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Antioxidant, Cytotoxic Activities and Total Phenolic Content of Four Indonesian Medicinal Plants Waras Nurcholis; Bambang Pontjo Priosoeryanto; Edy Djauhari Purwakusumah; Takeshi Katayama; Toshisada Suzuki
Jurnal Kimia Valensi Jurnal Valensi Volume 2, No.4, Mei 2012
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.104 KB) | DOI: 10.15408/jkv.v2i4.267

Abstract

The crude ethanol extracts of four Indonesian medicinal plants namely Curcuma xanthorrhiza Roxb.,Phyllanthus niruri Linn., Andrographis paniculata Ness., and Curcuma aeruginosa Roxb. wereexamined for their antioxidant (radical scavenging) activity using 2, 2-diphenyl-2-picrylhydrazyl(DPPH) free radical and cytotoxicity using brine shrimp lethality test (BSLT). The total phenoliccontent was used the Folin-Ciocalteu method. IC50 values for DPPH radical scavenging activityranged from 14.5 to 178.5 μg/ml, with P. niruri having the lowest value and therefore the mostpotent, and C. aeruginosa having the highest value. LC50 values for BSLT ranged from 210.3 to593.2 μg/ml, with C. xanthorrhiza and A. paniculata having the lowest and highest values,respectively. The total phenolic content of the Indonesian plants ranged from 133.0 ±3.7 to863.3±54.7 mg tannic acid equivalent per 1 g extract, with C. aeruginosa and P. niruri having thelowest and highest values, respectively. A positive correlation between free radical scavengingactivity and the content of phenolic compounds was found in the four of Indonesian medicinal plants.
Characteristics of Glucose Oxidase Gene (GGOx) from Aspergillus niger IPBCC 08.610 Popi Asri Kurniatin; Laksmi Ambarsari; Annisa Dhiya Athiyyah Khanza; Inda Setyawati; Djarot Sasongko Hami Seno; Waras Nurcholis
Jurnal Kimia Valensi Jurnal Kimia VALENSI Volume 6, No. 1, May 2020
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (763.755 KB) | DOI: 10.15408/jkv.v6i1.9440

Abstract

Glucose oxidase is used in various industries for the development of enzymatic fuel cell. Based on prior studies, this compound is sourced from the local isolates of Aspergillus niger IPBCC 08.610, although investigations on the encoding gene have not been conducted. The purpose of this research, therefore, is to identify and characterized the gene responsible for encoding glucose oxidase, in the aspect of sequence, length, and restriction patterns. This experiment involved the amplification of genomic DNA using specific primers for gene recognition, which was followed by the restriction technique with EcoRI and PstI endonucleases. Furthermore, the gene is inserted into vector pGEM®T-Easy and transformed into competent E. coli DH5α cells, in an attempt to perform sequencing. The glucose oxidase gene from A. niger IPBCC 08.610 was confirmed to possess a size of 1848 bp, and a GC content of 57.8%, with a possibility of restriction into two fragments of size 908 bp and 980 bp, using the EcoRI restriction.
Phytochemical Analysis, α-glucosidase Inhibition Activity in-vitro and Enzyme Kinetics of Ethyl Acetate and Hexane Extracts of Graptophylum pictum (L.) Griff Waras Nurcholis; I Made Artika; Djarot Sasongko Hami Seno; Dimas Andrianto; Apipah Aprianti; Fina Febrianti; Inawati Inawati; Antonius Padua Ratu; Arya Arendra
Current Biochemistry Vol. 1 No. 2 (2014)
Publisher : IPB University

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Abstract

The species Graptophylum pictum (L.) Griff, also known as “daun ungu” in Indonesia, is a traditional herbaceous plant believed to have antidiabetic potential. The number of people in the world with diabetes has increased dramatically over the recent years. The treatment of type II diabetes is complicated by several factors inherent to the disease. Elevated postprandial hyperglycemia is one of the risk factors and the intestinal digestive enzyme α-glucosidase plays a vital role in carbohydrate metabolism. One of the antidiabetic therapeutic approaches which reduces the postprandial glucose level in blood is by the inhibition of α-glucosidase. In this study, phytochemical analysis, α- glucosidase inhibitory activity and enzyme kinetics of ethyl acetate- and hexane extracts of G. pictum were evaluated with the aim to analyze its antidiabetic potential. Phytochemical analysis revealed the presence of tannins, steroids, and alkaloids. Steroids were present in ethyl acetate extract but absent in hexane extract, while alkaloids were present in hexane extract but absent in ethyl acetate extract. The ethyl acetate and hexane extracts had 30.68 and 49.82 % inhibitory effect on α-glucosidase activity respectively. The kinetics of glucosidase enzyme of ethyl acetate and hexane extracts were determined by Lineweaver Burk plots. These exhibited uncompetitive and noncompetitive inhibition to alpha-glucosidase activity respectively. From the enzyme assay, we infer that ethyl acetate and hexane extracts of G. pictum contain potential α-glucosidase inhibitors that have the potential to be exploited for use in the treatment of diabetes
Bioethanol Production by Using Detoxified Sugarcane Bagasse Hydrolysate and Adapted Culture of Candida tropicalis Inda Setyawati; Laksmi Ambarsari; Siti Nur'aeni; Suryani Suryani; Puspa Julistia Puspita; Popi Asri Kurniatin; Waras Nurcholis
Current Biochemistry Vol. 2 No. 1 (2015)
Publisher : IPB University

