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All Journal Jurnal Kedokteran Syiah Kuala Berkala Ilmu Kesehatan Kulit dan Kelamin Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease Majalah Obstetri dan Ginekologi Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya J-Dinamika: Jurnal Pengabdian Kepada Masyarakat YARSI Medical Journal Jurnal Respirasi (JR) JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Jurnal Abdi Insani Majalah Biomorfologi (Biomorphology Journal) Jurnal Layanan Masyarakat (Journal of Public Service) Indian Journal of Forensic Medicine & Toxicology Archives of Pediatric Gastroenterology, Hepatology, and Nutrition Journal of Clinical Microbiology and Infectious Diseases Jurnal Widya Medika Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Eko Budi Koendhori, Eko Budi
Departemen Mikrobiologi Fakultas Kedokteran Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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Journal : JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga

 1 
Effectivity of Cacao Rind Ethanol Extract in Inhibiting Streptococcus Pyogenes Growth In Vitro Cynthia Dwi Ramadhanie; Sri Purwaningsih; Eko Budi Koendhori
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 11 No. 1 (2020): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Faculty of Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V11I12020.6-8

Abstract

Introduction: Infectious disease is still a common cause of illness and death in developing countries, such as Indonesia. One of the bacteria that causes infectious disease is Streptococcus pyogenes. Cacao fruit is a large commodity in Indonesia and has benefit for human. Cacao’s rind is known to contain several active compounds such as flavonoid and alkaloid that have antibacterial effect that can inhibit Streptococcus pyogenes growth. This research aims to evaluate the Minimum Bactericidal Concentration (MBC) of cacao rind ethanol extract in inhibiting Streptococcus pyogenes growth in vitro.  Methods: This research was a laboratory experimental study, testing antibacterial activity of cacao’s rind ethanol extract in inhibiting growth of bacteria Streptococcus pyogenes using dilution method in vitro to know the MIC and MBC result. Sample of bacteria Streptococcus pyogenes was obtained from Balai Besar Laboratorium, Surabaya. Sample of cacao’s rind ethanol extract was extracted at Balai Materia Medica, Batu.  Results: At the beginning this experiment was done to find the MIC and MBC of cacao’s rind ethanol extract against the growth of bacteria Streptococcus pyogenes, but the researcher can only find the MBC result, because the extract color is very dark, so the turbidity result of tubes P1 – P7 cannot be compared to control tube. From the results, the researcher draws a table showing how turbid and dark those tubes are. More (+) signs means more turbid or darker the tube is. From dilution test, the MBC of cacao’s rind ethanol extract against the growth of bacteria Streptococcus pyogenes is 12.5%. Conclusion: Cacao’s rind (Theobroma cacao L.) was quite effective in increasing the growth of bacteria Streptococcus pyogenes in vitro, the Minimum Bactericidal Concentration (MBC) is 12.5%
Ventilator-Associated Pneumonia in Dr. Soetomo General Hospital Surabaya Ismi Masyithah; Usman Hadi; Eko Budi Koendhori
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 12 No. 2 (2021): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Faculty of Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V12I22021.57-60

Abstract

Introduction: Ventilator-associated pneumonia (VAP) is one of the most common nosocomial infections in the intensive care unit (ICU). Mechanically, ventilated patients have a higher risk of VAP. VAP can increase morbidity, mortality, and treatment costs. However, Indonesia lacks data about VAP. The objective of this study was to find out the characteristics of VAP in Dr. Soetomo General Hospital, Surabaya, in the period of August 2017 until August 2018.Methods: This was a cross-sectional study by assessing medical records of the patients in ICU. The variables observed in this study were age, gender, outcome, length of stay in the ICU (LOSICU), and primary diagnosis. The inclusion criteria were the patients who have been using mechanical ventilation at least 2 x 24 hours in the ICU and confirmed VAP. The data were analyzed descriptively by using Microsoft Excel 2016.Results: A total of 18 VAP patients were diagnosed. VAP patients were dominated male (55.6%). The mean of LOSICU was 22.83 ± 11.24 days, and the mortality rate of VAP patients was high (55.6%). 30% of VAP patients had Guillain-Barre Syndrome (GBS) as the primary diagnosis, and 16.65% had Congenital Heart Disease (CHD).Conclusion: Most VAP patients were male and had prolonged stays. The mortality rate of VAP patients was still high. GBS was the most frequent of primary diagnoses in patients with VAP and also CHD.
Effects of Clove Leaf Essential Oil (Syzygium aromaticum) in Inhibiting Biofilm Formation on Candida albicans Isolate Rifai, Achmad; Endraswari, Pepy Dwi; Setiawati, Yuani; Koendhori, Eko Budi
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 15 No. 2 (2024): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V15I22024.70-75

