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All Journal International Journal of Advances in Applied Sciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Hemera Zoa Vegetalika VALENSI Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL KIMIA SAINS DAN APLIKASI Current Biochemistry AGRIVITA, Journal of Agricultural Science agriTECH Indonesian Journal of Chemistry Agrin : Jurnal Penelitian Pertanian Justek : Jurnal Sains Dan Teknologi Biosaintifika: Journal of Biology & Biology Education Media Farmasi Journal of Tropical Crop Science AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Molekul: Jurnal Ilmiah Kimia Jurnal Ilmu Kefarmasian Indonesia Jurnal Jamu Indonesia YARSI Medical Journal Poltekita : Jurnal Ilmu Kesehatan Jurnal Farmamedika (Pharmamedica Journal) Indonesian Journal of Contemporary Multidisciplinary Research Indonesian Journal of Jamu
Waras Nurcholis
Departemen Biokimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor (IPB University), Jl. Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Phytochemical Analysis, α-glucosidase Inhibition Activity in-vitro and Enzyme Kinetics of Ethyl Acetate and Hexane Extracts of Graptophylum pictum (L.) Griff Waras Nurcholis; I Made Artika; Djarot Sasongko Hami Seno; Dimas Andrianto; Apipah Aprianti; Fina Febrianti; Inawati Inawati; Antonius Padua Ratu; Arya Arendra
Current Biochemistry Vol. 1 No. 2 (2014)
Publisher : IPB University

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Abstract

The species Graptophylum pictum (L.) Griff, also known as “daun ungu” in Indonesia, is a traditional herbaceous plant believed to have antidiabetic potential. The number of people in the world with diabetes has increased dramatically over the recent years. The treatment of type II diabetes is complicated by several factors inherent to the disease. Elevated postprandial hyperglycemia is one of the risk factors and the intestinal digestive enzyme α-glucosidase plays a vital role in carbohydrate metabolism. One of the antidiabetic therapeutic approaches which reduces the postprandial glucose level in blood is by the inhibition of α-glucosidase. In this study, phytochemical analysis, α- glucosidase inhibitory activity and enzyme kinetics of ethyl acetate- and hexane extracts of G. pictum were evaluated with the aim to analyze its antidiabetic potential. Phytochemical analysis revealed the presence of tannins, steroids, and alkaloids. Steroids were present in ethyl acetate extract but absent in hexane extract, while alkaloids were present in hexane extract but absent in ethyl acetate extract. The ethyl acetate and hexane extracts had 30.68 and 49.82 % inhibitory effect on α-glucosidase activity respectively. The kinetics of glucosidase enzyme of ethyl acetate and hexane extracts were determined by Lineweaver Burk plots. These exhibited uncompetitive and noncompetitive inhibition to alpha-glucosidase activity respectively. From the enzyme assay, we infer that ethyl acetate and hexane extracts of G. pictum contain potential α-glucosidase inhibitors that have the potential to be exploited for use in the treatment of diabetes
Bioethanol Production by Using Detoxified Sugarcane Bagasse Hydrolysate and Adapted Culture of Candida tropicalis Inda Setyawati; Laksmi Ambarsari; Siti Nur'aeni; Suryani Suryani; Puspa Julistia Puspita; Popi Asri Kurniatin; Waras Nurcholis
Current Biochemistry Vol. 2 No. 1 (2015)
Publisher : IPB University

