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Analisis In Silico Senyawa Aktif Sprirulina platensis sebagai Inhibitor Tirosinase: Fatty Acid Profiles of Tuna Loin (Thunnus albacares) Sprayed by Filtered Smoke during Frozen Storage Prayoga Pannindriya; Mega Safithri; Kustiariyah Tarman
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 24 No 1 (2021): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v24i1.33122

Abstract

Hiperpigmentasi merupakan kondisi ketika melanin diproduksi secara berlebih sehingga menimbulkan bercak-bercak pada kulit. Kondisi ini selain menyebabkan masalah estetika juga meningkatkan produksi reactive oxygen species pada kulit. Salah satu cara menangani hiperpigmentasi adalah melalui inhibisi enzim kunci sintesis melanin yaitu tirosinase. Spirulina platensis adalah mikroalga yang telah banyak digunakan sebagai suplemen maupun obat karena kandungan nutrisinya yang tinggi. Ekstrak S. platensis telah terbukti secara in vitro memiliki kemampuan menginhibisi enzim tirosinase. Penelitian ini bertujuan untuk mencari senyawa aktif pada S. platensis yang berpotensi sebagai inhibitor enzim tirosinase. Senyawa-senyawa aktif yang terdapat pada S. platensis ditelusuri melalui studi pustaka kemudian diseleksi berdasarkan afinitas, toksisitas, dan aturan Lipinski. Analisis penambatan molekuler dilakukan dengan senyawa yang terpilih terhadap enzim tirosinase dengan asam kojat sebagai ligan kontrol. Hasil penambatan molekuler menunjukkan bahwa kaempferol paling berpotensi sebagai inhibitor tirosinase karena memiliki ΔG paling negatif sehingga memiliki afinitas terbesar terhadap enzim tirosinase.
SENYAWA METABOLIT SEKUNDER KAPANG ENDOFIT TP6 DAN TPL2 YANG DIISOLASI DARI TUMBUHAN PESISIR TERONG PUNGO (Solanum sp.) Nabila Ukhty; Kustiariyah Tarman; Iriani Setyaningsih
JURNAL PERIKANAN TROPIS Vol 2, No 2 (2015)
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (43.112 KB) | DOI: 10.35308/jpt.v2i2.26

Abstract

Secondary metabolites, are produced naturally and serve survival functions for the organisms that producing them. Endophytic fungi is the one of microorganism has a role in producing the secondary metabolites. Endophytic fungi TP6 and TPL2 isolated from coastal plant terong pungo (Solanum sp.). Endophytic fungi TP6 and TPL2 have secondary metabolite, including alcaloid, flavonoid, phenol hidrocuinon, and terpenoid compounds.
Antibacterial activity of Stichopus hermanii and Stichopus variegatus methanol extract Hendra Susanto; Mega Safithri; Kustiariyah Tarman
Current Biochemistry Vol. 5 No. 2 (2018)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Since 500 years ago sea cucumber has been used as wound healing medicine. Sea cucumbers are thought to function as a cell growth factor and have the potential as an antibacterial agent. This study aims to determine whether the methanol extract of sea cucumber has antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa so that it can be used to help diabetic wound healing of people infected with the same bacteria. The extraction method used was maceration with methanol to dried frozen simplicia. The simplicia was obtained by freeze drying to liquid preparation of heated fresh sea cucumber. The antibacterial activity test was performed using well diffusion method. There were no any antibacterial activity found in the two methanol extracts of sea cucumber. Keywords: antibacterial agent, Stichopus hermanii, Stichopus variegatus
Antibacterial Activity of Ethanol Extract of Spirulina platensis Prayoga Pannindrya; Mega Safithri; Kustiariyah Tarman
Current Biochemistry Vol. 7 No. 2 (2020)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.7.1.1

Abstract

Spirulina is a microalgae that has been widely used as various supplements and medicines because of its high nutritional content. The need for new antibacterial sources to solve the problem of antibiotic resistance, makes the potential of Spirulina as an antibacterial agent necessary to be researched. This study aims to analyze the antibacterial activity of ethanol extract of Spirulina platensis obtained from the Indonesian region against Staphylococcus aureus and Escherichia coli. Spirulina samples were macerated in ethanol solvent in a ratio of 1:10 (w / v). The antibacterial test used was the disc diffusion method with clindamycin positive control. Antibacterial test results showed that S. platensis in this study did not have antibacterial activity against E. coli and S. aureus.
CHARACTERIZATION OF CRUDE CELLULASE OF SEAGRASS ENDOPHYTIC FUNGUS Yulia Oktavia; Aulia Andhikawati; Tati Nurhayati; Kustiariyah Tarman
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 6 No. 1 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.752 KB) | DOI: 10.29244/jitkt.v6i1.8642

