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Antioxidant and anti-elastase activity of Tampui (Baccaurea macrocarpa) and Ketapong (Terminalia badamia) barks and leaves Ariani, Lusiana; Wulandari, Endah; Desmiaty, Yesi
JURNAL ILMU KEFARMASIAN INDONESIA Vol 22 No 2 (2024): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v22i2.1393

Elastase is a protease that is involved in the breakdown of elastin in the dermis. Likewise, free radicals can cause elastin damage. Inhibition of elastase enzyme activity and scavenging of free radicals can prevent premature aging of the skin, especially wrinkles. The current study was to investigate the antioxidant and elastase inhibition properties of the leaves and bark of the Tampui (Baccaurea macrocarpa L.) and Ketapong (Terminalia badamia L.) plants, which may be employed as an anti-aging ingredient in cosmetics and nutraceuticals. B. macrocarpa and T. badamia's leaves and bark were extracted using reflux and 96% ethanol. Anti-elastase was examined using N-succ-(Ala)3-nitroanilide as a substrate, and the DPPH reagent was used to test the antioxidants' activity. Phytochemical screening indicated the presence of flavonoids, quinone, tannins, and steroids. Antioxidant activity of B. macrocarpa leaves (BML), B. macrocarpa bark (BMB), T. badamia leaves (TBL), and T. badamia bark (TBB) had IC50 values were 15.09±0.51; 22.89±1.51; 36.43±1.94; 39.23±1.76 ppm, respectively. The anti-elastase activity of BML, BMB, TBL, and TBB had IC50 values were 48.86±2.29; 52.03±9.54; 44.42±4.53; 40.16±1.32 ppm, respectively. The ethanol extract of B. macrocarpa and T. badamia's leaves and bark exhibits anti-elastase and antioxidant action, proposing that it may be used as an anti-aging agent.

Activity of hair tonic preparation combining Apium graveolens L extract, Tussilago farfara flower extract, Achillea millefolium extract, Cinchona succirubra bark extract on male New Zealand white rabbits Ardi, Maria Yedida; Faizatun, Faizatun; Desmiaty, Yesi
JURNAL ILMU KEFARMASIAN INDONESIA Vol 22 No 2 (2024): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v22i2.1615

Numerous hair care products are currently available in the market, including shampoo, conditioner, hair mask, hair serum, and hair tonic. Unlike shampoo, hair tonic remains in contact with the scalp for a longer duration, potentially enhancing its effectiveness. Apium graveolens L (EDS) and a combination of Tussilago Farfara flowers, Achillea Millefolium L leaves, and Cinchona Succirubra L stems, marketed as ATG, are known for their hair growth-promoting properties. This study aimed to evaluate the hair growth efficacy, stability, and safety of a hair tonic formulated from EDS and ATG extracts. The prepared extract solution was applied to the shaved backs of male New Zealand rabbits, aged 4 months and weighing 2.5-3 kg, and observations were recorded on days 7, 14, 21, and 28. Parameters measured included hair length and weight. The results indicated that the F1 formulation yielded an average hair length of 9.89 mm and weight of 49.38 mg; F2, 10.36 mm and 49.5 mg; F3, 11.98 mm and 49.5 mg; F4, 17.44 mm and 60.63 mg; F5, 17.27 mm and 52.75 mg; and F6, 19.06 mm and 56.5 mg. Conclusively, the F6 formulation demonstrated the most significant results, with an average hair length of 19.06 mm and weight of 56.5 mg. The hair tonic formulation was found to be non-irritating to the skin and remained stable throughout the study.

In Silico Study of Inhibition of Activation Of 1A52 Ethanol Extract Leunca Fruit (Solanum nigrum L) Fajar, In Rahmi Fatria; Anwar, Effionora; Mumpuni, Esti; Desmiaty, Yesi; Agustini, Kurnia
SANITAS: Jurnal Teknologi dan Seni Kesehatan Vol 15 No 2 (2024): SANITAS Volume 15 Nomor 2 Tahun 2024
Publisher : Politeknik Kesehatan Kemenkes Jakarta II

