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All Journal Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Biosains Pascasarjana Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Biotechnology Biosaintifika: Journal of Biology & Biology Education Jurnal Medik Veteriner Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Journal of Aquaculture and Fish Health Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Journal of Fisheries & Marine Journal of Aquaculture Science

Nunuk Dyah Retno Lastuti

Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Kampus C Unair, Jl. Mulyorejo, Kota Surabaya, Jawa Timur, Indonesia 60116

Author-ID : 676897

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Energy Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Detection of Goat Digestive Tract Protozoa Through Feces Examination in Kwanyar Sub-District, Bangkalan District Meyreta Doti Alcaterana; Poedji Hastutiek; Nunuk Dyah Retno Lastuti; Endang Suprihati; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 5 No. 2 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the prevalence and species of protozoa that infect theÂ digestive tract of goats in Kwanyar Sub-District, Bangkalan District. This study was conducted in June-July 2019 with 100 samples of stool examination in the laboratory of the Division of Parasitology, Faculty of Veterinary Medicine, Airlangga University. On examination found two types of protozoa, which were: Eimeria sp and Blastocystis sp. The results of this study showed the prevalence of 48% digestive tract protozoa. The results of statistical analysis using the Chi-Square test showed significant differences in age under one year and over one year in goats (p <0.05).

Prevalence of Gastrointestinal Protozoa on Bali Cattle in Lopok Sub-District, Sumbawa District Aldi Hamdani; Nunuk Dyah Retno Lastuti; Yeni Dhamayanti; Setiawan Koesdarto; Agus Sunarso; Poedji Hastutiek
Journal of Parasite Science (JoPS) Vol. 5 No. 2 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the prevalence of gastrointestinal protozoa on Bali cattle in Lopok Sub-District, Sumbawa District. The study was conducted from January to April 2021 by taking 100 samples of Bali cattle feces from 7 villages in Lopok Sub-District, Sumbawa District. Samples were examined by sedimentation and floating methods. Species identification was determined by the morphology of the protozoa. A total of 62 (62%) samples were positively infected with protozoa. There were 4 species of gastrointestinal protozoa found infecting Bali cattle, namely Eimeria sp., Blastocytis sp., Entamoeba sp., and Balantidium sp. Most of the protozoa found in this study were Eimeria sp. (54%) and Blastocytis sp. (5%). This study found 59 samples (59%) were infected by one type of protozoa, 3 samples (3%) were infected by two types of protozoa. Cattle aged 0-6 months (81%) had a higher prevalence rate and were more susceptible to protozoal infections than cows aged 7 months â€“ 2 years (70.3%) and more than 2 years (45.2%).

Identification and Prevalence of Digestive Tract Endoparasites of Goats in Ujungpangkah, Gresik District Muhammad Fahmi Abdillah; Nunuk Dyah Retno Lastuti; Suzanita Utama; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 5 No. 2 (2021): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the prevalence and species of endoparasite that infect the digestive tract of goats in Ujungpangkah Sub-District, Gresik District. This study was conducted in February-March 2021 with 100 samples of goatâ€™s stool. Sample examination was conducted in the laboratory of the Division of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga. In fecal examination found four types of endoparasites, which were: Eimeria sp. 62%, Blastocystis sp. 5%, Haemonchus sp. 4%, Strongyloides sp. 2% and mixed infection prevalence was 3%. This study showed a prevalence of 76% digestive tract endoparasite. The Chi-Square test showed significant differences (p <0.05) between groups of goats aged under one year (57%) and over one year (43%).

