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All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan ACTA VETERINARIA INDONESIANA Jurnal Pengolahan Hasil Perikanan Indonesia Hemera Zoa Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Medical Journal of Indonesia Current Biochemistry Jurnal Ilmu Ternak Veteriner Warta IHP (Warta Industri Hasil Pertanian) Historika Jurnal Medik Veteriner ARSHI Veterinary Letters FITOFARMAKA: Jurnal Ilmiah Farmasi Majalah Patologi Indonesia Indonesian Journal of Applied Research (IJAR) International Journal of Multidisciplinary: Applied Business and Education Research Journal of Education Research Jurnal Pusat Inovasi Masyarakat Jurnal Kedokteran Hewan Journal of Research and Publication Innovation VALUTA Jurnal Administrasi Bisnis, Manajemen dan Ekonomi Policy Brief Pertanian, Kelautan, dan Biosains Tropika Health Gate Jurnal Agripet Seminar Nasional Riset dan Teknologi (SEMNAS RISTEK)

Agus Setiyono

Departemen Klinik, Reproduksi Dan Patologi Fakultas Kedokteran Hewan, Institut Pertanian Bogor. Jl. Agatis Kampus IPB Dramaga 16680

Author-ID : 377503

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Deteksi Coxiella burnetii pada Sapi Kurban Idhul Adha di Cimanggu, Kabupaten Bogor Tahun 2015-2016 (DETECTION OF COXIELLA BURNETII IN IED AL-ADHA SACRIFICIAL COW IN CIMANGGU, BOGOR REGENCY AT 2015-2016) Handayu Untari; Agus Setiyono; Ekowati Handharyani; Masdiana C. Padaga
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Coxiella burnetii is an intracellular obligate bacterium that causes Q fever disease. Q fever is one of the asymptomatic zoonosis and has a potential as biological weapon. This disease infects much type of animals and has been distributed globally, including in Indonesia. Previous research concluded that West Java is an endemic area of this disease. Bogor in West Java has a potential spread of Q fever disease due to high population of cattle and goat in this region.This study aim was to detect C. burnetii in spleen, lung, kidney, liver and heart of cows. Samples of were taken from a total of 29 sacrificial cows during Eid al-Adha in 2015 and 2016 in the Cimanggu region, Bogor Regency. The examination method used was immunohistochemistry with primary polyclonal antibody Rabbit anti-C. burnetii and HaematoxylineEosine staining. The results of the immunohistochemistry examination showed positive immunoreaction (specific brown color in the cytoplasm of cells) against C. burnetii antibodies respectively in 3 out of 10 samples in 2015 (30%) and 4 out of 19 samples in 2016 (21%). Out of 29 samples, positive immunoreaction were found in lung organ (13,7%) and spleen (10,3%) with variation in changes in histopathological features which include congestion, inflammatory cell infiltration, mucus accumulation, and fibrosis in the lung.

Deteksi Coxiella burnetii Penyebab Q fever pada Sapi, Domba dan Kambing di Bogor dan Bali (DETECTION OF COXELLA BURNETII, THE CAUSAL AGENT OF Q FEVER Hapsari Mahatmi; Agus Setiyono; Retno Damayanti Soejoedono; Fachriyan Hasmi Pasaribu
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study to detect Coxiella burnetii, an intracellular bacterium causing Q fever in human and livestock animals, was carried out in several ruminants in Bogor and Bali. The methods used for the detection was Nested-Polymerase Chain Reaction (Nested-PCR). Two pairs of primers, the first (OMP1 and OMP2) and the second (OMP3 and OMP4) were used to detect the genomic sequences and the conserved specific sequences of Coxiella burnetii, respectively. Organ samples such as liver and lung from 410 livestock ruminants, consisting of cattle (245 samples), sheep (105 samples) and goats (60 samples) were collected from several slaughter houses in Bogor and Bali. As many as 15 (6.12%) out of 245 cattle, 6 (5.71%) out of 105 sheep and none from goat were infected by Coxiella burnetii. Interestingly, 3 out of 15 infected cattle were Bali cattle. The results clearly indicate that Q fever is likely to be widespread among ruminant animals in Indonesia.

