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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Teknologi Perikanan dan Kelautan Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Akuakultur Rawa Indonesia ILMU KELAUTAN: Indonesian Journal of Marine Sciences Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Omni-Akuatika Proceeding Simposium Nasional Kelautan dan Perikanan Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Media Akuatika: Jurnal Ilmiah Jurusan Budidaya Perairan Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Makara Journal of Science Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine

MUNTI YUHANA

Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan - IPB University

Author-ID : 433680

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Library & Information Science Medicine & Pharmacology Social Sciences Veterinary

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GROWTH, IMMUNITY, AND RESISTANCE OF CATFISH (Clarias sp.) REARED IN BIOFLOC SYSTEM SUPPLEMENTED WITH Bacillus NP5 AGAINST Aeromonas hydrophila INFECTION Suleman, Gabriella Augustine; Widanarni, Widanarni; Yuhana, Munti; Afiff, Usamah
Indonesian Aquaculture Journal Vol 19, No 1 (2024): (June, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.1.2024.45-56

Catfish Clarias sp. is one of the leading commodities in demand and has economic value. Low survival due to cannibalism and disease is a serious problem. To overcome these problems is application of bioflocs and probiotics Bacillus NP5. This study aimed to analyze the appropriate dose of Bacillus NP5 probiotic addition to the biofloc system to improve the health status, growth performance, water quality, and resistance to A. hydrophila. The catfish fry (Clarias sp.) with an average weight of 1.79±0.05 g was used in the experiment for 40 days, reared in tanks with the dimension of 60×30×35 cm3. The experiment applied a completely randomized design (CRD) of five treatments with three replicates, namely KN (negative control), KP (positive control), BFT (biofloc application without probiotic bacteria), BFT4 (biofloc application with Bacillus NP5 104 CFU mL-1), BFT6 (biofloc application with Bacillus NP5 106 CFU mL-1). Treatments tested were challenged with A. hydrophila density 104 CFU mL-1 by immersion, except the negative control. After 40 days of rearing, biofloc and Bacillus NP5 treatments had a significant effect (P < 0.05) on growth performance, immune response, water quality, total bacteria in water and the gut compared to the control treatment (P < 0.05). In addition, total A. hydrophila in liver, kidney and water were lower (P < 0.05) in BFT4 and BFT6 treatments than the control. The conclusion of this study is that the bioflocs supplemented with Bacillus NP5 improved the growth performance, immune response and resistance of catfish to A. hydrophila infection.

COMMERCIAL HERBAL ADMINISTRATION FOR PREVENTING Vibrio parahaemolyticus INFECTION IN VANNAMEI SHRIMP (Litopenaeus vannamei) Putra, The Best Akbar Esa; Widanarni, Widanarni; Wahjuningrum, Dinamella; Yuhana, Munti
Indonesian Aquaculture Journal Vol 19, No 1 (2024): (June, 2024)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.1.2024.11-23

Vibrio parahaemolyticus is one of the pathogens in crustaceans that can cause mass death in vannamei shrimp farming. This study aimed to evaluate the effect of administering the commercial herbal supplement Phycurma Aquatic (PA) through feeding at different doses to prevent V. parahaemolyticus infection in vannamei shrimp. This study used a completely randomized design (CRD) consisting of five treatments and three replications, which are negative control group, positive control group, and PA at doses of 2.5, 5.0, and 7.5 mL kg-1 feed. Shrimps were reared for 30 days and fed five times a day. On the 31th day, shrimps were challenged with V. parahaemolyticus at a dose of 105 CFU mL-1, except for the negative control group. The results showed that the administration of 5.0 mL kg-1 of PA in the feed gave the best growth performance (P<0.05) compared to other treatments. The administration of 5.0 mL kg-1 PA in feed also enhanced shrimp health status and significantly increased the total hemocyte count, phagocytic activity, respiratory burst, and phenoloxidase. Furthermore, the administration of PA also increased antioxidant activity, reduced malondialdehyde levels, decreased V. parahaemolyticus population in the intestine, and reduced hepatopancreas tissue damage. Moreover, the survival rate of vannamei shrimp before and after the challenge test in the treatment group with a dose of 5.0 mL kg-1 of PA was significantly higher (P<0.05) compared to other treatments.

