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Edukasi Masyasrakat dalam Mengurangi Rabies Serta Peningkatan Kesehatan Ternak melalui Pemeriksaan Masal pada Lima UPT di Kabupaten Agam, Sumatera Barat Arif Yahya; Muhammad Ihsan; Alvin Jefry; Agus Setiyono
Jurnal Pusat Inovasi Masyarakat (PIM) Vol. 2 No. 2 (2020): April 2020
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (451.793 KB)

Abstract

Rabies is a deadly disease transmitted from animals to humans with more than 59,000 people recorded or almost 1 death every 9 minutes CDC (2017). The main source of transmitting rabies is dogs through bites and saliva which contain viruses. Rabies is a strategic infectious animal disease (PHMS) whose existence is prioritized to be controlled in Indonesia. West Sumatra Province is still an area that is not yet free of rabies with extraordinary events (KLB). Aside from rabies, the biggest challenge is the many diseases of Jembrana. Jembrana is a strategic disease with its main host being only Bali cattle (Bos sondaicus). Decreased immune system cells (B lymphocytes) will cause the development of bacteria in the body's organs or sekuder infection (Directorate of Animal Health 2015). Thus causing losses including livestock mortality, declining health and quality of livestock, disruption of activities involving cattle, to the loss of closure of the affected area. Implementation of activities in the form of socialization and education about rabies, rabies vaccination, and mass animal health checks. The results of the field show that the number of treatments for livestock is 931, HPR vaccination, 1,211 and 859 people have received socialization regarding the spread of rabies in 5 UPTs in Agam District. Education and assistance are given to the public about the importance of eradicating rabies. The spread of jembrana disease can be inhibited by using vaccines through mass treatment programs. Key words: education, livestock, rabies, vaccines
Hepatoprotective Activity of Propolis Trigona spp., Hibiscus sabdariffa, and Myrmeleon sp. in Rats Induced by Paracetamol Akhmad Endang Zainal Hasan; Agus Setiyono; Miko Afrian
Current Biochemistry Vol. 9 No. 1 (2022)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.9.1.4

Abstract

Negative effects on health will occur in excessive use of paracetamol. The main objective of this study was to determine the hepatoprotective activity of Propolis Trigona spp., Hibiscus sabdariffa, and Myrmeleon sp. in rats induced by paracetamol. The study was conducted on 36 Sprague Dawley rats with 6 rats in each group. Group 1 (normal control), group 2 (positive control, temulawak 42.86 mg/kg BW), group 3 (negative control), group 4 (infusion of Myrmeleon 10 mg/kg BW), group 5 (propolis extract 0.02 ml/kg BW), and group 6 (Hibiscus sabdariffa infusion 0.02 ml/kg BW). Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) concentrations were determined spectrophotometrically and microscopically for liver histopathology. The results showed that during three weeks of treatment with Propolis Trigona spp., Hibiscus sabdariffa, and Myrmeleon sp. in rats, this treatment could decrease ALT and AST activity as the positive control. The ALT activity values ​​of the three materials were 69.26, 51.00, 51.00, 43.67, U/I with AST values ​​of 110.29, 97.33, 91.00, 91.33 U/I. Based on Duncan's test, there were significant differences between all treatments with negative and positive control groups. Likewise, with the histopathological scoring results, the negative control was found to be significantly different from all treatments. Therefore, treatment with propolis Trigona spp., Hibiscus sabdariffa, and Myrmeleon sp. may have hepatoprotective benefit.
DETEKSI IMUNOHISTOKIMIA ANTIGEN Coxiella burnetii SEBAGAI PENYEBAB Q FEVER PADA SAPI Sangko Sayuti Nasution; Agus Setiyono; Ekowati Handharyani
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (401.745 KB) | DOI: 10.21157/j.ked.hewan.v9i2.2835

