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DETEKSI Vibrio parahaemolyticus MENGGUNAKAN MARKA GEN PirA PADA UDANG VANAME (Litopenaeus vannamei) DENGAN REAL TIME PCR Ronald Kriston Sauttua Nainggolan; Munti Yuhana; Sukenda Sukenda; Woro Nur Endang Sariati
Jurnal Riset Akuakultur Vol 15, No 2 (2020): (Juni, 2020)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Pengujian validitas deteksi Vibrio parahaemolyticus strain AHPND pada udang vaname (Litopenaeus vannamei) dengan metode real time PCR dilakukan melalui tahapan pengujian yang mencakup spesifisitas, sensitivitas, repitabilitas, reproduktivitas, dan uji lapang pada tambak-tambak udang vaname di Indonesia. Uji performa analitik menunjukkan bahwa metode real time PCR dengan penciri gen VpPirA spesifik dalam mendeteksi V. parahaemolyticus strain AHPD dan memiliki sensitivitas hingga 10 kopi µL-1. Nilai cut off metode qPCR primer VpPirA adalah di Ct 39. Metode real time PCR dengan primer spesifik VpPirA juga memiliki nilai repitabilitas dan reproduktivitas yang baik dalam mendeteksi V. parahaemolyticus strain AHPND. Semua pengujian performa analitik metode qPCR telah memenuhi syarat keberterimaan dan dapat digunakan sebagai metode pengujian untuk mendeteksi V. parahaemolyticus strain AHPND. Hasil uji lapang untuk seluruh sampel yang berasal dari tambak udang vaname menunjukkan hasil negatif terinfeksi V. parahaemolyticus strain AHPND. Studi ini menyarankan bahwa metode real time PCR efektif dan valid dalam mendeteksi V. parahaemolyticus strain AHPND.Testing the validity of Vibrio parahaemolyticus AHPND (Vp AHPND) strain detection in whiteleg shrimp (Litopenaeus vannamei) by real-time PCR method was carried out through several stages including specificity, sensitivity, repeatability, reproducibility, and sampling field tests in shrimp ponds in Indonesia. Analytical performance test showed that the use of real-time PCR method with VpPirA primer was effective in detecting Vp AHPND strain and had a sensitivity of up to 10 copies µL-1. The cut off value qPCR method of the primer VpPirA is at Ct 39. The real-time PCR method using VpPirA primer also has good repeatability and reproducibility values in detecting Vp AHPND strain. All analytical performance testings of the qPCR method meet the standard requirements to detect Vp AHPND strain. The result of field tests for all whiteleg shrimp samples from the shrimp ponds revealed negative infection of Vp AHPND. This study suggests that the real time PCR is effective and valid in detecting V. parahaemolyticus AHPND strain.

KARAKTERISASI BAKTERI ANTI QUORUM SENSING (AQS) SEBAGAI PENGHAMBAT VIRULENSI PENYAKIT PADA IKAN LELE DUMBO (Clarias gariepinus) Hessy Novita; Iman Rusmana; Munti Yuhana; Fachriyan Hasmi Pasaribu
Jurnal Riset Akuakultur Vol 10, No 1 (2015): (Maret 2015)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Penyakit bakterial adalah salah satu penyebab kerugian besar di bidang akuakultur. Faktor virulensi bakteri penyakit umumnya diekspresikan oleh gen-gen virulen yang diregulasi dengan sistem Quorum Sensing. Tujuan dari penelitian ini adalah untuk mendapatkan isolat bakteri yang memiliki potensi sebagai Anti Quorum Sensing (AQS) yang dapat menghambat faktor virulensi bakteri patogen penyebab penyakit pada ikan lele dumbo (Clarias gariepinus). Isolasi bakteri dilakukan dari sedimen, organ pencernaan dan air kolam ikan lele dumbo dari Parung, Ciampea, dan Gunung Sindur, Bogor, Jawa Barat. Sebanyak 347 isolat bakteri berhasil diisolasi, dan sebanyak 68 (19%) isolat di antaranya mempunyai aktivitas AQS dengan empat isolat yang berpotensi sebagai bakteri AQS yaitu: TS 1 dan TS 2, TA 23, dan TY 33. Empat isolat tersebut teridentifikasi berdasarkan sekuen 16S rRNA sebagai Bacillus amyloliquefaciens, Lysinnibacillus sphaericus, Lysinnibacillus fusiformis, dan Bacillus cereus dengan persentase kemiripan masing-masing 93%, 99%, dan 100%. Berdasarkan analisa gen AHL (Acyl Homoserine Lactone) laktonase (aiiA), keempat isolat tersebut menghasilkan enzim AHL. Hasil studi ini menunjukkan bahwa isolat bakteri hasil isolasi dari kolam ikan lele dumbo dapat menghambat mekanisme Quorum Sensing bakteri patogen ikan dengan mendegradasi autoinduser-nya yang berupa AHL.

