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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Teknologi Perikanan dan Kelautan Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Akuakultur Rawa Indonesia ILMU KELAUTAN: Indonesian Journal of Marine Sciences Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Omni-Akuatika Proceeding Simposium Nasional Kelautan dan Perikanan Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Media Akuatika: Jurnal Ilmiah Jurusan Budidaya Perairan Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Makara Journal of Science Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine
MUNTI YUHANA
Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan - IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Library & Information Science Medicine & Pharmacology Social Sciences Veterinary

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Effectivity of prebiotic mannan oligosaccharides as the immunity enhancer and growth response on whiteleg shrimp Litopenaeus vannamei against white spot disease Prastiti, Linuwih Aluh; Yuhana, Munti; Widanarni, Widanarni,
Jurnal Akuakultur Indonesia Vol. 17 No. 1 (2018): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2140.11 KB) | DOI: 10.19027/jai.17.1.81-86

Abstract

ABSTRACT This study aimed to evaluate the immune response and growth performance of white shrimp administered with prebiotic mannan oligosaccharides (MOS) with dosages of (0%, 0.2%, 0.4%, and 0.8% in diet) and used in the feeding trial. Shrimps (Litopenaeus vannamei) (the initial average weight was 3.416±0.064 g) were fed at satiation, three times a day. A completely randomized design was used  in the study. Shrimps were cultured at the stock density of 15 shrimps 40/L for each treatment in triplicates. After 30 days of the feeding trial, shrimp were challenged with white spot syndrome virus filtrate by intramuscular injection. The total gut bacteria, total haemocyte count (THC), phenoloxydase (PO), and respiratory burst (RB) activity were observed 4 times, before the experiment, day 30th before challenge test, day 32nd after challenge test, and day 36th the end of the experiment. The shrimp survival was observed at day 36th to evaluate the immune responses. The results showed that THC, PO activity, RB activity, growth performance, and shrimp survival administered with prebiotic 0.8% were significantly higher (P<0.05) than control. The administration of prebiotic with dose 0.8% was the best result and could effectively improve the immune responses and growth performance of whiteleg shrimp. Keywords: prebiotic, whiteleg shrimp, white spot disease  ABSTRAK Tujuan dari penelitian ini adalah untuk mengevaluasi respons imun dan performa pertumbuhan pada udang vaname yang diberi prebiotik mannan-oligosaccharides (MOS) dengan dosis berbeda (0%, 0,2%, 0,4%, dan 0,8%) pada pakan. Udang vaname (Litopenaeus vannamei) (dengan rata-rata bobot 3,41 ± 0,06 g) diberi pakan tiga kali sehari secara at satiation. Penelitian ini menggunakan rancangan acak lengkap. Udang dipelihara dengan kepadatan 15 ekor per 40/L pada setiap perlakuan dengan tiga kali pengulangan. Setelah 30 hari pemberian pakan, udang diuji tantang menggunakan white spot syndrome virus dengan diinjeksi secara intramuskular. Total bakteri usus, total haemocyte count (THC), aktivitas phenoloxydase (PO), dan aktivitas respiratory burst (RB) diamati 4 kali, yaitu sebelum perlakuan hari ke-30 sebelum uji tantang, hari ke-32 setelah uji tantang, dan hari ke-36 pada akhir penelitian. Hasil penelitian menunjukkan bahwa THC, aktivitas RB, aktivitas PO, performa pertumbuhan, dan kelangsungan hidup yang diberi prebiotik dengan dosis 0,8% lebih tinggi (P<0,05) jika dibandingkan dengan kontrol (dosis 0%). Pemberian prebiotik dengan dosis 0,8% merupakan hasil terbaik dan secara efektif mampu meningkatkan respons imun dan performa pertumbuhan pada udang vaname. Kata kunci: prebiotik, udang vaname, white spot disease  
Supplementation of Nodulisporium sp. KT29 induced by Vibrio harveyi as an immunostimulant for controlling vibriosis in vannamei white shrimp under marine culture system Wahjuningrum, Dinamella; Efianda, Teuku Reza; Tarman, Kustiariyah; Yuhana, Munti; Effendi, Irzal; Saputra, Fazril
Jurnal Akuakultur Indonesia Vol. 19 No. 2 (2020): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.19.2.95-105

