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Irda Sayuti
Pendidikan Biologi Universitas Riau
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Journal : JURNAL BIOLOGI PAPUA

 1 
Ekstraksi DNA Nibung (Oncosperma tigillarium (Jack) Ridl.) Menggunakan Beberapa Jenis Buffer Terhadap Kualitas Genomik DNA Mahadi, Imam; Sayuti, Irda; Nursal, Nursal
Jurnal Biologi Papua Vol 16 No 2 (2024)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/jbp.3780

Abstract

The Nibung (Oncosperma tigillarium (Jack) Ridl.) is a plant that serves as the floral mascot of Riau and symbolizes the spirit of unity and brotherhood among its people. Genetic diversity research is essential for the conservation of this species, with DNA extraction being a critical initial step. This study compared three types of buffers: CTAB, Alkyl Benzene Sulfonate (ABS) surfactant detergent, and Sodium Laureth Sulfate (SLS) surfactant detergent. The aim of this research was to identify alternative buffers to replace CTAB and simplify the extraction of genomic DNA from Nibung. Parameters measured included the presence of DNA and DNA concentration, using electrophoresis and a nanodrop spectrophotometer. The results showed that clear genomic DNA bands were successfully obtained for 24 Nibung accessions, with only two accessions, Nibung2s and Nibung6s, showing smeared bands when using the SLS surfactant detergent buffer. The genomic DNA concentrations ranged from 526.2 to 3,829.2 ng/µl. These concentrations are suitable for use as DNA templates in further research. Thus, both ABS and SLS surfactant detergent buffers can be considered viable alternatives to CTAB for extracting genomic DNA from Nibung.  Key words: Buffer; DNA bands; DNA extraction; DNA Concentration; Extraction optimization.
Co-Authors ', Darmawati ', Haryati ', Johan ', Jumari ', Nusrsal ', Suratni ', Yustina AA, Robi Komari Adila Ulfa Pulungan Adrian Hartanto, Adrian Afridayanti, Sintya Al Farazi Alfi Rahmah Alfirahmani Alfirahmani Angga Irama Anitia Anitia Ardelia Naisya Agustina Ardiani, Dilla Arie Ferdinandsyah Arnentis Arnentis Arnentis Arnentis Arnentis, Arnentis Ashari, Mauliza Asnia Ayu Lestari Audi Maia Rachman Aura Sandra Ayu Wahyuni Butar-Butar, Istami Hiqma Cahyati, Nindy Inka Citra Fajri Darmadi Darmadi Darmawati ' Darmawati ' ' Darmawati Darmawati Darmawati Darmawati Deci Ririen Dede Gusdiana Desi Putri Ayu Desrianty Syafitri Devi Suryawati Dian Astri Maulani Dian Kurnia Sari Dian Munawarah Dianti, Putri Rahma Dipuja, Diah Anugrah Effendi ' Elisabeth Elisabeth Elsa Safitri Eva Rahmayanti Lubis Evi Suryawati Evi Suryawati Evi Suryawati Suryawati, Evi Suryawati Fajriyah Wirawati Febblina Daryanes Ferdian Yudha Pratama Fikri, Khusnul Fitra Suzanti Fransiska Flonia Gevin Alexandro Gusti Ajeng Ayuni Gustri Musdalifah Habibah, Irma Hamdi Harmanzah Hardianti, Nia Harlis Harlis Hendrawan, Reza Huseno, Tun Iasah, Bella Imam Mahadi Indah Maya Istanti Indriawati, Siska Ira Wulandari Irma Habibah Isra Layla Yusti Layla Yusti, Isra Layla Yusti Layla Jamhari Jamhari Jumari Jumari, Jumari Kamarudin, Kasrina Kayla Annasya Varandra Khasanah, Nurul Uswatun Levi Maryani, Levi Lisa Indriani Lisa Yuka Ramadhani Lucky Manuel Sihotang M. Irsyad Nur Mariamlie Mariamlie Mariani Natalina Mariani Natalina Linggasari Mariani Natalina Linggasari Marni Srihartati Harefa Marsela Wulandari Maryani, Desi Meilianti, Dasni Elsa Mellisa, Novyona Ayu Mida Wulandari Mutia Adlina Rifani Nadila Berliani Nia Hardianti Novita Sri Wahyuni Sri Wahyuni Novita, ' Novyona Ayu Mellisa Nurhasanah ' Nurhasanah ' ' Nursal Nursal Nursal Nursal Nursal Nursal Nursal Nursal, Nursal Nusrsal ' Nuur Badriyyah Akmal Orindu Renjani Putri Dini Dameria Putri Titania Salsabila Rafiqa Ilma Dini Rahmat Ali Bahri Rahmi Yulia Putri Rahmi, Zunnur Raja Sonang Reza Hendrawan Ria Dess Septi Laseira Ria Rezki Oktafina Ridho Safna Rika Mulyani Risa Imanda Robi Komari AA Rosmaini S Sahnari Tambunan Sara Gleri Lubis Satifa, Nabela Ida Coriza Selvya Nadilla Setia Febriani Hammy Sheliana, Yolanda Shintia Oktaviani Siagian, Lili Rahma SITI FATIMAH Siti Nurhaliza Sri Andayannhi SRI RAHAYU Sri Wulandari SRI WULANDARI Sri Wulandari Sri Wulandari Sri Wulandari Wulandari, Sri Wulandari Sumarni ' Suratni " suwondo suwondo Suwondo Suwondo Syafi’i, Wan Syafitri, Desrianty Tedy Akmal Hernando Trisa Novia Sari Utari, Melita Viona Sephia Putri Wan Safii Wan Syafii Wan Syafi’i Widodo, Teguh Juliantani Widya Astariana Wirawati, Fajriyah Yola Aprila Yollanda Susilawati Yuslim Fauziah Yustina " Yustina Yustina Zatri Rahayu Zulfaida Kurni Aurianti Zulfarina Zulfarina ' ' Zulfarina Zulfarina Zulfarina Zulfarina Zulfarina, Zulfarina