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Determination of specific and non-specific standardization parameters for ethanol extract of purple leaves (Graptophyllum pictum (L) Griff ) Rivani Tampang; Syafika Alaydrus; Niluh Puspita Dewi; Joni Tandi
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.8812

Abstract

this study aims to determine the standardization of specific parameters (organoleptic and chemical content) and non-specific parameters (ash content, water content, drying loss, heavy metal contamination, Yeast Mold Number (AKK), microbial contamination / Total Plate Count (ALT)). The results showed that the specific parameters of the organoleptic properties of 96% ethanol extract of purple leaves (Grapthophyllum pictum (L) Griff) were black, shaped like a paste, a distinctive purple leaf odor and a bitter taste. The results of phytochemical screening of 96% purple leaf ethanol extract contained alkaloids, flavonoids, and tannins. However, the saponin test was negative for saponins. Non-specific parameters of 96% ethanol extract of cherry leaves were water content of 5.96%, total ash content of 16.86%, drying loss of 17.26%, heavy metals As and Cd were not detected, the presence of metals in Pb was 0.12 mg / kg, microbial contamination (Alt) and yeast mold numbers (Akk) were not found colonies. Based on these results, it can be concluded that the standardization test of specific and non-specific parameters, the ethanol extract of purple leaves has met the quality standards of simple drugs.
MACROSCOPIC AND MICROSCOPIC GASTROPROTECTIVE ACTIVITY OF MUCOADHESIVE GRANULE FORMULATIONS OF CLOVE LEAF ETHANOL EXTRACT Dhitania Ivana Harliwong; Ayu Wulandari; Niluh Puspita Dewi; Sririzqi Muthmainnah; Joni Tandi; Putu Intan
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.8949

Abstract

Gastric ulcers can be triggered by excessive HCl secretion, and the regenerative capacity of mucosal cells can be weakened by high alcohol levels and anti-inflammatory drugs, including NSAIDs. Gastrin functions to stimulate the secretion of gastric juice, particularly HCl and pepsinogen This study aims to evaluate the macroscopic and microscopic gastroprotective activity of clove leaf ethanol extract mucoadhesive granule formulations. The study used 25 rats divided into five groups: normal control, negative control, positive control, clove leaf ethanol extract group, and mucoadhesive granule formulation of clove leaf ethanol extract group. All groups except the normal control were administered acetylsalicylic acid. Treatments were conducted over 9 days, with surgical examination and observations performed macroscopically and microscopically on day 10. Macroscopic assessments revealed ulcer damage improvement in the positive control, extract, and mucoadhesive granule groups, all scored at 1. Microscopic assessments also indicated a significant difference between the negative control and the positive control, extract, and mucoadhesive granule groups, showing better gastric repair, scored at 0.5. Therefore, it can be concluded that the administration of the mucoadhesive granule formulation of clove leaf ethanol extract exhibits gastroprotective activity comparable to that of the clove leaf ethanol extract.
Acute Toxicity Test of Gastroretentive Mucoadhesive Granule Preparation of Earthworm Flour (Lumbricus rubellus) Magfirah; Novalia Merue; Joni Tandi; Utami Islamiati
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.8952

Abstract

Earthworms (Lumbricus rubellus) contain the compound lumbricin, which is beneficial for treatment, and its use is still simple, leading to the innovation of gastroretentive mucoadhesive granule formulations. However, its security has not yet been fully supported by research. This research aims to determine the toxic effects caused by earthworm granule preparations in terms of toxicity symptoms and the determination of the LD50 value. This research used 20 male white rats that were randomly divided into 4 groups. One control group was given Aquadest, and three treatment groups were given earthworm granules at doses of 500 mg/kgBB (P1), 2000 mg/kgBB (P2), and 5000 mg/kgBB (P3) with a single oral administration. The research results indicate that the preparation of earthworm granules (Lumbricus rubellus) causes toxic symptoms such as decreased locomotor activity, piloerection, and increased grooming frequency in line with the increase in dosage, but does not result in the death of the test animals. (LD50). Based on the research findings, it can be concluded that the LD50 value of the earthworm granules (Lumbricus rubellus) is >5000 mg/kgBB and falls into the practically non-toxic category with an LD50 range of 5–15 g/kgBB
Test of the Effectiveness of Earthworm Flour (Lumbricus rubellus) Gastroretentive Mucoadhesive Granule Formulation on Male White Rats (Rattus norvegicus) Infected with Salmonella typhi Magfirah; Stely Gracia Bengkati; Joni Tandi; Viani Anggi
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.8953