Abstract

ABSTRACT The purpose of this study is to evaluate the effectiveness of Nodulisporium sp. KT29 supplementation with variousVibrio harveyi induction in feed against vibriosis in vannamei white shrimp. The study design included KP (positivecontrol without supplementation Nodulisporium sp. KT29 and infected with V. harveyi), KN (negative controlswithout Nodulisporium sp. KT29 and infected with physiological solution), NT (treatment of supplementationNodulisporium sp KT29 20 mL/kg and infected with V. harveyi), NM (treatment of supplementation testNodulisporium sp. KT29 induction of V. harveyi dead cell 20 mL/kg and infected V. harveyi), NH (treatment ofsupplementation Nodulisporium sp. KT29 induction of V. harveyi live cell 20 mL/kg and infected with V. harveyi).The study parameters included inhibition zone, resistance, immune responses, and hemolim glucose. The resultsshowed Nodulisporium sp. KT29 with induction treatment raised antibacterial activity with best treatment of NMand NH (P<0.05). The results of V. harveyi infection resistance presented NM treatment of 20 mL/kg increasesurvival in vannamei shrimp reached 72.2% (P<0.05). In addition, the same treatment increase the immuneresponse activity and decrease the activity of hemolim glucose. It could be concluded that providing NM 20 mLtreatment boosted the resistance and the immune system in vaname shrimp to control vibriosis reared at the sea.Keywords: antibacterial, β-glucan, induced, Nodulisporium sp. KT29, Vibrio harveyi ABSTRAK Tujuan penelitian yaitu mengevaluasi efektivitas suplementasi Nodulisporium sp. KT29 dengan berbagai perlakuaninduksi Vibrio harveyi dalam pakan terhadap pengendalian vibriosis pada udang vaname yang dibudidayakan dilaut. Rancangan penelitian meliputi KP (kontrol positif tanpa suplementasi Nodulisporium sp. KT29 dan diinfeksiV. harveyi), KN (kontrol negatif tanpa Nodulisporium sp. KT29 dan diinfeksi larutan fisiologis), NT (perlakuanuji suplementasi Nodulisporium sp. KT29 20 mL/kg dan diinfeksi V. harveyi), NM (perlakuan uji suplementasiNodulisporium sp. KT29 diinduksi sel mati V. harveyi 20 mL/kg dan diinfeksi V. harveyi), NH (perlakuan ujisuplementasi Nodulisporium sp. KT29 induksi sel hidup V. harveyi 20 mL/kg dan diinfeksi V. harveyi). Parameterpenelitian meliputi zona hambat, resistensi, respons imun, dan glukosa hemolim. Hasil penelitian menunjukkanNodulisporium sp. KT29 dengan perlakuan induksi dapat meningkatkan aktivitas antibakteri dengan perlakuanterbaik NM dan NH (P<0.05). Hasil pengamatan resistensi infeksi V. harveyi menunjukkan perlakuan NM 20 mL/kg dapat meningkatkan kelangsungan hidup pada udang vaname mencapai 72.2% (P<0.05). Perlakuan yang samajuga meningkatkan respons imun dan menurunkan aktivitas glukosa hemolim. Disimpulkan bahwa pemberianNM 20 mL dapat meningkatkan resistensi dan sistem imun udang vaname terhadap pengendalian vibriosis di laut.Kata kunci : antibakteri, β-glucan, induksi, Nodulisporium sp. KT29, Vibrio harveyi,
Growth performance, immune response, and resistance of Nile tilapia fed paraprobiotic Bacillus sp. NP5 against Streptococcus agalactiae infection Aldy Mulyadin; Widanarni, Widanarni; Yuhana, Munti; Wahjuningrum, Dinamella
Jurnal Akuakultur Indonesia Vol. 20 No. 1 (2021): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.20.1.34-46