Kinerja Pertumbuhan dan Respons Imun Larva Udang Vaname yang diberi Probiotik Pseudoalteromonas piscicida dan Prebiotik Mannanoligosakarida melalui Bioenkapsulasi Artemia sp. Hamsah Hamsah; Widanarni Widanarni; Alimuddin Alimuddin; Munti Yuhana; Muhammad Zairin Junior
Prosiding Simposium Nasional Kelautan dan Perikanan Vol. 5 (2018): PROSIDING SIMPOSIUM NASIONAL V KELAUTAN DAN PERIKANAN UNHAS
Publisher : Fakultas Ilmu Kelautan dan Perikanan (FIKP), Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (745.734 KB)

Pemberian probiotik, prebiotik, dan sinbiotik merupakan salah satu cara untuk meningkatkan pertumbuhan dan respons imun pada ikan, udang, dan organisme akuatik lainnya. Penelitian ini bertujuan mengevaluasi kinerja pertumbuhan dan respons imun larva udang vaname yang diberi probiotik Pseudoalteromonas piscicida (1Ub), prebiotik mannanoligosakarida (MOS), dan sinbiotik (kombinasi probiotik 1Ub dan prebiotik MOS) melalui bioenkapsulasi Artemia sp. Bioenkapsulasi dilakukan dengan cara menambahkan probiotik 1Ub konsentrasi 106 CFU/mL, prebiotik MOS 12 mg/L, dan sinbiotik (kombinasi 106 CFU/mL 1Ub dengan 12 mg/L MOS) pada media pemeliharaan Artemia sp. selama 4 jam. Pemberian Artemia sp. hasil bioenkapsulasi ke larva udang dilakukan selama 13 hari (Mysis3 sampai PL12). Pertumbuhan panjang dan bobot tubuh larva udang vaname diamati pada awal dan akhir penelitian, sedangkan rasio RNA/DNA, aktivitas enzim pencernaan, kelangsungan hidup, jumlah total bakteri, dan respons imun larva udang meliputi total hemosit (THC), aktivitas phenoloxidase (PO), dan aktivitas respiratory burst (RB) dianalisa pada akhir penelitian. Hasil penelitian menunjukkan laju pertumbuhan harian (DGR), panjang mutlak, rasio RNA/DNA, aktivitas enzim, kelangsungan hidup, jumlah total bakteri, dan respons imun pada larva udang yang diberi probiotik, prebiotik, dan sinbiotik berbeda nyata (p˂0,05) dibandingkan dengan kontrol. Pemberian sinbiotik menunjukkan hasil terbaik dengan DGR (24.39±0.31% per hari), panjang mutlak (13.00±0.50 mm), rasio RNA/DNA (0.6369±0.0094), aktivitas enzim pencernaan (protease 0.033±0.0007; lipase 0.047±0.0010; amilase 0.853±0.008; mananase 0.148±0.004 U/mL/menit), kelangsungan hidup (92.67±1.26%), jumlah total bakteri (6.7 x 107 CFU/0.1g larva), THC (7.6 x 106 sel/mL), aktivitas PO (0.19±0.002 OD 490 nm), dan aktivitas RB (0.67±0.028 OD 630 nm) yang lebih tinggi dibandingkan perlakuan lainnya dan kontrol. Kata Kunci: probiotik, prebiotik, sinbiotik, Artemia sp., udang vaname.