Selection of potential probiotic candidate bacteria from seawater and shrimp pond sediments for controlling the pathogenic Vibrio parahaemolyticus Zulfani, Anisa; Yuhana, Munti; Sukenda, Sukenda; Afif, Usamah
Jurnal Akuakultur Indonesia Vol. 24 No. 1 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.1.10-20

Vibrio parahaemolyticus is one of the pathogenic bacteria that cause vibriosis. V. parahaemolyticus strain that expresses the PirA and PirB toxins is the main causative agent of Acute Hepatopancreatic Necrosis Disease which causes necrosis (cell death) suddenly in the hepatopancreatic organs of shrimp. The environmentally friendly approach to prevent bacterial infections in shrimp is through the application of probiotics. Probiotic isolates originated from the same environment as the host and pathogen were expected to have a better adaptation and competition capability. Probiotic selection begins with the process of searching for and screening the potential probiotic candidates. This study aims to obtain isolates and characterize the potential probiotic bacteria isolated from seawater and pond sediments as an inhibition effort V. parahaemolyticus. The research was conducted experimentally using a completely randomized design with 3 treatments and 2 replicates in the in vitro antagonistic test as well as the in vivo non-pathogenicity test. The results of the study successfully obtained 37 probiotic candidate isolates with 10 isolates having the greatest enzyme activity. LAZ-2 (Acinetobacter radioresistens) isolated from seawater and SAZ-22 (Fusobacterium varium) isolated from pond sediment have been selected as probiotic candidate bacteria that have beneficial enzymatic activities. Both isolates were capable of inhibiting the growth of the pathogenic V. parahaemolyticus cells population and both were not pathogenic to vannamei shrimp. Keywords: probiotic, screening, vannamei shrimp, Vibrio parahaemolyticus ABSTRAK Vibrio parahaemolyticus merupakan salah satu bakteri patogen penyebab penyakit vibriosis. V. parahaemolyticus strain tertentu yang mengekspresikan toksin PirA dan PirB merupakan agen penyebab utama Acute Hepatopancreatic Necrosis Disease yang menyebabkan nekrosis (kematian sel) secara tiba-tiba pada organ hepatopankreas udang. Pendekatan yang ramah lingkungan untuk mencegah penyakit infeksi bakterial pada udang adalah melalui aplikasi probiotik. Isolat-isolat probiotik yang berasal dari lingkungan yang sama dengan inang dan patogen akan memiliki suatu kemampuan adaptasi dan kompetisi yang lebih baik dari yang lainnya. Seleksi probiotik diawali dengan proses pencarian dan skrining kandidat probiotik yang potensial. Penelitian ini bertujuan untuk mendapatkan isolat dan mengkarakterisasi bakteri probiotik potensial yang diisolasi dari air laut dan sedimen tambak sebagai upaya penghambatan V. parahaemolyticus. Penelitian dilakukan secara eksperimental menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan 2 kali ulangan pada uji antagonistik in vitro dan uji non-patogenisitas. Hasil penelitian berhasil mendapatkan 37 isolat kandidat probiotik dengan 10 isolat yang memiliki aktivitas enzim terbesar. LAZ-2 (Acinetobacter radioresistens) yang diisolasi dari air laut dan SAZ-22 (Fusobacterium varium) yang diisolasi dari sedimen tambak terpilih sebagai bakteri kandidat probiotik yang memiliki aktivitas enzim yang menguntungkan. Kedua isolat tersebut mampu menghambat pertumbuhan populasi sel patogen V. parahaemolyticus dan keduanya tidak bersifat patogenik terhadap udang vaname. Kata kunci: probiotik, seleksi, udang vaname, Vibrio parahaemolyticus