Abstract

In this study, cellulase was produced by endophytic fungus isolated from seagrass. Substrate used for incubating the fungus was the waste of agar industry. The objectives of this study were to optimize cellulase production and to characterize the highest activity of fungal crude cellulase. In our previous study, the EN isolate (isolated from Enhalus sp.) showed the highest cellulolytic index. Therefore, in this research we focused on cellulase activity of the isolate. Cellulase activity was determined based on endoglucanase activity, total cellulase activity, and β-glucosidase activity. The highest activity was then used to determine cellulase activity in enzyme characterization. The fungus was cultured in different concentration of agar extraction algal wastes. The fungal culture was incubated for 3-21 days with 120 rpm orbital shaker. The results showed that endoglucanase activity was 0.019-0.031 U/mL, total cellulase activity was 0.007-0.013 U/mL, and β-glucosidase activity was 0.00012-0.00361 U/mL. The highest endoglucanase and total cellulase activity were obtained from the fungal culture after 9 days incubation, β-glucosidase was obtained from the fungal culture after 15 days incubation with 1.5% of algal waste as substrate. The optimum pH and temperature were determined as 4 and 60 oC, maximum reaction rate (Vmax) and Michaelis-Menten constant (Km) for endoglucanase activity was determined as 0.044 U/mL and 0.103% respectively. Keywords: algal waste, cellulase, endophytes, enzymes, seagrass
ISOLATION AND SCREENING OF ENDOPHYTIC MARINE FUNGI FOR CELLULASE PRODUCTION Aulia Andhikawati; Yulia Oktavia; Bustami Ibrahim; Kustiariyah Tarman
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 6 No. 1 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.895 KB) | DOI: 10.29244/jitkt.v6i1.8643

Abstract

Cellulolytic fungi are potentially useful in biotechnology. Decomposition of substrates containing cellulose can be applied in bioethanol and enzymes production. Fungi are one group of microorganisms that can decompose cellulase materials.  This study aimed to obtain potential cellulolytic fungi isolated from marine habitat. Screening of the fungi was performed using CBM media with different salinity. Six isolates were tested quantitatively for cellulolytic activity using congo red staining. Cellulolytic index of algicolous and manglicolous fungi were lower than those of EN isolate, Veronea sp. KT19, and sponge associate (SMH). Fungi cultured in freshwater and seawater media possessed higher cellulolytic index in compared to those of cultures in 3% NaCl medium. However, freshwater and seawater media showed no significant effect. Cellulolytic index of EN isolate, Veronaea sp. KT19, and SMH after six days of incubation were 1.357; 1.267, and 1.278, respectively. All six isolates potentially produced cellulase in all medium tested. Keywords: Cellulolytic, cellulose, endophytes, enzyme
UTILIZATION OF MARINE FUNGAL Nodulisporium sp. KT29 METABOLITES TO IMPROVE THE PRODUCTION PERFORMANCE OF MARINE CULTURE OF WHITE SHRIMP Fazril Saputra; Dinamella Wahjuningrum; Kustiariyah Tarman; Irzal Effendi
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 8 No. 2 (2016): Elektronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.569 KB) | DOI: 10.29244/jitkt.v8i2.15839

Abstract

This study aimed to determine the best dose of administration of marine fungal Nodulisporium sp. KT29 metabolites to improve the production performance of marine culture of white shrimp. The experimental animals used were white shrimp Litopenaeus vannamei post larvae 12, which were reared in the sea using floating net cages with a stocking density of 700 post larvae/net. Experimental design used was a completely randomized design consisting of 3 treatments with 3 replications; control without the administration of Nodulisporium sp. KT29 metabolites, dietary of feed containing Nodulisporium sp. KT29 metabolites at doses of 20 mL/kg of feed and 40 mL/kg of feed. The results showed that survival rate, absolute length growth, weight gain, daily growth rate and feed conversion ratio in treatment groups were better than the control (P<0.05). The administration of marine fungal Nodulisporium sp. KT29 metabolites at a dose of 20 mL/kg of feed could improve the production performance of white shrimp cultivated in the sea with survival rate, daily growth rate and feed conversion ratio: 66.61±6.94%, 20.18±0.39 %/day and 3.20±0.22, respectively.
AKTIVITAS ANTIOKSIDAN PADA FORMULA TABLET TERIPANG KELING (Holothuria atra) Ahmad Fauzan Lubis; Sri Purwaningsih; Kustiariyah Tarman
Berkala Perikanan Terubuk Vol 44, No 2 (2016): Juli 2016
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (394.002 KB) | DOI: 10.31258/terubuk.44.2.51 - 69