Abstract

Penelitian ini bertujuan mendeteksi keberadaan antigen Coxiella burnetii sebagai penyebab Q fever pada organ sapi yang dikumpulkan di rumah potong hewan (RPH) Kota Medan dan Kabupaten Deli Serdang. Pada penelitian ini telah dikumpulkan organ limpa, paru-paru, dan hati dari 162 ekor sapi. Sampel organ tersebut kemudian diperiksa secara imunohistokimia dengan metode streptavidin peroksidase untuk melihat keberadaan antigen Coxiella burnetii menggunakan antibodi poliklonal terhadap Coxiella burnetii. Hasil pemeriksaan imunohistokimia menunjukkan 62/162 (38,3%) sampel sapi imunoreaktif terhadap Coxiella burnetii. Berdasarkan asal pengambilan sampelnya, sebanyak 40/101 (39,6%) sampel sapi yang berasal dari RPH Kota Medan dan 22/61 (36,1%) sampel sapi yang berasal dari RPH di Kabupaten Deli Serdang menunjukkan hasil imunoreaktif. Dilihat dari jenis organnya, dari 162 sampel sapi, antigen Coxiella burnetii dapat dideteksi pada 61 (37,7%) organ limpa, 12 (7,4%) organ paru-paru dan 2 (1,2%) organ hati. Hasil ini menunjukkan telah adanya infeksi Coxiella burnetii pada sapi di Kota Medan dan Kabupaten Deli Serdang.
GAMBARAN HISTOPATOLOGIS DAN KLINIS AYAM HERBAL SETELAH DIUJI TANTANG DENGAN VIRUS AVIAN INFLUENZA H5N1 Agus Setiyono; Nurliani Bermawie
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.485 KB) | DOI: 10.21157/j.ked.hewan.v8i1.1252

Abstract

Tujuan penelitian ini adalah mengetahui perubahan histopatologis dan klinis ayam herbal setelah ditantang dengan virus avian influenza (AI) H5N1. Seluruh ayam dibagi atas 3 kelompok perlakuan Kelompok kontrol, Kelompok I (I-1, I-2, dan I-3), dan Kelompok II (II-1, II-2, dan II-3). Masing-masing kelompok terdiri atas 15 ekor ayam sehingga jumlah seluruh ayam yang digunakan adalah 105 ekor. Ayam Kelompok I dan II masing-masing diberi herbal I (sambiloto, temu ireng, adas bintang, sirih merah) dan herbal II (sambiloto, adas bintang, sirih merah) selama 3 minggu sebelum ditantang virus. Ayam kelompok perlakuan secara keseluruhan tidak ada yang hidup hingga hari ke-8. Hasil penelitian menunjukkan bahwa 7 ekor ayam KII-3 (46,7%) masih hidup sampai hari ke-4 setelah uji tantang virus. Perubahan histopatologis sistem pernafasan ayam kelompok perlakuan menunjukkan pembendungan, edema, dan kerusakan sel epitel mukosa. Sistem limfoid juga menunjukkan pembendungan, deplesi folikel limfoid, dan fibrosis limpa dan bursa Fabrisius. Analisis imunohistokimia mengindikasikan partikel virus AI telah menyebar di organ atau jaringan sistem pernafasan dan sistem pertahanan.
PENGARUH PENGGUNAAN MODEL PEMBELAJARAN PROBLEM BASED LEARNING DAN MOTIVASI TERHADAP HASIL BELAJAR EKONOMI SISWA KELAS XI IIS MAN 1 LOMBOK BARAT Agus Setiyono
VALUTA Vol. 8 No. 2 (2022): Valuta
Publisher : UIR Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25299/valuta.2022.v8i2.10662

Abstract

This research is motivated by the low learning outcomes of students in several subjects. One of them is economics. One of the efforts that can be done to overcome these problems is the use of Problem Based Learning learning strategies in learning. The purpose of this research is to reveal the effect of problem based learning learning model and motivation on students' economic learning outcomes. The type of research used is Quasi Experiment using a 2x2 factorial design. The population in this study were students of class XI IIS-1 MAN 1 West Lombok, NTB for the 2021/2022 school year. The sample of this study was selected by simple random sampling technique with the results of students in class XI IIS-1 MAN 1 West Lombok as samples. The research data were obtained from the results of the economics study test and a learning motivation questionnaire. Data analysis was performed using the t-test and Two-Way ANOVA. The results showed that the economic learning outcomes of students who were taught using the problem based learning model were better than those of students who were taught using conventional learning. This also applies to students who have high and low motivation. Economic Learning Outcomes (cognitive, affective, and psychomotor) students who have high motivation who are taught by PBL learning model are better than students who have high motivation who are taught by conventional learning. The same thing is also shown in students who have low motivation and are taught with the PBL learning model looks better than students with low motivation who are taught by conventional learning.
PENGARUH PELATIHAN DAN DISIPLIN KERJA TERHADAP KINERJA KARYAWAN PADA PT BAHTERA ENERGI SAMUDRA TUAH JAKARTA SELATAN Setiyono, Agus; Japanis Permatasari, Retno
Journal of Research and Publication Innovation Vol 2 No 4 (2024): OCTOBER
Publisher : Journal of Research and Publication Innovation