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Abstract

Ethanol is considered as the most promising alternative fuel, since it can be produced from a variety of agriculturally-based renewable materials, such as sugarcane bagasse. Lignocellulose as a major component of sugarcane bagasse is considered as an attractive renewable resource for ethanol production due to its great availability and relatively low cost. The major problem of lignocellulose is caused by its need for treatment to be hydrolyzed to simple sugar before being used for bioethanol production. However, pretreatment using acid as hydrolyzing agent creates some inhibitor compounds that reduce ethanol production because these compounds are potential fermentation inhibitors and affect the growth rate of the yeast. Reduction of these by-products requires a conditioning (detoxification and culture starter adaptation). Thus, the aim of this study was to evaluate bioethanol production by fermentation with and without detoxified sugarcane bagasse acid hydrolysate using adapted and non-adapted culture of C. tropicalis. According to this study, the highest ethanol amount was obtained about 0.43 % (v/v) with an ethanol yield of 2.51 % and theoretical yield of 4.92 % by fermentation of sugarcane bagasse hydrolysate with detoxification using the adapted strain of C. tropicalis at 72 hours fermentation time. Furthermore, the addition of 3 % glucose as co-substrate on detoxified-hydrolysate media only achieved the highest ethanol concentration 0.21 % after 24 hours fermentation with the ethanol yield 0.69 % and theoretical ethanol yield 1.35 %, thus it can be concluded that the addition of glucose could not increase the ethanol production.
Anti-inflammatory Activity of Temulawak Nanocurcuminoid Coated with Palmitic Acid in The Sprague Dawley Rat Rini Novita; Laksmi Ambarsari; Syamsul Falah; Popi Asri Kurniatin; Waras Nurcholis; Latifah K Darusman
Current Biochemistry Vol. 2 No. 2 (2015)
Publisher : IPB University

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Abstract

Temulawak or Curcuma xanthorrhiza Roxb, is usually used as traditional medicine (herbal medicine) that has antioxidant, anticancer, antihyperglycemic, anti-inflammatory, hepatoprotective and gastroprotective properties. The main components contained in temulawak responsible for its efficacy as a medicine are xantorhizol and curcuminoid. Curcuminoid has drawbacks, which are difficult to absorb and very quickly metabolized by the body, so that limit its bioavailability. The use of solid lipid nanoparticle carrier system (SLN) in form of palmitic acid, is known to improve the bioavailability of curcuminoid. This study aims to find the effective dose of nanocurcuminoid coated with palmitic acid that can be used as an anti-inflammatory agent. The methods used in this study, include the production of nanocurcuminoid with homogenization and ultrasonication methods, determination of particle size, polydispersity index, entrapment efficiency and anti-inflammatory activity test through rat feet edema. Nanocurcuminoid obtained in this study was 561.53 nm in size, with polydispersity index 0.309 and concentrations of curcuminoid absorbed and entrapment efficiency were 0.61±0.031 mg/mL, 58.93±3.021%, respectively. Anti-inflammatory activity of nanocurcuminoid through treated Sprague Dawley rats, showed that there were no significant difference compared with the positive control, curcuminoid extracts and empty nanoparticle. These results indicate that nanocurcuminoid with 175, 200 and 250 mg/kg.bw in doses, has greater anti-inflammatory activity (31.70%) compared to the other treatments.
The Activity of Wungu Leaf (Graptophyllum pictum (L) Griff) Extract in Reducing Blood Glucose Level of Hyperglycemic Mice Hayatul Rahmi; I Made Artika; Norman Razief Azwar; Djarot Sasongko Hami Seno; Waras Nurcholis
Current Biochemistry Vol. 1 No. 2 (2014)
Publisher : IPB University

