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Uji Ekstrak Alkohol Daun Mengkudu (Morinda Citrifolia L) terhadap Konsentrasi Kreatinin dan Ureum Tikus Putih Jantan (Rattus Norvegicus) Nefropati Diabetes Tandi, Joni; R, Yana Novika; Lakiu, Debora Rislianti; Magfira; Dewi, Niluh Puspita
Jurnal Jamu Indonesia Vol. 8 No. 1 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i1.241

Abstract

Diabetic nephropathy is one of the microvascular complications of diabetes mellitus (DM). One of the clinical signs of diabetic nephropathy is an increase in urea and creatinine levels. The use of medicinal plants containing antioxidants is one of the therapeutic strategies to prevent complications of DM. The noni plant (Morinda citrifolia L), especially the leaves, contains active substances that have the potential as antioxidants to be used as supportive therapy for DM. This study aims to identify the content of active substances in noni leaf extract and determine the potential of noni leaf extract to reduce creatinine, and urea levels in diabetic nephropathy rats. Noni leaf extract was obtained by maceration using 96% ethanol, and animal models of diabetic nephropathy were obtained by induction of streptozotocin 40 mg/kg BW. The test animals were divided into six groups, namely normal control, negative control, positive control, treatment 1, 2, and 3, each given extract doses of 250, 500, and 750 mg/kg BW. The results indicated the presence of flavonoids, alkaloids, steroids, saponins, and tannins in the noni leaf extract. Giving noni leaf extract can reduce, urea and creatinine levels significantly compared to negative controls. In conclusion, noni leaf extract can potentially prevent complications of diabetic nephropathy with an effective dose of 250 mg/kg BW.
Edukasi Pemanfaatan Pembuatan Kapsul Nanopartikel dari Tanaman Pinang (Areca catechu L) sebagai Upaya Terapi Adjuvan Pencegahan Schistosomiasis di Desa Alitipu Kecamatan Lore Utara, Kabupaten Poso, Sulawesi Tengah: Education on the Utilization of Making Nanoparticle Capsules from Areca Nut Plants (Areca catechu L) as an Adjuvant Therapy Effort for Schistosomiasis Prevention in Alitipu Village, North Lore District, Poso Regency, Central Sulawesi Tandi, Joni; Magfirah, Magfirah; Kareba, Lexy; Handayani, Tien Wahyuni
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 3 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i3.8420

Abstract

Alitupu Village, located in Napu Valley, North Lore District, Poso Regency, Central Sulawesi Province has the highest prevalence rate of schistosomiasis in humans, which is around 3.36%. Praziquantel as an antischistosomiasis causes side effects such as increased SGPT and SGOT, which is an obstacle in eradicating schistosomiasis, therefore developing natural resources with plants that have been proven to have properties as anthelmintic drugs, namely areca nut in the form of nanoparticles to maximize the biological availability of drugs given orally. This community service activity was carried out for 2 days in community groups and Gapoktan farmer groups with a socialization method by providing a pre-test, posters followed by a discussion session, questions and answers, and post-test and practice of making areca nut nanoparticles and making toilets followed by measuring satisfaction with community service by giving questionnaires to a total of 50 respondents. The results of the activity, the community was very enthusiastic and could increase their knowledge through the post-test. From the pretest calculation results of 24.4 and the posttest results obtained 86.8 people know how to prevent schistosomiasis. The results of the community satisfaction assessment showed that in general partners were satisfied with the activity by 92%. It can be concluded that this PKM activity is very beneficial for the people of Alitipu Village.
POTENTIAL OF ROSELLA FLOWER EXTRACT KEFIR (HIBISCUS SABDARIFFA L.) IN REDUCING PANCREATIC β- CELL DAMAGE IN DIABETES MELLITUS MODEL RATS (RATTUS NORVEGICUS) Anggi, Viani; Tandi, Joni; D, Rahmawati; M, Yulan; L, Wahyudin H
Jurnal Farmasi Sains dan Praktis Vol 11 No 2 (May-August 2025)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v11i2.8197