Existing of White Spot Syndrome Virus (WSSV), Taura Syndrome Virus (TSV) and Infectious Hypodermal Haematopoitic Necrosis Virus (IHHNV) in White Shrimp Litopenaeus vannamei Reared at Intensive Tambak System in Bakauheni, Lampung Selatan Dwinanti, S.H.; Yuhana, Munti
Jurnal Akuakultur Indonesia Vol. 8 No. 2 (2009): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Development of white shrimp, Litopenaeus vannamei culture to increase production yield should take attention in prevention of viral outbreak which is a main factor caused harvesting failure. Viruses that usually infect shrimp are white spot syndrome virus (WSSV), taura syndrome virus (TSV) dan infectious hypodermal hematopoietic necrosis virus (IHHNV). To prevent virus infection and reduce risk of fail to harvest, an early warning and observation system of availability of pathogen should be taken in order to know the shrimp condition during culture process. This study was performed to examine existing of WSSV, TSV and IHHNV in shrimp reared in tambak by PCR and histopathology methods. Samples were taken every two weeks from tambak culture at Bakauheni, Penengahan, Lampung Selatan. Generally, WSSV, TSV, and IHHNV were found in shrimp from tambak and water around tambak. Virus infection was first detected in shrimp derived from tambak on 66 days after crop and still exists until 107 days after crop. Hepatopancreatic cell disorder caused by the three viruses were found in cell nuclei that became bigger, necrosis in cytoplasm and inclusion body at nuclei. The existing of virus in outside of tambak indicated that virus was horizontally transmitted. Keywords: WSSV, TSV, IHHNV, white shrimp ABSTRAK Perkembangan budidaya udang vaname Litopenaeus vannamei dalam usaha meningkatkan hasil produksi harus memperhatikan keberadaan penyakit viral yang menjadi penyebab utama kegagalannya. Virus yang biasa menyerang vaname antara lain white spot syndrome virus (WSSV), taura syndrome virus (TSV) dan infectious hypodermal hematopoietic necrosis virus (IHHNV). Untuk mengantisipasi penyebaran virus dan mengurangi resiko kegagalan produksi diperlukan usaha pencegahan dengan melakukan peringatan dini (early warning) dan pemantauan tambak terhadap keberadaan patogen tersebut selama masa budidaya. Penelitian ini dilakukan untuk mengetahui keberadaan WSSV, TSV dan IHHNV yang menginfeksi udang vaname dengan analisa polymerase chain reaction (PCR) dan histopatologis. Pengambilan sampel dilakukan sebanyak delapan kali setiap dua minggu di tambak intensif udang vaname Litopenaeus vannamei di Bakauheni, Penengahan, Lampung Selatan. Secara umum, WSSV, TSV dan IHHNV telah ditemukan pada tambak serta di perairan sekitar lokasi tersebut. Infeksi virus pertama kali terdeteksi di tambak ketika udang berumur 66 hari setelah penebaran dan terdeteksi pula pada waktu pengamatan selanjutnya sampai udang berumur 107 hari setelah penebaran. Kerusakan akibat serangan ketiga jenis virus tersebut terjadi pada bagian inti sel yang mengalami pembesaran, nekrosis pada sitoplasma dan badan inklusi yang menekan inti sel. Pola transmisi virus yang terjadi adalah secara horizontal yang didukung oleh terdeteksinya virus tersebut pada udang di luar tambak. Kata kunci : WSSV, TSV, IHHNV, udang vaname

Biocontrol agents in aquaculture: Production and their application Yuhana, Munti
Jurnal Akuakultur Indonesia Vol. 9 No. 1 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The use of biocontrol agents have become commonly accepted as a 'natural weapon' in aquaculture, either as an ecological bioremediator for low environmental quality, as well as the health promoter for cultured organisms. Biocontrol agents, which consist of beneficial microorganisms, are commonly applied as biosupplements in feeds. They are potential in replacing the use of antibiotic in inhibiting the pathogenic organisms. Therefore, their application has revealed the potential as an effective strategy to reduce the over use of antibiotics in controlling the pathogenic agents, avoid the spreading the drug resistance, or environmental deterioration of the negative effect by killing useful microorganisms. The development of suitable technology for microbial agents production, viability and stability, is a key area of research for industrial production. Production of biocontrol agents should be based on the microbial criteria, and the ability to withstand stress during processing and storage of products is crucial. This review makes an overview of biocontrol agents selection studies including techniques for isolation/identification, selection, production and its application in order to be accepted as a valuable product in aquaculture. Key words: Biocontrol agents, beneficial microorganisms, aquaculture. ABSTRAK Pemanfaatan agen biokontrol telah dapat diterima secara luas di dunia akuakultur sebagai senjata alami, baik sebagai bioremediator ekologis untuk memperbaiki kualitas lingkungan yang rendah maupun sebagai promoter/pendukung kesehatan untuk organisme-organisme yang dibudidayakan. Agen-agen biokontrol yang terdiri atas berbagai mikroorganisme yang menguntungkan umumnya diaplikasikan sebagai biosuplemen dalam pakan. Mikroorganisme tersebut potensial dalam menggantikan penggunaan antibiotik dalam proses penghambatan terhadap organisme patogenik. Oleh karena itu, aplikasinya yang sangat potensial dapat berguna sebagai strategi efektif untuk mereduksi penggunaan berlebih dari antibiotik dalam pengendalian agen patogenik, mencegah penyebaran sifat resistensi terhadap obat-obatan, atau mencegah kerusakan lingkungan dari kematian mikroorganisme yang bermanfaat. Pengembangan teknologi yang sesuai untuk produksi, viabilitas dan stabilitas dari agen biokontrol tersebut, merupakan kunci dari penelitian untuk produksi masal pada skala industri. Proses produksi dari agen biokontrol harus berdasar pada kriteria mikrobiologis. Sifat-sifat ketahanannya terhadap stress selama pemrosesan maupun penyimpanan produk adalah penting. Pada makalah ini disajikan teknik-teknik untuk isolasi, identifikasi, produksi dan aplikasi agen biokontrol untuk dapat diterima sebagai produk yang bernilai ekonomis dalam akuakultur. Kata kunci: Agen biokontrol, mikroorganisme yang menguntungkan, akuakultur.