Abstract

ABSTRACT This study was aimed to evaluate the effectiveness of Bacillus sp. NP5 paraprobiotic administration through commercial feed on growth performance, immune response, and resistance of Nile tilapia against Streptococcus agalactiae infection. Bacillus sp. NP5 paraprobiotic was produced through heat-inactivation at 95°C for 1 h, then performed a viability test on tryptic soy agar (TSA) media and incubated for 24 hours. Paraprobiotics could be used whether the bacteria did not grow on the TSA media. This study used a completely randomized design, containing three treatments with five replications, i.e. 1% (v/w) probiotic addition, 1% (v/w) paraprobiotic addition, and no addition of probiotic or paraprobiotic (control). The experimental fish were reared for 30 days. On day 31 of rearing, fish were challenged with S. agalactiae (107 CFU/mL) through intraperitoneal injection route, while the negative control was injected with PBS. This study results significantly improved growth performances and immune responses (P<0.05), compared to control after 30 days of probiotic and paraprobiotic Bacillus sp. NP5 administration. After challenge test, increased immune responses in probiotic and paraprobiotic of Bacillus sp. NP5 treatment had higher survival rates (P<0.05) than positive control. The administration of Bacillus sp. NP5 probiotic and paraprobiotic through commercial feed were effective in increasing growth performance, immune response, and resistance of Nile tilapia against S. agalactiae infection. Keywords: Bacillus sp. NP5, Nile tilapia, paraprobiotic, Streptococcus agalactiae ABSTRAK Penelitian ini bertujuan mengevaluasi efektivitas pemberian paraprobiotik Bacillus sp. NP5 melalui pakan dalam meningkatkan kinerja pertumbuhan, respons imun, dan resistansi ikan nila terhadap infeksi Streptococcus agalactiae. Proses pembuatan bakteri paraprobiotik yaitu Bacillus sp. NP5 diinaktivasi panas pada suhu 95°C selama 1 jam, dilanjutkan dengan pengujian viabilitas dengan menyebarkannya pada media tryptic soy agar kemudian diinkubasi selama 24 jam. Jika bakteri tidak tumbuh, maka paraprobiotik dapat digunakan. Penelitian ini menggunakan rancangan acak lengkap dengan tiga perlakuan dan lima ulangan, yaitu penambahan probiotik 1% (v/w), penambahan paraprobiotik 1% (v/w), dan tanpa penambahan probiotik atau paraprobiotik (kontrol). Ikan perlakuan dipelihara selama 30 hari dan pada hari ke-31, ikan diuji tantang dengan S. agalactiae (107 CFU/mL) melalui injeksi intraperitoneal, sementara perlakuan kontrol negatif diinjeksi dengan PBS. Hasil penelitian setelah 30 hari pemberian probiotik dan paraprobiotik Bacillus sp. NP5 menunjukkan kinerja pertumbuhan dan respons imun yang meningkat signifikan (P<0.05) dibandingkan dengan kontrol. Pascauji tantang, peningkatan respons imun pada perlakuan probiotik dan paraprobiotik Bacillus sp. NP5 menunjukkan tingkat kelangsungan hidup yang lebih tinggi (P<0.05) dibandingkan kontrol positif. Pemberian probiotik dan paraprobiotik Bacillus sp. NP5 melalui pakan dapat meningkatkan kinerja pertumbuhan, respons imun, dan resistansi ikan nila terhadap infeksi Streptococcus agalactiae. Kata kunci: Bacillus sp. NP5, ikan nila, paraprobiotik, Streptococcus agalactiae
Synbiotic microcapsule dietary supplementation for prevention against co-infection diseases in Pacific white shrimp: a limited field experiment Munti Yuhana; Tambun, Andreas; Widanarni, Widanarni; Afiff, Usamah
Jurnal Akuakultur Indonesia Vol. 21 No. 2 (2022): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.21.2.125-132

Abstract

This study aimed to evaluate the effectivity of microencapsulated synbiotic (MS), Bacillus sp. NP5 and mannan oligosaccharide (MOS) dietary in different feeding frequencies in Pacific white shrimp culture field experiment. The MS was administered as a feed supplementation to enhance the immunity for prevention against co-infection with WSSV (White Spot Syndrome Virus) and Vibrio harveyi. The synbiotic was microencapsulated by the spray dryer method. Shrimps were reared in the floating net cages in the pond. Treatments included the administration of MS at different frequencies i.e, daily (A), twice a week (B), once a week (C), and without MS supplementation (consisted of negative and positive controls) with a feeding rate of 6% of shrimp biomass (5 times a day). During the challenge trial, shrimps were removed and further reared in plastic tanks, for 7 days. The shrimps (except negative control treatment) were intramuscularly injected by WSSV filtrate at the infective dosage of 10-4 copies.ml-1. Twenty four hours after WSSV injection the shrimps were immersed in the water-containing cells suspension of V. harveyi at the cell’s population dosage of 106 CFU.ml-1. Immune responses were observed for 7 days after experimental infection. The shrimps that have been treated with daily MS supplementation (A) showed better immune responses i.e., total haemocyte counts, phenoloxidase, respiratory burst, and the lower pathogenic cells abundance in the intestine compared to other treatments groups.
The effects of different dosage applications of Bacillus sp. NP5 para-probiotic on the growth performance and resistance of Nile tilapia against Streptococcus agalactiae infection Widanarni; Sukenda; Annisa Astri Anggraeni; Rr. Bellya Anasti Maharani; Aldy Mulyadin; Munti Yuhana
Jurnal Akuakultur Indonesia Vol. 21 No. 2 (2022): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.21.2.186-197

