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PENDEKATAN STEWART DALAM pH DARAH YANG MENDASARI ASIDOSIS METABOLIK Efrida Efrida; Ida Parwati; Ike Sri Redjeki
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 2 (2013)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i2.1061

Abstract

Metabolic acidosis is the most frequent acid-base disorder in patients of the Intensive Care Unit. By conventional approach based onpH value, [HCO3–], and base deficit (BD) from blood gas analyzer (BGA) measurement are often inappropriate with the clinical stateand inadequate in explaining the mechanism of the metabolic acidosis. The Stewart approach states that the blood pH is determinedby a strong ion difference (SID), the carbon dioxide tension (pCO2), the total concentration of non-volatile weak acid. The Stewartapproach may give a better understanding of the mechanisms that underlie the metabolic acidosis. The purpose of this study is to knowthe correlation of blood pH value measurement from BGA and calculation based on Stewart approach and identifying the mechanismsthat underlie a metabolic acidosis. In this study an analytic observational cross-sectional method was used. The examined subjectsconsisted of 71 patients who were admitted with a metabolic acidosis at the ICU from July up to August 2007. All patients were measuredfor their blood pH, pCO2, [HCO3–], BD, sodium, potassium, calcium, magnesium, chloride, lactate, albumin, and phosphate. The resultwas reported as the mean and standard deviation. The data were analyzed by Pearson’s correlation test and linier multiple regression.Statistical significance was determined at p < 0.05. The mean values of blood pH measurement from BGA and blood pH calculationbased on the Stewart approach were 7.33 (0.11) and 7.49 (0.11) (r = 0.681; p < 0.001). Most patients had two underlying mechanisms ofmetabolic acidosis. Hyperlactatemia was present in 61.8%, hyperchloremia was present in 58.2% of patients. Based on this study so far,by using the Stewart approach there is an excellent and significant correlation between the blood pH measurement from BGA and bloodpH calculation. Hyperlactatemia and hyperchloremia are the main causes of the metabolic acidosis in patients of the ICU ward.
KEABSAHAN ENGRAILED-2 DI KANKER PROSTAT Elsa Yulius; Ida Parwati; Anna Tjandrawati; Dewi Kartika T
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1084

Abstract

Prostate cancer is one of the most common malignancy of males found worldwide including in Asia, and is the second most commoncause of male death in Western countries. Specific cause of prostate cancer is not yet elucidated, but there are several risk factors as well.The diagnosis of prostate cancer is confirmed by digital rectal examination (DRE), prostate-specific antigen (PSA), and a biopsy withtrans-rectal ultrasonography (TRUS). However, the sensitivity of DRE and PSA examinations are low and not good enough to detectprostate cancer. Currently there is a new test called Engrailed-2 (EN2) examination with enzyme-linked immunosorbent assay (ELISA)micro method. The test is simple and uninvasive one. This study is aimed to know the validity of urinary EN2 protein level measurementto detect prostate cancer by analysing. This study was conducted at Dr. Hasan Sadikin Hospital Bandung between November 2012 untilMarch 2013. This study was a diagnostic test with cross sectional design. The subjects of study were men whom suspected to have prostatecancer by DRE, PSA value (≥4 ng/mL) and TRUS biopsy. Urinary EN2 protein levels were measured using ELISA micro method. Statisticalanalysis used Mann-Whitney test, table 2×2 and ROC curve. In this study there are 50 subjects, where the result of positive biopsy forprostate cancer were found on 17 subjects and the negative were on 33 subjects. The study results on cut off value of >0.7 ng/mL gavethe sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of 94.12%, 63.64%, 57.1%, 95.5% and 74%,respectively. In conclusion of this study, that the urinary EN2 protein level examination using ELISA micro method has the high sensitivityand moderate specificity, thus these procedure primarily can be used for the screening of prostate cancer.
KENASABAN KADAR 8-HYDROXY-2-DEOXYGUANOSINE (8-OHDG) SERUM DENGAN DERAJAT DEFISIT NEUROLOGIS PADA STROK ISKEMIK {Correlation of Serum 8-Hydroxy-2-Deoxyguanosine (8-OHdG) With Neurological Deficits in Ischemic Stroke} Liza Liza; Ida Parwati; Andi Basuki Prima Birawa
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 1 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i1.1223