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36525/sanitas.2024.516

The development of science has found a way to prevent skin aging in menopausal women. One of the therapies is estrogen replacement therapy (17β estradiol). Skin aging in menopausal women is associated with decreased ERα expression. Leunca fruit (Solanum nigrum L) is known to contain phytoestrogens and has the potential as an antiaging agent. The purpose of this study was to predict the anti-aging effect of Solanum nigrum ethanol extract compounds through the Erα 1A52 [A] activation pathway. The research method of active substance analysis metabolite profiling was carried out by analyzing physicochemical properties using pkCSM. This analysis was carried out as an initial selection of compound safety for therapy. Molecular docking with the MVD (Free trial) application. The RMSD value ≤ 2Å indicates a valid docking and the application can be used. The results of the in silico study showed that the results of metabolite profiling showed that diosgenin was an agonist against ERα. The compound has physicochemical properties according to the Lipinski standard so that it can bind to ERα. The conclusion is that diosgenin has the potential and can act as a 1A52 [A] inhibitor so it can be used as an antiaging agent.

Antioxidant and Anti-aging Activities from Zodia (Evodia suaveolens J.R. Forst & G. Forst) Fruit and Leaf Extract Greesty F. Swandiny; Yesi Desmiaty; Haura Usna Rahmah
Jurnal Jamu Indonesia Vol. 7 No. 1 (2022): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v7i1.169

Aging is a natural process that occurs in every living organism, including humans. Skin wrinkle, caused by deterioration activity of the elastase enzyme, is one example of the aging process. The activity of the elastase enzyme could be inhibited by flavonoid and phenolic compounds. Zodia (Evodia suaveolens) from the family of Rutaceae is one of the indigenous Indonesian plants widely known as rich in flavonoid and phenolic compounds. The purpose of this study is to explore antioxidant and anti-aging activity from ethanolic extract of fruit and leaf Zodia. The extracts were prepared by refluxing fruit and leaf zodia in 96% ethanol. The obtained extracts were characterized for the phytochemical constituent and its antioxidant activity determined using the 2,2-diphenyl-1-picrylhydrazyl method as well as anti-elastase activity for anti-aging evaluation. Phytochemical analysis of both samples showed the presence of flavonoids, saponins, tannins, steroids and essential oils. The calculation of IC50 in antioxidant activity using the DPPH method showed that ethanolic extract of zodia leaf (264.15 ppm) was higher compared to fruit extract (121.59 ppm). The fruit extract was further tested for anti elastase activity with IC50 of 145.67 ppm. In conclusion, the presence of phytochemicals in zodia fruit may be responsible for the antioxidant and anti-aging activities.

Phenolic Content and Antioxidant Activity of Colocasia esculenta in Commercial Herbal Products Nugroho, Gumilar Adhi; Desmiaty, Yesi; Sumiyati, Yati; Suherman, Sandra Loretta; Lim, Helsa
Sciences of Phytochemistry Volume 4 Issue 1
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/sciphy0401288

Taro (Colocasia esculenta L.) is a plant with a long history of use in traditional medicine in several countries worldwide. The corm of taro and two commercial products were analysed due to total phenolic, flavonoid, luteolin, and antioxidant properties. Taro corm was extracted with water and analysed for phytochemical screening, total flavonoids, total phenolic, luteolin, and antioxidant properties. The phytochemical screening of taro showed that it contains secondary metabolites of flavonoids, tannins, saponins, triterpenoids, and quinones. The results of the determination of total flavonoid content of taro extract, Product 1, and Product 2 capsule obtained 19.34 ± 0.26 mg QE/g extract, 18.58 ± 0.18 mg QE/g extract, and 16.29 ± 0.23 mg QE/g extract. The results of determining the total phenolic content of taro, Product 1, and Product 2 capsules obtained 39.75 ± 0.95 mg GAE/g extract, 38.46 ± 0.46 mg GAE/g extract, and 35.91 mg GAE/g extract. ± 0.82 mg GAE/g extract. The results of the determination of luteolin from taro, Product 1, and Product 2 capsules obtained 0.83 ± 0.05 %, 0.39 ± 0.04 %, and 0.18 ± 0.08 %. The results of the antioxidant activity test obtained IC50 of vitamin C of 4.6780 ± 0.0319 ppm, corm dry extract and ginger rhizome 141.2383 ± 3.4995 ppm and 97.6685 ± 7.8760 ppm, respectively, and capsule products 2; 126.0724± 1.3672 ppm. The water extract of corm and available products containing CE extract have met the quality standard parameters required for non-specific parameters, luteolin content, and antioxidant properties.

