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All Journal Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Jurnal Kedokteran Brawijaya Jurnal Ilmiah Kursor Indonesian Journal of Urology Berkala Ilmu Kesehatan Kulit dan Kelamin Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Cancer Majalah Obstetri dan Ginekologi Medical Laboratory Technology Journal Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Manajemen Kesehatan Yayasan RS.Dr. Soetomo Majalah Patologi Indonesia Prosiding Seminar Nasional Multidisiplin Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Majalah Biomorfologi (Biomorphology Journal) Indian Journal of Forensic Medicine & Toxicology INDONESIAN MIDWIFERY HEALTH AND SCIENCES JOURNAL Indonesia Journal of Physical Medicine and Rehabilitation Indonesia Journal Physical Medicine and Rehabilitation Cermin Dunia Kedokteran Indonesian Journal of Cancer The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy Jurnal Pengabdian Masyarakat Bangsa Enrichment: Journal of Multidisciplinary Research and Development Jurnal Widya Medika

Willy Sandhika

Departemen/ SMF Patologi Anatomi , Fakultas Kedokteran Universitas Airlangga/ RSU Dr. Soetomo, Surabaya, Indonesia

Author-ID : 370004

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Perbedaan Dermatitis Seboroik dan Psoriasis Vulgaris Berdasarkan Manifestasi Klinis dan Histopatologi Astindari Astindari; Sawitri Sawitri; Willy Sandhika
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 26 No. 1 (2014): BIKKK APRIL 2014
Publisher : Faculty of Medicine, Universitas Airlangga

Latar belakang: Dermatitis seboroik (DS) dan psoriasis sering sulit dibedakan baik secara klinis maupun secara histopatologi. Anamnesis yang tepat dengan memperhatikan usia, riwayat keluarga, dan pemeriksaan klinis yang teliti serta ditunjang dengan pemeriksaan histopatologi, dapat menentukan diagnosis yang tepat. Tujuan: Mengevaluasi perbedaan DS dan psoriasis supaya klinisi dan patolog bisa membuat diagnosis yang benar.Telaah kepustakaan: Secara epidemiologi, terdapat berbagai perbedaan antara DS dan psoriasis. Hal itu bisa dilihat dari usia saat timbulnya lesi, jenis kelamin, ras, maupun genetik. Lokasi lesi dan manifestasi klinis juga mempunyai ciri yang berbeda. Biopsi kulit dibutuhkan untuk membantu menegakkan diagnosis yang tepat.Gambaran histopatologi DS bervariasi sesuai dengan perjalanan penyakitnya: akut, sub-akut, dan kronis, sedangkan psoriasis mempunyai ciri khas berupa pemanjangan rete ridges, abses Munro atau adanya abses Kojog. Simpulan: Terdapat beberapa perbedaan antara DS dan psoriasis dari usia pertama kali muncul lesi, lokasi lesi, manifestasi klinis dan gambaran histopatologi.Kata kunci: dermatitis seboroik, psoriasis, manifestasi klinis, gambaran histopatologi.

Kaposi’s Sarcoma-associated Herpesvirus Novia Indriyani Adisty; Sawitri Sawitri; Willy Sandhika
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 28 No. 1 (2016): APRIL
Publisher : Faculty of Medicine, Universitas Airlangga

Background: Kaposi’s sarcoma (KS) revealed by Moritz Kaposi first time in 1872. In the early 1980, the prevalence of SK began to increase dramatically. A new finding leads to growth, isolation, and characterization of a novel human herpes virus, now known as Kaposi’s sarcoma-associated herpes virus (KSHV) or Human herpes virus type 8 (HHV-8) from SK lesions. Puspose: To determine the role of herpes viruses in the pathogenesis of KS. Review: KS is a cancer that develops from cells lining the lymph vessels or blood vessels. A number of KSHV encoded protein is known to have the ability to oncogenic transformation, included are latent and lytic proteins. Latent proteins tend to increase survival and proliferation of infected cells, whereas lytic viral proteins are believed to support the growth factors and angiogenic paracrine secretion useful for tumor growth and progression. Conclusion: HHV-8 is necessary, but not sufficient to cause the KS, and other factors such as immunosuppression also play a role.Key words: Kaposi’s sarcoma, herpes viruses.

