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Biofilm Effectivity of Ethanol Extracts (Casuarina equisetifolia) Leaves of the Bacteria Enterococcus faecalis Debby Rosalina; Aprilia
Denta Journal Kedokteran Gigi Vol 14 No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v14i2.1

Abstract

Background: Enterococcus faecalis is one of the bacteria that can form biofilms that can cause persistent endodontic infections. Biofilm is the unity of microbial cell surface surrounded by a matrix of extracellular polymeric substances (EPS). The ethanol extracts of Casuarina equisetifolia leaves are known to have potentially bioactive compounds as antibacterials such as flavonoids, saponins, tannins, phenols, and alkaloids. Objective: To determine the power antibiofilm ethanol extracts of Casuarina equisetifolia leaves at multiple concentrations of the bacteria Enterococcus faecalis. Methods: This study is an experimental research design post-test only control group design. The sample divided into 5 groups consisted of positive control Enterococcus faecalis in media Trypticase Soy Broth (TSB)+DMSO 1%, and 4 treatment groups of ethanol extracts of Casuarina equisetifolia leaves a concentration of 0,5%, 1%, 1,5%, and 2%. Biofilm is made using Enterococcus faecalis ATCC 29212 bacteria were cultured on TSB media were incubated for 1x24 hours. 0,1 ml of the bacteria Enterococcus faecalis with a concentration of 106 put on a plate micro titter then done using crystal violet staining. Biofilm-checked by measuring optical density (OD) values ​​using the ELISA reader. Data analysis used Kruskal-Wallis followed by Mann-Whitney test. Results: The ethanol extracts Casuarina equisetifolia leaves on the growth of Enterococcus faecalis bacteria indicate the presence of power antibiofilm. The results mean % mortality of bacteria (p<0.05) were significant in all groups that have the power antibiofilm effectiveness as against the growth of bacteria Enterococcus faecalis. Conclusion: The ethanol extracts Casuarina equisetifolia leave have antibiofilm power against bacterial growth Enterococcus faecalis.
Invitro Micro Encapsulation of Beta Tri Calcium Phosphate from Anadara granosa Shell Synthesis Diana Soesilo; Aprilia; Moh. Basroni Rizal
Denta Journal Kedokteran Gigi Vol 14 No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v14i2.3

