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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Akuakultur Indonesia Jurnal Teknologi Perikanan dan Kelautan Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences Berkala Perikanan Terubuk Current Biochemistry Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Kelautan Tropis Jurnal Akuatika Indonesia Omni-Akuatika Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Marinade Jurnal Teknologi Pertanian Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan JURNAL PERIKANAN TROPIS Asian Journal of Aquatic Sciences Indonesian Journal of Applied Research (IJAR) COJ (Coastal and Ocean Journal) Barakuda 45 Molekul: Jurnal Ilmiah Kimia Bambu Laut: Jurnal Pengabdian Masyarakat
Kustiariyah Tarman
Departemen Teknologi Hasil Perairan, Fakultas Perikanan Dan Ilmu Kelautan Institut Pertanian Bogor, Jl. Agatis, Bogor 16680 Jawa Barat Telp. (0251) 8622909-8622907, Fax. (0251) 8622907
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Bioprospecting Marine Endophytic Fungi from Buton Island: Antibacterial Activity and Cellulase Production for Sustainable Blue Economy Isti'anah, Ismi -; Tarman, Kustiariyah; Lee, Wen-Chien; Suseno, Sugeng Heri; Nugraha, Roni; Effendi, Irzal; Nurbayasari, Rodiah; Maulana, Riyan
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 20, No 3 (2025): December 2025
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.1024

Abstract

Marine-derived endophytic fungi represent an underexplored reservoir of biologically active metabolites and enzymes with industrial relevance. This study investigated the fungal diversity associated with seaweed, seagrass, and mangrove leaf tissues collected from Buton Island, Southeast Sulawesi, with particular emphasis on antibacterial properties and cellulolytic activity. Thirty-two fungal isolates were successfully recovered and characterized based on morphological features. Antibacterial assays against Vibrio harveyi showed that several isolates, notably WB 1-2, WB 6-2, and SM 27-2, produced clear inhibition zones ranging from 11 to 13 mm, demonstrating notable antibacterial efficacy. Among all isolates, Aspergillus terreus (WB 1-2) exhibited the highest cellulase activity and was therefore selected for further enzymatic evaluation. The crude cellulase displayed maximum activity at pH 4 and a temperature of 70 °C. Hydrolysis products were qualitatively analyzed using HPLC, confirming the presence of glucose. These results highlight the dual potential of marine endophytic fungi as sources of antibacterial agents and thermostable cellulases. This work lays the groundwork for future development of eco-friendly enzyme production and marine-based antimicrobial resources, supporting sustainable biotechnological innovation within the blue economy framework.
Pelatihan Pembuatan Yoghurt diperkaya Spirulina dan Karaginan untuk Mendukung Pengembangan Ekonomi Biru di Kabupaten Malang Tarman, Kustiariyah; Setyaningsih, Iriani; Santoso, Joko; Adrianto, Luky; Suseno, Sugeng Heri; Isnaini, Cahyuning; Adelia, Shelika; Santosa, Zacky A; Hardiningtyas, Safrina D
Bambu Laut: Jurnal Pengabdian Masyarakat VOLUME 3, NOMOR 1, APRIL 2026
Publisher : Fakultas Ilmu Kelautan dan Perikanan Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35911/bambulaut.v3i1.46431

Abstract

Ganggang laut makro atau rumput laut merupakan salah satu komoditas perikanan utama Indonesia. Rumput laut jenis Kappaphycus alvarezii sebagai penghasil karaginan telah dibudidayakan secara luas di perairan Indonesia. Mikroalga Spirulina yang dikenal sebagai superfood juga telah dibudidayakan secara massal. Namun demikian, penggunaan kedua bahan tersebut dalam produk yoghurt masih terbatas. Program pengabdian masyarakat ini bertujuan untuk melakukan pelatihan diversifikasi yoghurt lokal di Kabupaten Malang melalui fortifikasi Spirulina dan karaginan. Kabupaten Malang merupakan penghasil susu sapi terbesar di Jawa Timur, namun pengembangan produk turunannya masih terbatas. Kegiatan dilaksanakan melalui pelatihan teori dan praktik kepada 35 peserta yang berasal dari kelompok peternak sapi perah, penyuluh, guru SMK, dan staf Dinas Peternakan dan Kesehatan Hewan, Kabupaten Malang. Evaluasi dilakukan dengan pretest dan posttest yang menunjukkan peningkatan skor pemahaman dari 96.47% menjadi 99.50%. Fortifikasi Spirulina dan karaginan dapat menambah nilai gizi, meningkatkan viskositas, dan memberikan keunggulan organoleptik pada produk yoghurt. Produk ini berpotensi mendukung Program Makan Bergizi Gratis (MBG) serta menjadi komoditas ekonomi desa yang berkelanjutan dalam kerangka ekonomi biru, sehingga perlu dilakukan tindak lanjut oleh pemerintah daerah setempat.  
Isolation and Characterization of Cellulase Enzymes from Marine Endophytic Fungi Tarman, Kustiariyah; Hardiningtyas, Safrina Dyah; Mardiana, Rina; Isti'anah, Ismi; Salamah, Ella
Molekul Vol 21 No 1 (2026)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2026.21.1.9857

