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All Journal Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Jurnal Kedokteran Brawijaya Jurnal Ilmiah Kursor Indonesian Journal of Urology Berkala Ilmu Kesehatan Kulit dan Kelamin Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Cancer Majalah Obstetri dan Ginekologi Medical Laboratory Technology Journal Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Manajemen Kesehatan Yayasan RS.Dr. Soetomo Majalah Patologi Indonesia Prosiding Seminar Nasional Multidisiplin Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Majalah Biomorfologi (Biomorphology Journal) Indian Journal of Forensic Medicine & Toxicology INDONESIAN MIDWIFERY HEALTH AND SCIENCES JOURNAL Indonesia Journal of Physical Medicine and Rehabilitation Indonesia Journal Physical Medicine and Rehabilitation Cermin Dunia Kedokteran Indonesian Journal of Cancer The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Jurnal Pengabdian Masyarakat Bangsa Enrichment: Journal of Multidisciplinary Research and Development Jurnal Widya Medika

Willy Sandhika

Departemen/ SMF Patologi Anatomi , Fakultas Kedokteran Universitas Airlangga/ RSU Dr. Soetomo, Surabaya, Indonesia

Author-ID : 370004

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Ekspresi CD10 dan Bcl6 pada Limfoma Malignum Sel B Jenis Sel Besar Difus dan Hubungannya dengan Skor Indeks Prognostik Internasional Jimmy Hadi Widjaja; Willy Sandhika
Majalah Patologi Indonesia Vol 19 No 2 (2010): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.986 KB)

ABSTRAKLatar Belakang.Limfoma malignum sel B jenis sel besar difus adalah Limfoma malignum non-Hodgkinyang sering dijumpai. Penyakit ini memiliki gambaran klinis, morfologik, perubahangenetik, dan molekular yang heterogen. Sel limfoma dapat memiliki fenotip serupadengan sel sentrum germinativum normal ditandai dengan ekspresi CD10 dan Bcl6 yangdapat berhubungan dengan gejala klinis dan prognosis yang baik. Penelitian ini inginmenilai hubungan imunoekspresi CD10 dan Bcl6 dengan skor Indeks PrognostikInternasional (IPI).CaraPenelitian dilakukan secara retrospektif pada 22 sediaan kasus Limfoma malignum sel Bjenis sel besar difus dari Januari 2007 sampai Juni 2009 di Bagian Patologi AnatomikRumah Sakit Dr. Soetomo Surabaya. Semua bahan dilakukan penilaian ulang untukmenentukan diagnosis berdasarkan klasifikasi WHO 2001 berupa pulasan HE danimunohistokimia dengan CD10 dan Bcl6. Uji korelasi kontigensi digunakan untuk analisadata.HasilHasil penelitian ini menunjukkan hubungan yang bermakna antara ekspresi CD10 danekspresi Bcl6 dengan skor IPI (p< 0,05). Tidak terdapat hubungan bermakna antaraekspresi CD10 dengan Bcl6 (p > 0,05).KesimpulanEkspresi CD10 dan Bcl6 berkaitan dengan prediksi prognosis.

Role of CD44 and CD8 in Colorectal Adenocarcinoma metastatic Baiq Ratna Kumaladewi; Willy Sandhika; Heryawati Heryawati
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Colorectal adenocarcinoma is one of the most prevalent malignancies worldwide. The presence of regional lymph node metastases is an important prognostic indicator in colorectal adenocarcinoma. CD44 is a transmembrane protein and a cancer stem cell biomarker that plays a role in tumor proliferation, metastasis, and recurrence. The tumor microenvironment has a vital role in the stage of tumor development. CD8 is one of microenvironment's component which kills the tumor cells. This study aimed to analyze the expression of CD44 and CD8 and investigated their association with colorectal adenocarcinoma metastatic. This study's benefit is that the resulting study can be used as a prognostic factor in colorectal adenocarcinoma. This study included 56 paraffin blocks of patients diagnosed as colorectal adenocarcinoma during January 2017-December 2018 in Anatomic Pathology Laboratory Dr. Soetomo Hospital Surabaya. The paraffin blocks were sliced and stained with immunohistochemistry for CD44 and CD8. The parameters were a positive percentage of tumor cell and lymphocyte immunoreactivity against CD44 and CD8 antibodies. The percentage of CD44 is higher in the metastatic group, and the percentage of CD8 is higher in the non-metastatic group. There was a significant negative correlation between CD44 and CD8 expression in regional lymph nodes metastatic. These results indicated that CD44 and CD8 have a vital role in the incidence of metastasis.

