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Journal : Journal of Global Pharma Technology

High Expression F2-Isoprostan (F2-IsoP), High Sterol Regulatory Element Binding Protein-2 (SREBP-2) and Low 2-Methoxyestradiol (2-ME) On Placenta Tissue as a Risk Factor of Pre-Eclampsia Jaya Kusuma AAN; Suwiyoga K; Mantik AN; Sukrama DM
Journal of Global Pharma Technology Volume 09 Issue 09
Publisher : Journal of Global Pharma Technology

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Abstract

To date, pre-eclampsia (PE) still a problem of Maternal Fetal Medicine related to high incidence, maternal and neonatal morbidity and mortality. Pre-eclampsia is caused by pregnancy; however, the mechanism has not been established so it is still a disease of theories. This relates to differences in treatment, resulting in different ways of prevention and output of PE itself. Recently, the role of F2-IsoP ,SREBP-2 and 2-ME was suspected to be very important in the mechanism of the PE. Meanwhile, the placenta acts as a source regulatory protein production, so that the material of this study was taken from placental tissue. The study objective was to prove the high F2I-soP, high SREBP-2 and low 2-ME expression in the placenta as risk factors for PE Case control study has been conducted in the department of obstetrics and gynecology Sanglah Hospital with 62 samples in 2015. The case group consisted of 31 mothers with PE and control groups consisted of 31 non-PE mother. The study material is a placental tissue. F2-IsoP and SREBP-2 expression study was performed using immunohistochemistry and 2-ME with ELISA techniques in Pathobiology Laboratory Faculty of Veterinary Medicine Udayana University. Data were analyzed with chi square test and discriminant using SPSS. The statistical test results are presented in tabular form and narrative. In this study, it was found that high expression of F2-IsoP increased the risk of PE  4 times higher (OR = 4.44; 95% CI = 1.53 to 12.94; p = 0.005) ; high expression of SREBP-2 increased the risk of PE 8 times higher  (OR = 8.19, CI95% = 2,311 to 29.073; p = 0.001) and low expression of 2-ME increased the risk of PE 5 times higher (OR = 5.23; CI95% = 1.75 to 15.55; p = 0.002). On the discriminant test, we obtained contributing risk factor for the occurrence of PE were SREBP-2, F2Isop and 2-ME (p = 0.002) respectively. Conclusion, high F2-IsoP expression, high SREBP-2 expression and low 2-ME expression in placenta were risk factors for PE. The most dominant risk factor of PE mechanism was SREBP-2.Keywords: Pre-eclampsia, F2-Isoprostane, Sterol Regulatory Element Binding Protein-2, 2-Methoxyestradiol.
High Level of Uterine Endocervical Interleukin-8, Matrix Metalloproteinase-8, and Interleukin 1Β as a Risk Factor for Preterm Labor Suwardewa TGA; Suwiyoga K K; Mantik Astawa N; Sutirta Yasa IWP
Journal of Global Pharma Technology Vol. 9 Issue 7 : 2017
Publisher : Journal of Global Pharma Technology

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Abstract

The preterm birth is a birth that takes place on a gestational age between 20–36 weeks and six days. The preterm birth   is the leading cause of neonatal mortality and morbidity. Despite the current treatment procedures, the incidence of preterm birth has not changed in the last two decades. Incomplete understanding of the pathophysiological mechanisms underlying preterm labor is the major obstacle to prevent preterm birth. Recently, endocervical inflammation has been thought as a risk factor of a preterm labor. Several important substances such as endocervical IL-8, MMP-8, and IL-1β, has been considered to play a key role on preterm birth through cervical ripening. With those assumptions, the detection of a higher level of uterine endocervical IL-8, MMP-8, and IL-1β can be used to predict preterm labor. The design of this study is an observational case control study with a case group, which consisted of pregnant women with 20–36 weeks and six days of gestational age who had signs of labor, and the control group consisted of pregnant women with 20–36 weeks and six days of gestational age who had no signs of labor. The material used for this study is cervical mucous, and the enzyme-linked immunosorbent assay method was used to assess the level of IL-8, MMP-8, and IL-1β at laboratory of Veterinary Medicine of Udayana University Denpasar. In this study we used 48 samples, among which 24 were case group, and 24 as a control group. The mean age of the women, gestational age, and parity from both group are (27.04 vs 28.62 years old), (31.8 vs 29.50 weeks), and (0.96 vs 1.29) respectively, which are statistically homogenous (p > 0.05). Each level of interleukin-8, matrix metalloproteinase, and interleukin-1β suggests a risk factor of a preterm labor for: 35 times (OR = 35.00; CI 95% = 6.95–176.39; p = 0.001); 6.6 times (OR = 6.60; CI 95% = 1.25–34.95; p = 0.016), and 8.3 times (OR = 8.3; CI 95% = 2.15–32.3; p = 0.001), orderly. Among these cytokines, one that contributes most through a preterm birth is interleukin-8 (61%), followed by matrix metalloproteinase-8 (27%), and interleukin-1β (12%).Conclusion: Endocervical inflammation with high level of IL-8, MMP-8, and IL-1β is a risk factor for preterm labor. IL -8 gives the most contribution on preterm labor.Keywords: Preterm birth, Endocervical IL-8, MMP-8, and IL-1β.
Prevalence of Pregnant Women with Anemia in Sangkan Gunung Village, Karangasem Regency, Bali-Indonesia Luh Seri Ani; Ketut Suwiyoga
Journal of Global Pharma Technology Volume 09 Issue 03
Publisher : Journal of Global Pharma Technology

