Pengabdian Kepada Masyarakat Jurnal Saintek Lahan Kering JPBIO (Jurnal Pendidikan Biologi) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Community Development Journal: Jurnal Pengabdian Masyarakat BERNAS: Jurnal Pengabdian Kepada Masyarakat Bakti Cendana: Jurnal Pengabdian Masyarakat Journal of Tropical Animal Science and Technology Jurnal Pengabdian UNDIKMA Jurnal Biosense Bioscientist : Jurnal Ilmiah Biologi Prosiding Konferensi Nasional PKM-CSR Society: Jurnal Pengabdian Masyarakat Dharma Pengabdian Perguruan Tinggi (DEPATI) JURNAL BIOLOGI PAPUA Jurnal Abdimas Maduma BIO-CONS: Jurnal Biologi dan Konservasi Quagga: Jurnal Pendidikan dan Biologi Journal Science of Biodiversity Journal Science of Biodiversity BIOGENIC: Jurnal Ilmiah Biologi Journal of Chemical Science and Application Berita Biologi
Lukas Pardosi
Prodi Biologi, Universitas Timor, Indonesia
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Journal : Pro-Life

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Aktivitas Mikrobiologis Endofit dari Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis) Jendri Mamangkey; Lukas Pardosi; Riska Septia Wahyuningtyas
Jurnal Pro-Life Vol. 9 No. 1 (2022): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Endophytic bacteria and fungi inhibite the tisues of living plant. Both of them are significant components of plant microecosystems. Current research was performed to explain several microbiological activities (production of extracellular enzymes, antioxidants, and antibacterials) of endophytic bacteria and fungi from binahong leaves extract (Anredera cordifolia (Ten.) Steenis). In this case, the research was conducted by applying a review of several literature reviews published online on Google Scholar and several journals listed on the ScienceDirect site. Based on the results obtained from the research reports from several scientific journal sources, there are four types of extracellular enzymes produced by the endophytic bacteria of the Anredera cordifolia plant, including proteinase, amylase, esterase, and cellulase. In addition, the endophytic bacteria of Pseudomonas aeruginosa from the leaves extract of Anredera cordifolia has antioxidant activity, which is volatile compounds including cis-9-Octadecenoic acid, 2-dodecenoic acid, and 9 octadecenamide produced by endophytic bacteria of Pseudomonas sp. Furthermore, the endophytic bacteria isolated from Anredera cordifolia leaves extract inhibits the growth of E. coli ATCC 35216 and S. aureus ATCC 29523. Meanwhile, the endophytic fungi from Anredera cordifolia leaves extract inhibite the growth of E.coli and S. aureus bacteria. Therefore, understanding the relationship between bacteria and endophytic fungi from the leaves extract of binahong (Anredera cordifolia (Ten.) Steenis) is essential. This knowledge can be utilized and applied well for the future production of higher quality and more medicinal plants from binahong plant (Anredera cordifolia (Ten.) Steenis).
Keanekaragaman Lumut Kerak (Lichenes) di Area Kaki Gunung Mutis Kamaluddin; Emanuel M. Y. Hano’e; Lukas Pardosi
Jurnal Pro-Life Vol. 9 No. 3 (2022): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

The aim of the study was to explore, identify and describe the types of Lichenes in the foothills of Mount Mutis in Eban Village. This research was conducted in April-July 2022 using a qualitative descriptive approach with the reconassance method. Sampling used a purposive sampling technique by taking samples, collecting and collecting them to assist in the laboratory. The results of research that have been carried out by lichens found at the foot of Mount Mutis, Eban Village, West Miomaffo District, amount to 21 types of lichen species with descriptions: Leptogium azureum, Pertusaria sp, Lepraria Loficans, Lepraria incana, Parmelia saxatilis, Parmotrema tinctorum, Cetrelia olivetorum, Heterodermia japonica, Usnea hirta, Parmotrema perlatum, Graphis scripta, Graphis sp 1, Graphis sp 2, Haematomma accolens, Cryptothecia striata, Bacidia schweinitzii, Bacidia sp, Dirinaria applanate, Opegrapha gyrocarpa, Lecidella elaeochroma, Caloplaca ferruginea. The most common lichen groups were the 12 families Parmeliaceae most commonly found in research lines with 5 species, Graphidaceae 4 species, Bacidia 2 species, Stereocaulaceae 2 species, and Collemataceaee, Pertusariaceae, Haematommataceae, Artho-niaceae, Physciaceae, Opegraphaceae, Lecidellaleceae, Teloschistaceae 1 species each. Lichen substrate found living on tree trunks, tree stalks and rocks. The form of lichen thallus that was most commonly found was Crustose which was 62%, then Foliose thallus was 33% and Fruticose was 5%. The diversity of mosses in the study area is strongly influenced by both abiotic and biotic environmental factors. The type of Licen found in Eban Village is a Lichen which is able to adapt to an environment which incidentally is dominated by dry land.