Abstract

Kefir is a fermented milk product that contains probiotics, while rosella flowers (Hibiscus sabdariffa L.) are known to have antioxidant and antihyperglycemic activity. The combination of the two in the form of rosella flower extract kefir is expected to have a protective effect on the pancreas in diabetes. This study aims to evaluate the effect of administering rosella flower extract kefir preparations on the histopathological appearance of the pancreas of male white rats (Rattus norvegicus) induced by streptozotocin.This experimental study used a post-test control group design using 30 male white rats divided into several groups, namely normal controls who were given Na CMC 0.5% orally for 21 days, negative controls (induced by STZ without treatment) given Na CMC 0.5% orally for 21 days, positive controls (induced by STZ) and given glibenclamide suspension 0.45 mg/kg WB orally for 21 days, and treatment groups with different doses from kefir rosella flower extract with graded doses of 250 mg/kg BW, 500 mg/kg BW and 750 mg/kg BW. After treatment, rat pancreas was collected and analyzed histopathologically. The results of histopathological analysis showed that the group of mice that received rosella flower extract kefir at a dose of 750 mg/kg BW experienced structural improvements in pancreatic β cells with a damage value of 0.4. Administration of rosella flower extract kefir at a dose of 750 mg/kg BW has the potential to provide a protective effect on the pancreas in rats induced by streptozotocin
Ekstrak Etanol Daun Kacang Panjang (Vigna unguiculata (L.) Walp): Kajian Morfometri Insula Pankreatika Model Tikus Diabetes: Ethanol Extract of Some Cowpea (Vigna unguiculata (L.) Walp) Leaves: Morphometry Study of Pancreatic Islet of Diabetic Rat Model Solehah, Puput Octaviani; Tarusu, Fikhta Agnesya; Tandi, Joni; Dewi, Niluh Puspita; Ratih Lintin, Gabriella Bamba; Fitriana, Yuli; Pakaya, David
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 5 No. 2 (2019): (October 2019)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.118 KB) | DOI: 10.22487/j24428744.2019.v5.i2.13934

Abstract

Diabetes is characterized by an increasing of blood levels and progressive changes in the structure of pancreatic islet of pancreas. Natural ingredients that contain antioxidants such as some cowpea leaves can be an alternative treatment for diabetes. This study aims to examine the effect of ethanol extract of some cowepea leaves (Vigna unguiculata (L.) Walp) on morphometry (perimeter and diameter) of pancreatic islet of diabetic rat models. The type of research is quasi-experimental with posttest group design. Samples were 30 male wistar rats, age 8 weeks, BW 250-300 grams, that induced by single dose of STZ 40 mg/kg BW. Rats were divided into 5 groups. Group A: normal control, Group B: negative control, Group C: positive control, Group D: DM rats with 100mg/Kg BW extract therapy, Group E: DM rats with 200mg/Kg BW extract therapy, Group F: DM rats with extract therapy 300mg/Kg BW. Blood sugar levels were measured on days 7, 14 and 21. Pancreatic tissue was prepared with 5μm thickness and stained with Hematoxylin Eosin. The sample was observed under 400x magnification microscope. The observation uses Image J software to measure the perimeter and diameter of the pancreatic insula. Data were analyzed by non-parametric Kruskal Wallis and post hoct Mann Whitney statistical tests. Blood glucose level was obtained on 21st day with the highest mean in group B (246.8±156.3) and lowest in group A (67.2 ±7.85), perimeter of pancreatic islet was significantly different (p=0.026), the diameter is significantly different (p =0.046). Ethanol extract of some cowpea (Vigna unguiculata (L.) Walp) leaves with dose of 300mg/kgBB helps to restore the blood and morphometry of pancreatic islet model of diabetic rats.
Potensi Rumput Laut (Eucheuma cottonii J.Agardh) Terhadap Nefropati Diabetik Tikus Putih Jantan (Rattus norvegicus): (Potency of Seaweed (Eucheuma cottonii J.Agardh) Against Nephropathy Diabetic Male White Rats (Rattus norvegicus)) Tandi, Joni; Dewi, Niluh Puspita; Wirawan, Resky Chandra; Surat, Megawati R.
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 6 No. 2 (2020): (October 2020)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24428744.2020.v6.i2.15046

Abstract

ABSTRACT This study aims to determine the effect of a multilevel dose of ethanol extract of seaweed (Eucheuma cottonii J. Agardh) on the decreasing blood glucose levels and kidney tissue regeneration of hypercholesterolemia-diabetic male white rats. 24 rats were divided into 6 groups, namely groups 1-3 (normal control, negative control, and positive control/Glibenclamide, respectively) and groups 3-6 (seaweed extract doses of 100, 200, and 400 mg/kg BW per oral, respectively). Rats were induced by high fat diet and Streptozotocin. Histopathological picture of the kidney was observed by HE staining using 400 magnifications. Data scoring of the level of kidney damage were analyzed by One Way ANOVA at a 95% confidence level and a Least Significant Difference (LSD) test was conducted. Seaweed ethanol extract at a dose of 100 mg/kg BW was effective in reducing blood glucose levels with an average value of 122.25 mg/dL and effective in regenerating the kidney tissue of male white rats with an average value of damage of 1.9. Therefore, seaweed extract has potency to be developed and further investigated as an antidiabetic agent.
The Effect of Ethanol Extract of Guava (Psidium guajava L) Leaf on Hypercholesterolemia-Diabetic White Rats Male Induced by High Fat Feed and Streptozotocin Tandi, Joni; Diana Pratiwi, Franciska; surisna, surisna; Puspita Dewi, Niluh; Magfirah, Magfirah
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 7 No. 2 (2021): (October 2021)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24428744.2021.v7.i2.15533