Abstract

Background: Calcium is a material that is mostly contained in the Anadara-granose shell. Beta-TCP can be obtained from the hydrothermal process from the Anadara-granose shell. Beta-TCP has a chemical composition that approximates the structure of bones and teeth. Objective:The microencapsulation technique aims to increase stability, reduce side effects and toxic effects of drugs, and prolong the release of ingredients. The encapsulation process is an attempt to inhibit the dissolution speed of Calcium to prevent tunnel defects. Methods: Anadara-granose shell powder was subjected to hydrothermal processing for 18 hours and sintering for 3 hours. The beta-TCP powder was dissolved with aquadest using a magnetic stirrer until it was homogeneous, Na-alginate was dissolved in aquadest until it was homogeneous with a magnetic stirrer then the two solutions were mixed and the CaCl2 solution was dropped. The sample was divided into 3 groups; Pure Beta-TCP(K-); 7 hours stirring (P1); 8 hours stirring (P2). After completion of the stirrer, the samples were centrifuged at 2500 rpm for 6 minutes, then freeze-dried for 12 hours. The level test was carried out using complexometry comparing the pure Beta-TCP group with the Beta-TCP stirrer encapsulation process for 7 hours and 8 hours. Results: The data showed that the average calcium level in K(-) group with pure Beta-TCP was 8.63%, the P1 Beta-TCP group with 7 hours stirrer 2.86%, and the P2 Beta-TCP group with 8 hours stirrer 2.12%. Conclusion: In the Anadara-granosa shell nanoencapsulation process, the calcium level gradually decreased with the longer duration of stirring time
Bioviability of Mangrove Leaves Extract (Sonneratia alba) on Fibroblast BHK-21 Cell Culture Arlita Gladys Tricia Charyadie; Aprilia; Widyastuti
Denta Journal Kedokteran Gigi Vol 11 No 2 (2017): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: Dental caries is a major oral health problem which is most commonly found in Indonesia. One of the main factors in role of causing dental caries is Streptococcus mutans which is the primary target of the prevention of caries. Leaf extract of Sonneratia alba has been proven to have an antimicrobial effect towards Streptococcus mutans, but its cytotoxic activity on normal cells is still unknown. The initial cell viability in this study was conducted using cell line BHK-21. Purpose: The aim of this study is to determine the cell viability of fibroblast cells line BHK-21 by given Sonneratia alba’s leaf extract. Materials and Methods: The experiment was conducted using post test only control group design. BHK-21 fibroblast cell culture on 96 wells were divided into cell control group (n=9), media control group (n=9) and test groups (n=9). The test groups were given various concentration of Sonneratia alba’s leaf extract, they were: 5%; 10%; and 20%. The cell cultures were incubated for 24 hours before and after treatment using Sonneratia alba’s leaf extract. After dripping MTT into the microplates, OD were read using ELISA reader and the viability were counted. The data that was acquired from the viability calculation was statistically analyzed using One Way ANOVA and LSD test. Results: Data showed the increase of viability cell in all the test groups. The highest viability cell showed by the test group with 10% concentration of Sonneratia alba (153,7122%±7,42458), and the lowest found in the test group with 20% concentration of leaf extract (141,4682%±7,48752). Conclusion: In this experiment, the leaf extract of Sonneratia alba proved to be nontoxic on fibroblast cells line BHK-21, the extract increased the viability and also caused proliferation of fibroblast cells.
Marketing Strategy Based on Image of Hang Tuah University Dental and Oral Hospital in 2015 Annisa Mutiara Pertiwi; Dwi Hariyanto; Aprilia
Denta Journal Kedokteran Gigi Vol 11 No 1 (2017): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: Individuals in our contemporary society tend to depend on various types of services, including health care service. Managers of health care services must be aware of this situation to improve image and reputation as a public’s choice services. Image is an award obtained by a company for their excellence in services. In improving the image, a company needs the public’s or consumer’s appraisals for a marketing strategy formation. Purpose: As information to develop the image of Dental and Oral Hospital of Hang Tuah University, Surabaya. Materials and Methods: descriptive analysis with cross sectional research design was used in this study. Simple random sampling of probability sampling technique was used to choose 100 respondents of this study. Respondents were given a questionnaire to assess the image of Dental and Oral Hospital, Hang Tuah University Surabaya. The results of the questionnaire were analyzed by using descriptive statistics. Result: Results on the image of Dental and Oral Hospital of Hang Tuah University showed that 83 respondents rated moderately good image, while 17 respondents rated poor image. Conclusion: Based on the SWOT matrix, it can be concluded that several development through consideration of internal and external factors can be beneficial to the progress of Dental and Oral Hospital of Hang Tuah University, Surabaya.
Efektivitas Antibakteri Ekstrak Daun Mangrove Acanthus ilicifolius Terhadap Biofilm Enterococcus faecalis Hariningtyas Dian Rachmawati; Aprilia; Kristanti Parisihni
Denta Journal Kedokteran Gigi Vol 9 No 2 (2015): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Abstract

Background: Enterococcus faecalis is one of the bacteria which have ability to form biofilms that can cause persistent endodontic infections. Biofilm is a communities of well-organized bacteria adhere to the surface and coated by layer of extracellular polysaccharide matrix. The extracts of mangrove Acanthus ilicifolius leaves have been known to have potentially bioactive compounds as antibacterial such as saponins, alkaloids, terpenoids, and tannins. Purpose: The aim of this study was to determine the antibacterial effectiveness of mangrove Acanthus ilicifolius leaves extracts to Enterococcus faecalis biofilm. Materials and Methods: This study was true experimental laboratory with post test only control group design. The sample in this study was Enterococcus faecalis, divided into 6 groups consisted of control positive (NaOCl 2.5%) group, and 5 treatment groups of mangrove Acanthus ilicifolius leaves extract with different concentration of 60 mg/ml; 70 mg/ml; 80 mg/ml; 90 mg/ml; and 100mg/ml and be repeated 8 times. Enterococcus faecalis bacteria were cultured in mediaTSBglu incubated for 24 hours. Amount of 0.1 ml of bacteria Enterococcus faecalis with concentrations of 106were loaded on microtiter plate then stained with crystal violet. Biofilms checked by measuring optical density (OD) using an ELISA reader. Data were analyzed by One-way ANOVA followed by Post Hoc test. Result: Acanthus ilicifolius leaves extract decrease the OD of Enterococcus faecalis biofilm (p<0,05) in all groups. Conclusion: The extract of mangrove Acanthus ilicifolius leaves extract have antibacterial efficacy against Enterococcus faecalis biofilm.
Pengaruh Metode PQRST dalam Pembelajaran Keterampilan Membaca Teks Deskripsi Siswa Kelas VII SMP Negeri 3 Kota Sungai Penuh Aprilia, Aprilia; Afnita, Afnita
MASALIQ Vol 3 No 4 (2023): JULI
Publisher : Lembaga Yasin AlSys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/masaliq.v3i4.1175

