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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Akuakultur Indonesia Jurnal Teknologi Perikanan dan Kelautan Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences Berkala Perikanan Terubuk BIOTROPIA - The Southeast Asian Journal of Tropical Biology Current Biochemistry Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Kelautan Tropis Jurnal Akuatika Indonesia Omni-Akuatika Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Marinade Jurnal Teknologi Pertanian Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan JURNAL PERIKANAN TROPIS Asian Journal of Aquatic Sciences Indonesian Journal of Applied Research (IJAR) COJ (Coastal and Ocean Journal) Barakuda 45 Bambu Laut: Jurnal Pengabdian Masyarakat

Kustiariyah Tarman

Departemen Teknologi Hasil Perairan, Fakultas Perikanan Dan Ilmu Kelautan Institut Pertanian Bogor, Jl. Agatis, Bogor 16680 Jawa Barat Telp. (0251) 8622909-8622907, Fax. (0251) 8622907

Author-ID : 401679

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Medicine & Pharmacology Social Sciences Veterinary

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Antioxidant and Atibacterial Activities of Nipah (Nypa fruticans) against Vibrio sp. Isolated From Mud Crab (Scylla sp.) Imra Imra; Kustiariyah Tarman; Desniar Desniar
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 19 No 3 (2016): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

AbstractNipah (Nypa fruticans) is the potential plant for source of active compound such as antioksidant and antibacterial substances. The plants are dispersed in Sumatera, Kalimantan, Sulawesi, Maluku and Papua Island. The aim of this research were determine the antioxidant and antibacterial activity from of nipah (fruit and leaf) that it extraction with methanol, and than determine toxicity and active compound contained in this extract. Diffusion agar and DPPH method were use for antibacterial and antioxsidant assay, respectively. Antioxsidant activity from nipah’s leaf extract was more effective (22,5 μg/mL) than nipah’s fruit extract (415 μg/mL). This activity to be classified to the strong antioxidant activity (IC50<50 μg/mL). The antibacterial activity from leaf extract was strong to inhibited Vibrio sp. with inhibition zone 8,75 mm. The crude extract of nipah’s leaf was toxic with toxicity value is 663,598 μg/mL. Flavonoids, steroids, tanin, saponin and phenol hidroquinon were the active compounds contained in the extract of nipah’s leaf.

Anticancer Activity from Active Fraction of Sea Cucumber Nurul Mutia Putram; Iriani Setyaningsih; Kustiariyah Tarman
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20 No 1 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Sea Cucumber Holothuria atra is one of marine organisms has been used as a new source of novel bioactive compounds. Many of them have been used as the lead compounds in discovery of new anticancer drugs. The objective of this study was to determine the active fractions of sea cucumber (H. atra) which have anticancer activity. H. atra was macerated using ethanol and the extract was freezedried using a freeze dryer. The crude extract was partitioned using n-hexane, ethyl acetate, and methanol-water (3:1:1:1). Cytotoxicity test was performed using HeLa (cervic cancer) cell line and MCF-7 (breast cancer) cell line based on the MTT assay. The crude extract of H. atra showed the best cytotoxic activity against HeLa cells (IC50 = 12.48 µg/mL) and MCF-7 cells (IC50 = 17.90 µg/mL). The toxicity tests showed the IC50 value of the n-hexane fraction, ethyl acetate fraction, and methanol-water fraction against HeLa cells HeLa (IC50 = 76.45 µg/mL; 77.95 µg/mL; 14.27 µg/mL) and MCF-7 cells (IC50 = 58.50 µg/mL; 59.59 µg/mL; 14.33 µg/mL).