Abstract

ABSTRACT. Enzymes called cellulases can break down the β 1,4-glycosidic bonds in cellulose to produce glucose or simple sugars. In this study, marine endophytic fungi that were isolated from the seagrass Enhalus sp. were used to obtain cellulase enzymes, assess activity, and identify the ideal pH and temperature for cellulase enzymes. The research involved a number of steps for the isolation and characterization of cellulase enzymes from Enhalus sp. named EN marine fungi, including preparing the seaweed by soaking it in warm and room-temperature water, extracting the cellulase enzymes by cultivating the marine fungi on production media with pretreated seaweed, counting the total cellulase activity was assessed using the Mandels et al. (1976) technique, and purifying the cellulase enzymes by depositing it with ammonium sulfate at a saturation level of 30-80%. According to the findings, on day 9 with seaweed carbon as a source of warm water immersion, cellulase enzyme isolated from seagrass Enhalus sp. had the greatest crude extract activity of total cellulase enzyme activity (FPAse), amounting to 0.0276 U/mL. The cellulase enzyme precipitated at a 70% saturation level of ammonium sulfate with an FPAse activity of 0.0381 U/mL and a specific activity of 1.85 U/mg. At pH 5 and 50 °C, cellulase enzyme deposition in this investigation was at its best. Keywords: cellulase enzyme, extraction, Enhalus sp., seaweed
META-ANALYSIS ON THE EFFECT OF INGREDIENTS ON CHEMICAL QUALITY OF NORI-LIKE PRODUCT Zafira Khoirunnisa; Uju; Kustiariyah Tarman; Vepryany Oktaviarty; Meydia; Ramadhan, Wahyu
Coastal and Ocean Journal (COJ) Vol 6 No 2 (2022): COJ (Coastal and Ocean Journal)
Publisher : Pusat Kajian Sumberdaya Pesisir dan Lautan IPB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/coj.v6i2.44813

Abstract

Akulturasi budaya dari Negara Jepang memengaruhi minat masyarakat Indonesia terhadap berbagai jenis makanan. Nori merupakan makanan tradisional khas dari Jepang yang diminati oleh masyarakat Indonesia dan terbuat dari rumput laut merah (Rhodophyta) jenis Porphyra. Perairan Indonesia yang bersifat tropis menyebabkan keberadaan rumput laut Porphyra langka. Oleh karena itu, telah banyak diteliti pembuatan produk nori-like yang terbuat dari berbagai jenis rumput laut atau bahan lainnya sebagai substitusi Porphyra. Penelitian ini bertujuan mengetahui pengaruh bahan penyusun terhadap mutu produk nori-like dari segi kimiawi seperti kadar air, abu, protein, dan lemak. Tahapan meta-analisis diawali dengan perumusan pertanyaan penelitian, kemudian dilanjutkan dengan penentuan kriteria inklusi dan eksklusi. Database yang digunakan dalam pencarian data adalah Google Scholar, Science Direct, Springer, dan lainnya. Data yang diperoleh melalui database tersebut selanjutnya diseleksi berdasarkan judul dan abstrak penelitian. Data yang telah memenuhi kriteria diekstrak dan dinilai kualitasnya. Ekstraksi data mengikuti Preferred Reporting Items for Systematic Review and Meta-analysis (PRISMA). Data yang diperoleh selanjutnya diolah secara statistika dan dilakukan interpretasi. Standard mean difference dari 3 penelitian mengenai nori-like dengan bahan penyusun yang berbeda digunakan sebagai parameter meta-analisis. Hasil meta-analisis menunjukkan bahwa terdapat perbedaan mutu dari segi karakteristik kimia yang signifikan. Parameter yang berbeda signifikan, yaitu kadar abu (SMD: -15,285; 95% CI: -27,032 s.d. -3,539; p-value 0,011), dan kadar protein (SMD: 12,084; 95% CI: 1,988 s.d. 22,181; p-value 0,019)
Peningkatan Pengetahuan dan Keterampilan Warga Desa Babakan melalui Pelatihan Pembuatan Cookies Spirulina Iriani Setyaningsih; Kustiariyah Tarman; Safrina Dyah Hardiningtyas; Rizfi Faris Pari; Desniar; Uju Uju; Wahyu Ramadhan; Tati Nurhayati
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 1 (2024): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.10.1.76-84

