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Perbaikan Tata Kelola Perberasan Nasional Helmi, Alfian; Sitanggang, Azis Boing; Suwarno, Willy Bayuardi; Ramadhani, Nabilah Zulfah; Anggraeny, Bunga; Firmansyah, Muh
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol 6 No 1 (2024): Policy Brief Pertanian, Kelautan dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0601.834-837

Abstract

Sebagai salah satu pangan pokok rakyat Indonesia, kenaikan harga beras yang terjadi belakangan ini tentunya memberikan dampak yang signifikan dan respon yang beragam. Secara lebih sederhana, penyebab kenaikan harga beras di hampir seluruh wilayah negeri dapat dilihat dari neraca supply dan demand-nya.
Identification of Lines Inducing Male Sterility in Hybrid Maize Farid, Muh; Azrai, Muhammad; Nur, Amin; Efendy, Roy; Salengke, Salengke; Musa, Yunus; Baharuddin, Baharuddin; Kuswinanti, Tutik; Thamrin, Sulaeha; Suwarno, Willy Bayuardi; Anshori, Muhammad Fuad; Fadhli, Nur; Andayani, Nining; Z, Bunyamin; Mirsam, Hishar; Priyanto, Slamet Bambang; Suriani, Suriani; Novianti, Fira
International Journal of Agriculture System VOLUME 11 ISSUE 2, DECEMBER 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v11i2.4994

Abstract

Efficiency and effectiveness in controlling crossbreeding are pivotal in yielding superior hybrid varieties with desired traits such as genetic purity and high productivity. Cytoplasmic Male Sterile (CMS) is a crucial component in hybrid variety assembly. This study aims to identify the genotypes that can induce male sterile lines based on genetic diversity and the level of pollen sterility in the prospective male sterile genotypes tested, consisting of seventeen genotypes. The research findings revealed that the genetic diversity among the seventeen observed male-sterile candidate genotypes tends to vary in quantitative traits. Among the quantitative traits, JHD14 exhibited indications of being a male sterility inducer compared to the other genotypes observed, demonstrating a value of 0.00 g in terms of pollen weight. This is further substantiated by the microscopic examination of JHD14 pollen grains, which displayed 100% sterility. 
Flowering Phenology and SiDREB2-based SNAP Marker-assisted Hybridity Confirmation for Artificial Hybridization of Indonesian Foxtail Millet (Setaria italica L. Beauv) Genotypes Nugroho, Rizki Bagus; Chusnah, Uswatun; Suwarno, Willy Bayuardi; Ardie, Sintho Wahyuning
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1082-1094

Abstract

Foxtail millet hybrid development encounters challenges due to its self-pollinating nature, small florets, and tool availability for confirming true hybrid. Our research aimed to assess flowering phenology and explore artificial hybridization employing SiDREB2 based-SNAP marker. The research consisted of three experiments. The first experiment focused on the panicle phenology of foxtail millet, while the second experiment focused on floret phenology and reproductive organs. The artificial hybridization successfulness was validated with SNAP markers based on the SiDREB2 gene in the third experiment. Observations on two genotypes (ICERI-5 and ICERI-6) revealed panicle initiation at 49–69 days after planting (DAP), with anthesis occurring 6 days after panicle emergence (DAPE). Florets exhibited the formation of reproductive organs at 3 DAPE, delineating distinct stages of pollen development leading up to anthesis. Our findings emphasized the significance of implementing male-sterile induction treatment at 4 DAPE to regulate pollen development for successful hybridization by 6 DAPE. The hybrid evaluation revealed varied germination rates and confirmed hybrid percentages across different crossing series, validated by SiDREB2-based SNAP marker. ICERI-5×Botok-4 exhibited high germination but a low confirmed hybrid percentage, while Botok-4×ICERI-5 and Botok-10×ICERI-6 showed the opposite trend. Reciprocal crossings showed unilateral incompatibility in confirmed hybrid percentage.
Development of Microsatellite Markers to Determine Genetic Diversity of Indonesian Betel Nut (Areca catechu L.) Muhammad Roiyan Romadhon; Sobir; Willy Bayuardi Suwarno; Deden Derajat Matra
Nusantara Science and Technology Proceedings Multi-Conference Proceeding Series D
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3002