Profil Kinetik dan Efektivitas Enrofloksasin yang Dikombinasikan dengan BioATP dalam Mengatasi Coxiella burnetii (KINETIC PROFILE AND EFFECTIVITY OF ENROFLOXACINE WITH BIO ADENOSIN TRIPHOSPHATE SUPPLEMENTATION AGAINST COXIELLA BURNETII) Andriyanto .; Agus Setiyono; Min Rahminiwati; Neni Nuryani; Unang Patriana
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Coxiella burnetii belongs to rikettsia group living obligate intracellularly and as the agent of zoonosisQ fever. Enrofloxacine is an antibiotic in quinolon group used to treat infection of C. burnetii in chicken,goat, calve, pig, dog, cat, and horse. From ruminant practical experience, enrofloxacine if combined withBioATP can enhance the enrofloxacine activity. Research for the effecivity of enrofloxacine and BioATP totreat C. burnetii has never been carried out. The research was conducted to explore effect of enrofloxacinewith supplementation BioATP against C. burnetii. Enrofloxacine pharmacokinetic study was carried outby using simental beef as an experimental animals. The effectivity of BioATP supplementation onenrofloxacine activity to treat C. burnetii was tested by using Vero cell tissue culture. The results showedthat combination of enrofloxacine and BioATP increased kinetic profile of enrofloxacine in term of onset,duration, pharmacology intensity, and bioavailaibility. Enrofloxacine had activity to treat C. burnetii withvalue of minimal inhibitory concentration (MIC) at 1-2 ppm and value of minimal bactericidal concentrationat 4 ppm. Supplementation of BioATP improved the effectivity of enrofloxacine in treating C. burnetii.

Sensitivitas dan Spesifisitas Nested Polymerase Chain Reaction untuk Mendeteksi DNA Coxiella burnetii (SENSITIVITY AND SPECIFICITY OF NESTED POLYMERASE CHAIN REACTION FOR DETECTION OF COXIELLA BURNETII DNA) Trioso Purnawarman; I Wayan Teguh Wibawan; Fachriyan Hasmi Pasaribu; Agus Setiyono; Muharam Saepulloh
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Sensitivity and specificity of nested polymerase chain reaction (nested PCR) to detect Coxiella burnetii(C. burnetii) DNA were studied. The primer system which consists of external primers (OMP1 and OMP2)and internal primers (OMP3 and OMP4), was designed from the nucleotide sequence of the com I geneencoding for 27 kDa outer membrane protein and used to specifically amplify a 501 bp and 438 bp fragment.This nested PCR assay was 50 fold more sensitive than that of using PCR external primer only. TheNested PCR has a detection limit as low as 300 pg/?l. Specificity studies showed that nested PCR onlydetected C. burnetii DNA and did not happened Brucella abortus, Escherichia coli, Pseudomonas aeruginosaand Campylobacter Jejuni DNA. Nested PCR has high senstively and specificaly diagnostic method of C.burnetii as agent of Q fever disease.

Aktivitas Penurunan Kadar Glukosa pada Tikus yang Diinduksi Aloksan dari Ekstrak Air Angkak, Bekatul, dan Kombinasinya Hasim Hasim; Didah Nur Faridah; Mega Safithri; Husnawati Husnawati; Agus Setiyono; Hanif Alamudin Manshur
Warta Industri Hasil Pertanian Vol 37, No 2 (2020)
Publisher : Balai Besar Industri Agro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32765/wartaihp.v37i2.5460