Abstract

This study aims to determine the effect of guava (Psidiium guajava L) leaf extract on hypercholesterolemia-diabetic white rats male and the effective dose for reducing cholesterol and blood glucose levels. This study is a laboratory experimental study using 30 rats divided into 6 test animals. the treatment group, each group consisted of 5 rats. Group I was normal control, group II negative control was given Na-CMC suspension, group III as positive control was given simvastatin group IV dose 150 mg / kg BW, group V dose 250 mg / kg BW and group VI dose 350 mg / kg BW. The results showed that the ethanol extract of guava leaves influenced reducing blood glucose levels of male white rats with an effective dose of 250mg / kg BW with an average reduction of 119 mg/dL and effective reducing the cholesterol levels at a dose of 150 mg /kg BW with an average reduction of 28,33 mg/dL.
Total Metabolit Sekunder dan Uji Aktivitas Antioksidan Daun Juwet (Syzygium cumini L.) dengan Spektrofotometer Uv-Vis: Total Secondary Metabolites and Test of Antioxidant Activity of Juwet Leaves (Syzygium cumini L.) with Uv-Vis Spectrophotometer Tien, Tien Wahyu Handayani; Rasmiyanti, Ni Kadek Evy; Tandi, Joni; Magfirah
KOVALEN: Jurnal Riset Kimia Vol. 9 No. 3 (2023): December Edition
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2023.v9.i3.16672

Abstract

The aim of this research is to determine the total amount of secondary metabolites (alkaloids, flavonoids, saponins and tannins) contained in the ethanol juice of juwet leaves (Syzygium cumini L.) using the UV-Vis spectrophotometry method and to determine the antioxidant activity of the ethanol juice of juwet leaves based on IC50 value. Determination of total levels of secondary metabolites using UV-Vis spectrophotometry and antioxidant testing using the DPPH method. The results of qualitative analysis of the ethanol juice of juwet leaves contained secondary metabolite compounds, namely flavonoids, saponins, tannins and alkaloids. The average value obtained for the level of total secondary metabolites for flavonoid compounds was 0.417% w/w., saponins 0.766 w/w., tannins 0.282 w/w and alkaloids 0.384% w/w. The results of testing the antioxidant activity of juwet leaf ethanol extract and quercetin as a comparison had antioxidant activity, the average IC50 results were 14.289 ± 0.255 ppm and 102.45 ppm, therefore they were classified as moderate antioxidants.
Memberdayakan Masyarakat untuk Memerangi Hipertensi Melalui Program Edukasi Tanaman Herbal Joni Tandi; Tiara Anastasya; Lina Hardianti Rukmana
CARADDE: Jurnal Pengabdian Kepada Masyarakat Vol. 8 No. 1 (2025): Agustus
Publisher : Ilin Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31960/caradde.v8i1.2931

Abstract

Hipertensi merupakan masalah kesehatan yang signifikan di Sulawesi Tengah, dengan prevalensi mencapai 34,11% pada orang dewasa. Masyarakat RT09 Kelurahan Lambara menghadapi kendala dalam penanganan hipertensi, termasuk rendahnya pemahaman tentang penyakit dan pemanfaatan tanaman obat. Program pengabdian masyarakat ini bertujuan meningkatkan pemahaman warga tentang pemanfaatan tanaman obat untuk mengatasi hipertensi. Metode yang digunakan adalah participatory learning melalui ceramah interaktif dan diskusi dua arah, melibatkan 20 warga sebagai peserta. Evaluasi dilakukan menggunakan pre-test dan post-test untuk mengukur peningkatan pengetahuan. Hasil menunjukkan peningkatan signifikan dalam pemahaman peserta, dimana rata-rata pengetahuan meningkat dari 15% menjadi 96,67%. Secara spesifik, pemahaman tentang hipertensi meningkat dari 25% menjadi 100%, pengetahuan tentang tanaman obat dari 10% menjadi 95%, dan pemahaman cara kerja tanaman obat dari 10% menjadi 95%. Dapat disimpulkan bahwa program edukasi ini berhasil meningkatkan pengetahuan masyarakat tentang pemanfaatan tanaman obat untuk mengatasi hipertensi, yang dibuktikan dengan peningkatan signifikan pada semua aspek yang dievaluasi.
Efek Ekstrak Etanol Daun Pepaya Terhadap Histopatologi Ginjal Tikus Putih Yang Diinduksi Streptozotocin Yanuarty, Rezky; Tamunu, Jeine Sweet Natasya; Tandi, Joni; Dewi, Niluh Puspita
Jurnal Mandala Pharmacon Indonesia Vol. 10 No. 1 (2024): Jurnal Mandala Pharmacon Indonesia Special Issue for 18th Mulawarman Pharmaceu
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v10i1.469