Melanocyte Function and Count of Leukotrichia in Vitiligo Using S100 Immunohistochemistry and Microphtalmia Associated Transcription Factor (MITF) M. Yulianto Listiawan; Marina Rimadhani; Willy Sandhika
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 28 No. 3 (2016): DESEMBER
Publisher : Faculty of Medicine, Universitas Airlangga

Background: Melanocyte of the hair follicle is one of the major sources of repigmentation in vitiligo. Leukotrichia is complete depigmentation with significant bleaching hair. Leukotrichia in nonsegmental vitiligo may contribute to the lack of response to medical treatment. Leukotrichia is often associated with absent of melanocyte, showing poor prognosis for vitiligo treatment. Purpose: To evaluate melanocyte count and function of leukotrichia’s melanocyte in vitiligo and comparing with other feature. Method: Melanocyte count in each feature of lesional skin in 18 segmental vitiligo patients were evaluated based on vitiligo extent tensity index (VETI). Melanocyte count has been evaluated using immunohistochemistry S100 and microphtalmia associated transcription factor (MITF). Results: Eighteen patients were nonsegmental vitiligo, and five of them had leukotrichia. Two of five leukotrichia patients did not express MITF nor melanocyte. There were no differences of melanocyte and MITF expression between patient with or without leukotrichia. Conclusion: Leukotrichia is a poor indicator for treatment response in vitiligo, but this research showed that leucotrichia may not contribute to the lack of response upon medical treatment.

Pengaruh Pemberian Ekstrak Jahe Merah (Zingiber officinale var. rubrum) terhadap Jumlah Sel Makrofag dan Pembuluh Darah pada Luka Bersih Mencit (Mus musculus) Jantan (Penelitian Eksperimental pada Hewan Coba) Rahel Yuana Sadikim; Willy Sandhika; Iswinarno Doso Saputro
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 30 No. 2 (2018): AGUSTUS
Publisher : Faculty of Medicine, Universitas Airlangga

Latar Belakang: Jahe merah (Zingiber officinale var. rubrum) telah digunakan sebagai obat tradisional khususnya menyangkut penyembuhan luka karena kandungan oleoresin dan minyak atsiri yang tinggi. Namun penjelasan secara ilmiah masih belum banyak diteliti. Proses penyembuhan luka sangat dipengaruhi oleh jumlah makrofag dan pembuluh darah, sehingga peneliti tertarik untuk mengetahui pengaruh pemberian ekstrak jahe merah terhadap jumlah sel makrofag dan pembuluh darah pada luka. Tujuan: Membuktikan bahwa ekstrak jahe merah dapat menurunkan jumlah makrofag dan meningkatkan jumlah pembuluh darah pada luka bersih mencit jantan. Metode: Penelitian analitik eksperimental dengan the post test only control group design. Luka bersih pada 32 subjek dibagi dalam 4 kelompok. Kelompok pertama diberi konsumsi aquades steril secara oral selama 3 hari dan kelompok kedua selama 5 hari. Kelompok ketiga diberi ekstrak jahe merah (50 mg/kg bb) secara oral selama 3 hari dan kelompok keempat selama 5 hari. Preparat jaringan kulit dibuat menjadi slide histologi. Slide diamati dengan mikroskop pembesaran 400x dan graticulae. Hasil penghitungan makrofag dan pembuluh darah dibandingkan dengan uji t-2 sampel bebas. Hasil: Jumlah makrofag kelompok perlakuan lebih sedikit dibanding kontrol pada hari ke-3 (p=0,008) namun tidak signifikan pada hari ke-5 (p=0,409). Jumlah pembuluh darah kelompok perlakuan dibanding kontrol tidak signifikan pada hari ke-3 (p=0,721) dan ke-5 (p=0,365). Simpulan: Ekstrak etanol jahe merah dapat menurunkan jumlah sel makrofag pada hari ke-3 secara signifikan tetapi jumlah pembuluh darah tidak berbeda secara bermakna.