Abstract

This study aims to see the effect of the PQRST method in learning the skills of reading descriptive texts for class VII students of SMP Negeri 3 Kota Sungai Lilin. The type of research used in this research is quantitative research with a quasi-experimental method using a one group pretest-posttest design. The population in this study were 105 class VII students of SMP Negeri 3 Kota Sungai Kunci for the academic year 2022/2023, with a sample size of 26 students who were determined using a purposive sampling technique. Based on the results of the posttest and hypothesis testing, it was found that the average value of skills in reading descriptive texts after using the PQRST method was higher than the average value of skills in reading descriptive texts before using the PQRST method. learning the skills of reading descriptive text after using the PQRST method is in the Very Good (BS) classification with an average value of 96.15. When compared with the learning value of reading skill description text for class VII students of SMP Negeri 3 Kota Sungai Lilin before using the PQRST method it is lower and is in the Less Enough (KC) classification with an average value of 15.38. Based on the t-test it was concluded that the hypothesis (H1) was accepted at a significant level of 95% and dk = (n-1) because tcount > ttable (20.26>1.70). Thus, it can be concluded that there is an effect of the use of the PQRST method on the skills of reading descriptive texts for class VII students of SMP Negeri 3 Kota Sungai Lilin.
Rancangan Teknis Penambangan dan Penjadwalan Produksi Jangka Pendek (Short Term Scheduling) pada Penambangan Bijih Nikel PT. Gerbang Multi Sejahtera Aprilia, Aprilia; Rembah, Rina; Nurfasiha, Nurfasiha
Mining Science And Technology Journal Vol 3 No 1 (2024): Mining Science and Technology Journal
Publisher : Program Studi Teknik Pertambangan, Fakultas Teknik, Universitas Sulawesi Tenggara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54297/minetech-journal.v3i1.550

Abstract

Block U8 is one of the front of PT. Gerbang Multi Sejahtera which is still virgin condition with an area 14,5 ha. The aim of this research is to estimate reserves, design mining techniques, and make production cheduling based on the design result. The data needed in this research is drill logs. Data from the result of estimating laterite nikel reserve using the Kriging method using Surpac 6.5.1 software shows that the one tonnage is 1.063.919 tons with an average Ni content of 1,80%. The tier geometry used is in accordance with the company’s geotechnical records, namely a tier height of 5 m, a tier depth of 2 m and a single tier slope of 50o. The width of the haul road on a straight road is 12,63 m dan the width of the haul road on bends is 13,97 m, the road grade used is 12%. The planned mining method is open pit which is divided into 3 sequences and consists of 21 ming pushback with a minr life 21 month. The need for Excavator Doosan DX 200A must-dig tools for the progress of the mine from the 1st moth to the 21st month are constant. 1 unit OB digging tool, 1 unit of Ore digging tool, and 1 unit of mush material Ore. The mean of transportation killed were 3 unit in Pit 1 and Pit 2, while in Pit 3 it was 4 units.
Pendidikan Dan Pelatihan Kader Sebagai Salah Satu Pilar Penting Perubahan Perilaku Di Bidang Kesehatan Gigi Dan Mulut Pranoto, Amelia Elizabeth; Aprilia, Aprilia; Rahmitasari, Fitria; Soesilo, Diana; Puspita, Sinta; Riski, Mardiyanti; Ariestania, Vivin; Lestari, IGAM Oka; Raharjo, Pambudi; Wijaya, Yongki Hadinata; Rizal, Mohammad Basroni; Sarianoferni, Sarianoferni; Hendro, Onge Victoria; Wira, Rizko; Sularsih, Sularsih; Cahyo, Benny Dwi; Paramita, Putri
Jurnal Pengabdian Masyarakat Bangsa Vol. 2 No. 11 (2025): Januari
Publisher : Amirul Bangun Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59837/jpmba.v2i11.1930