Identification of Ectoparasites and Gastrointestinal Parasites in Eurasian Tree Sparrow (Passer montanus) Moh. Ilham Rizkul Ulum; Mochamad Lazuardi; Nunuk Dyah Retno Lastuti; Poedji Hastutiek; Soeharsono Soeharsono; Amirul Muslim Amrullah
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the prevalence and identify ectoparasite and gastrointestinal parasites in Eurasian tree sparrow (Passer montanus) in Kediri Regency, East Java, Indonesia. The research was conducted in January 2021 with 100 samples of body swabs and feces of Eurasian tree sparrow. The examination was done in the Anugerah Satwa Veterinary Clinic in Kediri Regency using direct smear and flotation methods. The observations in this study showed that from 100 samples of body swabs, 38 samples (38%) were infested with ectoparasites which are Dermoglyphus sp. and Strelkoviacarus sp. and from 100 feces samples, 47 samples (47%) were infected with helminthic eggs with the single infections by Ascaridia sp. as much as 12%, Dispharynx sp. as much as 1% and mixed infections of Ascaridia sp. and Dispharynx sp. were 25%. Assessed by examination for the protozoa also found that Isospora sp. was present with prevalence of 100% of the 100 sparrow feces samples examined.

Prevalence and Severity of Scabies (Sarcoptes scabiei) on Rabbits in Kuala Lumpur City Veena Vasheene Vijaya Kumar; Mustofa Helmi Effendi; Nunuk Dyah Retno Lastuti; Nusdianto Triakoso; Maudika Pamela Fiorenza
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

This study intends to know the prevalence and crusting severity of scabies (Sarcoptes scabiei) on Rabbits in Kuala Lumpur Rabbit Farm. Sarcoptes scabiei is a mite that has a predilection in the stratum corneum of the skin and caused scabies. Clinical symptoms were characterized by alopecia and crusting in the skin. This study presents a laboratory explorative research with a cross-sectional approach. Samples used rabbits in the farm that showed the clinical symptoms of scabies and the result of scraping skin in a native examination at the laboratory. Rabbits that positive scabies then performed scoring by the severity based on the region of the body and clinical symptoms. Results showed that from 140 rabbits in the population, the positive result is 13 rabbits were infected by scabies and there were 2 grades of crusting severity of scabies consisting of mild and moderate grade with separated 2 regions of the body. The low grade showed the alopecia and crusting in the face region while the medium grade in the face and foot region. The conclusion of this study is the number of prevalence scabies is 9.29% and crusting severity that low grade is 69.32% and medium grade is 30.78%.

Anthelmintic Activity Test of Pare Leaves Extract (Momordica charantia L.) Against Worms Raillietina sp. by In Vitro Trifena Pristi Anindyta; Setiawan Koesdarto; Sri Agus Sudjarwo; Lilik Maslachah; mufasirin mufasirin; Nunuk Dyah Retno Lastuti; Lutfiah Annisa Billa
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this research is to prove that pare leaf extract (Momordica charantia L.) has anthelmintic activity against Raillietina sp. in vitro. This study consisted of four treatments and each treatment contained six replications, each of which consisted of six worms. The treatments in this research were K- with 1% CMC-Na, P1 with 2% extract concentration, P2 with 4% extract concentration, P3 with 8% extract concentration. Pare leaf extract has anthelmintic activity against Raillietina sp. in vitro with an effective dose of 8%. The higher the concentration of pare leaf extract, the higher the mortality rate of worms. The longer the immersion time, the higher the number of dead worms.

Application of the Cytochrome-b gene region in Population Study of Striped Snakehead (Channa striata) by Molecular Approach Sapto Andriyono; Muhammad Chaniful Fuadi; Nunuk Dyah Retno Lastuti
Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Vol 10, No 2 (2022): Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan
Publisher : UNIVERSITAS MUHAMMADIYAH PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29406/jr.v10i2.3915

Snakehead fish with different geographical differences can have an impact on genetic diversity. DNA barcoding is a system designed to identify a species precisely and accurately by using short and standardized gene regions. This study aims to determine the diversity of snakehead fish populations in four areas in East Java. This study used the Cytochrome b (Cyt b) gene sequence in mitochondrial DNA to determine genetic variation in the wild population of Channa striata. The results of DNA barcodes with Cytochrome c oxidase subunit I (COI) showed that all samples were validly C. striata species (99.84-99.85%), while Cyt-b sequences had percentage identity with the same sequence species ranging from 99.74 to 100%. The phylogenetic tree reconstructed using MEGAX resulted in a separate clade in the sequences from East Java. This strengthens the similarity of the population of C. striata species in the Brantas watershed.