Abstract

Areca nut (Areca catechu) belongs to the monocotyledonous and palm family of plants known as betel nut or supari plants. The Center of origin areca nut comes from India. Areca nut is a cross-pollinated plant with a heterozygous heterogeneous genetic constitution. Next-generation sequencing is widely used for plant breeding activities, including developing SSR markers with an in-silico approach. This research aims to develop an SSR marker to detect genetic diversity in local areca nut from Indonesia. The genomic data used to develop SSR markers were obtained from the Whole Genome Sequencing of Pinang Reyan Hainan Cultivars obtained from NCBI. Fragments containing SSR regions were isolated using MISA software, and primers were designed with Primer3web v.4.1.0 (https://bioinfo.ut.ee/primer3/). The average total size of the sequences tested was 168770731.8, with a total number of SSRs identified as many as 1149,813. SSR motifs are used as a step in genetic diversity research because these motifs will be used in primary attachments which in turn can affect genetic diversity. There were twenty-eight primers generated from four amplicons (100-150, 150-200, 200-250, and 250-300 bp) used for multiplex primers with motifs (AAT)37, (TA)53, (TA)54, (TC)52, (TC)52, (TG)53, and (AG)54.
Analisis Single Nucleotide Polymorphism (SNP) Gen Laccase-24 (EgLCC24) dalam Ketahanan Kelapa Sawit (Elaeis guineensis Jacq.) terhadap Ganoderma boninense Nugroho, Syarul; Yuli Rahmadi, Hernawan; Syukur, Muhamad; Widodo, Widodo; Bayuardi Suwarno, Willy; Sri Wening, Sri Wening; Faizah, Rokhana; Diah Setiowati, Retno; Adriwan Siregar, Heri
Jurnal Penelitian Kelapa Sawit Vol 33 No 1 (2025): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v33i1.285

Abstract

Ganoderma boninense attack and its rapid spread in oil palm plantations have caused significant economic losses. The use of Ganoderma resistant plant materials can be a solution, but the allelic segregation that occurs causes not all DxP progeny obtained have the nature of resistance to Ganoderma. The role of molecular markers is needed to assist initial selection of Ganoderma resistant DxP progenies in nurseries. This study aims to identify targeted SNP markers from the Laccase-24 gene (EgLCC24), which is thought to play a role in oil palm resistance to Ganoderma. Three oil palm DxP populations were used in this study, namely populations A, B, and C. Ganoderma screening in nurseries was carried out on these three populations to obtain progeny samples with resistant and susceptible phenotypes, which were then further analyzed. The methods used include SNP analysis and the use of SNAP primers for marker development. The SNP differences obtained from this study led to amino acid changes, but did not cause stop codons. The validation results using SNAP primers on the Laccase-24 gene showed that DxP progeny from populations A, B, and C relatively have moderate allele characteristics of Ganoderma resistance.
Early Identification of Potentially Drought-Tolerant Doubled Haploid Rice Lines During the Seedling Stage Munandar, Arief; Purwoko, Bambang Sapto; Dewi, Iswari Saraswati; Suwarno, Willy Bayuardi; Hadianto, Wira; Nurhidayah, Siti
Journal of Tropical Crop Science Vol. 12 No. 02 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.02.346-357

Abstract

Climate change and reduced crop yields caused by drought stress have increased the demand for drought-tolerant varieties. The anther culture technique allows the production of improved varieties with high homozygosity in a short time. The study aimed to select the drought-tolerant double haploid lines at the seedling stage. This research was conducted in the greenhouse of the Indonesian Center for Agricultural Biotechnology and Genetic Resources Instrument Standard Testing (BBPSI Biogen), Bogor, from August to November 2023. The drought-tolerant selection of 12 doubled-haploid rice lines (AE1-AE12) and four check varieties, i.e., two commercial checks (“Inpari 18” Tadah Hujan Agritan or AE13, “Bioni63” Ciherang Agritan or AE14), one drought-tolerant check (“Salumpikit” or AE15), and one drought-sensitive check (“IR20” or AE16). The research used a randomized complete block design with three replications. The characters observed were leaf rolling, leaf drying, recovery ability, and plant fresh and dry weight. The Friedman test results showed that the lines with the lowest rankings, three lines (AE2, AE5, and AE12) with mild tolerant criteria for leaf rolling, five lines (AE1, AE2, AE5, AE8, and AE12) with mild tolerant criteria for leaf drying, and three lines (AE1, AE5, and AE12) with tolerant criteria for recovery ability. The selection index for drought tolerance at the seedling stage identified seven lines with positive values. Based on the Friedman test, selection index, and heatmap visualization, AE12, AE1, AE5, and AE8 exhibited a tolerance similar to “Salumpikit” and were deemed suitable based on drought tolerance characters.
Identification of Lines Inducing Male Sterility in Hybrid Maize Farid, Muh; Azrai, Muhammad; Nur, Amin; Efendy, Roy; Salengke, Salengke; Musa, Yunus; Baharuddin, Baharuddin; Kuswinanti, Tutik; Thamrin, Sulaeha; Suwarno, Willy Bayuardi; Anshori, Muhammad Fuad; Fadhli, Nur; Andayani, Nining; Z, Bunyamin; Mirsam, Hishar; Priyanto, Slamet Bambang; Suriani, Suriani; Novianti, Fira
International Journal of Agriculture System VOLUME 11 ISSUE 2, DECEMBER 2023
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijas.v11i2.4994