Abstract

The brain blood flow blockage in ischemic stroke increased oxidative stress and free radicals that cause neurotic cells damage.Computed Tomography Scanning (CT scan) assesses the brain structural damage but not the brain cells damage quantitatively. Adiscrepancy between CT scanand clinical symptoms of ischemic stroke patient is often found. 8-Hydroxy-2-Deoxyguanosine (8-OHdG)serum concentrationis the result offree radical interaction with the cluster C8guanine bases, was used to assess the degree ofneuron cellsdamage, oxidative stress levels. Increase of 8-OHdG serum concentration indicates brain cellsdamage as reflected in the neurologicaldeficitsby the The National Institutes of Health Stroke Scale (NIHSS). The aim of this study was to know the correlation of 8-OHdG serumconcentration with the determination of the degree of neurological deficitby NIHSS inischemic stroke patients. Seventy-two patients withacute ischemic stroke were enrolled in the Dr. Hasan Sadikin Hospital from August 2013 to January 2014. The research was carriedout by cross sectional study design. Statistical analysis was performed by Kruskal-Wallis test, rank Spearman’s correlation test. Mildneurological deficit with a median of 8-OHdG serum concentrations was 3.9 ng/mL (3.3−12.0 ng/mL), moderate neurological deficitwas 23 ng/mL (8.0−51.0 ng/mL) and the severe neurological deficit was 77.5 ng/mL (54.0−97.0 ng/mL). The correlation of 8-OHdGserum concentration with neurological deficits in acute ischemic stroke was 0.912 (p<0.001. Correlation of serum 8-OHdG concentrationwith neurological deficits in acute ischemic stroke was 0.912 (p<0.001). Based on this study result it can be concluded, that this researchhas a strong meaningful positive correlation of 8-OHdG serum concentration with neurological deficits as examined by NIHSS. Inconclusion, serum 8-OHdG has a strong meaningful positive correlation with neurological deficits. 8-OHdG serum concentration can beconsidered to be used to assess discrepancy between CT scan and clinical symptoms as well in health facilities without CT scan.
Laboratory Diagnostic Approach and Interpretation in Gram-Negative Bacterial Infection: A-Case Report of Sepsis in Secondary Hospital Settings Adhi Kristianto Sugianli; Ida Parwati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 3 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i3.1563

Abstract

Gram-Negative Bacteria (GNB) have been widely reported to cause worldwide infections and life-threatening. The high prevalence of drug-resistant GNB causes the treatment of GNB to become difficult. This case report describes a stepwise laboratory approach and interpretation for Gram-negative bacteria infection in sepsis patients. An 84-year-old female patient with a history of congestive heart failure, after three weeks of hospitalization, GNB was proven as the cause of sepsis. Laboratory approach for inflammation (C-reactive protein, procalcitonin) was made and confirmed with a positive culture of several specimens (sputum, urine, and blood). The identification of bacterial-culture revealed as Carbapenem-resistance Klebsiella pneumoniae and Extended-spectrum Beta-lactamases Escherichia coli. This case highlights GNB as a potential agent to worsen the infection (sepsis) and also a useful approach for the detection of multidrug-resistant bacteria, particularly in secondary hospital settings. The application and interpretation of integrated clinical and laboratory criteria may bring out better and effective patient management.
PENGANGKAAN (KUANTIFIKASI) PERIKSAAN PULASAN GRAM DI BERBAGAI JENIS BAHAN PEMERIKSAAN Adhi Kristianto Sugianli; Ida Parwati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1048

Abstract

In a clinical microbiology laboratory the Gram staining is used to classify bacteria on the basis of their forms, sizes, cellularmorphologies, and Gram reactions. Additionally it is a critical test for rapid presumptive diagnosis of infectious agents and serves toassess the quality of clinical specimens. Several methods of Gram staining quantification are already applied: Canadian Coalition forQuality in Laboratory Medicine (CCQLM), Clinical Microbiology Proficiency Testing (CMPT), and World Health Organization (WHO).Each method consists of several criteria for quantification and its interpretation, such as neutrophil cell (polymorphonuclear cells),squamous epithelial cell, and number of microorganisms. Those methods aren't limited in sputum specimen, but also could be used forother specimen such as urine, vaginal discharge, and other body fluids. These methods are also could be used as screening for specimenbefore it is continued into further testing. Even though there is several limitation for each method, quantification method of Gramstaining could be provide better diagnostic value in microbiology laboratory as an early detection in the examination to get betterdiagnosis as well as treatment.
HYBRIDIZATION-BASED NUCLEIC ACID AMPLIFICATION TEST TERHADAP CARTRIDGE-BASED NUCLEIC ACID AMPLIFICATION TEST TERKAIT MULTIDRUG-RESISTANT TUBERCULOSIS (Hybridization-Based Nucleic Acid Amplification Test towards Catridge-Based Nucleic Acid Amplification Test in Multidrug-Resistant Tuberculosis) Ivana Agnes Sulianto; Ida Parwati; Nina Tristina; Agnes Rengga I
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 3 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i3.1274

