Suhartanto, Muhammad Rahmad
Department Of Agronomy And Horticulture, Faculty Of Agriculture, IPB University, Jalan Meranti Road, Bogor 16680, Indonesia

Published : 13 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 13 Documents
Search

Development of Rapid Vigor Test Using Urine Sugar Analysis Paper for Soybean (Glycine max L.) Seeds Jean D’amour Rukundo; M. Rahmad Suhartanto; Satriyas Ilyas
Journal of Tropical Crop Science Vol. 9 No. 01 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.01.22-30

Abstract

Seed vigor is an essential parameter of seed quality. It plays a critical value in the decision-making for the seeds to be used in agronomic and horticultural crops. The urinary sugar analysis paper (USAP) test determines seed vigor based on leakage concentration from the seed and corresponding color change of the USAP, which are obtained after soaking the seeds in water. This research was conducted from January 2021 to November 2021, intending to develop a rapid soybean vigor test using USAP, and it was composed of two experiments. Soybean seeds of the Biosoy1 variety were obtained from ICABIOGRAD (Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development). The seeds consisted of three different levels of seed lots (low, medium, and high viability with 35%, 64%, and 94% germination percentages, respectively). The first experiment was to determine moisture content and soaking period for the USAP seed vigor test. It was arranged in a completely randomized design with one factor as the combination of three seed lots, two levels of seed moisture content (10-12% and 13-14%), and four levels of the soaking period (0, 6, 8, and 10 hours). The second experiment was the optimization of the soaking period by seed number. It was arranged in a completely randomized design with one factor, which was combinations of three seed lots, three levels of seed numbers (50, 75, and 100 seeds), and the soaking period (four levels as 0, 6, 8, and 10 hours). The data were analyzed statistically by the Minitab package. The result showed that both combinations of 10-12% and 13-14% moisture content with the soaking period of 10 hours effectively differentiated the three seed lots into three vigor levels using USAP. Experiment 2 optimized the soaking period from 10 hours to 8 hours by using 100 seeds. The USAP color change was effectively significant to indicate seed lot vigor levels, mainly based on protein leakage concentration on USAP. The color changes from pale yellow-green for higher vigor seed lot to light greenish-blue for low vigor. The USAP was effective for the soybean vigor test for the seed lot with 10-14% moisture content by soaking 100 seeds into 50 ml of distilled water for 8 hours. The USAP is a promising rapid vigor test method but still needs to be developed further.
Ultrafine Bubbles Water priming to improve viability and vigor of bean (Phaseolus vulgaris) seeds Yuni Nurfiana; M. Rahmad Suhartanto; Endah Retno Palupi; Y. Aris Purwanto
Journal of Tropical Crop Science Vol. 10 No. 01 (2023): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.10.1.8-16

Abstract

Priming is a physiological technique of hydration of seed to improve metabolic processes before germination to accelerate germination and seedling growth under normal and stress conditions. This research is aimed to study the pretreatment using ultra-fine bubble (UFB) water to increase viability and vigor of seeds before planting (pre-planting) and during storage (pre-storage). This research was conducted at the Laboratory of Seed Quality Testing, IPB University, Indonesia. The research consisted of two experiments, i.e., UFB water priming to increase viability and vigor of bean seeds and its storability after priming. The first experiment was arranged using a completely randomized design with a combination of seed lot treatments (L1: initial germination percentage (GP) about 80%, L2: initial GP around 70%, and L3: initial GP around 60%) with priming (P0: no priming/control, P1: soaked in distilled water for 60 minutes, P2: distilled water for 120 minutes, P3: 8 ppm UFB water for 60 minutes, P4: 8 ppm UFB water for 120 minutes, P5: 20 ppm UFB water for 60 minutes, P6: UFB water 20 ppm for 120 minutes). The second experiment was arranged using a nested design with the main factor being the condition of the storage room and priming as the second factor nested in the main factor. The storage room condition factors consisted of KM: room conditions (temperature 28±5˚C, RH 73±7%) and AC: air-conditioned room (18±2 ̊C, RH 61±7%). The priming factor consisted of two levels, namely P0: without priming and P1: priming with 20 ppm UFB water soaked for 120 minutes. The results showed that priming treatment with UFB water 20 ppm for 120 minutes as a pre-planting treatment can increase the viability of bean seeds, particularly for seeds with low initial viability, and primed bean seeds with an initial viability of approximately 80% were able to retain their viability for 16 weeks of storage in an air-conditioned room.
Sintesis Nano Fertilizer Carbon Dots dari Kulit Pisang Nangka (Musa paradisiaca L.) yang Diperkaya Urea dan Pengujiannya pada Pertumbuhan Vegetatif (Awal) Tanaman Jagung Taqiyyah, Izzatut; Maddu, Akhiruddin; Suhartanto, M Rahmad
POSITRON Vol 15, No 1 (2025): Vol. 15 No. 1 Edition
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam, Univetsitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/positron.v15i1.86743

Abstract

Carbon dots (CDs) yang berasal dari karbon hijau merupakan salah satu contoh bahan organik yang dapat digunakan untuk membuat pupuk nano yang tidak beracun dan ramah lingkungan. Pada penelitian ini dilakukan proses sintesis nano fertilizer (pupuk nano) carbon dots yang diberi pengayaan nitrogen (N-CDs) dari prekursor kulit pisang nangka dan dilakukan pengujiannya sebagai pupuk nano pada pertumbuhan vegetatif jagung. Penelitian ini juga menguji karakteristik N-CDs dan efektivitasnya terhadap pertumbuhan vegetatif jagung. Metode yang digunakan adalah metode perbandingan langsung. Berdasarkan uji karakteristik CDs dan N-CDs, sejumlah gugus fungsi yang dimiliki bersama oleh CDs dan N-CDs adalah gugus hidroksil C(=O)OH dan sejumlah gugus fungsi lainnya seperti C=C-C, C-O-O, dan C-Cl terdapat dalam N-CDs tetapi tidak dalam CDs. Energi celah pita CDs kulit pisang nangka 40 mg/L urea 0% adalah 2,46 eV, dan CDs kulit pisang nangka 40 mg/L urea 10% adalah 3,99 eV. Cahaya sian dihasilkan oleh CDs dan N-CDs, namun warna sian kurang kuat pada N-CDs. Demikian pula, CDs 40 mg/L dan N-CDs 40 mg/L urea 10%, masing-masing menghasilkan panjang gelombang emisi 505 nm dan 496 nm berdasarkan uji Confocal Laser Scanning Microscope (CLSM). Secara signifikan kinerja N-CDs menunjukkan pengaruh yang lebih baik dibandingkan perlakuan CDs dan urea pada pertumbuhan vegetatif jagung.