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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Teknologi Perikanan dan Kelautan Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Akuakultur Rawa Indonesia ILMU KELAUTAN: Indonesian Journal of Marine Sciences Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Omni-Akuatika Proceeding Simposium Nasional Kelautan dan Perikanan Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Media Akuatika: Jurnal Ilmiah Jurusan Budidaya Perairan Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Makara Journal of Science Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine
MUNTI YUHANA
Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan - IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Library & Information Science Medicine & Pharmacology Social Sciences Veterinary

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BACTERIAL COMMUNITY SHIFTS OF A HIGH MOUNTAIN LAKE IN RESPONSE TO VARIABLE SIMULATED CONDITIONS: AVAILABILITY OF NUTRIENTS, LIGHT AND OXYGEN YUHANA, MUNTI; HORATH, THOMAS; HANSELMANN, KURT
BIOTROPIA Vol. 13 No. 2 (2006): BIOTROPIA Vol. 13 No. 2 December 2006
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (586.95 KB) | DOI: 10.11598/btb.2006.13.2.219

Abstract

We studied bacterial population composition shifts by exposing natural water samples to variable simulated environmental conditions. The samples were taken from Lake Jori XIII (2640 m a.s.l), an oligo-to mesotrophic cold freshwater lake, located in the eastern Swiss Alps. The Jori lakes are characterized as remote, unpolluted high mountain lakes with a long period of ice cover and typically low nutrient concentrations. Culture independent techniques (PCR-based analyses) were used for detection and molecular characterization of a large number of bacteria most of which are still uncultivable. Bacterial community shifts over three ecological conditions (nutrients, light and oxygen availability) were detected by using Temporal Temperature gradient Gel Electrophoresis (TTGE) of a PCR-amplified part of the 16S rRNA gene. The bacterial populations responded differently to the variable conditions, as revealed by TTGE pattern shifts during the experiment. Key words: Temporal Temperature gradient Gel Electrophoresis (TTGE), ARB, small subunit ribosomal RNA gene (SSU rRNA gene), alpine freshwater lake JOri XIII, PCR
EFFECTS OF HUMIC ACID ADDITION TO FEEDS WITH HEAVY METAL CADMIUM CONTAMINATION FROM GREEN MUSSELS ON THE GROWTH PERFORMANCE OF ASIAN SEABASS Rasidi, Rasidi; Jusadi, Dedi; Setiawati, Mia; Yuhana, Munti; Jr., Muhammad Zairin; Sugama, Ketut
BIOTROPIA Vol. 26 No. 3 (2019): BIOTROPIA Vol. 26 No. 3 December 2019
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.409 KB) | DOI: 10.11598/btb.2019.26.3.1114

Abstract

Although it contains heavy metals, the highly nutritious green mussel Perna viridis is used as a fish meal replacement in fish diets. Fortunately, humic acid (HA) has the ability to chelate heavy metals in animal feeds. Its addition to fish feed formulation is, therefore, needed to prevent the accumulation of heavy metals in the fish's body. Hence, an experiment using a Completely Randomized Design (CRD) with 5 treatments and 3 replications was conducted to evaluate the performance and feed efficiency of Asian seabass Lates calcarifer juveniles with the addition of HA to their green mussel–based diet containing the heavy metal cadmium (Cd). Five experimental diets were formulated with different dosages of HA addition at 0, 400, 800, 1200, and 1600 mg kg⁻¹ of feed as treatments A, B, C, D, and E, respectively. The experimental diets were given to triplicate groups of 15 Asian seabass juveniles with an initial body weight of 4.30 ± 0.60 g over a 70-day cultivation period, feeding until satiation. The results showed that feed consumption, feed digestibility, protein retention, growth performance, and feed efficiency were significantly affected by HA addition in the fish diet. Among all treatments, HA addition of 1600 mg kg⁻¹ produced the best biological response from the Asian seabass. To conclude, the HA addition of 1600 mg kg⁻¹ into the diet was the best dosage, resulting in optimal growth performance and feed efficiency in Asian seabass. The highest dose of HA in Cd-contaminated feeds could reduce Cd content, but had not yet been able to eliminate Cd in fish meat. Therefore, green mussel meal, with HA addition as an alternative protein source, has potential as a feed additive for Cd-contaminated diets of Asian seabass juveniles.
EFFECT OF NEGATIVE REDOX POTENTIAL OF DRY SEDIMENT ON THE INFECTIVITY OF Vibrio parahaemolyticus, GROWTH AND HEALTH STATUS OF PACIFIC WHITELEG SHRIMP Putri, Shofii Amaliah; Sukenda, Sukenda; Yuhana, Munti; Nirmala, Kukuh
Indonesian Aquaculture Journal Vol 20, No 2 (2025): (December, 2025)
Publisher : Agency for Marine and Fisheries Extension and Human Resources

