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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Jurnal Ilmu Komputer dan Agri-Informatika Jurnal Hortikultura JURNAL AGROTEKNOLOGI AGRIVITA, Journal of Agricultural Science Jurnal Agro Journal of Tropical Biodiversity and Biotechnology Caraka Tani: Journal of Sustainable Agriculture Communications in Science and Technology Jurnal Penelitian Kelapa Sawit Journal of Tropical Crop Science Nusantara Science and Technology Proceedings Jurnal Penelitian Inovatif International Journal of Agriculture System Jejak: Jurnal Pendidikan Sejarah Universitas Jambi Policy Brief Pertanian, Kelautan, dan Biosains Tropika
Willy Bayuardi Suwarno
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Perbaikan Tata Kelola Perberasan Nasional Helmi, Alfian; Sitanggang, Azis Boing; Suwarno, Willy Bayuardi; Ramadhani, Nabilah Zulfah; Anggraeny, Bunga; Firmansyah, Muh
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol 6 No 1 (2024): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0601.834-837

Abstract

Sebagai salah satu pangan pokok rakyat Indonesia, kenaikan harga beras yang terjadi belakangan ini tentunya memberikan dampak yang signifikan dan respon yang beragam. Secara lebih sederhana, penyebab kenaikan harga beras di hampir seluruh wilayah negeri dapat dilihat dari neraca supply dan demand-nya.
Identification of Lines Inducing Male Sterility in Hybrid Maize Farid, Muh; Azrai, Muhammad; Nur, Amin; Efendy, Roy; Salengke, Salengke; Musa, Yunus; Baharuddin, Baharuddin; Kuswinanti, Tutik; Thamrin, Sulaeha; Suwarno, Willy Bayuardi; Anshori, Muhammad Fuad; Fadhli, Nur; Andayani, Nining; Z, Bunyamin; Mirsam, Hishar; Priyanto, Slamet Bambang; Suriani, Suriani; Novianti, Fira
International Journal of Agriculture System VOLUME 11 ISSUE 2, DECEMBER 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v11i2.4994

Abstract

Efficiency and effectiveness in controlling crossbreeding are pivotal in yielding superior hybrid varieties with desired traits such as genetic purity and high productivity. Cytoplasmic Male Sterile (CMS) is a crucial component in hybrid variety assembly. This study aims to identify the genotypes that can induce male sterile lines based on genetic diversity and the level of pollen sterility in the prospective male sterile genotypes tested, consisting of seventeen genotypes. The research findings revealed that the genetic diversity among the seventeen observed male-sterile candidate genotypes tends to vary in quantitative traits. Among the quantitative traits, JHD14 exhibited indications of being a male sterility inducer compared to the other genotypes observed, demonstrating a value of 0.00 g in terms of pollen weight. This is further substantiated by the microscopic examination of JHD14 pollen grains, which displayed 100% sterility. 
Flowering Phenology and SiDREB2-based SNAP Marker-assisted Hybridity Confirmation for Artificial Hybridization of Indonesian Foxtail Millet (Setaria italica L. Beauv) Genotypes Nugroho, Rizki Bagus; Chusnah, Uswatun; Suwarno, Willy Bayuardi; Ardie, Sintho Wahyuning
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1082-1094

Abstract

Foxtail millet hybrid development encounters challenges due to its self-pollinating nature, small florets, and tool availability for confirming true hybrid. Our research aimed to assess flowering phenology and explore artificial hybridization employing SiDREB2 based-SNAP marker. The research consisted of three experiments. The first experiment focused on the panicle phenology of foxtail millet, while the second experiment focused on floret phenology and reproductive organs. The artificial hybridization successfulness was validated with SNAP markers based on the SiDREB2 gene in the third experiment. Observations on two genotypes (ICERI-5 and ICERI-6) revealed panicle initiation at 49–69 days after planting (DAP), with anthesis occurring 6 days after panicle emergence (DAPE). Florets exhibited the formation of reproductive organs at 3 DAPE, delineating distinct stages of pollen development leading up to anthesis. Our findings emphasized the significance of implementing male-sterile induction treatment at 4 DAPE to regulate pollen development for successful hybridization by 6 DAPE. The hybrid evaluation revealed varied germination rates and confirmed hybrid percentages across different crossing series, validated by SiDREB2-based SNAP marker. ICERI-5×Botok-4 exhibited high germination but a low confirmed hybrid percentage, while Botok-4×ICERI-5 and Botok-10×ICERI-6 showed the opposite trend. Reciprocal crossings showed unilateral incompatibility in confirmed hybrid percentage.
Resistance of Doubled Haploid Rice Lines with Green Super Rice Characters to Bacterial Leaf Blight Siti Nurhidayah; Bambang Sapta Purwoko; Iswari Saraswati Dewi; Willy Bayuardi Suwarno; Iskandar Lubis; Siti Yuriyah
Caraka Tani: Journal of Sustainable Agriculture Vol 39, No 2 (2024): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v39i2.88198

