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All Journal Jurnal Veteriner Jurnal Biosains Pascasarjana Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Biotechnology Biosaintifika: Journal of Biology & Biology Education Jurnal Medik Veteriner Journal of Aquaculture Science Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Journal of Aquaculture and Fish Health Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Journal of Fisheries & Marine
Nunuk Dyah Retno Lastuti
Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Kampus C Unair, Jl. Mulyorejo, Kota Surabaya, Jawa Timur, Indonesia 60116
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Energy Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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EFFECT OF DIFFERENCES BROMELAIN ENZYME CONSENTRATION ON PROTEIN HYDROLYSATE FROM WASTE OF TILAPIA VISCERA (Oreochromis sp.) ON ANTIOXIDANT ACTIVITY Muhammad Athoillah Sholahuddin; Nunuk Dyah Retno Lastuti; Muhamad Amin
Jurnal Biosains Pascasarjana Vol. 26 No. 1 (2024): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v26i1.2024.15-22

Abstract

Tilapia is a commodity that is widely consumed by the public, especially in Indonesia. Fresh tilapia offal has a protein content composition of 55.55%, so to increase its selling value it is often made into protein hydrolysate. Protein hydrolysis using enzymes is an effective way, because it can make protein hydrolysates avoid damage to certain amino acids. Proteolytic enzymes have many types and one that can be used as a hydrolyzer, in producing protein hydrolysates is bromelain enzyme. The purpose of this study was to determine the effect of proteolytic enzyme type and hydrolysis time on antioxidant activity of protein hydrolysate from Oreochromis sp. offal waste. This study used RAL 2 factors and each was repeated 3 times. The first factor was the concentration of bromelain enzyme used, namely control concentration, 1%, 2% and 3%. The second factor was the hydrolysis time, which was 4 hours, 6 hours and 8 hours. The data obtained were analyzed and continued with antioxidant activity test, hydrolysis degree test and protein content test. The best concentration of bromelain enzyme as antioxidant was 3% concentration for 6 hours hydrolysis with IC50 value of 82.53µg/mL, the best DH of 82.25%±4.03 and the highest protein content of 54.55%. Keywords: Antioxidant, bromelain enzyme, tilapia offal, protein hydrolysate
Prevalence and Infestation of Ectoparasite in Dabung Ducks in Some Districts in Bangkalan Madura Prawasa, Ramadhiniyanti Putri Alif; Nunuk Dyah Retno Lastuti; Dadik Raharjo; Poedji Hastutiek; Endang Suprihati; Dian Ayu Permatasari
Journal of Parasite Science Vol. 7 No. 2 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i2.40134

Abstract

Identify the type of ectoparasite prevalence and infestation pattern of ectoparasite on Dabung duck in Bangkalan Madura regency. This research conducted from February to April 2022 with sample of 55 tails and identified in parasitology laboratory of the Veterinary Parasitology Division, Faculty of Veterinary Medicine, Airlangga University, Surabaya with the permanent mounting method without coloring. This research was a descriptive research, study survey, and cross sectional study analytical. The type of ectoparasite that attacked Dabung duck in Geger subdistrict, Dabung village and Galis subdistrict, Pekaan Dajah village were Menacanthus stramineus, Menopon gallinae, Lipeurus caponis with a prevalence of 100% from a sample 55 tails of Dabung duck. The analysis results of infestation pattern of ectoparasite infestation on Dabung ducks used correspondence text on the body regions of Dabung ducks in Geger subdistrict, Dabung village showed that M. stramineus ticks on chest region, M. gallinae in head neck region and L. caponis in wing region. Burneh subdistrict, Burneh village founded M. stramineus ticks in abdomen region, M. gallinae in head-neck region, and L. caponis in wing region. Galis subdistrict, Pakaan Dajah village showed that M stramineus lice in head-neck region, L. caponis in wing region and M. gallinae in spine region.
MOLECULAR IDENTIFICATION OF SARCOPTES SCABIEI VAR. CUNICULI FROM SURABAYA AND MALANG REGIONS OF EAST JAVA Desiandura, Kurnia; Lastuti, Nunuk Dyah Retno; Suwanti, Lucia Tri; Handijatno, Didik
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 6 (2017)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (752.607 KB) | DOI: 10.20473/ijtid.v6i6.5436

