Article Per Year (5 Year)
p-Index From 2021 - 2026
6.062
P-Index
This Author published in this journals
All Journal Prosiding Seminar Biologi Bio-Pedagogi : Jurnal Pembelajaran Biologi AL KAUNIYAH IPTEK Journal of Proceedings Series Bioeksperimen: Jurnal Penelitian Biologi Pharmacon Scientiae Educatia: Jurnal Pendidikan Sains BIOEDUSCIENCE BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Journal of Education Technology Buletin KKN Pendidikan Jurnal Riset Biologi dan Aplikasinya Bioscientist : Jurnal Ilmiah Biologi Proceeding Biology Education Conference Jurnal Ilmiah Kampus Mengajar Abdi Teknoyasa Quagga: Jurnal Pendidikan dan Biologi International Conference on Education for All Bioeksperimen Journal of Biotropical Research and Nature Technology Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Sasambo: Jurnal Abdimas (Journal of Community Service) Proceeding of International Conference on Biology Education, Natural Science, and Technology Berita Biologi Indonesian Journal on Learning and Advanced Education (IJOLAE) BIOTROPIC
Yasir Sidiq
Universitas Muhammadiyah Surakarta
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

Published : 57 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 57 Documents
Search

 2   3   4   5   6 
Development of primers amplifying DNA barcoding genes matK and rbcL of Legundi (Vitex trifolia) Hartanto, Nadhifah Riski; Sari , Banuwati Kartika; Rahayu, Triasuti; Munawaroh , Rima; Fawwaz , Muammar; Purwono , Bambang; Zulperi, Dzarifah; Prayitno, Trio Ageng; Sidiq, Yasir
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.1497

Abstract

Legundi (Vitex trifolia) is one of the crucial ethno-pharmacological plants. However, the genetic exploration of these plants in Indonesia remains limited. Moreover, a set of primers can be an initial important step to explore the genetics of V. trifolia as well as to molecularly identify the species of Legundi. This study aimed to develop the two pairs of primers of maturase-K (matK) and ribulose bisphosphate carboxylase large subunit (rbcL) genes. This type of research is exploratory research using a quantitative approach. The research sampling are the leaves of the V. trifolia species, which were collected from Makassar, Indonesia, and the purposive sampling method. The data were obtained through DNA barcoding, and the data were analyzed using bioinformatics analysis. This study found two pairs of developed primers, matK and rbcL, successfully amplified both matK and rbcL target genes of V. trifolia. The newly developed species-specific primers successfully amplified the matK and rbcL genes of V. trifolia, and sequence analysis revealed high similarity values in BLAST and BOLD databases ranging from 99.2 to 100%, with PCR amplification of the matK marker showing particularly high DNA concentration and specificity for species-level identification. This study supports the genetic exploration and identification of useful ethno-pharmacy plants, V. trifolia. The conclusion of the study shows that two primer pairs of maturase-K (matK) and ribulose bisphosphate carboxylase large subunit (rbcL) genes have been developed from V. trifolia plants. This study supports the genetic exploration and identification of ethnopharmaceutical plants of V. trifolia and the role of bioinformatics tools in molecular studies of medicinal plants.
Penguatan Literasi dan Numerasi Siswa Sanggar Bimbingan Sungai Buloh Malaysia melalui Kegiatan Stimulan Profil Pelajar Pancasila Sidiq, Yasir; Ishartono, Naufal; Huda, Miftakhul; Rosyada, Alya Nugrahaeni; Anisa, Rizka Sakinatun; 'Azmi, Abidah Ulul; Auladiyah, Alfia Sa'adul; Mulyana, Nanang; Putri, Wiffy Zalina
Jurnal Ilmiah Kampus Mengajar Vol. 5, No. 2, Oktober 2025
Publisher : Asosiasi Lembaga Pendidikan Tenaga Kependidikan Perguruan Tinggi Muhammadiyah Aisyiyah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56972/jikm.v5i2.262