ANALISIS PREDIKSI EFEK ANTIINFLAMASI PADA PIPER PORPHYROPHYLLUM SECARA IN SILICO Prayoga, Tria; Rahmat, Deni; Sandhiutami, Ni Made Dwi; Desmiaty, Yesi
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 10 No 1 (2025): Jurnal Ilmiah Ibnu Sina
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jiis.v9i2.2352

This study aims to predict the anti-inflammatory effects of compounds in Piper porphyrophyllum using in silico methods. The compounds were analyzed using AutoDock software to evaluate their binding potential with the target protein and Lipinski's rule of five to assess their drug-likeness based on pharmacokinetic properties. Qualitative results show that compounds such as 4'-hydroxy-3',5,7-trimethoxyflavone, 4',5-dihydroxy-3',7-dimethoxyflavone, and 5-hydroxy-3',4',7-trimethoxyflavone form significant interactions with key residues on the target protein. Quantitatively, these compounds exhibit more negative binding free energy compared to the natural ligand (-7.34 kcal/mol), with values of -8.35 kcal/mol, -8.13 kcal/mol, and -8.20 kcal/mol, respectively, indicating stronger binding affinity. Evaluation using Lipinski's rule shows that these compounds meet the criteria as potential drug candidates. In conclusion, this in silico method can predict the potential of Piper porphyrophyllum compounds as anti-inflammatory agents, supporting further research for experimental validation.

Enhanced Anti-Inflammatory Activity of Passiflora edulis Leaf Extract Nanoparticle Gel in Carrageenan-Induced Rat Model Sandhiutami, Ni Made Dwi; Desmiaty, Yesi; Fahleni, Fahleni; Handayani, Rizqya Cahya; Ciang, Windy
Sciences of Pharmacy Volume 4 Issue 1
Publisher : ETFLIN Publishing House

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58920/sciphar0401301

Passion fruit (Passiflora edulis) leaf extract is rich in flavonoids, which possess anti-inflammatory properties. This study aimed to enhance the anti-inflammatory effect of the extract through a nanoparticle gel formulation. Nanoparticles of the extract (NEP) were prepared using ionic gelation with a Carbomer 940 base. The anti-inflammatory efficacy of NEP was evaluated in vivo using a carrageenan-induced paw edema model in rats. Five groups (n=5) were tested: a negative control (Carbomer 940 gel base), a positive control (Sodium Diclofenac), and three NEP formulations with concentrations of 1% (NEP-1), 3% (NEP-2), and 5% (NEP-3). The NEP gels were homogeneous with a faint yellow color and a characteristic odor. Their spreadability ranged from 7.50 ± 0.67 cm to 7.69 ± 1.26 cm, viscosity exhibited plastic flow properties, and pH values were within 4.47 ± 0.06 to 5.20 ± 0.10. The anti-inflammatory effectiveness of NEP formulations increased with concentration, achieving edema inhibition rates of 3.66% (NEP-1), 68.47% (NEP-2), and 82.37% (NEP-3). In conclusion, the nanoparticle gel formulations of passion fruit leaf extract demonstrated good physical properties, with NEP-3 being the most effective in reducing carrageenan-induced paw edema in rats.

Formulation of a peel-off gel mask containing mundu fruit extract (Garcinia dulcis (roxb.) kurz) for antioxidant and anti-elastase activity Ambarwati, Neneng Siti Silfi; Desmiaty, Yesi; Chandra, Amelia; Noviani, Yuslia; Triyani, Yulia; Hakim, Ratu Mayra; Amanda, Reistha Fazlia; Wahyudi, Dion
JURNAL ILMU KEFARMASIAN INDONESIA Vol 23 No 1 (2025): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v23i1.1607

The skin serves as a protective barrier against ultraviolet (UV) radiation. However, exposure to UV radiation can lead to various skin problems, including changes in the dermis. To prevent UV-induced damage, antioxidants and anti-elastase agents are needed to protect the skin from oxidative stress. This study aims to evaluate the antioxidant and anti-elastase activities of Mundu fruit extract (Garcinia dulcis (Roxb.) Kurz) and to formulate a peel-off gel mask using the concentrated extract. Antioxidant activity was assessed using the FRAP and ABTS methods, while anti-elastase activity was determined by measuring the release of p-nitroaniline from a substrate degraded by the elastase enzyme. The results showed that the IC50 value for antioxidant activity using the ABTS method is 29.57 ppm 0.72, and the FRAP value was 185.02 10.23 AAE/g. The anti-elastase activity showed IC50 of 82.21 ppm. Three peel-off gel mask formulations were developed, with Formula 3 (containing extract at 200 x IC50). showing the best performance. These findings suggest that the thick Mundu fruit extract, which tested positive for phenolic compounds, exhibits promising antioxidant and anti-elastase activities and is suitable for use in topical formulations.

