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All Journal AGRARIS: Journal of Agribusiness and Rural Development Research JPM (Jurnal Pemberdayaan Masyarakat) Jurnal Analis Kesehatan Jurnal Kesehatan INTEK: Jurnal Penelitian Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Social, Humanities, and Educational Studies (SHEs): Conference Series Jurnal Prakarsa Paedagogia Jurnal Sasindo Unpam Medicra (Journal of Medical Laboratory Science/Technology) Journal of Strategic and Global Studies Mitra Raflesia (Journal of Health Science) Dharmas Education Journal (DE_Journal) Jurnal Abdimas Kartika Wijayakusuma Almufi Jurnal Pengabdian kepada Masyarakat Indonesian Journal of Innovation Studies PARADIGMA Tsaqofah: Jurnal Penelitian Guru Indonesia Arfak Chem: Chemistry Education Journal Jurnal Pengabdian Masyarakat Bangsa Bangun Desa : Jurnal Pengabdian Masyarakat Enrichment: Journal of Multidisciplinary Research and Development Jurnal Keamanan Nasional Jurnal Akademik Pengabdian Masyarakat Jurnal Kedokteran Meditek Jurnal Kajian Stratejik Ketahanan Nasional Journal of Communication and Public Relations (JCPR) Manajemen Pelayanan Kesehatan JUEBIR: Journal of Economics and Business Research Jurnal Legislasi Indonesia Indonesian Journal Health Science and Medicine House of Wisdom: Journal on Library and Information Sciences
Puspitasari Puspitasari
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Journal : Jurnal Kesehatan

 1 
Optimization of Complete Blood Count Results with Variations in Specimen Handling and Whole Blood Secondary Homogenization Techniques Aliviameita, Andika; Puspitasari, Puspitasari; Primastuti, Yuridistya Putri; Batis, Afifah
Jurnal Kesehatan Vol 15 No 2 (2024): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v15i2.4578

Abstract

Specimen homogenization and the addition of anticoagulants are pre-analytical steps that influence the accuracy of the test results. Complete blood count (CBC) is a screening test that supports disease diagnosis and aids in determining the appropriate therapy for patients. Inadequate specimens are inappropriate for subsequent testing, as they can lead to inaccurate results in the CBC examination. This study aims to determine the optimization of complete blood count results with variations in specimen handling and secondary homogenization techniques for whole blood. The type of research used is quantitative analysis using laboratory experimental methods. The study was conducted in May 2024 in the Clinical Pathology Laboratory of the Diploma-4 Medical Laboratory Technology Study Program, Faculty of Health Sciences, Muhammadiyah University of Sidoarjo, using 48 blood samples. The results of the MANOVA test indicate that the type of anticoagulant has a significant effect on the erythrocyte count (p=0.041) but does not have a significant impact on the leukocyte count (p=0.844) and platelet count (p=0.920). Meanwhile, the homogenization technique does not significantly affect the erythrocyte count (p=0.959), leukocyte count (p=0.991), or platelet count (p=0.867). This study concludes that the secondary homogenization technique has no significant effect. In contrast, the type of anticoagulant significantly impacts the results of the Complete Blood Count (CBC) test. This research suggests collecting whole blood specimens using Ethylene Diamine Tetra Acetate (EDTA) vacutainers as the anticoagulant and applying a secondary homogenization technique before performing the CBC analysis.
Optimization of Whole Blood Specimen Handling for Complete Blood Count (CBC) Results and Blood Cell Morphology Aliviameita, Andika; Puspitasari, Puspitasari; Amilya, Widya Intan; Savitra, Igfina El
Jurnal Kesehatan Vol 16 No 2 (2025): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v16i2.5126