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Abstract

Pregnant women are one of the groups with the risk of having anemia. Some factors that are correlated to anemia is an infectious disease, bleeding, and low iron daily intake.These factors were related to the knowledge level of pregnant women and iron supplementation compliance. This is a cross-sectional study with descriptive methods, where 34 pregnant women were chosen by consecutive sampling method. Anemia was then measured by sahli method, and hemoglobin level < 11 g/dl were categories as anemia. The results were analyzed with univariate and bivariate analysis using SPSS 23. Anemia in pregnant women at Sangkan Gunung village, Sidemen sub-district is20,6%.  Most anemia incidents were found in 20-35 years old pregnant women, with low education level, work at the office, zero parity, and in afirst trimester. Anemia was not associated with chronic energy deficiency, knowledge level and iron supplementation compliance level (P>0,05). Anemia prevalence of pregnant women was relatively low, but the preventive effort must be taken to decrease therate of anemia prevalence.Keywords: Knowledge, Compliance, Iron tablets, Pregnant women, Anemia.
Co-Authors Anak A. A. W. P. Dewi Anak Agung Gede Duwira Hadi Santosa Anak Agung Ngurah Jaya Kusuma Analysa, Analysa and I G. Alit-Artha Anom Suardika Anwar, Meirisa Afifah Nurmalia Arthawan Arthawan Aryana, Made Bagus Dwi Astawa, I Made Mulya Budiana, Nyoman Budiana, Nyoman Gede Budiman, Ardelia Clara Candijaya, Gita Sirini Christyani, Fenyta Danny Aguswahyudi Darmayasa, Putu Bagus Desak Putu Oki Lestari Deviyanti, Gery Puspa Dewi, Anak A. A. W. P. E. Joewarini Evert Solomon Pangkahila Feliciano Pinto, Feliciano Ferry Santoso Fido Anggli, Fido Fransiskus C Raharja Gde Ari Putra, Orion Reffa Gede Fajar Manuaba, Ida Bagus Hadisubroto, Yona S. Hanny Aditanzil Hanny Aditanzil Harrista, Daniel Victor Heni Sunyoto I Dewa Made Sukrama I Gde Adnyana Sudibya I Gde Raka Widiana I Gde Sastra Winata I Gede Adnyana Sudibya, I Gede Adnyana I Gede M Putra I Gede Mahendra Adiguna Dira I Gede Mega Putra I Gede Putu Surya I Gusti Ayu Putri Kartika, I Gusti Ayu I Gusti Ayu Sri Mahendra Dewi I Gusti Putu Mayun Mayura I Ketut Labir I Ketut Labir I Ketut Surya Negara I Ketut Tunas I Made Darmayasa I Nyoman Gede Budiana I Nyoman H Sanjaya I NYOMAN MANTIK ASTAWA I Putu Kusuma Yudasmara, I Putu Kusuma I Wayan Artana Putra I Wayan Megadhana I Wayan Putu Sutirta Yasa I Wayan Suranadi I. F. W. Putra I. Lesmana I.W. Weta Ida Bagus Gde Fajar Manuaba Ida Bagus Putra Adnyana Ika Widi Astuti, Ika Widi Ines Kurniaty Hartono Intan Puspa Intizam, Marwa Humaira IPG Wardhiana IPG Wardhiana, IPG Janet Sumampouw Jessica Nathalia JOHANA SENSY LENI MANNA K Tangking Widarsa KADE YUDI SASPRIYANA Kadek Fajar Marta Kerans, Fransiscus Anthony Ketut S. Negara Kirana, Ni Wayan Prabasiwi Kusuma, I Komang Wira Ananta L.S. Ani Leonardo ., Leonardo Leony Lim Lidia Widianti, Lidia Luh Putu Iin Indrayani Maker Luh Seri Ani Made Bagus Dwi Aryana Mahendrata, Prayascita MANNA, JOHANA SENSY LENI Mantik AN Manuaba, Ida Bagus Gede Fajar Marta, Kadek Fajar Moestikaningsih . Mona Mariana Mona Mariana Muhammad Freddy Candra Sitepu Muliawan, Erlin Purnama N.W.A. Utami Nathalia, Jessica Negara, Ketut S. Ngurah Harry Wijaya Surya, I Gede Ni Ketut Karneli Ni Ketut Karneli Ni Ketut Supasti Dharmawan Ni Made Suciani Nyoman Bayu Mahendra Ongko, Eric Gradiyanto Pramana, Bagus Andika Pratama, I Gede Bayu Adi Prayudi, Pande Kadek Aditya Puspa, Intan Putra, I Gede M Putra, Ida Bagus Agung Widnyana Putri, Desak Agung Istri Padma Putu Adi Sujana Putra Putu Ayu Indrayathi Putu Doster Mahayasa R. Niruri Raharja, Fransiskus C Rasmaya Niruri Rasmaya Niruri Rasmaya Niruri Rastiti, Ni Putu Riasa, I Nyoman Putu Rini Noviyani Riza Firman Satria Sanjaya, I Nyoman H Santoso, Ferry Sari, Jayanti Purnama Satriawan, Nyoman Gede Dikawijaya Setiawan, William Alexander Stella Kawilarang Stella Kawilarang, Stella Sugianto Sugianto Sumampouw, Janet Surya, I Gede Ngurah Harry Wijaya Tjokorda Gde Agung Suwardewa Wenas, Yongki Widia, Made Yudha Ganesa Wikantyas William Alexander Setiawan Wiraputri, Anak Agung Ratih Kusumadewi Yona S. Hadisubroto Yongki Wenas Yosevangelika Hutabarat