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All Journal Jurnal Veteriner Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Biosains Pascasarjana Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Biotechnology Biosaintifika: Journal of Biology & Biology Education Jurnal Medik Veteriner Journal of Aquaculture Science Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Journal of Aquaculture and Fish Health Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Journal of Fisheries & Marine

Nunuk Dyah Retno Lastuti

Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Kampus C Unair, Jl. Mulyorejo, Kota Surabaya, Jawa Timur, Indonesia 60116

Author-ID : 676897

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Energy Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Prevalence and Severity of Scabies (Sarcoptes scabiei) on Rabbits in Kuala Lumpur City Veena Vasheene Vijaya Kumar; Mustofa Helmi Effendi; Nunuk Dyah Retno Lastuti; Nusdianto Triakoso; Maudika Pamela Fiorenza
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

This study intends to know the prevalence and crusting severity of scabies (Sarcoptes scabiei) on Rabbits in Kuala Lumpur Rabbit Farm. Sarcoptes scabiei is a mite that has a predilection in the stratum corneum of the skin and caused scabies. Clinical symptoms were characterized by alopecia and crusting in the skin. This study presents a laboratory explorative research with a cross-sectional approach. Samples used rabbits in the farm that showed the clinical symptoms of scabies and the result of scraping skin in a native examination at the laboratory. Rabbits that positive scabies then performed scoring by the severity based on the region of the body and clinical symptoms. Results showed that from 140 rabbits in the population, the positive result is 13 rabbits were infected by scabies and there were 2 grades of crusting severity of scabies consisting of mild and moderate grade with separated 2 regions of the body. The low grade showed the alopecia and crusting in the face region while the medium grade in the face and foot region. The conclusion of this study is the number of prevalence scabies is 9.29% and crusting severity that low grade is 69.32% and medium grade is 30.78%.

Anthelmintic Activity Test of Pare Leaves Extract (Momordica charantia L.) Against Worms Raillietina sp. by In Vitro Trifena Pristi Anindyta; Setiawan Koesdarto; Sri Agus Sudjarwo; Lilik Maslachah; mufasirin mufasirin; Nunuk Dyah Retno Lastuti; Lutfiah Annisa Billa
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this research is to prove that pare leaf extract (Momordica charantia L.) has anthelmintic activity against Raillietina sp. in vitro. This study consisted of four treatments and each treatment contained six replications, each of which consisted of six worms. The treatments in this research were K- with 1% CMC-Na, P1 with 2% extract concentration, P2 with 4% extract concentration, P3 with 8% extract concentration. Pare leaf extract has anthelmintic activity against Raillietina sp. in vitro with an effective dose of 8%. The higher the concentration of pare leaf extract, the higher the mortality rate of worms. The longer the immersion time, the higher the number of dead worms.

Intensitas dan Prevalensi Cacing Ektoparasit Pyragraphorus hollisae pada Ikan Bawal Bintang (Tranchinotus blochii) di Perairan Lampung, Sumatera, Indonesia Rizhar Eman Karunia Akbar; Sri Subekti; Nunuk Dyah Retno Lastuti
Journal of Aquaculture Science Vol 7 No 1 (2022): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v7i1.206

Pomfret (Tracinotus blochii) is a new commodity currently the mainstay of Indonesian marine aquaculture. Pomfret's exports totaled 2,150 thousand tons in 2018. From 2017 to 2018, this number increased by 100 percent from the export volume produced in Indonesia, which was 1,075 thousand tons. Of course, there are problems that are frequently encountered in the development of cultivation, such as the attack of ectoparasite worms. In this study, the silver pompano was found to be infested with the Pyragraphorus hollisae worm. The purpose of this study is to determine the prevalence and intensity of the ectoparasite worm Pyragraphorus hollisae in silver pompano (Trachinotus blochii) in Lampung waters, Sumatra, Indonesia. This is an experimental laboratory study, and the study design is a cross-sectional study. The purposive sampling method was used to collect silver pompano. The intensity and prevalence of the P. hollisae ectoparasite worm that infested the silver pompano (Tracinotus blochii) in Lampung waters were the main parameters observed. Physical and chemical parameters are used as supporting parameters in this study. In the light category, the P. hollisae ectoparasite worm that infested silver pompano from Lampung waters had an intensity value of 1.38 individuals/head. The prevalence of P. hollisae worms in silver pompano (Trachinotus blochii) in Lampung waters was 86.7 percent in the usual category.