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.20.2.2025.133-144

Abstract

Sediment serves as a site for the accumulation of aquaculture waste. Oxidation-Reduction Potential (ORP) is an index indicating anaerobic conditions. A combination of host, pathogen, and suboptimal environmental quality can lead to disease outbreaks. One such disease is Acute Hepatopancreatic Necrosis Disease (AHPND), caused by Vibrio parahaemolyticus. This study was aimed to analyse the impact of sediment drying on infectivity of V. parahaemolyticus growth and health status of Pacific whiteleg shrimp. The sediment ORP used at the initial study was -37 mV. The sediment was dried for 120 hours at a temperature of 40.75 ± 2.73 °C. A total of 15 shrimp/aquarium with an average body weight of 2.35±0.22 g/individual were reared in aquarium sized 20x25x30 cm3 containing dried and undried sediment for 120 hours, then contaminated with 105 CFU/mL V. parahaemolyticus through immersion from the start of rearing until 20 days later. The results showed that sediment drying was able to reduce bacterial abundance and increase ORP sediment. The longer duration of cultivation, the more the sediment ORP decreased. Sediment ORP decreased with increasing shrimp farming duration. The highest bacterial abundance in the sediment on thrid phase of study was observed in the positive control treatment on D+10 ((2.02±0.39)x104 CFU/g), then it decreased until the end. Meanwhile, bacterial abundance in water and hepatopancreas decreased until the end of the study. Survival rates, immune responses, and growth parameters of shrimp with sediment drying were higher than the positive control (p<0.05) but not significantly different from the negative control. The histopathology of shrimp hepatopancreas profile in the sediment drying treatment was better compared to the positive control.
EFFICACY OF DIETARY SUPPLEMENTATION OF AMBON BANANA MIDRIB SIMPLICIA TO ENHANCE GROWTH PERFORMANCE, HEMATOLOGICAL PROFILE, IMMUNITY, AND SURVIVAL OF CATFISH CHALLENGED WITH Edwardsiella tarda Wahjuningrum, Dinamella; Nugroho, Riyanto; Yuhana, Munti; Citarasu, Thavasimuthu; Abdullah, Taufiq
Jurnal Riset Akuakultur Vol 20, No 3 (2025): September (2025)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.20.3.2025.229-244