The Cytotoxic Activity of Annona muricata Linn Leaves Ethanolic Extract (AMEE) on T47D Breast Cancer Cell Line Soilia Fertilita; Willy Sandhika; Desak Gede Agung Suprabawati
Medical Laboratory Technology Journal Vol. 6 No. 1 (2020): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Breast cancer is the most common cancer in women throughout the world, with new cases and deaths which continue to increase. Soursop leaves (Annona muricata L) have been used extensively in traditional medicine, including cancer. Acetogenin, alkaloids, and phenols contained in soursop leaves are known to have anti-cancer effects. Among them, acetogenin has the most dominant role and reported to have a cytotoxic effect on various cancer cell lines. This study aims to determine the cytotoxic activity of soursop leaf ethanol extract on T47D breast cancer cell line. Measurement of cytotoxic activity was carried out by the MTT method, and the viability percentage of T47D cells was calculated based on the absorbance values in the treatment, cell control, and media control groups of each replicate. The correlation between extract concentration and viability percentage of the T47D cell line was outlined in the regression equation to obtain the IC50 value. IC50 values of 109.91 ± 3.04 with R values 0.975 and R2 0.9508 obtained. R values close to 1 indicated a strong correlation between extract concentration and the percentage of living T47D cells. Meanwhile, the amount of R2 suggested that the level of AMEE had a 95.08% influence on the rate of cell viability, and the other 4.92% influenced by factors other than the AMEE dose. These results indicated that the ethanol extract of soursop leaves has a cytotoxic effect and has the potential to inhibit T47D cell proliferation in vitro.

Antimalarial activity of goat bile against Plasmodium berghei ANKA infection in BALB/c mice Kholida Nur Aini; Windya Tri Hapsari; Kartika Arum Wardani; Heny Arwati; Willy Sandhika
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 4, No 2 (2020)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v4i2.3540

Abstract Goat bile has been used by some Indonesian people to treat malaria and increase their stamina. This study aimed to prove whether goat bile toxic or not in BALB/c mice and to verify the antimalarial activity of goat bile at various concentrations in mice infected with Plasmodium berghei ANKA. Acute toxicity test was performed using twenty male BALB/c mice with an average body weight of 25 grams, which were divided into four groups. Mice were given 25%, 50%, and 100% goat bile, respectively, while negative control was given distilled water. Any change in weight, odor, color, agitation, appearance, color of urine and feces, coma, and death, were recorded. A different set of mice were infected with P. berghei ANKA. This study conducted using the posttest only control group design with four treatments and five replications. A four day-treatment of goat bile was given by oral gavage to find out its effect on parasitemia level. Infected mice were divided randomly into 4 groups, where the GBNeg group as negative control was given only distilled water. The GB25, GB50, and GB100 groups were treated with 25%, 50%, and 100% goat bile, respectively. The parasitemia was observed daily on Giemsa-stained tail blood smears of each mice. No death or other sign of toxicity was found in goat bile-treated mice. Goat bile showed anti-malarial activity. The parasitemia in all goat bile treated groups was lower compared with the negative control group. The ED50 of goat bile against the growth of parasite was 48,55 %. Goat bile is a potential source of new antimalarial therapies. Further investigations are recommended to yield new anti-malarial drug candidates. Keywords : Goat bile, parasitemia, Plasmodium berghei ANKA, ED50Correspondence : heny-a@fk.unair.ac.id

Sequestration of erythrocytes infected with Plasmodium berghei ANKA in BALB/c mice treated with goat bile Kartika Arum Wardani; Kholida Nur Aini; Heny Arwati; Willy Sandhika
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 4, No 2 (2020)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v4i2.3539