Application of the Cytochrome-b gene region in Population Study of Striped Snakehead (Channa striata) by Molecular Approach Sapto Andriyono; Muhammad Chaniful Fuadi; Nunuk Dyah Retno Lastuti
Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Vol 10, No 2 (2022): Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan
Publisher : UNIVERSITAS MUHAMMADIYAH PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29406/jr.v10i2.3915

Snakehead fish with different geographical differences can have an impact on genetic diversity. DNA barcoding is a system designed to identify a species precisely and accurately by using short and standardized gene regions. This study aims to determine the diversity of snakehead fish populations in four areas in East Java. This study used the Cytochrome b (Cyt b) gene sequence in mitochondrial DNA to determine genetic variation in the wild population of Channa striata. The results of DNA barcodes with Cytochrome c oxidase subunit I (COI) showed that all samples were validly C. striata species (99.84-99.85%), while Cyt-b sequences had percentage identity with the same sequence species ranging from 99.74 to 100%. The phylogenetic tree reconstructed using MEGAX resulted in a separate clade in the sequences from East Java. This strengthens the similarity of the population of C. striata species in the Brantas watershed.

Morphometric and Meristic Analysis of Rasbora in East Java Province P Kumaladewi; Mufasirin; Nunuk Dyah Retno Lastuti; Mochammad Amin Alamsjah; Win Darmanto; Sapto Andriyono
Journal of Aquaculture and Fish Health Vol. 11 No. 3 (2022): JAFH Vol. 11 No. 3 September 2022
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v11i3.30956

Rasbora (Rasbora sp.) is one of the endemic freshwater fish species in East Java, which is currently being threatened due to environmental degradation, human activities and pollution. In East Java, it is known that there are several species of Rasbora (Rasbora sp.). Domestication is one method to preserve Rasbora in East Java, preceded by identifying potential species of Rasbora sp. from the natural catchment. This study aims to determine the diversity of Rasbora sp in East Java. Samples were taken at five different locations, specifically: Nganjuk (Brantas Watersheds), Ngawi (Bengawan Solo Watersheds, Silowo Springs Tuban, Umbulan Springs Pasuruan and the Setail Watersheds Banyuwangi. The main parameters observed were morphology and morphometry. The morphological analysis used identification guidelines, while morphometric data were obtained through 15 parameters measurement then analyzed using one-way Anova test on SPSS 25 software. Based on the morphological and morphometric analysis, it is known that the species of Rasbora in Nganjuk and Ngawi regencies are Rasbora argyrotaenia and the species of Rasbora in Tuban, Pasuruan and Banyuwangi regencies are Rasbora lateristriata. One-way Anova test showed Rasbora from the five locations were significantly different based on the result value of p<0.05.

Identification Of Gastrointestinal Protozoa Of Swine (Sus sp.) In Surabaya Slaughterhouse In Dry Season Amalia Rosydinasari Rosydinasari; Nunuk Dyah Retno Lastuti; Ira Sari Yudaniayanti; Mufasirin
Journal of Parasite Science (JoPS) Vol. 7 No. 1 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i1.44133

Surabaya slaughterhouse provides pork cuts from several swine farms. Prior to slaughter, the swine were placed in a temporary pen which allows the transmission of gastrointestinal protozoa to fellow swine and to humans at the Surabaya slaughterhouse. This research was conducted to identify gastrointestinal protozoa of swine that slaughtered in Surabaya slaughterhouse. Total of 100 fecal samples was observed using native method, sedimentation method, Fulleborne floating method and Ziehl neelsen method. Based on the results, there were 47 samples positive. The protozoa found included Balantidium sp. with 46% prevalence, Eimeria sp. / Isospora sp. with 32% prevalence, Entamoeba sp. with 24% prevalence, and Blastocystis sp.with 12% prevalence.

