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Uji Efek Ekstrak Etanol Daun Sukun (Artocarpus altilis (Parkinson Ex F.A.Zorn) Terhadap Penurunan Kadar Glukosa Darah, Kolesterol Total Dan Gambaran Histopatologi Pankreas Tikus Putih Jantan (Rattus norvegicus) Hiperkolesterolemia-Diabetes Joni Tandi; Moh Rizky; Rio Mariani; Fajar Alan
Jurnal Sains dan Kesehatan Vol. 1 No. 8 (2017): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v1i8.73

Abstract

Penelitian ini bertujuan untuk mengetahui efek serta dosis efektif ekstrak daun sukun dalam menurunkan kadar glukosa darah, kolesterol total dan gambaran histopatologi pangkreas pada hewan uji tikus putih jantan model hiperkolesterolemia-diabetes yang diinduksi pakan tinggi lemak dan streptozotocin. Penelitian dilakukan menggunakan 35 ekor hewan uji yang dikelompokkan dalam 7 kelompok dengan perlakuan yaitu kelompok kontrol normal, kontrol negatif, kontrol positif metformin, kelompok positif sinvastatindan 3 kelompok uji dengan variasi dosis ekstrak masing-masing 100 mg/KgBB, 200 mg/KgBB, dan 400 mg/KgBB. Pengukuran kadar glukosa darah dan kadar olesterol dilakukan pada hari ke 0, 35, 42, dan 49 setelah diberi perlakuan. Data kadar glukosa darah dan kolesterol total yang diperoleh dianalisis menggunakan uji one way ANOVA pada taraf kepercayaan 95% dan dilanjutkan dengan uji Post Hoc LSD, dan data hasil skoring tingkat kerusakan pankreas dianalisis dengan uji Kruskal-Wallis dilanjutkan dengan uji Mann-Whitney untuk melihat perbedaan antar perlakuan. Hasil penelitian menunjukkan bahwa terdapat senyawa metabolit sekunder pada ekstrak etanol sukun, ekstrak daun sukun dengan dosis efektif 200 mg/kgBB memiliki efek terhadap kadar glukosa darah dan kadar kolesterol total, dosis 400 mg/kgBB regenerasi jaringan pankreas tikus.
Pola Pengobatan Penderita Schistosomiasis (Penyakit Demam Keong) Di Desa Kaduwaa Kecamatan Lore Utara Kabupaten Poso Propinsi Sulawesi Tengah Joni Tandi
Jurnal Sains dan Kesehatan Vol. 1 No. 9 (2018): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v1i9.74

Abstract

Telah dilakukan penelitian Pola Pengobatan Penderita Schistosomiasis (Penyakit Demam Keong) di Desa Kaduwaa Kecamatan Lore Utara Kabupaten Poso Propinsi Sulawesi Tengah. Penelitian ini bertujuan untuk menanggulangi penyakit schistosomiasis ditinjau dari kerasionalan pengobatan untuk menanggulangi penyakit schistosomiasis dan untuk mengetahui perbedaan pola pengobatan penderita schistosomiasis di Kaduwa dan Napu keseluruhan, berdasarkan persentase penggunaan obat. Data diolah dan dideskripsikan terlebih dahulu kemudian dianalisis dengan uji chi kuadrat. Hasil pengujian menunjukkan bahwa tidak terdapat perbedaan pola pengobatan penderita schistosomiasis di Kaduwaa dan Napu keseluruhan. Petunjuk teknis Pengobatan Penderita Schistosomiasis (Penyakit demam Keong), penggunaan obat praziquantel menurut petunjuk teknis yaitu 60mg/Kg BB diminum dua kali setiap 4-6 jam sehari, dan terhadap efek samping telah digunakan paracetamol dan CTM.
Test the Effect of Miana Leaf Ethanol Extract on Ureum and Creatinine Levels in Male White Rats Joni Tandi; Yustin Yustin; Rezky Yanuarty; Tien Wahyu Handayani
Jurnal Sains dan Kesehatan Vol. 4 No. SE-1 (2022): Spesial Edition J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v4iSE-1.1686