Abstract

Catfish (Clarias sp.) is a significant aquaculture species, but its production is often limited by Edwardsiella tarda infections, which cause substantial losses. Plant-derived alternatives such as banana midrib simplicia offer promising solutions to enhance fish health and reduce antibiotic dependence. This study investigated the effects of Ambon banana (Musa paradisiaca var. sapientum) midrib simplicia on growth performance, hematological parameters, immune responses, and resistance to E. tarda infection in catfish. A completely randomized design was used with five treatments and three replications, consisting of a positive control, a negative control, and commercial feed supplemented with 2% (B2), 3% (B3), and 4% (B4) banana midrib simplicia. The feeding trial lasted 30 days, after which fish were intramuscularly injected with E. tarda (10⁷ CFU mL⁻¹). Growth performance, hematological indicators (red blood cell count, hemoglobin concentration, hematocrit, and white blood cell count), immune responses (phagocytic and respiratory burst activities), and survival rate were evaluated. Dietary supplementation with banana midrib simplicia significantly improved growth performance, hematological parameters, immune responses, and survival in catfish challenged with E. tarda compared to the control groups. The optimal supplementation dose was 3%, providing the most significant improvement in growth, immunity, and survival. These findings highlight the potential of banana midrib as a functional feed additive derived from agricultural by-products to promote fish health and aquaculture productivity. Ikan lele (Clarias sp.) merupakan salah satu komoditas penting dalam akuakultur, namun produksinya sering terkendala oleh infeksi Edwardsiella tarda yang menyebabkan kerugian signifikan. Alternatif berbasis tanaman seperti simplisia pelepah pisang menawarkan solusi potensial untuk meningkatkan kesehatan ikan dan mengurangi ketergantungan terhadap antibiotik. Penelitian ini mengevaluasi pengaruh simplisia pelepah pisang Ambon (Musa paradisiaca var. sapientum) terhadap kinerja pertumbuhan, parameter hematologis, respons imun, dan ketahanan terhadap infeksi E. tarda pada ikan lele. Penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan dan tiga ulangan, terdiri atas kontrol positif, kontrol negatif, serta pakan komersial yang disuplementasi simplisia pelepah pisang pada dosis 2% (B2), 3% (B3), dan 4% (B4). Uji pemberian pakan dilakukan selama 30 hari, kemudian ikan diinjeksi intramuskular dengan E. tarda (10⁷ CFU mL⁻¹). Parameter yang diamati meliputi pertumbuhan, indikator hematologis (jumlah eritrosit, konsentrasi hemoglobin, hematokrit, dan jumlah leukosit), respons imun (aktivitas fagositosis dan respiratory burst), serta tingkat kelangsungan hidup. Suplementasi pakan dengan simplisia pelepah pisang secara signifikan meningkatkan performa pertumbuhan, parameter hematologis, respons imun, dan kelangsungan hidup ikan lele yang diinfeksi E. tarda dibanding kelompok kontrol. Dosis optimal diperoleh pada suplementasi 3%, yang memberikan peningkatan terbesar pada pertumbuhan, imunitas, dan kelangsungan hidup. Temuan ini menunjukkan bahwa simplisia pelepah pisang memiliki potensi sebagai aditif pakan fungsional berbasis limbah pertanian untuk meningkatkan kesehatan ikan dan produktivitas akuakultur.
RESPONS IMUN UDANG VANAME PADA SISTEM BIOFLOK DENGAN KEPADATAN BERBEDA TERHADAP INFEKSI Vibrio parahaemolyticus Widanarni, Widanarni; Fadilah, Iin Nur; Cahyawati, Nadia; Yuhana, Munti; Sukenda, Sukenda; Gustilatov, Muhamad
Media Akuakultur Vol 20, No 2 (2025): Desember 2025
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.20.2.2025.51-61