Abstract

Program pengabdian masyarakat melalui KKN Internasional ini dilaksanakan di Sanggar Bimbingan Sungai Buloh, Selangor, Malaysia, dengan tujuan untuk memperkuat literasi, numerasi, dan karakter siswa Warga Negara Indonesia (WNI) yang mengikuti pendidikan nonformal. Program berlangsung selama 28 hari dan melibatkan dosen serta mahasiswa Universitas Muhammadiyah Surakarta sebagai fasilitator. Pendekatan partisipatif digunakan agar siswa terlibat aktif dalam proses pembelajaran melalui kegiatan terintegrasi yang mengimplementasikan enam dimensi Profil Pelajar Pancasila. Data dikumpulkan melalui observasi, wawancara informal, dan dokumentasi, kemudian dianalisis menggunakan metode deskriptif kualitatif model Miles, Huberman, dan Saldaña. Hasil pengabdian menunjukkan bahwa kegiatan seperti Sanggar Sungai Buloh Sehat (S3), Aku Bangga Menjadi Anak Indonesia, dan Pekan Cinta Bahasa berkontribusi signifikan terhadap peningkatan literasi baca–tulis, literasi visual, dan numerasi sederhana siswa. Selain itu, terjadi penguatan karakter religius, gotong royong, mandiri, kreatif, berkebinekaan global, dan bernalar kritis dalam aktivitas pembelajaran. Kegiatan kebangsaan juga meningkatkan rasa nasionalisme dan identitas sebagai pelajar Indonesia di luar negeri. Meskipun terdapat tantangan berupa keterbatasan sarana dan variasi kemampuan akademik, inovasi pembelajaran yang dilakukan mahasiswa berhasil menciptakan atmosfer belajar yang lebih inklusif dan bermakna. Secara keseluruhan, program ini membuktikan bahwa implementasi Profil Pelajar Pancasila dapat efektif di lingkungan pendidikan nonformal melalui pendekatan kolaboratif dan kontekstual.
The Use of Natural Dyes from Beetroot Skin Extract (Beta Vulgaris) as Teaching Material on Cell Division for Senior High School Students Adilla Nur Azizah; Putri Agustina; Suparti Suparti; Alanindra Saputra; Yasir Sidiq
Indonesian Journal on Learning and Advanced Education (IJOLAE) Vol. 2, No. 1, January 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/ijolae.v2i1.9051

Abstract

One of the Basic Competencies (KD) in Biology for Indonesian Senior High School is KD 4.12 which reads "Presents observations of the process of mitosis in the onion root and determines the phases it finds". Based on the basic competency test, students are required to have skills in making and observing mitotic preparations of plant tissue with at least using onion. Observation of mitotic division in plant tissues requires skill in making preparations. Making preparations in observing cells and tissues of plants or animals needs staining. Dyes often used in lab work are synthetic dyes. One of the synthetic dyes commonly used is safranin. However, safranin is quite expensive. This limitation requires teachers to look for alternatives to synthetic dyes that are easily found at a more affordable price, namely with natural dyes. One of the plants that has the potential to overcome this issue is beetroot (Beta vulgaris). The purpose of this study was to determine the quality of plant tissue preparations (Allium cepa) stained with beetroot skin (Beta vulgaris) as a natural coloring agent. The research method used was an experimental method with a Completely Randomized Design consisting of two factors, namely the staining time (1 hour, 2 hours, and 3 hours) and the type of solvent (aquades and citric acid 10%). The results of the study were analyzed with qualitative descriptive methods which included contrast and clarity of the preparations. Based on the results of the study, the quality of Allium cepa root preparations using beetroot (Beta vulgaris) skin extract with variations in the type of solvent and duration of staining showed good results on the staining time of 1 hour and 2 hours. The staining time of the preparations can affect the contrast and clarity of the preparations.
Impact of Adaptive Educational Game Applications on Improving Student Learning: Efforts to Introduce Nusantara Culture in Indonesia Hernawan Sulistyanto; Djumadi Djumadi; Bambang Sumardjoko; Muhammad Izzul Haq; Gamal Abdul Nasir Zakaria; Sabar Narimo; Dyah Astuti; Muhammad Syahriandi Adhantoro; Devary Pradana Setyabudi; Yasir Sidiq; Naufal Ishartono
Indonesian Journal on Learning and Advanced Education (IJOLAE) Vol. 5, No. 3, September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/ijolae.v5i3.23004