Abstract

This study aims to determine the content of secondary metabolites in miana leaf ethanol extract, and to determine the effective dose of miana leaf ethanol extract in reducing urea and creatinine levels in male white rats. This study used 30 rats divided into 6 treatment groups, each group consisting of 5 test animals, namely normal group, negative control, positive control, dose of 150 mg/kg BW, dose 200 mg/kg BW, and dose 250 mg/kg. kg body weight. The data obtained will then be tested for normality and homogeneity to find out the data is normally distributed and homogeneous. If the data is normally distributed and homogeneous, then it is continued using One Way Anova statistical analysis at a 95% confidence level and further LSD test is carried out. If the data obtained is not normal and homogeneous, then it is analyzed using non-parametric statistics Kruskal-Wallis test and continued with Mann Whitney further test to determine the difference between all treatments. The results showed that the ethanolic extract of miana leaves contained secondary metabolites of alkaloids, flavonoids, saponins,and tannins; miana leaf ethanol extract has an effect on reducing urea and creatinine; Miana leaf ethanol extract at a dose of 250 mg/kg BW was an effective dose in reducing urea and creatinine with an average decrease of 18.8 mg/dl and 0.64 mg/dl.
Uji Ekstrak Alkohol Daun Mengkudu (Morinda Citrifolia L) terhadap Konsentrasi Kreatinin dan Ureum Tikus Putih Jantan (Rattus Norvegicus) Nefropati Diabetes Tandi, Joni; Yana Novika R; Debora Rislianti Lakiu; Magfirah; Niluh Puspita Dewi
Jurnal Jamu Indonesia Vol. 8 No. 1 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i1.241

Abstract

Diabetic nephropathy is one of the microvascular complications of diabetes mellitus (DM). One of the clinical signs of diabetic nephropathy is an increase in urea and creatinine levels. The use of medicinal plants containing antioxidants is one of the therapeutic strategies to prevent complications of DM. The noni plant (Morinda citrifolia L), especially the leaves, contains active substances that have the potential as antioxidants to be used as supportive therapy for DM. This study aims to identify the content of active substances in noni leaf extract and determine the potential of noni leaf extract to reduce creatinine, and urea levels in diabetic nephropathy rats. Noni leaf extract was obtained by maceration using 96% ethanol, and animal models of diabetic nephropathy were obtained by induction of streptozotocin 40 mg/kg BW. The test animals were divided into six groups, namely normal control, negative control, positive control, treatment 1, 2, and 3, each given extract doses of 250, 500, and 750 mg/kg BW. The results indicated the presence of flavonoids, alkaloids, steroids, saponins, and tannins in the noni leaf extract. Giving noni leaf extract can reduce, urea and creatinine levels significantly compared to negative controls. In conclusion, noni leaf extract can potentially prevent complications of diabetic nephropathy with an effective dose of 250 mg/kg BW.
POTENSI ANTIDIABETIK EKSTRAK ETANOL DAUN MATOA(Pometia pinnata) PADA TIKUS PUTIH JANTAN Tandi, Joni; Afriani S; Nadira; Handayani KR; Wirawan W; Afrizal; Tien Wahyu Handayani
Jurnal Ilmiah Manuntung Vol 8 No 1 (2022): Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v8i1.521

Abstract

Diabetes Mellitus is a metabolic disease characterized by high blood glucose levels which is commonly called hyperglycemia due to lack of insulin secretion. One of the matoa plants (Pometia pinnata) that has been used as a traditional medicine is matoa leaves which are efficacious as diabetes mellitus. The purpose of this study was to determine the secondary metabolite compounds in the ethanol extract of matoa leaves, as well as to determine the potential dose of ethanolic extract of matoa leaves which was effective in reducing blood glucose levels and on the histopathological description of the pancreas of male white rats induced by Streptozotocin. This study used a laboratory experimental method, divided into 6 treatment groups, each group consisting of 5 rats, namely normal control, negative control, positive control, treatment groups with doses of 100, 200, and 300 mg/kg body weight. Data were analyzed using One Way ANOVA statistical test at 95% confidence level followed by LSD test to see differences between treatments. The results showed that the ethanolic extract of matoa leaves contained secondary metabolites of alkaloids, flavonoids, saponins, tannins, and steroids. at a dose of 300 mg/kg BW showed effective results in reducing blood glucose levels with an average decrease of 99.6 mg/dL and the ethanol extract of matoa leaves had the potential to regenerate pancreatic cells in streptozotocin-induced male white rats of 0. 6±0.54
PENGARUH PEMBERIAN SEDIAAN NANOPARTIKEL EKSTRAK ETANOL BIJI PINANG TERHADAP KADAR SGOT DAN SGPT TIKUS TERINFEKSI Schistosoma japonicum Ovan Nugroho Pegia; David Pakaya; Joni Tandi
Medika Tadulako: Jurnal Ilmiah Kedokteran Fakultas Kedokteran Vol. 7 No. 1 (2022): Maret
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/mtj.v7i1.607