Abstract

Lumbricus rubellus contains lumbricin which is efficacious for treating typhoid fever. The aim of the research was to determine the effectiveness of gastroretentive mucoadhesive granule preparations from earthworm flour. This study used 30 male Wistar rats which were divided into 6 groups which were given orally, the normal and negative control groups were given Na-CMC, the positive control group was given chloramphenicol, and the treatment group was given doses of 200 mg/kgBW, 400 mg/kgBW and 800 mg/kgBW. mg/kgBB gastroretentive mucoadhesive granules earthworm flour. The results of body temperature measurements, on day 7 the control group obtained results of ±37.08, ±39.00 and ±38.09 respectively, and the treatment group obtained results of ±38.09, ±38.09 and ±38.07 respectively. On day 21, the control group obtained results of ±37.08, ±39.00 and ±38.09, respectively, and the treatment group obtained results of ±38.09, ±38.09 and ±38.07, respectively. The Widal test results on the 7th day were positive and after 14 days of administering the preparation on the 21st day the results were negative. It was concluded that the effective dose for typhoid fever therapy was a dose of 400 mg/kgBW.
Efektivitas Ekstrak Daun Gedi Merah terhadap Glukosa Darah, Malondialdehid, 8-Hdroksi-Deoksiguanosin, Insulin Tikus Diabetes Joni Tandi; Muthi’ah H Z; Yuliet Yuliet; Yusriadi Yusriadi
Journal of Tropical Pharmacy and Chemistry Vol. 3 No. 4 (2016): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v3i4.114

Abstract

Red Gedi Leaves Extract (RGLE) have phytochemicals such as alkaloids, flavonoids, polyphenols, saponins and tannins which known as antidiabetic agents. Study had been done about RGLE (Abelmoschus manihot (L.) Medic) on blood glucose level, 8-hydroxy-deoksiguanosin, malondialdehyde and insulin in diabetic rats (Rattus norvegicus). This study aims to prove the effects of RGLE in blood glucose levels, 8-hydroxy-deoksiguanosin, and malondialdehyde and insulin levels in rats with diabetes induced by streptozotocin and which is the most effective dosage of RGLE. Study used pre and post test design. RGLE obtained by maceration method using by ethanol 96%. There were 120 white male rats which were divided into six treatment groups, each group consisting of 20 individuals. Group I (healthy control), II (negative control) were given by Na-CMC 0.5%. Group III as the positive control were given by glibenclamide, group IV, V, and VI given by RGLE with different doses (150, 300 and 450)mg/kg BW. Animal test were diabetic induced by Streptozotocin with 40 mg/kg BW dosage i.p. Data were analyzed by One Way (ANOVA). The result showed that RGLE can reduced blood glucose levels, 8-hydroxy-deoksiguanosin, and malondialdehyde and increased insulin levels in diabetic rats. The Effective Dosage of RGLE is 150 mg/kg BW. Keywords : RGLE(Red Gedi Leaves Extract),Glukose,8-hidroksideoksiguanosin, Malondialdehyde, Insulin. ABSTRAK EDGM (Ekstrak Daun Gedi Merah) mengandung alkaloid, flavonoid, polifenol, saponin, dan tanin diduga mempunyai efek antidiabetes. Telah dilakukan penelitian dari EDGM (Abelmoschus manihot (L.) Medik) terhadap kadar glukosa darah (KGD), 8-hidroksi-deoksiguanosin, malondialdehid, insulin pada tikus putih (Rattus norvegicus) diabetes yang diinduksi streptozotocin telah dilakukan. Tujuan penelitian ini untuk membuktikan kemampuan EDGM terhadap KGD, 8-hidroksi-deoksiguanosin, dan malondialdehid, insulin pada tikus putih diabetes diinduksi streptozotocin dan menentukan dosis paling efektif. Rancangan penelitian digunakan adalah pre and post test design. EDGM diperoleh dengan cara metode etanol 96%. Penelitian ini menggunakan hewan uji tikus putih jantan sebanyak 120 ekor yang dibagi menjadi 6 kelompok perlakuan, tiap kelompok terdiri dari 20 ekor. Kelompok kontrol I (sehat), II negatif(-) diberikan suspensi Na-CMC 0,5%, III positif(+) diberikan suspensi glibenklamid, kelompok IV, V, dan VI diberikan EDGM masing-masing dengan dosis 150, 300 dan 450 mg/kgBB. Model hewan uji diabetes dibuat dengan cara induksi streptozotocin dengan dosis 40 mg/kgBB secara intraperitoneal. Data yang diperoleh dianalisis menggunakan uji statistik one way (ANOVA) pada taraf kepercayaan 95%. Berdasarkan hasil penelitian menunjukkan bahwa ekstrak daun gedi merah dapat menurunkan KGD, 8-hidroksi-deoksiguanosin, malondialdehid dan meningkatkan kadar insulin pada tikus putih diabetes yang diinduksi streptozotocin. Dosis EDGM yang efektif yaitu dosis 150 mg/kg BB. Kata Kunci: EDGM(Ekstrak DaunGediMerah),Glukosa Darah,8-Hidroksideoksiguanosin, Malondialdehid, insulin.
Efektivitas Antihiperurisemia Ekstrak Etanol Daun Seledri (EEDS) pada Tikus Induksi Kalium Oksonat Yasinta Rakanita; Hastuti L; Joni Tandi; Sri Mulyani
Journal of Tropical Pharmacy and Chemistry Vol. 4 No. 1 (2017): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v4i1.124

