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EFFECT OF DATE FRUIT (PHOENIX DACTYLIFERA L.) EXTRACT ON TNFα LEVELS AND BRAIN WEIGHT OF ALZHEIMER’S MODEL RATS Marintan, Elita; Wasita, Brian; Magna, Adi
MNJ (Malang Neurology Journal) Vol. 8 No. 1 (2022): January
Publisher : PERDOSSI (Perhimpunan Dokter Spesialis Saraf Indonesia Cabang Malang) - Indonesian Neurological Association Branch of Malang cooperated with Neurology Residency Program, Faculty of Medicine Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.mnj.2022.008.01.6

Abstract

Background: Alzheimer's disease (AD) is a  neurodegenerative disorder marked by brain inflammation resulted in structural damage and brain dysfunction. Tumor necrosis factor α (TNFα) is a cytokine that plays an important role in inflammation. Dates fruit may help to fight oxidative stress and inflammation in the brain.Objective: To determine the effect of date fruit extracts on blood TNFα levels and brain weight of alzheimer’s model rats.Methods: This research is a laboratory experimental study by post-test only with control group design using alzheimer model rats. This study used 6 treatment groups with simple randomization. Each treatment group was represented by 8 Sprague Dawley rats. The normal control group (KN) was not induced by Hcy and was not given date palm extract, the negative control group (K-) was the Alzheimer's experimental rats which was not given the date palm extract, the positive group was the Alzheimer's experimental rats which was given the Donepezil (K+). Groups P1, P2, P3 were Alzheimer's experimental rats that were given date palm extract at a dose of 200, 400,800 mg / kgBW / day. The effect of date palm extract dosage on TNFα levels and brain weight were analyzed using the One Way Anova test followed by Tukey's post hoc test.Results: The difference in TNFα levels between groups showed a significant difference (p = 0.00). Meanwhile there was no significant difference in brain weight among all groups (p > 0,05). Conclusion: Date palm extract at doses of 200, 400, 800 mg / kgBW can decrease blood TNFα levels of Alzheimer’s model rats.
Evaluasi Efektifitas Malnutrition Screening Tool (Mst) Sebagai Alat Untuk Menentukan Risiko Malnutrisi Pada Pasien Geriatri ernawati, ammik; Wiboworini, Budiyanti; Wasita, Brian
Profesi (Profesional Islam) : Media Publikasi Penelitian Vol. 19 No. No.2 (2022): Jurnal PROFESI (Profesional Islam): Media Publikasi Penelitian
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) ITS PKU Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26576/profesi.v19iNo.2.94

Abstract

Kekurangan gizi masih merupakan masalah gizi utama baik saat pasien masuk dan selama pasien dirawat di rumah sakit.Pasien geriatri atau lanjut usia merupakan kelompok pasien dengan risiko malnutrisi yang tinggi. Oleh karena itu sangat penting untuk dilakukan identifikasi risiko malnutrisi sejak dini.Hal ini dapat dilakukan salah satunya dengan Malnutrition Screening Tool (MST) yangmerupakan alat skrining yang mudah, sederhana, dan cepat. Penelitian ini bertujuan untuk mengevaluasi efektifitas MST dalam menentukan risiko malnutrisi pada pasien geriatri. Observasi dilakukan pada 153 pasien geriatri di Instalasi Rawat Inap RSUD dr. Soediran Mangun Soemarso Kabupaten Wonogiri. Hasil menunjukkan bahwa MST memiliki sensitivitas yang tidak terlalu tinggi namun memiliki spesifisitas dan akurasi yang cukup baik dalam menentukan risiko malnutrisi berdasarkan kriteria IMT, asupan energi, dan persen lemak
Phenotype and Conditioning Medium on Umbilical Cord-Mesenchymal Stem Cell (UC-MSC) Kusnanto, Paulus; Purwanto, Bambang; Wasita, Brian; Widyaningsih, Vitri
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy Vol 24, No 2 (2023): VOLUME 24, NUMBER 2, August, 2023
Publisher : The Indonesian Society for Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24871/2422023127-131

