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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Akuakultur Indonesia Jurnal Teknologi Perikanan dan Kelautan Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences Berkala Perikanan Terubuk BIOTROPIA - The Southeast Asian Journal of Tropical Biology Current Biochemistry Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Kelautan Tropis Jurnal Akuatika Indonesia Omni-Akuatika Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Marinade Jurnal Teknologi Pertanian Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan JURNAL PERIKANAN TROPIS Asian Journal of Aquatic Sciences Indonesian Journal of Applied Research (IJAR) COJ (Coastal and Ocean Journal) Barakuda 45 Bambu Laut: Jurnal Pengabdian Masyarakat
Kustiariyah Tarman
Departemen Teknologi Hasil Perairan, Fakultas Perikanan Dan Ilmu Kelautan Institut Pertanian Bogor, Jl. Agatis, Bogor 16680 Jawa Barat Telp. (0251) 8622909-8622907, Fax. (0251) 8622907
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Isolasi heparin dari jeroan ikan tuna (Thunnus sp.) : Isolation of heparin from tuna viscera (Thunnus sp.) Hardiningtyas, Safrina Dyah; Kartika, Hanin Bela; Tarman, Kustiariyah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 26 No. 3 (2023): Jurnal Pengolahan Hasil Perikanan Indonesia 26 (3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v26i3.46634

Abstract

Ikan tuna merupakan komoditas penting Indonesia dengan nilai produksi yang meningkat setiap tahun. Pengolahan ikan tuna menghasilkan limbah 50-70%. Jeroan merupakan limbah yang dapat dimanfaatkan untuk menghasilkan biomaterial baru, contohnya heparin. Tujuan penelitian adalah untuk mendapatkan komponen heparin dari beberapa bagian jeroan ikan tuna, yaitu hati, lambung, usus dan sekum pilorus. Ekstraksi heparin dilakukan dengan metode enzimatis menggunakan enzim papain. Ekstrak kasar heparin dipurifikasi lebih lanjut dengan fraksinasi aseton. Gugus fungsi heparin murni dianalisis menggunakan FTIR spektrofotometer dan kandungan heparin ditentukan menggunakan sulfate GAG assay. Hasil penelitian menunjukkan bahwa usus dan sekum pilorus menghasilkan rendemen ekstrak sebesar 0,66±0,06% dengan kandungan heparin 43%. Heparin murni dari jeroan ikan tuna memiliki gugus fungsi penciri senyawa heparin, yaitu karboksil, asetil, hidroksil, siklus ester, dan atom N tersulfasi. Penelitian ini menunjukkan bahwa jeroan ikan tuna dapat menjadi alternatif sumber heparin.
Eksplorasi aktivitas enzimatik dari fungi endofit laut serta aplikasinya untuk hidrolisis kitosan: Exploration of enzymatic activity of marine endophyte fungi and its application for chitosan hydrolysis Budiman, Muhammad Arief; Tarman, Kustiariyah; Hardiningtyas, Safrina Dyah; Nurazizah, Mirah Afiza
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 27 No. 11 (2024): Jurnal Pengolahan Hasil Perikanan Indonesia 27(11)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v27i11.58419