Antibacterial and Antioxidant Activity of Green Algae Halimeda gracilis from Seribu Island District Abdul Basir; Kustiariyah Tarman; D. Desniar
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20 No 2 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Seaweeds have ecological functions as primary producers in marine waters. It also has an important economic value as a producer of hydrocolloids (alginate, agar and carrageenan) that is used in various industries of food and pharmaceuticals. This study aimed to determine the antibacterial and antioxidant activity of green algae Halimeda gracilis. The study was conducted in several stages, sample collection and preparation, extraction of bioactive compound, fractionation, antibacterial and antioxidant test, and phytochemical. Extraction was done by maceration method using methanol and concentrated by rotary evaporator. The methanol extracts of H. gracilis were tested against Staphylococcus aureus and Escherichia coli. Methanol extract of H. gracilis formed inhibition zone against the test bacteria with diameter of inhibition zone was 10 mm and 6 mm, respectively. After liquid-liquid partition (water: ethyl acetate), inhibition zone was only seen in the ethyl acetate fraction of H. gracilis with diameter of inhibition zone was 6 mm and 7.50±1.71 mm, respectively. Antioxidant test methanol extracts and ethyl acetate fractions of H. gracilis each show IC50 value of 290.49 ppm and 375.50 ppm. Phytochemical test showed methanol extract of H. gracilis contains phenols and steroids.

KANDUNGAN KOMPONEN BIOAKTIF DAN AKTIVITAS ANTIMIKROB EKSTRAK BINTANG LAUT (Culcita schmideliana) Kustiariyah Tarman; Hana Nurullita Prestisia; Iriani Setyaningsih; Meydia Meydia; Yogiara Yogiara; Jae-Kwan Hwang
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 15 No 3 (2012): Jurnal Pengolahan Hasil Perikanan Indonesia 15 (3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Bintang laut Culcita schmideliana merupakan salah satu spesies dari kelas Asteroidea yang memiliki potensi sebagai antimikrob. Tujuan penelitian ini adalah untuk menentukan komponen bioaktif dan aktivitas antimikrob dari bintang laut C. schemideliana. Penelitian ini dilakukan melalui beberapa uji, yaitu uji komponen fitokimia, uji aktivitas antimikrob, dan fraksinasi dengan kromatografi lapis tipis, serta bioautografi. Hasil uji fitokimia secara kualitatif menunjukkan bahwa ekstrak metanol mengandung alkaloid, steroid, flavonoid, saponin, dan ninhidrin, sedangkan ekstrak n-heksan dan etil asetat mengandung steroid dan saponin. Aktivitas antimikrob terbesar ditunjukkan oleh ekstrak etil asetat dengan konsentrasi 2 mg yaitu sebesar 16 mm. Fraksinasi KLT diidentifikasi memiliki 7 bercak pada lampu sinar UV λ 254 nm dan UV λ 366 nm menghasilkan 1 bercak. Pengamatan bioautografi terhadap bakteri B. subtilis menghasilkan zona hambat sebesar 7 mm pada Rf = 0,02 dan pada Rf = 0,62 menghasilkan zona hambat sebesar 3 mm.

Produksi Pigmen dan Identifikasi Kapang Penghasilnya Menggunakan Pendekatan DNA Barcoding Mada Triandala Sibero; Kustiariyah Tarman; Ocky Karna Radjasa; Agus Sabdono; Agus Trianto; Tiara Ulfa Bachtiarini
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 21 No 1 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia 21(1)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Kapang endofit laut diketahui sebagai sumber berbagai senyawa bioaktif yang berguna bagi kesehatan. Penelitian yang telah dilakukan sebelumnya membuktikan bahwa kapang endofit isolat RS3 asal tanaman pesisir Hydnophytum formicarum asal Sorong menghasilkan pigmen hitam dengan potensi sebagai fotoprotektor namun pada penelitian tersebut tidak memiliki data mengenai produksi pigmen serta identifikasi kapang penghasilnya. Penelitian ini bertujuan untuk menentukan produksi pigmen hitam asal kapang RS3 dan identifikasi kapang RS3 melalui pendekatan DNA barcoding. Kapang RS3 dikultivasi selama 30 hari menggunakan sistem statis dan sistem shaking. DNA barcoding pada identifikasi kapangRS3 menggunakan primer universal ITS1 dan ITS4 untuk mengamplifikasi wilayah internal transcribed spacer (ITS) yang menjadi conserve region pada kingdom fungi. Hasil penelitian menunjukkan bahwa kapang RS3 yang dikultivasi menggunakan sistem shaking mulai memproduksi pigmen pada hari-6 dan mencapai produksi tertinggi pada hari ke-18 (2,42±0,03 mg/L) sedangkan pada kultivasi sistem statis rendemen pigmen terbesar dihasilkan pada hari ke-30 (2,88±0,21 mg/L). Berdasarkan hasil analsis ITS rDNA diketahui bahwa kapang endofit RS3 memiliki 99% kemiripan dengan Annulohypoxylon stygium. Kapang ini telah didaftarkan di GeneBank denga nomer akses MG605083.1.