Abstract

Stem cells are unspecialized cells that differentiate into more specialized cells for various tissues in the human body. Stem cells have the characteristics and ability to become themselves by performing various mitoses and differentiating different cells. Stem cells also have great potential for tissue regeneration. Detection and isolation of specific cells is very important in understanding biological processes such as development, aging, regeneration, and cell pathogenesis. Therefore, stem cells are present as an invaluable therapeutic option. Before stem cells can be applied, it is important to know the characteristics of the specific function cells or phenotypic markers. Isolation and characteristics of stem cells on culture morphology or immunotaining on specific markers. Immunohistochemistry can provide a general description of the expression of markers in a cell under certain conditions. Surface markers are the basis for identifying the characteristics and isolation of stem cells. The basic characteristics of stem cells are one that influences the outcome of the therapy to be carried out. The presence of marker cell surface expressions can also be a differentiator in searching for the origin of the stem cells used6. MSC culture method was performed with DMEM + 5% HPL. UC-MSCs with phenotype differentiation were found to be CD73+, CD90+, CD105+, Lin- while the parameters found were IL-6, SDF-1, BDNF, FGF, NGF, IL-12p70, IL-23, IL-18, IL-10 , VEGF, EGF, IL-17A, IFN-, IL-1β, PDGF, MCP-1.The results obtained are in accordance with the standard content of UC-MSC cells which can be continued as cell research.
The Relationship Between Nutritional Status and Cognitive Function in The Elderly In The UPT Area of The City Health Center Surakarta Wulansari, Indry; Wasita, Brian; Muthmainah
Nusantara Science and Technology Proceedings The 1st International Conference Muhammadiyah Yogyakarta – Hospital & Healthcare Management
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4203

Abstract

With age comes changes in the nervous system that are associated with a decline in cognitive function. Impaired cognitive function in the elderly is in the form of decreased memory, language, decision-making, and action. Nutritional status can be related to cognitive function. Measurement of nutritional status can be done by calculating body mass index (BMI) to determine the risk of malnutrition or obesity. Based on data from the Indonesian Psychogeriatric Organization (2019), states that the elderly aged 50-59 years experience a decrease in cognitive function in the form of forgetfulness with a mild category (Mild Cognitive Impairment) with a prevalence of 39%. To determine the relationship between nutritional status and cognitive function in the elderly in the UPT Puskesmas Surakarta City area. The design in this study was observational analytic with a Cross-Sectional approach. Samples in the study were taken by Simple Random Sampling of as many as 110 elderly people at the Surakarta City Health Center. Nutritional status in this study was measured by Body Mass Index (BMI) and cognitive function was carried out by interviewing the Mini-Mental State Examination (MMSE) questionnaire. Data analysis used the Chi Square test (?=0.05). In this study, there were elderly with a nutritional status less with a percentage of 50%, normal 6.4%, and more nutritional status 43.7% while elderly with moderate cognitive function decline with a percentage of 34.5%, mild cognitive function decline 49.1% and no decline in cognitive function with a percentage of 16.4%. The results of this study indicate that there is a significant relationship between nutritional status and cognitive function in the elderly in the UPT Puskesmas Surakarta City area with (p=0.001). There is a significant relationship between nutritional status and cognitive function in the elderly in the UPT Puskesmas Surakarta City area. Based on research (Dewi, 2019), states that the elderly with poor nutritional status is caused by changes in intestinal function, metabolic changes and deficiencies in nutrient intake while the elderly with more nutritional status experience changes in body composition with central fat accumulation in the abdomen so that it is related to decreased cognitive function.
Determination of Levels of Phytochemical Compounds (Saponins, Alkaloids, Tannins) In Ripe Fiber Extract of Palmyra Fruit (Borassus flabellifer Linn) Amarduan, Tince Bety; Sari, Yulia; Wasita, Brian
Nusantara Science and Technology Proceedings The 1st International Conference of Health Institut Kesehatan Mitra Bunda 2024
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4308