Isolasi dan Uji Patogen Bakteri Asam Laktat (BAL) dari Usus Ayam Kampung yang Dipelihara Secara Intensif Di Kefamenanu Welsiliana; Charles Venirius Lisnahan; Lukas Pardosi
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

This study aimed to isolate LAB from the intestines of native chickens and to test the ability of LAB activity against pathogenic bacteria. Bacterial isolation was carried out by taking chicken intestines, then diluting up to 102. The results of the dilution were taken 1 ml to be inoculated into MRSA media using the pour plate technique, streak plate technique was used to obtain pure cultures and then incubated at 37˚C for 24 hours. LAB culture testing for pathogenic bacteria was carried out using MHA media containing Escherichia coli cultures, then incubation for 120 hours at 37˚C. Observation parameters included colony shape, colony color, number of LAB colonies, the diameter of the clear zone and LAB colonies. The results showed that the bacteria isolated from the intestines of native chickens were LAB which had around colony shape and were yellowish-white (beige)-milky white. The number of LAB colonies obtained was 3.7 x 105 CFU/ml with the best inhibitory ability of pathogenic bacteria found in USA 3 isolates (1.85 cm clear zone; 0.90 cm colony size) after incubation for 120 hours.
Kualitas Mikrobiologi Daging Se’i Sapi melalui Metode Curing menggunakan Ekstrak Daun Jati (Tectona Grandis L.f.) pada Penyimpanan Suhu Ruang Kia, Kristoforus. W.; Fobia, Kaprisius G.; Pardosi, Lukas
Jurnal Pro-Life Vol. 10 No. 2 (2023): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Tanaman jati (Tectona Grandis L.f.) merupakan salah satu tanaman alami jenis pepohonan yang hanya dimanfaatkan batangnya dalam bidang industri, sedangkan daunnya yang memiliki segudang manfaat jarang dimanfaatkan oleh masyarakat sehingga menjadi limbah yang terbuang. Daun jati secara tradisional sering digunakan untuk membungkus bahan makanan seperti daging, ikan dan tempe selama proses fermentasi untuk mencegah terjadinya pembusukan karena mengandung senyawa antibakteri. Penelitian bertujuan mengetahui aktivitas antimikrobial ekstrak daun jati terhadap pertumbuhan bakteri E. coli dan kualitas mikrobiologis daging se’i sapi. Variabel yang diteliti yaitu uji aktivitas antimikrobial ekstrak daun jati terhadap bakteri E. coli, perhitungan jumlah koloni (TPC) dan pengamatan keberadaan bakteri E. coli. Data diolah dengan analisis sidik ragam dan dilanjutkan dengan uji Duncan. Metode penelitian menggunakan Rancangan Acak Lengkap. Perlakuan yang diuji yaitu: P0 = pembuatan dengan penambahan aquades (Kontrol -), P1 = pembuatan se’i dengan penambahan nitrat 0,6 g (kontrol +) dan P2 = pembuatan se’i ditambahkan ekstrak daun jati 0,5%. Hasil menunjukan bahwa konsentrasi ekstrak daun jati 0,5%, 1% dan 1.5% memiliki kemampuan bakteriostatik dengan kategori kuat dan