Perbandingan Terapi Kombinasi Laser CO2-Injeksi Triamsinolon dengan Injeksi Triamsinolon Monoterapi pada Keloid Brama Rachmantyo; M. Yulianto Listiawan; Dwi Murtiastutik; Willy Sandhika
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 30 No. 2 (2018): AGUSTUS
Publisher : Faculty of Medicine, Universitas Airlangga

Latar Belakang: Keloid adalah hiperplasia jinak dari jaringan fibrosa kulit. Gambaran histopatologi menunjukkan fibroblas dan serat kolagen yang berlebih. Prevalensi pasien keloid di Poli Kulit dan Kelamin RSUD Dr. Soetomo Surabaya adalah 1,4% (tahun 2013), 1,6% (tahun 2014), dan 1,5% (tahun 2015). Angka kekambuhan keloid pascaterapi injeksi triamsinolon mencapai 33% dalam 1 tahun. Laser CO2 dengan mode kontinu yang diikuti dengan injeksi triamsinolon memiliki efektifitas yang lebih baik dibanding injeksi triamsinolon monoterapi, dengan angka kekambuhan 15,4%. Laser CO2 mode fraksional memiliki masa penyembuhan lebih cepat daripada mode kontinu, karena ablasi terbatas hanya pada microscopic treatment zone (MTZ). Terapi kombinasi laser CO2 fraksional dan injeksi triamsinolon memadukan efek fototermolisis selektif dan efek antimitotik. Tujuan: Mengetahui efektifitas terapi kombinasi laser CO2 fraksional dan injeksi triamsinolon terhadap pasien keloid. Metode: Penelitian ini merupakan uji klinis terbuka yang membandingkan terapi kombinasi laser CO2 fraksional dan triamsinolon asetonid intralesi (perlakuan) dengan terapi tunggal triamsinolon intralesi (kontrol) pada pasien keloid. Hasil: Penelitian ini melibatkan 26 pasien keloid, 13 pasien kelompok kontrol dan 13 pasien kelompok perlakuan. Penurunan tinggi keloid yang signifikan terjadi pada kelompok kontrol dan perlakuan (p=0,005 dan p=0,000), tetapi selisih penurunan tinggi keloid antara kedua kelompok tidak signifikan (p=0,598). Penurunan kepadatan fibroblas pada kelompok kontrol terjadi secara signifikan (p=0,016), tetapi pada kelompok perlakuan meningkat tidak signifikan (p=0,958). Peningkatan kepadatan fibroblas dapat dikarenakan penyusutan kolagen, sehingga fibroblas tampak semakin padat. Simpulan: Terapi injeksi triamsinolon asetonid yang dikombinasi dengan laser CO2 fraksional belum memberikan dampak yang lebih baik daripada terapi tunggal injeksi triamsinolon.

Evaluasi Penggunaan Terapi Topikal Tretinoin 0,1% pada Striae Albae Densy Violina Harnanti; M Yulianto Listiawan; Linda Astari; Willy Sandhika
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 31 No. 2 (2019): AGUSTUS
Publisher : Faculty of Medicine, Universitas Airlangga