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Abstract

Wungu leaf (Graptophyllum pictum (L.) Griff) is a plant thought to have potential use in alleviating symptoms of diabetes mellitus. The purpose of the present study was to evaluate the activity of wungu leaf extracts in decreasing blood glucose level of alloxan (200 mg/kg BW)-induced hyperglycemic mice. Extracts of wungu leaf were obtained by macerating with ethanol and then partitioning the extract with diethyl ether, ethyl acetate, and butanol. Each extract obtained was used to treat hyperglycemic mice for 28 days. The results showed that wungu leaf extracts have the ability to decrease the blood glucose level of hyperglycemic mice (dose 50 mg/kg BW). The ethyl acetate extract showed the highest activity, bringing about a decrease of blood glucose of 37.6 %. The wungu leaf extract has the potential to be developed as a source of anti-diabetic agents.
Characterization and Toxicity of Temulawak Curcuminoid Nanoparticles Riki Riki; Popi Asri Kurniatin; Laksmi Ambarsari; waras Nurcholis; Latifah K Darusman
Current Biochemistry Vol. 3 No. 1 (2016)
Publisher : IPB University

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Abstract

Temulawak exctract contains curcuminoids which have anticancer potential. However, clinical application of curcuminoid has been limited due to its low bioavailability. One of the efforts that can be developed to solve this problem is incorporated curcuminoids into Solid Lipid Nanoparticles (SLN) carriers system. The objective of this study was to characterize dan evaluate anticancer potential of temulawak ethanolic fraction nanoparticles. HPLC method was used to determined curcuminoids content of temulawak ethanolic fraction. Characterization indicators like polydispersity index, particle size, morpholgy, and entrapment efficiency. HPLC chromatogram has shown of curcumin, demethoxycurcumin, and bisdemethoxycurcumin were found in temulawak ethanolic fraction. The particle size of nanoparticles obtained in this study was 648.4 ± 95 nm with polydispersity index value of 0.216. A uniform size distribution of nanoparticles as observed by Transmission Electron Microscopy (TEM). The entrapment efficiency of curcuminoid in nanoparticles was about 29.8%. Based on results of BSLT obtained temulawak extract Lethal Concentration (LC50) value of 213.24 ppm and 828.78 ppm of nanoparticles.
Aktivitas Antioksidan Ekstrak Etanol dari akar, batang, dan daun Sereh Wangi (Cymbopogon nardus L) Waras Nurcholis; Hartanti Hartanti; Hyakansa Hyakansa; Maria Magdalena; Tubagus Iqbal Maulana
Current Biochemistry Vol. 4 No. 2 (2017)
Publisher : IPB University

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Abstract

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Inhibition of α-glucosidase Activity Seed Extract from Mahogany (Swietenia macrophylla King.) Waras Nurcholis; Rini Muthoharoh; Antonius Padua Ratu
Current Biochemistry Vol. 6 No. 1 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.1.7

Abstract

Diabetes mellitus is a chronic disorder that affects the metabolism of blood glucose inside the body. One of the alternative treatments is by using traditional medicinal plants, which have hypoglycemia effects. A mahogany seed is one of the traditional medicinal plants that has been proven to treat diabetes and has been used for generations. In the aqueous extract, ethanol 96 %, ethyl acetate, and hexane from mahogany seeds (Swietenia macrophylla King.) the inhibition of α-glucosidase activity are checked by measuring p-nitrophenol from p-nitrophenyl-α-D-glucopyranose (pNPG) on 400 nm. The percentage value of the inhibition for α-glucosidase ranged from 6,36-56,77 %, with 96 % ethanol extract that has the highest value and the hexane extract with the lowest value. Phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, and triterpenoids, which could be responsible for the bioactivities shown by the 96 % ethanol extract and aqueous extract of S. macrophylla. Keywords : α-glucosidase activity, diabetes mellitus, mahogany seeds (Swietenia macrophylla King.), phytochemical screening.
Antibacterial Activity of Lemongrass (Cymbopogon nardus) Ethanolic Extract Waras Nurcholis; Mira Takene; Ratna Puspita; Lisnawati Tumanggor; Eka Nurul Qomaliyah; Muhammad Maftuchin Sholeh
Current Biochemistry Vol. 6 No. 2 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.2.4