Novel Enhalus acoroides Phytosomes: Formulation, Characterization, and Bioavailability Enhancement Siti Mardiyanti; Effionora Anwar; Yesi Desmiaty; Siti Sadiah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 7 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i7.1333

Background: Enhalus acoroides (seagrass) possesses valuable bioactive compounds, including quercetin, with potential therapeutic applications, notably antidiabetic effects. However, the poor solubility and low bioavailability of compounds like quercetin limit their clinical efficacy. Phytosomes, phospholipid-based nanocarriers, represent a promising strategy to overcome these limitations. This study aimed to develop and characterize E. acoroides extract-loaded phytosomes to enhance its potential bioavailability. Methods: E. acoroides was collected, processed, and extracted using ultrasound-assisted extraction (UAE). Total phenolic (TPC) and flavonoid content (TFC) were determined. Phytosomes were prepared using the thin-layer hydration method with varying extract-to-soya lecithin ratios (F1=1:1, F2=1:2, F3=1:3). Characterization involved particle size analysis, zeta potential measurement, Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM), entrapment efficiency (EE%) determination via HPLC, and in vitro dissolution studies. Results: The UAE extract yielded TPC of 0.318 ± 0.036 mg GAE/g and TFC of 1.023 ± 0.022 mg QE/g. Phytosome formulation F1 (1:1 ratio) exhibited optimal characteristics: particle size of 276.4 nm, PDI of 0.591, zeta potential of -18.0 mV, EE% of 80.47 ± 2.62%, and a spherical morphology. FTIR confirmed complexation. F1 phytosomes demonstrated significantly enhanced dissolution, releasing 87.13% of the entrapped compound over 12 hours compared to the crude extract. Conclusion: E. acoroides extract was successfully encapsulated into phytosomes using a thin-layer method. The F1 formulation (1:1 extract:phospholipid ratio) demonstrated favorable physicochemical properties (nanoparticle size, moderate stability, high EE%) and markedly improved in vitro dissolution, suggesting enhanced bioavailability potential for E. acoroides phytoconstituents.

Novel Enhalus acoroides Phytosomes: Formulation, Characterization, and Bioavailability Enhancement Siti Mardiyanti; Effionora Anwar; Yesi Desmiaty; Siti Sadiah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 7 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i7.1333

Background: Enhalus acoroides (seagrass) possesses valuable bioactive compounds, including quercetin, with potential therapeutic applications, notably antidiabetic effects. However, the poor solubility and low bioavailability of compounds like quercetin limit their clinical efficacy. Phytosomes, phospholipid-based nanocarriers, represent a promising strategy to overcome these limitations. This study aimed to develop and characterize E. acoroides extract-loaded phytosomes to enhance its potential bioavailability. Methods: E. acoroides was collected, processed, and extracted using ultrasound-assisted extraction (UAE). Total phenolic (TPC) and flavonoid content (TFC) were determined. Phytosomes were prepared using the thin-layer hydration method with varying extract-to-soya lecithin ratios (F1=1:1, F2=1:2, F3=1:3). Characterization involved particle size analysis, zeta potential measurement, Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM), entrapment efficiency (EE%) determination via HPLC, and in vitro dissolution studies. Results: The UAE extract yielded TPC of 0.318 ± 0.036 mg GAE/g and TFC of 1.023 ± 0.022 mg QE/g. Phytosome formulation F1 (1:1 ratio) exhibited optimal characteristics: particle size of 276.4 nm, PDI of 0.591, zeta potential of -18.0 mV, EE% of 80.47 ± 2.62%, and a spherical morphology. FTIR confirmed complexation. F1 phytosomes demonstrated significantly enhanced dissolution, releasing 87.13% of the entrapped compound over 12 hours compared to the crude extract. Conclusion: E. acoroides extract was successfully encapsulated into phytosomes using a thin-layer method. The F1 formulation (1:1 extract:phospholipid ratio) demonstrated favorable physicochemical properties (nanoparticle size, moderate stability, high EE%) and markedly improved in vitro dissolution, suggesting enhanced bioavailability potential for E. acoroides phytoconstituents.

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