Latar belakang: Striae distensae (SD) adalah jaringan parut linier pada epidermis dan dermis akibat peregangan kulit yang melebihi batas elastisitasnya. Striae albae (SA) ditandai dengan garis hipopigmentasi dan terjadinya atrofi pada epidermis dan dermis. Hal tersebut dapat mengganggu fungsi sawar kulit bahkan gangguan transepidermal water loss (TEWL). Tujuan: Mengevaluasi perubahan klinis dan luas kolagen pasien SA sebelum dan setelah terapi tunggal krim tretinoin 0,1% selama 3 bulan. Metode: Penelitian eksperimental analitik yang membandingkan perubahan klinis dan persentase luas kolagen pasien SA sebelum dan setelah terapi tunggal krim tretinoin 0,1% selama 3 bulan di Unit Rawat Jalan (URJ) Kesehatan Kulit dan Kelamin Divisi Kosmetik dan Tumor Bedah Kulit Rumah Sakit Umum Daerah (RSUD) Dr. Soetomo Surabaya. Hasil: Panjang lesi SA sebelum dan setelah terapi krim tretinoin 0,1% tidak didapatkan perbedaan bermakna (p=0,341), begitu pula lebar lesi SA sebelum dan setelah terapi juga tidak didapatkan perbedaan yang bermakna (p=0,341). Persentase luas kolagen sebelum dan setelah terapi krim tretinoin 0,1% didapatkan perbedaan yang bermakna (p=0,0001). Visual Analog Scale (VAS) Improvement grade dengan skala 2 didapatkan pada 10 (90,9%) sampel penelitian sedangkan skala 3 didapatkan pada 1 (9,1%) sampel penelitian. Hasil penilaian VAS patient satisfaction grade didapatkan 10 (90,9%) sampel penelitian memberikan skala 6 dan 1 (9,1%) memberikan skala 7. Simpulan: Penggunaan krim tretinoin 0,1% pada SA selama 3 bulan tidak menunjukkan perubahan klinis yang bermakna, tetapi dapat meningkatkan persentase luas kolagen secara bermakna.

Effectiveness of Black Honey as an Antiinflammatory Substance in Rat’s Wound Infected by Staphylococcus aureus Fanny Gunawan; Willy Sandhika; Nurul Wiqoyah
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 33 No. 1 (2021): APRIL
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikk.V33.1.2021.13-18

Background: Black honey contains higher flavonoids, phenols, and minerals, which serves as antiinflammatory agents and promote faster wound healing. Purpose: This study aimed to determine the effectiveness of black honey on macrophage cells and blood vessels in rats’ wounds infected with Staphylococcus aureus. Methods: This was an experimental study with a post-test only control group design. This study involved 24 rats divided into 4 groups. The treatment was carried out for 5 days. The rats in the K1 group were wounded (cut) and given aquadest. The rats in the K2 group were wounded (cut), infected with Staphylococcus aureus, and given aquadest. The rats in the P1 group were wounded (cut), infected with Staphylococcus aureus, and treated with 2ml of black honey topically. The rats in the P2 group were wounded (cut), infected with Staphylococcus aureus, and treated with2ml of black honey orally. Observations were made histopathologicaly using hematoxylin-eosin (HE) staining by calculating the number of macrophage and blood vessels, and further using One-Way ANOVA. Result: The number of macrophages and blood vessels between groups was significantly different. The K2 group had the highest mean number of macrophage cells and blood vessels, and the K1 group was the least. The mean number of macrophage cells in the P1 group was more than the K1 group and less than the K2 and P2 groups, both significantly. Conclusion: Black honey was effective in reducing inflammation in wounds infected by Staphylococcus aureus.

The Effect of Snakehead Fish (Channa striata) Extract on Inflammation Reaction of Skin Wound Tissue in Rattus novergicus Wistar Strain Nanda Amalia Ramadhanti; Willy Sandhika; Agung Dwi Wahyu Widodo
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 33 No. 1 (2021): APRIL
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikk.V33.1.2021.48-54