Abstract

Palmyra fruit (Borassus flabellifer L.) is one of the palm tree family that can be utilized as a source of of food. It also possess numerous benefits in medicinal applications, including secondary metabolites in the form of alkaloids, flavonoids, saponins, tannins, and terpenoids. All of which exhibit antioxidant, anti-inflammatory, and antimicrobial. Therefore, it is imperative to conduct quantitative phytochemical analysis to garner the composition of each compound in Palmyra fruit. This study aims to determine the level of saponins, alkaloids, and tannins in Palmyra fruit fiber extract originating from Werain village, Selaru, West Southeast Maluku. The extraction process was done using the maceration method with 96% ethanol solvent. Method: This study is geared toward quantitative laboratory-scale experiments to determine the sample level. Saponin and alkoloids were analyzed using gravimetry methods while tannins were analyzed using UV-Vis spectrophotometric. Results: it is revealed that saponins level is 1,02%; Alkaloids level is 1,5464; and tannins level is 1,0220%. Conclusion: The presence of saponins is 1,02%; Alkaloids is 1,5464; and tannins is 1,0220%.
Elevated Growol Flour Reduce Fasting Blood Glucose, HOMA-IR and Increase Insulin Level in Rat Model with Type 2 Diabetes Mellitus Nofia, Yasinta; Wasita, Brian; Susilawati, Tri Nugraha
Media Gizi Indonesia Vol. 17 No. 2 (2022): JURNAL MEDIA GIZI INDONESIA (NATIONAL NUTRITION JOURNAL)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mgi.v17i2.151-158

Abstract

The rise of glucose levels in the blood of patients with type-2 diabetes mellitus (T2DM) is attributed to the decrease of insulin secretion and the interruption of insulin activity. Growol is a fermented product made from cassava that has the potential to lower blood glucose levels in patients with T2DM. This study was aimed to evaluate the effects of oral administration of growol flour on the levels of blood glucose, insulin, and HOMA-IR. A total of 40 male Wistar rats were divided into 5 groups i.e., negative control group, positive control group taking metformin, and 3 treatment groups taking growol flour of 3.1, 6.2, and 9.3 (g/200gBW), respectively, for 2 weeks. Blood glucose levels were measured using Enzymatic Photometric Method GOD-PAP, insulin using the ELISA kit, and HOMA-IR using the formula. The Shapiro Wilk test was used to determine the normality of the data followed by the One-Way ANOVA test and Posthoc Tukey HSD test. The Paired T-test was used to see the difference of pre- and post-treatment levels of blood glucose, insulin, and HOMA-IR. There was a decrease (p<0.05) in blood glucose levels and HOMA-IR after the administration of low, moderate, and high doses of growol flour. In contrast, the levels of insulin increased (p<0.05) after the administration of low, moderate, and high doses. In conclusion, growol flour contributes to the maintenance of T2DM by lowering the levels of fasting blood glucose levels, as well as HOMA-IR, and increasing the levels of insulin in rats.
Pengaruh Fenol dan Saponin pada Madu Hutan Apis Dorsata dan molekul docking pada penanda preeklampsia yusari; Soetrisno; Prayitno, Adi; Wasita, Brian
Journal of Advanced Technology and Multidiscipline Vol. 3 No. 2 (2024): Journal of Advanced Technology and Multidiscipline
Publisher : Faculty of Advanced Technology and Multidiscipline Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jatm.v3i2.60655