Morphometric and Meristic Analysis of Rasbora in East Java Province P Kumaladewi; Mufasirin; Nunuk Dyah Retno Lastuti; Mochammad Amin Alamsjah; Win Darmanto; Sapto Andriyono
Journal of Aquaculture and Fish Health Vol. 11 No. 3 (2022): JAFH Vol. 11 No. 3 September 2022
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v11i3.30956

Rasbora (Rasbora sp.) is one of the endemic freshwater fish species in East Java, which is currently being threatened due to environmental degradation, human activities and pollution. In East Java, it is known that there are several species of Rasbora (Rasbora sp.). Domestication is one method to preserve Rasbora in East Java, preceded by identifying potential species of Rasbora sp. from the natural catchment. This study aims to determine the diversity of Rasbora sp in East Java. Samples were taken at five different locations, specifically: Nganjuk (Brantas Watersheds), Ngawi (Bengawan Solo Watersheds, Silowo Springs Tuban, Umbulan Springs Pasuruan and the Setail Watersheds Banyuwangi. The main parameters observed were morphology and morphometry. The morphological analysis used identification guidelines, while morphometric data were obtained through 15 parameters measurement then analyzed using one-way Anova test on SPSS 25 software. Based on the morphological and morphometric analysis, it is known that the species of Rasbora in Nganjuk and Ngawi regencies are Rasbora argyrotaenia and the species of Rasbora in Tuban, Pasuruan and Banyuwangi regencies are Rasbora lateristriata. One-way Anova test showed Rasbora from the five locations were significantly different based on the result value of p<0.05.

Identification Of Gastrointestinal Protozoa Of Swine (Sus sp.) In Surabaya Slaughterhouse In Dry Season Amalia Rosydinasari Rosydinasari; Nunuk Dyah Retno Lastuti; Ira Sari Yudaniayanti; Mufasirin
Journal of Parasite Science (JoPS) Vol. 7 No. 1 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i1.44133

Surabaya slaughterhouse provides pork cuts from several swine farms. Prior to slaughter, the swine were placed in a temporary pen which allows the transmission of gastrointestinal protozoa to fellow swine and to humans at the Surabaya slaughterhouse. This research was conducted to identify gastrointestinal protozoa of swine that slaughtered in Surabaya slaughterhouse. Total of 100 fecal samples was observed using native method, sedimentation method, Fulleborne floating method and Ziehl neelsen method. Based on the results, there were 47 samples positive. The protozoa found included Balantidium sp. with 46% prevalence, Eimeria sp. / Isospora sp. with 32% prevalence, Entamoeba sp. with 24% prevalence, and Blastocystis sp.with 12% prevalence.

Correlation of Nitrite and Ammonia with Prevalence of Enterocytozoon hepatopenaei (EHP) in Shrimp (Litopenaeus vannamei) on Several Super-Intensive Ponds in East Java, Indonesia Anord Charles Nkuba; Gunanti Mahasri; Nunuk Dyah Retno Lastuti; Adamu Ayubu Mwendolwa
Jurnal Ilmiah Perikanan dan Kelautan Vol. 13 No. 1 (2021): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v13i1.24430