Aktivitas Antibakteri Ekstrak Kapang Laut Nodulisporium sp. KT29 terhadap Vibrio harveyi Sri Hariati; Dinamella Wahjuningrum; Munti Yuhana; Kustiariyah Tarman; Irzal Effendi; Fazril Saputra
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 21 No 2 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia 21(2)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Nodulisporium sp. KT29 merupakan salah satu kapang endofit yang diisolasi dari alga merah Eucheuma edule, tidak bersifat toksik, dan bersifat antibakteri. Penelitian ini bertujuan mengevaluasi aktivitas antibakteri ekstrak kapang laut Nodulisporium sp. KT29 terhadap Vibrio harveyi. Penelitian dilakukan dengan beberapa tahapan yaitu kultivasi kapang, ekstraksi senyawa aktif, pengujian aktivitas antibakteri, kromatografi lapis tipis (KLT), bioautografi, serta pengamatan kerusakan sel bakteri Vibrio harveyi. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etil asetat dan dipekatkan dengan rotary evaporator. Ekstrak Nodulisporium sp. KT29 diuji terhadap bakteri Vibrio harveyi pada konsentrasi 0,125; 0,25; 0,5; 1 dan 2 mg/disc. Ekstrak Nodulisporium sp. KT29 membentuk zona hambat tertinggi pada konsentrasi 2 mg sebesar 45,33±0,71 mm. Hasil uji KLT ekstrak Nodulisporium sp. KT29 menunjukkan adanya 7 spot retardation factor (Rf) dengan tiga komponen warna yaitu cokelat, kuning dan ungu. Uji bioautografi menghasilkan 2 spot zona hambat yaitu 16 mm dan 13 mm pada Rf 0,94 dan 0,14. Pengamatan menggunakan SEM menunjukkan bahwa kerusakan pada morfologi sel Vibrio harveyi dengan pemberian ekstrak Nodulisporium sp. KT29 yang ditandai dengan lisis.

Potensi Kolagen Teripang Emas sebagai Inhibitor Tirosinase Mega Safithri; Iriani Setyaningsih; Kustiariyah Tarman; Vitriyanna Mutiara Yuhendri; Meydia Meydia
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 21 No 2 (2018): Jurnal Pengolahan Hasil Perikanan Indonesia 21(2)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Kolagen memiliki aktivitas antioksidan dan mampu menghambat aktivitas tirosinase pada prosesmelanogenesis, salah satu biota laut yang dapat dijadikan sumber kolagen alternatif adalah teripang.Penelitian ini bertujuan menentukan karakteristik kolagen dan aktivitas penghambatan enzim tirosinaseoleh kolagen teripang emas (Stichopus hermanii). Penghilangan protein non kolagen dilakukan dengancara perendaman daging teripang menggunakan NaOH 0,1 M selama 48 jam. Isolasi kolagen dilakukanmenggunakan asam asetat 0.5 M selama 48 jam. Analisis gugus fungsi kolagen dilakukan menggunakanFTIR dan analisis aktivitas tirosinase dilakukan menggunakan spektrofotometer. Kadar protein nonkolagen yang diperoleh yaitu 0,090 mg/mL dan kolagen teripang emas memiliki rendemen 0,66%. Kolagenteripang memiliki nilai pH 6,91, gugus fungsi khas amida A (3379,29 cm-1), amida B (2924,09 cm-1), amidaI (1681,93 cm-1), amida II (1568,13 cm-1), dan amida III (1269,16 cm-1). Aktivitas penghambatan tirosinasemenunjukkan bahwa kolagen teripang emas memiliki nilai IC50 5610 ppm. Kolagen teripang emas belummampu menghambat aktivitas tirosinase.