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Abstract

Ethanol is considered as the most promising alternative fuel, since it can be produced from a variety of agriculturally-based renewable materials, such as sugarcane bagasse. Lignocellulose as a major component of sugarcane bagasse is considered as an attractive renewable resource for ethanol production due to its great availability and relatively low cost. The major problem of lignocellulose is caused by its need for treatment to be hydrolyzed to simple sugar before being used for bioethanol production. However, pretreatment using acid as hydrolyzing agent creates some inhibitor compounds that reduce ethanol production because these compounds are potential fermentation inhibitors and affect the growth rate of the yeast. Reduction of these by-products requires a conditioning (detoxification and culture starter adaptation). Thus, the aim of this study was to evaluate bioethanol production by fermentation with and without detoxified sugarcane bagasse acid hydrolysate using adapted and non-adapted culture of C. tropicalis. According to this study, the highest ethanol amount was obtained about 0.43 % (v/v) with an ethanol yield of 2.51 % and theoretical yield of 4.92 % by fermentation of sugarcane bagasse hydrolysate with detoxification using the adapted strain of C. tropicalis at 72 hours fermentation time. Furthermore, the addition of 3 % glucose as co-substrate on detoxified-hydrolysate media only achieved the highest ethanol concentration 0.21 % after 24 hours fermentation with the ethanol yield 0.69 % and theoretical ethanol yield 1.35 %, thus it can be concluded that the addition of glucose could not increase the ethanol production.
Anti-inflammatory Activity of Temulawak Nanocurcuminoid Coated with Palmitic Acid in The Sprague Dawley Rat Rini Novita; Laksmi Ambarsari; Syamsul Falah; Popi Asri Kurniatin; Waras Nurcholis; Latifah K Darusman
Current Biochemistry Vol. 2 No. 2 (2015)
Publisher : IPB University

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Abstract

Temulawak or Curcuma xanthorrhiza Roxb, is usually used as traditional medicine (herbal medicine) that has antioxidant, anticancer, antihyperglycemic, anti-inflammatory, hepatoprotective and gastroprotective properties. The main components contained in temulawak responsible for its efficacy as a medicine are xantorhizol and curcuminoid. Curcuminoid has drawbacks, which are difficult to absorb and very quickly metabolized by the body, so that limit its bioavailability. The use of solid lipid nanoparticle carrier system (SLN) in form of palmitic acid, is known to improve the bioavailability of curcuminoid. This study aims to find the effective dose of nanocurcuminoid coated with palmitic acid that can be used as an anti-inflammatory agent. The methods used in this study, include the production of nanocurcuminoid with homogenization and ultrasonication methods, determination of particle size, polydispersity index, entrapment efficiency and anti-inflammatory activity test through rat feet edema. Nanocurcuminoid obtained in this study was 561.53 nm in size, with polydispersity index 0.309 and concentrations of curcuminoid absorbed and entrapment efficiency were 0.61±0.031 mg/mL, 58.93±3.021%, respectively. Anti-inflammatory activity of nanocurcuminoid through treated Sprague Dawley rats, showed that there were no significant difference compared with the positive control, curcuminoid extracts and empty nanoparticle. These results indicate that nanocurcuminoid with 175, 200 and 250 mg/kg.bw in doses, has greater anti-inflammatory activity (31.70%) compared to the other treatments.
The Activity of Wungu Leaf (Graptophyllum pictum (L) Griff) Extract in Reducing Blood Glucose Level of Hyperglycemic Mice Hayatul Rahmi; I Made Artika; Norman Razief Azwar; Djarot Sasongko Hami Seno; Waras Nurcholis
Current Biochemistry Vol. 1 No. 2 (2014)
Publisher : IPB University

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Abstract

Wungu leaf (Graptophyllum pictum (L.) Griff) is a plant thought to have potential use in alleviating symptoms of diabetes mellitus. The purpose of the present study was to evaluate the activity of wungu leaf extracts in decreasing blood glucose level of alloxan (200 mg/kg BW)-induced hyperglycemic mice. Extracts of wungu leaf were obtained by macerating with ethanol and then partitioning the extract with diethyl ether, ethyl acetate, and butanol. Each extract obtained was used to treat hyperglycemic mice for 28 days. The results showed that wungu leaf extracts have the ability to decrease the blood glucose level of hyperglycemic mice (dose 50 mg/kg BW). The ethyl acetate extract showed the highest activity, bringing about a decrease of blood glucose of 37.6 %. The wungu leaf extract has the potential to be developed as a source of anti-diabetic agents.
Characterization and Toxicity of Temulawak Curcuminoid Nanoparticles Riki Riki; Popi Asri Kurniatin; Laksmi Ambarsari; waras Nurcholis; Latifah K Darusman
Current Biochemistry Vol. 3 No. 1 (2016)
Publisher : IPB University