Abstract

Efficiency and effectiveness in controlling crossbreeding are pivotal in yielding superior hybrid varieties with desired traits such as genetic purity and high productivity. Cytoplasmic Male Sterile (CMS) is a crucial component in hybrid variety assembly. This study aims to identify the genotypes that can induce male sterile lines based on genetic diversity and the level of pollen sterility in the prospective male sterile genotypes tested, consisting of seventeen genotypes. The research findings revealed that the genetic diversity among the seventeen observed male-sterile candidate genotypes tends to vary in quantitative traits. Among the quantitative traits, JHD14 exhibited indications of being a male sterility inducer compared to the other genotypes observed, demonstrating a value of 0.00 g in terms of pollen weight. This is further substantiated by the microscopic examination of JHD14 pollen grains, which displayed 100% sterility. 
Membangun Karakter Profil Pelajar Pancasila Berbasis Kearifan Lokal dengan Perspektif Kebudayaan Bali Brata, Ida Bagus; Sartika, Lianda Dewi; Saputra, I Putu Adi
Jurnal Penelitian Inovatif Vol 4 No 3 (2024): JUPIN Agustus 2024
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jupin.338

Abstract

Proses pembelajaran sepanjang hayat merupakan salah satu upaya mengembangkan potensi peserta didik berbasis nilai kearifan lokal Bali sangat mendukung terwujudnya profil pelajar Pancasila. Tujuan dari kajian ini adalah untuk menganalisis nilai kearifan lokal perspektif kebudayaan Bali dalam membangun profil pelajar Pancasila sebagai bagian dari kurikulum merdeka belajar untuk mengarahkan peserta didik agar menghargai dan mencintai budayanya sendiri. Metode dalam penelitian ini menggunakan metode penelitian kualitatif dengan pendekatan studi literatur (literature review). Pendekatan studi literatur dilakukan dengan cara membaca, mencatat, dan menelaah bahan penelitian melalui berbagai sumber yang terkait dengan tujuan dan masalah penelitian melalui data pendukung yang bersumber dari buku, hasil lokakarya, prosiding, jurnal penelitian baik nasional maupun internasional dan sumber-sumber lainnya seperti internet. Hasil kajian menemukan bahwa nilai kearifan lokal perspektif kebudayaan Bali dapat dijadikan salah satu strategi untuk menciptakan paradigma baru dalam mewujudkan kompetensi global menuju profil karakter siswa Pancasila. Kearifan lokal merupakan salah satu aspek budaya yang dimiliki daerah-daerah termasuk Bali sangat banyak dan beraneka bentuknya, secara selektif dapat diangkat menjadi aset nasional dan dapat direvitalisasi sehingga dapat difungsikan sebagai wahana untuk membangun profil pelajar Pancasila.
Respon Delapan Genotipe Melon (Cucumis melo L.) terhadap Perlakuan KNO3 Huda, Amalia Nurul; Suwarno, Willy Bayuardi; Maharijaya, Awang
Jurnal Hortikultura Indonesia (JHI) Vol. 9 No. 2 (2018): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.337 KB) | DOI: 10.29244/jhi.9.2.84-92