Abstract

Molecular docking is an important computational method for drug design. It can be used to predict receptor binding interactions with ligans. In addition, phenol-derived compounds and Saponins are also reported to have various activities such as anti-hypertensive, anti-inflammatory and anti-apoptosis agents. The purpose of this study is to predict whether the Phenol and Saponin compounds are active as anti-inflammatory agents and also to ensure that the binding interactions are stable before and after the docking calculations. The protocol is carried out in accordance with the standards set by the LPPT-UGM Testing Laboratory. The tools, programs, and applications used in this study were Lenovo IdeaPad Flex 5 Processor, AMD Ryzen 5000 Series 5, AutoDock Tools (v1.5.6), Biovia Discovery Studio, AutodockVina, Swiss ADME, VegaZZ, Pubchem, and the and the pkCSM web server (http://biosig.unimelb.edu.au/pkcsm/). Trans-ADFH three-dimensional structure, as a test compound, and Prednisolone, as a standard compound, were downloaded from https://pubchem.ncbi.nlm.nih.gov/. The phytochemical analysis of ADFH includes phenol and saponin. Structure of PlGF 3D (IRV6) and VEGF (4KZN). Identification of ligands and proteins prepared using Pubchem results of molecular docking between preeclampsia through examination of PlGF and VEGF with phenols and saponins Identification of protein ligands using Pubchem, i.e., PlGF (IRV6) with phenol produced 2 interactions and 2 amino acid residues, while saponins produced 2 interactions and 6 residues. pharmacokinetics and toxicity using Swiss ADME, i.e., phenol and saponins are non-mutagenic to bacteria; the maximum safe dose for humans is 0.54 log mg/kg/day; it does not cause toxicity to the heart; the estimated dose for animals is 2.471 mol/kg; it does not cause allergies; it does not cause liver damage; a dose of 0.288 logμ/L can inhibit the growth of 50% of the protozoa T. Pyformis. The content of phenols and saponins in Apis Dorsata forest honey has a high docking score from the original ligand. Molecular docking on phenols and saponins identified PlGF with scores of -2.85, -2.85, and -2.85 kcal/mol, while saponins have +2.84, +2.29, and +2.29 kcal/mol values, which are stated to be better results than the original ligands. This means that phenol has a role as a standard drug that can have an effect on lowering PlGF levels for people with preeclampsia. Molecular docking on VEGF identified phenol with scores of -2.88, -2.88, and -2.88 kcal/mol, and saponins have +1.36, +3.46, and +1.36 kcal/mol values, which are stated to have better results than the original ligands. This means that saponins have a role as a standard drug that can have an effect on increasing VEGF levels for people with preeclampsia.
The Effect of Combined Extracts of Sappan Wood (Caesalpinia sappan L.) and Gotu Kola (Centella asiatica L.) in Improving Diabetic Condition in Rats Binti Maulina; Wasita, Brian; Febrinasari, Ratih Puspita
Jurnal Gizi dan Pangan Vol. 17 No. 1 (2022)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.931 KB) | DOI: 10.25182/jgp.2022.17.1.37-46

Abstract

This study aimed to determine the efficacy of combination of sappan (secang) wood and gotu kola extracts in reducing insulin resistance and Malondialdehyde (MDA) levels in diabetic rats induced by Streptozotocin (STZ) 65 mg/kg Body Weight (BW) and Nicotinamide (NA) 230 mg/kg BW. Forty-two male Sprague Dawley rats weighing ±200 g were divided into 7 groups: 1) control, 2) glibenclamide 0.45 mg/kg BW, 3) sappan wood extract (CS) 250 mg/kg BW, 4) gotu kola extract (CA) 500 mg/kg BW, 5) 1st combination of extracts of sappan wood and gotu kola (CSCA1) 125 mg/kg BW + 750 mg/kg BW, 6) 2nd combination (CSCA2) with 250 mg/kg BW + 500 mg/kg BW, and 7) 3rd combination (CSCA3) with 375 mg/kg BW + 250 mg/kg BW. The insulin resistance levels were measured using the HOMA-IR index based on fasting blood glucose and insulin. The Thiobarbituric Acid Reactive Substance (TBARs) method was used to measure MDA levels. All measurements were taken before treatment, 14 days after treatment, and 21 days after treatment. The group receiving CSCA3 showed significant reduction in insulin resistance (-3.32±0.05) and MDA levels (-2.04±0.37 nmol/ml) on Day 21 after treatment. The CSCA3 treatment did not show statistically different result compared to glibenclamide treatment (p>0.05). Hence, CSCA3 treatment was considered as the best proportion of sappan wood and gotu kola extracts mixture and the result is comparable to glibenclamide. This study shows that the combination of sappan wood and gotu kola extracts has the potential to be developed as a functional drink for people with diabetes.
Effects of Moringa oleifera extract on inflammaging markers, muscle mass, and physical endurance in geriatric mice model Budiningsih, Fatichati; Purwanto, Bambang; Wasita, Brian; Poncorini, Eti
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.2052