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Abstract

Temulawak exctract contains curcuminoids which have anticancer potential. However, clinical application of curcuminoid has been limited due to its low bioavailability. One of the efforts that can be developed to solve this problem is incorporated curcuminoids into Solid Lipid Nanoparticles (SLN) carriers system. The objective of this study was to characterize dan evaluate anticancer potential of temulawak ethanolic fraction nanoparticles. HPLC method was used to determined curcuminoids content of temulawak ethanolic fraction. Characterization indicators like polydispersity index, particle size, morpholgy, and entrapment efficiency. HPLC chromatogram has shown of curcumin, demethoxycurcumin, and bisdemethoxycurcumin were found in temulawak ethanolic fraction. The particle size of nanoparticles obtained in this study was 648.4 ± 95 nm with polydispersity index value of 0.216. A uniform size distribution of nanoparticles as observed by Transmission Electron Microscopy (TEM). The entrapment efficiency of curcuminoid in nanoparticles was about 29.8%. Based on results of BSLT obtained temulawak extract Lethal Concentration (LC50) value of 213.24 ppm and 828.78 ppm of nanoparticles.
Aktivitas Antioksidan Ekstrak Etanol dari akar, batang, dan daun Sereh Wangi (Cymbopogon nardus L) Waras Nurcholis; Hartanti Hartanti; Hyakansa Hyakansa; Maria Magdalena; Tubagus Iqbal Maulana
Current Biochemistry Vol. 4 No. 2 (2017)
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Inhibition of α-glucosidase Activity Seed Extract from Mahogany (Swietenia macrophylla King.) Waras Nurcholis; Rini Muthoharoh; Antonius Padua Ratu
Current Biochemistry Vol. 6 No. 1 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.1.7

Abstract

Diabetes mellitus is a chronic disorder that affects the metabolism of blood glucose inside the body. One of the alternative treatments is by using traditional medicinal plants, which have hypoglycemia effects. A mahogany seed is one of the traditional medicinal plants that has been proven to treat diabetes and has been used for generations. In the aqueous extract, ethanol 96 %, ethyl acetate, and hexane from mahogany seeds (Swietenia macrophylla King.) the inhibition of α-glucosidase activity are checked by measuring p-nitrophenol from p-nitrophenyl-α-D-glucopyranose (pNPG) on 400 nm. The percentage value of the inhibition for α-glucosidase ranged from 6,36-56,77 %, with 96 % ethanol extract that has the highest value and the hexane extract with the lowest value. Phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, and triterpenoids, which could be responsible for the bioactivities shown by the 96 % ethanol extract and aqueous extract of S. macrophylla. Keywords : α-glucosidase activity, diabetes mellitus, mahogany seeds (Swietenia macrophylla King.), phytochemical screening.
Antibacterial Activity of Lemongrass (Cymbopogon nardus) Ethanolic Extract Waras Nurcholis; Mira Takene; Ratna Puspita; Lisnawati Tumanggor; Eka Nurul Qomaliyah; Muhammad Maftuchin Sholeh
Current Biochemistry Vol. 6 No. 2 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.2.4

Abstract

Infectious diseases are a disease caused by bacteria such as Escherichia coli and Staphylococcus aureus. One way to overcome the disease by using antibiotics. However, wise use can lead to antibiotic resistance, so many bacteria are resistant to antibiotics. Therefore it is necessary to search the natural compounds as antibacterials, one of them by using lemongrass (Cymbopogon nardus). This study aims to determine the effectiveness of leaf extracts, stems, and roots lemongrass as antibacterial. The method used in this research is the disc method. Bacteria used are E. coli and S. aureus. The results show extracts of roots, stems, and leaf has activity against S. aureus, while E. coli shows no activity as antibacterial, it can be possible because of the different properties of the bacteria. Keywords: anti-bacteria, lemongrass, Escherichia coli, Staphylococcus aureus
Toxicity Test of Roots, Stems and Leaves of Lemongrass (Cymbopogon nardus) Waras Nurcholis; Mustika Weni; Rizki Fitria; Najmah; Kornelia Rosvita Manek; Baharuddin Yusuf Habibie
Current Biochemistry Vol. 6 No. 2 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.2.6