ABSTRAK: Angkak dan bekatul mengandung komponen bioaktif sebagai antihiperglikemik. Namun penelitian mengenai aktivitas antihiperglikemik angkak dan bekatul secara in vivo belum tersedia. Tujuan penelitian ini adalah menguji efektivitas pemberian ekstrak air angkak, ekstrak air bekatul serta kombinasinya dalam proses penurunan jumlah glukosa dalam darah tikus (Rattus norvegicus) strain Sprague Dawley yang diinduksi aloksan. Penelitian dilakukan dengan melakukan preparasi sampel yaitu ekstraksi air angkak dan bekatul, pengkondisian hewan uji yaitu tikus (Rattus norvegicus) strain Sprague Dawley dengan induksi aloksan, pembagian tikus menjadi sembilan kelompok. Hasil penelitian menunjukkan penurunan kadar glukosa darah tikus paling rendah adalah pada tikus kelompok V (angkak 2) dengan pemberian ekstrak air angkak 100 mg/Kg bb, dengan penurunan 61,43% lebih tinggi dibandingkan dengan kelompok III, tikus yang diberi obat glibenklamid yang turun sebesar 40,88%. Pada kelompok V, kadar glukosa darah pada hari ke-3 sebesar 256,67±174,77 mg/dL dan turun pada hari ke-12 hingga mencapai glukosa darah normal sebesar 99,00±9,64 mg/dL. Penurunan kadar glukosa darah tikus baik kelompok pemberian ekstrak bekatul maupun dengan kombinasi ekstrak air angkak dan ekstrak air bekatul tidak menunjukkan penurunan kadar glukosa darah yang signifikan.Kata kunci: aloksan, angkak, bekatul, glukosa darahABSTRACT: Bioactive compounds in red yeast rice (traditionally known as angkak) and rice bran are scientifically evidenced to exert antihyperglycemic properties. Nevertheless, in vivo studies on antihyperglycemic activity of red yeast rice and rice bran have not been reported. This current work aimed to evaluate glucose-lowering effects on alloxan-induced rats (Rattus norvegicus) strain Sprague Dawley treated with water extract of angkak, rice bran and combination of both extracts. The research was first carried out by extraction of RYR and RB, acclimatization of rats induced by alloxan, then separating them into 9 groups. The results showed that the lowest decrease in blood glucose, i.e. 61,43%, was attributed to group 5, namely rats administered with water extract of angkak (100 mg/Kg bw), being much higher compared to group 3 (rats treated with glibenclamide) reaching up to 40,88%. In terms of group 5, the level of blood glucose exhibited a gradual attenuation, from 256,67±174,77 mg/dL on day-3 to 99,00±9,64 mg/dL on day-12, which is a normal blood glucose concentration. It is noteworthy that administration of rice bran extract and combined treatment showed no significant effects on reduction of blood glucose.Keywords: alloxan, blood glucose, red yeast rice, rice bran, antihyperglycemic

ANTIBREAST CANCER ACTIVITY OF NANOPROPOLIS INDONESIA ON INDUCED MAMMARY GLAND TUMOR BY DMBA IN VIRGIN SPRAGUE-DAWLEY RATS Akhmad Endang Zainal Hasan; Djumali Mangunwidjaja; Titi Candra Sunarti; Ono Suparno; Agus Setiyono
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 23 No. 1 (2016)
Publisher : SEAMEO BIOTROP

The objective of this study was to determine the effect of nanopropolis to cure cancer induced on rat mammary tumor using 7,12-dimethylbenz(a)anthracene (DMBA). After the first tumors appearance, twenty eight rats were divided into seven groups. Group 1, 2 and 3 served as recipient of nanopropolis dosages 8, 32 and 56 Âµg/mL treatments; Group 4 served asrecipient of propolis dosage of 233 Âµg/mL treatment; Group 5 served asrecipient of doxorubicin treatment; Group 6 served as recipient of DMBA treatment and Group 7 as normal group (control). The effect of nanopropolis dosage of 32 Âµg/mL and propolis dosage of 233 Âµg/mL were similar in reducing tumor size, healing the wounds caused by the tumor and eliminating cancer cells. It turns out that there is a relationship between particle size absorbent materials. The study suggested that nanopropolis with small concentration was very effective to treatrat mammary gland tumors and breast cancers.Keywords: breast cancer, nanopropolis, propolis, Sprague-Dawley rat

KONDISI HATI TIKUS BETINA AKIBAT INDUKSI 7,12-DIMETHYL BENZ(?)ANTHRASEN (DMBA) DAN PENYEMBUHANNYA DENGAN PROPOLIS DAN NANOPROPOLIS INDONESIA Akhmad Endang Zainal Hasan; E. Mulyati Effend; Agus Setiyono; Bayu Sandi
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 4, No 1 (2014): FITOFARMAKA
Publisher : Universitas Pakuan

Tujuan dari penelitian ini adalah menentukan efek farmakologis propolis dan nanopropolis untuk pengobatan penyakit hati pada tikus betina yang diinduksi senyawakarsinogenik 7,12 - dimetilbenz(?)antasena (DMBA). Penelitian dilakukan dengan mengamatihistopatologi dan makroskopik hati pada 28 ekor tikus betina galur Sprague - Dawley. Tikuspercobaan dibagi menjadi 7 kelompok perlakuan dengan 6 kelompok yang diinduksi DMBA(Kelompok I- VI ) dan 1 kelompok sebagai kontrol normal. Kelompok I sebagai kontrolnegatif diberi 1 ml NaCl secara injeksi intraperitoneal (ip). Kelompok II - IV diberinanopropolis 8; 32 dan 56 ppm ip. Kelompok V diberi ekstrak ethanol propolis 233 ppm ip,kelompok VI sebagai kontrol positif diberikan doxorubixin ip dan kelompok VII sebagaikontrol normal diberi penyediaan akuades. DMBA diinduksi selama 11 minggu danpengobatan dilakukan 15 minggu. Setiap minggu tikus ditimbang bobotnya dan diperiksaterhadap inisiasi tumor. Hasil penelitian menunjukkan bahwa ekstrak etanol propolis 233 ppmdan nanopropolis konsentrasi 32 dan 56 ppm dapat mempertahankan kondisi optimal hatitikus. Efeknya adalah setara dengan kontrol normal.