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Abstract

This study aims to determine the simultaneous influence of training and work discipline on employee performance at PT Bahtera Energi Samudra Tuah South Jakarta. The method used is quantitative associative. The research population is 70 00 and all of them are sampled in this study. The data analysis techniques used are instrument test, classical assumption test, descriptive analysis, regression test, correlation coefficient test, determination coefficient test and hypothesis test. The results of the study showed that training had a significant effect on employee performance by 30.7% while the remaining 69.3% was influenced by other variables that were not studied. Work discipline generally affects employee performance by 17.1% while the remaining 82.9% is influenced by other variables that are not studied. Training and work discipline simultaneously affected employee performance by 37.8% while the remaining 62.2% were influenced by other variables that were not studied. The results of the study showed that the multiple liner regression equation Y = 14.617+0.423 X1+0.223 X2 means that employee performance even without the influence of training and work discipline already has an influence of 14.617. Simultaneously, training and work discipline have a positive and significant effect on employee performance, this is evidenced by the value of F calculation > F table, which is (20.364 > 3.13) with a significance of 0.000 < 0.05, then H0 is rejected and H3 is accepted, meaning that there is a positive and significant influence between training and work discipline on employee performance simultaneously at PT Bahtera Energi Samudra Tuah South Jakarta.  
Immunohistochemical Detection of Coxiella burnetii in Cattle Spleen Organs from Ampel Slaughterhouse, Boyolali Regency Nugroho, Eko Prasetyo; Setiyono, Agus; Hadi, Upik Kesumawati; Winarsih, Wiwin; Astuti, Dwi
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.48-55

Abstract

Q-fever is a zoonotic bacterial disease that caused by Coxiella burnetii. These microorganism are gram negative and obligate intracellular bacteria. This study was conducted to detect C. burnetii in cattle organs which collected from Ampel slaughterhouse Boyolali Regency. In this study, spleen, heart, lung, liver and kidney were collected from 100 cattle. The samples were tested by immunohistochemical (IHC) method using polyclonal anti- C. burnetii antibodies. Immunohistochemical examination found the presence of C. burnetii in the cytoplasm of macrophage cells with specific brown color only in the spleen as many as 4 out of 100 cattle showing immunoreactive (4%). The four positive individual samples were from Simental cattle. These results indicate that Q-fever was found in local cattle in Boyolali Regency.
Jati Diri Insan Akademik : Jati Diri Insan Akademik IPB, DGB; Sarma, Ma'mun; Damar, Ario; Sudradjat; Tarigan, Suria Darma; Setiyono, Agus; Sumiati; Siregar, Ulfah Juniarti; Hariyadi, Purwiyatno; Hasim; Kolopaking, Lala M.; Fahmi, Idqan; Oktariza, Wawan; Damayanthi, Evy; Setiadi, Mohamad Agus; Nahrowi; Sri Purwaningsih, Sri
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol 6 No 2 (2024): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0602.856-862

Abstract

Perguruan tinggi sebagai tempat insan akademik berkiprah saat ini mengalami berbagai tantangan dan ancaman yang dapat mengganggu fungsinya sebagai pusat pengembangan ilmu pengetahuan dan teknologi (iptek), serta menjadi penjaga keberlanjutan peradaban dan moralitas insan akademik agar tidak terdistorsi dan tererosi. Kemajuan teknologi (khususnya teknologi informasi), dekadensi moral global, tekanan ekonomi, orientasi praktis dan fokus pada pencapaian jangka pendek, perubahan paradigma pendidikan serta iklim ketidakpastian, menjadikan nilai-nilai tradisional perguruan tinggi terancam tergeser menjadi mesin penghasil tenaga kerja semata. Perguruan tinggi, yang seharusnya berperan sebagai benteng terakhir penjaga nilai-nilai akademik, serta sebagai agen perubahan (agent of change) yang berorientasi pada nilai kebaikan dan penjaga nilai moral (guardian of value), perlu jati diri insan akademik yang unggul, sehingga peran dan fungsi perguruan tinggi dapat tetap dipertahankan. Untuk memberikan pertimbangan dalam upaya menjaga tersebut, Policy Brief ini disusun melalui serangkaian proses diskusi anggota tim PAH Jati Diri Insan Akademik dan proses FGD yang melibatkan narasumber internal dan eksternal IPB. Policy Brief ini diakhiri dengan rekomendasi yang dapat diusulkan kepada penyelenggara pendidikan tinggi Indonesia agar fungsi perguruan tinggi dan insan akademik dapat tetap terjaga
Pengujian secara In Vitro Oligosakarida dari Ekstrak Tepung Buah Rumbia (Metroxylon sago Rottb.) sebagai Sumber Prebiotik Muhammad Daud; Wiranda G Piliang; Komang G Wiryawan; Agus Setiyono
Jurnal Agripet Vol 9, No 2 (2009): Volume 9, No. 2, Oktober 2009
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v9i2.627