Abstract

Seledri adalah tanaman yang memiliki kandungan kimia seperti alkaloid, flavonoid, saponin, dan tanin. Penelitian ini bertujuan untuk membuktikan efektivitas ekstrak etanol daun seledri dalam menurunkan kadar asam urat pada tikus putih jantan dan menentukan dosis ekstrak daun seledri yang efektif dalam menurunkan kadar asam urat pada tikus putih jantan. Ekstrak daun seledri dibuat secara maserasi dengan pelarut etanol 96%. Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok. Data yang diperoleh dianalisis dengan mengunakan uji statistik Analisis Sidik Ragam pada taraf kepercayaan 95% yang mengunakan 30 ekor tikus putih jantan dibagi 6 kelompok perlakuan, tiap perlakuan terdiri dari 5 ekor. Model hewan dibuat hiperurisemia menggunakan penginduksi kalium oksonat dengan dosis 250 mg/kg BB. Kelompok I (normal) diberikan pakan standar, kelompok II (negatif) diberi suspensi Na CMC 0,5%, kelompok III (positif) diberi suspensi allopurinol 5,4 mg/kg BB, kelompok IV, V, dan VI diberi ekstrak etanol daun seledri masing-masing dengan dosis 50 mg/kg BB, 100 mg/kg BB, dan 200 mg/kg BB. Berdasarkan uji lanjut BNJ diperoleh hasil bahwa dosis ekstrak etanol daun seledri yang efektif adalah 50 mg/kg BB.
Efek Nefroprotektif Kombinasi Ekstrak Daun Gedi Merah dan Daun Kumis Kucing pada Tikus Induksi Etilen Glikol Joni Tandi; Moh. Roem; Yuliet Yuliet
Journal of Tropical Pharmacy and Chemistry Vol. 4 No. 1 (2017): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v4i1.129

Abstract

Nephroprotective compound is a compound that has the ability to protect the kidneys from kidney disorders caused by free radicals. This research aimed to determine the nephroprotective effect of the combination of Abelmoschus manihot leaves and Orthosiphon stamineus leaves extract as well as to know the effective extract dose combinations as nephroprotective. This research applied Group Random Design with 6 treatment groups consists of 5 tested animals. Group I was normal control. Group II was negative control. All extract groups was given 0,75% of ethylene glycol and 2% of ammonium chloride. Group III was given 100 mg/kg BW of Abelmoschus manihot leaves extract and 200 mg/kg BW of Orthosiphon stamineus leaves extract. Group IV was given 50 mg/kg BW of Abelmoschus manihot leaves extract and 100 mg/kg BW of Orthosiphon stamineus leaves extract. Group V was given 100 mg/kg BW of Abelmoschus manihot leaves extract. Group VI was given 200 mg/kg BW of Orthosiphon stamineus leaves extract. Treatment was given for 14 days and the levels of creatine and urea were measured on day 0, 8 and 15. The results show the nephroprotective effect in all the extract groups, but the most effective one was the group III.
Pengaruh Ekstrak Etanol Daun Jambu Air (Syzygium aqueum (Burm f.)Alston) terhadap Glukosa Darah, Ureum dan Kreatinin Tikus Putih (Rattus norvegicus) Joni Tandi
Journal of Tropical Pharmacy and Chemistry Vol. 4 No. 2 (2017): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v4i2.137