SUPLEMENTASI KELAPA KOPYOR TERHADAP AKTIVITAS SUPEROKSIDA DISMUTASE DAN PATOLOGI ANATOMI HATI TIKUS AKIBAT PERLAKUAN PARASETAMOL Akhmad Endang Zainal Hasan; Hasim Hasim; Agus Setiyono; Sekar Winahyu Ariadini
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 3, No 1 (2013): FITOFARMAKA
Publisher : Universitas Pakuan

This research aimed at determining of the effects of the induction of paracetamoland kopyor coconut on the activity of superoxide dismutase (SOD) and pathologicanatomy of rats liver. Previous research shows that paracetamol caused negative effectto the body while kopyor coconut has the ability to improve human health, related to itscapacity to prevent oxidative damage of human body. In this research, the positiveeffect of kopyor is approached through measurement of body weight and the activity ofthe total SOD enzyme of animals tested, which are induced by paracetamol and fed withkopyor. Rats administered with temulawak were positive control. The result showedthat paracetamol treatment using toxic dose was found to reduce body weight of testedanimals. Meanwhile, both kopyor and temulawak supplements were able to counter thenegative effect of paracetamol, indicated by the increase in the body weight of testedanimals. Other results showed that treatments with temulawak and 5 times-dose ofkopyor supplement demonstrated a significantly high total SOD activity in the liver ascompared to negative control. Furthermore, pathologic observation of the testedanimals liver showed that 5 time-dose of kopyor treatment was confirmed to improve the recovery of the liver caused by negative effect of paracetamol, better than the negative control.Key words: kopyor coconut, superoxide dismutase, free radical, pathologic

Infeksi Anisakid pada lumba-lumba hidung botol indo-pasifik (Tursiops aduncus) di situs konservasi lumba-lumba, Indonesia Ismah Atika Salmah; Risa Tiuria; Agus Setiyono; Tri Isyani Tungga Dewi
ARSHI Veterinary Letters Vol. 2 No. 4 (2018): ARSHI Veterinary Letters - November 2018
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Penyakit parasit dapat mempengaruhi kesehatan lumba-lumba yang dipelihara di konservasi. Informasi berkaitan dengan jenis parasit yang menginfeksi lumba-lumba di konservasi masih sangat terbatas. Tujuan penelitian ini adalah untuk mengidentifikasi parasit lumba-lumba hidung botol indo-pasifik (Tursiops aduncus) di situs konservasi lumba-lumba, Indonesia. Sampel feses diambil secara langsung dari rektum menggunakan rubber catheter. Sampel feses dilakukan pemeriksaan dengan metode pengapungan sederhana, filtrasi dan sedimentasi, serta metode Baermann. Hasil dari pemeriksaan sampel feses menunjukkan satu dari tiga lumba-lumba hidung botol indo-pasifik (Tursiops aduncus) terinfeksi Anisakid. Hasil tersebut menunjukkan perlunya dilakukan pengendalian parasit di situs konservasi lumba-lumba.

Infestasi Caplak Ixodidae pada Sapi Lokal di Kelurahan Balai Gadang Kecamatan Koto Tangah Kota Padang Aulia Dina Kristina; Agus Setiyono
Jurnal Pusat Inovasi Masyarakat (PIM) Vol. 2 No. 2 (2020): April 2020
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Institut Pertanian Bogor