Abstract

ABSTRACTPotassium (K) is a mineral nutrient needed by crops that influences the quality of fruits and vegetables. The objective of this study was to elucidate the interaction effects of genotype by KNO3 treatment (G × KNO3) on fruit traits of melon. The experiment was conducted from August to October 2016 at Tajur II Experimental Station of IPB, Bogor, following a split plot design with three replicates. The main plot was two levels of KNO3 and the subplot was eight genotypes, consisting of seven test genotypes and one check variety (Eagle). The KNO3 treatments were applied weekly, started from 7-49 DAP with 5 g L-1 concentration, ±200 ml plant-1. G × KNO3 interaction effects were significant for sugar content and titratable acidity (TA). P34 had relatively high sugar content and TA. P25 showed a significant increase of sugar content when KNO3 is added, while Eagle, P311, and P34 showed significant decrease of sugar content. Days to male flowering, days to hermaphrodite flowering, and fruit weight had high broad sense heritability (repeatability), while days to harvest, fruit diameter, flesh thickness, rind thickness, and sugar content had moderate heritability. Fruit weight had significant positive correlation with fruit diameter, flesh thickness, and rind thickness. Application of KNO3 fertilizer in practical is therefore suggested for the postive-response genotypes to KNO3.Key words: fruit quality, KNO3, melon, sugar contentABSTRAKKalium (K) merupakan mineral yang dibutuhkan oleh tanaman yang berpengaruh terhadap kualitas buah dan sayur. Pada budidaya melon umumnya, sumber mineral K diperoleh dalam bentuk KNO3. Penelitian ini bertujuan mengidentifikasi pengaruh interaksi genotipe × perlakuan KNO3 (G × KNO3) terhadap peningkatan kualitas buah melon. Percobaan dilakukan pada bulan Agustus sampai Oktober 2016 di Kebun Percobaan IPB Tajur II, Bogor menggunakan rancangan petak terbagi (split plot design) dengan tiga ulangan. Petak utama merupakan perlakuan KNO3 dengan dua taraf, dan anak petak merupakan genotipe dengan delapan taraf, yang terdiri dari tujuh genotipe uji dan satu varietas pembanding (Eagle). Perlakuan KNO3 dilakukan setiap minggu mulai 7–49 HST dengan konsentrasi 5 g L-1 dan volume aplikasi ± 200 ml tanaman-1. Interaksi G × KNO3 berpengaruh nyata terhadap karakter kadar gula dan total asam tertitrasi (TAT). Genotipe P34 memiliki kadar gula dan TAT yang relatif tinggi. P25 merupakan genotipe yang menunjukkan respon positif berupa peningkatan kadar gula pada perlakuan KNO3, namun sebaliknya genotipe Eagle, P311, dan P34 justru menunjukkan penurunan kadar gula yang signifikan pada perlakuan KNO3. Karakter yang memiliki nilai heritabilitas (repeatabilitas) arti luas yang tergolong tinggi adalah umur berbunga jantan, umur berbunga hermaprodit, dan bobot buah, sedangkan yang heritabilitasnya tergolong sedang adalah umur panen, diameter buah, tebal daging buah, tebal kulit buah, dan kadar gula. Bobot buah berkorelasi positif dengan diameter buah, tebal daging buah, dan tebal kulit buah. Aplikasi pemupukan KNO3 secara praktis disarankan pada genotipe melon yang memiliki respon positif terhadap KNO3.Kata kunci: kandungan gula, KNO3, kualitas buah, melon
Evaluasi Genotipe Melon (Cucumis melo L.) untuk Perakitan Varietas Hibrida Baru Jundi Aiman Abdullah; Suwarno, Willy Bayuardi; Yudiwanti Wahyu Endro Kusumo
Jurnal Hortikultura Indonesia (JHI) Vol. 14 No. 1 (2023): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.14.1.56-62