Abstract

Bacterial leaf blight (BLB), caused by Xanthomonas oryzae pv. oryzae (Xoo) is a significant disease attacking rice crops worldwide. This disease attacks at various stages of plant growth and causes significant yield loss. Breeding new varieties resistant to BLB is important to support sustainable agriculture in the future. This study aimed to identify new superior green super rice (GSR) lines resistant to BLB disease. The experiment evaluated the resistance of lowland rice lines obtained from anther culture using a factorial randomized complete block design. The 1st factor was genotype, consisting of 20 lines, 2 checks of commercial varieties (Inpari 42 Agritan GSR and Inpari 18), a resistant check (Conde), and a susceptible check (Taichung Native 1). The 2nd factor was BLB pathotypes (i.e., III, IV, and VIII). Quantitative data on disease severity and severity index were analyzed using analysis of variance and t-Dunnett test at 5% level. The results showed that the interaction between genotype and pathotype affected the disease severity and severity index in both growth phases. The tested lines exhibited varying resistance, from susceptible to resistant, to BLB. Four lines (SN 11, 13, 57, and 58) showed moderate to resistant criteria for BLB disease of 3 pathotypes in both growth phases. The selected lines can be used as a source of parents for breeders and candidates for new superior varieties with BLB resistance properties to support the reduction of synthetic chemical bactericide inputs and control BLB disease. However, further field evaluations are necessary to assess their performance.
Development of Microsatellite Markers to Determine Genetic Diversity of Indonesian Betel Nut (Areca catechu L.) Muhammad Roiyan Romadhon; Sobir; Willy Bayuardi Suwarno; Deden Derajat Matra
Nusantara Science and Technology Proceedings Multi-Conference Proceeding Series D
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3002

Abstract

Areca nut (Areca catechu) belongs to the monocotyledonous and palm family of plants known as betel nut or supari plants. The Center of origin areca nut comes from India. Areca nut is a cross-pollinated plant with a heterozygous heterogeneous genetic constitution. Next-generation sequencing is widely used for plant breeding activities, including developing SSR markers with an in-silico approach. This research aims to develop an SSR marker to detect genetic diversity in local areca nut from Indonesia. The genomic data used to develop SSR markers were obtained from the Whole Genome Sequencing of Pinang Reyan Hainan Cultivars obtained from NCBI. Fragments containing SSR regions were isolated using MISA software, and primers were designed with Primer3web v.4.1.0 (https://bioinfo.ut.ee/primer3/). The average total size of the sequences tested was 168770731.8, with a total number of SSRs identified as many as 1149,813. SSR motifs are used as a step in genetic diversity research because these motifs will be used in primary attachments which in turn can affect genetic diversity. There were twenty-eight primers generated from four amplicons (100-150, 150-200, 200-250, and 250-300 bp) used for multiplex primers with motifs (AAT)37, (TA)53, (TA)54, (TC)52, (TC)52, (TG)53, and (AG)54.
Analisis Single Nucleotide Polymorphism (SNP) Gen Laccase-24 (EgLCC24) dalam Ketahanan Kelapa Sawit (Elaeis guineensis Jacq.) terhadap Ganoderma boninense Nugroho, Syarul; Yuli Rahmadi, Hernawan; Syukur, Muhamad; Widodo, Widodo; Bayuardi Suwarno, Willy; Sri Wening, Sri Wening; Faizah, Rokhana; Diah Setiowati, Retno; Adriwan Siregar, Heri
Jurnal Penelitian Kelapa Sawit Vol 33 No 1 (2025): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v33i1.285