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Abdi Nusantara XIII is a facility for students of the Faculty of Veterinary Medicine IPB University as part of social change agents to realize the third Tri Dharma of Higher Education, namely the Community Service Dharma. One of the activities of Abdi Nusantara XIII have been held using the method of observation and active participation in 18 July 2019 with Puskeswan Air Pacah under the Department of Agriculture of Padang City and students of Abdi Nusantara XIII FKH IPB. The purpose of this activity is to maintain the health of livestock residents through animal health services conducted routinely by the local service. As many as 16 local cows received health services. The observations showed that the prevalence of hard tick infestation in local cow in one of the Balai Gadang Village farmers reached 100%. Ticks are obligate bloodsucking ectoparasites in vertebrates, especially mammals, birds and reptiles throughout the world. Reportedly consists of two families of ticks, namely Ixodidae (hard tick) and Argasidae (soft tick). Found three genera of hard ticks have been identified namely; the genus Boophilus is the tick most commonly followed by Rhipicephalus and Ambylomma. This study shows that there is an abundance of high tick populations in livestock areas. Thus, it is highly recommended to carry out a tick control strategy, the application of akarisida to reduce the tick population and to improve good enclosure management to overcome the tick problem. Keywords: abdi nusantara XIII, ambylomma, boophilus, rhipicephalus, Padang local cow

Co-Authors Aditya Dharmawan, Aditya Aditya Wahyu Nugraha Afifah, Amalia Aisjah Girinda Akhmad Endang Zainal Hasan Akterono D. Budiyati Allaily Allaily Altaff Hendry Alvin Jefry Amrozi Andriyanto . Anita Esfandiari Arif Yahya Ario Damar Aulia Dina Kristina Bayu Sandi Chintia Ayu Puspita Daniel Latief Andre Darsono Darsono Desdiani Diah Nugrahani Pristihadi Dian Maulia Utami Dian Pratiwi DIMAS ANDRIANTO Dinar Arifianto Djumali Mangunwidjaja Dwi Astuti E. Mulyati Effend Effend, E. Mulyati Eko Prasetyo Nugroho Eko Suryanto Ekowati Handharyani Elok Puspita Rini Elpita Tarigan Eny Nur Aisyah Evy Damayanthi Fachriyan Hasmi Pasaribu Feri Kusnandar Gani, Ainal Gatut Ashadi Handayu Untari Handayu Untari Hanif Alamudin Manshur Hapsari Mahatmi Hardika Hardika, Hardika Hasim Hasim Hasim Hasim Hasim Hasim Hasim Hasim, . Hirawan Setiadi Husnawati, . I Ketut Suada I Komang Gede Wiryawan I wayan Teguh Wibawan Idqan Fahmi Ike Agustiani IPB, DGB Iris Rengganis Ismah Atika Salmah Japanis Permatasari, Retno Jusman, Sri Widia Azraki Kustiariyah Tarman Lala M Kolopaking Liesbetini Haditjaroko Lily Natalia M Agus Setiadi M. Aman Yaman M. Misbah, M. Ma'mun Sarma Maharani Maharani Maningtyas, Rosyidamyani Twinsari Masdiana C. Padaga Mega Safithri Miko Afrian Mochamad Alfinanda Santriagung Mohammad Irham Bagus Santoso Mohammad Sadikin Muhammad Daud Muhammad Ihsan Muhammad Luthfi Rahman Muhammad Ridla Muhammad Zuhdi Badruzzaman Muhammad Zulkifli Muharam Saepulloh Mutholaah Nadine Hanifa Permana Nahrowi Nahrowi Nahrowi Nathasia, Novena Neni Nuryani Nina Herlina Noviasari, Santi Nurjati Chairani Siregar Nurliani Bermawie Nurliani Bermawie Nurrahman, Arif Oktariza, Wawan Olive Wisepti Pratama Ono Suparno Pribadi, Yohanes Kusuma Purwiyatno Hariyadi Putri C. Eyanoer Rachmat Nabib Rahayu WoroWiranti Rahminiwati, Min Rayendra, Raendi Restu Librani Retno Damayanti Soejoedono Risa Tiuria Samsuridjal Djauzi Sandi, Bayu Sangko Sayuti Nasution Sekar Winahyu Ariadini Shila Rahmafia Putri SILMI MARIYA Siti Zulaikhah Slamet Budijanto Sri Purwaningsih, Sri Sri Wahyuni Salam Sudradjat Sumiati Suradi Suria Darma Tarigan Syahruddin Said Syifa Khaerani Teo Qin Yan Titi Candra Sunarti Tomas Iriyanto Tri Isyani Tungga Dewi Trioso Purnawarman Tsulatsy Puspitasari Purnamiharja Ulfah Juniarti Siregar Unang Patriana Upik Kesumawati Hadi Utamimah, Sri Vetnizah Juniantito Win Satya Rudramurti Winahyu Ariadini, Sekar Wiranda G Piliang Wiwin Winarsih Wiyono, W. Yeni Kartika Sari, Yeni Kartika

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