Abstract Sequestration of Plasmodium berghei ANKA-infected erythrocytes occurs in BALB/c mice as characteristic of Plasmodium falciparum infection in humans. Animals’ bile has been widely used for centuries in Traditional Chinese Medicine. Goat bile has been used in healing infectious and non-infectious diseases; however, no report on the use of goat bile against malaria infection and sequestration. The purpose of this study was to analyze the correlation between parasitemia and sequestration in the liver of P.berghei ANKA-infected BALB/c mice treated with goat bile. This research was an in vivo experimental study using the post-test control group design. The male BALB/c mice aged ± 6 weeks, body weight 20-25 g were used. The mice were divided into five groups where Group 1-3 were mice treated with goat bile 25%, 50%, and 100%, respectively. Group 4-5 were negative (sterile water) and positive controls (DHP). Parasitemia was observed daily from each mouse and the number of sequestered infected erythrocytes on the endothelium of sinusoids. The data were analyzed using t independent test. Antimalarial activity of goat bile was shown by the lower parasitemia in goat bile-treated mice compared with the negative control. The average number of sequestration was goat bile concentration-dependent manner. The higher the concentration, the lower the number of sequestration. Sequestration was correlated with parasitemia (p=0,0001). Sequestration of P.berghei ANKA-infected erythrocytes correlated with parasitemia, and was goat bile concentration-dependent manner. Keywords: Malaria, parasitemia, sequestration, goat bileCorrespondence: arwatiheny@gmail.com

Perbedaan Ekspresi Caspase 3 pada Limpa Tikus Model Sepsis yang Diinfeksi Escherichia coli ESBL dan dengan yang Diinfeksi Klebsiella pneumoniae Carbapenemase Lisa Savitri; Willy Sandhika; Agung Dwi Wahyu Widodo
Prosiding Seminar Nasional Multidisiplin Vol 2 No 1 (2019): Volume 2 No 1 Tahun 2019 (On Proses)
Publisher : KH. A. Wahab Hasbullah University

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Sepsis adalah suatu sindroma klinik yang terjadi karena adanya respon tubuh yang berlebihan terhadap rangsangan produk mikroorganisme. Pasien sepsis dengan infeksi bakteri penghasil ESBL 57,4% terinfeksi E. coli, 21,35% terinfeksi Enterobacter sp, dan 21,3% terinfeksi Klebsiella sp. Sepsis dapat diperberat oleh peningkatan kuman yang multiresisten terhadap bermacam antibiotik, seperti E. coli ESBL dan K. pneumoniae carbapenemase (KPC). Selama infeksi bakteri, faktor virulen diproduksi dan disekresikan dari patogen dan memicu sinyal apoptosis. Penelitian mengenai caspase 3 dianggap penting, karena caspase 3 merupakan efektor caspase terpenting yang bertanggungjawab atas morfologi dan perubahan biologis yang terlihat pada sel apoptosis. Jenis penelitian ini adalah true experimental dengan rancangan penelitian post test only control group design. Hasil penelitian menunjukkan bahwa ekspresi caspase 3 pada limpa mencit yang diinfeksi KPC adalah 65,25±12,69%, sedangkan yang diinfeksi E. coli ESBL adalah 33,75±3,862%, hal tersebut diduga karena adanya kapsul polisakarida yang mengelilingi KPC dan melindungi dirinya terhadap aksi fagositosis dan bakterisidal serum yang dapat dianggap sebagai penentu virulensi paling penting dari KPC dan menyebabkan mitokondria melepaskan ROS. Infeksi bakteri tersebut menyebabkan mitokondria memproduksi ROS dan memicu pelepasan sitokrom c. Sitokrom c akan memicu caspase 9 untuk berikatan dengan efektor caspase 3, sehingga terjadi apoptosis Kata kunci: Sepsis, ekspresi caspase 3, limpa tikus, Escherichia coli ESBL, Klebsiella pneumoniae carbapenemase

Use of bee honey as alternative medicine in protein energy deficiency R. Heru Prasetyo; Willy Sandhika; Djoni Susanto
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (519.323 KB)

The protein energy deficiency cause intestinal villus atrophy and epithel mucous damage. The effect of bee honey on histostructure of intestine was studied in the experimental mice as model of proteinenergy deficiency. The use bee honey in protein-energy deficiency shown to improve intestinal villus atrophy and epithel damage. In conclusion that bee honey can use as alternative medicine in protein energydeficiency

The difference of PGE2 and COX-2 expressions in various histological grading of endometrial endometrioid carcinoma Yusfita Evi Rosdiana; Willy Sandhika
Majalah Obstetri dan Ginekologi Vol. 25 No. 1 (2017): April
Publisher : Universitas Airlangga