Correlation of Nitrite and Ammonia with Prevalence of Enterocytozoon hepatopenaei (EHP) in Shrimp (Litopenaeus vannamei) on Several Super-Intensive Ponds in East Java, Indonesia Anord Charles Nkuba; Gunanti Mahasri; Nunuk Dyah Retno Lastuti; Adamu Ayubu Mwendolwa
Jurnal Ilmiah Perikanan dan Kelautan Vol. 13 No. 1 (2021): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v13i1.24430

AbstractEnterocytozoon hepatopenaei (EHP), a microsporidia parasite known to hinder shrimp growth by infecting its hepatopancreas, is recently an emerging infection for Litopenaeus vannamei farms in Indonesia. The present study was conducted to investigate the relationship between nitrite and ammonia concentration with EHP infection in the super-intensive ponds. Shrimps and water samples were collected from six ponds in aquaculture super-intensive ponds with a stocking density of 5,000 shrimps in each pond which is located in Pasuruan, Lamongan, and Tuban Regency, Indonesia. The water sample was taken to the laboratory for the measurement of ammonia and nitrite. The samples of ammonia and nitrite were detected by spectrophotometer, and the PCR was used to detect 18S rRNA of EHP. The PCR analysis that showed two positive samples of L. vannamei, from the ponds with a high concentration of ammonia and nitrite, were infected by EHP. The statistical analysis showed a significant correlation between ammonia and nitrite with the prevalence of EHP infections, where the Pearson correlation (r) was 0.980 and 0.943, respectively. There was a high prevalence of EHP infection with the increase in nitrite concentration and ammonia in pond four and pond six. The concentration of over 1mg/l of ammonia and nitrite could influence EHP infection prevalence in the shrimp farms. Highlight ResearchAmmonia and nitrite concentration influence the prevalence of Enterocytozoon hepatopenaei infection in Super intensive ponds.The estimated prevalence of Enterocytozoon hepatopenaei was 16.7%.PCR amplification analysis was used to detect the 18S ss-rRNA of Enterocytozoon hepatopenaei and 510-bp was obtained.

The Effect of Immersion Duration of Zoothamnium penaei Crude Protein Extracts to Stimulate Immune System in Litopenaeus vannamei Against Enterocytozoon hepatopenaei (EHP) Anord Charles Nkuba; Gunanti Mahasri; Nunuk Dyah Retno Lastuti; Adamu Ayubu Mwendolwa
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i2.38349

Highlight Research The effect of immersion duration of shrimp to the immunostimulant from crude protein Zoothamnium penaei was determined. 20-minute immersion duration in crude protein from Zoothamnium penaei was found to increase the phagocytosis activities, phenoloxidase, and improve hepatopancreases of the shrimp. There is increase in phagocytosis activities, phenoloxidase and improved hepatopancreases cell within a three-week maintenance of shrimp with immersion after each seven days. There is interaction between immersion duration and maintenance time of shrimp into the crude protein from Zoothamnium penaei. Abstract Enterocytozoon hepatopenaei (EHP) is a spore-forming microsporidia intracellular parasite, which slough off the cells of the hepatopancreases of the shrimp. This study was intended to determine the effect of immersion duration and maintenance time to stimulate the immune system of shrimps by using a crude protein from Zoothamnium penaei to protect against EHP infections. The study used a completely randomized design where shrimps were divided into four groups each having five replicates. Groups P1, P2, P3 were immersed in immunostimulant for 10, 15, and 20 minutes respectively, except negative control. The phenoloxidase, phagocytosis activities, histopathology study were determined. Also, the molecular diagnosis of EHP was measured to confirm EHP infection in the shrimp samplesThe phenoloxidase activity significantly increased after immersion for 20 minutes (P3) from 0.64-, 0.72-, and 0.78-units min-1 mil -1 of protein on the first week, second week and third week, respectively. The phagocytosis activities of the sample immersed for 20 minutes were significantly increased to 68% on the second week. The sample also showed improved hepatopancreases epithelial cells with only 26% necrotic cell, less EHP spore and vacuolation. There was an interaction between immersion duration and maintenance time of the L. vannamei immersed in crude protein from Zoothamnium penaei. Twenty minutes immersion were significantly found to stimulate the immune system of the shrimp against EHP. The present work revealed that, the application of crude protein from Z. penaei is effective against EHP in shrimp culture.

