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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences El-Hayah : Jurnal Biologi Biotropika Jurnal Al-Tadzkiyyah Journal of Tropical Biodiversity and Biotechnology Indonesian Journal of Chemistry Jurnal Analis Kesehatan Batoboh : Jurnal Pengabdian Pada Masyarakat Surya Abdimas Jurnal Biotek Cendekia Journal of Pharmacy JURNAL ANALIS LABORATORIUM MEDIK Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Ilmu dan Teknologi Kesehatan Indonesian Journal of Global Health research Lumbung Farmasi : Jurnal Ilmu Kefarmasian Sciscitatio : Journal for Biological Science Jurnal Farmasi Bioinformatics and Biomedical Research Journal Mestaka: Jurnal Pengabdian Kepada Masyarakat Journal of Biological Science and Education Indonesian Research Journal on Education Bengawan :Jurnal Pengabdian Masyarakat Journal of Research in Educational Management Rumphius Pattimura Biological Journal Media Kesehatan Politeknik Kesehatan Makassar Jurnal Pengabdian kepada Masyarakat Arumbai International Journal of Social and Education (INJOSEDU) Proceeding Of International Conference On Education, Society And Humanity Jurnal Pengabdian kepada Masyarakat Arumbai
Didik Wahyudi
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Genetic Diversity of Porang Populations (Amorphophallus Muelleri Blume) In Central Java and West Java Based on LEAFY Second Intron Marker Nikmah, Isna Arofatun; Azrianingsih, Rodiyati; Wahyudi, Didik
Journal of Tropical Life Science Vol 6, No 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.05

Abstract

Porang (Amorphophallus muelleri Blume) dispersed and grows well in Java island. This research aimed to determine the genetic diversity of porang populations in Central Java and West Java based on LEAFY second intron (nuclear genome encoding flower development). DNA samples of porang were from previous study, which are obtained from Central Java (Pamedaran, Grobogan, Wonogiri, Karangtengah) and West Java (Cisompet) as ingroup. A. variabilis from Pamedaran Brebes, A. ochroleucus, A. longituberosus, A. sumawongii, and A. tinekeae from genbank were treated as outgroup. LEAFY second intron was amplified using primers FLint2 F1 5’-CTTCCACCTCTACGACCAGTG-3’ and FLint2 R1 5’-TCTTGGGCTTGTTGATGTAGC-3’. Analysis using dnasp5 showed that 23 samples had 89 invariable sites and 139 variable sites that consisted of 12 singleton two variants sites, 118 parsimony-informative two variants sites and 9 Parsimony-informative three variants sites. The total of haplotype were 7 haplotypes where 16 ingroup samples clumped on haplotype 1 whereas 2 samples of Grobogan (Grobogan I and Grobogan III) clumped on haplotype 2 and outgroup samples occupied at their own haplotype. Genetic distance of all ingroup samples were 0-0,004 showing no differences among individuals. Analysis of phylogenetic tree using Maximum Parsimony, Neighbor Joining and Maximum Likelihood resulting a polytomy branch of ingroup samples except Grobogan which forms a separated sub clad. Key words: Genetic diversity, haplotype, Java, LEAFY, Porang.
Co-Authors Afianti, Linda Dwi Afiati, Lely Agung, Rendyansah ahmad yani Aju Tjatur Nugroho Krisnaningsih Alfaruqi, Nuri Thobibatus Shofia Anak Agung Istri Sri Wiadnyani Andriani, Disa Anjarani, Ajeng Viona Putri Annisa, Nenden Nur Anwar, Muhamad Syaeful Ardani, Edvi Gracia Ardy Prian Nirwana, Ardy Prian Auliantika, Dinda Helsa Bayyinatul Muchtaromah Budhi, Dono Setya Chairul Amriyah Dian Puspita Sari Dwi Gusmalawati Endang Sutariningsih Soetarto Endang Widyastuti Ervin Medianti Fadhila, Zulfa Nur Fitriyah Fitriyah Gusmiati, Lutfiana Hasanah Hadiani, Dimas Pratidina Puriastuti Hasan, M. Nur Hidayatullah, Rafi Fuzan Ilmi, Zahrobatul Lil Indivia, Imey Tamara Isna Arofatun Nikmah, Isna Arofatun Istiqomah, Safitri Nur Juwanita, Agnes Kharisma, Viol Dhea Kusuma, Eka Wisnu Leondro, Henny Lia Hapsari M. Dimas Yudi Witjaksono Manshur, umar Mauludyah, Rohmatul Mimi Salmawati Mose, Windi Muhammad Saefi Mujahidin Ahmad Munandar, Hanozanor Fadli Nawang, Pungky Oke Anandika Lestari Pratimasari, Diah Prihandini, Peni Wahyu PUDJI ASTUTI Purwandani, Safitri Putri, Sheli Feliyan Rahmawati, Valentina Indah Rodiyati Azrianingsih Ruri Siti Resmisari Rusdi, Nurhidaya Saputra, Dedech Muhammad Sari, Retno Komala sari, Sonya Permata Saroh, Dewi Septiadi, Luhur SEPTIANA SEPTIANA Septiani, Risma Dyah Setya, Adhi Kumoro Setyazi, Gama Stefanus Khrismasagung Trikusumaadi, Stefanus Khrismasagung Susilowati Susilowati SUYONO Suyono Suyono Syafe’I, Imam Syafrudin Syafrudin Syahran Wael Umi Fathonah, Yuliana Valent, Febriana Verawati, Liesta Wasehudin, Wasehudin Widyastuti, Dinda Yuli Arif Tribudi Yusianti Silviani