PERFORMA PERTUMBUHAN DAN KETAHANAN STRES STADIA AWAL UDANG GALAH Macrobrachium rosenbergii YANG DIBERI Bacillus sp. Munti Yuhana; Darna Andrian Ramadhan; Hary Krettiawan; Usamah Afiff
Jurnal Teknologi Perikanan dan Kelautan Vol 12 No 1 (2021): MEI 2021
Publisher : Fakultas Perikanan dan Ilmu Kelautan

The study aimed to evaluate the effect of the water application of probiotic Bacillus sp. DMP13 on the growth and stress response of giant freshwater prawn larvae Macrobrachium rosenbergii. The larvae used in this experiment were 1-day-old larvae post-hatching with an average body weight of 2.7Â±0.6 mg and an average length of 2.1Â±0.1 mm. The larvae were reared in 2 L plastic tanks with a density of 100 L-1 for 21 days. The study was conducted using 4 treatments with three replications of each, namely A (control without probiotic), B (Bacillus sp. DMP13 with cell concentration of 102 CFU (colony forming unit) mL-1), C (Bacillus sp. DMP13 with 104 CFU mL-1) and D (Bacillus sp. DMP13 with 106 CFU mL-1). Probiotic was applied in the rearing media on days 9th up to 18th with 3 days intervals. The results showed that application of Bacillus sp. DMP13 with a cell density of 102 CFU mL-1 was able significantly increased the absolute larvae weight (29.67Â±1.88 mg), increased larval survival in stress concentrations of 1,250 Î¼L L-1 of formalin solution (the highest larval survival rate 98.3Â±2.9%). Treatment with 102 CFU mL-1 probiotic cell addition also increasing the total bacterial viable count in the media (the cell density reached 5.99Â±0.28 log CFU mL-1). However, other parameters such as the larvae stage index, survival rate, and absolute length of the larvae with probiotic treatments were not significantly different from those of control.

Effect of Clove Powder Syzygium aromaticum Supplementation on Growth and Health Status of Cantang Grouper (Epinephelus fuscoguttatus â™€ Ã— Epinephelus lanceolatus â™‚) in Floating Net Cage Inem Ode; Sukenda Sukenda; Widanarni Widanarni; Dinamella Wahjuningrum; Munti Yuhana; Mia Setiawati
Jurnal Perikanan Universitas Gadjah Mada Vol 25, No 1 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.78348

Cantang grouper is a hybrid grouper which is one of the main grouper species cultivated in Indonesia. This study aimed to evaluate the growth and health status of cantang grouper in floating net cages supplemented with clove powder. This study used a completely randomized design with three treatments, each with three replications. Two levels of clove powder dose used were 10 and 15 g kg-1 and one treatment without clove powder dose (control). Feed treatment using the repelletting method. Parameters observed were growth, survival rate, levels of malondialdehyde and superoxide dismutase enzyme, blood parameters, phagocity activity, total bacterial count (TBC) and total vibrio count (TVC). The results showed that the treatment of clove powder supplementation in cantang grouper feed for 30 days was able to increase the growth, survival rate, activity of the enzyme superoxide dismutase (SOD), and differential leukocyte (lymphocyte). The application of clove powder in the feed did not significantly affect the total erythrocytes, leukocytes, hematocrit, phagocytic activity, total bacterial count (TBC) and total vibrio count (TVC) of hybrid grouper in floating net cages. The conclusion of this study, administration of clove powder at a dose of 15 g kg-1 feed was able to significantly increased growth, differential leukocyte (lymphocyte), and survival rate of cantang grouper in floating net cages.