Abstract

Budidaya udang vaname (Litopenaeus vannamei) pada kepadatan tinggi meningkatkan stres dan risiko infeksi bakteri, khususnya Vibrio parahaemolyticus. Teknologi bioflok (BFT) melalui penambahan karbon  organik dapat meningkatkan biomassa mikroba heterotrof yang memperbaiki respons imun dan menurunkan virulensi bakteri. Penelitian ini bertujuan menganalisis respons imun dan resistansi udang yang dipelihara dengan padat tebar berbeda dalam sistem bioflok terhadap infeksi V. parahaemolyticus. Udang (0,44±0,02 g) dipelihara selama 7 hari sebanyak 16 perlakuan hasil kombinasi sistem bioflok dan kontrol (tanpa bioflok) dengan kepadatan 4, 8, 12, dan 16 ekor L-¹, lalu diuji tantang melalui perendaman dengan dosis V. parahaemolyticus 10u CFU mL-¹ dimulai sejak awal perlakuan. Hasil menunjukkan bahwa sistem bioflok meningkatkan parameter imun udang, termasuk total hemocyte count (THC), aktivitas fagositik (AF), respiratory burst (RB), dan aktivitas phenoloxidase (PO) secara signifikan (P<0,05) dibandingkan kontrol. Kelimpahan V. parahaemolyticus di air dan tubuh udang perlakuan bioflok lebih rendah dan berbeda signifikan (P<0,05) dengan kontrol positif. Bioflok juga meningkatkan tingkat kelangsungan hidup, dengan nilai pada kepadatan 4 ekor L-¹ (83,33±5,77%), dan tetap lebih baik dibandingkan kontrol hingga kepadatan 16 ekor L-¹. Sistem bioflok terbukti efektif meningkatkan respons imun, menekan infeksi V. parahaemolyticus, dan mempertahankan kelangsungan hidup udang vaname pada kepadatan tinggi.
Co-Authors , Alimuddin , Rahman, , Achmad Farouq Ade Dwi Sasanti Adna Sumadikarta ADNI OKTAVIANA Afif, Usamah Afiff , Usamah Agus Alim Hakim Aldy Mulyadin Aliati Iswantari Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Angela M Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti ANGELA MARIANA LUSIASTUTI Angela Mariana Lusiastuti Anis Zubaidah Anja Meryandini Annisa Astri Anggraeni Arief Muhammad Arini Resti Fauzi Aris Tri Wahyudi Ayi Santika Ayi Santika Bako, Surandha Cahyawati, Nadia Citarasu, Thavasimuthu Claritha Madonsa Claritha Madonsa Darna Andrian Ramadhan DEDI JUSADI Destianingrum, Nurin Dalilah Ayu Dinamella Wahjuningrum Eddy Supriyono Efianda, Teuku Reza Emei Widiyastuti Enang Harris Enang Harris Enang Harris Endang Susianingsih Endang Susianingsih Endang Susianingsih Engelhaupt, Martin Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fadilah, Iin Nur Faoziyatunnisa, Nurul Fauzi, Arini Resti Filibertus Tantio Firdausi, Amalia Putri Fitriana Nazar Gustilatov, Muhamad Hamida Pattah Hamsah Hamsah Hary Krettiawan Hasan Nasrullah Hasan Nasrullah Hermawaty Abubakar Hessy Novita Hessy Novita HORATH, THOMAS Iis Sumartini Iman Rusmana Ince Ayu Khairana Kadriah Ince Ayu Khairana Kadriah Ince Ayu Khairani Kadriah Inem Ode Inka Destiana Sapitri Irzal Effendi Istiqomah, Amalia Jr., Muhammad Zairin Julie Ekasari Ketut Sugama Ketut Sugama Khasanah, Noviati Rohmatul Kukuh Nirmala KURT HANSELMANN Kustiariyah Tarman La Ode Baytul Abidin La Ode Muhammad Arsal Laely Nuzullia Lili Sholichah Lilik Setiyaningsih Linuwih Aluh Prastiti M. Zairin Junior Majariana Krisanti Marini Wijayanti Mia Setiawati Mira Mawardi Muhammad Arif Mulya Muhammad Zairin Jr. Muharijadi Atmomarsono Nasrullah, Hasan Neltje Nobertine Palinggi Nugroho, Riyanto Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nuri Kamaliah, Syarifah Nuzullia, Laely Ode, Inem Pariakan, Arman Putra, The Best Akbar Esa Putri Shandra Ramhirez Putri, Shofii Amaliah Ramhirez, Putri Shandra Ranta Sumadi Rasidi Rasidi Rasidi, Rasidi Ricko Reynalta Rini, Adityawati Fajar Ronald Kriston Sauttua Nainggolan Rr. Bellya Anasti Maharani S.H. Dwinanti Salamah Salamah Sefti Heza Dwinanti Soko Nuswantoro Sri Hariati Sri Nuryati Sudrajat, R Herman Sugeng Santoso Sukenda . Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Suleman, Gabriella Augustine Syamsiar, Syamsiar Tambun, Andreas Taufiq Abdullah, Taufiq Tri Heru Prihadi Tsani Untsa, Agista Usman Usman Usman Usman UTUT WIDYASTUTI WAODE MUNAENI WAWAN ABDULLAH SETIAWAN Wicaksono, Baref Agung WIDANARNI WIDANARNI Woro Nur Endang Sariati Yanti Inneke Nababan Yunarty Yunarty Yusli Wardiatno Zakki Zainun Zulfani, Anisa