Efficacy of DNA vaccine encoding koi herpesvirus glycoprotein GP-25in common carp juvenile by immersion Nuswantoro, Soko; Alimuddin, .; Yuhana, Munti; Santika, Ayi; Nuryati, Sri; Zainun, Zakki; Mawardi, Mira
Jurnal Akuakultur Indonesia Vol. 11 No. 1 (2012): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Koi herpesvirus (KHV) is a herpesvirus that particularly infects and causes mass mortality to koi and common carp. Therefore, the protection of common carp from KHV infection is urgently needed. In this study, we developed an application of DNA vaccine encoding KHV glycoprotein-25 by immersion method to increase survival of common carp against KHV infection. A total of 400 common carp juveniles at 30-day-old were immersed in 1-L water containing 1.3×108CFU/mL of the killed Escherichia coli cells carrying DNA vaccine. Three frequencies and three duration of fish immersion were tested, namely: 1×30 minutes, 1×60 minutes, 1× 90 minutes, 2×90 minutes and 3×90 minutes by interval of 24 hours. Reversetranscription polymerase chain reaction analysis showed that DNA vaccine was successfully expressed in the vaccinated fish. Fish at twenty eight days post vaccination were challenged by injecting 10-4 mL of KHV per fish. The result showed that vaccination by 1×30 minutes immersion allowed 61% of fish survived, and this was significantly higher (p<0.05) compared to control (without vaccination), but it was similar among vaccination treatments (p>0.05). The relative percent survival of vaccinated fish were also similar among treatments (p>0.05). DNA vaccination has increased fish survival about two fold higher compared to unvaccinated fish control (26.67%). Thus, DNA vaccination was effectively delivered by immersion for 1×30 minutes, and this technique can be useful to level up the resistance of common carp juveniles against KHV infection. Keywords: DNA vaccine, KHV, glycoprotein, immersion, common carp