Intensive Litopenaeus vanamei pond performance with irrigation system based on Distribution of Vibrio spp. Claritha Madonsa; Bambang Widigdo; Majariana Krisanti; Munti Yuhana
Depik Vol 11, No 2 (2022): August 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Intensive shrimp ponds are characterized by the high stocking density of shrimp and artificial feeding and are equipped with an irrigation network system. The purpose of this study was to analyze the intensive L.vannamei pond with an irrigation system based on Vibrio spp. distribution to determine environmental quality degradation in terms of the treat of pathogenic bacteria. This study was carried out for one production cycle from March toJuly 2021 in an intensive shrimp farm in Sarjo Village, Pasangkayu Regency, West Sulawesi. The parameters analyzed in this study consisted of Total Bacterial Count (TBC), Total Vibrio Count (TVC), yellow and green colony Vibrio spp. in water using the spread plate method. Water samples for bacterial analysis were taken from 1) source water: (a) surface (0–50 cm depth) and (b) pump mouth; 2) treatment pond; 3) aquaculture pond; 4) Waste Water Treatment Ponds (WWTP); 5) outlet (before discharge into public waters). The results showed that the distribution of bacteria from seawater to WWTPs pond still increased the abundance of Vibrio spp., although the abundance of Vibrio spp. is still safe or below the maximum limit of 104 CFU/mL. Distribution of Vibrio bacteria in Intensive L.vannamei Pond Sarjo Village, West Sulawesi gave a relatively good pond performance because it was characterized by an abundance of Vibrio bacteria in the pond irrigation system from inlet to outlet which was still below the maximum limit and high pond productivity.Keywords:Intensive shrimp pondPathogenicVibrio spp.Pond performanceAquaculture

In Vitro Phytochemical and Inhibitory Potential Test of Bawang Hutan Bulb Extract (Eleutherine palmifolia) on Vibrio harveyi WAODE MUNAENI; ARMAN PARIAKAN; LAODE BAYTUL ABIDIN; MUNTI YUHANA
Microbiology Indonesia Vol. 11 No. 3 (2017): September 2017
Publisher : Indonesian Society for microbiology

The objectives of this study were to analyze phytochemical content of bawang hutan bulbs extract (Eleutherine palmifolia) and to test the inhibitory potential of bawang hutan bulbs extract on the growth of Vibrio harveyi bacteria at different doses. This study was conducted in March-May 2017 in Testing Laboratory of Fisheries and Marine Science Faculty of Halu Oleo University and Laboratory of Fish Health of Aquaculture Department of Fisheries and Marine Science Faculty and Laboratory of Biopharmaca of Bogor Agricultural University. Test parameter included: (1) Phytochemical test through the method of color visualization, (2) Inhibitory potential test using two methods namely agar diffusion and co-culture. Treatment of dose consisted of positive control/K+ (Chloramphenicol 30 mg/ml), negative control/K- (Sterile Aquadest) and treatment of extract included A (20 mg/ml), B (40 mg/ml), C (60 mg/ml), D (80 mg/ml). Qualitatively, result of phytochemical test showed that bawang hutan bulbs extract contained flavonoid, tannin, saponin, quinone, steroid and triterpenoid compounds. Result of inhibitory potential test indicated that treatment D obtained the highest inhibitory potential, while the minimum inhibitory potential was found in treatment A. The best co-culture test result was also found in treatment D, in which 24 hours after co-culture was performed, no V. harveyi colonies (total bacteria of 0 CFU/mL) were found. Bawang hutan bulbs extract in this study was able to inhibit the growth of V. harveyi.

Intensive Litopenaeus vanamei pond performance with irrigation system based on Distribution of Vibrio spp. Claritha Madonsa; Bambang Widigdo; Majariana Krisanti; Munti Yuhana
Depik Vol 11, No 2 (2022): August 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.2.24946

Intensive shrimp ponds are characterized by the high stocking density of shrimp and artificial feeding and are equipped with an irrigation network system. The purpose of this study was to analyze the intensive L.vannamei pond with an irrigation system based on Vibrio spp. distribution to determine environmental quality degradation in terms of the treat of pathogenic bacteria. This study was carried out for one production cycle from March toJuly 2021 in an intensive shrimp farm in Sarjo Village, Pasangkayu Regency, West Sulawesi. The parameters analyzed in this study consisted of Total Bacterial Count (TBC), Total Vibrio Count (TVC), yellow and green colony Vibrio spp. in water using the spread plate method. Water samples for bacterial analysis were taken from 1) source water: (a) surface (0–50 cm depth) and (b) pump mouth; 2) treatment pond; 3) aquaculture pond; 4) Waste Water Treatment Ponds (WWTP); 5) outlet (before discharge into public waters). The results showed that the distribution of bacteria from seawater to WWTPs pond still increased the abundance of Vibrio spp., although the abundance of Vibrio spp. is still safe or below the maximum limit of 104 CFU/mL. Distribution of Vibrio bacteria in Intensive L.vannamei Pond Sarjo Village, West Sulawesi gave a relatively good pond performance because it was characterized by an abundance of Vibrio bacteria in the pond irrigation system from inlet to outlet which was still below the maximum limit and high pond productivity.Keywords:Intensive shrimp pondPathogenicVibrio spp.Pond performanceAquaculture