Abstract

Scabies is a zoonotic skin disease caused by Sarcoptes scabiei mites. As an emerging/re-emerging parasitic disease, scabies represents a significant global threat to both human and animal health. Numerous cases of scabies in Indonesia have been reported, which support research on the prevalence of S. scabiei. However, most such studies have involved conventional morphological studies, with limited molecular diagnostic studies. The purpose of the present study was the genetic characterization of S. scabiei var. cuniculi in domestic rabbits to generate baseline genotypic data. S. scabiei var. cuniculi was isolated and identified from scabies-infected rabbits from the Surabaya and Malang regions of East Java. Molecular identification was performed using Polymerase Chain Reaction (PCR) using specific primers targeting the COX1 gene. We performed COX1 PCR using rabbit isolates of S. scabiei from Indonesia. To the best of our knowledge, no such study had been reported previously. This study was performed in the Laboratory of Veterinary Parasitology, Faculty of Veterinary Medicine and the Tropical Disease Diagnostic Center Laboratory, Universitas Airlangga. The results with agarose gel electrophoresis revealed a 289 bp PCR product amplified from the DNA of S. scabiei isolates from both Surabaya and Malang in accordance with the expected COX1 amplicon size, that indicated a single band 289 bp in length, demonstrating specific detection of S. scabiei var. cuniculi from Surabaya and Malang using COX1 primers. The results were consistent with the calculated amplicon size based on primer positions within the COX1 locus, with the forward primer spanning nucleotides 61–94, and the reverse primer spanning nucleotides 331–350 ( 350 − 61 = 289 bp).  PCR genotyping of the isolates yielded an identical nucleotide length of 289 bp. Further studies are required to sequence the amplified fragments for homology assessment.
Identifikasi Molekuler Blastocystis sp. pada Monyet Ekor Panjang (Macaca fascicularis) di Taman Nasional Baluran, Situbondo, Jawa Timur Kurniawati, Dyah Ayu; Suwanti, Lucia Tri; Lastuti, Nunuk Dyah Retno; Koesdarto, Setiawan; Suprihati, Endang; Mufasirin, Mufasirin; Pratiwi, Arif
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.138-144

Abstract

Interaksi yang dekat antara monyet ekor panjang dengan manusia dapat meningkatkan risiko penularan penyakit zoonosis. Blastocystis sp. adalah protozoa gastrointestinal pada manusia dan hewan yang yang bersifat zoonosis. Penelitian ini bertujuan untuk mengidentifikasi Blastocystis sp. yang menginfeksi monyet ekor panjang melalui identifikasi molekuler. Identifikasi Blastocystis sp. pada penelitian ini menggunakan metode morfologis dan molekuler. Sebanyak 90 feses individu monyet ekor panjang Taman Nasional Baluran dilakukan pemeriksaan secara mikroskopis setelah dilakukan kultur pada Jones Medium. 28 dari sampel yang positif secara mikroskopis dilanjutkan dengan uji PCR dengan target primer barcode region yang mempunyai visualisasi 600bp. Tiga sampel dengan band positif 600bp dilanjutkan dengan squencing. Hasil sekuens diproses dalam BLAST dan MLST. Satu sampel yang terkonfirmasi sebagai Blastocystis sp. dengan infeksi campuran dari subtipe 1 alel 2 dan subtipe 3 alel 34. Hasil menunjukkan bahwa Blastocystis sp. terdapat pada monyet ekor panjang di Taman Nasional Baluran dengan prevalensi rendah.
Prevalence of Gastrointestinal Parasites in Pigs in Bali Pinatih, Ayu Komang Ria Trie Dewi; Lastuti, Nunuk Dyah Retno; Kusnoto, Kusnoto; Mufasirin, Mufasirin; Yunus, Muchammad; Rahardjo, Dadik
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.349-354

Abstract

This study aimed to identify gastrointestinal parasites in pigs in Bali. A total of 117 pig feces samples were collected in Buleleng Regency (n = 67) and Jembrana (n = 50). Samples were examined microscopically using native, sedimentation, and floating methods. The results reported the prevalence of gastrointestinal parasites infecting pigs in Bali was 94.8% (111/117) infected with protozoa, namely Eimeria sp. (90.5%), Entamoeba sp. (26.4%), Isospora suis (6.8%), and Balantidium sp. (5.1%), while 99.1% (116/117) were infected with helminths, namely Trichuris suis (71.7%), Strongyloides sp. (64.9%), Ascaris suum (49.5%), Oesophagostomum sp. (6.1%), Macracanthorhyncus sp. (2.5%), and Hyostrongylus sp. (0.8%). Based on the tree regression analysis reported that the rearing system was related to the degree of gastrointestinal parasite infection in pigs in Bali.
The Anthelmintic Activity Of Etanol Extract of Porang Tubers Against Fasciola gigantica In Vitro Agustina, Firanda; Kurnijasanti, Rochmah; Suwanti, Lucia Tri; Lastuti, Nunuk Dyah Retno; Rachmawati, Kadek; Maslachah, Lilik
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31235

Abstract

This study aims to determine the anthelmintic activity ethanol extract of porang tubers (A. oncophillus) against F. gigantica worms in vitro. There were five treatments and each treatment was done in five replications and used 10 F. gigantica. The treatments of this research were K- with CMC Na 1%, K+ with Albendazole 2.4 mg/ml, P1 with extract concentration 5%, P2 with extract concentration 10%, P3 with extract concentration 20%. The results showed that the extract of Porang tuber (A. oncophillus) had an anthelmintic effect against F. gigantica worms in vitro. In the extract with a concentration of 20%, there were anthelmintic properties that almost the same as Albendazole. The higher the extract concentration, the higher the anthelmintic properties. The longer the immersion time, the higher the number of dead worms. The morphological changes was evaluated by light microscopic examination and the results showed many histopathological changes on the morphology of F. gigantica. The results indicate that the possible use of the tubers as a potential anthelmintic against F. gigantica.
The Effectiveness of Ethanol Extract of Ketapang Leaves (Terminalia catappa L.) as a Larvicidal Against Aedes aegypti Mosquito Larvae Dwi Ananda, Novita; Rachmawati, Kadek; Lastuti, Nunuk Dyah Retno; Suwanti, Lucia Tri; Hidajanti, Nove; Meles, Dewa Ketut
Journal of Basic Medical Veterinary Vol. 12 No. 1 (2023): Journal of Basic Medical Veterinary, June 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i1.42248

