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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences El-Hayah : Jurnal Biologi Biotropika Jurnal Al-Tadzkiyyah Journal of Tropical Biodiversity and Biotechnology Indonesian Journal of Chemistry Jurnal Analis Kesehatan Batoboh : Jurnal Pengabdian Pada Masyarakat Surya Abdimas Jurnal Biotek Cendekia Journal of Pharmacy JURNAL ANALIS LABORATORIUM MEDIK Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Ilmu dan Teknologi Kesehatan Indonesian Journal of Global Health research Lumbung Farmasi : Jurnal Ilmu Kefarmasian Sciscitatio : Journal for Biological Science Jurnal Farmasi Bioinformatics and Biomedical Research Journal Mestaka: Jurnal Pengabdian Kepada Masyarakat Journal of Biological Science and Education Indonesian Research Journal on Education Bengawan :Jurnal Pengabdian Masyarakat Journal of Research in Educational Management Rumphius Pattimura Biological Journal Media Kesehatan Politeknik Kesehatan Makassar Jurnal Pengabdian kepada Masyarakat Arumbai International Journal of Social and Education (INJOSEDU) Proceeding Of International Conference On Education, Society And Humanity Jurnal Pengabdian kepada Masyarakat Arumbai
Didik Wahyudi
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Journal : Biotropika

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Colchicine-Induced Genetic Variability in Porang (Amorphophallus muelleri): a Study Using SRAP Markers Wahyudi, Didik; Suyono; Resmisari, Ruri Siti; Mauludyah, Rohmatul
Biotropika: Journal of Tropical Biology Vol. 13 No. 2 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.02.04

Abstract

Mutation induction of porang both physically and chemically is widely used to increase the genetic variation of porang. However, mutation induction chemically by using colchicine is more effective than the other mutagens. Therefore, this study aims to assess the genetic diversity of porang after mutation induction using colchicine. Polyploidy induction is performed 30 days after planting of the porang plantlet. 0%, 0.01%, 0.02%, 0.03% and 0.04% colchicine was added to 12.5 ml of MS medium using a 0.22 µm Millipore filter. After 53 days, porang plantlets were ready for DNA isolation. A total of 10 SRAP primer combinations were used for DNA amplification. Genetic diversity in and between porang population was performed using POPGENE software. Clustering analysis using PAST Software was also performed to identify genetic variation. Mutation induction using colchicine significantly increased the genetic diversity of porang. Among the SRAP primer combinations, ME2-EM2 is the most effective in detecting the induced genetic variation in porang. Mutation induction using 0.03% colchicine is highly recommended as it produces significant genetic changes compared to normal porang. Porang mutant resulting from 0.03% colchicine treatment holds great potential for porang breeding programs, as it can generate novel phenotypic variations and increase the chances of obtaining superior genotypes with desirable agronomic traits.
Phenotypic Variation and RAPD Polymorphism of Pisang Kepok Local Cultivars (Musa acuminata x Musa balbisiana, ABB, Saba Subgroup) Wahyudi, Didik; Ilmi, Zahrobatul Lil; Hapsari, Lia
Biotropika: Journal of Tropical Biology Vol. 10 No. 3 (2022)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2022.010.03.03

Abstract

Pisang Kepok is a major local banana cultivar in Indonesia with high economic, social and cultural value. Particularly on the island of Java, there are several variations of Pisang Kepok recognised with their own local names, which makes difficulties in taxonomic identification and grouping. Morphological features are used in conventional banana cultivar classification, but they are deemed less precise due to their subjectivity, thus, it is supposed to be complemented with a molecular approach. This study aims to identify the phenotypic variation of Pisang Kepok local cultivars also their genetic polymorphism using Random Amplified Polymorphic DNA marker. Phenotypic variation was observed using 35 morphological characters. Six RAPD primers were used, i.e. OPA2, OPA3, OPA4, OPA11, OPA12, and OPA18. Clustering analysis, both phenotypic and genetic were performed using PAST v4.02. The morphological characterisation identified four variants of Pisang Kepok i.e. Kepok Abang, Kepok Putih, Kepok Manurun, and Kepok Australi; which all confirmed as ABB genome group. Phenotypic clustering showed that Pisang Kepok cultivars were separated into 3 clusters based on their local name, with a high similarity value of >90%. PCA biplot showed that the fruit flesh colour was the most important character contributed to the cultivar variation. RAPD marker also showed that each specimen was grouped according to its local name and source, with a similarity value of >80%. Both morphology and molecular (RAPD) markers resulted in the branching of Pisang Kepok which was closer to Pisang Klutuk than Pisang Barlin.
Co-Authors Afianti, Linda Dwi Afiati, Lely Agung, Rendyansah ahmad yani Aju Tjatur Nugroho Krisnaningsih Alfaruqi, Nuri Thobibatus Shofia Anak Agung Istri Sri Wiadnyani Andriani, Disa Anjarani, Ajeng Viona Putri Annisa, Nenden Nur Anwar, Muhamad Syaeful Ardani, Edvi Gracia Ardy Prian Nirwana, Ardy Prian Auliantika, Dinda Helsa Baehaqi Bayyinatul Muchtaromah Budhi, Dono Setya Chairul Amriyah Dian Puspita Sari Dwi Gusmalawati Endang Sutariningsih Soetarto Endang Widyastuti Ervin Medianti Fadhila, Zulfa Nur Fitriyah Fitriyah Gusmiati, Lutfiana Hasanah Hadiani, Dimas Pratidina Puriastuti Hasan, M. Nur Hidayatullah, Rafi Fuzan Ilmi, Zahrobatul Lil Indivia, Imey Tamara Isna Arofatun Nikmah, Isna Arofatun Istiqomah, Safitri Nur Juwanita, Agnes Kharisma, Viol Dhea Kusuma, Eka Wisnu Leondro, Henny Lia Hapsari M. Dimas Yudi Witjaksono Manshur, umar Mauludyah, Rohmatul Mimi Salmawati Mose, Windi Muhammad Saefi Mujahidin Ahmad Munandar, Hanozanor Fadli Nawang, Pungky Oke Anandika Lestari Pratimasari, Diah Prihandini, Peni Wahyu PUDJI ASTUTI Purwandani, Safitri Putri, Sheli Feliyan Rahmawati, Valentina Indah Rodiyati Azrianingsih Ruri Siti Resmisari Rusdi, Nurhidaya Saputra, Dedech Muhammad Sari, Retno Komala sari, Sonya Permata Saroh, Dewi Septiadi, Luhur SEPTIANA SEPTIANA Septiani, Risma Dyah Setya, Adhi Kumoro Setyazi, Gama Stefanus Khrismasagung Trikusumaadi, Stefanus Khrismasagung Susilowati Susilowati SUYONO Suyono Suyono Syafe’I, Imam Syafrudin Syafrudin Syahran Wael Umi Fathonah, Yuliana Valent, Febriana Verawati, Liesta Wasehudin, Wasehudin Widyastuti, Dinda Yuli Arif Tribudi Yusianti Silviani