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Rhizosphere Bacteria Increased the Height and Root Number of Chili Plants (Capsicum annuum L.) Nofa Anjani; Triastuti Rahayu; Yasir Sidiq
Scientiae Educatia: Jurnal Pendidikan Sains Vol. 13 No. 1 (2024): June 2024
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24235/sc.educatia.v13i1.15158

Abstract

The need for potential bacteria as plant-promoting agents is still in high demand. Previous reports suggested five potential rhizobacteria isolated from the frangipani plant (Pulmeria accuminata) and producing indole acetic acid (IAA). However, evidence of its potential as a plant growth promoter has not been obtained. Therefore, this study aims to test the effect of rhizosphere bacterial inoculation on chili plants (Capsicum annuum L.). The method was a quantitative experimental method, with 15 repetitions for each treatment. The data were analyzed using a 95% student t-test confidence test or a significant level of 5% (a = 0.05). This inoculation begins with the germination of chili seeds until the radicula is observed. The germinated chili seedlings were soaked in bacterial suspension for an hour at room temperature. The results showed that inoculation using P38 isolate incubated for 6 hours significantly increased plant height and number of roots. The P31 isolate that was incubated for 6 hours inhibited the height of plants and the number of leaves of chili plants. Thus, these results provide important information about the effect of inoculation of newly isolated rhizosphere bacteria.
Diversity of endophytic bacteria in two banana cultivars and their potential for plant growth promoter TRIASTUTI RAHAYU; YEKTI ASIH PURWESTRI; SITI SUBANDIYAH; YASIR SIDIQ; DONNY WIDIANTO
Biodiversitas Journal of Biological Diversity Vol. 25 No. 7 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250704

Abstract

Abstract. Rahayu T, Purwestri YA, Subandiyah S, Sidiq Y, Widianto D. 2024. Diversity of endophytic bacteria in two banana cultivars and their potential for plant growth promoter. Biodiversitas 25: 2828-2838. The Klutuk banana plant (Musa balbisiana colla) is recognized as a resistant plant against biotic and abiotic stresses. The resistance trait of the cultivar is not exactly revealed but is most likely related to the presence of endophytic bacteria. This study aimed to determine the variation of banana cultivars and their organs on endophytic bacterial populations and their character as plant growth promoters (PGP). The study involved isolating endophytic bacteria from the root, corm, and petiole of cv. Klutuk and cv. Ambon, aged 4-5 months. Isolated endophytic bacteria were evaluated based on plant-growth-promoting (PGP) criteria. Subsequently, 16S rRNA sequences were amplified using 16S primers, 27f and 1492r, then sequenced. The obtained isolates were also identified by comparing the 16S sequences to the NCBI gene bank. The results showed that the population and number of isolates with the most PGP characters were in both banana cultivars' roots, petiole, and corm. Endophytic bacteria with multiple traits as PGP are composed of Phylum Proteobacteria (gamma, alpha, beta), Firmicutes, and Actinobacteria members. Several species are potential as they have multiple PGP traits with high-quantity test results, namely Microbacterium testaceum DSM 20166 strain, Simplicispira sp. CPCC 100842 strain, Klebsiella grimontii SB73 strain, Enterobacter mori LMG 25706 strain, Pantoea sp. CoA11 strain, Agrobacterium tumefaciens IAM 12048, Variovorax guangxiensis GXGD002 strain, Serratia nematodiphila DZ0503SBS1 strain, Bosea thiooxidans BI-42 strain, Erwinia tasmaniensis Et1/99 strain, Methylobacterium fujisawaense DSM 5686 strain, K2 isolate, K8 isolate, and K18 isolates. This study also revealed the endophytic bacterial population and character as plant organs influenced PGP. The roots and petioles became the banana plant organs colonized by endophytic bacteria with multiple traits as PGP. Overall, several isolates were discovered in cv. Klutuk were suspected of being new species with potential as PGP agents, and these were not identified in cv. Ambon.
Screening and identification of lipolytic potential of Actinobacteria from cemetery in Bonoloyo, Surakarta, Central Java, Indonesia ALDEN GANENDRA MADHAVA PRIYA HARDIANTO; TRIASTUTI RAHAYU; ERMA MUSBITA TYASTUTI; LINA AGUSTINA; NOOR ALIS SETIYADI; YASIR SIDIQ
Biodiversitas Journal of Biological Diversity Vol. 26 No. 6 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260623