Abstract

A comprehensive approach to managing frailty is required due to the increasing number of elderly. Physical frailty, associated with inflammatory processes and a loss of muscle mass, can significantly impair health status. The aim of this study was to determine the effect of Moringa oleifera Lam. leaf extract on frailty in geriatric mice model. An experimental study was conducted using a post-test-only control group design using  Mus musculus Balb/C mice aged eight weeks. The animals were divided into five groups: negative control group received intraperitoneal injections of normal saline (0.5 cc/day) for eight weeks, positive control group received D-galactose (150 mg/kg body weight (BW)/day) only, and three treatment groups treated with 150 mg/kg of D-galactose intraperitoneally for eight weeks followed by 100 mg/kg BW, 200 mg/kg BW, and 400 mg/kg BW of M. oleifera leaf extract orally for five weeks. At the end of the study, the blood levels of malondialdehyde (MDA), transforming growth factor-beta (TGF-β) and caspase-3, as well as caspase-3 expression in skeletal muscle, skeletal muscle mass fibrosis, and physical endurance were measured. The results showed that a dose of 400 mg/kg/day of M. oleifera leaf extract had the most significant effect on lowering MDA, TGF-β, and caspase-3 expression in skeletal muscle tissue, skeletal muscle fibrosis, and improved physical endurance compared to other groups (p<0.001). This study highlights that M. oleifera leaf extract reduced frailty in geriatric mice model-induced using D-galactose by reducing inflammaging factors, thereby improving physical endurance.
Differences in Age Estimated by the Kvaal Method on Right and Left Mandibular Canines Kusumaningrum, Sulistyani; Wasita, Brian; Wujoso, Hari; Rahayu, Rachmi Fauziah; Hermansyah, Muhamad Lukman; Lawei, Tharina
Indonesian Journal of Medicine Vol. 6 No. 2 (2021)
Publisher : Masters Program in Public Health, Sebelas Maret University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (31.495 KB) | DOI: 10.26911/theijmed.2021.6.2.401

