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All Journal Jurnal Ilmu Lingkungan Jurnal Pendidikan Jurnal Pilar Sains Jurnal Online Mahasiswa (JOM) Bidang Keguruan dan Ilmu Pendidikan SEMIRATA 2015 Jurnal Agro Proceeding 7th International Seminar on Regional Education Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosfer: Jurnal Pendidikan Biologi Proceedings of the 1st UR International Conference on Educational Sciences Diklabio: Jurnal Pendidikan dan Pembelajaran Biologi JMM (Jurnal Masyarakat Mandiri) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Pembelajaran dan Biologi Nukleus Maspul Journal of Community Empowerment IJOEP : International Journal of Ecophysiology Unri Conference Series: Community Engagement Jurnal Pengabdian kepada Masyarakat Jurnal Jeumpa BIOGENESIS Journal of Educational Sciences JURNAL BIOLOGI PAPUA Faedah: Jurnal Hasil Kegiatan Pengabdian Masyarakat Indonesia Indonesian Journal of Environment and Disaster International Journal of Educational Best Practices Biodik: Jurnal Ilmiah Pendidikan Biologi Kolaborasi: Jurnal Hasil Kegiatan Kolaborasi Pengabdian Masyarakat
Irda Sayuti
Pendidikan Biologi Universitas Riau
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Other

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Journal : Jurnal Agro

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Kultur suspensi sel tanaman gajah beranak (Goniothalamus tapis Miq) terhadap kandungan zat goniotalamin Imam Mahadi; Sri Wulandari; Wan Safii; Irda Sayuti
Jurnal Agro Vol 8, No 2 (2021)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/14710