Abstract

Hematology analyzers are used in almost all clinical laboratories for CBC testing. CBC test results are crucial, as they play a significant role in establishing diagnoses and determining the appropriate therapy for patients. This study aims to analyze the effect of anticoagulant type, storage temperature, and delay time on the results of erythrocyte, leukocyte, and thrombocyte counts in whole blood samples. The study design employed was a laboratory experiment with a total of 64 samples, which were subjected to a combination of treatments involving anticoagulant type (Vacutainer and conventional), storage temperature (room temperature and refrigerator), and delay time for testing (0, 6, 12, and 24 hours). The examination was conducted using a hematology analyzer, and data analysis was performed using the Kruskal–Wallis test and the post hoc Mann–Whitney U test. The results showed that there was no significant effect of the three factors on the number of erythrocytes and leukocytes. However, the delay time significantly affected the platelet count (p=0.004), with significant differences observed between 6 hours and 24 hours, and between 12 hours and 24 hours. The delay in blood examination with K3EDTA and Na2EDTA affects the morphology of erythrocytes and leukocytes. Abnormal changes occur after 6, 12, and 24 hours of storage. The gold standard is a fresh whole blood sample collected in a K3EDTA vacutainer tube with the correct volume and analyzed within a maximum of 2 hours at room temperature.
Co-Authors Abadi , Totok Wahyu Adhitya Marendra Kiloes, Adhitya Marendra Agus Salim Agustanto, Pulung Akbar, Aulia Rizky Fadhillah al-akbar, Wildan Amalia , Fara Putri Ambarani, Putri Yuli Ambarwati, Marfu'ah Amilya, Widya Intan Andika Aliviameita Anggi Anggraeni Kusumoningtyas Anita Anita Aprilianingsih, Sri Athi, Fina Umu Rif Bagus Kukuh Udiarto Batis, Afifah Chylen Setiyo Rini Dewi Yanti Dindadianti , Afif Ida Endang Wuryandini, Endang Evi Rinata Fatimatasari, Fatimatasari Gita Farista Haniah, Lutfi Atul Haryati Haryati Hasanah, Muslimah Uswatun Humesi, Lusi Ibnu Hamad Ilmi, Isyfa'ul Imamah, Siti Aliyatul Innabila, Syifa Intantri, Risma Iswari, Miranti Florencia Jamilatur Rohmah Karim, Erna Khomaroh, Annisatul Krisdianti, Rika Kuncoro, Sonni Kusumoningtyas, Anggi Anggraeni Maha, Maha Mardi Santoso Marwan Riki Ginanjar Masúd, Fajriyati Mas’ud B, Mas’ud B Maula, Nala Innayatul Melati Afriani Miftahul Mushlih MUFIDAH, LAILATUL Muhammad Syafei, Muhammad Mukhamad Najib Muttaqijn, M. Imam Nasiro, Siti Nastiti, Hasna Widya Ngurah Ayu Nyoman Murniati Nirosa, Givari Dwi Nisa, Destriya Alfiara Nuraisyah Nuraisyah Nuratiqah, Nuratiqah Nurfadhillah, Septy nurhuda, zamzam Primastuti, Yuridistya Putri Purwanti, Karunia Nurullita Putra, Ariza Bima Putri, Alifah Ananda R. Sapto Hendri Boedi Soesatyo Rahman, Rahmadani Ratna Wulandari Restiani, Restiani Rini, Chylen Setyo Risma Risma Riyandi, Ekwan Dwi Rizal Fauzi Rofi’i, Aziz Rohmanudin, Rohmanudin Romdhoni , Arif Runturambi, Arthur Josias Saidah, Sukma Nur Saputra , Muhammad Rizky Sariyadi, Sariyadi Savitra, Igfina El Sela Mitha Selviana Selviana Setiyo Rini, Chylen Silfia Silfia, Silfia Silitonga, Bentonius Situmeang, Boima Sriwijayanti, Sriwijayanti Sugiarti Sugiarti Sunarmi Sunarmi Suzanna Ndraha Syah, Muhamad Jawal Anwarudin Syarif Permana Salingkat Syauqillah, Muhammad Syugiarto Syugiarto Tika, Setia Usman, Munjimayansari H. Verdiana, Verdiana Wibowo, Febrian Wahyu Wicaksana, Ragil Satria Yanti, Dewi Yasa, Ecep Suwardani Yasmin, Rizka Afifah Yonika Yeninastiti, Reny Zamzam Nurhuda