Abstract

The aim of the study is to test the effectiveness of adding various paraprobiotic doses of Bacillus sp. NP5 through feed in improving the immune responses and resistance of tilapia juvenile (Oreochromis niloticus) to infection of Streptococcus agalactiae. Bacillus sp. NP5 paraprobiotic was produced through heat-inactivation at 95°C for 1 h. This study used a completely randomized design, which consisted of four treatments with three repetitions. The test feed was enriched with 1% paraprobiotic with a density of 108, 109, and 1010 CFU ml-1. The tilapia juvenile (10.29 ± 0.22 g) were reared for 30 days and on day 31, the fish was challenge tested with S. agalactiae (107 CFU ml-1) injected by intraperitoneal route unless the negative control was injected with PBS. The results of this study showed significantly improved growth performances and immune responses (P<0.05), compared to the control group after 30 days of paraprobiotic Bacillus sp. NP5 administered. The increased of immune responses in paraprobiotic of Bacillus sp. NP5 showed a higher survival rate (P<0.05), than positive control after the challenge tested with S. agalactiae. Administration of paraprobiotic Bacillus sp. NP5 through the commercial feed able to improve the growth performances, immune responses, and resistance of nile tilapia against S. agalactiae infection with the best result at dose of 1010 CFU ml­-1.
Characterization and molecular detection of pathogenicity and antibiotic resistance genes in Vibrio parahaemolyticus isolated from Pacific white shrimp Muhammad Arif Mulya; Fachriyan Hasmi Pasaribu; Usamah Afiff; Munti Yuhana
Jurnal Akuakultur Indonesia Vol. 21 No. 1 (2022): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.21.1.81-92

Abstract

ABSTRACT This study aimed to conduct the characterization and molecular detection of the pathogenicity and antibiotic-resistance genes in Vibrio parahaemolyticus, as the causative agent of vibriosis in Pacific white shrimp. The V. parahaemolyticus isolates were collected from the shrimp’s hepatopancreas, before biochemical test and polymerase chain reaction (PCR) sequencing of the 16S rRNA gene confirmation. The hemolysis test and PCR were applied to detect the presence of virulence genes, namely toxR, thermostable direct haemolysin (tdh), and tdh-related haemolysin (trh). The Kirby-Bauer method was used for characterizing the resistance patterns against ampicillin (AMP), tetracycline (TET), cyprofloxacin (CIP), enrofloxacin (ENR), and chloramphenicol (CHL). The biochemical tests and PCR-16SrRNA gene sequencing confirmed that 12 isolates belonged to V. parahaemolyticus that were further verified by amplification of the toxR gene in 382 bp (100% of the isolates). The alpha hemolysis activity was also confirmed by the amplicon of 199 bp in all isolates. All V. parahaemolyticus isolates showed their resistance to AMP and 42% of the isolates were TET-resistant. However, no resistance was shown to CIP, ENR, and CHL. The PCR-based analysis resulted a detectable resistance gene of ampC (42% of the isolates) and tetB (83% of the isolates). Keywords: antibiotics, shrimp, resistance, virulency, Vibrio parahaemolyticus ABSTRAK Penelitian ini bertujuan untuk melakukan karakterisasi dan deteksi molekular dari gen patogenisitas dan resistansi antibiotik pada Vibrio parahaemolyticus, agen penyebab vibriosis pada udang vaname. Isolat V. parahaemolyticus dikoleksi dari hepatopankreas, diuji secara biokimiawi dan selanjutnya dikonfirmasi dengan polymerase chain reaction (PCR)-sekuensing dari gen 16S rRNA. Tes hemolisis dan metode PCR diterapkan untuk mendeteksi keberadaan gen virulensi toxR, thermostable direct haemolysin (tdh) and tdh-related haemolysin (trh). Metode Kirby Bauer digunakan untuk karakterisasi pola resistansi terhadap ampisilin (AMP), tetrasiklin (TET), kloramfenikol (CHL), siprofloksasin (CIP) dan enrofloksasin (ENR). Uji biokimia dan sekuensing gen PCR-16SrRNA memastikan bahwa 12 isolat adalah V. parahaemolyticus yang selanjutnya diverifikasi dengan amplifikasi gen toxR berukuran 382 bp (100% isolat). Aktivitas alfa hemolisis juga dikonfirmasi dengan amplikon PCR (199 bp) di semua isolat. Seluruh isolat V. parahaemolyticus menunjukkan resistansinya terhadap AMP, 42% resistan TET, tidak ada resistansi yang ditunjukkan pada CIP, ENR dan CHL. Analisis berbasis PCR menghasilkan gen resistan yang terdeteksi dari gen ampC (42% isolat) dan gen tetB (83% isolat). Kata kunci: Antibiotik, udang, resistansi, virulensi, Vibrio parahaemolyticus
Mini-review: Utilization of Vibrio parahaemolyticus virulence coding genes for early detection of acute hepatopancreatic necrosis disease (AHPND) Yuhana, Munti; Afiff, Usamah
Jurnal Akuakultur Indonesia Vol. 22 No. 1 (2023): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.22.1.87-96

