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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Akuakultur Indonesia Jurnal Teknologi Perikanan dan Kelautan Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences Berkala Perikanan Terubuk BIOTROPIA - The Southeast Asian Journal of Tropical Biology Current Biochemistry Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Kelautan Tropis Jurnal Akuatika Indonesia Omni-Akuatika Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Marinade Jurnal Teknologi Pertanian Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan JURNAL PERIKANAN TROPIS Asian Journal of Aquatic Sciences Indonesian Journal of Applied Research (IJAR) COJ (Coastal and Ocean Journal) Barakuda 45 Bambu Laut: Jurnal Pengabdian Masyarakat

Kustiariyah Tarman

Departemen Teknologi Hasil Perairan, Fakultas Perikanan Dan Ilmu Kelautan Institut Pertanian Bogor, Jl. Agatis, Bogor 16680 Jawa Barat Telp. (0251) 8622909-8622907, Fax. (0251) 8622907

Author-ID : 401679

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Medicine & Pharmacology Social Sciences Veterinary

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AKTIVITAS ANTIBAKTERI PROTEIN KAPANG Xylaria psidii KT30 TERHADAP Escherichia coli DAN Bacillus subtilis [Antibacterial Activity of Protein Fungus Xylaria psidii KT30 Escherichia coli and Bacillus subtilis] Aris Munandar; A. Zaenal Mustopa; Kustiariyah Tarman; Tati Nurhayati
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 2 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Previous research shows that extracellular protein of an algicolous fungus Xylaria psidii KT30 inhibited Bacillus pumilus, Listeria sp., Salmonella typhi, Staphylacoccus aureus, and Pseudomonas sp. with an average clear zone diameter of 7 mm. To enhance the potent antibacterial activity of extracellular protein from Xylaria psidii KT30, the present research demonstrated fungal growth optimization and purification of its secreted extracellular protein. The fungal growth optimization was performed following NaCl concentration and harvest day variations. The protein was precipitated using ammonium sulphate at 60%-90% saturation range and was purified through gel chromatography filtrationusing Sephadex G-50, eluted with 30% aq methanol. The active fraction possesing antibacterial activity was then determined resulting supernatant, pellet, and protein fraction.The fungal was optimally obtained after 15 days cultivation using freshwater.The highest protein yield was 1.67%, resulted over 90% saturation. Fractions 11 and 12 were the most active against Escherichia coli dan Bacillus subtiliswith clear zone diameter of 8 mm.Three bands of those fractions were detected through SDS-PAGE analysis, revealing molecular weights of 23.42, 20.09, and 14.33 kDa.

Teripang sebagai Sumber Pangan dan Bioaktif . Kustiariyah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 10 No 1 (2007): Buletin Teknologi Hasil Perikanan
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Sea cucumber (Holothurians) is one of fisheries commodities which has a high economic value. Generally sea cucumber is traded in the dried form (beche-de-mer). Then these holothurians are consumed as dried gonads (konoko) or dried gut (konowata), either holothurians crispy. Bioactive of sea cucumber, commonly known as holothurin, has drawn attention because of its wide spectrum of biological effects namely antibacterial, antifungal, anticancer or cytotoxic, haemolytic and anti-inflammation.Keywords : anticancer, anti-inflammation, Holothuria, natural products, sea cucumber1)Staf

SINERGIS TAURIN LINTAH LAUT (Discodoris sp.) DAN TEMULAWAK (Curcuma xanthorriza Roxb.) DALAM SERBUK MINUMAN FUNGSIONAL R. Marwita Sari Putri; - - nurjanah; Kustiariyah - Tarman
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 16 No 1 (2013): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

AbstrakSumber daya perairan seperti lintah laut (Discodoris sp.) dapat dibuat menjadi minuman fungsional.Lintah laut telah dilaporkan memiliki sifat antioksidan dan mengandung taurin. Penelitian ini dilakukandalam 2 tahap: 1) persiapan bahan baku (Discodoris sp,) 2) formulasi produk minuman fungsional.Tujuan dari penelitian ini adalah: 1) untuk menentukan konsentrasi komposisi bahan baku denganmempertimbangkan efek sinergis dari taurin pada minuman fungsional, 2) untuk mengetahui pengaruhpreparasi pada serbuk minuman fungsional terhadap jumlah taurin. Tiga formula terbaik yang diterimasecara organoleptik yaitu formula T1 (Discodoris sp. 20%, jahe 40%, 20% curcuma, lemon 20%), T2 formula(Discodoris sp. 25%, jahe 40%, 15% curcuma, lemon 20%) dan T3 formula (Discodoris sp. 30%, jahe 40%,10% curcuma, lemon 20%).Kata kunci: efek sinergis, lintah laut, minuman fungsional, taurin