Abstract

Lima dari sepuluh anak-anak Indonesia usia sekolah dasar memiliki masalah gigi berlubang. Hal ini disebabkan oleh kebersihan rongga mulut yang buruk dan kurangnya pengetahuan anak-anak terkait pentingnya menjaga kesehatan gigi dan mulut. Anak-anak juga seringkali tidak menyikat gigi di waktu yang benar, ditambah lagi cara menyikat gigi yang keliru menjadi salah satu faktor munculnya gigi berlubang. Dokter gigi dari Fakultas Kedokteran Gigi, Universitas Hang Tuah Surabaya melakukan kegiatan pengabdian masyarakat berupa School Health Program – Training of Trainer (SHP-ToT). Kegiatan ini bertujuan untuk menciptakan kader kesehatan gigi dan mulut yang berasal dari lingkungan internal sekolah. Diharapkan kader-kader tersebut dapat menjadi pilar penggerak utama pada pendidikan anak-anak usia sekolah dasar akan pentingnya menjaga kebersihan gigi dan mulut secara berkelanjutan. Kader akan diberi pelatihan dan diuji sejauh mana pemahamannya terkait kesehatan gigi dan mulut. Setelah pelatihan selesai, kader akan terus dipandu untuk mengajarkan anak-anak sekolah dasar tentang pentingnya menjaga kesehatan rongga mulut sekaligus menjadi supervisor anak-anak agar mau menyikat gigi pagi dan malam selama 21 hari berturut-turut.
Pengembangan Produksi Produk Usaha Mikro, Kecil, dan Menengah Kerupuk Sagu Berbasis Elektronik Devi Wedayanti, Made; Siswanto, Apri; Sundari, Eva; Kusumawaty, Yeni; Faiser, Adli; Aprilia, Aprilia
ABDINE: Jurnal Pengabdian Masyarakat Vol. 4 No. 2 (2024): ABDINE : Jurnal Pengabdian Masyarakat
Publisher : Sekolah Tinggi Teknologi Dumai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52072/abdine.v4i2.976

Abstract

Pengabdian Masyarakat ini merupakan hasil kompetisi Hibah Dikti  Skema Pemberdayaan Berbasis Kewirausahaan Ruang Lingkup Pemberdayaan Mitra Usaha Produk Unggulan Daerah tahun 2024. Alasan Pemilihan topik  atau pentingnya topik pengabdian ini karena kerupuk Sagu merupakan produk unggulan Kabupaten Kuantan Singingi Provinsi Riau yang sudah terkenal dan sudah ada sejak tahun 1983. Sehingga sangat perlu dikembangkan dan dilestarikan.   UMKM Kerupuk Sagu Uwit (mitra 1) dan Kerupuk Sagu Sina (mitra 2) menjadi mitra pada pengabdian masyarakat ini. Permasalahan PKM ini adalah  kurangnya sumber daya produksi yang memadai, seperti bahan baku, mesin, peralatan, atau tempat usaha. Proses produksi masih mengaduk adonan sagu dengan tangan dan masih memotong kerupuk sagu dengan potongan tradisional sehingga membuat keterbatasan produksi karena memakan waktu lebih lama.  Metode PKM ini adalah  melakukan tahapan Sosialisasi- Pemberian bantuan mesin mesin produksi- pelatihan- penerapan teknologi- keberlanjutan program. Ringkasan Hasil PKM ini berjalan dengan sukses dengan antusias mitra yang tinggi , ada Peningkatan Kualitas Produk dengan meningkatnya jumlah produksi, mitra 1 dari 400 Kg /bulan menjadi 740 Kg/ bulan. Mitra 2 dari 320 Kg/bulan menjadi 552 Kg/ bulan,  dan adanya peningkatan pengetahuan mitra  menggunakan mesin produksi sebelum PKM dan sesudah PKM.
INDEKS DMF-T dan def-t MURID USIA 7-10 TAHUN MI TARBIYATUT THOLABAH LAMONGAN Kurniawan, Hansen; Fitriani, Yufita; Sari, Rima Parwati; Handayani, Budi; Rahmitasari, Fitria; Prabowo, Puguh Bayu; Aprilia, Aprilia; Ariestania, Vivin; Priambodo, Nur Tsurayya; Saptaswari, Dianty
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 1 (2025): MAJU : Indonesian Journal of Community Empowerment, Januari 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/fwwcfg92