Abstract

Indonesia has high burden of multidrug-resistant tuberculosis (MDR-TB). Cartridge-based nucleic acid amplification test (CB-NAAT),which is recommended as a diagnostic method of MDR-TB by World Health Organization, is faster in achieving the result. This methoddetermines MDR-TB only from the rifampisin resistance, by detecting mutations that occur on the 81 bp hot-spot region of the rpoBgene. The isoniazid resistance is not included in the determination of MDR-TB by this method. Hybridization-based NAAT (HB-NAAT)detects MDR-TB not only from the rifampisin resistance (codon 526 and 531 rpoB gene), but also from the isoniazid resistance (codon315 katG gene). The aim of this study was to know the validity of the HB-NAAT in detecting MDR-TB using sputum with CB-NAATas the gold standard in a diagnostic study. All of 51 sputums were collected during June 2013 from patients suspected pulmonaryMDR-TB at Dr. Hasan Sadikin General Hospital. The result of CB-NAAT were 16 MDR-TB, 12 TB non MDR, and 23 non TB. HB-NAATexamination results were 3 MDR-TB, 25 TB non MDR (3 RMR, 6 IMR, 16 susceptible) and 23 non TB. The sensitivity of HB-NAAT was18.75% and specificity 100%. Low sensitivity values may due to the high mutation variations in the samples. So it could not be detectedonly by codons 526 and 531 for rifampisin resistance. For the detection of isoniazid resistance, HB-NAAT have optimal primer at lowconcentrations and it also need more than katG genes to detect isoniazid resistance. Based on this study, it can be conclued, that HBNAAThas low sensitivity but high specificity in the detecting MDR-TB.
FUSI GEN BREAKPOINT CLUSTER REGION ABELSON KINASE (BCRABL) DAN UJI HEMATOLOGIS RUTIN Delita Prihatni; Ida Parwati; Rahmat Sumantri; Rully MA. Roesli; Nurizzatun Nafsi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 1 (2013)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i1.440

Abstract

Chronic myeloid leukemia (CML) is a type of Chronic myeloproliferative disorders in pluripotencial stem cell haematopoiesis cell disease caused by somatic mutation chromosomal translocation of the Abelson (ABL) and Breakpoint Cluster Region (BCR) genes on chromosomes 9 and 22. The Breakpoint Cluster Region Abelson Kinase (BCR-ABL) gene encodes different fusion transcripts of messenger Ribo Nucleic acid (m RNA)/type of fusion gene that vary in size depending on the breakpoint in the BCR gene. The majority of CMLcases have been shown to have either b3a2 or b2a2 fusion gene. This research is a preliminary study designed to know how to identify a quantification BCR-ABL gene and expression fusion of gene and its relation to routine haematological parameters. The researchers analyzed 12 adults who were positive using a quantification ratio BCR-ABL and Glucose-6-Phosphate Dehydrogenase (G6PDH) as a house keeping gene by real-time quantitative polymerase chain reaction (RQ PCR) of chronic phase of CML patients, qualitative of translocation of BCR-ABL gene by gel agarose and routine haematological tests by a haematologic analyzer. The average quantification ratio of BCRABL gene and G6PDH was 0.0881, 50% patients had b3a2 fusion gene, 41.6% had b2a2 dan 0.4% had e1a1. Fusion gene b3a2 showed a quantification ratio, haemoglobin level and leukocyte count higher compared to b2a2 fusion gene.
RANCANGAN PRIMER SPESIFIK GEN MACROPHAGE MANNOSE RECEPTOR (MMR) UNTUK POLYMERASE CHAIN REACTION (PCR) DAN SEKUENSING DEOXYRIBO NUCLEIC ACID (DNA) Yani Triyani; Nurizzatun Nafsi; Lelly Yuniarti; Nanan Sekarwana; Endang Sutedja; Dida Ahmad Gurnida; Ida Parwati; Bachti Alisjahbana
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i2.1120