Extraction and Characterization of Collagen from Sea Cucumber Flesh A. Alhana; Pipih Suptijah; Kustiariyah Tarman
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 18 No 2 (2015): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Sea cucumber (Stichopus variegatus) is one of the Echinodermata phylum that growsalong Indonesian coastal. Sea cucumber is potential source of collagen. The purposes of thisresearch were to determine the optimal concentration of NaOH and CH3COOH solutionin collagen production and analyze the physicochemical characteristics of collagen fromS. variegatus. Yield of the collagen was 1.5% (based on wet weight basis), produced bypretreatment with NaOH 0,30%, hydrolysis with CH3COOH 0.10% and extracted usingdistilled water. Protein, moisture, and ash content of the collagen was 67.68%, 13.64%,and 4.15%, respectively. Collagen was extracted using distilled water at 45°C during 2hand still had triple helix structure ; pH 7.37 ; melting temperature 163.67°C and whiteness69.25%. The major amino acid content of collagen were glycine, alanine, proline andglutamic acid.Keywords: NaOH, collagen, Stichopus variegatus, FTIR.

Profile of amino acid, fatty acid, and mineral content of Tambelo (Bactronophorus sp.) from Kendari, Southeast Sulawesi Riviani Riviani; Sri Purwaningsih; Kustiariyah Tarman
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 19 No 1 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Public coastal had trust natural material as medicine became one of the most important things inthe discovery of medicine latest. Papua, Belitung, and Kendari coastal communities believed that tambelo(Bactronophorus sp.) can treat various kinds of diseases like lumbago, rheumatism, cough, flu, malaria, andimprove production of the breast milk, increase appetite, and vitality of man. It was important to know whatcourse content of tambelo. Tambelo could be examined amino acids, fatty acids, and mineral contents. Thehighest essential amino acids in tambelo were leucyne, lysine, and valin of 0.57%, 0.39%, 0.36%. The highestnon essential amino acid was alanin, glutamic acid, and aspartic acid of 1.24%, 1.09%, and 0.78%. Barrierof amino acid was histidine. Fatty acid total of tambelo was 29.52%, whereas the fatty acid compositionsconsist of 10.09% saturated fatty acid (SAFA) was 14.37% monounsaturated fatty acid (MUFA) was 5.06%polyunsaturated fatty acids (PUFAs). Among them, those occuring in the highest proportions were palmaticacid (4.49%), oleic acid (5.73%), palmitoleic acid (4.96%), and Aracidic acid (1.88%). Tambelo had n6/n3ratio of 1.84. Tambelo had mineral contents as Natrium of 1144000 mg/kg, calcium of 17000 mg/kg, Kaliumof 21000 mg/kg, magnesium of 13000 mg/kg, phosphor of 1900 mg/kg, cadmium < 0.24 mg/kg, and lead<1.25 mg/kg.Keyword : amino acids, Bactronophorus sp., fatty acids, mineral contents, tambelo

Characterization and Photoprotector Activity of Endophytic Fungal Pigments from Coastal Plant Sarang Semut (Hydnophytum formicarum) Mada Triandala Sibero; Kustiariyah Tarman; Novriyandi Hanif
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 19 No 1 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Endophytic fungus RS3 isolated from coastal plant sarang semut (Hydnophytum formicarum) produced extracellular black pigment. The aims of this research were to obtain the pigment, to characterize and to determine the photoprotector activity. This research was conducted into several steps, that were determination of the best precipitating agent, characterization using instrument and solubility analysis, and analysis of Sun Protection Factor (SPF). Results showed the pigment was precipitated using acid solvent with pH ≤ 2.5. Functional groups of pigment were hydroxyl, aromatic ring, phenol and amine. According to its characteristics, black pigment produced by RS3 isolate was proposed as melanin. The photoprotector analysis showed the SPF value was 11.33.