Abstract

Abstract. Hardianto AGMP, Rahayu T, Tyastuti EM, Agustina L, Setiyadi NA, Sidiq Y. 2025. Screening and identification of lipolytic potential of Actinobacteria from cemetery in Bonoloyo, Surakarta, Central Java, Indonesia. Biodiversitas 26: 2786-2793. Cemetery soil is a unique reservoir of Actinobacteria with a significant lipolytic potential, in which lipids are 12.51-23.60% of the human body, a fact that has been largely overlooked in previous studies. However, studies on this subject are limited. This study, therefore, aimed to assess the lipolytic potential of 29 isolates from the Actinobacteria collection and to characterize the selected isolates further. Lipolytic screening was conducted on Tributyrin Agar (TBA) media for 72 hours, and the potential was determined by Lipolytic Index (LI). Subsequently, the colony morphology and spore type of the selected isolates were characterized through binocular microscopy and Scanning Electron Microscope (SEM). Identification was further conducted based on the 16S rRNA gene. These results showed that almost all tested isolates possess lipolytic ability, with the highest LI of 3.18 (T22). In general, the lipolytic potential of Actinobacteria was found to increase gradually after up to 72 hours of incubation. The aerial and vegetative mycelia were T5 for white and T22 for yellow, respectively, with closed spiral spores. Molecular identification showed that the T22 isolate is closely related to the Streptomyces sp. strain GSENDO-0580, with 99.93% similarity. In conclusion, this research underscores the urgent need for an in-depth study of Streptomyces sp. strain GSENDO-0580 is urgently needed, considering the extensive application of lipase enzymes in various fields.
Black Soldier Fly Bioconversion for Sustainable Organic Waste Management: A Systematic Review and Circular Economy Framework Hakim Garda Patria; Lista Ferlindia Putri Vanisa; Arundia Putri Wimala; Chintiya Fauziah Sulistiyawan; Rahima Laili Agustine; Faridhatun Nikmah; Yasir Sidiq; Siti Hadiyati Nur Hafida; Nour El Houda Alaoui; Dimitra Alexandra Nikolaou
Jurnal Penelitian Sains Teknologi Vol. 3, No 1, January 2027
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/saintek.v3i1.18035