Abstract

Lemongrass traditionally can be used as a mixture of water bath for rheumatic patients, antiseptic medicine, headache reducer, unbend the leech bite and overcome insect bite, but, the bioactivity potential of citronella lemongrass still remains unknown. The content of citronella lemongrass is known to have secondary metabolites such as flavanoid, phenolic, and terpenoid. The aim of this study is to determine the LC50 value (Lethality Concentration 50) in the root, stem, and leaves part of citronella lemongrass. Citronella lemongrass is extracted with maceration method using 96% ethanol solvent. Rendement value of leaf extract is greater than the value of stem and root extract, which is equal to 8.69% for stem extract, 3.73% for root extract and 9.70% for leaves extract. A toxicity test was performed by using the BSLT method, shrimp larvae were inserted into the test solution with each different solution concentration. LC50 values were obtained based on the calculation of percent mortality of shrimp larvae by using probit analysis. LC50 ethanol extract 96% of citronella lemongrass leaf is 67.841 μg/ml, citronella lemongrass stem is 31.604 μg/ml and citronella lemongrass root is 10.489 μg/ml. LC50 value shows that ethanol extract 96% of root, stem, and leaf of citronella lemongrass have no bioactivity potential due to the value of LC50 is greater than 1000 μg/ml. Keywords: BSLT, Cymbopogon nardus, cytotoxicity, LC50
The Potency of Nanocurcuminoid of Temulawak as A Preventive Agent for Lipid Peroxidation on Inflammation in Rats Waras Nurcholis; Chelsea; Laksmi Ambarsari
Current Biochemistry Vol. 6 No. 1 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.1.5