rerata zona bening yang diperoleh yaitu 12.6 mm, 15.4 mm, dan 19.5 mm. Kontrol tidak memiliki kemampuan menghambat pertumbuhan bakteri. Hasil uji mikrobiologi dengan perhitungan TPC menunjukkan bahwa ekstrak daun jati memiliki kemampuan bakteriostatik dengan rerata yang diperoleh 92.0 CFU/g pada konsentrasi 0.5%. Sedangkan pada kontrol (-) dan (+) menunjukan nilai tertinggi yaitu 201.3 CFU/g dan 169.7 CFU/g. Hasil uji cemaran bakteri E. coli menunjukkan bahwa ekstrak daun jati memiliki kemampuan bakteriostatik dengan nilai rerata yang diperoleh 13.3 CFU/g pada konsentrasi 0.5%. Sedangkan pada kontrol (-) dan (+) menunjukan nilai tertinggi yaitu 55.4 CFU/g dan 38.0 CFU/g. Disimpulkan bahwa ekstrak daun jati memiliki kemampuan antimikrobial terhadap bakteri E. coli dengan terbentuknya zona bening pada konsentrasi 0,5%, 1% dan 1.5%, memiliki kemampuan dalam menghambat pertumbuhan koloni bakteri pada se’i sapi. Kata kunci: Antimikrobial, E. coli, ekstrak daun jati, se’i, zona hambat
Co-Authors Agustina Yovita Boluk Alfred Nubatonis Anastasia Buak Anggelina Mokos Anjelina M. da Cruz Arkianti, Maria Maxmilla Yoche Azor Yulianus Tefa Banusu, Maria Sarliana Batu, Matius S Benu, Didi Prasetyo Bira, Gerson Frans Bria, Deseriana Charles Venirius Lisnahan Dira Asri Pramita Eduardus Yosef Neonbeni Elmalinda Moensaku Emanuel M. Y. Hano’e Emanuel M. Y. Hano’e Emilia Juliyanti Bria Faesal, Andi Fallo, Gergonius Fitra Ramadhan Florian Mayesti Prima Remba Makin Fobia, Kaprisius G. Fransiskus Yulius Dhewa Kadju Hanas, Dicky Frengky Hano’e, Emanuel M. Y. Hermina Manlea Hermina Manlea I Gede Arya Wiguna Jefry Presson Jefry Presson jefry Presson Jendri Mamangkey Jonris Tampubolon Josua Sahala Jusanty Antoneta Pakh Kamaluddin Kamaluddin Kamaluddin Kamaluddin Kia, Kristoforus W Kia, Kristoforus Wilson Kia, Kristoforus. W. Kolo, Maria Magdalena Korbafo, Elisabeth Kristoforus Wilson Kia Lidya, Christie Liem, Noviana Animra Lisnahan, Charles V. Lusiana Yeni Akoit Mada, Grandianus Seda Manalu, Adelya I Mardit N Nalle Maria Ernalinda Seran Matius Stefanus Batu Meta Permata Putri Metriana Leon Moensaku, Elmalinda Nahak, Oktovianus Rafael Naimuni, Fransiskus Xaverius Natalia Desy Djata Ndua Natalia Hoar Neonbeni, Eduardus Yosef Nugraha K. F. Dethan Pakh, Jusanty Antoneta Pramita, Dira Asri Pramusita, Santa Maya Presson, Jefry Respati Tri Swasono Riska Septia Wahyuningtyas Risna Erni Yati Adu Sabirin Matsjeh Sefrinus Maria Dolfi Kolo Seuk, Mariana Septiani Siahaan, Desta Gloria Sine, Yuni Sio Stefanus, Sio Sipayung, Boanerges Putra SITUMORANG, KOMILIE Tae, Lidwina Felisima Tahulending, Peggy Tea, Marselina Theresia Djue Tefa, Anna tnunay, ite morina yostianti Tode, Sonya Yuniarsi Welsiliana Welsiliana Widhya Faustina Wiguna, Gede Arya Wilda Lumban Tobing Yakobus Pffeferius Edvend Saba Agu Yohana Fatmawati Usfinit Yos Bobi A. Olla Yuliana Kolo Yuni Sine Yuni Sine Zulfah Az Zahra