Abstract

In vitro analysis of oligosaccharide from extract rumbia fruit (Metroxylon sago Rottb.) as prebioticABSTRACT. Despite a range of commercially available oligosaccharides there is plenty of room to develop new, functionally enhanced prebiotics. current generation of oligosaccharides was not rationally developed. better understanding of factors determining the prebiotic activity of a particular oligosaccharide. Despite the range of commercially available oligosaccharides mixtures (mainly fructo and galacto-oligosaccharides), very few studies are focused on the mechanisms behind the prebiotic activity of particular oligosaccharides. Probably this lack is due to the unavailability of well characterized oligosaccharide fractions for prebiotic function assessment. The objectives of this research were to asses the ability of lactic acid bacteria in fermentation of oligosaccharide and as prebiotic (in vitro). Material used was oligosaccharide of purified rumbia fruit extract. Analysis of oligosaccharide as prebiotic was conducted in vitro using lactic acid bacteria. The lactic acid bacteria consisted Bifidobacterium bifidum, Bifidobacterium animalis, Lactobacillus bulgaricus and Lactobacillus casei Rhamnosus. The growth media for bacteria was a liquid MRS basic medium where glucose was substituted by oligosaccharide of purified rumbia fruit extract. Incubation was in aerob for Lactobacillus and anaerob for Bifidobacterium in incubator 37oC. The lactic acid bacteria was calculated 24-48 hours during incubation periode. The variables observed were: oligosaccharide component, ability of lactic acid bacteria in fermentation of oligosaccharide, and growth of lactic acid bacteria (Lactobacillus and Bifidobacterium). The result showed that the oligosaccharide component from extract rumbia fruit consisted of: sucrose, stacchiose, and raffinose. The result showed that the oligosaccharide extract rumbia fruit was significantly (P0.05) the growth of lactic acid bacteria (Lactobacillus and Bifidobacterium) and fermentation of oligosaccharide. It is concluded that oligosaccharide of rumbia fruit extract could be used as prebiotic.
Penggunaan Prebiotik Oligosakarida Ekstrak Tepung Buah Rumbia (Metroxylon sago Rottb.) dalam Ransum terhadap Performan Ayam Pedaging Muhammad Daud; Wiranda G Piliang; Komang G Wiryawan; Agus Setiyono
Jurnal Agripet Vol 9, No 2 (2009): Volume 9, No. 2, Oktober 2009
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v9i2.624