Abstract

Zat goniotalamin pada tanaman gajah beranak (Goniothalamus tapis) merupakan obat alternatif penyembuhan kanker. Penelitian bertujuan untuk mendapatkan zat goniotalamin melalui kultur kalus dan kultur suspensi sel. Metode penelitian eksperimen Rancangan Acak Lengkap (RAL) dengan kombinasi 2,4-D (1-10 mgL-1) dan BAP (0,5-2 mgL-1) menggunakan eksplan batang muda, terdiri dari 17 perlakuan dan 3 kali ulangan. Analisis data menggunakan Analysis of Variances dan uji lanjut Duncan Multiple Range Test (DMRT) taraf 5%. Hasil menunjukkan bahwa kultur kalus G. tapis pada media 5,0 mg L-1 2,4-D + 1 mg L-1 BAP adalah yang terbaik dengan waktu muncul kalus 28,33 hari dan persentase pembentukan kalus 100%. Kalus untuk kultur suspensi sel bertekstur remah dan berwarna kuning kehijauan. Kultur suspensi sel menghasilkan pertumbuhan sel yang cepat, tidak lembek berair dan mudah dipisahkan. Hasil kualitatif Kromatografi Lapis Tipis kultur suspensi sel sangat jelas, bersih dan terdapat potensi kandungan zat goniotalamin pada perlakuan 2,4-D 5 mg L-1 + BAP 0,5 mg L-1, 2,4-D 5 mg L-1 + BAP 1 mg L-1, 2,4-D 5 mg L-1 + BAP 2 mg L-1, 2,4-D 10 mg L-1 + BAP 0,5 mg L-1 dan 2,4-D 10 mg L-1 + BAP 1 mg L-1. Hasil kuantitatif zat goniotalamin dengan Kromatografi Cair Prestasi Tinggi terdapat pada perlakuan 2,4-D 5,0 mgL-1 + BAP 1 mg L-1 yaitu 9,57 mg g-1.The goniothalamine compound on Goniothalamus tapis is an alternative cancer medicine. This study aimed to obtain gonotalamin through callus culture and suspension cell culture. The experiment research method was Completely Randomized Design (CRD) with a combination of 2.4-D (1-10 mg L-1) and BAP (0.5-2 mg L-1) using young stem explants consisting of 17 treatments with 3 replications. Data analysis used ANOVA and DMRT at 5%. The results showed that G. tapis callus culture on 5.0 mg L-1 2.4-D + 1 mg L-1 BAP was the best treatment medium with callus emergence time of 28.33 days and percentage of callus formation 100%. The callus used for suspension cell culture was friable and greenish-yellow in color. Suspension cell culture resulted in rapid cell growth, was not fleshy, and easily separated. The  quality test by Thin Layer Chromatography (TLC) from suspension cell culture resulted very clear, clean, and potential content of goniothalamin found in treatments 2.4-D 5.0 mg L-1 + BAP 0.5 mg L-1, 2.4-D 5.0 mg L-1 + BAP 1 mg L-1, 2.4-D 5.0 mg L-1 + BAP 2 mg L-1, 2.4-D 10 mg L-1 + BAP 0.5 mg-1 and 2.4-D 10 mg-1 + BAP 1 mg-1. The quantitative results of the best goniotalamine compounds in cell suspension cultures using High-Performance Liquid Chromatography (HPLC) on medium 2,4-D 5.0 mgL-1 + BAP 1 mg L-1 ie 9.57 g-1.
Co-Authors ', Darmawati ', Haryati ', Johan ', Jumari ', Nusrsal ', Suratni ', Yustina AA, Robi Komari Adila Ulfa Pulungan Adrian Hartanto, Adrian Afridayanti, Sintya Al Farazi Alfi Rahmah Alfirahmani Alfirahmani Angga Irama Anitia Anitia Ardelia Naisya Agustina Ardiani, Dilla Arie Ferdinandsyah Arnentis Arnentis Arnentis Arnentis Arnentis, Arnentis Ashari, Mauliza Asnia Ayu Lestari Audi Maia Rachman Aura Sandra Ayu Wahyuni Butar-Butar, Istami Hiqma Cahyati, Nindy Inka Citra Fajri Darmadi Darmadi Darmawati ' Darmawati ' ' Darmawati Darmawati Darmawati Darmawati Deci Ririen Dede Gusdiana Desi Putri Ayu Desrianty Syafitri Devi Suryawati Dian Astri Maulani Dian Kurnia Sari Dian Munawarah Dianti, Putri Rahma Dipuja, Diah Anugrah Effendi ' Elisabeth Elisabeth Elsa Safitri Eva Rahmayanti Lubis Evi Suryawati Evi Suryawati Evi Suryawati Suryawati, Evi Suryawati Fajriyah Wirawati Febblina Daryanes Ferdian Yudha Pratama Fikri, Khusnul Fitra Suzanti Fransiska Flonia Gevin Alexandro Gusti Ajeng Ayuni Gustri Musdalifah Habibah, Irma Hamdi Harmanzah Hardianti, Nia Harlis Harlis Hendrawan, Reza Huseno, Tun Iasah, Bella Imam Mahadi Indah Maya Istanti Indriawati, Siska Ira Wulandari Irma Habibah Isra Layla Yusti Layla Yusti, Isra Layla Yusti Layla Jamhari Jamhari Jumari Jumari, Jumari Kamarudin, Kasrina Kayla Annasya Varandra Khasanah, Nurul Uswatun Levi Maryani, Levi Lisa Indriani Lisa Yuka Ramadhani Lucky Manuel Sihotang M. Irsyad Nur Mariamlie Mariamlie Mariani Natalina Mariani Natalina Linggasari Mariani Natalina Linggasari Marni Srihartati Harefa Marsela Wulandari Maryani, Desi Meilianti, Dasni Elsa Mellisa, Novyona Ayu Mida Wulandari Mutia Adlina Rifani Nadila Berliani Nia Hardianti Novita Sri Wahyuni Sri Wahyuni Novita, ' Novyona Ayu Mellisa Nurhasanah ' Nurhasanah ' ' Nursal Nursal Nursal Nursal Nursal Nursal Nursal Nursal, Nursal Nusrsal ' Nuur Badriyyah Akmal Orindu Renjani Putri Dini Dameria Putri Titania Salsabila Rafiqa Ilma Dini Rahmat Ali Bahri Rahmi Yulia Putri Rahmi, Zunnur Raja Sonang Reza Hendrawan Ria Dess Septi Laseira Ria Rezki Oktafina Ridho Safna Rika Mulyani Risa Imanda Robi Komari AA Rosmaini S Sahnari Tambunan Sara Gleri Lubis Satifa, Nabela Ida Coriza Selvya Nadilla Setia Febriani Hammy Sheliana, Yolanda Shintia Oktaviani Siagian, Lili Rahma SITI FATIMAH Siti Nurhaliza Sri Andayannhi SRI RAHAYU SRI WULANDARI Sri Wulandari Sri Wulandari Sri Wulandari Sri Wulandari Wulandari, Sri Wulandari Sumarni ' Suratni " suwondo suwondo Suwondo Suwondo Syafi’i, Wan Syafitri, Desrianty Tedy Akmal Hernando Trisa Novia Sari Utari, Melita Viona Sephia Putri Wan Safii Wan Syafii Wan Syafi’i Widodo, Teguh Juliantani Widya Astariana Wirawati, Fajriyah Yola Aprila Yollanda Susilawati Yuslim Fauziah Yustina " Yustina Yustina Zatri Rahayu Zulfaida Kurni Aurianti Zulfarina Zulfarina ' ' Zulfarina Zulfarina Zulfarina Zulfarina Zulfarina, Zulfarina