Abstract

The ability to track the presence of pathogenic Vibrio parahaemolyticus, an AHPND causative agent in shrimp is one of the keys to controlling this infectious disease. A reputable disease diagnosis is appreciated as the ability to track the pathogenic infection when the host abnormality is undetectable due to the low pathogenic cell concentration. This mini-review article discusses the selected virulence encoding genes as molecular markers and the steps of standard validation methods in the application for early detection of AHPND disease. The proper diagnosis method is crucial to prevent the spread of Vibriosis AHPND which significantly results in economic losses for shrimp farmers. In this early warning system, we need a molecular method available for quick detection by applying the tracking tools that can discriminate pathogenic V. parahaemolyticus AHPND strain. Several types of potential genes that can be developed into tracking devices for infectious Vibriosis are pathogenic genes encoding the virulence factor. Through several stages of testing the selected virulence encoding genes will be developed into molecular markers. The polymerase chain reaction method and several of its variants have been widely applied using selected molecular markers. Furthermore, the use of molecular markers for the diagnosis of AHPND disease in shrimp must be validated by determining aspects of sensitivity, detection specificity, repeatability consistency, and reproducibility Keywords: virulence-encoding gene, Vibrio parahaemolyticus, AHPND, diagnose, molecular marker ABSTRAK Kemampuan melacak keberadaan patogen Vibrio parahaemolyticus, agen penyebab AHPND pada udang adalah salah satu kunci untuk mengendalikan penyakit menular ini. Diagnosis penyakit yang memiliki reputasi baik akan dihargai karena mampu untuk melacak infeksi patogen ketika tanda-tanda abnormalitas pada inang belum terdeteksi karena konsentrasi sel patogen yang masih rendah. Makalah mini-review ini membahas tentang tahap-tahap metode validasi standardalam aplikasi gen penyandi virulensi terseleksi untuk deteksi dini penyakit AHPND. Metode diagnosis yang tepat sangat penting untuk mencegah penyebaran Vibriosis AHPND yang secara signifikan mengakibatkan kerugian ekonomi bagi petambak udang. Dalam sistem peringatan dini ini, diperlukan metode molekular yang tersedia untuk deteksi cepat dengan menerapkan alat pelacak yang mampu membedakan patogen V. parahaemolyticus strain AHPND. Beberapa jenis gen potensial yang dapat dikembangkan menjadi alat pelacak penyakit Vibriosis menular adalah gen patogen yang mengkodekan faktor virulensi. Beberapa tahapan pengujian harus dilakukan untuk menjadikan gen penyandi virulensi terpilih sebagai kandidat yang akan dikembangkan menjadi penanda molekular. Metode polymerase chain reaction dan beberapa variannya telah banyak diterapkan dengan menggunakan penanda molekular terseleksi. Selanjutnya pemanfaatan penanda molekular untuk diagnosis penyakit AHPND pada udang harus dilakukan validasi dengan menentukan aspek sensitivitas, spesifisitas deteksi, konsistensi pengulangan, dan reprodusibilitas Kata Kunci: gen penyandi virulensi, Vibrio parahaemolyticus, AHPND, diagnosis, penanda molekuler
The antibacterial activity of clove Syzygium aromaticum extract and its effects on the survival rate of hybrid grouper Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂ infected with Vibrio alginolyticus Ode, Inem; Sukenda; Widanarni; Dinamella Wahjuningrum; Munti Yuhana; Mia Setiawati
Jurnal Akuakultur Indonesia Vol. 22 No. 1 (2023): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.22.1.1-11