Abstract

Ganoderma boninense attack and its rapid spread in oil palm plantations have caused significant economic losses. The use of Ganoderma resistant plant materials can be a solution, but the allelic segregation that occurs causes not all DxP progeny obtained have the nature of resistance to Ganoderma. The role of molecular markers is needed to assist initial selection of Ganoderma resistant DxP progenies in nurseries. This study aims to identify targeted SNP markers from the Laccase-24 gene (EgLCC24), which is thought to play a role in oil palm resistance to Ganoderma. Three oil palm DxP populations were used in this study, namely populations A, B, and C. Ganoderma screening in nurseries was carried out on these three populations to obtain progeny samples with resistant and susceptible phenotypes, which were then further analyzed. The methods used include SNP analysis and the use of SNAP primers for marker development. The SNP differences obtained from this study led to amino acid changes, but did not cause stop codons. The validation results using SNAP primers on the Laccase-24 gene showed that DxP progeny from populations A, B, and C relatively have moderate allele characteristics of Ganoderma resistance.
Early Identification of Potentially Drought-Tolerant Doubled Haploid Rice Lines During the Seedling Stage Munandar, Arief; Purwoko, Bambang Sapto; Dewi, Iswari Saraswati; Suwarno, Willy Bayuardi; Hadianto, Wira; Nurhidayah, Siti
Journal of Tropical Crop Science Vol. 12 No. 02 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.02.346-357

Abstract

Climate change and reduced crop yields caused by drought stress have increased the demand for drought-tolerant varieties. The anther culture technique allows the production of improved varieties with high homozygosity in a short time. The study aimed to select the drought-tolerant double haploid lines at the seedling stage. This research was conducted in the greenhouse of the Indonesian Center for Agricultural Biotechnology and Genetic Resources Instrument Standard Testing (BBPSI Biogen), Bogor, from August to November 2023. The drought-tolerant selection of 12 doubled-haploid rice lines (AE1-AE12) and four check varieties, i.e., two commercial checks (“Inpari 18” Tadah Hujan Agritan or AE13, “Bioni63” Ciherang Agritan or AE14), one drought-tolerant check (“Salumpikit” or AE15), and one drought-sensitive check (“IR20” or AE16). The research used a randomized complete block design with three replications. The characters observed were leaf rolling, leaf drying, recovery ability, and plant fresh and dry weight. The Friedman test results showed that the lines with the lowest rankings, three lines (AE2, AE5, and AE12) with mild tolerant criteria for leaf rolling, five lines (AE1, AE2, AE5, AE8, and AE12) with mild tolerant criteria for leaf drying, and three lines (AE1, AE5, and AE12) with tolerant criteria for recovery ability. The selection index for drought tolerance at the seedling stage identified seven lines with positive values. Based on the Friedman test, selection index, and heatmap visualization, AE12, AE1, AE5, and AE8 exhibited a tolerance similar to “Salumpikit” and were deemed suitable based on drought tolerance characters.
Analysis of drought stress tolerance in doubled haploid lines of green super rice at the vegetative stage Nurhidayah, Siti; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Suwarno, Willy Bayuardi; Lubis, Iskandar; Hadianto, Wira; Munandar, Arief
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 2 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i2.63893