Abstract

Infectious diseases are a disease caused by bacteria such as Escherichia coli and Staphylococcus aureus. One way to overcome the disease by using antibiotics. However, wise use can lead to antibiotic resistance, so many bacteria are resistant to antibiotics. Therefore it is necessary to search the natural compounds as antibacterials, one of them by using lemongrass (Cymbopogon nardus). This study aims to determine the effectiveness of leaf extracts, stems, and roots lemongrass as antibacterial. The method used in this research is the disc method. Bacteria used are E. coli and S. aureus. The results show extracts of roots, stems, and leaf has activity against S. aureus, while E. coli shows no activity as antibacterial, it can be possible because of the different properties of the bacteria. Keywords: anti-bacteria, lemongrass, Escherichia coli, Staphylococcus aureus
Co-Authors A.A. Ketut Agung Cahyawan W AA Sudharmawan, AA Alfari Andiqa Muhammad Ali Umar Amalia Prihaningsih Anggriawan, Made B. Ani Kurniawati Annisa Dhiya Athiyyah Khanza Antonius Padua Ratu Antonius Padua Ratu Anyalirria Anyalirria Apipah Aprianti Arianti, Rini Arya Arendra Asy Syifa Tickna Maulana Aulia Taureza Baharuddin Yusuf Habibie Bambang Pontjo Priosoeryanto Binukajati, Farras Agung Chelsea Chelsea, Chelsea Damarhati, Lelly Aulia Dara Juliana Dewi Sukma Dian Apriliani, Dian Dian R. Laksmitawati DIMAS ANDRIANTO Dita Audia Putri Djarot Sasongko Hami Seno Dyah Sulistyani Rahayu Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Egra, Saat Eka Nurul Qomaliyah Enny Rimita Sembiring Erna Subroto Eva Harlina Fachrur Rizal Mahendra Fachrur Rizal Mahendra Fadila Raisyadikara Faizal Maulana Fajriansyah, Muhamad Renza Faris Adam Farras Agung Binukajati Fina Febrianti Firmanul Hasan Fitria, Rizki Fransiska Devy Junardy GIA PERMASKU Gultom, Milanda Fiorella Habibi, B Y Hamdan Hafizh Harahap Hanifah Aryani Hartanti Hartanti Hartanti Hartanti Hartanti Hartanti, Hartanti Hayatul Rahmi Hyakansa Hyakansa I Made Artika I MADE ARTIKA Inawati Inawati Inda Setyawati, Inda Innes Maulidya Irmanida Batubara Juliana, Dara Junardy, Fransiska Devy Kamilah Da’inawari Khalissa Sekar Amanda Sudarjat Kilala Tilaar Kornelia Rosvita Manek Kunto Wibisono Kunto Wibisono Kunto Wibisono LAKSMI AMBARSARI Latifah K Darusman Latifah K Darusman Latifah K. Darusman Lelly Aulia Damarhati Lelly Aulia Damarhati Lina Noviyanti Sutardi Lisnawati Tumanggor Liwanda, Novian Lucky Lia Faiza Luky Adrianto M, Kornelia Rosvita M. Agung Zaim Adzkiya M. Rafi Maily Mustofa MARIA BINTANG Maria Bintang Maria Magdalena Maulana, Asy Syifa Tickna Mayang Anggita Cahya Kurnia Milanda Fiorella Gultom Milanda Fiorella Gultom Mira Takene Mohamad Rafi Mono Rahardjo Muhamad Renza Fajriansyah Muhamad Rizal Martua Damanik Muhamad Syukur Muhammad Maftuchin Sholeh Muhammad Musthofa Muhammad Riyo Agung Kurnia Mustika Weni Mustofa, Maily Muthoharoh, Rini Najmah Najmah Najmah Najmah, Najmah Nella Angelina Simanjuntak Nelly Marliani Nevianti Zamany Ning Harmanto Norman Razief Azwar Novian Liwanda Novian Liwanda Novita Sari Nurul Khumaida Nurul Komala P.Roswiem, Anna Pebra Heriansyah Popi Asri Kurniatin Poppy F. Arifin Priyadi, Yogo Suro Puspa Julistia Puspita Putra, Raihan Permana Putri, Dita Audia Qomariyah, Eka Nurul Rachmi Ridho Rahmadansah Rahmadansah Raihan Permana Putra Raphael Aswin Susilowidodo Rara Annisaur Rosyidah Ratna Puspita Ratna Puspita, Ratna Rerenstradika Tizar Terryana Riki Riki Rikno Harmoko Rini Arianti Rini Muthoharoh Rini Novita Riski Rostantinata Riti, Dominggus Ngongo Riyan Alifbi Putera Irsal Rizki Fitria Rizki Fitria Ros Sumarny Rosalina Wisastra Roswiem, Anna Priangani Rusmiyati, Henny Saat Egra Sadwika Najmi Kautsari Safira Khoirunnisa Sandra Arifin Aziz Seliani Fitria Mukti Shobiroh Nuur' Alimah Sholeh, Muhammad Maftuchin Siahaan Rafael Siti Nur'aeni Sri Suhesti Sri Suhesti Sri Suhesti Stephanie Sriwongo Subroto, Erna Sukma, Dewi Suryani Suryani Suryani Suryani Suryani Suryani Syamsul Falah Syarifah Iis Aisyah Takene, Mira Takeshi Katayama Taopik Ridwan Taufik Ridwan Taufik Ridwan Teti Mulyati Tiara Rizky Utami Tilaar, Kilala Tony Sumaryada Toshisada Suzuki Tubagus Iqbal Maulana Tumanggor, Lisnawati Utami Dyah Safitri Weni, Mustika Wulan Tri Wahyuni Wulansari, Laela Yogo Suro Priyadi Yoshua Shandy Yudha Yuliana Galih Dyan Anggraheni Yulianto Zamany, Nevianti