Background: Snakehead fish has been associated with wound healing due to its high albumin content. Albumin can accelerate the inflammatory process so that tissue repair will be faster. Lack of albumin protein causes a person tend to experience prolonged wound healing. Purpose: This study aims to prove the effect of snakehead fish extract on the number of macrophages and blood vessels on the inflammatory reaction of skin wound tissue in rats. Methods: Laboratory experimental design with a total sample size of 27 male rats have undergone incisions, divided into 3 random groups: control group was given aquadest, treatment group 1was given aquadest and 50% extract of snakehead fish, and treatment group 2 was given 100% extract of snakehead fish. The rat’s skin wound tissues were taken on day 5 and observed under the microscope. The average number of macrophages and blood vessels on granulation tissue that formed at the base of the wound were counted and then performed data analysis. Result: In this study, we found a down wards trend in the average number of macrophages and blood vessels in treatment group 1 and treatment group 2. The control group and the treatment group 2 showed a significant difference, however, between the other groups did not show significant difference. Conclusion: There is a significant effect of giving 100% snakehead fish extract on decreased macrophages and blood vessels in the inflammatory reaction of skin wound tissue.

Profile of Skin Biopsy Patients in Dr. Soetomo General Academic Hospital, Surabaya, Indonesia R.A. Astrid Putri Wandhita; Willy Sandhika; M. Yulianto Listiawan
Folia Medica Indonesiana Vol. 58 No. 1 (2022): March
Publisher : Universitas Airlangga

Highlight:Profile of skin biopsy results which carried out the anatomical pathology examination was reviewed.The highest skin biopsy disease group case are erythropapulosquamous, infection, skin tumor, vesiculobullous, connective tissue disease, pigmentation disorders, and vasculitis Abstract:Skin biopsy is an important tool used by dermatologists in diagnostic determination. The correlation between clinical and histological features is needed in understanding pathogenesis and formulating the diagnosis of a skin disease with a greatly varied spectrum of histopathological results, while the observable clinical symptoms are highly limited. Skin diseases are still a serious problem worldwide, especially in Indonesia. Based on the Indonesian Health Profile in 2010, skin diseases ranked third out of 10 most diseases in outpatients in hospitals throughout Indonesia. This study was a review of the profile of skin biopsy results in Dr. Soetomo General Academic Hospital, Surabaya, Indonesia from 1 July 2014 to 31 July 2019, which were subjected to anatomic pathology examination. This study was an observational descriptive study using secondary data sources from the medical records at the Communication and Information Technology Installation (ICT) of Dr. Soetomo General Academic Hospital, Surabaya. Based on data searches, the total number of biopsy examinations performed was 1,368 cases. There were more female patients (50.3%) than males (49.7%). The most common skin disorder found was erythropapulosquamous disorder (30%), followed by infection (18%). Other cases consisted of skin tumor (15%), vesiculobullous (13%), connective tissue disease (7%), pigmentation disorders (5%), and vasculitis (5%). Diseases that could not be classified into 7 groups of the biopsy criteria were grouped separately in other diseases (7%).

EVALUASI JARINGAN DALAM STUDI IN VIVO PEMBERIAN EKSTRAK PROPOLIS PADA MODEL LUKA INFEKSI Winona May Hendrata; Willy Sandhika; Agung Dwi Wahyu Widodo
Majalah Biomorfologi Vol. 29 No. 2 (2019): Majalah Biomorfologi
Publisher : Universitas Airlangga, Surabaya, Indonesia

Background: Infection in wound could lead to various consequences including scarring and occurrence of sepsis. With increasing prevalence of resistant strain bacteria, option for treating infected wound need to be constantly expanded. Propolis, a product of honey bee, is a potential treatment which has been studied to have various effects including antibacterial, anti-inflammatory, and antioxidant. Objective: The study aims to assess the effect of propolis toward wound healing, by evaluating wound macroscopic and microscopic appearance. Material and method: Propolis extract were made using maceration technique using ethanol. Thirty Rattus norvegicus male rats were divided into six groups: three groups for treatment and three groups for control. Incision wound were made in the back and was infected with Methicillin ResistantStaphylococcus aureus (MRSA). Propolis was administered orally on daily basis. Wound excision for observation were done on day3, 5, and 7. The tissue were processed using haematoxylin-eosin (HE) staining for microscopic observation. The distance of normal tissue surrounding the wound were measured using image analysis program. Result: Macroscopic observation showed fewer visible wound in the propolis extract treated group. Measurement by microscopic observation on day 3 also demonstrated more narrow wound width in propolis extract treated group (405.75  110.11 μ) compared to the control group (806.20 644.41μ). Conclusion: The result of this study suggests that propolis extract by oral administration promotes the healing of infected wound as shown within microscopic and microscopic observation.