Prevalence and Infestation of Ectoparasite in Dabung Ducks in Some Districts in Bangkalan Madura Prawasa, Ramadhiniyanti Putri Alif; Nunuk Dyah Retno Lastuti; Dadik Raharjo; Poedji Hastutiek; Endang Suprihati; Dian Ayu Permatasari
Journal of Parasite Science Vol. 7 No. 2 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i2.40134

Identify the type of ectoparasite prevalence and infestation pattern of ectoparasite on Dabung duck in Bangkalan Madura regency. This research conducted from February to April 2022 with sample of 55 tails and identified in parasitology laboratory of the Veterinary Parasitology Division, Faculty of Veterinary Medicine, Airlangga University, Surabaya with the permanent mounting method without coloring. This research was a descriptive research, study survey, and cross sectional study analytical. The type of ectoparasite that attacked Dabung duck in Geger subdistrict, Dabung village and Galis subdistrict, Pekaan Dajah village were Menacanthus stramineus, Menopon gallinae, Lipeurus caponis with a prevalence of 100% from a sample 55 tails of Dabung duck. The analysis results of infestation pattern of ectoparasite infestation on Dabung ducks used correspondence text on the body regions of Dabung ducks in Geger subdistrict, Dabung village showed that M. stramineus ticks on chest region, M. gallinae in head neck region and L. caponis in wing region. Burneh subdistrict, Burneh village founded M. stramineus ticks in abdomen region, M. gallinae in head-neck region, and L. caponis in wing region. Galis subdistrict, Pakaan Dajah village showed that M stramineus lice in head-neck region, L. caponis in wing region and M. gallinae in spine region.

MOLECULAR IDENTIFICATION OF SARCOPTES SCABIEI VAR. CUNICULI FROM SURABAYA AND MALANG REGIONS OF EAST JAVA Desiandura, Kurnia; Lastuti, Nunuk Dyah Retno; Suwanti, Lucia Tri; Handijatno, Didik
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 6 (2017)
Publisher : Institute of Topical Disease Universitas Airlangga

Scabies is a zoonotic skin disease caused by Sarcoptes scabiei mites. As an emerging/re-emerging parasitic disease, scabies represents a significant global threat to both human and animal health. Numerous cases of scabies in Indonesia have been reported, which support research on the prevalence of S. scabiei. However, most such studies have involved conventional morphological studies, with limited molecular diagnostic studies. The purpose of the present study was the genetic characterization of S. scabiei var. cuniculi in domestic rabbits to generate baseline genotypic data. S. scabiei var. cuniculi was isolated and identified from scabies-infected rabbits from the Surabaya and Malang regions of East Java. Molecular identification was performed using Polymerase Chain Reaction (PCR) using specific primers targeting the COX1 gene. We performed COX1 PCR using rabbit isolates of S. scabiei from Indonesia. To the best of our knowledge, no such study had been reported previously. This study was performed in the Laboratory of Veterinary Parasitology, Faculty of Veterinary Medicine and the Tropical Disease Diagnostic Center Laboratory, Universitas Airlangga. The results with agarose gel electrophoresis revealed a 289 bp PCR product amplified from the DNA of S. scabiei isolates from both Surabaya and Malang in accordance with the expected COX1 amplicon size, that indicated a single band 289 bp in length, demonstrating specific detection of S. scabiei var. cuniculi from Surabaya and Malang using COX1 primers. The results were consistent with the calculated amplicon size based on primer positions within the COX1 locus, with the forward primer spanning nucleotides 61–94, and the reverse primer spanning nucleotides 331–350 ( 350 âˆ’ 61 = 289 bp). PCR genotyping of the isolates yielded an identical nucleotide length of 289 bp. Further studies are required to sequence the amplified fragments for homology assessment.