Abstract

Highlights: Candida albicans is capable of forming biofilm, which can lead to resistance to antifungal treatments. Clove leaf essential oil, which can inhibit biofilm formation, making it a potential anti-biofilm agent.   Abstract Introduction: Candida albicans (C. albicans) has a virulence factor, like biofilm formation. Biofilm is a three-dimensional structure that plays a role in antimicrobial resistance, thus requiring antibiofilm agents to address this problem. One of them is Clove leaf (S. aromaticum). S. aromaticum has active phytochemicals (eugenol, β-caryophyllene, and others) that can inhibit biofilm formation in microorganisms, including fungi. This study aimed to prove the effect and to find a concentration of Clove leaf essential oil that affects the biofilm formation of C. albicans isolate. Methods: This study used a microtiter plate with a two-fold dilution technique. The tested concentrations were 6.25%, 3.125%, and 1.5625%. The positive control was 200 µL of C. albicans biofilm suspension, and the negative control was 200 µL of tryptic soy broth media, and all were repeated four times. The biofilm was observed using crystal violet and evaluated using optical density. The data was analyzed statistically using the International Business Machines Corporation (IBM) Statistical Package for the Social Sciences (SPSS) version 26, with a p<0.05 considered statistically significant. Results: The Optical density (O.D.) of the isolate was 2.039, while the negative control was 0.349, indicating that the isolate was a strong biofilm former. The concentrations of 6.25%, 3.125%, and 1.5625% showed inhibition percentages of 8.533%, 17.214%, and 8.484%, respectively. The Optical density was found to be normally distributed but not homogeneous p>0.05, The Kruskal-Wallis’s test was significant, and the Mann-Whitney test was not significant between test groups and positive control. Conclusion: Clove leaf essential oil has inhibitory effects on C. albicans biofilm isolates. However, statistically, there was no significant difference between the test groups and the positive control.
Co-Authors ACHMAD RIFAI Alimsardjono, Lindawati Alpha Fardah Athiyyah Anak Agung Putri Nadia Paramitha Andy Darma Andy Setiawan Arthur Pohan Kawilarang Ayu Lidya Paramitha Bagus Meurah Suropati Bambang Purwanto Betty Agustina Tambunan Catur Endra Arky, Catur Endra Cynthia Dwi Ramadhanie Deby Kusumaningrum Dewi Ratna Sari Dewinta Enggar Pramesthi Dian Neni Naelasari Diani Dwi Indrasari Dimas Firman Hidayat Eddy Bagus Wasito Endraswari, Pepy Evy Ervianti Fernanda Toriq Ainur Rochman Gatut Hardianto, Gatut Hanik Urifah, Hanik Harsono, Setio Henky Mohammad Masteryanto Hermanto Tri Joewono Hidayat, Dimas Firman I Gusti Made Reza Gunadi Ranuh Ikhwani, Irfan Arif Imam Susilo Irfan Arif Ikhwani Ismi Masyithah Jayanti Putri, Jayanti Juita, Liza Puspa Senja Asmara Juniastuti Juniastuti Kadariswantiningsih, Ika Khadijah Rizky Sumitro Kuntaman Kuntaman Kusumaningrumm, Deby Lestari, Aprilia Dwi Linda Dewanti Lindarto, Wira Widjaya M. Amin M. Amin ManikRetno Wahyunitisari Mia Rahardjo Muhammad Aafi Baharuddin Attamimi Muna, Nafdzu Mahmudatul Muna, Nafdzu Makhmudatul Nasicha, Arifatun Ni Made Mertaniasib Ni Made Mertaniasih Ni Njoman Juliasih Nurul Wiqoyah, Nurul Nuswantoro, Djohar Paramitha, Ayu Lidya Pepy Dwi Endraswari, Pepy Dwi Priyo Budi Purwono Putri, Naomi Lesmana Raihan Akbar Muhammad Rakhmatul Binti Sulistya Ratna Kusumawati Ratna Kusumawati Ratna Kusumawati Rebekah Setiabudi, Rebekah Rosantia Sarassari S. Soedarsono Sardjono, Lindawati Alim Sawitri Sawitri Semita, I Nyoman Setiawan, Firman Setya Wijoyo, Dhia Lintang Shazia Hafazhah Aulia Silvia Sutandhio Sri Purwaningsih Steven Christian Susianto Subijanto Marto Sudarmo Sugeng Harijono, Sugeng Terza Aflika Happy Titiek Sulistyowati Titiek Sulistyowati Ummi Maimunah Usman Hadi Vermasari, Naritha Wahyu Setyarini Wahyunitisari, Manik Retno Wardhani, Puspa Widyatama, Fikri Sasongko Wiwin Retnowati Yelvi Levani Yitijuatni Yuani Setiawati Yudayanti, Elprania Credo Yuliati Hood