Abstract

Diabetes mellitus with complications of Diabetic Nephropathy become one of the most common cause of end-stage renal failure in the world. Impaired kidney function is measured by Glomerular Filtration Rate (GFR). Where the decline in GFR was followed by increased levels of blood glucose, urea and creatinine. This study aimed to determine whether there is the content of secondary metabolites in leaves of Sizygium aqueum leaf extract, at a dose of extract what impact the blood glucose, urea and creatinine levels of male rats and whether the water extract of guava leaves have an influence on the blood glucose, urea and creatinine white male rats. This study uses a randomized block design with six treatment groups consisting of five test animals. Group I was normal control (CMC Na suspension 0.5%). Group II negative control (streptozotocin 40 mg/kg). positive control group III (suspension glibenclamide). Group IV given guava leaf extract 100 mg/kg BW. V group awarded guava leaf extract 200 mg/kg BW. Group VI given guava leaf extract 300 mg/kg BW. Treatment was given for 28 days and measured levels of blood glucose urea and creatinine on days 0, 7, 14, 21 and 28. The results showed nefroprotektif effect on all groups and most effective extract are a group of guava leaf extract 100 mg/kg BW). Keywords: Diabetic nephropathy, urea, creatinine, Syzygium aqueum
Test of the Potential of Ethanol Extracts, Simplician Medium and Forest Umbi Juice (Eleutherine bulbosa (Factory) Urb.) against Blood Glucose Levels of Rats, and Histopatology Ratkreas Rat (Rattus norvegicus) Hypercolesterolemia Model Diabetic Joni Tandi; Tien Wahyu Handayani; Ni Wayan Ika Purwasih
Journal of Tropical Pharmacy and Chemistry Vol. 5 No. 2 (2020): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v5i2.229

Abstract

This study aims to examine the presence or absence of secondary metabolite compounds in ethanol extract, simplicia steeping and forest onion tuber juice, in male glucose parameters and pancreatic histopathology. Using test animals as many as 30 male white rats divided into 6 groups and each group consisted of 5 rats with group I details as normal controls, group II as negative controls given 0.5% Na-CMC suspension, group III as positive control who were given Metformin 45 mg / kg BB and IV, V, VI as the test group were given ethanol extract, simplicia extract and forest onion tuber juice with each dose of 20 g / kg BW. The results showed that there were secondary metabolites in the ethanol extract of forest onion tubers, namely flavonoids, saponins, alkaloids, steroids and tannins, giving ethanol extract of forest onion bulbs had the effect of reducing blood glucose levels in white mice with a dose of 20g / kgBW with an average of 132 and ethanol extract of forest onion bulbs at a dose of 20g / kg BB with an average of 0.5 can regenerate pancreatic tissue.
Formulasi Tabir Surya Zink Oksida Dalam Sediaan Krim Dengan Variasi Konsentrasi Ekstrak Anggur Hitam (Vitis vinivera L.) Joni Tandi; Kurnia Gatot Novrianto
Jurnal Sains dan Kesehatan Vol. 1 No. 7 (2017): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v1i7.72