Abstract

The order (sequencing) determinationof DeoxyribonucleicAcid (DNA) bases is the gene’s most basic information, using the methodof Polymerase Chain Reaction (PCR) as its stage. A key factor of successful detection by PCR is specific PCR primer design choice. Thedetection of diversity of Mycobacterium Mannose Receptor (MMR) gene, responsible for recognizing mannosylated antigen structureof Mycobacterium tuberculosis (M.tb) by DNA sequencing of exon 7 chromosome 10p12, related to susceptiblity for PulmonaryTuberculosis(TB), was first performed in China in 2012. The purpose of this study was to find specific primerfromboth design originatedfrom the research in China/primer I and my own design/primer IIby using Primer3 software. This study was based on 10 healthy subjectsand was a preliminary study of a research titled. The Relationship of Single Nucleotide Polymorphisms (SNPs) of Macrophage MannoseReceptor Gene to Pulmonary Tuberculosis Cases. The examination materials consist of 3 mL of EDTA blood and DNA extraction from itsbuffy coat. The resulting DNA was processed by PCR to amplify MMR gene with primer I and II. The primer I successfully amplified DNAfragments up to 780bp while primer II only 329 bp. The MMR gene DNA sequencing analysis was performed on the amplification resultof both kinds primers by using DNA Baser and Ensembl−BLAST software. The results were different, DNA sequencing result by using theprimer I was found in several chromosomes and also in several loci. Whereas, by using the primer II, it was only found in chromosome10 and in the same locus. Based on this study, it can be concluded that the specific primer design is one of the most important factorsin the success of DNA sequencing.
MENINGITIS TUBERCULOSIS Silvia Rachmayati; Ida Parwati; A Rizal; D Oktavia
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 3 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i3.1166

Abstract

Tuberculosis meningitis is one of the clinical manifestations of extra-pulmonary TB, which is localized in the central nervous system(CNS). The mortality of these patients is usually caused by the delays in diagnosis and treatment; hence up to this day tuberculosismeningitis remains a health problem. The diagnosis of tuberculosis meningitis based on the discovery of M.tuberculosis established inthe cerebrospinal fluid (CSF) and is the gold standard by culture, although the examination results will be obtained after 6–8 weeks.Nevertheless the patient in this case needs an immediate and accurate treatment. Recently there is a rapid and accurate test to confirmthe diagnosis of tuberculosis meningitis, that is the detection of an immunoglobulin (Ig) M and G in the serum. The aim of this study isto know the validity and the correlation of IgG and IgM test to the gold standard by investigated them. The research is an observationalstudy conducted cross sectional in the Clinical Pathology Department of Hasan Sadikin Hospital, from October 2008 up to January 2009.Eighty samples were obtained, which included the criteria for patients with suspected tuberculosis meningitis. The result of this studyshows: sensitivity 94.1%, specificity 100%, positive predictive value 100%, negative predictive value 95.8%, positive infinite likelihoodratio (LHR), negative LHR 0.06 and kappa 0.95. According to this study it can be concluded, that the IgM/IgG TB is a test that has anexcellent validity and correlation for the future.
The Relationship of Genetic Diversity of Human Immunodeficiency Virus-1 with Acquired Drug Resistance Mutation in HIV Patients on First-Lines Antiretroviral Therapy Efrida Efrida; Andani Eka Putra; Ida Parwati; Jamsari Jamsari
Majalah Kedokteran Andalas Vol 45, No 3 (2022): Online July 2022
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v45.i3.p400-407.2022