Abstract

This study aimed to introduce Nusantara culture based on educational games by adjusting students' learning styles. Culture as an ancestral heritage tradition needs to be preserved by introducing it to the younger gen-eration from an early age. However, the survey results found that less than 26% of student respondents un-derstood Nusantara culture well. Compared to previous research, the model of cultural introduction through adaptive educational games is more fun because it is adapted to the way students learn. This research was carried out using the Design-Based Research (DBR) method through 4 stages of the procedure. The feasibil-ity test and application effectiveness test were carried out on a group of students from several elementary schools in Indonesia, who were taken using a cluster random sampling technique. The results of media design and content validation obtained an average value of 0.76 and 0.82, which means that the media is declared valid. The feasibility test used the System Usability Scale (SUS) with an average value of 80% in the acceptable category. The results of the research obtained a description of the comparison of the final scores of the control class and the experimental class, which was 55 compared to 75. This study concluded that learning media for introducing Indonesian culture based on adaptive educational games had a positive impact by effectively increasing learning outcomes on students' understanding of Indonesian culture. Further development of this game application can be expanded in the application of animation in more depth.
Transformasi Plasmid GFP (Green Fluorescent Protein) ke Bakteri Endofit Potensial K2 dan K8 dari Pisang Klutuk (Musa accuminata colla) Vika Manda Putri; Yasir Sidiq; Triastuti Rahayu
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 7 No. 2 (2024): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v8i1.11737

Abstract

This study aims to determine the results of GFP (Green Fluorescent Protein) transformation into potential endophytic bacterial isolates K2 and K8 from Klutuk banana (Musa accuminata colla). The method used is exploratory with data analysis conducted qualitatively and descriptively through literature review. The results showed that GFP transformation into potential endophytic bacterial isolates K2 and K8 from Klutuk banana was successful, evidenced by the presence of green fluorescent signals under UV light. This transformation is also beneficial for tracking the colonization of endophytic bacteria within plant tissues. The GFP plasmid pYL101C-sfGFP used has a total size of 6682 bp and contains a gene resistant to Gentamicin (GMC). In conclusion, GFP transformation on potential endophytic bacterial isolates K2 and K8 was successfully carried out and provides significant benefits in tracking endophytic bacterial colonization. Keywords: Endophytic Bacteria, Gentamicin, GFP, Klutuk Banana, GFP Plasmid Transformation
Identification of Bryophytes in Jumog Waterfall, Karanganyar, Central Java, Indonesia Yasir Sidiq; Alanindra Saputra; Siti Kartika Sari
Biotropic : The Journal of Tropical Biology Vol. 3 No. 2 (2019): Biotropic, Volume 3 Nomor 2, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2019.3.2.79-85

Abstract

Indonesia, as a mega-biodiversity country, has a high diversity of bryophytes (Liverworts, Hornworts, and Mosses). In addition, a waterfall is one of the most optimum places for bryophytes to life. One of many waterfalls in Indonesia is Jumog Waterfall. It is located in Karanganyar Regency, Central Java. This study aimed to know the diversity of bryophytes in Jumog Waterfall, identify the species, and analyze the genetic relationship through the species based on morphological characters. The explorative method was used to collect the samples. Identification of species was done by comparing samples to the handbook of identification. The results were; (1) there were fifteen species that found in Jumog Waterfall; (2) The highest similarity among species was 90%. They were grouped in genus Fissidentales, Order Dicranales, and Order Hypnobryales. Then, The lowest similarity was 30.8%. They were between Funaria hygrometrica and Riccia fliutans. It distinguished liverwort, hornwort, and mosses
Morphological and genetic identifications of Spirulina based on 16S rRNA gene Dito, Bahana Safiria; Sajidan, Sajidan; Susilowati, Ari; Sidiq, Yasir
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.14879