Abstract

Hati menjadi tempat deposit dan berkembangnya telur Schistosoma japonicum (S.japonicum). Infeksi tersebut dapat memicu kerusakan hati yang diidentifikasimelalui pemeriksaan serum glutamic oxaloacetic transaminase (SGOT) dan serumglutamic pyruvic transaminase (SGPT). Biji pinang secara empiris memilikikemampuan mengobati infeksi S. japonicum. Penelitian ini bertujuan untukmengetahui efek sediaan nanopartikel ekstrak etanol biji pinang terhadap kadarSGOT dan SGPT pada model tikus terinfeksi S. japonicum. Pada penelitianmenggunakan 27 ekor tikus wistar jantan berusia 8 minggu dengan berat badan250-300 gram. Tikus dibuat menjadi model terinfeksi schistosoma dengan induksiserkaria. Tikus dibagi menjadi 9 kelompok; kontrol sehat (K1), kontrol negatif(K2), kontrol positif (K3), terapi ekstrak etanol biji pinang dosis 30 mg/kgBB(K4), terapi ekstrak etanol biji pinang dosis 60 mg/kgBB (K5), terapi ekstraketanol biji pinang dosis 120 mg/kgBB (K6),terapi sediaan nanopartikel ekstrak bijipinang dosis 30 mg/kgBB (K7), terapi sediaan nanopartikel ekstrak biji pinangdosis 60 mg/kgBB (K8), terapi sediaan nanopartikel ekstrak biji pinang dosis 120mg/kgBB (K9). Uji statistik menggunakan Saphiro wilk. Hasil penelitiandidapatkan sediaan nanopartiel ekstrak etanol biji pinang dosis 30, 60 dan 120mg/kgBB mampu memperbaiki rerata kadar SGPT dan SGOT pada tikus terinfeksiS. japonicum setelah 3 hari terapi.
Uji Efektivitas Antihiperglikemia Daun Kelor (Moringa Oleifera Lam.) dan Gambaran Histopatologi Pankreas Tikus Putih Jantan (Rattus Norvegicus) yang Diinduksi Streptozotocin Tandi, Joni; Yanti Palinggi, Ida; Tonapa Rammang, Seblin; Wahyu Handayani, Tien
Jurnal Jamu Indonesia Vol. 4 No. 2 (2019): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v4i2.131

Abstract

Penelitian ini bertujuan untuk menguji efek penurunan kadar glukosa darah dan gambaran histopatologi pankreas dari daun kelor pada tikus putih jantan, serta senyawa metabolit sekunder pada ekstrak fraksi n-heksan, fraksi etil asetat dan fraksi etanol-air. Pengujian menggunakan 35 ekor tikus dibagi menjadi tujuh kelompok dengan perlakuan yaitu kelompok kontrol normal, kontrol negatif, kontrol positif metformin, kontrol positif simvastatin, dan kelompok uji (fraksi n-heksan, fraksi etil asetat dan fraksi etanol air) dengan dosis 300 mg/kg BB. Pengukuran kadar glukosa darah dilakukan pada hari ke-0, 35, 42 dan 49 setelah diberi perlakuan. Hasil penelitian menunjukkan bahwa terdapat senyawa metabolit sekunder fraksi etil asetat dan etanol air yaitu alkaloid, flavonoid, saponin, tannin dan steroid. Fraksi n-heksan hanya terdapat saponin. Fraksi etanol air ekstrak daun kelor pada dosis 300 mg/kg BB yang memiliki efek penurunan kadar glukosa darah sebesar 114,6 (mg/dL) dan nilai kerusakan pancreas rata-rata 2. Lebih baik dibandingkan dengan fraksi n-heksan dan etil asetat.
Uji Ekstrak Alkohol Daun Mengkudu (Morinda Citrifolia L) terhadap Konsentrasi Kreatinin dan Ureum Tikus Putih Jantan (Rattus Norvegicus) Nefropati Diabetes Tandi, Joni; R, Yana Novika; Lakiu, Debora Rislianti; Magfira; Dewi, Niluh Puspita
Jurnal Jamu Indonesia Vol. 8 No. 1 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i1.241