Abstract

Vibrio alginolyticus which becomes the main cause of vibriosis disease in grouper culture and causes great economic loss in Asian aquaculture industries. This study investigated the antibacterial activity of clove extract and the effect of adding clove powder to the diet on survival cantang grouper infected with V. alginolyticus. The clove extraction used a maceration method. Two dose levels of clove powder were used, namely 10 and 15 g kg−1. The control treatments without clove powder supplementation contained positive control (CP) and negative control treatment (CN). The results found that the clove extract contained five major compounds. The highest compound was phenol, 2-methoxy-4-(2-propenyl)-Eugenol (64.07%). Phytochemical analysis of clove extract contained phenolics, flavonoids, and tannins at (28.53 ± 0.00) mg/g, (0.38 ± 0.00) mg/g, and (0.15 ± 0.00) %, respectively. The diameter of the clove extract inhibition zone was significantly different (P < 0.05) in all treatments. The Scanning Electron Microscopy (SEM) result presents that the clove extract can alter the V. alginolyticus cell morphology. The dietary supplementation of clove powder improves the survival rate significantly higher (P<0.05) post-challenge test. The conclusion of this research is that clove extract has antibacterial activity that can inhibit growth and cause cell morphological damage to V. alginolyticus. The application of clove powder at a dose of 15g kg-1 was able to improve the survival value which was a higher post-challenge test. Keyword: antibacterial activity, clove, grouper, phytochemical, Vibrio alginolyticus
Dietary supplementation of Bacillus sp. NP5 and dayak onion simplicia powder Eleutherine bulbosa (Mill.) Urb. for the prevention of Aeromonas hydrophila in catfish Clarias sp. Sudrajat, R Herman; Yuhana, Munti; Widanarni, Widanarni; Julie Ekasari; Afiff, Usamah
Jurnal Akuakultur Indonesia Vol. 22 No. 2 (2023): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.22.2.134-146

Abstract

Aeromonas hydrophila is the main causative agent of ulcerative disease in catfish and causes considerable economic losses to Indonesian aquaculture. This study evaluates the prebiotic activity and the effect of feed supplementation of dayak onion simplicia powder (DOSP) on the immune response and survival of catfish infected with A. hydrophila. Five doses (0.20, 0.25, 0.30, 0.35, and 0.40 g/mL) of DOSP were tested in vitro to assess the prebiotic activity score. The results showed that a dose of 0.20 g/mL gave a significantly (P<0.05) higher probiotic stimulation value than other doses. In the in vivo test, the study used a completely randomized design with five treatments, namely simplicia (DOSP 20 g/kg), probiotic (PRO, Bacillus sp. NP5 108 CFU/mL, 1% v/w), combination (PRO+DOSP), and control (positive and negative). Fish were reared for 45 days and fed three times a day. On day 46, fish from all treatments, except negative control, were infected with an A. hydrophila dose of 106 CFU/mL injected intramuscularly. The results showed that the combination treatment (PRO+DOSP) gave better total erythrocytes, hemoglobin, hematocrit, total leukocytes and phagocytosis activity than probiotics, DOSP, and control. Administering the combination (PRO+DOSP) can reduce the total number of A. hydrophila lower than the probiotic, DOSP, and control treatments. In addition, the survival rate of catfish in the combined treatment (PRO+DOSP) was significantly (P<0.05) higher than probiotics, DOSP, and control. The results of this study can be a helpful reference and application for the early prevention of A. hydrophila infection. Keywords: aquaculture, Bacillus sp. NP5, dayak onion, probiotics, simplicia powder ABSTRAK Aeromonas hydrophila adalah penyebab utama penyakit bercak merah pada budidaya ikan lele dan menyebabkan kerugian ekonomi cukup besar pada akuakultur Indonesia. Penelitian ini bertujuan untuk mengevaluasi aktivitas prebiotik serbuk simplisia bawang dayak (SSBD) dan pengaruh penambahan kombinasi probiotik Bacillus sp. NP5 dan SSBD pada pakan terhadap respons imun dan kelangsungan hidup ikan lele yang diinfeksi A. hydrophila. Lima dosis (0,20, 0,25, 0,30, 0,35, dan 0,40 g/mL) SSBD diuji secara in vitro untuk menilai skor aktivitas prebiotik. Hasil penelitian menunjukkan bahwa dosis 0,20 g/mL memberikan nilai stimulasi probiotik signifikan (P<0,05) lebih tinggi dibandingkan dosis lainnya. Pada uji in vivo, penelitian menggunakan rancangan acak lengkap terdiri dari lima perlakuan, yaitu simplisia (SSBD, serbuk simplisia bawang dayak 20 g/kg), probiotik (PRO, Bacillus sp. NP5 108 CFU/mL, 1% (v/w), kombinasi (PRO+SSBD), dan kontrol (positif dan negatif). Ikan dipelihara selama 45 hari dan diberi pakan tiga kali sehari. Pada hari ke 46, ikan pada semua perlakuan, kecuali kontrol negatif, di infeksi A. hydrophila dosis 106 CFU/mL secara intramuskular. Hasil penelitian menunjukkan bahwa kombinasi (PRO+SSBD) memberikan total eritrosit, hemoglobin, hematokrit, total leukosit dan aktivitas fagositosis lebih baik dibandingkan probitoik, SSBD, dan kontrol. Kombinasi (PRO+SSBD) mampu menekan total A. hydrophila lebih rendah dibandingkan probiotik, SSBD, dan kontrol. Selain itu, tingkat kelangsungan hidup ikan lele pada perlakuan kombinasi (PRO+SSBD) signifikan (P<0,05) lebih tinggi dibandingkan probitoik, SSBD, dan kontrol. Hasil penelitian ini bisa menjadi referensi dan aplikasi yang efektif untuk pencegahan dini infeksi A. hydrophila. Kata kunci: akuakultur, Bacillus sp. NP5, bawang dayak, probiotik, serbuk simplisia
Evaluation of single and multispecies probiotic applications for the prevention of Motile Aeromonads Septicaemia in gourami, Osphronemus gourami Tsani Untsa, Agista; Widanarni, Widanarni; Sukenda, Sukenda; Afiff, Usamah; Yuhana, Munti
Jurnal Akuakultur Indonesia Vol. 23 No. 1 (2024): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.23.1.56-70