Co-Authors A.A. Ketut Agung Cahyawan W Afif Nurul Hidayati, Afif Nurul Agung Dwi Wahyu Widodo Agustin Nurliani Aisha Grayli Cahyani Alief Yudo Astuti Alphania Rahniayu Anny Setijo Rahaju, Anny Setijo Ariani, Grace Aries Sasongko Astindari Astindari Astindari Astindari, Astindari Ayu Tyasmara Bagus Haryo Kusumaputra, Bagus Haryo Baiq Ratna Kumaladewi Bernadetta Jonan Betty Agustina Tambunan Brama Rachmantyo Budiono Budipramana, Vicky Sumarki Cita Rosita Sigit Prakoeswa Densy Violina Harnanti Dian Eskaningrum Djoni Susanto Dwi Murtiastutik DYAH FAUZIAH, DYAH Endi Prilansa Mahadi Erlina Erlina Faizah, Zakiyatul Fanny Gunawan Fertilita, Soilia Firda Azizah Gatut Hardianto, Gatut Gilda Hartecia Gondo Mastutik Grace Ariani Hartanto, Felix Hartecia, Gilda Heny Arwati Heriyawati, Heriyawati Heryawati Heryawati I Wayan Yudiana Ibrahim, Azarya Ida Septika Wulansari Ilmiah, Khafidhotul Imam Susilo INDROPO AGUSNI Irene Lingkan Parengkuan Irmadita Citrashanty, Irmadita Isnin Anang Marhana Iswinarno Doso Saputro iwan Syarif Jimmy Hadi Widjaja Jonan, Bernadetta Junita Jeanne Paliman Kartika Arum Wardani Kholida Nur Aini Kiki Apnita Sari Kintan Putri KUSUMASTUTI, ETTY HARY LaleMaulin Prihatina Linda Astari, Linda M. Yulianto Listiawan Mappamasing, Hasnikmah Marina Rimadhani Marina Rimadhani Martha Kurnia Kusumawardani Maylita Sari Maylita Sari, Maylita Meita Ardini Pratamasari, Meita Ardini Muhammad Miftahussurur Nanda Amalia Ramadhanti Navisa, Claudia Clary Nidya Ulfana Tsania Nila Kurniasari Novia Indriyani Adisty Nurina Hasanatuludhhiyah Nurul Wiqoyah, Nurul Poernomo, Adinda Sandya PUNGKY MULAWARDHANA, PUNGKY Purwati Purwati Purwati Puteri, Agnes Ilene Suprapto Qonitatillah, Ana R. Heru Prasetyo R.A. Astrid Putri Wandhita Rahel Yuana Sadikim Ratna Darjanti Haryadi Regina Martina Cilik Ridholia, Ridholia Rizaldi, Fikri Rulisiana Widodo Rusdamayanti Rusdamayanti Rwahita Satyawati Dharmanta Ryski Meilia Novarina Ryski Meilia Novarina, Ryski Meilia S.Pd. M Kes I Ketut Sudiana . Sabilal Alif Sari, Aditya Sita Sari, Festi Artika savitri, Lisa Sawitri Sawitri Sawitri Sawitri Sawitri Soilia Fertilita Sunarso Suyoso Sunaryo Hardjowijoto Suprabawati, Desak Gede Agung Tessy Badriyah, Tessy Theresia Fifi Judikristiani Tri Hartini Yuliawati Trisniartami Setyaningrum Tyasmara, Ayu Umi Choviva, Farida Ummi Maimunah Vinna Chrisdianti Widati Fatmaningrum Wilda Fitria Rachmadina Windya Tri Hapsari Winona May Hendrata Wiratama, Priangga Adi yasmin, dzakiyah Yusfita Evi Rosdiana

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