Abstract

Senyawa tabir surya yang umum digunakan adalah bahan sintesis, akan tetapi kadangkala bahan sintesis ini dapat menjadi berbahaya bila terserap ke dalam aliran darah. Untuk menghindari dampak negatif tersebut dapat digunakan tanaman obat tradisional, salah satunya anggur hitam. Telah dilakukan penelitian dengan tujuan untuk mengetahui seberapa besar efektivitas nilai SPF tabir surya yang diberikan oleh ekstrak anggur hitam sebagai factor pelindung sinar matahari terhadap kulit. Penelitian ini dilakukan dengan cara mengekstrak anggur hitam dengan metode maserasi bertingkat untuk menarik komponen kimia sebagai senyawa tabir surya. Ekstrak dibuat dalam empat konsentrasi kemudian diukur serapannya menggunakan spektrofotometer UV-Vis pada panjang gelombang 280 nm sampai 400 nm, selanjutnya dihitung SPF. Hasil penelitian menunjukkan dari semua konsentrasi ekstrak anggur hitam memberikan nilai SPF yaitu pada konsentrasi 0% 2,33, konsentrasi 0,4% 2,38, konsentrasi 0,8% 2,48 dan konsentrasi 1,2% 2,53. Dari hasil ini dapat disimpulkan bahwa ekstrak anggur hitam mempunyai efek tabir surya karena nilai SPF yang diperoleh masuk dalam range syarat tabir surya yaitu 2 sampai 60.
Co-Authors Afriani S Afrizal Agustinus Widodo Alaydrus, Syafika Alfia Dewi Anita Purwantari Asrifa Asrifa Astuti, Dewi Qurrotul Ayu Martina Ayu Wulandari AYU WULANDARI Ayu Wulandari Azwaajum Mutahharah Basilingan, Aprince Bella Melinda Cinda Pradita Sudar Claresta, Jong A. Darmayanti David Pakaya David Pakaya Debora Rislianti Lakiu Dewi Astuti Dhitania Ivana Harliwong Dian S. Waruwu Diana Pratiwi, Franciska Erdy Prasetyo Fahruddin Fahruddin Faisal Rahmat Nugraha Fajar Alan Feiverin Tibe Ficanata Adhiguna Toding Gabriella Bamba Ratih Lintin Gusti Ayu Gusti Ayu, Gusti Handayani KR Handayani, Tien Wahyu Handayani, Tien Wahyuni Hastuti L I Made Dipayana Ida Yanti Palinggi Irwan Irwan Islamiati, Utami Jong A. Claresta Kiki Rizki Handayani Kurnia Gatot Novrianto L, Wahyudin H Lakiu, Debora Rislianti Lexy Kareba Lidongi, Putri Marcelita Lina Hardianti Rukmana M, Yulan Magfira Magfira Magfirah Magfirah, Magfirah Magfiroh Magfiroh Maria Kanan Mariyani Marsella, Mimi Marzela Dewi Meike Rachmawati Moh Rizky Moh. Roem Muhammad Agus Muljanto Muthi’ah H Z Na'i, Alpina Nadila Nadila Nadira Nani Astria Ni Made Irma Mariani Ni Putu Intan Riani Ni Putu Setiawati Ni Wayan Ika Purwasih Niluh Puspita Dewi Niluh Puspita Dewi Niluh Puspita Dewi Niluh Puspita Dewi, Niluh Puspita Niswatulfahriyati Niswatulfahriyati Novalia Merue Nur Azizah Nur Ikhwan Nurhikmah Nurhikmah Nurmadinah Nurmadinah Nurul Ulzmi Ovan Nugroho Pegia Parobe, Alda Iglesias Pasang, Sisilia Baan Puspita Dewi, Niluh Putu Intan R, Yana Novika Rahmawati D Rasmiyanti, Ni Kadek Evy Recky Patala Rezky Yanuarty Rio Mariani Risna Yanti Rivani Tampang Rizaldy Lalu Ronaldy Nobertson Ruterlin, Valen Salsabila, Unik Hanifah Sasdila, Sasdila Seblin Tonapa Rammang Solehah, Puput Octaviani Sri Mulyani Sri Mulyani Sri Mulyani Sri Mulyani Sabang Sririzqi Muthmainnah Stely Gracia Bengkati Suarsana, I Made Agus Surat, Megawati R. surisna, surisna Syafika Alaydrus Tamunu, Jeine Sweet Natasya Tarusu, Fikhta Agnesya Tiara Anastasya Tien Wahyu Handayani Tien Wahyu Handayani Tien Wahyu Handayani Tien Wahyu Handayani Tien Wahyu Handayani Tien Wahyu Handayani Tien, Tien Wahyu Handayani Tonapa Rammang, Seblin Towulu, Dessy Gracelia Tuldjanah, Muthmainah Utami Islamiati Utami, Indah Kurnia Valen Ruterlin Veronika Veronika Viani Anggi Vidya Christin Wahyu Handayani, Tien Wahyu Nur Afandi WAYAN WAYAN Wayan Wirawan Wirawan W Wirawan, Resky Chandra Yana Novika R Yanti Palinggi, Ida Yanuarty, Rezky Yasinta Rakanita Yuli Fitriana Yuliet Yuliet Yulistien Yusuf Yunlis Silintowe Kenta Yusriadi Yusriadi Yustin Yustin Zulfaidah