Abstract

This study aimed to determine the effectiveness of the ethanol extract of ketapang leaves (Terminalia catappa) on mortality and histopathology of Ae. aegypti instar III as an effort to control Ae. aegypti as a disease vector without causing resistance to the larvae. The extraction process uses the maceration method. This type of research is a laboratory experiment with research subjects Ae. aegypti instar III of 700 individuals, which were divided into 7 treatment groups (1 negative control group using distilled water and 1% DMSO, 1 positive control group using 1000 ppm abate, ethanol extract of ketapang leaves with concentrations of 2000 ppm, 2500 ppm, 3500 ppm, 5000 ppm, 7000 ppm) and 4 repetitions. The results of this study indicate that the negative control (K-) mortality rate is 0% and the positive control (K+) mortality percentage is 100%. The results of the ethanol extract of ketapang leaves (Terminalia catappa) on the mortality of Ae. aegypti instar III in treatment one (P1) the percentage of mortality was 17%, treatment two (P2) the percentage of mortality was 21%, treatment three (P3) the percentage of mortality was 23%, treatment four (P4) the percentage of mortality was 27%, and treatment five (P5) the percentage of mortality is 42%. Histopathological observations on larvae that died after being exposed to ethanol extract of ketapang leaves (Terminalia catappa) for 24 hours damaged the basement membrane, peritropic membrane and epithelial cells. It can be concluded that the ethanol extract of ketapang leaves (Terminalia catappa) is effective as a larvicidal against Ae. aegypti instar III but can cause damage to the midgut and cause death of the larvae.
Co-Authors Abdullah Abdullah Adamu Ayubu Mwendolwa Adamu Ayubu Mwendolwa Afiat Berbudi Agnes Theresia Soelih Estoepangestie Agustina, Firanda Ahmad Ghiffari Aldi Hamdani Amalia Rosydinasari Rosydinasari Amirotul Azhimah Amirul Muslim Amrullah Anord Charles Nkuba Bambang Sektiari Lukiswanto Chairil Anwar Dadik Rahardjo, Dadik Dadik Raharjo Desiandura, Kurnia Dian Ayu Permatasari Didik Handijatno Diyah Ayu Candra Doohan Mahendra Dwi Ananda, Novita Dwi Handayani Dyah Ayu Kurniawati E Djoko Poetranto Ellza Agatha Damayanti Emy Koestanti Sabdoningrum Endang Suprihati Erma Safitri ERMA SAFITRI Fadila Zikri Amanda Fedik Abdul Rantam Fransiska Cicilia Beka Gandul Atik Yuliani Gita Dwi Prasasty Gunanti Mahasri Hana Eliyani Hartono Hartono Hidajanti, Nove Inda Astri Aryani Ira Sari Yudaniayanti Kadek Rachmawati Koesdarto Koesdarto Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lianny Nagoi Lilik Maslachah Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Luluk Tri Astuti Lutfiah Annisa Billa Marchelia Arifiandani Maudika Pamela Fiorenza Meles, Dewa Ketut Meyreta Doti Alcaterana Miftahurrizqiyah Miftahurrizqiyah Mochamad Lazuardi Mochammad Amin Alamsjah Moh. Ilham Rizkul Ulum Muchammad Yunus Mufasirin Muhamad Amin Muhammad Athoillah Sholahuddin Muhammad Chaniful Fuadi Muhammad Fahmi Abdillah Muhammad Rofi' Prasetya Mustofa Helmi Effendi Ni Komang Aprilina Widisuputri Nisa’ Rachmaningtyas Putri Nove Hidayati Nusdianto Triakoso P Kumaladewi Pinatih, Ayu Komang Ria Trie Dewi Poedji Hastutiek Prasetya, Muhammad Rofi' Pratiwi, Arif Prawasa, Ramadhiniyanti Putri Alif Rahmi Sugihartuti Retno Bijanti Rizhar Eman Karunia Akbar Rochmah Kurnijasanti Romy Muhammad Dary Mufa Sapto Andriyono Setiawan Koesdarto Soeharsono Soeharsono Sri Agus Sudjarwo Sri Subekti Sunarso, Agus Suzanita Utama Taufik Tri Laksono Tita Damayanti Tjuk Imam Restiadi Trifena Pristi Anindyta Veena Vasheene Vijaya Kumar Vindo Rossy Pertiwi Warsito, Sunaryo Hadi Widiyatno, Thomas Valentinus Win Darmanto Wiwik Misaco Yuniarti Wurlina, W Yeni Dhamayanti Yunus, Muchammad Yunus, Muchammad