Anticancer Activity of Spirulina Cultivated in Walne and Organic Media Putriana Sari Sirait; Iriani Setyaningsih; Kustiariyah Tarman
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 22 No 1 (2019): Jurnal Pengolahan Hasil Perikanan
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Spirulina is Cyanobacteria containing active components which is potentially showing anticancer activity. The purposes of this study were to determine anticancer activity and selectivity of crude extractsof Spirulina cultured using Walne and organic media, and to detect the apoptosis. The stages of this study included cultivation and harvesting of Spirulina, active components extraction, anticancer test and apoptosisdetection. Anticancer activity was determined using MTT assay. The crude extracts of Spirulina from Walne and organic cultures contained active components of alkaloids, flavonoids, steroids, and saponins. Theseextracts were not toxic to normal breast cells (MCF-12a), but showed cytotoxic activity in breast cancer cells (MCF-7). The crude extract of Spirulina from Walne culture had IC50 value of 36.23 ppm and selectivity index 30.07, while the IC50 of organic culture was 117.78 ppm and selectivity index 7.17. Detection of apoptosis with Hoechst dye 33342 showed the apoptotic activity of Spirulina crude extract against MCF-7 cells.

Karakteristik fisikokimia kolagen larut asam dari kulit ikan parang-parang (Chirocentrus dorab) Mega Safithri; Kustiariyah Tarman; Pipih Suptijah; Neni Widowati
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 22 No 3 (2019): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Waste of parang parang fish (Chirocentrus dorab) skin can be used as a source of collagen. Collagen isolation can be done chemically by the Acid Soluble Collagen (ASC) method. The objective of this research was to isolate collagen with ASC method and characterize their physicochemical. Collagen isolation consisted of pretreatment and hydrolysis with acids. The pretreatment used NaOH 0.1 M for 12 hours, while hydrolysis used acetic acid 0.5 M. Pretreatment results indicated that the concentration of non-collagen protein was 0.1243 mg/mL, while the yield collagen was 2.61%. The collagen had the viscosity of 6.50 cP, the denaturation temperature of 4°C, the transition temperature of 77.30°C, and the melting temperature of 153.90°C. The obtained collagen also had pH of 6.25. The fourier transform infrared (FTIR) spectra analysis showed the collagen contained amide A (3425.58), B (2924.09), I (1647.21), II (1543.05), and III (1246.02) (cm-1). The collagen also contained glycine (26.69%), proline (12.24%) and alanine (9.51%).

Peredaman Radikal DPPH oleh Ekstrak Metanol Spirulina platensis dan Teripang Emas (Stichopus hermanii) : DPPH radical scavenging by extract methanol of Spirulina platensis and gold sea cucumber (Stichopus hermanii) mega safithri; Kustiariyah Tarman; Iriani Setyaningsih; Anisa Gianti Zhafira
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 23 No 3 (2020): Jurnal Pengolahan Hasil Perikanan Indonesia 23(3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v23i3.32509

Spirulina platensis and Herrmann's sea cucumber (Sticophus hermanii) are marine biota that known have many health benefits. One of the benefit is as an antioxidant. The antioxidant activity of S.hermanii dan S. platensis from another country has been studied, but the antioxidant activity of both materials from Indonesia and the mixture haven’t studied yet. The purpose of this study was to test the antioxidant activity and to identify bioactive compounds of methanol extraxt of S. hermanii from Flores, S. platensis from Jepara and the mixture using DPPH method. Measurement of antioxidant activity used DPPH radical as an oxidant. Identification of bioactive compounds used a qualitative phytochemical test. The results showed that methanol extract of S.hermanii, S. platensis and the mixtures have IC50 value of 2,696.09±329.28 ppm, 324.92±4.06 ppm and 2,445.80±164.18 ppm. The methanol extract of Herrmann's sea cucumber contains alkaloids, saponins and triterpenoid, meanwhile S. platensis methanol extract contains alkaloids, flavonoids and steroids. Based on tests, the methanol extract from Herrmann's sea cucumber, S. platensis and the mixtures had very weak antioxidant activity with IC50 values more than 200 ppm.