Intensive Litopenaeus vanamei pond performance with irrigation system based on Distribution of Vibrio spp. Claritha Madonsa; Bambang Widigdo; Majariana Krisanti; Munti Yuhana
Depik Vol 11, No 2 (2022): August 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.2.24946

Intensive shrimp ponds are characterized by the high stocking density of shrimp and artificial feeding and are equipped with an irrigation network system. The purpose of this study was to analyze the intensive L.vannamei pond with an irrigation system based on Vibrio spp. distribution to determine environmental quality degradation in terms of the treat of pathogenic bacteria. This study was carried out for one production cycle from March toJuly 2021 in an intensive shrimp farm in Sarjo Village, Pasangkayu Regency, West Sulawesi. The parameters analyzed in this study consisted of Total Bacterial Count (TBC), Total Vibrio Count (TVC), yellow and green colony Vibrio spp. in water using the spread plate method. Water samples for bacterial analysis were taken from 1) source water: (a) surface (0–50 cm depth) and (b) pump mouth; 2) treatment pond; 3) aquaculture pond; 4) Waste Water Treatment Ponds (WWTP); 5) outlet (before discharge into public waters). The results showed that the distribution of bacteria from seawater to WWTPs pond still increased the abundance of Vibrio spp., although the abundance of Vibrio spp. is still safe or below the maximum limit of 104 CFU/mL. Distribution of Vibrio bacteria in Intensive L.vannamei Pond Sarjo Village, West Sulawesi gave a relatively good pond performance because it was characterized by an abundance of Vibrio bacteria in the pond irrigation system from inlet to outlet which was still below the maximum limit and high pond productivity.Keywords:Intensive shrimp pondPathogenicVibrio spp.Pond performanceAquaculture

Diversity and the Composition of Fatty Acids of Lipolytic Bacteria Isolated from Soil and Aquatic Sediment in a Forest and on an Oil Palm Plantation Wijayanti, Marini; Meryandini, Anja; Wahyudi, Aris Tri; Yuhana, Munti
Makara Journal of Science Vol. 18, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

The specific bacteria in forests and on oil palm plantations are lipolytic bacteria. Their enzymes have been applied in the agro-aquaculture, food, detergent, pharmaceutical, dairy, and biodiesel-biokerosene industries. This study describes the diversity of cultivable lipolytic bacteria from soil and aquatic sediment in a forest and on an oil palm plantation and their fatty acid products. Soil samples used in this research were obtained from topsoil in a lowland forest and on an oil palm plantation and from sediments in fresh water near these sites. The forest is located in Bukit Duabelas National Park, and the oil palm plantation is near the forest in Sarolangun District, Jambi Province, Indonesia. Twenty-two isolates of lypolitic bacteria were selected from 32 isolates grown in lipolytic selective medium. The 22 consisted of 11 isolates from topsoil and 11 from aquatic sediment from the forest and plantation area. These isolates were identified by 16S rRNA-sequence data analysis. Taxonomically, they belonged to five genera: Burkholderia, Cupriavidus, Serratia, Acinetobacter, and Kurthia. The maximum likelihood tree showed that they are phylogenetically distributed in three clusters. They were clustered into three groups: the Burkholderia-Cupriavidus group, the Serratia-Acinetobacter group, and the Kurthia group. Their lipolytic enzymes formed various fatty acids after analysis by gas chromatography-flame ionization detector (GC-FID). Some isolates formed essential fatty acids, such as linoleic, linolenic, arachidonic, eicosapentanoic acid (EPA), and docosahexanoic acid (DHA).

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