Abstract

Current climate change has caused drought in various regions, which has decreased rice yields. Green super rice (GSR) has the characteristic of being tolerant to water limitation. GSR lines were bred through anther culture technique to obtain doubled haploid (DH) plants. This study aimed to analyze the response of DH GSR lines to drought stress at the vegetative stage and identify potential lines based on the weighted selection index. The experiment was arranged in a randomized complete block design with three replications. The materials used consisted of twenty DH lines, Inpari 42 Agritan GSR, and Inpari 18 as check varieties, Salumpikit as a drought-tolerant check, and IR 20 as a drought-sensitive check. Qualitative data were analyzed using the non-parametric Friedman test. The result showed different responses among the lines for leaf rolling, leaf drying, and recovery ability. Line SN14 exhibited moderate leaf drying and showed improvement to mild tolerance during the recovery phase. Nine lines (SN12, 14, 32, 40, 51, 57, 58, 59, and 60) were selected using a selection index based on leaf rolling, leaf drying, and recovery ability. These lines can be further tested for drought tolerance tests until the reproductive stage, and the tolerant lines could be useful for future development. Keywords: abiotic stress; anther culture; climate change; selection index; water limitation
Identification of Lines Inducing Male Sterility in Hybrid Maize Farid, Muh; Azrai, Muhammad; Nur, Amin; Efendy, Roy; Salengke, Salengke; Musa, Yunus; Baharuddin, Baharuddin; Kuswinanti, Tutik; Thamrin, Sulaeha; Suwarno, Willy Bayuardi; Anshori, Muhammad Fuad; Fadhli, Nur; Andayani, Nining; Z, Bunyamin; Mirsam, Hishar; Priyanto, Slamet Bambang; Suriani, Suriani; Novianti, Fira
International Journal of Agriculture System VOLUME 11 ISSUE 2, DECEMBER 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v11i2.4994

Abstract

Efficiency and effectiveness in controlling crossbreeding are pivotal in yielding superior hybrid varieties with desired traits such as genetic purity and high productivity. Cytoplasmic Male Sterile (CMS) is a crucial component in hybrid variety assembly. This study aims to identify the genotypes that can induce male sterile lines based on genetic diversity and the level of pollen sterility in the prospective male sterile genotypes tested, consisting of seventeen genotypes. The research findings revealed that the genetic diversity among the seventeen observed male-sterile candidate genotypes tends to vary in quantitative traits. Among the quantitative traits, JHD14 exhibited indications of being a male sterility inducer compared to the other genotypes observed, demonstrating a value of 0.00 g in terms of pollen weight. This is further substantiated by the microscopic examination of JHD14 pollen grains, which displayed 100% sterility. 
Membangun Karakter Profil Pelajar Pancasila Berbasis Kearifan Lokal dengan Perspektif Kebudayaan Bali Brata, Ida Bagus; Sartika, Lianda Dewi; Saputra, I Putu Adi
Jurnal Penelitian Inovatif Vol 4 No 3 (2024): JUPIN Agustus 2024
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jupin.338