Toxicity Sub chronic Water Extract Meretrix meretrix Linnaeus In Vivo on Sprague dawley Rats Azwin Apriandi; Kustiariyah Tarman; Purwantiningsih Sugita
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 19 No 2 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Meretrix meretrix is one of the shells of sea water are widely utilized by people as food. This clamalso has many properties and benefits, so in this study tested the effect of the water extract of Meretrixmeretrix against blood chemistry profile Sprague Dawley rats with the method (OECD 413: 2009). Based onobservations obtained growth, feed intake, weight of liver and kidney in normal conditions. Levels of urea,creatinine, cholesterol between the control mice treated with A/0.1 and A/1 were not significantly different(p> 0.05) while the levels of bilirubin and albumin between control mice treated with A/0.1 and A/1 resultssignificantly different (p<0.05), but all blood chemistry parameters tested is still in the normal category.

Antioxidant and Atibacterial Activities of Nipah (Nypa fruticans) against Vibrio sp. Isolated From Mud Crab (Scylla sp.) Imra Imra; Kustiariyah Tarman; Desniar Desniar
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 19 No 3 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

AbstractNipah (Nypa fruticans) is the potential plant for source of active compound such as antioksidant and antibacterial substances. The plants are dispersed in Sumatera, Kalimantan, Sulawesi, Maluku and Papua Island. The aim of this research were determine the antioxidant and antibacterial activity from of nipah (fruit and leaf) that it extraction with methanol, and than determine toxicity and active compound contained in this extract. Diffusion agar and DPPH method were use for antibacterial and antioxsidant assay, respectively. Antioxsidant activity from nipah’s leaf extract was more effective (22,5 μg/mL) than nipah’s fruit extract (415 μg/mL). This activity to be classified to the strong antioxidant activity (IC50<50 μg/mL). The antibacterial activity from leaf extract was strong to inhibited Vibrio sp. with inhibition zone 8,75 mm. The crude extract of nipah’s leaf was toxic with toxicity value is 663,598 μg/mL. Flavonoids, steroids, tanin, saponin and phenol hidroquinon were the active compounds contained in the extract of nipah’s leaf.

Anticancer Activity from Active Fraction of Sea Cucumber Nurul Mutia Putram; Iriani Setyaningsih; Kustiariyah Tarman
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20 No 1 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Sea Cucumber Holothuria atra is one of marine organisms has been used as a new source of novel bioactive compounds. Many of them have been used as the lead compounds in discovery of new anticancer drugs. The objective of this study was to determine the active fractions of sea cucumber (H. atra) which have anticancer activity. H. atra was macerated using ethanol and the extract was freezedried using a freeze dryer. The crude extract was partitioned using n-hexane, ethyl acetate, and methanol-water (3:1:1:1). Cytotoxicity test was performed using HeLa (cervic cancer) cell line and MCF-7 (breast cancer) cell line based on the MTT assay. The crude extract of H. atra showed the best cytotoxic activity against HeLa cells (IC50 = 12.48 µg/mL) and MCF-7 cells (IC50 = 17.90 µg/mL). The toxicity tests showed the IC50 value of the n-hexane fraction, ethyl acetate fraction, and methanol-water fraction against HeLa cells HeLa (IC50 = 76.45 µg/mL; 77.95 µg/mL; 14.27 µg/mL) and MCF-7 cells (IC50 = 58.50 µg/mL; 59.59 µg/mL; 14.33 µg/mL).

Antibacterial and Antioxidant Activity of Green Algae Halimeda gracilis from Seribu Island District Abdul Basir; Kustiariyah Tarman; D. Desniar
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20 No 2 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Seaweeds have ecological functions as primary producers in marine waters. It also has an important economic value as a producer of hydrocolloids (alginate, agar and carrageenan) that is used in various industries of food and pharmaceuticals. This study aimed to determine the antibacterial and antioxidant activity of green algae Halimeda gracilis. The study was conducted in several stages, sample collection and preparation, extraction of bioactive compound, fractionation, antibacterial and antioxidant test, and phytochemical. Extraction was done by maceration method using methanol and concentrated by rotary evaporator. The methanol extracts of H. gracilis were tested against Staphylococcus aureus and Escherichia coli. Methanol extract of H. gracilis formed inhibition zone against the test bacteria with diameter of inhibition zone was 10 mm and 6 mm, respectively. After liquid-liquid partition (water: ethyl acetate), inhibition zone was only seen in the ethyl acetate fraction of H. gracilis with diameter of inhibition zone was 6 mm and 7.50±1.71 mm, respectively. Antioxidant test methanol extracts and ethyl acetate fractions of H. gracilis each show IC50 value of 290.49 ppm and 375.50 ppm. Phytochemical test showed methanol extract of H. gracilis contains phenols and steroids.