Objectives: to analyze the expression of PGE2 and COX-2 in various histological grade of endometrial endometrioid carcinoma.Materials and Methods: Analytical observational study was performed on parafin block archives of endometrial endometrioid carcinoma with histological grade 1,2 and 3 in Anatomic Pathology laboratory Dr. Soetomo hospital. PGE2 and COX-2 expression were examined by immunohistochemical staining using PGE2 and COX-2 antibodies. The results were analyzed statistically.Results: There were increase of PGE2 expressions of in endometrioid carcinoma grade 2 compare to grade 1 and grade 3 compare to grade 2. The COX-2 expression was increase in histologic grade 3 compare to grade 1.Conclusion: The expression of PGE2 and COX-2 in endometrial endometrioid carcinoma were increased in higher histologic grade compare to lower histologic grade.

Association of age at menarche, parity, and hormonal contraceptive use with the histologic type of ovarian cancer Firda Azizah; Pungky Mulawardhana; Willy Sandhika
Majalah Obstetri dan Ginekologi Vol. 29 No. 3 (2021): December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V29I32021.118-123

HIGHLIGHT1. Relationship between age at menarche, parity, and contraceptive use with histologic type of ovarian cancer was analyzed. 2. A number of patients with ovarian cancer were analytically observed with retrospective cross-sectional approach and the histologic types of the cancer were determined.3. Age of menarche, parity, and hormonal contraceptive use was found not to have significant correlation with histologic type of ovarian cancer. ABSTRACTObjectives: This study analyzed the relationship between age at menarche, parity, and contraceptive use with histologic type of ovarian cancer.Materials and Methods: This study used an observational analytic with a retrospective cross-sectional approach. The research samples were 128 patients with ovarian cancer at Dr. Saiful Anwar Hospital, Malang, Indonesia, in 2017-2019. All patients underwent primary staging laparotomy. The histologic types of the ovarian cancer consisted of serous type as many as 45, mucinous 45, endometrioid 10, clear cell 20, and others 4. Data analysis used chi-square test.Results: The p value for the relationship between age of menarche and histologic type of ovarian cancer was p=0.500 (p> 0.05), parity p=0.313, and contraceptive use p=0.824. The distribution of clear cell was more common in multiparous, 40% of endometrioid found in nulliparous, while serous were more common in women with hormonal contraceptive use >5 years, whereas mucinous were more common in those with history of use of <5 years.Conclusion: There was no significant relationship between age of menarche, parity, and hormonal contraceptive use on histologic type of ovarian cancer.

Gambaran Histopatologi Nekrobiosis Lipoidika Maylita Sari; Trisniartami Setyaningrum; Willy Sandhika
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 26 No. 1 (2014): BIKKK APRIL 2014
Publisher : Faculty of Medicine, Universitas Airlangga

Latar belakang: Nekrobiosis Lipoidika (NL) merupakan keadaan dermatologis idiopatik yang menjadi masalah secara kosmetik dan berhubungan erat dengan penyakit diabetes mellitus. Pemahaman tentang patogenesis dan diagnosis NL sangat penting karena adanya komplikasi yang bisa terjadi seperti ulserasi dan karsinoma sel skuamosa, disamping kesulitan penegakan diagnosis NL melalui histopatologi. Tujuan: Memahami patofisiologi, gambaran klinis, dan histopatologi NL serta diagnosis banding granuloma non infeksi. Telaah kepustakaan: Perubahan vaskular dan degenerasi kolagen serta faktor lain adanya Antibody Mediated Vasculitis diduga terjadi pada patogenesis NL. Prinsipnya didapatkan kerusakan mikrosirkulasi pada individu non diabetik dengan NL. Pemeriksaan histopatologi menunjukkan terdapat granuloma interstitial dan palisade yang melibatkan jaringan subkutan dan dermis. Granuloma tersusun berlapis-lapis (tierlike, layered) dan bercampur dengan area degenerasi kolagen, terdiri dari histiosit, beberapa diantaranya limfosit berinti banyak, sel plasma, dan eosinofil. Penebalan dinding pembuluh darah dan pembengkakan sel endotel pada dermis bagian tengah sampai dalam. Imunofluoresens direk didapatkan immunoglobulin M, Ig A, C3, dan fibrinogen pada pembuluh darah. Manifestasi klinis ditandai dengan plak berbatas jelas dengan area atrofi kekuningan pada tengahnya dan tepi menonjol berwarna merah sampai ungu, yang bisa mengalami ulserasi, bilateral, dan multipel. kesimpulan: NL merupakan suatu penyakit degeneratif kronis pada jaringan konektif dermis, dengan etiologi yang tidak diketahui dan terjadi hampir seluruhnya pada penderita diabetes mellitus. Diagnosis ditegakkan secara klinis dan histopatologi. Gambaran histologi NL hampir sama dengan granuloma annulare, namun manifestasi klinis berbeda.Kata kunci: nekrobiosis lipoidika, diabetes melitus, histopatologi, granuloma.