Abstract

Indonesia merupakan negara produsen serta konsumen buah melon (Cucumis melo L.) yang tinggi, tetapi impor benih melon dari luar negeri masih cukup besar. Hal tersebut membuka peluang pemulia tanaman untuk merakit varietas hibrida unggul. Varietas melon hibrida yang diharapkan adalah yang memiliki kualitas buah yang baik dan produksi yang tinggi. Penelitian ini bertujuan mempelajari karakteristik beberapa genotipe melon dan memilih genotipe potensial untuk materi perakitan varietas hibrida baru. Genotipe yang digunakan ialah enam genotipe dari koleksi Pusat Kajian Hortikultura Tropika (PKHT) IPB (IPB G1, IPB G30, IPB G41, IPB 240, IPB 283, Glamour-S1), tiga galur inbrida PKHT IPB (IPB M13, IPB M23, IPB M21), dan satu varietas pembanding (Alisha F1). Genotipe IPB G1 dan IPB 240 memiliki keunggulan penampakan buah menarik dengan keunikan berupa corak pada kulit buah genotipe IPB G1 dan jala yang tebal dengan intensitas tidak terlalu rapat pada genotipe IPB 240. Genotipe IPB G41 memiliki kandungan padatan terlarut total yang tinggi dan rasanya sangat manis. Genotipe Glamour-S1 memiliki keunggulan penampakan buah menarik serta buah yang besar. Karakter bobot buah berkorelasi positif dengan diameter buah dan panjang buah. Kandungan PTT berkorelasi positif dengan umur panen. Berdasarkan analisis korelasi, genotipe potensial adalah yang memiliki ukuran buah yang besar dan umur panen yang cukup. Genotipe yang memiliki potensi sebagai materi pemuliaan adalah IPB G1, IPB 240, IPB G41, dan Glamour-S1. Kata kunci: genotipe potensial, karakteristik melon, pemuliaan melon
Co-Authors , Krisantini , Supartopo , Suwarno Abdul Qadir Adlan Adriwan Siregar, Heri Agus Purwito AHMAD JUNAEDI Ajitama, Trio Fanny Akbar, Miftahur Rizqi Alfian Helmi Amalia Nurul Huda AMIN NUR Amin Nur Amy Estiati Anas Dinurrohman Susila Andayani, Nining Anggara, Heru Anggita Duhita Anindyajati Anggraeny, Bunga Anshori, Muhammad Fuad Aprilianti, Devi Kurnia Aris Hairmansis Awang Maharijaya Ayub Darmanto Azis Boing Sitanggang Azrai, dan Muhammad Badrieh, Haian Amin Baharuddin Baharuddin Bambang Sapta Purwoko Bambang Suprihatno Bayu Pramono Wibowo Budi Indra Setiawan Butarbutar, Lidya Kristina Sari Chusnah, Uswatun Chusnul Arif Constantin, Mondjeli Cucu Gunarsih Cucu Gunarsih da Cunha, Rojino Danu Kuncoro Deden Derajat Matra Desi Anugra Safitri Dewi Sukma Dewi, dan Iswari Saraswati Diah Setiowati, Retno Dian Rakhmad Diny Dinarti Dwi Arizna Arofah Efendi, Darda Efendy, Roy Ekasari Putri, Nurwanita Endang Gunawan Eny Widajati Fadhilah Sitepu, Annisa Fadhli, Nur Farid, Muh Fidya Novita Firmansyah, Muh HAJRIAL ASWIDINNOOR Hajrial Aswidinnoor Hishar Mirsam I Made Legawa I Putu Adi Saputra, I Putu Indrastuti A. Rumanti Indrastuti Apri Rumanti Iskandar Lubis Iswari Saraswati Dewi Iswari Saraswati Dewi Jayaningsih, Elvita Dwi Juharni Jundi Aiman Abdullah Kusuma, I Gede Artha Surya Kuswandi Kuswandi Lianda Dewi Sartika Lopes Hornai, Ermelinda Maria Maharijaya, dan Awang Matsuda, Hiroshi Maya Melati Mohammad Syafii Muhamad Askari Muhamad Syukur Muhammad Azrai, Muhammad Muhammad Jauhar Firdaus Muhammad Roiyan Romadhon Munandar, Arief Munif Ghulamahdi Nafisah Nailatul Karomah Natasya Nafila Ni Made Armini Wiendi Nindita, Anggi Novianti, Fira Nugroho, Rizki Anjal Puji Nugroho, Rizki Bagus Nugroho, Syarul Nurul Khumaida Pahlevi, M Reza Priyanto, Slamet Bambang Purwoko, Bambang Sapta Purwoko Purwoko, Bambang Sapto Rahmah, Syifaur Ramadaniarto Rizqullah Ramadhan, Fitrah Ramadhani, Nabilah Zulfah Reswari, Helvi Ardana Ridwani, Sobir Rika Miftakhul Jannah Rina Hapsari Wening, Rina Hapsari Rizqullah, Ramadaniarto Rokhana Faizah rudiyanto Rusianto Saenal, Nirwanti Safitri, dan Heni Salengke, Salengke Sani, Mutiyara Sari, Dewi Citra Sari, Siti Hapita Satyanto Krido Saptomo Sherly Rahayu Sintho Wahyuning Ardie Siti Nurhidayah Siti Yuriyah Slamet Susanto Sobir Sobir Sobir Sri Ratnawati Sri Wahjuni Sri Wening Sri Wening, Sri Wening Sugiyanta, dan Suriani Suriani Surjono Hadi Sutjahjo Susila, Anas Dinnurohman Suwarno, Punjung Medaraji Syah, Uswah Trywulan Syaifullah Rahim Syawaluddin, Dwi Dana Syilvia Nosya Pratama Tamura, Koremasa THAMRIN, SULAEHA TRI HASTINI Trias Sitaresmi Trikoesoemaningtyas Tsugama, Daisuke Tutik Kuswinanti Umi Salamah Untung Susanto Wage Ratna Rohaeni Wida W. Khumaero Widodo Widodo Wijaya, Aldi Kamal Winarso D. Widodo Wira Hadianto Wospakrik, Andrean Heskiel Yuana, Hayu Widi Yudiansyah Yudiansyah Yudiwanti Wahyu E. Kusumo Yuli Irvana Dewi Yuli Rahmadi, Hernawan YUNUS MUSA Z, Bunyamin Zakiah, Rizqi Alifahasni