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Endang Sulistyaningsih
Departemen Budidaya Pertanian, Fakultas Pertanian, Universitas Gadjah Mada
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Journal : Indonesian Journal of Biotechnology

 1 
Elimination of shallot bulb viruses through heat treatment Margo Sulistio; Endang Sulistyaningsih; Siti Subandiyah
Indonesian Journal of Biotechnology Vol 20, No 2 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (876.266 KB) | DOI: 10.22146/ijbiotech.24196

Abstract

Shallot (Allium cepa L. Aggregatum group) is usually cultivated vegetatively. As a result, viruses tend to accumulate within the host plants and spread to healthy plants every crop cycle, reducing yield and bulb quality. There are a very limited number of studies about the elimination of shallot viruses through heat treatment. The objective of this research was to eliminate shallot viruses through heat treatment to produce virus-free plantlets. The leaves of Biru Lancor with specifc visual virus symptoms were detected by Reverse Transcription–Polymerase Chain Reaction (RT-PCR). Then bulbs of Biru Lancor that were positively infected by viruses were used as materials for heat treatment. The treatments were a control (without treatment), electric treatment at 15 mA for 10 minutes, heat treatment in an incubator at 37°C for 4 weeks, heat treatment in a waterbath at 45°C for 60 minutes, and combination of heat treatment in an incubator at 37°C for 4 weeks and heat treatment in a waterbath at 45°C for 60 minutes. After being subjected to heat treatment, the pseudo stem were cultivated in the MS Medium + 1 mg/L BAP + 1 mg/L IBA.Virus detection by RT-PCR was conducted 28 days after planting using samples of leaves from each plantlet. The results of this research showed that the treatments of electric treatment at 15 mA for 10 minutes and combination of heat treatment in the incubator at 37°C for 4 weeks and heat treatment in the waterbath at 45°C for 60 minutes could suppress the incidence of Shallot latent virus (SLV) until 100%. Heat treatment might have an important role in the degradation of virus particles by boosting Virus-Induced Gene Silencing (VIGS) as plant responses to virus infection.
Co-Authors , Tjhia Lian Nie Adi Setiawan Adi, Pudja Pramana Kusuma Agung Wahyu Susilo Agus Budi Setiawan Agus Budi Setiawan, Agus Budi AH. Maftuh Hafidh Zuhdi Aini, Khurotul Amalia T Sakya Amalia Tetrani Sakya Amalia Tetrani Sakya Andin Puspita Andin Puspita Anto Rimbawanto Ari Setiyaningrum Arif Wibowo Arif Wibowo Arif Wibowo Arizal Nur Hardiansyah AYPBC Widyatmoko Ayu Ainullah Muryasani Azis Purwantoro Azis Purwantoro Aziz Purwantoro Aziz Purwantoro Aziz Purwantoro Bambang Hendro Sunaminto Bambang Hendro sunarminto Bambang Hendro Sunarminto Bambang Hendro Sunarminto Bambang Hendro Sunarminto Benito Heri Purwanto Benito Heru Purwanto BH Purwanto Budiastuti Kurniasih Budijarto, Agus Catur Wasonowati Christina Maya Indah Susilowati Damar Suryaningndari Dhimas Ikhsan Prakoso Diana Diana Didik Indradewa Didik Indradewa Didik Indradewa Didik Indradewa Didik Indradewa Didik Indradewa Dwi Hartati Dyah Rachmawati Lucitasari Eka Candra Wardani Eka Tarwaca Susila Putra Eka Tarwaca Susila Putra Eko Hanudin Elizani, Prahesti Elsi Kris Dayanti Sembiring Endang Dewi Murrinie Endang Pudjihartati Erna Sri Wibawanti ETIKA, TAKARIADINDA DIANA Fahjar Prisiska Fenti Effendi Harjono, Saebani Hartanti Hartiningsih, Tri Herni Shintiavira Herni Shintiavira Herni Shintiavira Hidayat Pujisiswanto Hidayat Pujisiswanto Ho, Shu-Hsun Hutabarat, Srey Mariati I Ketut Gede, Sukaadha Ika Rahmawati Ilmiah, Haviah Hafidhotul INDAH PERMANASARI Indarwati, Lisa Dwifani Irenius Dwinanto Bimo Islam, Zainul Joko Budi Santoso Nugroho Karsidi Karsidi Khusnul Khotimah Kori Yati Kori Yati Kori Yati Krisna Dharmayanti Kurniasih, Budiastuti Levi Nilawati, Levi Libria Widiastuti Luthfianti, Fanni Maemonah, Maemonah Margo Sulistio Maria Marina Herawati Miranti Dian Pertiwi Muhammad Anshar Muhammad Anshar Muhammad Syahri Nanda Dwi Hafri Nasrullah Nasrullah Nasrullah Nasrullah Nindy Sevirasari Nindy Sevirasari Nopen Simamora Nur, Ahmad Lidiono Nursyaifuloh " Pahriyani, Ani Pangalila, Tamara Setyowati Pangestuti, Retno Pramono, Mellinia Valentiningtyas Prapto Yudono Prapto Yudono Prapto Yudono Prapto Yudono Prapto Yudono Prapto Yudono Prisiska, Fahjar Prisiska, Fahjar Priyono Suryanto Purwoko, Agus Puspita, Andin Putri , Antonietha March Natasya Putri Wulandari Rachma, Izza Azkiya Rachmanto Bambang Wijoyo Rajiman Rajiman Rani Agustina Wulandari Rani Agustina Wulandari Rina Pratiwi Pudja I. A Rini Rosliani Rini Rosliani Rizqi Dyah Susilowati Rohimah Handayani Sri Lestari Rohlan Rogomulyo Rudi Hari Murti Rudi Hari Murti Rudi Hari Murti Rudi Hari Murti Rudi Hari Murti SATRIYAS ILYAS sayekti, lady Sayekti, Lady Laduni Silalahi, Engelbertha E. Siregar, Mirawati Siti Subandiyah Siti Zahara Sri Dadi Pangestuti Sri Suwartiningsih STEFANY DARSAN, STEFANY Stephen Harper Suci Handayani Sumiyati Tuhuteru Sunarya Raharja Suryanti, Suryanti Suwijiyo Pramono SUWIJIYO PRAMONO Suwijiyo Pramono Syamsul Arifin Sylvia Diana Purba TAMTAMA, ARGA SYA’BAN Tangguh Prakoso Taryono, Taryono Taufiq Hidayat Teguh Iman Santoso Tjanturi, Sehan Tohari Tohari Tohari Tohari Tohari Tohari Tri Joko Tri Joko Tri Joko Valentina Dwi Suci Handayani Wen Shai Hung Wibowo, dan Arif WIDYATAMA, YUSA PUTRA Wiji Safitri Wulandari, Rani Agustina Yosuke Tashiro Yudo Swasono