Abstract

This study about activity of antioxidant aimed to determine the chemical characterization of sandfish, sandfish meat and sandfish powder, determine the best formulation in manufacturing Holothuria atra tablet based on DEPKES RI standard and to determine the antioxidant activity and the active compounds that act as antioxidants in the extract of Holothuria atra tablets. The method used in the manufacture of tablets is the direct compression method using 6 formulations to treat the kind of powder substance that is the ingredient of sandfish are intact and substances from the meat of sandfish. Tablet chosen based on the standards of the Ministry of Health that includes the physical characteristics of the test tablet and shelf-life stability. Tablet rivet sandfishtested the antioxidant activity by the method of 2,2-diphenyl-1-picrylhydrazyl (DPPH). Tests to determine the active compounds that act as antioxidants with thin layer chromatography (TLC) and bioautography antioxidants and also to determine the functional groups using analysis of Fourier Transform Infrared (FTIR). The yield of sandfish powder is considerably low. Yield of sandfish powder are 10.26% and sandfish meat powder are 9,82%. The protein content of sandfish powder are 60,89% lower compared to sandfish meat powders of 63,57%. Formula elected from the sandfish tablet test based on physical characteristics and stability test of the shelf life of the tablet is the formula A2 with the composition of the powder formulation Holothuria atra (25%), Ac-Di-Sol (2%), L-HPC (2%), Aspartame (0.5%), menthol (0.2%), talk (1%), magnesium stearate (1%) and lactose: mannitol (67.8%) and has antioxidant activity with IC50 value of 97.22 ppm. TLC test and Bio-autography showed that the class of antioxidant compounds are steroid, and suspected compounds at FTIR test, that act as antioxidants compound has similarities to the sterol class.
ISOLATION OF ENDOPHYTIC FUNGI FROM THE COASTAL PLANT TERONG PUNGO (Solanum sp.) AND ITS ANTIBACTERIAL ACTIVITY AGAINST ORAL PATHOGENIC BACTERIA Nabila Ukhty; Kustiariyah Tarman; Iriani Setyaningsih
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 24 No. 1 (2017)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1687.552 KB) | DOI: 10.11598/btb.2017.24.1.453

Abstract

Coastal plant locally known as terong pungo (Solanum sp.) was used by Aceh community as traditional medicine for toothache. The objectives of this study were to isolate endophytic fungi from the leaves of terong pungo and determine the antibacterial activity of the fungi against bacteria causing dental caries and infection. Eight endophytic fungi were isolated. Antagonism test among the isolates was performed to select the most dominant fungus. TP6 was the selected fungus based on the antagonism assay. Crude extracts of the fungus were macerated from the culture broth using ethyl acetate. The strongest antibacterial activity of the extracts was obtained when the fungus was at 12 days of cultivation. The fungal crude extract was strongly active against Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa with inhibition zone diameter of 20, 21 and 23 mm, respectively.
ANTIBACTERIAL ACTIVITY OF RED PIGMENT ISOLATED FROM COASTAL ENDOPHYTIC FUNGI AGAINST MULTI-DRUG RESISTANT BACTERIA Mada Triandala Sibero; Rita Sahara; Nur Syafiqoh; Kustiariyah Tarman
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 24 No. 2 (2017)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5168.756 KB) | DOI: 10.11598/btb.2017.24.2.725