Abstract

The formation of prostaglandins and free radicals in the body occurs in response to inflammation. The use of non-steroidal synthetic drugs to overcome the effects of free radicals often causes harmful side effects to the body. Natural ingredients that have high antioxidant potential, such as curcumin, ginger are expected to overcome this problem. This study aims to measure the antioxidant potential of ginger nanocurcuminoid preparations coated with palmitic acid, by observing the lipid peroxide profile in the liver of inflammatory Spague-Dawley rats. SpagueDawley mice were induced with 1% carrageenan to trigger inflammation, then the lipid peroxide levels were measured after 24 hours. Measurement of lipid peroxide levels was carried out using a spectrophotometer with a wavelength of 532 nm. The size of the nanocurcuminoid preparation coated with palmitic acid was 561.53 nm with an IP value of 0.309. Lipid peroxide levels in the curcuminoid extract group at a dose of 100 mg / kg BW and the nanocurcuminoid group at a dose of 250 mg / kg BW were 0.27x10-4 nmol / g and 1.22 x10-4 nmol / g respectively. These showed that the antioxidant potential of temulawak nanocurcuminoids at a dose of 250 mg / kg BW was 114 times higher than that of curcuminoid extract at a dose of 100 mg / kg BW.
Co-Authors A.A. Ketut Agung Cahyawan W AA Sudharmawan, AA Alfari Andiqa Muhammad Ali Umar Amalia Prihaningsih Anggriawan, Made B. Ani Kurniawati Annisa Dhiya Athiyyah Khanza Antonius Padua Ratu Antonius Padua Ratu Anyalirria Apipah Aprianti Arianti, Rini Arifin, Poppy F. Arya Arendra Baharuddin Yusuf Habibie Bambang Pontjo Priosoeryanto Bintang, dan Maria Binukajati, Farras Agung Chelsea Chelsea, Chelsea Damarhati, Lelly Aulia Dewi Sukma Dian Apriliani, Dian Dian R. Laksmitawati DIMAS ANDRIANTO Djarot Sasongko Hami Seno Dolfina Costansah Koirewoa Dyah Sulistyani Rahayu Edoward Krisson Raunsay Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Egra, Saat Eka Nurul Qomaliyah Enny Rimita Sembiring Erna Subroto Eva Harlina Fachrur Rizal Mahendra Fachrur Rizal Mahendra Fachrur Rizal Mahendra Fadila Raisyadikara Faiza, Lucky Lia Faizal Maulana Fajriansyah, Muhamad Renza Faris Adam Fina Febrianti Firmanul Hasan Fitria, Rizki Fransiska Devy Junardy Ghifari, Muhammad Nabil GIA PERMASKU Gultom, Milanda Fiorella Habibi, B Y Hamdan Hafizh Harahap Hamdan Hafizh Harahap Hanifah Aryani Harmanto, Ning Hartanti Hartanti Hartanti Hartanti Hartanti Hartanti, Hartanti Hayatul Rahmi Hyakansa Hyakansa I Made Artika I MADE ARTIKA Inawati Inawati Inda Setyawati, Inda Innes Maulidya Irmanida Batubara Jesajas, David Reinhard Juliana, Dara Junardy, Fransiska Devy Kamilah Da’inawari Khalissa Sekar Amanda Sudarjat Kilala Tilaar Kornelia Rosvita Manek Kunto Wibisono Kunto Wibisono Kunto Wibisono LAKSMI AMBARSARI latifah K Darusman Latifah K Darusman Latifah K Darusman Latifah K. Darusman Lelly Aulia Damarhati Lina Noviyanti Sutardi Lisnawati Tumanggor Liwanda, Novian Lucky Lia Faiza Luky Adrianto M, Kornelia Rosvita M. Agung Zaim Adzkiya M. Rafi Maily Mustofa MARIA BINTANG Maria Bintang Maria Magdalena Maulana, Asy Syifa Tickna Maulidya, Innes Mayang Anggita Cahya Kurnia Mayang Anggita Cahya Kurnia Milanda Fiorella Gultom Milanda Fiorella Gultom Mira Takene Mohamad Rafi Mono Rahardjo Muhamad Rizal Martua Damanik Muhamad Syukur Muhammad Maftuchin Sholeh Muhammad Musthofa Muhammad Riyo Agung Kurnia Mulyati, Teti Mustika Weni Mustofa, Maily Muthoharoh, Rini Najmah Najmah Najmah Najmah, Najmah Nella Angelina Simanjuntak Nelly Marliani Nevianti Zamany Ning Harmanto Norman Razief Azwar Novita Sari Nurul Khumaida Nurul Komala P.Roswiem, Anna Pebra Heriansyah Popi Asri Kurniatin Poppy F. Arifin Priyadi, Yogo Suro Puspa Julistia Puspita Putra, Raihan Permana Putri, Dita Audia Qomariyah, Eka Nurul Rachmi Ridho Rahmadansah Rahmadansah Raphael Aswin Susilowidodo Rara Annisaur Rosyidah Ratna Puspita Ratna Puspita, Ratna Rerenstradika Tizar Terryana Riki Riki Rikno Harmoko Rini Muthoharoh Rini Novita Riski Rostantinata Riti, Dominggus Ngongo Riyan Alifbi Putera Irsal Rizki Fitria Rizki Fitria Ros Sumarny Rosalina Wisastra Roswiem, Anna Priangani Rusmiyati, Henny Saat Egra Sadwika Najmi Kautsari Safira Khoirunnisa Safira Khoirunnisa Sandra Arifin Aziz Seliani Fitria Mukti Shobiroh Nuur' Alimah Sholeh, Muhammad Maftuchin Siahaan Rafael Siti Nur'aeni Sri Suhesti Sri Suhesti Sri Suhesti Sriwongo, Stephanie Subroto, Erna Sukma, Dewi Suryani Suryani Suryani Suryani Suryani Suryani Susilowidodo, Raphael Aswin Syamsul Falah Syarifah Iis Aisyah Takene, Mira Takeshi Katayama Taopik Ridwan Taufik Ridwan Taufik Ridwan Taufik Ridwan, Taufik Taureza, Aulia Tiara Rizky Utami Tilaar, Kilala Tony Sumaryada Toshisada Suzuki Tubagus Iqbal Maulana Tumanggor, Lisnawati Utami Dyah Safitri Weni, Mustika Wisastra, Rosalina Wulan Tri Wahyuni Wulansari, Laela Yogo Suro Priyadi Yoshua Shandy Yudha Yuliana Galih Dyan Anggraheni Yulianto Zamany, Nevianti