Abstract

Organic waste is a major environmental challenge due to increasing generation and limitations in conventional waste management systems. This study systematically reviews the potential of Black Soldier Fly (BSF) (Hermetia illucens) bioconversion as an integrated approach to sustainable organic waste management. A qualitative systematic literature review was conducted through structured literature searching, screening, data extraction, and thematic synthesis using the PRISMA 2020 framework. The findings indicate that BSF larvae can rapidly reduce organic waste while producing protein-rich larval biomass and nutrient-rich frass. Bioconversion performance is influenced by substrate characteristics, temperature, moisture, aeration, larval density, and operational management. Beyond waste reduction, BSF technology supports resource recovery, alternative feed production, organic fertilizer generation, landfill diversion, and circular economy development. The main contribution of this review is an integrated conceptual framework linking BSF bioconversion with environmental benefits, economic value creation, and sustainable resource management. The findings demonstrate that BSF technology should be considered not merely as a waste treatment method but as an integrated strategy for sustainable organic waste management and circular economy implementation.
Co-Authors 'Azmi, Abidah Ulul A.A. Ketut Agung Cahyawan W Adilla Nur Azizah Adilla Nur Azizah Adityaradja, Bagas Alanindra Saputra ALDEN GANENDRA MADHAVA PRIYA HARDIANTO Anatri Desstya Anggita Juniar Laspartriana Anggun Dwi Nur Annisa Anhari, Minhatul Ulya Anisa, Rizka Sakinatun Anjani, Nofa Annur Indra Kusumadani Apriliana, Cindi Dila ARI SUSILOWATI Ariyanti, Olivia Puja Arum Dyah Ripdiyanti Arundia Putri Wimala Astuti, Dyah Auladiyah, Alfia Sa'adul Aurelia, Fanisha Azizah, Adilla Nur Bambang Harmanto, Bambang Bambang Sumardjoko Baskoro Adi Prayitno Baskoro Adi Prayitno Bowo Sugiharto Bowo Sugiharto Bowo Sugiyarto BUDI SETIADI DARYONO Chintiya Fauziah Sulistiyawan Condro Nur Alim D Djumadi Daryono, Budi S Dedi Gunawan Defina Anggita Silviani Devary Pradana Setyabudi Dewianty, Ratih Dicky Eka Prasetya Dimitra Alexandra Nikolaou Dito, Bahana Safiria DONNY WIDIANTO Donny Widianto Donny Widianto Dyah Astuti ERMA MUSBITA TYASTUTI Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Evi Dwi Kartikasari Fadilah, Fari Fari Fadilah Faridhatun Nikmah Febrianti, Nisa Febriyanti, Vika Gamal Abdul Nasir Zakaria Ganno Tribuana Aji Guntur Nurcahyanto Hakim Garda Patria Halimah, Syarifah Nur Haq, Muhammad Izzul Hardianto, Alden Ganendra Madhava Priya Hariyatmi Hartanto, Nadhifah Riski Harun Joko Prayitno Haryanto Haryanto Heri Siswanto Hernawan Sulistyanto Ihsan Nugroho Insani, Qori Tsaniyah Ainun Irfadhila, Dwega Izzatul Azmi Mulya Jakia, Nikmatu Jamil, Nazia Jumadi Jumadi Junita Dwi Wardhani Kistantia Elok Mumpuni Kistantia Elok Mumpuni Kun Harismah Laspartriana, Anggita Juniar Lina Agustina Lista Ferlindia Putri Vanisa Listy Hasti Mandiri Mandiri, Listy Hasti Maryana, Windri Masir, Rizkya A. Mauly Halwat Hikmat Mazwar Ismiyanto Miftakhul Huda Miftakhul Huda Minallah, Aldin Nasrun Muammar Fawwaz Muhammad Izzul Haq Muhammad Nizar Muhammad Noor Kholid Muhammad Samsuri Muhammad Syahriandi Adhantoro Muslimawati, Dinny Patria Nabila Fitriyani Nanang Mulyana, Nanang Naufal Ishartono Ningtyas, Yoga Dwi Windy Kusuma Ninik Nihayatul Wahibah Ninik Nihayatul Wahibah Nofa Anjani Noor Alis Setiyadi Nour El Houda Alaoui Nurhidayat Nurhidayat Nurul Hikmah Kartini Oktiatama, Firlana Bakti Puguh Karyanto Puguh Karyanto PUGUH KARYANTO Purnomo Purnomo Purnomo Purnomo Purwono , Bambang Putri Agustina Putri Agustina Putri Agustina Putri Agustina Putri Salwa Salsabilla Putri, Wiffy Zalina Rahayu, Triasuti Rahima Laili Agustine Ratih Dewianty Ratnasari Diah Utami Rika Dini Saputri Rima Munawaroh Risnasari, Wanda Datik Rizko Hadi Rizko Hadi, Rizko Rosyada, Alya Nugrahaeni Sabar Narimo Sabdina, Berlian Achya Putri Sajidan Santhyami Sari , Banuwati Kartika Seno, Hernandito Aryo Septinawati, Septinawati Setyabudi, Devary Pradana Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Silviani, Defina Anggita SITI SUBANDIYAH Siti Hadiyati Nur Hafida Siti Kartika Sari Siti Nur Syarifah Siti Nuraini Siti Nurjanah Sofyan Anif Sri Katoningsih Sri Sat Titi Hamranani Suparti Suparti Suparti Suparti Suryawati, Andari Tri Sutama Temon Bagus Hidayatullah Triastuti Rahayu Trio Ageng Prayitno Tyastuti , Erma Musbita Tyastuti, Erma Musbita Ulya, Nuriya Hazma Arifatul Vika Manda Putri Vina Listiawati Widi Widayat YEKTI ASIH PURWESTRI Yusnita Rahmawati Zainal Arifin Zakaria, Gamal Abdul Nasir Zulperi, Dzarifah