Abstract

Ginjal merupakan organ yang mempunyai kemampuan menyaring darah dengan membuang limbah alami seperti urea. Limbah alami yang diperluas bisa menyebabkan kerusakan ginjal. Penelitian ini bertujuan untuk menentukan kandungan metabolits pilihan alkaloids, flavonoids, saponins, dan tanins dalam konsentrat etanol daun pepaya, dan untuk menentukan dampak dan porsi konsentrat yang layak pada melawan sel silinder ginjal pada tikus yang digerakkan oleh streptozotocin. Hewan pengerat diisolasi ke dalam 6 kelompok yang terdiri dari control tipikal, control negatif, control positif, perlakuan lepas tandan dengan dosis 100, 200, 300mg/kg BB. Penelitian selama 28 hari. Studi ini mencatat perkembangan yang terjadi pada sel tubullus ginjal tikus putih jantan yang dipicu oleh streptozotocin sebagai tingkat kerusakans dengan nilai yangs ditentukan sebagai persentase (%) pada setiap bagian tubullus yang rusak atau rusak. Hasil penelitian menunjukkans bahwa daun pepaya secara terpisah mengandung metabolits opsional alkaloids, flavonoids, saponins dan tanins. Pemberian konsentrat etanol daun pepaya dalam porsi 300mg/kg BB secara preventif bisa mempengaruhi sel silinder ginjals pada tikus yang diinduksis STZ, dengan nilais bahaya tipikal 0,4 dari batas 4.
Uji Aktivitas Antioksidan Formula Minyak Herbal Dengan Metode 1,1-Diphenyl-2-picrylhidrazil (DPPH) Wulandari, Ayu; Nurhikmah, Nurhikmah; Tandi, Joni
Jurnal Mandala Pharmacon Indonesia Vol. 10 No. 1 (2024): Jurnal Mandala Pharmacon Indonesia Special Issue for 18th Mulawarman Pharmaceu
Publisher : Program Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpi.v10i1.470