Abstract

Background: The radiographic method for deter­mining the estimated age has the advantage of being non-invasive and the orthopantomogram images are digitally processed. Canines have a strong corre­la­tion with chronological age and have good resistance and large pulp space. This study aimed to determine the difference in age estimates using the Kvaal method on the right and left mandibular canines.Subjects and Method: There were 80 ortho­pantomogram samples from dental and oral clinic patients who had undergone orthopantomography at the Radiology Installation of Dr. Moewardi Hos­pital, from January 2019 to December 2020. The estimated age of right and left canines was calcu­lated based on the Kvaal method and performed a T-test.Results: At the estimated age of the right and left mandibular canines determined by the Kvaal formula, the T-test was performed showing a mean of 38.3 years for the right mandibular canine (Mean= 38.3; SD= 6.7) and 38.2 year the left mandi­bular canine (Mean= 38.2; SD= 8.9), with p = 0.910. Data analysis showed that there was no difference in age estimation using the Kvaal method on the right and left mandibular canines.Conclusion: There was no difference in age estimates by the Kvaal method on the right and left mandibular canines.Keywords: Oratopantomogram, Metoday Qual, Kaninus MandibulaCorrespondence: Sulistyani Kusumaningrum. Department of Radiology Dr. Moewardi Hospital / Faculty of Medicine Universitas Sebelas Maret, Surakarta. Email: kusumasulis1@gmail.com.Indonesian Journal of Medicine (2021), 06(02): 206-211https://doi.org/10.26911/theijmed.2021.06.02.09
Co-Authors Adam Fauzi Adi Magna, Adi Adi Prayitno Afdhaliya, Noor Khalwati Agus Kristiyanto Amarduan, Tince Bety Ambar Mudigdo Amilia Yuni Damayanti Ana Rima Anugrah, Tessa Septian Ari Probandari Asrial, An A. Bambang Purwanto Binti Maulina Brahmana, Ivanna Beru Budiningsih, Fatichati Budiyanti Wiboworini Cindikya Saftiari Dewi Cr. Siti Utari Cr. SitiUtari Darmayani, Aritantri David Perdana Dentiko Wasis Aulia Dewi Okta Anggraini Didik Prasetyo Diffah Hanim Dono Indarto Eka Puspita Sari Eka Tri Wulandari, Eka Tri Ellistasari, Endra Yustin ernawati, ammik Eti Poncorini Pamungkasari Fadila, F Gardjito, Fajar Baskoro Gunawan, Timotheus A. Hari Wujoso Harsono Salimo Hermansyah, Muhamad Lukman Herviana, Herviana Ida Nurwati Idulhaq, Mujaddid Irawan, Amelia Tjandra Irma Suryani Jati, Agus Kariosentono, ⁠⁠Harijono Kusumaningrum, Sulistyani Laqif, Abdurrahman LAWEI, THARINA Marintan, Elita Muchamad Syamsulhadi, Muchamad Muhammad Riza Mulianto, ⁠Nurrachmat MULYOTO PANGESTU Muthaminah, Muthaminah Muthmainah Muthmainah Muthmainah Nanang Wiyono Nofia, Yasinta Novan Adi Setyawan Nur Shani Meida Nursulistyo, Fajar Paramasari Dirgahayu Paulus Kusnanto Pesik, Riza N. Poncorini, Eti Primisawitri, Pratiwi Prasetya Pudjiastuti, Anggit puguh santoso, puguh Rachmi Fauziah Rahayu Rahayu, Rachmi F. Rahmah Rahmah Ratih Dewi Yudhani Ratih Puspita Febrinasari Reviono Reviono Risya Cilmiaty, Risya Rita Cempaka Riza Deviana Riza Novierta Pesik Rizma, Amalina Sabariyah, Lely Sahara, Nita Santi, Nur Saputra, Rhyan Darma Senyum Indrakila Setianto, Budi Y. Sholikah, Tri Agusti Siswanto, Febby Gunawan Siti Maesaroh Soestrisno, Soestrisno Soetrisno Soetrisno Soetrisno Sri Lestariningsih Suyatmi Suyatmi Tamaulina Br Sembiring Taufiqurrahman, Surya Tedjo Danudjo Tonang Dwi Ardyanto Tri Harsono Tri Nugraha Susilawati Triyanta Yuli Pramana Uki Retno Budihastuti Umi Mahmudah Vitri Widyaningsih Wardhani, Lusi O. Wardhani, Lusi Oka Wayanshakty, Janur Widhiati, Suci Wulandari, Eka T. Wulansari, Indry Yarsa, Kristanto Y. Yarso, Kristanto Yuli Yudhani, Ratih D. Yudhistira, Muhammad Yurizar Yundari, Yundari yusari Yusup Subagio Yusup Subagio Sutanto Yuyun Yueniwati Zahidah, Fairuz Zahrah, Fathimah Afifah Zaidah, Lailatuz