Abstract

Cookies are dry cakes made with flour as the primary baking ingredient. Spirulina platensis was rich in protein, amino acids, glycoproteins, essential fatty acids, polysaccharides, and beta-carotene. Therefore, using sago flour combined with spirulina and seaweed in cookies can increase the nutritional value and produce gluten-free cakes. This training aims to increase knowledge about spirulina, packaging, and product marketing strategies and the skills of Babakan village residents by providing training on making spirulina cookies. This community service activity was conducted by counseling and training on making spirulina cookies in Babakan Village, Dramaga. Counseling activities were carried out to increase knowledge about the potential of spirulina, good packaging, and product marketing strategies, as well as training on making spirulina cookies to improve the skills of Babakan village residents. There were 18 training participants: Family Welfare Empowerment administrators and members. Pre-tests and post-tests were carried out before and after the training activities regarding the topic provided by the speakers. The achievements of the training activities were seen from an increase in post-test results, which was 60% answered correctly compared to only 40% for the pre-test. The evaluation results of the participant's acceptance of training topics and training speakers had an average of 58 to 69%, respectively, perfect. While the participants's assessment of the training facilities and consumption had an average of 50%, they were good.
Co-Authors - - nurjanah A. Alhana A. Zaenal Mustopa Abdul Basir Adelia, Shelika Aditya Wahyu Nugraha Afifah, Amalia Afriani, Afriani Agus Sabdono Agus Setiyono Agus Trianto Ahmad Fauzan Lubis Aini, Khusnul anak agung putu ayu puspita negara Anisa Gianti Zhafira Anita Ervina Apon Zaenal Mustopa Apon Zaenal Mustopa Apriandi, Azwin Arafah, Purnama Arivai Santosae, Zacky Aulia Andhikawati Bambang Riyanto Bugi Ratno Budiarto Bustami Ibrahim Büyükateş, Yeşim Cahyuning Isnaini, Cahyuning Desniar - - Dewanti, Erika Wahyu Dinamella Wahjuningrum dita aguistina barus Dwi Safitri Dwicesaria, Maheswari Alfira Efi Toding Tondok Efianda, Teuku Reza Ekowati Chasanah Ella Salamah Endar Marraskuranto Fahri Sinulingga Hamida, Nurul Hana Nurullita Prestisia Hardiningtyas, Safrina D Hartoyo Notonegoro Hartoyo Notonegoro Hawis H Madduppa Heder Djamaludin Hedi Indra Januar HEDI INDRA JANUAR Hendra Susanto Hidayati, Aulia Imanniar Yuta Ellana Dittama Imra Imra Iriani Setyaningsih Irzal Effendi Isti'anah, Ismi Isti'anah, Ismi - Jae-Kwan Hwang Joko Santoso Juno, Bintang Diva Kartika, Hanin Bela Khairul, Fitriah La Ode Fitradiansyah Lee, Wen-Chien Liesbetini Haditjaroko Luky Adrianto Mada Triandala Sibero Mada Triandala Sibero Mala Nurilmala Manguntungi, Baso Maulidiani, Maulidiani Mega Safithri Meydia Meydia Meydia Meydia Meydia Mita Gebriella Inthe Mohammad Rofiqi Muhammad Arief Budiman Muhammad Nursid Muhammad Nursid Muhammad Sujatmiko Munandar, Aris MUNTI YUHANA N. Nurjanah Nabila Ukhty Neni Widowati Nguyen, Tien-Thanh Novriyandi Hanif Nurazizah, Mirah Afiza Nurbayasari, Rodiah Nurjanah Nurjanah Nurul Mutia Putram Nurzakiah Nurzakiah Nurzakiah Nurzakiah Ocky Karna Radjasa Ono Suparno Pari, Rizfi Faris Pipih Suptijah Pipih Suptijah Pipih Suptijah Pratiwi, Ghina Eka Prayoga Pannindriya Prayoga Pannindrya Purwantiningsih Sugita putri, R Marwita Sari PUTRIANA SARI SIRAIT Putu Kristiani Kalontong R. Marwita Sari Putri R. Marwita Sari Putri Rahadian Pratama Rina Mardiana Riviani Riviani Riyan Maulana Rizfi Faris Pari Roni Nugraha safrina dyah hardiningtyas Santosa, Zacky A Santosae, Zacky Arivai Saputra, Rahadita Maura Amanda Sari Putri, R. Marwita Setyantini, Woro Hastuti Sihono Sihono Silitonga, Ria Gusharani Sofyana, Neng Tanty Sri Hariati Sri Novita Sagita Sri Purwaningsih Sugeng Heri Suseno Sugita, Purwantiningsi Sumandiarsa, I Ketut Supinah, Pipin Susanto, Hendra Swestri Utami Syam, Nur Asmasari TATI NURHAYATI Taufik Indarmawan Tiara Fitri Erinanda Tiara Ulfa Bachtiarini Tiftazani, M. Haekal Trilaksini, Wini Uju Uju Uju Uju Uju Umar, Mutmainnah Agustiawan Vepryany Oktaviarty Vitriyanna Mutiara Yuhendri Wahyu Ramadhan Wahyu Ramadhan Widiarsyah, Farel Widodo Widodo Wini - Trilaksani Wong, Chen Wai Wulandari Wulandari Yanti Fajarwati Yogiara Yogiara Yulia Oktavia Yusro Nuri Fawzya Zafira Khoirunnisa