Abstract

Aim: This study aims to determine the relationship of HIV-1 genetic diversity with acquired drug resistance mutation in HIV-1 patients who receive first-line antiretroviral drugs at RSUP Dr. M. Djamil Padang. Methods: The study was conducted on 20 people with HIV who were diagnosed based on HIV-1 antibody screening tests using simple / rapid methods at the Central Laboratory of RSUP Dr. _ _ M. Djamil Padang and had received first-line ARV therapy for at least 6 months . Furthermore, proviral HIV-1 DNA extraction was carried out and amplification using a PCR (nested PCR technique) with a specific primer against reverse transcriptase at the Biomolecular Laboratory of the Microbiology Section of the Faculty of Medicine, Andalas University .  Result: The results showed that HIV-1 genetic diversity and changes in ARV regimens with the discovery of drug resistance mutations obtained for first-line ARVs were expected to be the basis for the management of HIV/AIDS patients. Conclusion: The conclusion of this study is that HIV-1 genetic diversity found is a form of recombinant CRF-AE and CRF- BC and subtypes A and B , a acquired drug resistance found is M184L and T215N (types of mutations that are resistant to NRTI- class ARVs) 
Co-Authors - Hidayat, - - Liza A K Sugianli A Rizal Abdul Hadi Martakusumah Adhi K Sugianli Adhi Kristianto Sugianli Adrian Suhendra Agnes Rengga I Agnes Rengga Indrati Ahmad Faried Ahmad Rizal Ahmad Rizal Ganiem Al Rasyid Amanda Widayanti Andani Eka Putra Andi Basuki Prima Birawa Andi Basuki Prima Birawa Andre van der Venn Anggra Filani Anggraini Alam Ani Melani Maskoen Anis Karuniawati Anita Rahmawati Anna Tjandrawati Arto Yuwono Arto Yuwono Soeroto Asih Kurniasih Astrid Tamara Maajid Budiman Aziz, Aurizal Ahmad Bachti Alisjabana, Bachti Bachti Alisjahbana Bachti Alisyahbana Banundari Rachmawati Basti Andriyoko Basti Andriyoko Basti Andriyoko Bethy S. Hernowo Bethy Suryawati Hernowo BUDI SETIABUDIAWAN Budi Setiabudiawan Budiman , Budiman Christian Prijana, Christian Chrysanti Murad Chrysanti Murad, Chrysanti Cissy B Kartasasmita Cissy B. Kartasasmita Coriejati Rita D Oktavia Dahlan, Zulkarnain Delita Prihatni Dewi Kartika T Dewi Kartika Turbawaty Dewi Lokida Dewi Nurhayati Dick van Soolingen Dida Ahmad Gurnida Djatnika Setiabudi E. Risdiyani Edhyana Sahiratmadja Efrida Efrida Efriyani, Yuhpita Indah Elsa Yulius Endang Samaun Endang Sutedja Erica Catarina Erna Jupri Evan Susandi Fairuz Quzwain Febriana, Lutvi Fiona Lestari Fiona Lestari, Fiona Ganiem AR Gde Sindu Mega Hartanto Bayuaji Hendra Subroto, Hendra Herri S. Sastramihardja Herry Garna Hinta Meijerink I. Sjahid Idaningroem Sjahid Ike Sri Redjeki Indahwaty, - Indriani Silvia Intan Permatasari Ivana Agnes Sulianto J. E. Gunawan Jamsari Jamsari Johanes C. Mose Johanes C. Mose Julia Hartati Juniati Juniati Komariyah, Nining Syamsi Larissa Larissa Latifah, Armina Lelly Yuniarti Leni Lismayanti Lidya Chaidir Linda Choerunnisa Lisda Amalia Lismayanti , Leni Livia Noviani Liza Liza Luhung Budiailmiawan Marziah, Cut Mike Rezeki Nanan Sekarwana Nataprawira, Heda M Nawang Wulandari, Nawang Nenny Gustiani Nenny Gustiani Ni Sayu Dewi B. Nida Suraya Nida Suryana Nina Susana Dewi Nina Tristina Noormartany Nur Atiik Nurizzatun Nafsi Nurlina Sirait Prawoto Prawoto Prayudi Santoso Prihatni, Delita Pujiyanti, Hapsari Rachmat Soelaeman Rahmat Sumantri Ramdan Panigoro Reinout van Crevel Reinout van Crevel RINI SUNDARI RINI SUNDARI, RINI Rita Rachmayanti Rizki Andriyani Rubin Surachno Gondodiputro Rudi Wisaksana Rully Marsis Amirullah Roesli Ryan Bayusantika S. Nilawati Silanas, Ilman Silvia Rachmayati Soeroto, Arto Y Sofa Dewi Alfian Sofiati Dian Sukartini, Ninik Sylvia Rachmayati T. M. Sudiro Thatit Nurmawati, Thatit Tiene Rostini, Tiene Tjahjani M. Sudiro Towifah Fauziah Choerunisa Turbawati DK Uni Gamayani Uun Sumardi, Uun Wia Melia Wida Purbaningsih Wulan Ardhana Iswari Wulan Ardhana Iswari, Wulan Ardhana Yani Triyani Yani Triyani Yanti Yanti Yeva Rosana Yovita Hartantri Yusuf Sulaiman Effendi Zakiyah, Neily