Abstract

Proses pembelajaran sepanjang hayat merupakan salah satu upaya mengembangkan potensi peserta didik berbasis nilai kearifan lokal Bali sangat mendukung terwujudnya profil pelajar Pancasila. Tujuan dari kajian ini adalah untuk menganalisis nilai kearifan lokal perspektif kebudayaan Bali dalam membangun profil pelajar Pancasila sebagai bagian dari kurikulum merdeka belajar untuk mengarahkan peserta didik agar menghargai dan mencintai budayanya sendiri. Metode dalam penelitian ini menggunakan metode penelitian kualitatif dengan pendekatan studi literatur (literature review). Pendekatan studi literatur dilakukan dengan cara membaca, mencatat, dan menelaah bahan penelitian melalui berbagai sumber yang terkait dengan tujuan dan masalah penelitian melalui data pendukung yang bersumber dari buku, hasil lokakarya, prosiding, jurnal penelitian baik nasional maupun internasional dan sumber-sumber lainnya seperti internet. Hasil kajian menemukan bahwa nilai kearifan lokal perspektif kebudayaan Bali dapat dijadikan salah satu strategi untuk menciptakan paradigma baru dalam mewujudkan kompetensi global menuju profil karakter siswa Pancasila. Kearifan lokal merupakan salah satu aspek budaya yang dimiliki daerah-daerah termasuk Bali sangat banyak dan beraneka bentuknya, secara selektif dapat diangkat menjadi aset nasional dan dapat direvitalisasi sehingga dapat difungsikan sebagai wahana untuk membangun profil pelajar Pancasila.
Co-Authors , Krisantini , Supartopo , Suwarno Abdul Qadir Adlan Adriwan Siregar, Heri Agus Purwito Ajitama, Trio Fanny Akbar, Miftahur Rizqi Alfian Helmi Amalia Nurul Huda AMIN NUR Amin Nur Amy Estiati Anas Dinurrohman Susila Andayani, Nining Anggara, Heru Anggita Duhita Anindyajati Anggraeny, Bunga Anshori, Muhammad Fuad Aprilianti, Devi Kurnia Aris Hairmansis Awang Maharijaya Ayub Darmanto Azis Boing Sitanggang Azrai, dan Muhammad Badrieh, Haian Amin Baharuddin Baharuddin Bambang Sapta Purwoko Bambang Suprihatno Bayu Pramono Wibowo Budi Indra Setiawan Butarbutar, Lidya Kristina Sari Chusnah, Uswatun Chusnul Arif Constantin, Mondjeli Cucu Gunarsih da Cunha, Rojino Danu Kuncoro Deden Derajat Matra Desi Anugra Safitri Dewi Sukma Dewi, dan Iswari Saraswati Diah Setiowati, Retno Dian Rakhmad Diny Dinarti Efendi, Darda Efendy, Roy Ekasari Putri, Nurwanita Endang Gunawan Eny Widajati Fadhli, Nur Farid, Muh Fidya Novita Firmansyah, Muh HAJRIAL ASWIDINNOOR Hishar Mirsam I Made Legawa I Putu Adi Saputra, I Putu Indrastuti A. Rumanti Indrastuti Apri Rumanti Iskandar Lubis Iskandar Lubis Iswari Saraswati Dewi Iswari Saraswati Dewi Iswari Saraswati Dewi Jayaningsih, Elvita Dwi Juharni Jundi Aiman Abdullah Kusuma, I Gede Artha Surya Kuswandi Kuswandi Lianda Dewi Sartika Lopes Hornai, Ermelinda Maria Maharijaya, dan Awang Matsuda, Hiroshi Maya Melati Mohammad Syafii Muhamad Askari Muhamad Syukur Muhammad Azrai, Muhammad Muhammad Jauhar Firdaus Muhammad Roiyan Romadhon Munandar, Arief Munif Ghulamahdi Nafisah Nailatul Karomah Ni Made Armini Wiendi Nindita, Anggi Novianti, Fira Nugroho, Rizki Anjal Puji Nugroho, Rizki Bagus Nugroho, Syarul Nurul Khumaida Priyanto, Slamet Bambang Purwoko, Bambang Sapta Purwoko Purwoko, Bambang Sapto Rahmah, Syifaur Ramadaniarto Rizqullah Ramadhan, Fitrah Ramadhani, Nabilah Zulfah Reswari, Helvi Ardana Ridwani, Sobir Rika Miftakhul Jannah Rina Hapsari Wening, Rina Hapsari Rokhana Faizah rudiyanto Rusianto Safitri, dan Heni Salengke, Salengke Sani, Mutiyara Sari, Dewi Citra Sari, Siti Hapita Satyanto Krido Saptomo Sherly Rahayu Sintho Wahyuning Ardie Siti Nurhidayah Siti Nurhidayah Siti Yuriyah Slamet Susanto Sobir Sobir Sobir Sri Ratnawati Sri Wahjuni Sri Wening Sri Wening, Sri Wening Sugiyanta, dan Suriani Suriani Surjono Hadi Sutjahjo Susila, Anas Dinnurohman Suwarno, Punjung Medaraji Syah, Uswah Trywulan Syaifullah Rahim Syawaluddin, Dwi Dana Syilvia Nosya Pratama Tamura, Koremasa THAMRIN, SULAEHA TRI HASTINI Trias Sitaresmi Trikoesoemaningtyas Tutik Kuswinanti Umi Salamah Untung Susanto Wage Ratna Rohaeni Wida W. Khumaero Widodo Widodo Wijaya, Aldi Kamal Winarso D. Widodo Wira Hadianto, Wira Yudiwanti Wahyu E. Kusumo Yuli Irvana Dewi Yuli Rahmadi, Hernawan YUNUS MUSA Z, Bunyamin Zakiah, Rizqi Alifahasni