Abstract

Multidrug-resistant (MDR) bacteria infections become a serious problem for these several decades. To solve this issue, finding of new antibiotics candidate in an urgency. Natural pigment is known to has biological activity against pathogenic bacteria. Coastal fungi are unexplored source of natural pigment to fight MDR bacteria. This research was aimed to isolate coastal endophytic fungi from smooth ant plant (Hydophytum formicarum), to screen endophytic fungi which produce red pigment, to extract the red pigment, to determine antibacterial activity of the red pigment and to identify the coastal endophytic fungi producing the red pigment. In this study, 7 fungi were isolated as endophytic fungi from H. formicarum. There were 3 isolates which produced extracellular pigment i.e. RS 1A which produced red pigment, RS 3 produced black pigment and RS 6A produced yellow pigment.  Our study focused on red pigment which is produced by endophytic fungus strain RS 1A. The yield of red pigment was 8.8657% (w/w).  This study showed that red pigment had antibacterial activity against Escherichia coli, Acinetobacter baumannii and Proteus mirabilis strain MDR. Judging from molecular and morphological identification, the endophytic fungus strain RS 1A was identified as Aspergillus versicolor.
Co-Authors - - nurjanah A. Alhana A. Zaenal Mustopa Abdul Basir Aditya Wahyu Nugraha Afifah, Amalia Afriani, Afriani Agus Sabdono Agus Setiyono Agus Trianto Ahmad Fauzan Lubis Aini, Khusnul anak agung putu ayu puspita negara Anisa Gianti Zhafira Anita Ervina Apon Zaenal Mustopa Apon Zaenal Mustopa Apriandi, Azwin Arafah, Purnama Arivai Santosae, Zacky Aulia Andhikawati Bambang Riyanto Bugi Ratno Budiarto Bustami Ibrahim Büyükateş, Yeşim Desniar - - Dewanti, Erika Wahyu Dinamella Wahjuningrum dita aguistina barus Dwi Safitri Dwicesaria, Maheswari Alfira Efi Toding Tondok Efianda, Teuku Reza Ekowati Chasanah Endar Marraskuranto Fahri Sinulingga Hamida, Nurul Hana Nurullita Prestisia Hanin Bela Kartika Hartoyo Notonegoro Hartoyo Notonegoro Hawis H Madduppa Heder Djamaludin HEDI INDRA JANUAR Hedi Indra Januar Hendra Susanto Hidayati, Aulia Imanniar Yuta Ellana Dittama Imra Imra Iriani Setyaningsih Irzal Effendi Isti'anah, Ismi Jae-Kwan Hwang Joko Santoso Juno, Bintang Diva Khairul, Fitriah La Ode Fitradiansyah Liesbetini Haditjaroko Mada Triandala Sibero Mada Triandala Sibero Mala Nurilmala Manguntungi, Baso Maulidiani, Maulidiani Mega Safithri Meydia Meydia Meydia Meydia Meydia Mirah Afiza Nurazizah Mita Gebriella Inthe Mohammad Rofiqi Muhammad Arief Budiman Muhammad Nursid Muhammad Nursid Muhammad Sujatmiko Munandar, Aris MUNTI YUHANA N. Nurjanah Nabila Ukhty Neni Widowati Nguyen, Tien-Thanh Novriyandi Hanif Nur Syafiqoh Nurjanah Nurjanah Nurul Mutia Putram Nurzakiah Nurzakiah Nurzakiah Nurzakiah Ocky Karna Radjasa Ono Suparno Pari, Rizfi Faris Pipih Suptijah Pipih Suptijah Pipih Suptijah Pratiwi, Ghina Eka Prayoga Pannindriya Prayoga Pannindrya Purwantiningsih Sugita putri, R Marwita Sari PUTRIANA SARI SIRAIT Putu Kristiani Kalontong R. Marwita Sari Putri R. Marwita Sari Putri Rahadian Pratama Rita Sahara Riviani Riviani Rizfi Faris Pari Roni Nugraha safrina dyah hardiningtyas Santosae, Zacky Arivai Saputra, Rahadita Maura Amanda Sari Putri, R. Marwita Sihono Sihono Silitonga, Ria Gusharani Sofyana, Neng Tanty Sri Hariati Sri Novita Sagita Sri Purwaningsih Sugeng Heri Suseno Sugita, Purwantiningsi Sumandiarsa, I Ketut Supinah, Pipin Susanto, Hendra Swestri Utami Syam, Nur Asmasari TATI NURHAYATI Taufik Indarmawan Tiara Fitri Erinanda Tiara Ulfa Bachtiarini Tiftazani, M. Haekal Trilaksini, Wini Uju Uju Uju Uju Uju Umar, Mutmainnah Agustiawan Vepryany Oktaviarty Vitriyanna Mutiara Yuhendri Wahyu Ramadhan Wahyu Ramadhan Widiarsyah, Farel Widodo Widodo Wini - Trilaksani Wong, Chen Wai Woro Hastuti Setyantini Wulandari Wulandari Yanti Fajarwati Yogiara Yogiara Yulia Oktavia Yusro Nuri Fawzya Zafira Khoirunnisa