Abstract

Background Based on the 2018 Basic Health Research (Riskesdas), it was reported that the prevalence of dental and oral problems in Indonesia is still very high, namely 57.6 percent. Of this figure, only 10.20% have received treatment. Dental and oral health in Indonesia, especially in coastal areas, is still a complex problem. (Komarudin, Apriasari and Azizah, 2021) Lack of public attention and equal distribution of health facilities, especially in the field of Dentistry, are still factors that influence public understanding of dental and oral health. Dental and oral health status can be assessed using the DMF-T and def-t indices. Objective To assess the dental and oral health status in children aged 7-10 years MI Tarbiyatut Tholabah, Paciran Lamongan Method Dental and oral examination of children aged 7-10 years MI by assessing the DMF-T and def-t indices. Conclusion The results of the dental and oral examination of students at MI Tarbiyatut Tholabah, Paciran Lamongan with a def-t value of 2.71 which means the dental and oral health status with moderate results, and the DMF-T index with a result of 0.56 which means very low.
Co-Authors Abidin, Muhammad Ayassy Malik Afnita Afnita Agung Prihantoro Alfarisi, Muhammad Farhan Anastasya, Diana Ayu ANDIKA SAPUTRA Annisa Mutiara Pertiwi Apri Siswanto Apriyanti, Rina Ardiansyah Kurniawan Ariestania, Vivin Arlita Gladys Tricia Charyadie Bayu Rachman Budiyono Budiyono Cahyo, Benny Dwi Charyadie, Arlita Gladys Tricia Dakir Dakir Damaiyanti, Dian Widya Debby Rosalina Devi Wedayanti, Made Diana Soesilo Diana Soesilo, Diana Dwi Hariyanto Effendy, Aidil Amin Esti Setiawati Eva Lestari Faiser, Adli Febriastuti Cahyani Febriyani Putri, Rizky Fitria Rahmitasari Halimah, Siti Nur Handayani, Budi Handriutomo, Yosef Kevin Hariningtyas Dian Rachmawati Hariyanto, Dwi Hendro, Onge Victoria Hidayanti, Sukma Hidayat, Ridwan Nur indriani, yulis Istiqomah, Layli Nur Khanati, Olivia Kristanti Parisihni Kurniawan, Hansen Kurniawati, Febi Lestari, IGAM Oka Mandasari, Mardyalita Marpaung, Lilis Aprilia Melina Maharani Moh. Basroni Rizal Muhammad Nasrudin Mukhlis Rohmadi Mutika Sari, Mella Nabilah, Putri Nilna Nugroho, Taufik Nuranisa, Nisa Nurcahyono, Eka Nurfasiha, Nurfasiha Nurmala Dewi, Nurmala Pardana Wati, Sri Laksmi Pertiwi, Annisa Mutiara Pranoto, Amelia Elizabeth Pratama, Reza Yogi Priambodo, Nur Tsurayya Puguh Bayu Prabowo, Puguh Bayu Puspita, Sinta Putri Paramita Raharjo, Pambudi Ramadhan, Tri Rachmad Ramadina, Novia Riski Rayhan, Rafialdi Rima Parwati Sari, Rima Parwati Rina Rembah Riski, Mardiyanti Rizal, Mohammad Basroni Sabila, Attina Surya Saptaswari, Dianty Sarianoferni Sekar Purbarini Kawuryan Septiyana, Nadhifa Bunga Setiawan Edi Wibowo Slamet Riyadi Sularsih, S Sundari, Eva Suprihati - Syar, Nur Inayah Teguh Irawan Twi Agnita Cevanti Velisia, Jessica W, Widyastuti Widyastuti Widyastuti Wijayanti, Anggi Karunia Wira, Rizko Yeni Kusumawaty Yogi Aldias Zakariyah Yongki Hadinata Wijaya Yufita Fitriani