Survival of common carp carrying Cyca-DAB1*05 post-challenged with Aeromonas hydrophila Arsal, Laode Muhammad; Yuhana, Munti; Nuryati, Sri; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 13 No. 2 (2014): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Blood parameters are considered as important indicators to diagnose fish health status. This study was performed to observe blood profiles including total erythrocytes, hemoglobin concentration, hematocrite, total leukocytes and differential leukocytes, and survival of common carp Cyprinus carpio infected by Aeromonas hydrophila. Fish were divided into two groups: the 3rd generation of common carp carrying fish carrying Cyca-DAB1*05 of major histocompatibility complex II molecular marker, and fish without the marker as control treatment. histocompatibility complex II molecular marker, and fish without the marker as control treatment. Common carp 3rd generation was produced by crossing among second generation of fish carrying the Cyca-DAB1*05 marker. Each fish was injected intramuscularly by 0.1 mL of 108 cfu/mL A. hydrophila. Challenge test was conducted for 14 days and blood was collected at day-0, three, seven, and 14. The results of this study showed that erythrocytes, hemoglobin and hematocrite concentrations of common carp carrying the molecular marker at post challenge with A. hydrophila were higher (P<0.05) compared to control fish. The blood profiles were highly correlated to survival of fish. Survival of fish that carrying the molekuler marker was about two point six fold higher than those of control fish. Keywords: Cyprinus carpio, Aeromonas hydrophila, Cyca-DAB1*05, molecular marker ABSTRAK Gambaran darah merupakan indikator penting untuk mendiagnosa penyakit ikan. Penelitian ini dilakukan untuk meguji gambaran darah ikan mas Cyprinus carpio setelah diinfeksi dengan bakteri Aeromonas hydrophila yang meliputi total sel darah merah, konsentrasi hemoglobin, hematokrit, total sel darah putih, dan diferensial leukosit, serta sintasannya. Ikan mas yang digunakan terdiri atas ikan mas generasi ketiga yang membawa marka molekuler Cyca-DAB1*05 dari kelompok major histocompatibility complex II dan ikan mas tanpa marka sebagai kontrol. Ikan mas generasi ketiga merupakan keturunan persilangan antarikan mas generasi kedua yang mempunyai marka Cyca-DAB1*05. Masing-masing ikan diinfeksikan A. hydrophila secara intramuskuler pada dosis 0,1 mL, kepadatan 108 cfu/mL. Uji tantang dilakukan selama 14 hari, dan sampel darah ikan diambil pada hari ke-0, tiga, tujuh dan 14. Hasil uji tantang menunjukkan bahwa total sel darah merah, konsentrasi hemoglobin dan hematokrit pada ikan mas yang membawa marka molekuler lebih tinggi dibandingkan ikan mas tanpa marka (P<0,05). Hasil uji gambaran darah berkorelasi tinggi dengan tingkat kelangsungan hidup ikan mas. Tingkat kelangsungan hidup ikan mas yang membawa marka molekuler Cyca-DAB1*05 lebih tinggi hingga dua koma enam kali daripada ikan mas kontrol. Kata kunci: Cyprinus carpio, Aeromonas hydrophila, Cyca-DAB1*05, marka molekuler

Potency of sponge-associated bacteria producing bioactive compounds as biological control of vibriosis on shrimp Rini, Adityawati Fajar; Yuhana, Munti; Wahyudi, Aris Tri
Jurnal Akuakultur Indonesia Vol. 16 No. 1 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The aims of this study were to obtain sponge-associated bacteria as biocontrol to inhibit vibriosis in vitro and in vivo, to identify the bacterial isolates based on 16S-rRNA gene, and to detect the presence of nonribosomal peptide synthetase (NRPS), and polyketide synthase (PKS) genes to prove its ability of bioactive compounds synthesis. Aaptos sp. and Hyrtios sp. sponges were collected from Pramuka Island, Jakarta. The isolation using sea water complete (SWC) and zobel marine agar (ZMA) medium obtained 174 isolates. A total 69 isolates were screened successfully based on their antibacterial activity. 47 isolates showed negative haemolysis through hemolytic assays. The pathogenicity test used twelve selected isolates that have a broad spectrum of antibacterial activity and haemolysis negative. The result of pathogenicity test showed that 12 isolates were not pathogenic to the shrimp post larvae with no significantly different (P>0.05) between treatment and negative control. Results of challenge test with Vibrio harveyi have a significant difference survival (70±5.0–90±0.0%) (P<0.05) compared with positive control (38.3±2.9%). Genetic analysis based on 16S-rRNA revealed the groups of three genera belonged to Pseudomonas, Staphylococcus, and Alcaligenes. Based on amplification of NRPS and PKS genes, four bacterial isolates have been detected to have only NRPS gene, one isolate has only PKS, and one isolate has both genes. The results indicate that the potency of six sponge-associated bacteria as bioactive compounds producers. Keywords: NRPS, PKS, anti-vibriosis, Pacific white shrimp ABSTRAK Penelitian ini bertujuan untuk memperoleh isolat bakteri asosiasi spons yang mempunyai kemampuan dalam menghambat vibriosis secara in vitro, in vivo dan mendeteksi gen 16S-rRNA, nonribosomal peptide synthase (NRPS) serta polyketide synthase (PKS) untuk memastikan kemampuan mensintesis senyawa bioaktif. Spons Aaptos sp. dan Hyrtios sp. berhasil dikoleksi dari perairan Pulau Pramuka, Kep. Seribu Jakarta. Isolasi bakteri dengan menggunakan media sea water complete (SWC) dan zobel marine agar (ZMA) diperoleh 174 isolat. Sebanyak 69 isolat terdeteksi memiliki aktivitas antibakteri. Uji hemolisis menunjukkan 47 isolat adalah hemolisis negatif. Uji patogenisitas menggunakan 12 isolat terpilih yang memiliki spektrum luas dan hemolisis negatif. Hasil uji patogenisitas tehadap 12 isolat menunjukkan bahwa semua isolat tidak bersifat patogen terhadap pascalarva udang vaname. Hal ini dibuktikan dengan sintasan pascalarva udang vaname yang tidak berbeda nyata (P>0,05) dengan kontrol negatif. Hasil uji tantang terhadap Vibrio harveyi diketahui sintasan pascalarva udang vaname (70±5,0–90±0,0%) memiliki perbedaan yang signifikan jika dibandingkan dengan kontrol positif (38,3±2,9%). Berdasarkan analisis sekuen gen 16S-rRNA, menunjukkan bahwa isolat-isolat tersebut memiliki kemiripan dengan genus Pseudomonas, Staphylococcus, dan Alcaligenes. Deteksi gen NRPS dan PKS menggunakan PCR diperoleh empat isolat bakteri memiliki hanya gen NRPS, satu isolat memiliki hanya gen PKS, dan satu isolat memiliki kedua gen NRPS-PKS. Hal ini membuktikan bahwa keenam isolat memiliki potensi sebagai penghasil senyawa bioaktif. Kata kunci: NRPS, PKS, antivibriosis, udang vaname