Abstract

Spirulina is a microalga with spiral or linear trichome shapes that has potential in industrial and food sectors due to its rich nutritional content. Spirulina can live in various aquatic habitats with different environmental conditions. Differences in cultivation environments can affect the morphological characteristics of Arthrospira due to phenotypic plasticity, making morphology-based identification prone to errors. Therefore, molecular identification using the 16S rRNA gene for phylogenetic analysis is necessary. This study aims to identify and analyze the genetic relationships of Spirulina from freshwater and brackish water based on 16S rRNA gene sequences. Freshwater Spirulina samples were taken from Bogor (BGR) and Yogyakarta (MRP), while brackish water Spirulina was taken from Jepara (JPR). DNA extraction was performed using the Zymo Research Quick-DNA Fungal/Bacterial Miniprep Kit, amplification of the 16S rRNA gene was conducted through PCR, followed by DNA sequencing. Phylogenetic analysis was carried out using MEGA11 to construct the phylogenetic tree. The morphology of strains BGR and MRP showed straight and thin filaments, whereas JPR had spiral and relatively long filaments. Sequencing analysis results showed that strains JPR and MRP had the closest homology similarity to Arthrospira sp. IAQUASC-C0001 with percentages of 99.34% and 99.12%, respectively. Meanwhile, Spirulina from BGR had the closest homology similarity to Arthrospira fusiformis strain AICB 668 with a percentage of 99.78%. Phylogenetic analysis indicated that strains BGR, JPR, and MRP are more closely related to Arthrospira than to Limnospira.
Co-Authors 'Azmi, Abidah Ulul A.A. Ketut Agung Cahyawan W Adilla Nur Azizah Adilla Nur Azizah Adityaradja, Bagas Alanindra Saputra Alanindra Saputra Alanindra Saputra Alanindra Saputra Alanindra Saputra Anatri Desstya Anggita Juniar Laspartriana Anggun Dwi Nur Annisa Anhari, Minhatul Ulya Anisa, Rizka Sakinatun Anjani, Nofa Annur Indra Kusumadani Apriliana, Cindi Dila ARI SUSILOWATI Ariyanti, Olivia Puja Arum Dyah Ripdiyanti Astuti, Dyah Auladiyah, Alfia Sa'adul Aurelia, Fanisha Azizah, Adilla Nur Bambang Harmanto, Bambang Bambang Sumardjoko Baskoro Adi Prayitno Baskoro Adi Prayitno Bowo Sugiharto Bowo Sugiharto Bowo Sugiyarto BUDI SETIADI DARYONO Condro Nur Alim D Djumadi Daryono, Budi S Dedi Gunawan Defina Anggita Silviani Devary Pradana Setyabudi Dewianty, Ratih Dicky Eka Prasetya Dito, Bahana Safiria Donny Widianto Donny Widianto Dyah Astuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Evi Dwi Kartikasari Fadilah, Fari Fari Fadilah Febrianti, Nisa Febriyanti, Vika Gamal Abdul Nasir Zakaria Ganno Tribuana Aji Guntur Nurcahyanto Haq, Muhammad Izzul Hardianto, Alden Ganendra Madhava Priya Hariyatmi Hariyatmi Hartanto, Nadhifah Riski Harun Joko Prayitno Haryanto Haryanto Heri Siswanto Hernawan Sulistyanto Ihsan Nugroho Irfadhila, Dwega Izzatul Azmi Mulya Jakia, Nikmatu Jamil, Nazia Jumadi Jumadi Junita Dwi Wardhani Kistantia Elok Mumpuni Kistantia Elok Mumpuni Kun Harismah Listy Hasti Mandiri Mandiri, Listy Hasti Maryana, Windri Masir, Rizkya A. Mauly Halwat Hikmat Mazwar Ismiyanto Miftakhul Huda Miftakhul Huda Minallah, Aldin Nasrun Muammar Fawwaz Muhammad Izzul Haq Muhammad Nizar Muhammad Noor Kholid Muhammad Samsuri Muhammad Syahriandi Adhantoro Munawaroh , Rima Muslimawati, Dinny Patria Nabila Fitriyani Nanang Mulyana, Nanang Naufal Ishartono Naufal Ishartono Ningtyas, Yoga Dwi Windy Kusuma Ninik Nihayatul Wahibah Ninik Nihayatul Wahibah Nurhidayat Nurhidayat Nurul Hikmah Kartini Oktiatama, Firlana Bakti Puguh Karyanto Puguh Karyanto PUGUH KARYANTO Purnomo Purnomo Purnomo Purnomo Purwono , Bambang Putri Agustina Putri Agustina Putri Agustina Putri Agustina Putri Salwa Salsabilla Putri, Wiffy Zalina Rahayu, Triasuti Ratih Dewianty Ratnasari Diah Utami Rika Dini Saputri Risnasari, Wanda Datik Rizko Hadi Rizko Hadi, Rizko Rosyada, Alya Nugrahaeni Sabar Narimo Sabdina, Berlian Achya Putri Sajidan Sajidan Santhyami Santhyami Sari , Banuwati Kartika Seno, Hernandito Aryo Septinawati, Septinawati Setyabudi, Devary Pradana Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Silviani, Defina Anggita Siti Kartika Sari Siti Nur Syarifah Siti Nur Syarifah Siti Nuraini Siti Nurjanah Sofyan Anif Sri Katoningsih Sri Sat Titi Hamranani Suparti Suparti Suparti Suparti Suparti Suparti Suryawati, Andari Tri Sutama Syarifah, Siti Nur Temon Bagus Hidayatullah Triastuti Rahayu Trio Ageng Prayitno, Trio Ageng Tyastuti, Erma Musbita Ulya, Nuriya Hazma Arifatul Vika Manda Putri Widi Widayat Yusnita Rahmawati Zainal Arifin Zakaria, Gamal Abdul Nasir Zulperi, Dzarifah