Abstract

Effect of prebiotic oligosaccharide extract rumbia fruit (Metroxylon sago Rottb.) in the ration on broiler performanceABSTRACT. Prebiotic oligosaccharides are thought to provide beneficial effects in the gastrointestinal tract of humans and animals by stimulating growth of selected members of the intestinal microflora. Prebiotic oligosaccharides are defined as nondigestible food ingredients that provide beneficial effects to the host by stimulating the growth of selected microbial members of the gastrointestinal tract. Among the colonic bacteria capable of metabolizing prebiotic oligosaccharides and whose growth is stimulated are species of Lactobacillus and Bifidobacterium. Prebiotic oligosaccharides can be produced in transgli-cosylation reactions catalyzed by glycosidases. Glycosidases from different biological sources have specific ability to catalyze the formation of oligosaccharides with particular chain lengths (usually DP 7) and predominant glycosidic linkages. Oligosaccharide used this research was purified rumbia fruit extract as prebiotic for feed additive in the ration on broiler. The objectives of this research were to study the performance of broiler given of prebiotic oligosaccharide extract rumbia fruit (metroxylon sago Rottb.) in the ration. Two hundred day-old chicks of broiler were divided into three dietary treatments and four replications. Ration used was consisted of: R1 = basal ration (control), R2 = basal ration + 0,4% oligosaccharide extract rumbia fruit, and R3 = basal ration + 0,4% frukto-oligosaccharide (FOS). The variables observed were: feed consumption, body weight, feed conversion ratio, mortality, and production index. The result showed that the performances of the broiler supplemented prebiotic oligosaccharide extract rumbia fruit (R2) was significantly (P 0.05) differences between of feed consumption, body weight, and mortality. It is concluded that of prebiotic oligosaccharide extract rumbia fruit were able to decrease the mortality and feed consumption at six week of age.
Co-Authors Aditya Dharmawan, Aditya Aditya Wahyu Nugraha Afifah, Amalia Aisjah Girinda Akhmad Endang Zainal Hasan Akterono D. Budiyati Allaily Allaily Alvin Jefry Amrozi Andre, Daniel Latief Andriyanto . Anggraini , Ninik Anita Esfandiari Annasit Annasit, Annasit Arif Yahya Arifianto, Dinar Ario Damar Astrie Kusuma Dewi, Astrie Kusuma Aulia Dina Kristina Bayu Sandi Chintia Ayu Puspita Cleopas Martin Rumende Darsono Darsono Desdiani Diah Nugrahani Pristihadi Dian Pratiwi DIMAS ANDRIANTO Djumali Mangunwidjaja Dwi Astuti E. Mulyati Effend Effend, E. Mulyati Eko Prasetyo Nugroho Eko Suryanto Ekowati Handharyani Elok Puspita Rini Elpita Tarigan Eny Nur Aisyah Evy Damayanthi Fachriyan Hasmi Pasaribu Feri Kusnandar Gani, Ainal Gatut Ashadi Hamid, Farid Alfalaki Handayu Untari Handayu Untari Handoko, Susilo Hanif Alamudin Manshur Hapsari Mahatmi Hardika Hardika, Hardika Harry Isbagio Hasim Hasim Hasim Hasim Hasim Hasim Hasim Hasim, . Hendry, Altaff Husnawati, . I Ketut Suada I Komang Gede Wiryawan I wayan Teguh Wibawan Idqan Fahmi Iftitah, Selfi Lailiyatul Ike Agustiani Imron Arifin Indriani, Erdila IPB, DGB Iris Rengganis Ismah Atika Salmah Japanis Permatasari, Retno Jusman, Sri Widia Azraki Kustiariyah Tarman Lala M Kolopaking Liesbetini Haditjaroko Lily Natalia Lisnawati Rachmadi M Agus Setiadi M. Aman Yaman M. Misbah, M. Ma'mun Sarma Maharani Maharani, Maharani Maningtyas, Rosydamayani Twinsari Maningtyas, Rosyidamyani Twinsari Masdiana C. Padaga Mega Safithri Miko Afrian Mochamad Alfinanda Santriagung Mohammad Irham Bagus Santoso Mohammad Sadikin Muhammad Daud Muhammad Ihsan Muhammad Ridla Muhammad Zuhdi Badruzzaman Muhammad Zulkifli Muharam Saepulloh Mutholaah Nahrowi Nahrowi Nahrowi Nathasia, Novena Neni Nuryani Nina Herlina Nugroho, Budi Sulistiyo Nurjati Chairani Siregar Nurliani Bermawie Nurliani Bermawie Nurliani Bermawie Nurrahman, Arif Nyoman Kertia Oktariza, Wawan Olive Wisepti Pratama Ono Suparno Permana, Nadine Hanifa Pramono Prastyo, Danang Pribadi, Yohanes Kusuma Purnamiharja, Tsulatsy Puspitasari Purwiyatno Hariyadi Putri C. Eyanoer Rachmat Nabib Rahman, Muhammad Luthfi Rahminiwati, Min Rayendra, Raendi Restu Librani Retno Damayanti Soejoedono Rianto Setiabudy Risa Tiuria Rudramurti, Win Satya Samsuridjal Djauzi Sandi, Bayu Sangko Sayuti Nasution Sangko Sayuti Nasution, Sangko Santi Noviasari Sekar Winahyu Ariadini Setiadi, Hirawan Shila Rahmafia Putri SILMI MARIYA Siti Zulaikhah Slamet Budijanto Sri Purwaningsih, Sri Sri Wahyuni Salam Sudradjat Sumiati Suradi Suria Darma Tarigan Syahruddin Said Syifa Khaerani Titi Candra Sunarti Tomas Iriyanto Tri Isyani Tungga Dewi Trioso Purnawarman Ulfah Juniarti Siregar Unang Patriana Upik Kesumawati Hadi Utami, Dian Maulia Utamimah, Sri Vetnizah Juniantito Wibowo, Suryo Anggoro Kusumo Widiyanto, Totok Winahyu Ariadini, Sekar Wiranda G Piliang Wiwin Winarsih Wiyono, W. WoroWiranti, Rahayu Yan, Teo Qin Yeni Kartika Sari, Yeni Kartika