Co-Authors - - nurjanah A. Alhana A. Zaenal Mustopa Abdul Basir Aditya Wahyu Nugraha Afifah, Amalia Afriani, Afriani Agus Sabdono Agus Setiyono Agus Trianto Ahmad Fauzan Lubis Aini, Khusnul anak agung putu ayu puspita negara Anisa Gianti Zhafira Anita Ervina Apon Zaenal Mustopa Apon Zaenal Mustopa Apriandi, Azwin Arafah, Purnama Arivai Santosae, Zacky Aulia Andhikawati Bambang Riyanto Bugi Ratno Budiarto Bustami Ibrahim Büyükateş, Yeşim Desniar - - Dewanti, Erika Wahyu Dinamella Wahjuningrum dita aguistina barus Dwi Safitri Dwicesaria, Maheswari Alfira Efi Toding Tondok Efianda, Teuku Reza Ekowati Chasanah Endar Marraskuranto Fahri Sinulingga Hamida, Nurul Hana Nurullita Prestisia Hartoyo Notonegoro Hartoyo Notonegoro Hawis H Madduppa Heder Djamaludin HEDI INDRA JANUAR Hedi Indra Januar Hendra Susanto Hidayati, Aulia Imanniar Yuta Ellana Dittama Imra Imra Iriani Setyaningsih Irzal Effendi Isti'anah, Ismi Jae-Kwan Hwang Joko Santoso Juno, Bintang Diva Kartika, Hanin Bela Khairul, Fitriah La Ode Fitradiansyah Liesbetini Haditjaroko Mada Triandala Sibero Mada Triandala Sibero Mala Nurilmala Manguntungi, Baso Maulidiani, Maulidiani Mega Safithri Meydia Meydia Meydia Meydia Meydia Mita Gebriella Inthe Mohammad Rofiqi Muhammad Arief Budiman Muhammad Nursid Muhammad Nursid Muhammad Sujatmiko Munandar, Aris MUNTI YUHANA N. Nurjanah Nabila Ukhty Neni Widowati Nguyen, Tien-Thanh Novriyandi Hanif Nurazizah, Mirah Afiza Nurjanah Nurjanah Nurul Mutia Putram Nurzakiah Nurzakiah Nurzakiah Nurzakiah Ocky Karna Radjasa Ono Suparno Pari, Rizfi Faris Pipih Suptijah Pipih Suptijah Pipih Suptijah Pratiwi, Ghina Eka Prayoga Pannindriya Prayoga Pannindrya Purwantiningsih Sugita putri, R Marwita Sari PUTRIANA SARI SIRAIT Putu Kristiani Kalontong R. Marwita Sari Putri R. Marwita Sari Putri Rahadian Pratama Riviani Riviani Roni Nugraha safrina dyah hardiningtyas Sahara, Rita Santosae, Zacky Arivai Saputra, Rahadita Maura Amanda Sari Putri, R. Marwita Sibero, Mada Triandala Sihono Sihono Silitonga, Ria Gusharani Sofyana, Neng Tanty Sri Hariati Sri Novita Sagita Sri Purwaningsih Sugeng Heri Suseno Sugita, Purwantiningsi Sumandiarsa, I Ketut Supinah, Pipin Susanto, Hendra Swestri Utami Syafiqoh, Nur Syam, Nur Asmasari TATI NURHAYATI Taufik Indarmawan Tiara Fitri Erinanda Tiara Ulfa Bachtiarini Tiftazani, M. Haekal Trilaksini, Wini Uju Uju Uju Umar, Mutmainnah Agustiawan Vepryany Oktaviarty Vitriyanna Mutiara Yuhendri Wahyu Ramadhan Widiarsyah, Farel Widodo Widodo Wini - Trilaksani Wong, Chen Wai Woro Hastuti Setyantini Wulandari Wulandari Yanti Fajarwati Yogiara Yogiara Yulia Oktavia Yusro Nuri Fawzya Zafira Khoirunnisa

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