The evaluation of different levels diets protein for growth performance of Clarias sp. fry cultured in biofloc-based system Khasanah, Noviati Rohmatul; Priyo Utomo, Nur Bambang; Setiawati, Mia; Yuhana, Munti
Jurnal Akuakultur Indonesia Vol. 16 No. 2 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The study was conducted to evaluate the performance of catfish fry grown using different dietary protein levels in the biofloc-base aquaculture system. Experiments using a completely randomized design, consisted of four treatments and three replications. The treatment consisted of: protein 38% (A), 34% protein (B), 30% protein (C), and protein 26% (D). Catfish with initial weight of 0.83±0.01 g and length of 4.64±0.04 cm were cultured in 60 L tank with density of 90 fish each tank for 35 days. Inoculation of heterotrophic bacterial Staphylococcus lentus L1k were performed of 104 CFU/mL ratio of 15 was administrated once a day after two hours feeding in the morning. Feeding was conducted twice a day at 5% of the biomass weight. At the end of trial treatment (D) showed the highest survival rate (88.15±5.25%), the body lenght variance coefficient (9.58±0.51%) and protein retention (39.87±2.77%). Treatment (B) showed the highest growth rate (4.11±0.05%), total length (2.39±0.08 cm), and feed intake (318.76±4.63). Treatment (A) showed the highest feed efficiency (93.65±4.43%) while lowest lipid retention compared to others (22.20±1.20%.). Based on the results, it can be concluded that 34% protein feed (C) can replace 38% protein feed (B) catfish fry size 4−5 cm through biofloc-based system. Keywords: biofloc, fry, growth, protein, Staphylococcus lentus L1k ABSTRAK Penelitian bertujuan mengevaluasi kinerja pertumbuhan benih lele dengan menggunakan kadar protein pakan yang berbeda pada sistem bioflok. Percobaan dilakukan menggunakan rancangan acak lengkap, terdiri atas empat perlakuan dan tiga ulangan. Perlakuan terdiri atas protein 38% (A), protein 34% (B), protein 30% (C), dan protein 26% (D), terdiri atas empat perlakuan dan tiga ulangan. Benih lele dengan berat rata-rata 0,83±0,01 g dan panjang rata-rata 4,64±0,04 cm dipelihara di akuarium berukuran 90×50×40 cm3 dengan padat tebar 90 ekor/akuarium selama 35 hari. Inokulasi bakteri heterotrof berupa Staphylococcus lentus L1k dilakukan pada setiap perlakuan kepadatan 104 CFU/mL. Penambahan sumber karbon berupa molase dengan C/N (Carbon/Nitrogen) rasio 15 diberikan satu kali sehari setelah dua jam pemberian pakan di pagi hari. Pakan diberikan dua kali sehari sebanyak 5% dari berat biomasa. Hasil penelitian menunjukkan pemberian kadar protein pakan berbeda memberikan hasil yang positif. Perlakuan (D) menunjukkan tingkat kelangsungan hidup (88,15±5,25%), koefisien keragaman panjang (9,58±0,51%) dan retensi protein (39,87±2,77%) terbaik. Perlakuan (B) menunjukkan laju pertumbuhan harian (4,11±0,05%), panjang total (2,39±0,08 cm), dan jumlah konsumsi pakan (318,76±4,63) tertinggi. Perlakuan (A) menunjukkan efisiensi pakan (93,65±4,43%) tertinggi namun menunjukkan retensi lemak (22,20±1,20%) terendah. Berdasarkan hasil penelitian disimpulkan bahwa pemberian pakan dengan kadar protein 34% mempunyai performa pertumbuhan yang sama dibandingkan protein 38% pada benih ikan lele berukuran 4−5 yang dipelihara menggunakan sistem budidaya bioflok. Kata kunci: benih lele, protein, pertumbuhan, bioflok, Staphylococcus lentus L1k