Co-Authors Abdullah Abdullah Adamu Ayubu Mwendolwa Adamu Ayubu Mwendolwa Afiat Berbudi Agnes Theresia Soelih Estoepangestie Agustina, Firanda Ahmad Ghiffari Aldi Hamdani Amalia Rosydinasari Rosydinasari Amirotul Azhimah Amirul Muslim Amrullah Anam Al Arif Anord Charles Nkuba Bambang Sektiari Lukiswanto Chairil Anwar Chaniful Fuadi, Muhammad Dadik Rahardjo, Dadik Dadik Raharjo Damayanti, Tita Desiandura, Kurnia Dian Ayu Permatasari Didik Handijatno Diyah Ayu Candra Doohan Mahendra Dwi Ananda, Novita Dwi Handayani Dyah Ayu Kurniawati E Djoko Poetranto Ellza Agatha Damayanti Emy Koestanti Sabdoningrum Endang Suprihati Erma Safitri Fadila Zikri Amanda Fatmawati, Mira Fatmawati Fedik Abdul Rantam Fransiska Cicilia Beka Gandul Atik Yuliani Gita Dwi Prasasty Gunanti Mahasri Hana Eliyani Hartono Hartono Hidajanti, Nove Inda Astri Aryani Ira Sari Yudaniayanti Kadek Rachmawati Koesdarto Koesdarto Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lianny Nagoi Lilik Maslachah Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Luluk Tri Astuti Lutfiah Annisa Billa Marchelia Arifiandani Masdiana C Padaga Maudika Pamela Fiorenza Meles, Dewa Ketut Meyreta Doti Alcaterana Miftahurrizqiyah Miftahurrizqiyah Mirni Lamid Mochamad Lazuardi Mochammad Amin Alamsjah Moh. Ilham Rizkul Ulum Muchammad Yunus Mufasirin Muhamad Amin Muhammad Athoillah Sholahuddin Muhammad Chaniful Fuadi Muhammad Fahmi Abdillah Muhammad Rofi' Prasetya Mustofa Helmi Effendi Ni Komang Aprilina Widisuputri Nisa’ Rachmaningtyas Putri Nove Hidayati Nusdianto Triakoso P Kumaladewi Pinatih, Ayu Komang Ria Trie Dewi Poedji Hastutiek Prasetya, Muhammad Rofi' Pratiwi, Arif Prawasa, Ramadhiniyanti Putri Alif Rahmi Sugihartuti Retno Bijanti Rizhar Eman Karunia Akbar Rochmah Kurnijasanti Romy Muhammad Dary Mufa Sapto Andriyono Setiawan Koesdarto Soeharsono Soeharsono Sri Agus Sudjarwo Sri Subekti Sunarso, Agus Suzanita Utama Taufik Tri Laksono Tjuk Imam Restiadi Trifena Pristi Anindyta Veena Vasheene Vijaya Kumar Vindo Rossy Pertiwi Warsito, Sunaryo Hadi Widi Nugroho Widiyatno, Thomas Valentinus Win Darmanto Wiwik Misaco Yuniarti Wurlina, W Yeni Dhamayanti Yunus, Muchammad Yunus, Muchammad

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