Abstract

Diabetic nephropathy is one of the microvascular complications of diabetes mellitus (DM). One of the clinical signs of diabetic nephropathy is an increase in urea and creatinine levels. The use of medicinal plants containing antioxidants is one of the therapeutic strategies to prevent complications of DM. The noni plant (Morinda citrifolia L), especially the leaves, contains active substances that have the potential as antioxidants to be used as supportive therapy for DM. This study aims to identify the content of active substances in noni leaf extract and determine the potential of noni leaf extract to reduce creatinine, and urea levels in diabetic nephropathy rats. Noni leaf extract was obtained by maceration using 96% ethanol, and animal models of diabetic nephropathy were obtained by induction of streptozotocin 40 mg/kg BW. The test animals were divided into six groups, namely normal control, negative control, positive control, treatment 1, 2, and 3, each given extract doses of 250, 500, and 750 mg/kg BW. The results indicated the presence of flavonoids, alkaloids, steroids, saponins, and tannins in the noni leaf extract. Giving noni leaf extract can reduce, urea and creatinine levels significantly compared to negative controls. In conclusion, noni leaf extract can potentially prevent complications of diabetic nephropathy with an effective dose of 250 mg/kg BW.
Edukasi Pemanfaatan Pembuatan Kapsul Nanopartikel dari Tanaman Pinang (Areca catechu L) sebagai Upaya Terapi Adjuvan Pencegahan Schistosomiasis di Desa Alitipu Kecamatan Lore Utara, Kabupaten Poso, Sulawesi Tengah: Education on the Utilization of Making Nanoparticle Capsules from Areca Nut Plants (Areca catechu L) as an Adjuvant Therapy Effort for Schistosomiasis Prevention in Alitipu Village, North Lore District, Poso Regency, Central Sulawesi Tandi, Joni; Magfirah, Magfirah; Kareba, Lexy; Handayani, Tien Wahyuni
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 3 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i3.8420

Abstract

Alitupu Village, located in Napu Valley, North Lore District, Poso Regency, Central Sulawesi Province has the highest prevalence rate of schistosomiasis in humans, which is around 3.36%. Praziquantel as an antischistosomiasis causes side effects such as increased SGPT and SGOT, which is an obstacle in eradicating schistosomiasis, therefore developing natural resources with plants that have been proven to have properties as anthelmintic drugs, namely areca nut in the form of nanoparticles to maximize the biological availability of drugs given orally. This community service activity was carried out for 2 days in community groups and Gapoktan farmer groups with a socialization method by providing a pre-test, posters followed by a discussion session, questions and answers, and post-test and practice of making areca nut nanoparticles and making toilets followed by measuring satisfaction with community service by giving questionnaires to a total of 50 respondents. The results of the activity, the community was very enthusiastic and could increase their knowledge through the post-test. From the pretest calculation results of 24.4 and the posttest results obtained 86.8 people know how to prevent schistosomiasis. The results of the community satisfaction assessment showed that in general partners were satisfied with the activity by 92%. It can be concluded that this PKM activity is very beneficial for the people of Alitipu Village.
POTENTIAL OF ROSELLA FLOWER EXTRACT KEFIR (HIBISCUS SABDARIFFA L.) IN REDUCING PANCREATIC β- CELL DAMAGE IN DIABETES MELLITUS MODEL RATS (RATTUS NORVEGICUS) Anggi, Viani; Tandi, Joni; D, Rahmawati; M, Yulan; L, Wahyudin H
Jurnal Farmasi Sains dan Praktis Vol 11 No 2 (May-August 2025)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v11i2.8197