Co-Authors A.A. Ketut Agung Cahyawan W Afif Nurul Hidayati, Afif Nurul Agung Dwi Wahyu Widodo Agustin Nurliani Aisha Grayli Cahyani Alief Yudo Astuti Alphania Rahniayu Anny Setijo Rahaju, Anny Setijo Ariani, Grace Aries Sasongko Astindari Astindari Astindari Astindari, Astindari Ayu Tyasmara Bagus Haryo Kusumaputra, Bagus Haryo Baiq Ratna Kumaladewi Bernadetta Jonan Betty Agustina Tambunan Brama Rachmantyo Budiono Budipramana, Vicky Sumarki Cita Rosita Sigit Prakoeswa Densy Violina Harnanti Dian Eskaningrum Djoni Susanto Dwi Murtiastutik DYAH FAUZIAH, DYAH Endi Prilansa Mahadi Erlina Erlina Faizah, Zakiyatul Fanny Gunawan Fertilita, Soilia Firda Azizah Gatut Hardianto, Gatut Gilda Hartecia Gondo Mastutik Grace Ariani Hartanto, Felix Hartecia, Gilda Heny Arwati Heriyawati, Heriyawati Heryawati Heryawati I Wayan Yudiana Ibrahim, Azarya Ida Septika Wulansari Ilmiah, Khafidhotul Imam Susilo INDROPO AGUSNI Irene Lingkan Parengkuan Irmadita Citrashanty, Irmadita Isnin Anang Marhana Iswinarno Doso Saputro iwan Syarif Jimmy Hadi Widjaja Jonan, Bernadetta Junita Jeanne Paliman Kartika Arum Wardani Kholida Nur Aini Kiki Apnita Sari Kintan Putri KUSUMASTUTI, ETTY HARY LaleMaulin Prihatina Linda Astari, Linda M. Yulianto Listiawan Mappamasing, Hasnikmah Marina Rimadhani Marina Rimadhani Martha Kurnia Kusumawardani Maylita Sari Maylita Sari, Maylita Meita Ardini Pratamasari, Meita Ardini Muhammad Miftahussurur Nanda Amalia Ramadhanti Navisa, Claudia Clary Nidya Ulfana Tsania Nila Kurniasari Novia Indriyani Adisty Nurina Hasanatuludhhiyah Nurul Wiqoyah, Nurul Poernomo, Adinda Sandya PUNGKY MULAWARDHANA, PUNGKY Purwati Purwati Purwati Puteri, Agnes Ilene Suprapto Qonitatillah, Ana R. Heru Prasetyo R.A. Astrid Putri Wandhita Rahel Yuana Sadikim Ratna Darjanti Haryadi Regina Martina Cilik Ridholia, Ridholia Rizaldi, Fikri Rulisiana Widodo Rusdamayanti Rusdamayanti Rwahita Satyawati Dharmanta Ryski Meilia Novarina Ryski Meilia Novarina, Ryski Meilia S.Pd. M Kes I Ketut Sudiana . Sabilal Alif Sari, Aditya Sita Sari, Festi Artika savitri, Lisa Sawitri Sawitri Sawitri Sawitri Sawitri Soilia Fertilita Sunarso Suyoso Sunaryo Hardjowijoto Suprabawati, Desak Gede Agung Tessy Badriyah, Tessy Theresia Fifi Judikristiani Tri Hartini Yuliawati Trisniartami Setyaningrum Tyasmara, Ayu Umi Choviva, Farida Ummi Maimunah Vinna Chrisdianti Widati Fatmaningrum Wilda Fitria Rachmadina Windya Tri Hapsari Winona May Hendrata Wiratama, Priangga Adi yasmin, dzakiyah Yusfita Evi Rosdiana

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