Multispecies probiotics applications through feed addition on cultured tilapia to prevent streptococcosis Destianingrum, Nurin Dalilah Ayu; Yuhana, Munti; Lusiastuti, Angela Mariana
Jurnal Akuakultur Indonesia Vol. 16 No. 2 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Streptococcosis is one of the diseases that often arise and can cause death by 100% in tilapia fish farming. This study aimed to determine the most optimal probiotics combination of cells Bacillus cereus P22, Bacillus subtillis ND2 and Staphylococcus lentus L1k through the feed for streptococcosis prevention. The experimental fish with initial body weight of (13.06±0.18 g), were randomly distributed at 25 fish per 60 L water, and reared for 21 days. The fish fed with supplemented feed (except controls) with FR 3%, about three times a day, for 14 days. The treatment of combination probiotics-supplemented feed on, K1 (P22 and ND2, density of 106 CFU/mL), K2 (ND2 and L1k, density of 105 CFU/mL), K3 (P22 and L1k, density of 105 CFU/mL), K4 (P22, ND2 and L1k, density of 106 CFU/mL), the negative and positive control treatment given feed without supplementation. On day 15, the experimental fish, (except negative control) were challenged with Streptococcus agalactiae via intraperitoneal injection 0.1 mL/fish (104 CFU/mL). The results indicated that the best combination was shown by K3 (the combination of Bacillus cereus P22 and Staphylococcus lentus L1k) with cells addition 1% (v/w). The result of K3 survival rate shown 89.33±6.11%, significantly different from the K + and K- wich were 66.67±4.62% and 88.00±8.00%. The highest increased immune value shown by K3 were wich phagocytic index value 64.00±2.65% and respiratory burst activity value 0.07±0.00 OD. Keywords: multispecies probiotics, Oreochormis niloticus, streptococcosis ABSTRAK Streptococcosis merupakan salah satu penyakit yang seringkali muncul dan dapat menyebabkan kematian hingga 100% pada budidaya ikan nila. Penelitian ini bertujuan untuk menentukan kombinasi optimal dari probiotik Bacillus cereus P22, Bacillus subtillis ND2, dan Staphylococcus lentus L1k melalui pakan sebagai upaya pencegahan streptococcosis. Ikan uji berukuran (13,06±0,18 g), secara acak dipelihara dengan kepadatan 25 ekor/akuarium dengan volume air 60 L, dipelihara selama 21 hari dan diberi pakan yang disuplementasi probiotik (kecuali kontrol) dengan perkiraan FR 3% sebanyak tiga kali sehari, selama 14 hari. Perlakuan kombinasi probiotik yang disuplementasi pada pakan yaitu: K1 (P22 dan ND2, kepadatan 106 CFU/mL), K2 (ND2 dan L1k, kepadatan 105 CFU/mL), K3 (P22 dan L1k, kepadatan 105 CFU/mL), K4 (P22, ND2 dan L1k, kepadatan 106 CFU/mL), perlakuan kontrol negatif dan posistif diberi pakan tanpa suplementasi probiotik. Pada hari ke-15, ikan uji (kecuali kontrol negatif) diinjeksi dengan Streptococcus agalactiae dengan kepadatan 104 CFU/mL sebanyak 0,1 mL secara intraperitoneal. Hasil penelitian ini menunjukkan bahwa kombinasi probiotik multispesies terbaik adalah perlakuan K3 (kombinasi Bacillus cereus P22 dan Staphylococcus lentus L1k) dengan dosis 1% (v/w). Nilai kelangsungan hidup K3 didapat 89,33±6,11%, berbeda nyata dengan K+ (66,67±4,62%) dan K- (88,00±8,00%). Nilai respons imun dengan peningkatan tertinggi pada perlakuan K3 dengan nilai indeks fagositik 64,00±2,65% dan aktivitas respiratory burst 0,071±0,00 OD. Kata kunci: probiotik multispesies, Oreochormis niloticus, streptococcosis