Abstract

The aim of this study was to evaluate single and multispecies probiotic applications for the prevention of Motile Aeromonads Septicaemia (MAS) disease in gourami, Osphronemus gourami. The experiment consisted of the in vitro inhibition test and the in vivo application of probiotics in gourami. The in vivo assay, consisted of five treatments and five replicates, namely: negative control (K-); positive control (K+); (B) fish fed with supplementation of 1% (v/w) probiotic Bacillus NP5 RifR cells and challenged with Aeromonas hydrophila RifR; (L) fish fed with supplemention of probiotic Lactobacillus plantarum CipR 1% (v/w); challenged with A. hydrophila RifR; (BL) fish fed with mixture supplementation of Bacillus NP5 RifR 0.5% (v/w)+L. plantarum CipR 0.5%; infected with A. hydrophila RifR. Fish (29.57 ± 1.00 g) were reared in a 45 L volume aquaria with a rearing density of 10 fish/m3 for 50 days, with feeding trial three times a day. The challenge test was conducted by intramuscular injection with pathogenic A. hydrophila RifR cells (106 CFU/mL) on day 41st. In vitro test results showed that single and multispecies probiotics significantly (P<0.05) inhibited A.hydrophila RifR. In vivo experiment showed that probiotic supplementation treatments improved the growth performance, and microbiota diversity in the gut. The immune responses, fish resistance to A. hydrophila RifR and gourami survival rate in all treatments of supplemented feed were significantly higher compared to the positive control. The best treatment, multispecies probiotics significantly (P<0.05) improved the survival of gourami 96.67% post infection with A. hydrophila RifR. Keywords: A. hydrophila, Bacillus NP5, L. plantarum, O. gourami, probiotic ABSTRAK Penelitian ini bertujuan untuk mengevaluasi aplikasi probiotik tunggal dan multispesies dalam pencegahan penyakit Motile Aeromonad Septicaemia (MAS) yang disebabkan oleh Aeromonas hydrophila pada ikan gurami (Osphronemus gourami). Penelitian terdiri atas uji penghambatan in vitro bakteri probiotik tunggal dan multispesies terhadap A. hydrophila RifR, dan uji in vivo aplikasi probiotik untuk gurami. Uji in vivo, terdiri dari lima perlakuan dan lima ulangan, yaitu: kontrol negatif (K-) kontrol positif (K+); (B) ikan dengan pakan suplementasi Bacillus NP5 RifR 1% dan diinfeksi A. hydrophila RifR; (L) pakan komersial dengan penambahan probiotik L. plantarum CipR 1% dan infeksi A. hydrophila RifR; (BL) ikan dengan suplementasi campuran Bacillus NP5 RifR 0.5% dan L. plantarum CipR 0.5% serta diinfeksi A. hydrophila RifR. Benih gurami (29.57 ± 1.00 g) dipelihara di dalam akuarium bervolume 45 L dengan kepadatan 10 ekor/m3 selama 50 hari, dengan pakan bersuplemen probiotik 3 kali sehari. Uji tantang dilakukan dengan menginjeksikan suspensi sel patogen A. hydrophila RifR (106 CFU/mL) secara intramuscular pada hari ke 41. Hasil uji in vitro menunjukkan bahwa probiotik tunggal dan multispesies dapat menghambat pertumbuhan sel A.hydrophila RifR di organ hati dan ginjal secara signifikan (P<0.05). Hasil uji