Abstract

Fungi endofit laut merupakan fungi yang berasosiasi dengan jaringan organisme laut, yaitu rumput laut, koral, lamun, bunga karang maupun sedimen di lingkungan laut. Fungi dapat memproduksi metabolit sekunder berupa senyawa enzim ekstraseluler, di antaranya selulase, amilase, pektinase, kitosanase, lipase, maupun protease. Penelitian ini bertujuan menentukan potensi isolat fungi endofit berdasarkan aktivitas kitosanolitik, selulolitik, dan proteolitik untuk mendegradasi kitosan. Isolat fungi endofit laut diisolasi dari jaringan rumput laut, lamun, dan mangrove dari perairan Sukabumi dan Kepulauan Buton, Indonesia. Fungi sebanyak 20 isolat dilakukan penapisan aktivitas enzimatik (kitosanolitik, selulolitik, dan proteolitik) dengan metode difusi agar untuk mengetahui diameter zona bening yang dihasilkan. Proses hidrolisis dilakukan dengan menginokulasi isolat ke media hidrolisis yang berisi koloidal kitosan selama 7 hari. Hasil penelitian menunjukkan 12 isolat memiliki aktivitas kitosanolitik, 8 isolat selulolitik, dan 10 isolat proteolitik. Lima isolat memiliki aktivitas terhadap semua aktivitas enzimatik yang diuji. Dua isolat dengan kode KVA dan BM48A memiliki diamater zona bening kitosanolitik tertinggi. Kedua isolat tersebut diidentifikasi melalui DNA Barcoding sebagai spesies dengan galur Trichoderma harzianum KTR3 dan Aspergillus sydowii KTR50. Rendemen hidrolisat kitosan yang dihidrolisis menggunakan isolat T. harzianum KTR3 dan A. sydowii KTR50 7,44% dan 6,74%. Nilai viskositas yang dihidrolisis menggunakan isolat T. harzianum KTR3 dan A. sydowii KTR50 berturut-turut 21,10 cP dan 9,26 cP serta nilai bobot molekul 9,06 kDa dan 4,47 kDa. Aktivitas enzimatik yang dihasilkan oleh isolat fungi endofit laut memiliki kemampuan dalam mendegradasi kitosan, hal ini dibuktikan dengan terjadinya penurunan nilai viskositas dan bobot molekul kitosan.
Isolation of Endophytic Fungi From the Coastal Plant Terong Pungo (Solanum sp.) and Its Antibacterial Activity Against Oral Pathogenic Bacteria Ukhty, Nabila; Tarman, Kustiariyah; Setyaningsih, Iriani
BIOTROPIA Vol. 24 No. 1 (2017): BIOTROPIA Vol. 24 No. 1 April 2017
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1687.552 KB) | DOI: 10.11598/btb.2017.24.1.453

Abstract

Coastal plant locally known as terong pungo (Solanum sp.) was used by Aceh community as traditional medicine for toothache. The objectives of this study were to isolate endophytic fungi from the leaves of terong pungo and determine the antibacterial activity of the fungi against bacteria causing dental caries and infection. Eight endophytic fungi were isolated. Antagonism test among the isolates was performed to select the most dominant fungus. TP6 was the selected fungus based on the antagonism assay. Crude extracts of the fungus were macerated from the culture broth using ethyl acetate. The strongest antibacterial activity of the extracts was obtained when the fungus was at 12 days of cultivation. The fungal crude extract was strongly active against Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa with inhibition zone diameter of 20, 21 and 23 mm, respectively.
Antibacterial Activity of Red Pigment Isolated From Coastal Endophytic Fungi Against Multi-Drug Resistant Bacteria Sibero, Mada Triandala; Sahara, Rita; Syafiqoh, Nur; Tarman, Kustiariyah
BIOTROPIA Vol. 24 No. 2 (2017): BIOTROPIA Vol. 24 No. 2 August 2017
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5168.756 KB) | DOI: 10.11598/btb.2017.24.2.725

