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Journal : halal science

 1 
Evaluation of Pork-Specific DNA Primers for Halal Authentication in Processed Meats Salsabila, Aisyah Izmi Hamida; Wahyudi, Didik
Halal Science Volume 1 Issue 1
Publisher : ETFLIN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/halal0101364

Abstract

Ensuring the authenticity of halal-labeled food products is a growing global concern, particularly due to recurring reports of adulteration with non-permissible components such as pork. Molecular techniques, especially polymerase chain reaction (PCR), offer a sensitive and specific means to detect pork DNA in processed meats, where traditional protein-based assays often fail due to denaturation. This study evaluates the sensitivity of five pork-specific mitochondrial DNA primers, ND5, Cytochrome B(1), Cytochrome B(2), PPA8, and Pork (F2/R1), for detecting Sus scrofa DNA in processed meat products using conventional PCR. Genomic DNA was extracted from corned pork and beef meatball samples and amplified using each primer under optimized conditions. Of the five primers tested, only PPA8, Pork (F2/R1), and Cytochrome B(2) produced clear, specific amplicons in pork samples, with no cross-reactivity observed in beef samples. PPA8 and Pork (F2/R1) demonstrated the strongest and most consistent amplification, suggesting superior sensitivity and reliability. ND5 and Cytochrome B(1) showed poor performance, indicating limited applicability in processed food matrices. These findings confirm the utility of selected primers in halal authentication and highlight the importance of empirical validation in primer selection. Future work should focus on expanding primer testing across diverse processed food types and incorporating quantitative PCR to establish detection thresholds.
Co-Authors A Gani, A A. Gani, A. Gani Afianti, Linda Dwi Afiati, Lely Agung, Rendyansah ahmad yani Aju Tjatur Nugroho Krisnaningsih Alfaruqi, Nuri Thobibatus Shofia Ali Murtadho Anak Agung Istri Sri Wiadnyani Andriani, Disa Anjarani, Ajeng Viona Putri Annisa, Nenden Nur Anwar, Muhamad Syaeful Apita, Desma Hemaliyah Ardani, Edvi Gracia Ardy Prian Nirwana, Ardy Prian Auliantika, Dinda Helsa Azzahro, Yusri Despa Baharudin Baharudin Baharudin Bambang Hermawan Bayyinatul Muchtaromah Budhi, Dono Setya Chairul Amriyah Deden Makbuloh Dian Puspita Sari Dwi Agustina Dwi Gusmalawati Endang Sutariningsih Soetarto Endang Widyastuti Ervin Medianti Fadhila, Zulfa Nur Faizatul Ummah Fakhrul Rozy, Muhammad Fatkurohmah, Diana Fatoni, Ilham Fauzan, Muhammad Chusni Fitriyah Fitriyah Gusmiati, Lutfiana Hasanah Hadiani, Dimas Pratidina Puriastuti Haritzah, Rendha Maulana Hasan, M. Nur Hidayatullah, Rafi Fuzan Ilmi, Zahrobatul Lil Indivia, Imey Tamara Isna Arofatun Nikmah, Isna Arofatun Juwanita, Agnes Kharisma, Viol Dhea Kusuma, Eka Wisnu Leondro, Henny Lia Hapsari M. Dimas Yudi Witjaksono Manshur, umar Mauludyah, Rohmatul Mimi Salmawati Mose, Windi Muhammad Mustofa Muhammad Saefi Mujahidin Ahmad Munandar, Hanozanor Fadli Nawang, Pungky Nisa, Kurnia Khoirun NURUL AZIZAH Oke Anandika Lestari Pratimasari, Diah Prihandini, Peni Wahyu PUDJI ASTUTI Punjungsari, Tyas Nyonita Purwandani, Safitri Putri, Sheli Feliyan Rachman, Rhesma Sylvia Radita, Novia Fitri Rahmawati, Valentina Indah Ramadanti, Isma Salsabila Rodiyati Azrianingsih Ruri Siti Resmisari Rusdi, Nurhidaya Salsabila, Aisyah Izmi Hamida Saputra, Dedech Muhammad Sari, Retno Komala sari, Sonya Permata Saroh, Dewi Septiadi, Luhur SEPTIANA SEPTIANA Septiani, Risma Dyah Setya, Adhi Kumoro Setyazi, Gama Stefanus Khrismasagung Trikusumaadi, Stefanus Khrismasagung Susilowati Susilowati SUYONO Suyono Suyono Syafe’I, Imam Syafrudin Syafrudin Syahran Wael Umi Fathonah, Yuliana Umi Hijriyah Valent, Febriana Verawati, Liesta Wasehudin, Wasehudin Widyastuti, Dinda Yuli Arif Tribudi Yusianti Silviani Yusnida, Azma Zahra, Desti