Abstract

Kefir is a fermented milk product that contains probiotics, while rosella flowers (Hibiscus sabdariffa L.) are known to have antioxidant and antihyperglycemic activity. The combination of the two in the form of rosella flower extract kefir is expected to have a protective effect on the pancreas in diabetes. This study aims to evaluate the effect of administering rosella flower extract kefir preparations on the histopathological appearance of the pancreas of male white rats (Rattus norvegicus) induced by streptozotocin.This experimental study used a post-test control group design using 30 male white rats divided into several groups, namely normal controls who were given Na CMC 0.5% orally for 21 days, negative controls (induced by STZ without treatment) given Na CMC 0.5% orally for 21 days, positive controls (induced by STZ) and given glibenclamide suspension 0.45 mg/kg WB orally for 21 days, and treatment groups with different doses from kefir rosella flower extract with graded doses of 250 mg/kg BW, 500 mg/kg BW and 750 mg/kg BW. After treatment, rat pancreas was collected and analyzed histopathologically. The results of histopathological analysis showed that the group of mice that received rosella flower extract kefir at a dose of 750 mg/kg BW experienced structural improvements in pancreatic β cells with a damage value of 0.4. Administration of rosella flower extract kefir at a dose of 750 mg/kg BW has the potential to provide a protective effect on the pancreas in rats induced by streptozotocin
Co-Authors Afriani S Afrizal Agustinus Widodo Alaydrus, Syafika Alfia Dewi Anita Purwantari Asrifa Asrifa Astuti, Dewi Qurrotul Ayu Martina Ayu Wulandari AYU WULANDARI Ayu Wulandari Azwaajum Mutahharah Basilingan, Aprince Bella Melinda Cinda Pradita Sudar Claresta, Jong A. Darmayanti David Pakaya David Pakaya Debora Rislianti Lakiu Dewi Astuti Dhitania Ivana Harliwong Dian S. Waruwu Diana Pratiwi, Franciska Erdy Prasetyo Fahruddin Fahruddin Faisal Rahmat Nugraha Fajar Alan Feiverin Tibe Ficanata Adhiguna Toding Gabriella Bamba Ratih Lintin Gusti Ayu Gusti Ayu, Gusti Handayani KR Handayani, Tien Wahyu Handayani, Tien Wahyuni Hastuti L I Made Dipayana Ida Yanti Palinggi Irwan Irwan Islamiati, Utami Jong A. Claresta Kiki Rizki Handayani Kurnia Gatot Novrianto L, Wahyudin H Lakiu, Debora Rislianti Lexy Kareba Lidongi, Putri Marcelita Lina Hardianti Rukmana M, Yulan Magfira Magfira Magfirah Magfirah, Magfirah Magfiroh Magfiroh Maria Kanan Mariyani Mariyani, Mariyani Marsella, Mimi Marzela Dewi Meike Rachmawati Moh Rizky Moh. Roem Muhammad Agus Muljanto Muthi’ah H Z Na'i, Alpina Nadila Nadila Nadira Nani Astria Ni Made Irma Mariani Ni Putu Intan Riani Ni Putu Setiawati Ni Wayan Ika Purwasih Niluh Puspita Dewi Niluh Puspita Dewi Niluh Puspita Dewi Niluh Puspita Dewi, Niluh Puspita Niswatulfahriyati Niswatulfahriyati Novalia Merue Nur Azizah Nur Ikhwan Nurhikmah Nurhikmah Nurmadinah Nurmadinah Nurul Ulzmi Ovan Nugroho Pegia Parobe, Alda Iglesias Pasang, Sisilia Baan Puspita Dewi, Niluh Putu Intan R, Yana Novika Rahmawati D Rasmiyanti, Ni Kadek Evy Recky Patala Rezky Yanuarty Rio Mariani Risna Yanti Rivani Tampang Rizaldy Lalu Ronaldy Nobertson Ruterlin, Valen Salsabila, Unik Hanifah Sasdila, Sasdila Seblin Tonapa Rammang Solehah, Puput Octaviani Sri Mulyani Sri Mulyani Sri Mulyani Sri Mulyani Sabang Sririzqi Muthmainnah Stely Gracia Bengkati Suarsana, I Made Agus Surat, Megawati R. surisna, surisna Syafika Alaydrus Tamunu, Jeine Sweet Natasya Tandy, Putri Adam Tarusu, Fikhta Agnesya Tiara Anastasya Tien Wahyu Handayani Tien Wahyu Handayani Tien Wahyu Handayani Tien Wahyu Handayani Tien Wahyu Handayani Tien Wahyu Handayani Tien, Tien Wahyu Handayani Tonapa Rammang, Seblin Towulu, Dessy Gracelia Tuldjanah, Muthmainah Utami Islamiati Utami, Indah Kurnia Valen Ruterlin Veronika Veronika Viani Anggi Vidya Christin Wahyu Handayani, Tien Wahyu Nur Afandi WAYAN WAYAN Wayan Wirawan Wirawan W Wirawan, Resky Chandra Yana Novika R Yanti Palinggi, Ida Yanuarty, Rezky Yasinta Rakanita Yuli Fitriana Yuliet Yuliet Yulistien Yusuf Yunlis Silintowe Kenta Yusriadi Yusriadi Yustin Yustin Zulfaidah