Abstract

Multidrug-resistant (MDR) bacterial infections have become a serious problem for the last several decades. To solve this issue, the finding of new antibiotic candidates is an urgency. Natural pigment is known to have biological activity against pathogenic bacteria. Coastal fungi are an unexplored source of natural pigment to fight MDR bacteria. This research was aimed to isolate coastal endophytic fungi from smooth ant plant (Hydnophytum formicarum), to screen endophytic fungi that produce red pigment, to extract the red pigment, to determine antibacterial activity of the red pigment, and to identify the coastal endophytic fungi producing the red pigment. In this study, seven fungi were isolated as endophytic fungi from H. formicarum. There were three isolates that produced extracellular pigment, i.e., RS 1A produced red pigment, RS 3 produced black pigment, and RS 6A produced yellow pigment. Our study focused on the red pigment which was produced by endophytic fungus strain RS 1A. The yield of red pigment was 8.8657% (w/w). This study showed that red pigment had antibacterial activity against Escherichia coli, Acinetobacter baumannii, and Proteus mirabilis strain MDR. Based on molecular and morphological identification, the endophytic fungus strain RS 1A was identified as Aspergillus versicolor.
Co-Authors - - nurjanah A. Alhana A. Zaenal Mustopa Abdul Basir Aditya Wahyu Nugraha Afifah, Amalia Afriani, Afriani Agus Sabdono Agus Setiyono Agus Trianto Ahmad Fauzan Lubis Aini, Khusnul anak agung putu ayu puspita negara Anisa Gianti Zhafira Anita Ervina Apon Zaenal Mustopa Apon Zaenal Mustopa Apriandi, Azwin Arafah, Purnama Arivai Santosae, Zacky Aulia Andhikawati Bambang Riyanto Bugi Ratno Budiarto Bustami Ibrahim Büyükateş, Yeşim Desniar - - Dewanti, Erika Wahyu Dinamella Wahjuningrum dita aguistina barus Dwi Safitri Dwicesaria, Maheswari Alfira Efi Toding Tondok Efianda, Teuku Reza Ekowati Chasanah Endar Marraskuranto Fahri Sinulingga Hamida, Nurul Hana Nurullita Prestisia Hartoyo Notonegoro Hartoyo Notonegoro Hawis H Madduppa Heder Djamaludin Hedi Indra Januar HEDI INDRA JANUAR Hendra Susanto Hidayati, Aulia Imanniar Yuta Ellana Dittama Imra Imra Iriani Setyaningsih Irzal Effendi Isti'anah, Ismi Jae-Kwan Hwang Joko Santoso Juno, Bintang Diva Kartika, Hanin Bela Khairul, Fitriah La Ode Fitradiansyah Liesbetini Haditjaroko Mada Triandala Sibero Mada Triandala Sibero Mala Nurilmala Manguntungi, Baso Maulidiani, Maulidiani Mega Safithri Meydia Meydia Meydia Meydia Meydia Mita Gebriella Inthe Mohammad Rofiqi Muhammad Arief Budiman Muhammad Nursid Muhammad Nursid Muhammad Sujatmiko Munandar, Aris MUNTI YUHANA N. Nurjanah Nabila Ukhty Neni Widowati Nguyen, Tien-Thanh Novriyandi Hanif Nurazizah, Mirah Afiza Nurjanah Nurjanah Nurul Mutia Putram Nurzakiah Nurzakiah Nurzakiah Nurzakiah Ocky Karna Radjasa Ono Suparno Pari, Rizfi Faris Pipih Suptijah Pipih Suptijah Pipih Suptijah Pratiwi, Ghina Eka Prayoga Pannindriya Prayoga Pannindrya Purwantiningsih Sugita putri, R Marwita Sari PUTRIANA SARI SIRAIT Putu Kristiani Kalontong R. Marwita Sari Putri R. Marwita Sari Putri Rahadian Pratama Riviani Riviani Roni Nugraha safrina dyah hardiningtyas Sahara, Rita Santosae, Zacky Arivai Saputra, Rahadita Maura Amanda Sari Putri, R. Marwita Sibero, Mada Triandala Sihono Sihono Silitonga, Ria Gusharani Sofyana, Neng Tanty Sri Hariati Sri Novita Sagita Sri Purwaningsih Sugeng Heri Suseno Sugita, Purwantiningsi Sumandiarsa, I Ketut Supinah, Pipin Susanto, Hendra Swestri Utami Syafiqoh, Nur Syam, Nur Asmasari TATI NURHAYATI Taufik Indarmawan Tiara Fitri Erinanda Tiara Ulfa Bachtiarini Tiftazani, M. Haekal Trilaksini, Wini Uju Uju Uju Umar, Mutmainnah Agustiawan Vepryany Oktaviarty Vitriyanna Mutiara Yuhendri Wahyu Ramadhan Widiarsyah, Farel Widodo Widodo Wini - Trilaksani Wong, Chen Wai Woro Hastuti Setyantini Wulandari Wulandari Yanti Fajarwati Yogiara Yogiara Yulia Oktavia Yusro Nuri Fawzya Zafira Khoirunnisa