Co-Authors - - nurjanah A. Alhana A. Zaenal Mustopa Abdul Basir Aditya Wahyu Nugraha Afifah, Amalia Afriani, Afriani Agus Sabdono Agus Setiyono Agus Trianto Ahmad Fauzan Lubis Aini, Khusnul anak agung putu ayu puspita negara Anisa Gianti Zhafira Anita Ervina Apon Zaenal Mustopa Apon Zaenal Mustopa Apriandi, Azwin Arafah, Purnama Arivai Santosae, Zacky Aulia Andhikawati Bambang Riyanto Bugi Ratno Budiarto Bustami Ibrahim Büyükateş, Yeşim Desniar - - Dewanti, Erika Wahyu Dinamella Wahjuningrum dita aguistina barus Dwi Safitri Dwicesaria, Maheswari Alfira Efi Toding Tondok Efianda, Teuku Reza Ekowati Chasanah Endar Marraskuranto Fahri Sinulingga Hamida, Nurul Hana Nurullita Prestisia Hanin Bela Kartika Hartoyo Notonegoro Hartoyo Notonegoro Hawis H Madduppa Heder Djamaludin Hedi Indra Januar HEDI INDRA JANUAR Hendra Susanto Hidayati, Aulia Imanniar Yuta Ellana Dittama Imra Imra Iriani Setyaningsih Irzal Effendi Isti'anah, Ismi Jae-Kwan Hwang Joko Santoso Juno, Bintang Diva Khairul, Fitriah La Ode Fitradiansyah Liesbetini Haditjaroko Mada Triandala Sibero Mada Triandala Sibero Mala Nurilmala Manguntungi, Baso Maulidiani, Maulidiani Mega Safithri Meydia Meydia Meydia Meydia Meydia Mirah Afiza Nurazizah Mita Gebriella Inthe Mohammad Rofiqi Muhammad Arief Budiman Muhammad Nursid Muhammad Nursid Muhammad Sujatmiko Munandar, Aris MUNTI YUHANA N. Nurjanah Nabila Ukhty Neni Widowati Nguyen, Tien-Thanh Novriyandi Hanif Nur Syafiqoh Nurjanah Nurjanah Nurul Mutia Putram Nurzakiah Nurzakiah Nurzakiah Nurzakiah Ocky Karna Radjasa Ono Suparno Pari, Rizfi Faris Pipih Suptijah Pipih Suptijah Pipih Suptijah Pratiwi, Ghina Eka Prayoga Pannindriya Prayoga Pannindrya Purwantiningsih Sugita putri, R Marwita Sari PUTRIANA SARI SIRAIT Putu Kristiani Kalontong R. Marwita Sari Putri R. Marwita Sari Putri Rahadian Pratama Rita Sahara Riviani Riviani Rizfi Faris Pari Roni Nugraha safrina dyah hardiningtyas Santosae, Zacky Arivai Saputra, Rahadita Maura Amanda Sari Putri, R. Marwita Sihono Sihono Silitonga, Ria Gusharani Sofyana, Neng Tanty Sri Hariati Sri Novita Sagita Sri Purwaningsih Sugeng Heri Suseno Sugita, Purwantiningsi Sumandiarsa, I Ketut Supinah, Pipin Susanto, Hendra Swestri Utami Syam, Nur Asmasari TATI NURHAYATI Taufik Indarmawan Tiara Fitri Erinanda Tiara Ulfa Bachtiarini Tiftazani, M. Haekal Trilaksini, Wini Uju Uju Uju Uju Uju Umar, Mutmainnah Agustiawan Vepryany Oktaviarty Vitriyanna Mutiara Yuhendri Wahyu Ramadhan Wahyu Ramadhan Widiarsyah, Farel Widodo Widodo Wini - Trilaksani Wong, Chen Wai Woro Hastuti Setyantini Wulandari Wulandari Yanti Fajarwati Yogiara Yogiara Yulia Oktavia Yusro Nuri Fawzya Zafira Khoirunnisa

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