Co-Authors , Alimuddin , Rahman, , Achmad Farouq Ade Dwi Sasanti Adna Sumadikarta ADNI OKTAVIANA Afif, Usamah Afiff , Usamah Agus Alim Hakim Aldy Mulyadin Aliati Iswantari Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Angela M Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti ANGELA MARIANA LUSIASTUTI Angela Mariana Lusiastuti Anis Zubaidah Anja Meryandini Annisa Astri Anggraeni Arief Muhammad Arini Resti Fauzi Aris Tri Wahyudi Ayi Santika Ayi Santika Bako, Surandha Claritha Madonsa Claritha Madonsa Darna Andrian Ramadhan DEDI JUSADI Destianingrum, Nurin Dalilah Ayu Dinamella Wahjuningrum Eddy Supriyono Efianda, Teuku Reza Emei Widiyastuti Enang Harris Enang Harris Enang Harris Endang Susianingsih Endang Susianingsih Endang Susianingsih Engelhaupt, Martin Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Faoziyatunnisa, Nurul Fauzi, Arini Resti Filibertus Tantio Firdausi, Amalia Putri Fitriana Nazar Gustilatov, Muhamad Hamida Pattah Hamsah Hamsah Hary Krettiawan Hasan Nasrullah Hasan Nasrullah Hermawaty Abubakar Hessy Novita Hessy Novita HORATH, THOMAS Iis Sumartini Iman Rusmana Ince Ayu Khairana Kadriah Ince Ayu Khairana Kadriah Ince Ayu Khairani Kadriah Inem Ode Inka Destiana Sapitri Irzal Effendi Istiqomah, Amalia Jr., Muhammad Zairin Julie Ekasari Ketut Sugama Ketut Sugama Khasanah, Noviati Rohmatul KURT HANSELMANN Kustiariyah Tarman La Ode Baytul Abidin La Ode Muhammad Arsal Laely Nuzullia Lili Sholichah Lilik Setiyaningsih Linuwih Aluh Prastiti M. Zairin Junior Majariana Krisanti Marini Wijayanti Mia Setiawati Mira Mawardi Muhammad Arif Mulya Muhammad Zairin Jr. Muharijadi Atmomarsono Nasrullah, Hasan Neltje Nobertine Palinggi Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nuri Kamaliah, Syarifah Nuzullia, Laely Ode, Inem Pariakan, Arman Putra, The Best Akbar Esa Putri Shandra Ramhirez Ramhirez, Putri Shandra Ranta Sumadi Rasidi Rasidi Rasidi, Rasidi Ricko Reynalta Rini, Adityawati Fajar Ronald Kriston Sauttua Nainggolan Rr. Bellya Anasti Maharani S.H. Dwinanti Salamah Salamah Sefti Heza Dwinanti Soko Nuswantoro Sri Hariati Sri Nuryati Sudrajat, R Herman Sugeng Santoso Sukenda . Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Suleman, Gabriella Augustine Syamsiar, Syamsiar Tambun, Andreas Tri Heru Prihadi Tsani Untsa, Agista Usman Usman Usman Usman UTUT WIDYASTUTI WAODE MUNAENI WAWAN ABDULLAH SETIAWAN Wicaksono, Baref Agung WIDANARNI WIDANARNI Woro Nur Endang Sariati Yanti Inneke Nababan Yunarty Yunarty Yusli Wardiatno Zakki Zainun Zulfani, Anisa

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