Abstract

Penelitian ini bertujuan untuk mengetahui stabilitas fisik serta aktivitas antioksidan formula minyak herbal pelita mas dengan menggunakan Metode 1,1-diphenyl-2- picrylidrazil (DPPH). Penelitian ini merupakan penelitian eksperiment laboratorium dengan rancangan untuk mengetahui nilai aktivitas antioksidan dengan menggunakan metode 1.1-Diphenyl-2-picrylhidrazil (DPPH) dan mengetahui stabilitas dari formula minyak herbal pelita mas. Analisis data menggunakan One Way Anova pada program SPSS. Hasil penelitian menunjukkan bahwa stabilitas formula minyak herbal seperti organoleptik, viskositas, kejernihan dan pH memenuhi syarat mutu sediaan minyak. Hasil pengujian aktivitas antioksidan formula minyak dan kuarsetin diperoleh nilai IC50 sebesar 101,328 ppm dan 4,623 ppm dimana formula minyak herbal pelita mas tergolongan antioksidan sedang dan kuarsetin tergolong antioksidan kuat. Hasil uji analisis statistik yaitu uji normalitas dan uji homogenitas menyatakan bahwa data terdistribusi normal dan homogen. Hasil uji One Way Anova diperoleh nilai sig. 0.000 (<0,05) hal ini menunjukkan perbedaan yang signifikan antara formula minyak herbal dengan kuarsetin.
Co-Authors Afriani S Afrizal Agustinus Widodo Ahmad, Mirnawati Alaydrus, Syafika Alaydus, Syafika Andini, Tuti Allawiah Anggi, Viani Anita Purwantari Aril, Moh Arynesta, Teddy Asrifa Asrifa Astria, Nani Astuti, Dewi Qurrotul Ayu Martina Ayu Wulandari AYU WULANDARI Azwaajum Mutahharah Basilingan, Aprince Bella Melinda Bengkati, Stely Gracia Cinda Pradita Sudar Claresta, Jong A. Darmayanti David Pakaya David Pakaya Debora Rislianti Lakiu Dewi Astuti Dewi, Alfia Dian S. Waruwu Diana Pratiwi, Franciska Dipayana, I Made Erdy Prasetyo Fahri, Mohammad Fahruddin Fahruddin Faisal Rahmat Nugraha Fajar Alan Fatma, Nurul Feiverin Tibe Gabriella Bamba Ratih Lintin Gintu, Tri Juniver Gusti Ayu Gusti Ayu, Gusti Handayani KR Handayani, Tien W. Handayani, Tien Wahyu Handayani, Tien Wahyuni Harliwong, Dhitania Ivana Hastuti L Ida Yanti Palinggi Insani, Nabila Nurul Intan, Putu Irwan Irwan Islamiati, Utami Jong A. Claresta Kiki Rizki Handayani Kurnia Gatot Novrianto L, Wahyudin H Lakiu, Debora Rislianti Lexy Kareba Lidongi, Putri Marcelita Lina Hardianti Rukmana M, Yulan Magfira Magfirah Magfirah, Magfirah Magfiroh Magfiroh Maria Kanan Mariyani Mariyani, Mariyani Marsella, Mimi Marzela Dewi Meike Rachmawati Merue, Novalia Moh Rizky Moh. Roem Muhammad Agus Muljanto Muthi’ah H Z Muthmainah Tuldjanah Muthmainnah, Sririzqi Na'i, Alpina Nadila Nadila Nadila, Nadila Nadira Nani Astria Ni Made Irma Mariani Ni Putu Setiawati Ni Wayan Ika Purwasih Niluh Puspita Dewi Niluh Puspita Dewi Niluh Puspita Dewi Niluh Puspita Dewi, Niluh Puspita Niswatulfahriyati Niswatulfahriyati Nur Azizah Nur Ikhwan Nurhikmah Nurhikmah Nurmadinah Nurmadinah Nurul Ulzmi Ovan Nugroho Pegia Parobe, Alda Iglesias Pasang, Sisilia Baan Polakitan, Grace Lovita Polibaon, Tresya J. Puspita Dewi, Niluh Putri, Debby Aulia R, Yana Novika Rahmawati D Rahmawati Salsa Dinurrosifa Rajadenda, Afwa Al Rasmiyanti, Ni Kadek Evy Recky Patala Rezky Yanuarty Riani, Ni P. I. Riani, Ni Putu Intan Rio Mariani Risna Yanti Rizaldy Lalu Ronaldy Nobertson Rusdi, Grasela Ruterlin, Valen Safitri, Nabila Umi Salsabila, Unik Hanifah Sasdila, Sasdila Seblin Tonapa Rammang Sene, Ikhsan Hi. Amir Solehah, Puput Octaviani Sri Mulyani Sri Mulyani Sri Mulyani Sri Mulyani Sabang Suarsana, I Made Agus Sujati, Ni Wayan Surat, Megawati R. surisna, surisna Syafika Alaydrus Tampang, Rivani Tamunu, Jeine Sweet Natasya Tandy, Putri Adam Tarusu, Fikhta Agnesya Tiara Anastasya Ticoh, Febriyani Tien Wahyu Handayani Tien Wahyu Handayani Tien Wahyu Handayani Tien Wahyu Handayani Tien Wahyu Handayani Tien Wahyu Handayani Tien, Tien Wahyu Handayani Toding, Ficanata Adhiguna Tonapa Rammang, Seblin Towulu, Dessy Gracelia Tuldjanah, Muthmainah Uigianto, Imelda Utami, Indah Kurnia Valen Ruterlin Veronika Veronika Viani Anggi Vidya Christin Vivi Gretia Triposa Wahyu Handayani, Tien Wahyu Nur Afandi WAYAN WAYAN Wirawan W Wirawan, Resky Chandra wirawan, Wayan Yana Novika R Yanti Palinggi, Ida Yanuarty, Rezky Yasinta Rakanita Yuli Fitriana Yuliet Yuliet Yulistien Yusuf Yunlis Silintowe Kenta Yusriadi Yusriadi Yustin Yustin Zulfaidah