in vivo menunjukkan perlakuan aplikasi probiotik tunggal dan multispesies mampu meningkatkan kinerja pertumbuhan, keragaman mikrobiota usus. Respons imunitas, resistansi terhadap A. hydrophila RifR dan kelangsungan hidup gurami di semua perlakuan pakan bersuplemen probiotik secara signifikan lebih tinggi dibandingkan kontrol positif. Perlakuan terbaik adalah probiotik multispesies meningkatkan kelangsungan hidup ikan sebesar 96.67% secara signifikan (P<0.05) pascainfeksi A. hydrophila RifR. Kata kunci: A. hydrophila, Bacillus sp. NP5, L. plantarum, O. goramy, probiotik
Co-Authors , Alimuddin , Rahman, , Achmad Farouq Ade Dwi Sasanti Adna Sumadikarta ADNI OKTAVIANA Afif, Usamah Afiff , Usamah Agus Alim Hakim Aldy Mulyadin Aliati Iswantari Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Angela M Lusiastuti ANGELA MARIANA LUSIASTUTI Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Zubaidah Anja Meryandini Annisa Astri Anggraeni Arief Muhammad Arini Resti Fauzi Aris Tri Wahyudi Ayi Santika Ayi Santika Bako, Surandha Claritha Madonsa Claritha Madonsa Darna Andrian Ramadhan DEDI JUSADI Destianingrum, Nurin Dalilah Ayu Dinamella Wahjuningrum Eddy Supriyono Efianda, Teuku Reza Emei Widiyastuti Enang Harris Enang Harris Enang Harris Endang Susianingsih Endang Susianingsih Endang Susianingsih Engelhaupt, Martin Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Faoziyatunnisa, Nurul Fauzi, Arini Resti Filibertus Tantio Firdausi, Amalia Putri Fitriana Nazar Gustilatov, Muhamad Hamida Pattah Hamsah Hamsah Hary Krettiawan Hasan Nasrullah Hasan Nasrullah Hermawaty Abubakar Hessy Novita Hessy Novita HORATH, THOMAS Iis Sumartini Iman Rusmana Ince Ayu Khairana Kadriah Ince Ayu Khairana Kadriah Ince Ayu Khairani Kadriah Inem Ode Inka Destiana Sapitri Irzal Effendi Istiqomah, Amalia Jr., Muhammad Zairin Julie Ekasari Ketut Sugama Ketut Sugama Khasanah, Noviati Rohmatul KURT HANSELMANN Kustiariyah Tarman La Ode Baytul Abidin La Ode Muhammad Arsal Laely Nuzullia Lili Sholichah Lilik Setiyaningsih Linuwih Aluh Prastiti M. Zairin Junior Majariana Krisanti Marini Wijayanti Mia Setiawati Mira Mawardi Muhammad Arif Mulya Muhammad Zairin Jr. Muharijadi Atmomarsono Nasrullah, Hasan Neltje Nobertine Palinggi Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nuri Kamaliah, Syarifah Nuzullia, Laely Ode, Inem Pariakan, Arman Putra, The Best Akbar Esa Putri Shandra Ramhirez Ramhirez, Putri Shandra Ranta Sumadi Rasidi Rasidi Rasidi, Rasidi Ricko Reynalta Rini, Adityawati Fajar Ronald Kriston Sauttua Nainggolan Rr. Bellya Anasti Maharani S.H. Dwinanti Salamah Salamah Sefti Heza Dwinanti Soko Nuswantoro Sri Hariati Sri Nuryati Sudrajat, R Herman Sugeng Santoso Sukenda . Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Suleman, Gabriella Augustine Syamsiar, Syamsiar Tambun, Andreas Tri Heru Prihadi Tsani Untsa, Agista Usman Usman Usman Usman UTUT WIDYASTUTI WAODE MUNAENI WAWAN ABDULLAH SETIAWAN Wicaksono, Baref Agung WIDANARNI WIDANARNI Woro Nur Endang Sariati Yanti Inneke Nababan Yunarty Yunarty Yusli Wardiatno Zakki Zainun Zulfani, Anisa