AbstractEnterocytozoon hepatopenaei (EHP), a microsporidia parasite known to hinder shrimp growth by infecting its hepatopancreas, is recently an emerging infection for Litopenaeus vannamei farms in Indonesia. The present study was conducted to investigate the relationship between nitrite and ammonia concentration with EHP infection in the super-intensive ponds. Shrimps and water samples were collected from six ponds in aquaculture super-intensive ponds with a stocking density of 5,000 shrimps in each pond which is located in Pasuruan, Lamongan, and Tuban Regency, Indonesia. The water sample was taken to the laboratory for the measurement of ammonia and nitrite. The samples of ammonia and nitrite were detected by spectrophotometer, and the PCR was used to detect 18S rRNA of EHP. The PCR analysis that showed two positive samples of L. vannamei, from the ponds with a high concentration of ammonia and nitrite, were infected by EHP. The statistical analysis showed a significant correlation between ammonia and nitrite with the prevalence of EHP infections, where the Pearson correlation (r) was 0.980 and 0.943, respectively. There was a high prevalence of EHP infection with the increase in nitrite concentration and ammonia in pond four and pond six. The concentration of over 1mg/l of ammonia and nitrite could influence EHP infection prevalence in the shrimp farms. Highlight ResearchAmmonia and nitrite concentration influence the prevalence of Enterocytozoon hepatopenaei infection in Super intensive ponds.The estimated prevalence of Enterocytozoon hepatopenaei was 16.7%.PCR amplification analysis was used to detect the 18S ss-rRNA of Enterocytozoon hepatopenaei and 510-bp was obtained.

Co-Authors Abdullah Abdullah Adamu Ayubu Mwendolwa Adamu Ayubu Mwendolwa Afiat Berbudi Agnes Theresia Soelih Estoepangestie Agustina, Firanda Ahmad Ghiffari Aldi Hamdani Amalia Rosydinasari Rosydinasari Amirotul Azhimah Amirul Muslim Amrullah Anam Al Arif Anord Charles Nkuba Bambang Sektiari Lukiswanto Chairil Anwar Chaniful Fuadi, Muhammad Dadik Rahardjo, Dadik Dadik Raharjo Damayanti, Tita Desiandura, Kurnia Dian Ayu Permatasari Didik Handijatno Diyah Ayu Candra Doohan Mahendra Dwi Ananda, Novita Dwi Handayani Dyah Ayu Kurniawati E Djoko Poetranto Ellza Agatha Damayanti Emy Koestanti Sabdoningrum Endang Suprihati Erma Safitri Fadila Zikri Amanda Fatmawati, Mira Fatmawati Fedik Abdul Rantam Fransiska Cicilia Beka Gandul Atik Yuliani Gita Dwi Prasasty Gunanti Mahasri Hana Eliyani Hartono Hartono Hidajanti, Nove Inda Astri Aryani Ira Sari Yudaniayanti Kadek Rachmawati Koesdarto Koesdarto Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lianny Nagoi Lilik Maslachah Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Luluk Tri Astuti Lutfiah Annisa Billa Marchelia Arifiandani Masdiana C Padaga Maudika Pamela Fiorenza Meles, Dewa Ketut Meyreta Doti Alcaterana Miftahurrizqiyah Miftahurrizqiyah Mirni Lamid Mochamad Lazuardi Mochammad Amin Alamsjah Moh. Ilham Rizkul Ulum Muchammad Yunus Mufasirin Muhamad Amin Muhammad Athoillah Sholahuddin Muhammad Chaniful Fuadi Muhammad Fahmi Abdillah Muhammad Rofi' Prasetya Mustofa Helmi Effendi Ni Komang Aprilina Widisuputri Nisa’ Rachmaningtyas Putri Nove Hidayati Nusdianto Triakoso P Kumaladewi Pinatih, Ayu Komang Ria Trie Dewi Poedji Hastutiek Prasetya, Muhammad Rofi' Pratiwi, Arif Prawasa, Ramadhiniyanti Putri Alif Rahmi Sugihartuti Retno Bijanti Rizhar Eman Karunia Akbar Rochmah Kurnijasanti Romy Muhammad Dary Mufa Sapto Andriyono Setiawan Koesdarto Soeharsono Soeharsono Sri Agus Sudjarwo Sri Subekti Sunarso, Agus Suzanita Utama Taufik Tri Laksono Tjuk Imam Restiadi Trifena Pristi Anindyta Veena Vasheene Vijaya Kumar Vindo Rossy Pertiwi Warsito, Sunaryo Hadi Widi Nugroho Widiyatno, Thomas Valentinus Win Darmanto Wiwik Misaco Yuniarti Wurlina, W Yeni Dhamayanti Yunus, Muchammad Yunus, Muchammad

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